Search

Your search keyword '"Blais, Burton W."' showing total 201 results
Start Over Author "Blais, Burton W."
201 results on '"Blais, Burton W."'

15. Polymer Micropillar Arrays for Colorimetric DNA Detection

Author

Geissler, Matthias, primary, Malic, Lidija, additional, Morton, Keith J., additional, Clime, Liviu, additional, Daoud, Jamal, additional, Hernández-Castro, Javier A., additional, Corneau, Nathalie, additional, Blais, Burton W., additional, and Veres, Teodor, additional

Published
2020
Full Text
View/download PDF

17. Centrifugal microfluidic lab-on-a-chip system with automated sample lysis, DNA amplification and microarray hybridization for identification of enterohemorrhagic Escherichia coli culture isolates

Author

Geissler, Matthias, primary, Brassard, Daniel, additional, Clime, Liviu, additional, Pilar, Ana Victoria C., additional, Malic, Lidija, additional, Daoud, Jamal, additional, Barrère, Virginie, additional, Luebbert, Christian, additional, Blais, Burton W., additional, Corneau, Nathalie, additional, and Veres, Teodor, additional

Published
2020
Full Text
View/download PDF

22. A nucleic acid sequence-based amplification system for detection of Listeria monocytogenes hlyA sequences

Author

Blais, Burton W., Turner, Geoff, Sooknanan, Roy, and Malek, Lawrence T.

Subjects
Gene amplification -- Observations, Nucleotide sequence -- Research, Messenger RNA -- Observations, Dairy products -- Analysis, Listeria monocytogenes -- Research, Hemolysis and hemolysins -- Observations, Biological sciences
Abstract

A nucleic acid sequence-based amplification system is a sensitive, specific and reliable method for detection of gene mRNA sequences in Listeria monocytogenes strains and isolates in a variety of dairy and egg products. The system detects low numbers of L. monocytogenes cells inoculated into the food samples after a 48 hr enrichment period in modified listeria enrichment broth. The system reliably detects nucleic acids in samples, and gives positive results in the assays of numerous listeria strains and isolates from the food samples.

Published
1997

23. Transcriptional enhancement of the Listeria monocytogenes PCR and simple immunoenzymatic assay of the product using anti-RNA:DNA antibodies

Author

Blais, Burton W.

Subjects
Listeria monocytogenes -- Research, Polymerase chain reaction -- Usage, Immunoenzyme technique -- Usage, Biological sciences
Abstract

A technique to improve the responsiveness of a Listeria monocytogenes PCR detection system through in vitro transcription of amplicons, integrating bacteriophage T7 RNA polymerase promoter sequences in a priming oligonucleotide, did not react with other Listeria or non-Listeria species. Immunoenzymatic assay of the RNA-DNA hybrid with an anti-RNA-DNA hybrid antibody, following hybridization with a DNA probe immobilized in the wells of a microtiter plate, facilitated identification of the transcript.

Published
1994

26. A simple RNA probe system for analysis of Listeria monocytogenes polymerase chain reaction products

Author

Blais, Burton W. and Phillippe, Lucille M.

Subjects
Listeria monocytogenes -- Genetic aspects, Polymerase chain reaction -- Analysis, RNA -- Synthesis, Bacteriophages -- Genetic aspects, Biological sciences
Abstract

A simple and cheap technique for the in vitro synthesis of an RNA probe employed a bacteriophage T7 RNA poylmerase and a polymerase chain reaction (PCR)-generated template from hlyA, which used bacteriophage T7 promoter sequences. Transcription templates have been used to develop RNA for gene sequencing and in the isothermal magnification of nucleic acids. The new method yielded high levels of RNA which was hybridized with hlyA PCR derivatives on a membrane. This led to the formation of RNA-DNA hybrids, which were identified by an immunoenzymatic assay with an anti-RNA-DNA hybrid antibody.

Published
1993

29. Genomic Markers for Quaternary Ammonium Compound Resistance as a Persistence Indicator for Listeria monocytogenesContamination in Food Manufacturing Environments

Author

Cooper, Ashley L., Carrillo, Catherine D., DeschêNes, Mylène, and Blais, Burton W.

Abstract

Persistent contamination of food manufacturing environments by Listeria monocytogenesis an important public health risk, because such contamination events defy standard sanitization protocols, for example, the application of quaternary ammonium compounds such as benzalkonium chloride (BC), providing a source for prolonged dissemination of the bacteria in food products. We performed whole genome sequencing analyses of 1,279 well-characterized L. monocytogenesisolates from various foods and food manufacturing environments and identified the bcrABCgene cassette associated with BC resistance in 531 (41.5%) isolates. The bcrABCcassette was significantly associated with L. monocytogenesisolates belonging to clonal complex (CC) 321, CC155, CC204, and CC199, which are among the 10 most prevalent genotypes recovered from foods and food production environments. All but 1 of the 177 CC321 isolates harbored the bcrABCcassette. In addition, 384 (38.6%) of the 994 isolates recovered from foods representing 67 different CCs and 119 (59.2%) of isolates from food manufacturing environmental samples representing 26 different CCs were found to harbor the intact bcrABCcassette. A representative set of 69 isolates with and without bcrABCwas assayed for the ability to grow in the presence of BC, and 34 of 35 isolates harboring the bcrABCcassette exhibited MICs of ≥10 μg/mL BC. Determination of bcrABCin isolates could be achieved using both PCR and whole genome sequencing techniques, providing food testing laboratories with options for the characterization of isolates. The ability to determine markers of quaternary ammonium compound resistance such as bcrABCand epidemiologic lineage may provide risk managers with a tool to assess the potential for persistent contamination of the food manufacturing environment and the need for more targeted surveillance to ensure the efficacy of mitigation actions.

Published
2021
Full Text
View/download PDF

32. Overcoming microbial inhibition of S. sonneithrough the exploitation of genomically-predicted antibiotic resistance profiles for the development of food enrichment media

Author

Yao, Lang, Cooper, Ashley, Gill, Alex, Koziol, Adam, Wong, Alex, Blais, Burton W., and Carrillo, Catherine D.

Abstract

•Resistance to TMP, STR or SUL is predicted for 97.6% of published S. sonneigenomes.•AMR gene detection correlates with phenotypic resistance to these antimicrobials.•Bacteriocin-producing E. colirepresses the growth of S. sonneiin co-cultures.•Antimicrobials can be used for selective enrichment of resistant S. sonnei.•Genomically-informed selective enrichment will aid food safety investigations.

Published
2024
Full Text
View/download PDF

33. Strain-specific Recovery of S. sonneifrom Artificially Contaminated Baby Carrots: Enhancing Food-safety Investigations with a Customized ShigellaDetection Method Based on Genomically predicted Antibiotic Resistance Traits

Author

Yao, Lang, Cooper, Ashley, Ho-Fung Lau, Calvin, Wong, Alex, Blais, Burton W., and Carrillo, Catherine D.

Abstract

•Bacteriocin-producing E. coliimpede the growth of S. sonneiin food enrichment culture.•Trimethoprim in mTSB improved recovery of S. sonnei-spiked carrots.•Streptomycin in SB did not repress the growth of interfering E. coliin carrots.•Challenging microbiota can be overcome with the addition of targeted antibiotics.•Genomically informed Shigellaselective enrichment will aid food safety investigations.

Published
2024
Full Text
View/download PDF

35. Genomically Informed Strain-Specific Recovery of Shiga Toxin–Producing Escherichia coliduring Foodborne Illness Outbreak Investigations

Author

Blais, Burton W., Tapp, Kyle, Dixon, Martine, and Carrillo, Catherine D.

Abstract

Next-generation sequencing plays an important role in the characterization of clinical bacterial isolates for source attribution purposes during investigations of foodborne illness outbreaks. Once an illness cluster and a suspect food vehicle have been identified, food testing is initiated for confirmation and to determine the scope of a contamination event so that the implicated lots may be removed from the marketplace. For biochemically diverse families of pathogens such as Shiga toxin–producing Escherichia coli(STEC), the ability to detect specific strains may be hampered by the lack of a universal selective enrichment approach for their recovery against high levels of background microbiota. The availability of whole genome sequence data for a given outbreak STEC strain prior to commencement of food testing may provide food microbiologists an opportunity to customize selective enrichment techniques favoring the recovery of the outbreak strain. Here we demonstrate the advantages of using the publicly available ResFinder tool in the analysis of STEC model strains belonging to serotypes O111 and O157 to determine antimicrobial resistance traits that can be used in formulating strain-specific enrichment media to enhance recovery of these strains from microbiologically complex food samples. The improved recovery from ground beef of model STEC strains with various antimicrobial resistance profiles was demonstrated using three classes of antibiotics as selective agents, suggesting the universal applicability of this new approach in supporting foodborne illness investigations.

Published
2019
Full Text
View/download PDF

36. Microfluidic Integration of a Cloth-Based Hybridization Array System (CHAS) for Rapid, Colorimetric Detection of Enterohemorrhagic Escherichia coli (EHEC) Using an Articulated, Centrifugal Platform

Author

Geissler, Matthias, primary, Clime, Liviu, additional, Hoa, Xuyen D., additional, Morton, Keith J., additional, Hébert, Harold, additional, Poncelet, Lucas, additional, Mounier, Maxence, additional, Deschênes, Mylène, additional, Gauthier, Martine E., additional, Huszczynski, George, additional, Corneau, Nathalie, additional, Blais, Burton W., additional, and Veres, Teodor, additional

Published
2015
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results