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Background: In cancer-related diseases, early detection and control of disease progression are very important for successful treatment. Breast cancer is a significant problem due to its high mortality rate in the female population worldwide. By the early diagnosis of breast cancer, the 5-year survival rate reaches 93 to 98%. In this study, to identify breast cancer biomarkers, we construct new protein-protein interaction (PPI) and miRNAs-mRNAs networks by analyzing upregulated and downregulated genes in breast cancer patients.Method: In this in silico study, two gene expression profile datasets, with the accession numbers GSE42568 and GSE154255, were downloaded from the GEO database. GEO2R was used to obtain differentially expressed mRNA (DEMs) and miRNAs (DEMIs) based on |logFC|>2 and adjusted P-value

Abstract Antibody-drug conjugates (ADCs) are an emerging strategy in cancer therapy, enhancing precision and efficacy by linking targeted antibodies to potent cytotoxic agents. This study introduces a novel ADC that combines ribonuclease A (RNase A) with cetuximab (Cet), an anti-EGFR monoclonal antibody, through a polyethylene glycol (PEG) linker (RN-PEG-Cet), aimed to induce apoptosis in KRAS mutant colorectal cancer (CRC) via a ROS-mediated pathway. RN-PEG-Cet was successfully synthesized and characterized for its physicochemical properties, retaining full enzymatic activity in RNA degradation and high binding affinity to EGFR. In KRAS mutant SW-480 cells, RN-PEG-Cet significantly reduced cell viability at lower doses, with an IC50 of 11.7 µg/mL at 72 h. Compared to free Cet, RN-PEG-Cet demonstrated a ~ 2-fold increase in apoptosis and a ~ 3.5-fold increase in ROS production. The conjugate also disrupted the Nrf2/Keap1 pathway, with a significant upregulation of Keap1 (FC = 3.7, p ≤ 0.01) and downregulation of Nrf2 (FC = 3.3, p

Abstract Despite noticeable benefits of appropriate planting date to manage different crop diseases, efficiency of this sustainable agricultural practice in integrated crop management is little understood. It is also highly desired to study whether planting at appropriate date can improve durability of genetic resistance and thus, improve productivity. Based on meta-analysis of 318 experimental observations, pathogen and resistance factors affected not only disease control efficiency but also yield improvement. Greater control efficiencies were obtained for Phacopsora pachyrhizi, Fusarium spp., Blumeria graminis, Sclerotinia sclerotiorum, Tilletia spp. and streak mosaic virus. Yield improvements for appropriate planting dates ranged within 52–213% across different pathosystems. Greater disease control efficiencies were detected for susceptible crops compared to resistant crops planted at appropriate dates. Greater yield was detected in susceptible than resistant crops. Principal component analysis signified relationships between control efficiency, genetic resistance, planting date and yield. Planting date could be considered in developing sustainable cropping systems.

Abstract Glycogen storage disorders (GSD) GSD-IX are characterized by deficiencies in muscular and/or hepatic phosphorylase enzymes. GSD type IX za is an X-linked disorder, while IXb and IXc are autosomal recessive disorders resulting from pathogenic variants in the genes encoding the Phosphorylase b Kinase regulatory subunit alpha (PHKA), beta (PHKB), and gamma (PHKG), respectively. Despite progress in understanding these diseases, there are still unclear questions regarding their clinical manifestations, genetic variations, and the relationship between genotype and phenotype. Therefore, this review focuses on variants of GSD IX subtypes and all clinical findings to establish a genotype–phenotype relationship as well as highlighting the wide spectrum of disease-causing variants. Such information is beneficial for the establishment of a privileged mutation screening process in a specific region or ethnic group. Diagnosis is based on clinical manifestations and laboratory test results, but molecular analysis is often necessary to distinguish the various forms with similar presentations.

Despite marked improvements in the health index in recent years, the right to health of millions of people is under increasing threat. The right to health is everyone's right to enjoy the highest attainable physical and mental health standards. It is closely linked to the realization of other human rights. The right to health encompasses access not only to health services but also to economic stability, quality education, employment, housing, suitable living facilities, and other aspects that contribute to health. To overcome these challenges, the theme of World Health Day 2024 has been selected as "My Health, My Right." This year's theme was selected to champion the right of everyone, everywhere, to have access to quality health services, education, and information, as well as healthy drinking water, clean air, good nutrition, quality housing, good working and environmental conditions.

Abstract Objective Methylmalonic acidemia (MMAs) is known as a severe, complex, and lethal disorder of methylmalonate and cobalamin. The patients with MMA may have developmental, neurological, and metabolic disorders such as liver disease. Here, we aim to evaluate 6 Iranian patients suspected to MMA disorder. Study design We will provide genetic results, biochemical analysis and treatment for these patients. Liquid chromatography-tandem mass spectrometry (LC–MS/MS) and variant screening in probands by whole exome sequencing (WES) were performed. Results A total of six homozygous variants were identified, including five previously identified variants and one novel variant, in the two MMA-causing genes as follows: c.577G > C, c.290 + 69G > T, c.662T > A, c.290 + 69G > T of MMAB, and c.100dupA, c.394 C > T of MMACHC. Sanger sequencing confirmed the identified variants. Additionally, metabolomics data analysis reliably identified elevated C3 and MMA levels, as well as abnormalities in the amino acid profile, indicating the presence of pathogenic variants. Conclusions Our findings expand the global spectrum of genotypes in MMA. While WES, combined with metabolomics and biochemical analysis, offers valuable insights for accurate diagnosis and subtyping of MMA, it is most beneficial in complex cases where clinical findings are unclear.

Advanced material sciences and technologies can help to address environmental challenges in order to achieve sustainable development goals by developing innovative materials capable of mitigating energy consumption in treatment systems. In this study, an innovative electrocoagulation unit for algae removal was optimized, and the effects of various variables, including novel cathode materials (i.e., graphite and reduced graphene oxide nanoparticles), on treatment efficiency and energy consumption were evaluated. Reduced graphene oxide nanoparticles were synthesized and then immobilized on the graphite cathode surface with the modified Hummer's method. Stabilization of nanoparticles was achieved with polytetrafluoroethylene. The use of the reduced graphene oxide nanoparticles-coated cathode led to a significant decrease (42.93%) in energy consumption, compared to the case with an aluminum cathode. In the optimum conditions (a current density of 3 mA/cm2, an electrolyte concentration of 2 g/L, an electrode surface area of 56 cm2, a processing time of 60 min, and a sedimentation time of 30 min), the novel electrocoagulation unit, equipped with an aluminum anode and a reduced graphene oxide nanoparticles-coated cathode electrode, achieved removal efficiencies of 72.69% for Chlorella species and 72.96% for turbidity.

Lung cancer (LC) is one of the most common causes of cancer-related death worldwide. It has been demonstrated that the prognosis of current drug treatments is affected by a variety of factors, including late stage, tumor recurrence, inaccessibility to appropriate treatments, and, most importantly, chemotherapy resistance. Non-coding RNAs (ncRNAs) contribute to tumor development, with some acting as tumor suppressors and others as oncogenes. The phosphoinositide 3-kinase (PI3Ks)/AKT serine/threonine kinase pathway is one of the most important common targets of ncRNAs in cancer, which is widely applied to modulate the cell cycle and a variety of biological processes, including cell growth, mobility survival, metabolic activity, and protein production. Discovering the biology of ncRNA-PI3K/AKT signaling may lead to advances in cancer diagnosis and treatment. As a result, we investigated the expression and role of PI3K/AKT-related ncRNAs in clinical characteristics of lung cancer, as well as their functions as potential biomarkers in lung cancer diagnosis, prognosis, and treatment.

Abstract Background Zellweger spectrum disorders (ZSDs) are a group of peroxisome biogenesis disorders (PBDs) with different variants in the PEX genes. The main biochemical marker for screening peroxisomal disorders is very long-chain fatty acids (VLCFAs). The study reveals a rare case of PBD in the Zellweger spectrum in which she had normal plasma VLCFA levels. Case presentation Here, we report a 10-year-old girl with neurodevelopmental delay, facial dysmorphism, and hearing impairment. A brain magnetic resonance imaging scan was done to determine the reason for the seizures and neurodevelopmental delay. MRI images showed a mild widening in sulci especially in frontal lobes and sylvian fissures with pachygyria in the perisylvian regions. Biochemical analysis was done to detect ZSD. However, plasma VLCFA levels were normal. The diagnosis was made using whole-exome sequencing (WES). A homozygous variant of uncertain significance (VUS) in PEX6 NM_000287.4: c.1992G > C (p. Glu664Asp) was identified which has been confirmed through Sanger sequencing in probands and her parents. Conclusions According to the case report, plasma VLCFA levels can be normal in patients with PBDs in the Zellweger spectrum. Furthermore, we could re-classify the c.1992G > C variant in the PEX6 gene from VUS to likely pathogenic based on clinical manifestations including facial dysmorphism, and hearing impairment.

Abstract Background Allogeneic hematopoietic stem cell transplantation is a definitive cure for eligible patients with thalassemia major, and calcineurin inhibitors are essential for preventing graft-versus-host disease. Although invaluable, there are few reports of life-threatening hypersensitivity reactions associated with calcineurin inhibitors. These reactions are generally rare but seem to be more prevalent among patients with thalassemia. Case presentation Herein, we retrospectively report four cases of patients with thalassemia major who developed hypersensitivity reactions to parenteral cyclosporine. The cases include one 19-year-old Caucasian female and three Caucasian males, aged 17, 10, and 20 years, respectively. The patients exhibited symptoms of varying severity, necessitating different management strategies. The reactions occurred either immediately or within a few minutes after the onset of cyclosporine infusion and were often worsened by rechallenge. In all cases, cyclosporine was eventually replaced with tacrolimus or sirolimus. A comprehensive literature review was conducted to investigate the basis of severe immunoglobulin E-mediated hypersensitivity reactions to calcineurin inhibitors in patients with thalassemia major undergoing hematopoietic stem cell transplantation. Conclusions Several immunogenic factors may potentially increase the susceptibility of these patients to hypersensitivity reactions to Cremophor-containing medications. While severe reactions to calcineurin inhibitors remain rare, clinicians should be aware of the potential for serious adverse events in patients with thalassemia. Graphical Abstract

Abstract Objectives To assess whether the combination of scapular-focused training and mulligan mobilization (SFTMM) improves pain and proprioception compared to scapular-focused training (SFT) and a control group in female rock climbers with shoulder impingement syndrome (SIS). Design Three-arm randomized controlled trial (RCT). Setting Outpatient setting. Subjects Individuals were randomly assigned to SFTMM, SFT alone, and control group. Interventions 8 weeks of SFTMM and SFT. Main measures Outcome measures were pain and proprioception. Results The results revealed significant differences in pain scores and proprioception among female rock climbers with SIS who participated in SFTMM, SFT, and a control group (F(2, 32) = 81.01, p = 0.001, η2 = 0.83 for pain scores; F(2, 32) = 178.2, p = 0.001, η2 = 0.91 for proprioception scores). Post-hoc tests via the Bonferroni test indicated that both SFTMM and SFT significantly reduced pain levels (p = 0.001) and improved proprioception levels (p = 0.001) compared with the control group. There was no significant difference in pain scores and proprioception between the SFTMM group and the SFT group (p > 0.05). Conclusions In conclusion, the study indicates that SFTMM significantly reduces pain and improves proprioception in female rock climbers with SIS, as shown by notable changes compared to the control group. However, no statistically significant difference was found between the SFTMM (combined intervention) and SFT alone. Therefore, while the incorporation of SFT and MM shows promise; further research is needed to fully understand its long-term benefits and clinical implications. Ethical Code Registration The study was approved at Ethics.research.ac.ir, code: IR.SSRC.REC.1402.170 on 2023-10-22.

Abstract Increasing evidence suggests an essential function for autophagy in unconventional protein secretion (UPS). However, despite its relevance for the secretion of aggregate-prone proteins, the mechanisms of secretory autophagy in neurons have remained elusive. Here we show that the lower motoneuron disease-associated guanine exchange factor Plekhg5 drives the UPS of Sod1. Mechanistically, Sod1 is sequestered into autophagosomal carriers, which subsequently fuse with secretory lysosomal-related organelles (LROs). Exocytosis of LROs to release Sod1 into the extracellular milieu requires the activation of the small GTPase Rab26 by Plekhg5. Deletion of Plekhg5 in mice leads to the accumulation of Sod1 in LROs at swollen presynaptic sites. A reduced secretion of toxic ALS-linked SOD1G93A following deletion of Plekhg5 in SOD1G93A mice accelerated disease onset while prolonging survival due to an attenuated microglia activation. Using human iPSC-derived motoneurons we show that reduced levels of PLEKHG5 cause an impaired secretion of ALS-linked SOD1. Our findings highlight an unexpected pathophysiological mechanism that converges two motoneuron disease-associated proteins into a common pathway.

Abstract Introduction The Fear of Pain Questionnaire (FOPQ) is a self-report tool designed to measure an individual’s fear of pain (FOP). While the Persian version of the FOPQ (FOPQ-P) has been developed, its validity and reliability have not yet been assessed in the Iranian context. This study aims to evaluate the psychometric properties of the FOPQ-P among Iranian patients after surgery. Methods A methodological study was conducted in 2023 involving 400 post-surgery patients selected with a convenience sampling. The FOPQ was translated into Persian, and its psychometric properties were analyzed using network analysis, exploratory factor analysis (EFA), confirmatory factor analysis (CFA), as well as assessments of convergent and discriminant validity. Internal consistency was measured using Cronbach’s alpha, McDonald’s Omega, average inter-item correlation coefficient, Composite Reliability, and Maximal Reliability. Results The EFA results with Promax and Kaiser Normalization rotation identified two factors that explained 54.32% of the variance, comprising seven items. The CFA confirmed the model’s validity. Both convergent and discriminant validity were established. The reliability analyses showed that Cronbach’s alpha, McDonald’s omega, composite reliability, and MaxR for all constructs were above 0.7. Additionally, the average inter-item correlation coefficient was greater than 0.5, indicating strong internal consistency and construct reliability. Conclusion The findings suggest that the FOPQ-P possesses a valid structure and was acceptable reliability in patients cultural context of Iran post-surgery, making it a suitable instrument for measuring fear of pain in this population.

Abstract Clinical bleeding events are reported here from 773 patients with B‐cell malignancies receiving pirtobrutinib monotherapy from the phase 1/2 BRUIN study (ClinicalTrials.gov identifier: NCT03740529), either in the presence or absence of antithrombotic therapy (antithrombotic exposed [AT‐E], n = 216; antithrombotic nonexposed [AT‐NE], n = 557). Among the AT‐E cohort, 51.9% received platelet aggregation inhibitors, 36.6% received direct factor Xa inhibitors, 18.5% received heparins, 5.6% received salicylic acid for indications other than platelet aggregation inhibition, and 2.3% received thrombolytics. Warfarin was not permitted. Any‐grade bleeding/bruising events occurred in 97 patients (44.9%; 95% confidence interval [CI], 38.3–51.5) in the AT‐E cohort and 181 patients (32.5%; 95% CI, 28.6–36.4) in the AT‐NE cohort. Most bleeding/bruising events in both cohorts began within the first 6 months of treatment (AT‐E: 65.4%; AT‐NE: 72.5%). Contusion was the most common bleeding/bruising event in both cohorts (AT‐E: 22.7%; AT‐NE: 18.1%). Grade ≥3 bleeding/bruising events were reported in six patients (2.8%) in the AT‐E cohort and 11 patients (2.0%) in the AT‐NE cohort. Bleeding/bruising events requiring or prolonging hospitalization were reported in 2.3% and 1.6% of patients in the AT‐E and AT‐NE cohorts, respectively. No bleeding/bruising events led to pirtobrutinib dose reduction or permanent discontinuation in the AT‐E cohort, and one patient (0.2%) in the AT‐NE cohort experienced an event requiring dose reduction. These data support the safety of pirtobrutinib in patients requiring antithrombotic therapies.

Abstract In this study, improvement of the electro-Fenton process using Fe-MIL-88B along with the innovation in the reactor with the simultaneous rotation of the cathodes and anodes was carried out to remove Acid Blue 25. For this purpose, the Fe-MIL-88B nanocatalyst was synthesised by the thermal solvent method and was characterised by FT-IR, EDAX, XRD, and FESEM. For the experiments, an electrochemical cell with a useful volume of 1 L and rotating cathodes and anodes were used and nanoparticles were added to the system as a slurry. To determine the appropriate values of the effective parameters, the OFAT method was used. According to the results, 0.3 g/L Fe-MIL-88B, pH equal to 3, dye concentration of 75 mg/L, a current intensity of 0.228 A, and a rotation velocity of 100 rpm were chosen as suitable values for the process. In optimal conditions, the dye removal efficiency reached to 92.3% after 90 min, which resulted in a 14.5% improvement in the dye removal efficiency compared to the conventional process under the same conditions. Also, after 90 min, the removal efficiencies of 77.08 and 63.63% were obtained for COD and TOC, respectively. The results indicated acceptable decomposition of the effluent into less hazardous compounds.

Abstract Background Simulation-based training courses have been developed and widely accepted.The use of simulators can prevent bitter consequences that may sometimes put human lives in danger. Increasing the skill of nursing students in dealing with trauma patients is of great importance. The aim of this study is to evaluate the effectiveness of the simulation-based trauma training program for the management of trauma patients in nursing students. Method It is an observational approach that focuses on improving the learning of trauma care skills. For this study, 6th and 8th semester nursing students were selected by appropriate sampling method. Previously, these students completed the trauma training course without simulation. Training of trauma skills was done using multi-purpose trauma mollage And we subsequently analyzed the effectiveness of the trauma simulation training program using the OSCE test. In the inferential statistics section, after examining the data distribution (Kolmogrove-Smirnov and Shapiro-Wilk tests), the Wilcoxon test was used to measure significance. SPSS 22 software was used. Results The results indicated that the lowest mean score obtained based on a scale of zero to one hundred was for the movement limitation of the injured long bone and the highest was in the skill of oral-nasal intubation and ventilation. The results of the paired t-test showed that clinical skills for dealing with trauma-affected individuals in undergraduate nursingafter simulation training was significantly higher . Conclusion The implementation of the simulation is accompanied by an increase in the skills of the participants, which leads to the application of the acquired knowledge in real-life scenarios and positive changes. In the learning of the participants, the evaluation of conventional trauma training in Iran, like other countries, shows the need for specialized training through simulation. To ensure the continued effectiveness of simulation-based trauma training courses, it is recommended that administrators and policymakers encourage regular faculty participation in the program. Long term effects of trauma simulators training in nursing student requires further study.

Abstract Background Cholera is a diarrheal disease recognized for being caused by toxin-producing Vibrio (V.) cholerae. This study aims to assess the vibriocidal and immunomodulatory properties of derived cell-free supernatants (CFSs) of Bifidobacterium (B.) bifidum and Lactobacillus (L.) acidophilus encapsulated in chitosan nanoparticles (CFSb-CsNPs and CFSa-CsNPs) against clinical multi-drug resistance (MDR) isolates of V. cholerae O1 El Tor. Methods We synthesized CFSb-CsNPs and CFSa-CsNPs using the ionic gelation technique. The newly nanostructures were characterized for size, surface zeta potential, morphology, encapsulation efficacy (EE), stability in different pH values and temperatures, release profile, and in vitro cytotoxicity. The antimicrobial and antibiofilm effects of the obtained nanocomposites on clinical MDR isolates (N = 5) of V. cholerae E1 Tor O1 were investigated by microbroth dilution assay and crystal violet staining, respectively. We conducted quantitative real-time PCR (qRT-PCR) to analyze the relative gene expressions of Bap, Rbmc, CTXAB, and TCP in response to CFSb-CsNPs and CFSa-CsNPs. Additionally, the immunomodulatory effects of formulated structures on the expression of TLR2 and TLR4 genes in human colorectal adenocarcinoma cells (Caco-2) were studied. Results Nano-characterization analyses indicated that CFSb-CsNPs and CFSa-CsNPs exhibit spherical shapes under scanning electron microscopy (SEM) imaging, with mean diameters of 98.16 ± 0.763 nm and 83.90 ± 0.854 nm, respectively. Both types of nanoparticles possess positive surface charges. The EE% of CFSb-CsNPs was 77 ± 4.28%, whereas that of CFSa-CsNPs was 62.5 ± 7.33%. Chitosan (Cs) encapsulation leads to increased stability of CFSs in simulated pH conditions of the gastrointestinal tract in which the release rates for CFSb-CsNPs and CFSa-CsNPs were reached at 58.00 ± 1.24% and 55.01 ± 1.73%, respectively at pH = 7.4. The synergistic vibriocidal effects observed from the co-administration of both CFSb-CsNPs and CFSa-CsNPs, as evidenced by a fractional inhibitory concentration (FIC) index of 0.57, resulting in a significantly lower MIC of 2.5 ± 0.05 mg/mL (p

Abstract Background Despite the severe impact of COVID-19 on cancer patients, data on COVID-19 outcomes in cancer patients from low- and middle-income countries is limited. We conducted a large study about the mortality rate of COVID-19 in cancer patients in Iran. Methods We analyzed data from 1,079 cancer (average age: 58.2 years) and 5,514 non-cancer patients (average age: 57.2 years) who were admitted for COVID-19 in two referral hospitals between March 2019 and August 2021. Patients were followed up until death or 31st August 2021. Multiple logistic regression models estimated the odds ratio (OR) and 95% confidence intervals (CI) of factors associated with ICU admission and intubation. The Cox regression model estimated hazard ratios (HRs) and 95% CI of factors associated with hospital and post-discharge 60-day mortalities. Results The cancer patients had higher ICU admission (OR = 1.65, 95% CI: 1.42–1.91; P-value 0.03) and intubation (OR = 3.13, 95% CI = 2.63–3.73, P-value

Abstract Background Basal cell carcinoma is the most prevalent skin cancer, most characterized by local aggressiveness but with low metastatic potential, and bone metastasis is quite heterogeneous, thus the incidence profile is variable size from 0.0028% to 0.5%. We have this patient with an unusual example of basal cell carcinoma with bone metastases to add to the scarce report on this matter. Case description Here we document a 48-year-old Persian man with a background of being exposed to the sun for a long time. He was diagnosed with an ulcer on the cheek, which was clinically characterized and further confirmed by biopsy as morpheaform basal cell carcinoma. Following the first round of excision, multiple relapses eventually metastasized to the bone. The latter was found on follow-up radiologic scans. This case is characterized by the aggressive nature of the disease and the heterogeneity of basal cell carcinoma growth, thus challenging the conventional view of basal cell carcinoma behavior. Treatment included surgical excision of the primary lesion, which was treated with radiotherapy afterward. However, the skeleton improved slowly during follow-up, and palliative care was eventually pursued to control symptoms and improve quality of life. Conclusions This was a rare case of basal cell carcinoma metastasis to non-bone organs, which reminded us to consider basal cell carcinoma metastasis, especially in the case of atypical basal cell carcinoma. Therefore, risk-aware patient management is essential. Moreover, these findings highlight the role of further research into the mechanisms of basal cell carcinoma metastasis, leading to improved therapeutic strategies that may lead to potential improvements in patient outcomes.

Abstract Background Psoriatic arthritis is a chronic inflammatory condition associated with psoriasis, which affects joints. The coexistence of vasculitis, an inflammation of blood vessels, with psoriatic arthritis is rare, highlighting a complex interplay between autoimmune diseases that affect both joints and vascular structures. Case presentation We report on a 56-year-old male with a long-standing history of psoriasis and psoriatic arthritis, who presented with new-onset skin lesions and neurological symptoms. Clinical evaluations confirmed the presence of leukocytoclastic vasculitis and asymmetric sensory motor axonal neuropathy. The absence of positive results from various standard tests led to a challenging diagnostic process, ultimately pointing towards vasculitis associated with psoriatic arthritis. The patient was treated with corticosteroids and immunosuppressive therapy, leading to partial symptom improvement. Conclusions This case underlines the complex and often overlapping nature of autoimmune conditions, particularly the occurrence of vasculitis in patients with psoriatic arthritis, which is rare. It stresses the importance of considering vasculitis in patients with psoriatic arthritis who present with unexplained skin lesions and neurological symptoms. This case adds to the spectrum of psoriatic disease manifestations and suggests a need for further research to explore the underlying mechanisms and improve management strategies.

Since unfortunate events can happen to anyone at any time, it is important for everyone to understand the concept of psychological trauma and be able to identify its symptoms to prevent or treat it before it becomes too late. The character of Hamlet is traumatized from the very beginning of the play, and as the story develops, so does his trauma. Shakespeare's Hamlet is a well-known and widely performed play, making it a good option to illustrate trauma and its detrimental consequences on both the individual and others around them in real life. The study, which was founded on the constructivist paradigm, demonstrated adult psychological trauma, its symptoms, and its effects by using a phenomenological approach. Twelve people, six men and six women, over the age of eighteen (from 19 to 60), participated in this study. After an analysis of the experiences and feedback provided by the participants, it became clear that if psychological trauma is not identified and treated in a timely manner, it can negatively impact not only the victim's own life but also the lives of those close to them and even society as a whole.

Abstract This research aimed to synthesize polyvinyl fluoride membranes and coat them with tannic acid (TA) nanoparticles and polyethylene glycol (PEG) additives so that the membrane’s removal efficacy for humic acid (HA) pollutant from agricultural wastewater was investigated. Thus, six membranes with PEG:TA ratios of 0:0, 1:0, 0:1, 1:1, 4:1, and 1:4 were synthesized. Then, the membranes’ characteristics were identified by FTIR-ATR, FESEM, and AFM analysis, and HA’s particle size and zeta potential were also investigated. Based on optimizing effective parameters, the operating pressure of 1.5 bar and HA concentration of 80 ppm were selected as optimal values. The membrane with PEG:TA = 4:1, as the optimally modified membrane, had a pure water flux of 446.03 L/m2.h, effluent flux of 72.43 L/m2.h, and pollutant removal rate of 86.62% at pH = 7 after 60 min had passed. These values for the pristine membrane (PEG:TA = 0:0) were 265.64 L/m2.h, 89.39 L/m2.h, and 75.59%, respectively. The results showed that although the effluent flux was lower in the optimized modified membrane than in the pristine membrane, HA removal percentage was increased.

Background: With the increasing prevalence of infertility and its links to depression, anxiety, and stress, it is essential to compare these mental health levels between infertile men and a control group. Objective: This study aimed to compare depression, anxiety, and stress among infertile and fertile men. Also, assessing demographic factors affecting these challenges among both groups. Materials and Methods: This case-control study was conducted on 120 men at Milad Infertility Research Center, Mashhad, Iran from January 2023 to February 2023. Participants were divided into 2 groups: 60 infertile men and 60 healthy men who did not have fertility problems. Demographic information such as age, education, occupation, duration of the marriage, and duration of infertility was collected from their medical records, and they also completed the depression, anxiety, and stress scale 21 questionnaire through a telephonic interview. Finally, the findings were statistically analyzed. Results: Severe and very severe depression, anxiety, and stress were observed in 65%, 60%, and 43.4% of infertile men and 16.7%, 23.3%, and 11.7% of fertile men, respectively. Which was significantly more than the fertile group, and there was a significant relationship between depression (p ≤ 0.001), anxiety (p = 0.001), stress (p ≤ 0.001), and infertility. Also, a significant relationship was observed between the duration of infertility and depression (p = 0.031). Conclusion: Our study found infertile men had higher stress, anxiety, and depression than the control group. Limitations included phone-based data collection and the depression, anxiety, and stress scale 21 questionnaire’s screening nature. Future studies should involve larger populations and consider economic status as a variable related to mental health.

Exposure to morphine during gestation period has vital effect on infants’ neurodevelopmental growth pattern. Lutein shows protective qualities regarding neurodegeneration and the development of the brain. This study aimed to investigate effect of parental exposure to lutein on adverse effect of the morphine withdrawal syndrome on reflexive motor behavior in mice offspring. Fourteen male mice and 56 adult female mice were randomly assigned to seven groups: the control group containing male and female mice that refrained from morphine; group 2 was morphine-abstinent male mice and female mice with no prior drug exposure; group 3 including morphine-abstinent female mice and male mice without drug experience; and group 4 of drug-naïve male and female mice. Groups 5–7 were similar to groups 2–4, but drug-naïve subjects received injections of lutein (10 mg/kg). Following delivery, offspring from each group were chosen to assess behavior and reflexive motor behaviors. Also, blood samples were collected to measure serum antioxidant activity. Based on the findings, reflexive motor behaviors significantly decreased following prenatal exposure to morphine (P 0.05). Lutein administration had no effect on reflexive motor behaviors in male morphine exposed group (P > 0.05) but lutein administration significantly decreased adverse effect of the morphine on reflexive motor behaviors in female exposed group (P 0.05). Lutein administration decreased serum malondialdehyde (MDA) production and increased superoxide dismutase (SOD), glutathione peroxidase (GPx) and total antioxidant status (TAS) levels in morphine exposed mice. It seems, maternal exposure to lutein had protective role on adverse effect of the morphine on reflexive motor behaviors in offspring.

Background Mammographic breast density (MBD) is directly related to the risk of breast cancer. In vivo and in vitro studies have shown that metformin can reduce the proliferation and growth of breast cancer tissue. The aim of this study was to determine the effect of metformin on MBD in non-diabetic premenopausal women. Methods A double-blind, placebo-controlled study was performed. A total of 151 premenopausal women received 500 mg metformin tablets twice a day for 6 months, or a placebo, were included. The changes in MBD were compared between the two groups. Results Final data were evaluated based on 67 and 84 women in the metformin and placebo groups, respectively. Based on results from Ordinal Logistic Regression, the odds of achieving a higher density for the intervention group was approximately 2.33 (95% CI, 1.04 to 5.18) times that of the placebo group. Conclusions This clinical trial showed that consumption of metformin 500 mg twice daily for 6 months is associated with a higher mammographic breast density as compared to the placebo group. As metformin is used very commonly, we suggest that this medicine should be considered as a probable confounding factor when conducting studies about MBD.

[This corrects the article DOI: 10.1371/journal.pone.0308689.].

Background and purpose: Computed tomography radiostereometric analysis (CT-RSA) assesses implant micromovements using low-dose CT scans. We aimed to investigate whether CT-RSA is comparable to marker-based radiostereometric analysis (RSA) measuring early femoral head migration in cemented stems. We hypothesized that CT-RSA is comparable to marker-based RSA in evaluating femoral head subsidence. Methods: We prospectively included 31 patients undergoing cemented total hip arthroplasty (THA), of which 27 were eligible for the analysis. Femoral head migration at 1 year was measured with marker-based RSA and CT-RSA. Comparison was performed using paired analysis and Bland–Altman plots, and the intra- and interobserver reliability of CT-RSA was assessed Results: The median (interquartile range [IQR]) translation on the Y-axis measured with marker-based RSA was –0.86 mm (–1.10 to –0.37) and –0.83 mm (–1.11 to –0.48) for CT-RSA (i.e. subsidence), with a median difference of –0.03 mm (95% confidence interval [CI] –0.08 to 0.18). The minimal important difference in translation was set to 0.2 mm. This value was excluded from the CI of the differences. No statistical difference was found between marker-based RSA and CT-RSA regarding assessment of subsidence of the femoral head. The Bland–Altman plots showed good agreement between the 2 methods in measuring subsidence of the femoral head. The intra- and interobserver reliability of the CT-RSA method was excellent with intraclass correlation coefficient (ICC) = 1 (0.99–1) and ICC = 0.99 (0.99–1), respectively. Conclusion: We showed that CT-RSA was comparable to marker-based RSA in measuring femoral head subsidence. Moreover, the intra- and interobserver reliability of the CT-RSA method was excellent, suggesting that the method is assessor independent.

Background: Green space has been linked with cardiovascular (CV) health. Nature access and quality may have significant impact on CV risk factors and health. Objectives: The authors aimed to investigate the relationship between NatureScore, a composite score for natural environment exposure and quality of green spaces, with CV risk factors and atherosclerotic cardiovascular diseases (ASCVD). Methods: A cross-sectional study including one million adult patients from the Houston Methodist Learning Health System Outpatient Registry (2016-2022). NatureScore is a composite measure of natural environment exposure and quality (0-100) calculated for each patient based on residential address. NatureScores was divided into 4 categories: nature deficient/light (0-39), nature adequate (40-59), nature rich (60-79), and nature utopia (80-100). CV risk factors included hypertension, diabetes, dyslipidemia, and obesity. Results: Among 1.07 million included patients (mean age 52 years, female 59%, Hispanic 16%, Non-Hispanic Black 14%), median NatureScore was 69.4. After adjusting for neighborhood walkability, patients living in highest NatureScore neighborhoods had lower prevalence of CV risk factors (OR: 0.91, 95% CI: 0.90-0.93) and ASCVD (OR: 0.96, 95% CI: 0.93-0.98) than those in lowest NatureScore neighborhoods. A significant interaction existed between NatureScore and Walkability (P

Background and Objectives: Nosocomial pneumonia caused by multidrug-resistant gram-negative bacteria presents a significant challenge for healthcare systems, as there are limited effective treatments available. This systematic review and meta-analysis aim to investigate the outcomes of colistin plus meropenem combination therapy on nosocomial pneumonia. Materials and Methods: An exhaustive search of PubMed, Scopus, Web of Science (WOS), and Embase databases was conducted, resulting in the extraction of 5 studies for qualitative assessment and meta-analysis. The study sample included 991 patients admitted with nosocomial pneumonia. The outcomes evaluated were clinical improvement, microbiological response, mortality, Sequential Organ Failure Assessment (SOFA) score, Acute Physiology and Chronic Health Evaluation (APACHE II) score, Charlson Comorbidity Index (CCI), Clinical Pulmonary Infection Score (CPIS), C-reactive protein (CRP) levels, procalcitonin (PCT) levels, and intensive care unit (ICU) duration. Results: The results demonstrated that colistin plus meropenem combination therapy significantly improved clinical outcomes (OR = 1.37, 95% CI = 1.04-1.81, p = 0.027), reduced SOFA scores (OR = -0.28, 95% CI = -0.44 to -0.11, p = 0.001), and increased CCI scores (OR = 0.16, 95% CI = 0.02-0.29, p = 0.021) compared to other medications. However, other evaluated parameters did not show significant differences. Conclusion: This meta-analysis indicates that colistin-meropenem combination therapy is superior to other colistin-based treatments for nosocomial pneumonia in terms of clinical improvement, SOFA score reduction, and CCI score increase. Nevertheless, other variables assessed did not exhibit remarkable differences between the treatment regimens.

ABSTRACT Background Despite bilateral mastectomy (BLM) for early‐stage breast cancer (BC) showing no survival benefit and increased risk compared to breast conserving surgery, some patients still choose this treatment. This study examined whether emotion reactivity and regulation influence treatment decision making among newly diagnosed women with breast cancer. Methods Cross‐sectional survey data were analyzed as part of a larger study. Measures included the Contralateral Prophylactic Mastectomy (CPM) survey, PROMIS Anxiety scale, and Emotion Regulation Questionnaire (ERQ) Cognitive Reappraisal and Emotional Suppression subscales. Primary analysis included a logistic regression model predicting treatment choice (BLM vs. non‐BLM). Results Participants (N = 137) with unilateral BC (Stages 0–III) were divided between BLM (n = 66) versus breast conserving surgery (i.e., non‐BLM, n = 71) treatment groups. Compared to the non‐BLM group, the BLM group was younger, more likely to be partnered, and had a higher household income. Women with high levels of BC‐specific worry were 3.6 times more likely to choose BLM compared to women with low levels of worry (OR = 3.09, 95% CI: 1.07–0.8.93). Those who used cognitive reappraisal were 10% less likely to choose BLM compared to women who did not use cognitive reappraisal (OR = 0.90, 95% CI: 0.82–0.99). There were no group differences in levels of generalized anxiety (OR = 0.93, 95% CI: 0.87–0.99) or emotional suppression (OR = 1.02, 95% CI: 0.90–1.16). Conclusions Findings suggest the choice of BLM may be due, in part, to negative emotional experiences after a BC diagnosis and lesser use of reappraisal to reframe cancer‐related fears. These may be important targets of intervention to support women making BC treatment decisions.

IntroductionSo far, the psychometric properties of the Persian version of the Pain Anxiety Symptom Scale have not been assessed in Iran. Therefore, this study was conducted to determine the validity and reliability of the Persian version of the Pain Anxiety Symptom Scale among a group of Iranian patients in Amol.MethodsThis methodological study was conducted in 2023 with a sample of 400 postoperative patients from Amol, Iran, selected through convenience sampling. The dataset was divided into two groups of 200 for exploratory and confirmatory factor analyses. Construct validity was assessed using maximum likelihood exploratory factor analysis with Promax rotation, supported by Horn’s parallel analysis and network analysis to visualize item relationships. Confirmatory factor analysis, convergent and discriminant validity was performed on the second dataset. Reliability was evaluated through various statistical measures, including Cronbach’s alpha, McDonald’s omega, average inter-item correlation coefficient, composite reliability, and maximal reliability (MaxR).ResultsAmong the 400 participants, the mean age was 44.38 years (SD = 13.49), with 152 (46.1%) being women and 178 (53.9%) men. Most participants (n = 268, 81.2%) had an education level lower than a diploma, and 93 individuals (28.2%) reported a history of surgery. The results of exploratory factor analysis with Promax rotation developed two factors accounting for 66.29% of the variance comprising 15 items. Also, after necessary modifications during confirmatory factor analysis, the final model was approved. As for reliability, the Cronbach’s alpha, composite reliability, and MaxR for all constructs were greater than 0.7, demonstrating good internal consistency and construct reliability.ConclusionAccording to the results, the Persian version of the Pain Anxiety Symptom Scale has a valid structure and acceptable reliability. This scale can be used by health professionals in many ways.

Boehmite nanoparticles (BNPs) were synthesized using a simple hydrothermal approach to attain optimal crystallite size, utilizing a novel directing agent. The synthesis process employed solutions of aluminum nitrate and urea, with a particular focus on the use of an environmentally friendly surfactant/bio-directing agent, namely xanthan gum. The effect of four key parameters, including the molar concentration of xanthan gum, the ratio of urea to aluminum nitrate molar concentrations (U/N ratio), reaction time, and the molar concentration of calcium chloride, was investigated using Taguchi design. Characterization of the samples was carried out through XRD, FESEM, EDX, and BET analyses revealing the presence of the boehmite pattern in all samples. The minimum crystallite size was achieved under optimized conditions (xanthan gum molar concentration of 2 mM, U/N molar ratio of 4, the reaction time of 12 h, and CaCl2 molar concentration of 1 mM) measured 10.76 nm with a specific surface area of 63.779 m2/g.

Background: Children facing challenges such as social deficiencies during childhood may struggle in social interactions and unhealthy emotional relationships, thus increasing their vulnerability to sexual addiction. The relationship between childhood traumas and sexual addiction is influenced by various factors such as social support, mental framing, and attachment styles, highlighting the importance of thoroughly examining the impact of each of these factors on this relationship. Strong scientific evidence can guide decision-makers in implementing effective measures and policies to prevent childhood traumas and sexual addiction. Aims: The present study aimed to model the structural equations of the relationship between childhood traumas and sexual addiction, with the mediating role of perceived social support, mental framing, and attachment styles. Methods: The current research employed a descriptive correlational approach and structural equation modeling. The statistical population included all virtual students in Tehran universities during the academic year 2021-2022. The sample size was determined to be 322 individuals based on the Klein formula (2011), considering the possibility of dropouts, with 370 individuals ultimately included. Data collection utilized the Childhood Trauma Questionnaire (Bernstein et al., 2003), Sexual Addiction Screening Test (Carnes, 1988), and Adult Attachment Style Questionnaire (Collins & Read, 1990). The gathered data were analyzed using path analysis in LISREL and SPSS software. Results: The research findings indicate significant correlations, confirming the hypothesized correlation between variables for developing the structural equation model (p< 0.05). Conclusion: The relationship between childhood traumas and sexual addiction is influenced by various factors, including social support, mental framing, and attachment styles, underscoring the critical importance of thoroughly investigating the impact of each of these factors on this relationship. Strong scientific evidence can guide decision-makers in implementing effective measures and policies to prevent childhood traumas and sexual addiction.

New complexes of nickel(II) and palladium(II) were synthesized using the ferrocenyl imine ligand. This Schiff base ligand was coordinated to the metal ions through the imine N and thiolic S atoms. The synthesized complexes were characterized by FT-IR, 1H NMR, UV–Vis spectroscopy and elemental analysis. The IR spectra of complexes show characteristic of imine group. Furthermore, the 1H NMR spectra display different protons of the cyclopentadienyl groups. The binding interactions of the compounds with DNA were investigated by the electronic absorption spectroscopy, and the intrinsic binding constants (Kb) were calculated for the Schiff base ligand, nickel and palladium complexes as 2.7×104, 1.2×105 and 7.2×104, respectively. The obtained data showed that the synthesized compounds bind to DNA via intercalative binding modes. Also, viscosity was investigated in order to further explore the nature of interactions between the compounds and DNA. Accordingly, it was found that the synthesized compounds bind to DNA via intercalative binding modes.

Abstract Background There is little to no data evaluating long term usage of point of care ultrasound (POCUS) after a training intervention for medical students. The purpose of this study was to examine the impact of an intensive POCUS training program on medical student’s usage at 9-months post-program. Methods This was a prospective cross-sectional study of rising second year medical students who participated in a 2-week summer POCUS training program. Instruction consisted of 8 h of asynchronous online didactic material, 2–4 h of daily hands-on instructor-led and independent scanning, and instruction on how to teach POCUS. Students were assessed pre- and post-program, and again at 9 months post-program to evaluate POCUS usage. Results A total of 56 students participated in the program over 2 summers; 52 (92.9%) responded to the 9-month post-program survey. At 9 months, 49 (94.2%) of students taught POCUS after the program to peers or faculty. Students reported serving as a POCUS instructor in 283 subsequent teaching sessions accounting for 849 h of POCUS instruction time. Six (11.5%) students were involved in the creation of a POCUS interest group on their regional campus, 7 (13%) created a POCUS curriculum for their student interest group, and 4 (7.7%) created an opt-in co-curricular POCUS program for students at their regional campus. Three (5.8%) students did not serve as educators after the program and only one student reported not using POCUS again after the program. Conclusion After a 2-week intensive POCUS training program for medical students, the majority of students demonstrated continued involvement in POCUS learning and education at 9-month follow-up including serving as peer instructors and assisting with limitations in financial resources and trained faculty.

The aim of this study was to compare the effectiveness of mindfulness based on stress reduction and desensitization with eye movements and reprocessing on self-regulation and post-traumatic stress disorder in students. This research was a quasi-experimental study with pre-test-post-test design and sampling available. The statistical population of this study was the students of the first and second year of high school in Sarpol-e-Zahab city. After completing the Mississippi Citizenship Scale(Mississippi scale for PTSD, keane, Cadell& Taylor,1998) and the Beaufort Self-Regulatory Questionnaire( Bouffard, Boisvert, Vezeau & Larouche ,1995), 45 adolescents were randomly selected and randomly divided into three groups of 15, including two experimental groups and a control group. The first experimental group underwent desensitization with eye movements for 6 sessions of 90 minutes and the second experimental group underwent stress reduction intervention mindfulness intervention for 8 sessions of 2 hours. All three groups were followed up again 60 days later. Data were analyzed using repeated measures analysis of variance and covariance and Scheffe post hoc test. The results showed that both of the above therapies can improve self-regulation and post-traumatic stress disorder in post-test and follow-up(P

Abstract Maturity-onset diabetes of the young (MODY) is an uncommon monogenic type of diabetes mellitus. Detecting genetic variants for MODY is a necessity for precise diagnosis and treatment. The majority of MODY genetic predisposition has been documented in European populations and a lack of information is present in Iranians which leads to misdiagnosis as a consequence of defects in unknown variants. In this study, using genetic variant information of 20,002 participants from the family-based TCGS (Tehran Cardiometabolic Genetic Study) cohort, we evaluated the genetic spectrum of MODY in Iran. We concentrated on previously discovered MODY-causing genes. Genetic variants were evaluated for their pathogenicity. We discovered 6 variants that were previously reported in the ClinVar as pathogenic/likely pathogenic (P/LP) for MODY in 45 participants from 24 families (INS in 21 cases, GCK in 13, HNF1B in 8, HNF4A, HNF1A, and CEL in 1 case). One potential MODY variant with Uncertain Risk Allele in ClinVar classification was also identified, which showed complete disease penetrance (100%) in four subjects from one family. This is the first family-based study to define the genetic spectrum and estimate the prevalence of MODY in Iran. The discovered variants need to be investigated by additional studies.

The growing population and expansion of rural activities, along with changing climatic patterns and the need for water during drought periods, have led to a rise in the water demand worldwide. As a result, the construction of water storage structures such as dams has increased in recent years to meet the water needs. However, dam construction can bring significant alterations to the natural flow regime of rivers, and it is therefore essential to understand the potential effects of human structures on the hydrological regime of rivers to reduce their destructive impacts. This study analyzes the hydrological changes in the Shahrchai River in response to the Shahrchai Dam construction in Urmia, Iran. The study period was from 1950 to 2017 at the Urmia Band station. The Indicators of Hydrological Alteration (IHA) were used to analyze the hydrological changes before and after regulating, accounting for land use changes and climatic factors. The results revealed the adverse effects of the Shahrchai Dam on the hydrological indices. The analysis showed an increase in the average flow rate during the summer season and a decrease in other seasons. However, the combined effects of water transferring for drinking purposes, a decrease in permanent snow cover upstream of the dam, and an increase in water use for irrigation and agricultural purposes resulted in a decrease in the released river flow. Furthermore, the minimum and maximum daily flow rates decreased by approximately 85% and 65%, respectively, after the construction of the Shahrchai Dam. Additionally, the number of days with maximum flow rates increased from 117 days in the pre-dam period to 181 days in the post-dam period. As a concluding remark, the construction of the Shahrchai Dam, land use/cover changes, and a decrease in permanent snow cover had unfavorable effects on the hydrological regime of the river. Therefore, the hydrological indicators should be adjusted to an acceptable level compared to the natural state to preserve the river ecosystem. The findings of this study are expected to guide water resource managers in regulating the sustainable flow regime of permanent rivers.

Abstract Background The increase in the resistance of bacterial strains to antibiotics has led to research into the bactericidal potential of non-antibiotic compounds. This study aimed to evaluate in vitro antibacterial/ antibiofilm properties of nisin and selenium encapsulated in thiolated chitosan nanoparticles (N/Se@TCsNPs) against prevalent enteric pathogens including standard isolates of Vibrio (V.) cholerae O1 El Tor ATCC 14,035, Campylobacter (C.) jejuni ATCC 29,428, Salmonella (S.) enterica subsp. enterica ATCC 19,430, Shigella (S.) dysenteriae PTCC 1188, Escherichia (E.) coli O157:H7 ATCC 25,922, Listeria (L.) monocytogenes ATCC 19,115, and Staphylococcus (S.) aureus ATCC 29,733. Methods The synthesis and comprehensive analysis of N/Se@TCsNPs have been completed. Antibacterial and antibiofilm capabilities of N/Se@TCsNPs were evaluated through broth microdilution and crystal violet assays. Furthermore, the study included examining the cytotoxic effects on Caco-2 cells and exploring the immunomodulatory effects of N/Se@TCsNPs. This included assessing the levels of both pro-inflammatory (IL-6 and TNFα) and anti-inflammatory (IL-10 and TGFβ) cytokines and determining the gene expression of TLR2 and TLR4. Results The N/Se@TCsNPs showed an average diameter of 136.26 ± 43.17 nm and a zeta potential of 0.27 ± 0.07 mV. FTIR spectroscopy validated the structural features of N/Se@TCsNPs. Scanning electron microscopy (SEM) images confirmed their spherical shape and uniform distribution. Thermogravimetric Analysis (TGA)/Differential Scanning Calorimetry (DSC) tests demonstrated the thermal stability of N/Se@TCsNPs, showing minimal weight loss of 0.03%±0.06 up to 80 °C. The prepared N/Se@TCsNPs showed a thiol content of 512.66 ± 7.33 µmol/g (p

Abstract The purpose of this study was to examine how demographics, etiology, and clinical examination findings are related to visual outcomes in subjects with open globe injury (OGI) across a large and generalizable sample. A retrospective cohort analysis was performed using data collected from the electronic medical records of four tertiary university centers for subjects with OGI presenting from 2018 to 2021. Demographic information, injury mechanisms, clinical exam findings, visual acuity (VA) at presentation and most recent follow-up were recorded. In subjects with bilateral OGIs, only right eyes were included. A modified ocular trauma score (OTS) using presenting VA, the presence of perforating injury, OGI, and afferent pupillary defect was calculated. The risk of subjects’ demographic characteristics, ocular trauma etiology, clinical findings and modified OTS on the presence of monocular blindness at follow-up were assessed using univariable and multivariable regression models. 1426 eyes were identified. The mean age was 48.3 years (SD: ± 22.4 years) and the majority of subjects were men (N = 1069, 75.0%). Univariable analysis demonstrated that subjects of Black race were 66% (OR: 1.66 [1.25–2.20]; P

Abstract Introduction Fear-avoidance beliefs (FAB) play a crucial role in the treatment outcomes of post-surgery patients. These beliefs can lead to activity avoidance, increased pain, and decreased quality of life. Therefore, accurately measuring these beliefs in Iranian patients is of significant importance. The Fear-Avoidance Belief Questionnaire (FABQ) is a patient-reported questionnaire that evaluates individuals’ FAB. Since the validity and reliability of the Persian version of FABQ (FABQ-P) have not been assessed based on the Iranian population and sociocultural contexts, the current study has been implemented to determine the reliability and validity of the FABQ-P among Iranian post-operative patients by translation and psychometric properties. Methods This methodological study conducted in 2023, a sample of 400 patients who had undergone surgery were selected using a convenience sampling method. The scale used in the study was translated and its psychometric properties were evaluated through network analysis and assessments of construct validity (including exploratory and confirmatory factor analysis), convergent validity, and discriminant validity. Additionally, the study assessed the internal consistency of the scale. Results The MLEFA results with Promax and Kaiser Normalization rotation yielded two factors explaining 57.91% of the variance, encompassing 13 items. Also, the model was approved by CFA. Convergent and discriminant validity have been confirmed through the following criteria: Average Variance Extracted (AVE) exceeding 0.5, Composite Reliability (CR) surpassing 0.7, and Heterotrait-Monotrait Ratio of Correlations (HTMT) equating to 0.597. As for reliability, Cronbach’s alpha, composite reliability (CR), and MaxR for all constructs were greater than 0.7, demonstrating good internal consistency. Conclusion As demonstrated by the results, the FABQ-P has a satisfactory level of reliability along with authentic validity according to the sociocultural contexts of Iranian post-operative patients.

Background: The urgent need and craving for smoking are significant aspects of smoking cessation syndrome, often leading to high relapse rates. Therefore, evaluating smokers’ cravings and immediate needs is essential, and the questionnaire on smoking urges (QSU) is a remarkable instrument for this purpose. Objectives: This study aimed to evaluate the reliability and validity of the Persian version of QSU among the general population. Materials & Methods: This is a descriptive cross-sectional study. The studied population includes 200 smokers from the general population referred to Shafa Hospital, Rasht City, Iran, in 2023. The QSU was translated and reviewed by translators and bilingual experts to achieve the final Persian version. We assessed the content validity, reliability, and factor analysis of QSU. The IBM SPSS software, version 26 was used for data analysis. In addition, confirmatory factor analysis was performed using Smart PLS software, version 3.2.9. Results: The results showed that the content validity rate was 0.94. The QSU exhibited a Cronbach α of 0.892, indicating high internal stability of the scale. After presenting the confirmatory factor model, 2 questions from the first factor and 4 questions from the second factor were removed due to their factor loadings of

Background: Assessing the learning environment provides an overview of the current educational status and aids in identifying areas of concern. This study aimed to understand the students’ perceptions of the educational environment at the Paramedical Faculty of Ilam University of Medical Sciences in 2021.Methods: This cross-sectional research was conducted at Ilam University of Medical Sciences, where 300 paramedical students were recruited by census. The Dundee Ready Education Environment Measure (DREEM) was used as the data collection tool. An independent t-test and one-way analysis of variance were used to investigate the relationships between demographic variables and each dimension of the questionnaire. Data analysis was performed using SPSS-26 software.Results: The sample studied included 166 female and 134 male students with an average age of 24.68±7.837 years. The mean and SD of the perception of the educational environment were 112.34 (24.63). There was a significant relationship between marital status, education level, age, and academic semester with students’ understanding of the educational environment (P≤0.05). Twenty percent of the items in the questionnaire indicated a “problematic” situation, while the rest were in a “need of attention” status.Conclusion: The results of this study showed that paramedical students have a positive perception of the educational environment. The lowest mean and SD of the dimensions of the questionnaire were observed in students’ understanding of learning and social self-perception. The other dimensions were in a more satisfactory condition.

Introduction: Cardiovascular disease (CVD) is one of the most important health problems and the leading cause of mortality worldwide. This study aimed to estimate the risk of CVD using the World Health Organization/International Society of Hypertension (WHO/ISH) risk prediction charts. Methods: The demographic characteristics of all participants of this study aged 40-70 years who did not have a prior coronary event were collected. The 10-year CVD risk was estimated using the laboratory version of the WHO/ISH risk score charts. The risk scores for 11678 participants of the Azar cohort population were calculated. Participants were classified as low risk, moderate risk, or high risk. Results: According to the WHO/ISH charts, only 0.1 % of the population was classified as high-risk (≥40%), and 96.8% had a 10-year CVD risk of

Background: In case of inadequacy and inadequacy of parents, issues and problems occur, including child abuse by them or the people around them; Child abuse is one of the big social problems that includes all societies, both advanced and developing, so that the health and comfort of the injured child is endangered. Aims: The purpose of this article is to determine the fit of the prediction model of dark personality traits based on childhood traumas with the mediating role of self-regulation, mentalization and attachment styles. Methods: The current research was of correlational type and according to the objectives, Structural Equation Modeling (SEM) method was also used, based on Klein's formula, 147,400 people were considered. Also, separately from the questionnaires of dark personality traits (Johnson and Webster, 2010), self-regulation by Miller and Brown (1999), adult attachment style of Collins and Reed, (1990), mentalization or reflective performance by Fonagy et al. (2016), trauma Childhood Questionnaire (CTQ) was used by Bernstein, Stein, Newkamp, Walker, Pugh, et al. (2003) to adjust the model. In this research, descriptive statistics of mean, standard deviation, skewness and kurtosis and inferential statistics of correlation, regression and structural equation modeling (SEM) were used to analyze the data. Results: The results of the research show that the direct path of childhood trauma to dark personality traits was not significant, but all the indirect paths were significant, which indicated the confirmation of the mediation of the three variables of self-regulation, mentalization and attachment style (p

Abnormalities of several oncogenes and tumor suppressor genes have been identified in carcinomas during the last decade and of pathological warfare and physiological traits, and multiple genetic changes have been demonstrated in individual carcinomas. We conducted a systematic review of studies enrolling adolescents and adults with Carcinoma, every type in which a cancer intervention was randomized, or all study designs in which there was a primary or secondary outcome. We searched Ovid MEDLINE, EMBASE, and Evidence-Based Medicine Reviews from 1990 to June 2015. Two reviewers evaluated study eligibility and abstracted data. In total, 67 studies were included and consisted of 62 randomized trials, reviews, and 5 studies. None of the studies (0/81) provided a definition. Only one randomized trial provided a definition. We were unable to identify any definitions used in studies of adolescents and adults with Carcinoma. Given that a proportion of this population may receive intensive treatment, there is an urgent need for consensus-based definitions of use across trials and review systematic & and meta-analysis.

Abstract Background Oral lichen planus is a chronic and potentially malignant disorder of oral mucosa. Corticosteroids are used as first-line therapy for oral lichen planus patients; however, they have many side effects. Platelet concentrates (platelet-rich plasma and platelet-rich fibrin) are autologous bioactive materials. This systematic review investigated the effects of autologous platelet concentrates compared to topical steroids in treating symptomatic oral lichen planus patients. Materials and methods A systematic literature search was performed in PubMed, Web of Science, Scopus, Embase, and Cochrane for randomized controlled trials. Preferred Reporting Items for Systematic Reviews and meta-analysis guidelines were observed for article selection. For the pooling of studies, meta-analysis using Standardized Mean Differences by random effects model was carried out to estimate summary effect sizes for the treatment of oral lichen planus. Results A total of six studies, incorporating 109 oral lichen planus patients, were involved. Both treatment modalities showed a statistically significant improvement in the outcome parameters (lesion size, pain score, Thongprasom score) from the baseline to the end of treatment and further to the follow-up visits. There was no significant difference in the pooled estimate SMD of pain decline in patients of the two groups (SMD = 0.17 (95% CI: -0.47 to 0.81); I2 = 63.6%). The SMD of Thongprasom score in patients receiving autologous platelet concentrates was lower than the corticosteroid groups, with no significant effect size (SMD= -2.88 (95% CI: -5.51 to -0.25); I2 = 91.7%). Therefore, there was no statistically significant difference between the autologous platelet concentrates and topical steroids regarding pain and clinical score. Conclusion Autologous platelet concentrates, and topical steroids decreased the size of lesions, Thongprasom scale, and pain in oral lichen planus patients, but the difference between the two treatments was not statistically significant. Thus, autologous platelet concentrates could be considered as an alternative treatment to topical steroids.

Ginsenoside Rg1(Rg1), a monomer of a tetracyclic triterpenoid derivative, possesses diverse medicinal properties attributed to its unique chemical structure and may have beneficial effects on fetal development. This study aimed to investigate the protective effects of prenatal exposure to Rg1 against Methimazole-induced gestational hypothyroidism on reflexive behaviors, conditioned fear, and cortical antioxidant levels in mouse offspring.40 female virgin mice and 12 male NMRI mice were assigned to four groups: group 1 served as the control, group 2 received Methimazole(MMI) at a concentration of 0.02% in their drinking water, group 3 received Rg1(150 mg/kg), and group 4 received both MMI and Rg1.Groups of 2–4 were administered the substances from days 1–9 of gestation. After delivery, pups were selected, and reflexive motor behaviors and conditioned fear were assessed. Additionally, levels of brain tissue catalase(CAT), malondialdehyde(MDA), superoxide dismutase(SOD), and glutathione peroxidase(GPx) levels were measured. Furthermore, postpartum immobility time in the forced swimming test (FST), tail suspension test (TST), and the number of squares crossed in the open field test (OFT)were determined. The results demonstrated that maternal exposure to Rg1 improved ambulation score, hind-limb suspension score, grip strength, front-limb suspension, hind-limb foot angle, negative geotaxis, surface righting, and conditioned fear in hypothyroidism-induced offspring(P

Complex homeostatic control mechanisms are tools to adjust the food birds eat and their appetite. Birds and mammals differ in several ways considering food intake regulation. Therefore, this study aimed to investigate the special effects of the intracerebroventricular (ICV) injection of spexin and its interaction with nitric oxide, serotonin and corticotropin receptors on central food intake regulation in broilers. In the test 1, Broilers received ICV injection of saline, PCPA (p-chlorophenylalanine,1.25 µg), spexin (10 nmol) and PCPA+spexin. In test 2–7, 8-OH-DPAT, SB-242084 (5-HT2C, 1.5 µg), L-arginine (Precursor of nitric oxide, 200 nmol), L-NAME (nitric oxide synthetize inhibitor, 100 nmol), Astressin-B (30 µg) and Astressin2-B (30 µg) were injected to Broilers instead of the PCPA. Then, the amount of food received was measured up to 2 h after the injection. The food consumption was significantly decreased by Spexin (10 nmol) (P