Search

Your search keyword '"Bland, Jackson"' showing total 7 results
Start Over Author "Bland, Jackson"
7 results on '"Bland, Jackson"'

1. A genome-wide cytotoxicity screen of cluster F1 mycobacteriophage Girr reveals novel inhibitors of Mycobacterium smegmatis growth

Author

Pollenz, Richard S, primary, Barnhill, Kaylee, additional, Biggs, Abbigail, additional, Bland, Jackson, additional, Carter, Victoria, additional, Chase, Michael, additional, Clark, Hayley, additional, Coleman, Caitlyn, additional, Daffner, Marshall, additional, Deam, Caitlyn, additional, Finocchiaro, Alyssa, additional, Franco, Vanessa, additional, Fuller, Thomas, additional, Pinera, Juan Gallardo, additional, Horne, Mae, additional, Howard, Zoe, additional, Kanahan, Olivia, additional, Miklaszewski, Christopher, additional, Miller, Sydney, additional, Morgan, Ryan, additional, Onalaja, Oluwatobi, additional, Otero, Louis, additional, Padhye, Shivani, additional, Rainey, Emily, additional, Rasul, Fareed, additional, Robichaux, Kobe, additional, Rodier, Alexandra, additional, Schlosser, Sydni, additional, Sciacchitano, Ava, additional, Stewart, Emma, additional, Thakkar, Rajvi, additional, and Heller, Danielle M, additional

Published
2024
Full Text
View/download PDF

3. Annotation of Secretariat and Hydrus, two DJ cluster phages isolated on Gordonia rubripertincta

Author

Bland, Jackson, primary, Miller, Sydney, additional, Algarin-Martinez, Elijah D., additional, Biggs, Abbigail M., additional, Cavasini, Maria E. D., additional, Chase, Michael A., additional, Coleman, Caitlyn, additional, Correa, Victoria, additional, Danielson, Don F., additional, Dean, Wynter R., additional, French, Joseph L., additional, Horne, Mae E., additional, Macumber, Breanna M., additional, Martini, Francesca Kristal, additional, Mazzei, Sydney G., additional, McGarrah, Coen E. E., additional, Odegaard, Oslow, additional, Parameswaran, Indu S., additional, Quarterman, Corrisa, additional, Rand, Taylor M., additional, Ruiz-Houston, Kira M., additional, Sciacchitano, Ava R., additional, Seidensticker, Nina S., additional, Soltys, Anna, additional, Terron-Osorio, Adrian E., additional, Todd, Allyson L., additional, Wood, Audrey R., additional, Ungrey, Maxwell D., additional, and Pollenz, Richard S., additional

Published
2023
Full Text
View/download PDF

4. A genome-wide cytotoxicity screen of Cluster F1 mycobacteriophage Girr reveals novel inhibitors ofMycobacterium smegmatisgrowth

Author

Pollenz, Richard S, primary, Barhnhill, Kaylee, additional, Biggs, Abbigail, additional, Bland, Jackson, additional, Carter, Victoria, additional, Chase, Michael, additional, Clark, Hayley, additional, Coleman, Caitlyn, additional, Daffner, Marshall, additional, Deam, Caitlyn, additional, Finocchiaro, Alyssa, additional, Franco, Vanessa, additional, Fuller, Thomas, additional, Pinera, Juan Gallardo, additional, Horne, Mae, additional, Howard, Zoe, additional, Kanahan, Olivia, additional, Miklaszewski, Chris, additional, Miller, Sydney, additional, Morgan, Ryan, additional, Onalaja, Oluwatobi, additional, Otero, Louis, additional, Padhye, Shivani, additional, Rainey, Emily, additional, Rasul, Fareed, additional, Robichaux, Kobe, additional, Rodier, Alexandra, additional, Schlosser, Sydni, additional, Sciacchitano, Ava, additional, Stewart, Emma, additional, Thakkar, Rajvi, additional, and Heller, Danielle, additional

Published
2023
Full Text
View/download PDF

6. Genome Sequence of VanLee, a Singleton Actinobacteriophage That Infects Multiple Gordonia Strains

Author

Franco, Vanessa, primary, Barnhill, Kaylee, additional, Biggs, Abbigail, additional, Bland, Jackson, additional, Choudhary, Harris, additional, Crogan, Trey, additional, Finocchiaro, Alyssa, additional, Fuller, Thomas, additional, Hanwacker, Christopher, additional, Howard, Zoe, additional, Iqbal, Mohammed, additional, Mathew, Ann, additional, Miller, Sydney, additional, Padhye, Shivani, additional, Rainey, Emily, additional, Rodriguez, Arianna, additional, Stewart, Emma, additional, Chase, Michael, additional, Otero, Louis, additional, and Pollenz, Richard S., additional

Published
2021
Full Text
View/download PDF

7. A genome-wide cytotoxicity screen of cluster F1 mycobacteriophage Girr reveals novel inhibitors of Mycobacterium smegmatis growth.

Author

Pollenz RS, Barnhill K, Biggs A, Bland J, Carter V, Chase M, Clark H, Coleman C, Daffner M, Deam C, Finocchiaro A, Franco V, Fuller T, Pinera JG, Horne M, Howard Z, Kanahan O, Miklaszewski C, Miller S, Morgan R, Onalaja O, Otero L, Padhye S, Rainey E, Rasul F, Robichaux K, Rodier A, Schlosser S, Sciacchitano A, Stewart E, Thakkar R, and Heller DM

Subjects
Viral Proteins genetics, Viral Proteins metabolism, Mycobacterium smegmatis virology, Mycobacteriophages genetics, Genome, Viral
Abstract

Over the past decade, thousands of bacteriophage genomes have been sequenced and annotated. A striking observation from this work is that known structural features and functions cannot be assigned for >65% of the encoded proteins. One approach to begin experimentally elucidating the function of these uncharacterized gene products is genome-wide screening to identify phage genes that confer phenotypes of interest like inhibition of host growth. This study describes the results of a screen evaluating the effects of overexpressing each gene encoded by the temperate Cluster F1 mycobacteriophage Girr on the growth of the host bacterium Mycobacterium smegmatis. Overexpression of 29 of the 102 Girr genes (~28% of the genome) resulted in mild to severe cytotoxicity. Of the 29 toxic genes described, 12 have no known function and are predominately small proteins of <125 amino acids. Overexpression of the majority of these 12 cytotoxic no known functions proteins resulted in moderate to severe growth reduction and represent novel antimicrobial products. The remaining 17 toxic genes have predicted functions, encoding products involved in phage structure, DNA replication/modification, DNA binding/gene regulation, or other enzymatic activity. Comparison of this dataset with prior genome-wide cytotoxicity screens of mycobacteriophages Waterfoul and Hammy reveals some common functional themes, though several of the predicted Girr functions associated with cytotoxicity in our report, including genes involved in lysogeny, have not been described previously. This study, completed as part of the HHMI-supported SEA-GENES project, highlights the power of parallel, genome-wide overexpression screens to identify novel interactions between phages and their hosts., Competing Interests: Conflicts of interest The authors declare no conflict of interest., (© The Author(s) 2024. Published by Oxford University Press on behalf of The Genetics Society of America.)

Published
2024
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results