37 results on '"Biotechnology applications"'
Search Results
2. 红酵母基因组和代谢工程改造研究进展.
- Author
-
李雨真, 梅天秀, 李治文, 王淇, 李俊, 邹岳, and 赵心清
- Subjects
- *
MONASCUS purpureus , *GENOME editing , *NUCLEOTIDE sequencing , *ENVIRONMENTAL soil science , *YEAST - Abstract
Red yeast is a general term for a class of red pigment-producing yeast, and they are widely distributed in various ecological environments such as soil, sea, rivers and lakes. Red yeasts have the ability to produce polysaccharides, oils, carotenoids and other active substances and thus own great potential for applications in agriculture, food, medicine and cosmetics. In recent years, studies on red yeast including their applications, genome sequencing and key metabolic enzymes have expanded the in-depth knowledge of this important group of yeast. In addition, progress has been made in genome editing and metabolic engineering modification of these yeasts, providing basis for developing more efficient red yeast strains for industrial applications. Here, we review the applications of red yeast and related research advances in genomic studies and metabolic engineering. This review would be helpful for better understanding the physiology and metabolic features of red yeast, as well as utilization of renewable biomass for sustainable bioproduction using red yeast. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
3. Lipase from Yarrowia lipolytica : Prospects as an Industrial Biocatalyst for Biotechnological Applications.
- Author
-
da Silva, Jessica Lopes, Sales, Misael Bessa, de Castro Bizerra, Viviane, Nobre, Millena Mara Rabelo, de Sousa Braz, Ana Kátia, da Silva Sousa, Patrick, Cavalcante, Antônio L. G., Melo, Rafael L. F., Gonçalves De Sousa Junior, Paulo, Neto, Francisco S., da Fonseca, Aluísio Marques, and Santos, José Cleiton Sousa dos
- Subjects
ENZYMES ,LIPASES ,WASTE treatment ,FOOD industry ,BIOREMEDIATION ,BIOMASS energy - Abstract
This paper aims to present the advances related to the biotechnological application of lipases Y. lipolytica, presenting their properties and more efficient ways to use them in different industrial applications. Waste treatment and bioremediation highlight recent studies and advances and the interest in large-scale applications in the food sector and biofuel production. The USA and China, two major world powers in industy, are of utmost importance in the search for the improvement in the development and properties of a controlled system for the large-scale production of a significant number of applications of lipase from Y. lipolytica. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
4. Microalgae from Cold Environments and Their Possible Biotechnological Applications.
- Author
-
Montuori, Eleonora, Saggiomo, Maria, and Lauritano, Chiara
- Abstract
Cold environments include deep ocean, alpine, and polar areas. Even if the cold conditions are harsh and extreme for certain habitats, various species have been adapted to survive in them. Microalgae are among the most abundant microbial communities which have adapted to live in low light, low temperature, and ice coverage conditions typical of cold environments by activating different stress-responsive strategies. These species have been shown to have bioactivities with possible exploitation capabilities for human applications. Even if they are less explored compared to species living in more accessible sites, various activities have been highlighted, such as antioxidant and anticancer activities. This review is focused on summarizing these bioactivities and discussing the possible exploitation of cold-adapted microalgae. Thanks to the possibility of mass cultivating algae in controlled photobioreactors, eco-sustainable exploitation is in fact possible by sampling a few microalgal cells without impacting the environment. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
5. Epigenetics and Medical Biotechnology
- Author
-
Anwar, Mumtaz, Rashid, Samia, Farooq, Zeenat, Anwar, Mumtaz, editor, Ahmad Rather, Riyaz, editor, and Farooq, Zeenat, editor
- Published
- 2022
- Full Text
- View/download PDF
6. Diagnostic and Therapeutic Biotechnology
- Author
-
Raina, Ashvinder, Villingiri, Vignesh, Jehan, Shafqat, Qadir, Syed Abdul, Anwar, Mumtaz, editor, Ahmad Rather, Riyaz, editor, and Farooq, Zeenat, editor
- Published
- 2022
- Full Text
- View/download PDF
7. La biocalcification bactérienne en milieu marin et ses applications.
- Author
-
Vincent, Julia, Colin, Béatrice, Lanneluc, Isabelle, Refait, Philippe, Sabot, René, Jeannin, Marc, and Sablé, Sophie
- Subjects
BACTERIAL metabolism ,BIOTECHNOLOGY - Abstract
Copyright of Matériaux et Techniques is the property of EDP Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2022
- Full Text
- View/download PDF
8. The impact of teaching via educational films on science teacher candidates' perceptions of biotechnology applications.
- Author
-
Önder, Ayșe Nesibe, Yıldırım, Ezgi Güven, and Önder, İsmail
- Subjects
EDUCATIONAL films ,SCIENCE teachers ,STATE universities & colleges ,BIOTECHNOLOGY ,ACQUISITION of data - Abstract
This study aims to investigate the influence of teaching viaeducational films on science teacher candidates' perceptions of biotechnology applications. This is a mixed-methods study aiming to investigate the influence of teaching with educational films on science teacher candidates' perceptions of biotechnology applications. In the quantitative data collection process of the research, a single group pre-test / post-test experimental design was used. And the phenomenology method was used, on the other hand, to collect the qualitative data of the study. For this research, which aims to investigate the influence of teaching with educational films on science teacher candidates' perceptions of biotechnology applications, 32 second-year science teacher candidates at a state university in Ankara were recruited. Two data collection tools were used in the study. The perception scale for biotechnology applications developed by the researchers was used to collect the quantitative data of the study. While the interview form was used to collect the qualitative data of the study. According to the results the study found that teaching with educational films increased the perceptions of science teacher candidates about biotechnology applications. within the scope of the research, the question of whether teaching a lesson with educational films has an effect on changing their perceptions about the subject, and if so, how this change occurs, was asked during the interview stage, and it was found that the majority of the teacher candidates perceived a meaningful change. [ABSTRACT FROM AUTHOR]
- Published
- 2022
9. Lipase from Yarrowia lipolytica: Prospects as an Industrial Biocatalyst for Biotechnological Applications
- Author
-
Jessica Lopes da Silva, Misael Bessa Sales, Viviane de Castro Bizerra, Millena Mara Rabelo Nobre, Ana Kátia de Sousa Braz, Patrick da Silva Sousa, Antônio L. G. Cavalcante, Rafael L. F. Melo, Paulo Gonçalves De Sousa Junior, Francisco S. Neto, Aluísio Marques da Fonseca, and José Cleiton Sousa dos Santos
- Subjects
Y. lipolytica ,lipase ,industrial ,biotechnology applications ,future ,Fermentation industries. Beverages. Alcohol ,TP500-660 - Abstract
This paper aims to present the advances related to the biotechnological application of lipases Y. lipolytica, presenting their properties and more efficient ways to use them in different industrial applications. Waste treatment and bioremediation highlight recent studies and advances and the interest in large-scale applications in the food sector and biofuel production. The USA and China, two major world powers in industy, are of utmost importance in the search for the improvement in the development and properties of a controlled system for the large-scale production of a significant number of applications of lipase from Y. lipolytica.
- Published
- 2023
- Full Text
- View/download PDF
10. Microalgae from Cold Environments and Their Possible Biotechnological Applications
- Author
-
Eleonora Montuori, Maria Saggiomo, and Chiara Lauritano
- Subjects
polar environments ,microalgae ,stressors ,stress responses ,bioactivity ,biotechnology applications ,Biology (General) ,QH301-705.5 - Abstract
Cold environments include deep ocean, alpine, and polar areas. Even if the cold conditions are harsh and extreme for certain habitats, various species have been adapted to survive in them. Microalgae are among the most abundant microbial communities which have adapted to live in low light, low temperature, and ice coverage conditions typical of cold environments by activating different stress-responsive strategies. These species have been shown to have bioactivities with possible exploitation capabilities for human applications. Even if they are less explored compared to species living in more accessible sites, various activities have been highlighted, such as antioxidant and anticancer activities. This review is focused on summarizing these bioactivities and discussing the possible exploitation of cold-adapted microalgae. Thanks to the possibility of mass cultivating algae in controlled photobioreactors, eco-sustainable exploitation is in fact possible by sampling a few microalgal cells without impacting the environment.
- Published
- 2023
- Full Text
- View/download PDF
11. Biotechnology for Wildlife
- Author
-
Singh, Birbal, Mal, Gorakh, Gautam, Sanjeev K., Mukesh, Manishi, Singh, Birbal, Mal, Gorakh, Gautam, Sanjeev K., and Mukesh, Manishi
- Published
- 2019
- Full Text
- View/download PDF
12. Advances in laccase-triggered anabolism for biotechnology applications.
- Author
-
Sun, Kai, Li, Shunyao, Si, Youbin, and Huang, Qingguo
- Subjects
- *
BIOSYNTHESIS , *POLYMERIZATION , *SUSTAINABLE development , *BIOTECHNOLOGY , *LACCASE , *SUSTAINABLE chemistry , *CATALYSTS , *PLANT pigments - Abstract
This is the first comprehensive overview of laccase-triggered anabolism from fundamental theory to biotechnology applications. Laccase is a typical biological oxidordeuctase that induces the one-electronic transfer of diverse substrates for engendering four phenoxy radicals with concomitant reduction of O2 into 2H2O. In vivo, laccase can participate in anabolic processes to create multifarious functional biopolymers such as fungal pigments, plant lignins, and insect cuticles, using mono/polyphenols and their derivatives as enzymatic substrates, and is thus conducive to biological tissue morphogenesis and global carbon storage. Exhilaratingly, fungal laccase has high redox potential (E° = 500–800 mV) and thermodynamic efficiency, making it a remarkable candidate for utilization as a versatile catalyst in the green and circular economy. This review elaborates the anabolic mechanisms of laccase in initiating the polymerization of natural phenolic compounds and their derivatives in vivo via radical-based self/cross-coupling. Information is also presented on laccase immobilization engineering that expands the practical application ranges of laccase in biotechnology by improving the enzymatic catalytic activity, stability, and reuse rate. Particularly, advances in biotechnology applications in vitro through fungal laccase-triggered macromolecular biosynthesis may provide a key research direction beneficial to the rational design of green chemistry. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
13. Synthesis of selected unnatural sugar nucleotides for biotechnological applications.
- Author
-
Qiao, Meng, Li, Bingzhi, Ji, Yuan, Lin, Lei, Linhardt, Robert, and Zhang, Xing
- Subjects
- *
SUGAR , *NUCLEOTIDES , *MONOSACCHARIDES , *CARBOHYDRATE metabolism , *URIDINE diphosphate , *CARBOHYDRATES - Abstract
Sugar nucleotides are the principal building blocks for the synthesis of most complex carbohydrates and are crucial intermediates in carbohydrate metabolism. Uridine diphosphate (UDP) monosaccharides are among the most common sugar nucleotide donors and are transferred to glycosyl acceptors by glycosyltransferases or synthases in glycan biosynthetic pathways. These natural nucleotide donors have great biological importance, however, the synthesis and application of unnatural sugar nucleotides that are not available from in vivo biosynthesis are not well explored. In this review, we summarize the progress in the preparation of unnatural sugar nucleotides, in particular, the widely studied UDP-GlcNAc/GalNAc analogs. We focus on the "two-block" synthetic pathway that is initiated from monosaccharides, in which the first block is the synthesis of sugar-1-phosphate and the second block is the diphosphate bond formation. The biotechnological applications of these unnatural sugar nucleotides showing their physiological and pharmacological potential are discussed. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
14. Expanding the Knowledge on the Skillful Yeast Cyberlindnera jadinii.
- Author
-
Sousa-Silva, Maria, Vieira, Daniel, Soares, Pedro, Casal, Margarida, and Soares-Silva, Isabel
- Subjects
- *
SINGLE cell proteins , *PHARMACEUTICAL industry , *ORGANIC acids , *PHYLOGENY , *MEMBRANE transport proteins - Abstract
Cyberlindnera jadinii is widely used as a source of single-cell protein and is known for its ability to synthesize a great variety of valuable compounds for the food and pharmaceutical industries. Its capacity to produce compounds such as food additives, supplements, and organic acids, among other fine chemicals, has turned it into an attractive microorganism in the biotechnology field. In this review, we performed a robust phylogenetic analysis using the core proteome of C. jadinii and other fungal species, from Asco- to Basidiomycota, to elucidate the evolutionary roots of this species. In addition, we report the evolution of this species nomenclature over-time and the existence of a teleomorph (C. jadinii) and anamorph state (Candida utilis) and summarize the current nomenclature of most common strains. Finally, we highlight relevant traits of its physiology, the solute membrane transporters so far characterized, as well as the molecular tools currently available for its genomic manipulation. The emerging applications of this yeast reinforce its potential in the white biotechnology sector. Nonetheless, it is necessary to expand the knowledge on its metabolism, regulatory networks, and transport mechanisms, as well as to develop more robust genetic manipulation systems and synthetic biology tools to promote the full exploitation of C. jadinii. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
15. Biotechnology Applications of Cell-Free Expression Systems
- Author
-
August Brookwell, Javin P. Oza, and Filippo Caschera
- Subjects
cell-free expression systems ,cell-free protein synthesis ,biotechnology applications ,synthetic biology ,metabolic engineering ,prototyping ,Science - Abstract
Cell-free systems are a rapidly expanding platform technology with an important role in the engineering of biological systems. The key advantages that drive their broad adoption are increased efficiency, versatility, and low cost compared to in vivo systems. Traditionally, in vivo platforms have been used to synthesize novel and industrially relevant proteins and serve as a testbed for prototyping numerous biotechnologies such as genetic circuits and biosensors. Although in vivo platforms currently have many applications within biotechnology, they are hindered by time-constraining growth cycles, homeostatic considerations, and limited adaptability in production. Conversely, cell-free platforms are not hindered by constraints for supporting life and are therefore highly adaptable to a broad range of production and testing schemes. The advantages of cell-free platforms are being leveraged more commonly by the biotechnology community, and cell-free applications are expected to grow exponentially in the next decade. In this study, new and emerging applications of cell-free platforms, with a specific focus on cell-free protein synthesis (CFPS), will be examined. The current and near-future role of CFPS within metabolic engineering, prototyping, and biomanufacturing will be investigated as well as how the integration of machine learning is beneficial to these applications.
- Published
- 2021
- Full Text
- View/download PDF
16. Epigenetic manipulation of filamentous fungi for biotechnological applications: a systematic review.
- Author
-
Poças-Fonseca, Marcio José, Cabral, Camila Gomes, and Manfrão-Netto, João Heitor Colombelli
- Subjects
EPIGENOMICS ,FILAMENTOUS fungi ,EPIGENETICS ,CANDIDA albicans ,DELETION mutation ,FUNGI ,POST-translational modification - Abstract
The study of the epigenetic regulation of gene function has reached pivotal importance in life sciences in the last decades. The mechanisms and effects of processes such as DNA methylation, histone posttranslational modifications and non-coding RNAs, as well as their impact on chromatin structure and dynamics, are clearly involved in physiology homeostasis in plants, animals and microorganisms. In the fungal kingdom, studies on the model yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe contributed enormously to the elucidation of the eukaryote epigenetic landscape. Epigenetic regulation plays a central role in the expression of virulence attributes of human pathogens such as Candida albicans. In this article, we review the most recent studies on the effects of drugs capable of altering epigenetic states and on the impact of chromatin structure-related genes deletion in filamentous fungi. Emphasis is given on plant and insect pathogens, endophytes, secondary metabolites and cellulases/xylanases producing species. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
17. Editorial: Mining, Designing, Mechanisms and Applications of Extremophilic Enzymes.
- Author
-
Fenice, Massimiliano, Khare, Sunil Kumar, and Gorrasi, Susanna
- Subjects
ENZYMES ,HIGH throughput screening (Drug development) ,LIPASES ,XYLANS - Abstract
Another winning strategy, which is expected to improve enzyme properties for biotechnological application, is protein engineering (Xiao et al., [6]; Liu et al., [2]). Keywords: extremophiles; extremozymes; enzyme mining; enzyme purification; enzyme characterization; enzyme structure; biotechnology applications; protein engineering EN extremophiles extremozymes enzyme mining enzyme purification enzyme characterization enzyme structure biotechnology applications protein engineering 1 3 3 10/29/21 20211025 NES 211025 Enzymes play very important roles in industrial and environmental biotechnology. Since DNA manipulations and protein-engineering are quite unsatisfactory in generating extremophilic xylanolytic enzymes, metagenomic approaches have been successfully employed to uncover hidden genes that were inaccessible by culture-dependent approaches. Extremophiles, extremozymes, enzyme purification, enzyme characterization, enzyme structure, biotechnology applications, enzyme mining, protein engineering. [Extracted from the article]
- Published
- 2021
- Full Text
- View/download PDF
18. Expanding the Knowledge on the Skillful Yeast Cyberlindnera jadinii
- Author
-
Maria Sousa-Silva, Daniel Vieira, Pedro Soares, Margarida Casal, and Isabel Soares-Silva
- Subjects
Cyberlindnera jadinii ,phylogeny ,life cycle ,genome ,physiology ,biotechnology applications ,Biology (General) ,QH301-705.5 - Abstract
Cyberlindnera jadinii is widely used as a source of single-cell protein and is known for its ability to synthesize a great variety of valuable compounds for the food and pharmaceutical industries. Its capacity to produce compounds such as food additives, supplements, and organic acids, among other fine chemicals, has turned it into an attractive microorganism in the biotechnology field. In this review, we performed a robust phylogenetic analysis using the core proteome of C. jadinii and other fungal species, from Asco- to Basidiomycota, to elucidate the evolutionary roots of this species. In addition, we report the evolution of this species nomenclature over-time and the existence of a teleomorph (C. jadinii) and anamorph state (Candida utilis) and summarize the current nomenclature of most common strains. Finally, we highlight relevant traits of its physiology, the solute membrane transporters so far characterized, as well as the molecular tools currently available for its genomic manipulation. The emerging applications of this yeast reinforce its potential in the white biotechnology sector. Nonetheless, it is necessary to expand the knowledge on its metabolism, regulatory networks, and transport mechanisms, as well as to develop more robust genetic manipulation systems and synthetic biology tools to promote the full exploitation of C. jadinii.
- Published
- 2021
- Full Text
- View/download PDF
19. Potential Applications of the Escherichia coli Heat Shock Response in Synthetic Biology.
- Author
-
Rodrigues, Joana L. and Rodrigues, Lígia R.
- Subjects
- *
ESCHERICHIA coli , *HEAT shock proteins , *SYNTHETIC biology , *RNA , *BIOSENSORS , *MICROBIAL cells - Abstract
The Escherichia coli heat shock response (HSR) is a complex mechanism triggered by heat shock and by a variety of other growth-impairing stresses. We explore here the potential use of the E. coli HSR mechanism in synthetic biology approaches. Several components of the regulatory mechanism (such as heat shock promoters, proteins, and RNA thermosensors) can be extremely valuable in the creation of a toolbox of well-characterized biological parts to construct biosensors or microbial cell factories with applications in the environment, industry, or healthcare. In the future, these systems can be used for instance to detect a pollutant in water, to regulate and optimize the production of a compound with industrial relevance, or to administer a therapeutic agent in vivo . [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
20. Applying a text mining framework to the extraction of numerical parameters from scientific literature in the biotechnology domain
- Author
-
Anália LOURENÇO, Regina NOGUEIRA, and André SANTOS
- Subjects
Text mining ,Biotechnology applications ,Procedure optimization ,Electronic computers. Computer science ,QA75.5-76.95 - Abstract
Scientific publications are the main vehicle to disseminate information in the field of biotechnology for wastewater treatment. Indeed, the new research paradigms and the application of high-throughput technologies have increased the rate of publication considerably. The problem is that manual curation becomes harder, prone-to-errors and time-consuming, leading to a probable loss of information and inefficient knowledge acquisition. As a result, research outputs are hardly reaching engineers, hampering the calibration of mathematical models used to optimize the stability and performance of biotechnological systems. In this context, we have developed a data curation workflow, based on text mining techniques, to extract numerical parameters from scientific literature, and applied it to the biotechnology domain. A workflow was built to process wastewater-related articles with the main goal of identifying physico-chemical parameters mentioned in the text. This work describes the implementation of the workflow, identifies achievements and current limitations in the overall process, and presents the results obtained for a corpus of 50 full-text documents.
- Published
- 2013
21. Applying a text mining framework to the extraction of numerical parameters from scientific literature in the biotechnology domain
- Author
-
André SANTOS, Regina NOGUEIRA, and Anália LOURENÇO
- Subjects
text mining ,biotechnology applications ,procedure optimization ,Electronic computers. Computer science ,QA75.5-76.95 - Abstract
Scientific publications are the main vehicle to disseminate information in the field of biotechnology for wastewater treatment. Indeed, the new research paradigms and the application of high-throughput technologies have increased the rate of publication considerably. The problem is that manual curation becomes harder, prone-to-errors and time-consuming, leading to a probable loss of information and inefficient knowledge acquisition. As a result, research outputs are hardly reaching engineers, hampering the calibration of mathematical models used to optimize the stability and performance of biotechnological systems. In this context, we have developed a data curation workflow, based on text mining techniques, to extract numerical parameters from scientific literature, and applied it to the biotechnology domain. A workflow was built to process wastewater-related articles with the main goal of identifying physico-chemical parameters mentioned in the text. This work describes the implementation of the workflow, identifies achievements and current limitations in the overall process, and presents the results obtained for a corpus of 50 full-text documents.
- Published
- 2012
- Full Text
- View/download PDF
22. Biotechnology Applications of Cell-Free Expression Systems
- Author
-
Filippo Caschera, August Brookwell, and Javin Oza
- Subjects
biomanufacturing ,Science ,prototyping ,Paleontology ,cell-free expression systems ,Review ,General Biochemistry, Genetics and Molecular Biology ,cell-free protein synthesis ,machine learning ,Space and Planetary Science ,biotechnology applications ,synthetic biology ,metabolic engineering ,Ecology, Evolution, Behavior and Systematics - Abstract
Cell-free systems are a rapidly expanding platform technology with an important role in the engineering of biological systems. The key advantages that drive their broad adoption are increased efficiency, versatility, and low cost compared to in vivo systems. Traditionally, in vivo platforms have been used to synthesize novel and industrially relevant proteins and serve as a testbed for prototyping numerous biotechnologies such as genetic circuits and biosensors. Although in vivo platforms currently have many applications within biotechnology, they are hindered by time-constraining growth cycles, homeostatic considerations, and limited adaptability in production. Conversely, cell-free platforms are not hindered by constraints for supporting life and are therefore highly adaptable to a broad range of production and testing schemes. The advantages of cell-free platforms are being leveraged more commonly by the biotechnology community, and cell-free applications are expected to grow exponentially in the next decade. In this study, new and emerging applications of cell-free platforms, with a specific focus on cell-free protein synthesis (CFPS), will be examined. The current and near-future role of CFPS within metabolic engineering, prototyping, and biomanufacturing will be investigated as well as how the integration of machine learning is beneficial to these applications.
- Published
- 2021
23. Editorial: Mining, Designing, Mechanisms and Applications of Extremophilic Enzymes
- Author
-
Massimiliano, Fenice, Sunil Kumar, Khare, and Susanna, Gorrasi
- Subjects
Editorial ,enzyme mining ,biotechnology applications ,protein engineering ,enzyme purification ,extremozymes ,Microbiology ,extremophiles ,enzyme characterization ,enzyme structure - Published
- 2021
24. Expanding the knowledge on the skillful yeast Cyberlindnera jadinii
- Author
-
Daniel Vieira, Pedro Soares, Margarida Casal, Isabel Soares-Silva, Maria Sousa-Silva, and Universidade do Minho
- Subjects
Microbiology (medical) ,Physiology ,Life cycle ,Plant Science ,Computational biology ,Biology ,Genome ,Membrane transporter systems ,Cyberlindnera jadinii ,03 medical and health sciences ,Synthetic biology ,Phylogenetics ,lcsh:QH301-705.5 ,Ecology, Evolution, Behavior and Systematics ,Phylogeny ,030304 developmental biology ,2. Zero hunger ,0303 health sciences ,Torulopsis utilis ,Science & Technology ,Phylogenetic tree ,030306 microbiology ,Membrane Transporters ,Yeast ,lcsh:Biology (General) ,Proteome ,Biotechnology applications - Abstract
Cyberlindnera jadinii is widely used as a source of single-cell protein and is known for its ability to synthesize a great variety of valuable compounds for the food and pharmaceutical industries. Its capacity to produce compounds such as food additives, supplements, and organic acids, among other fine chemicals, has turned it into an attractive microorganism in the biotechnology field. In this review, we performed a robust phylogenetic analysis using the core proteome of C. jadinii and other fungal species, from Asco- to Basidiomycota, to elucidate the evolutionary roots of this species. In addition, we report the evolution of this species nomenclature over-time and the existence of a teleomorph (C. jadinii) and anamorph state (Candida utilis) and summarize the current nomenclature of most common strains. Finally, we highlight relevant traits of its physiology, the solute membrane transporters so far characterized, as well as the molecular tools currently available for its genomic manipulation. The emerging applications of this yeast reinforce its potential in the white biotechnology sector. Nonetheless, it is necessary to expand the knowledge on its metabolism, regulatory networks, and transport mechanisms, as well as to develop more robust genetic manipulation systems and synthetic biology tools to promote the full exploitation of C. jadinii., This work was supported by the strategic program UID/BIA/04050/2019, funded by Portuguese funds through the FCT I.P., the projects: PTDC/BIAMIC/5184/2014, funded by national funds through the Fundação para a Ciência e Tecnologia (FCT) I.P. and by the European Regional Development Fund (ERDF) through the COMPETE 2020–Programa Operacional Competitividade e Internacionalização (POCI), and EcoAgriFood: Innovative green products and processes to promote AgriFood BioEconomy (operação NORTE-01–0145-FEDER-000009), supported by Norte Portugal Regional Operational Program (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF). M.S.S. acknowledges the Norte2020 for the UMINHO/BD/25/2016 PhD grant with the reference NORTE-08–5369-FSE-000060.
- Published
- 2021
25. Expanding the Knowledge on the Skillful Yeast
- Author
-
Maria, Sousa-Silva, Daniel, Vieira, Pedro, Soares, Margarida, Casal, and Isabel, Soares-Silva
- Subjects
biotechnology applications ,physiology ,life cycle ,Review ,Cyberlindnera jadinii ,membrane transporter systems ,phylogeny ,genome - Abstract
Cyberlindnera jadinii is widely used as a source of single-cell protein and is known for its ability to synthesize a great variety of valuable compounds for the food and pharmaceutical industries. Its capacity to produce compounds such as food additives, supplements, and organic acids, among other fine chemicals, has turned it into an attractive microorganism in the biotechnology field. In this review, we performed a robust phylogenetic analysis using the core proteome of C. jadinii and other fungal species, from Asco- to Basidiomycota, to elucidate the evolutionary roots of this species. In addition, we report the evolution of this species nomenclature over-time and the existence of a teleomorph (C. jadinii) and anamorph state (Candida utilis) and summarize the current nomenclature of most common strains. Finally, we highlight relevant traits of its physiology, the solute membrane transporters so far characterized, as well as the molecular tools currently available for its genomic manipulation. The emerging applications of this yeast reinforce its potential in the white biotechnology sector. Nonetheless, it is necessary to expand the knowledge on its metabolism, regulatory networks, and transport mechanisms, as well as to develop more robust genetic manipulation systems and synthetic biology tools to promote the full exploitation of C. jadinii.
- Published
- 2020
26. Actividad antimicrobiana de Weissella confusa y sus metabolitos frente a Escherichia coli y Klebsiella pneumoniae.
- Author
-
Serna Cock, Liliana and Elena Enríquez Valencia, Cruz
- Subjects
ANTI-infective agents ,METABOLITES ,ESCHERICHIA coli ,KLEBSIELLA pneumoniae ,KLEBSIELLA ,BIOTECHNOLOGY - Abstract
Copyright of Revista Colombiana de Biotecnología is the property of Universidad Nacional de Colombia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2013
- Full Text
- View/download PDF
27. Advances in genome studies: the PAG 2010 conference.
- Author
-
Appels, R., Barrerro, R., Keeble, G., and Bellgard, M.
- Subjects
- *
PLANT gene mapping , *ANIMAL genome mapping , *BIOTECHNOLOGY , *HUMAN genome , *GENOMICS - Abstract
An overview is provided of the advances in plant, animal and human genome studies by summarizing the contents of seven plenary lectures presented at the Plant and Animal Genome (PAG) meeting in January 2010. The area of biology covered was wide and reflected the nature of this fast moving science. [ABSTRACT FROM AUTHOR]
- Published
- 2010
- Full Text
- View/download PDF
28. Biotechnology Applications of Cell-Free Expression Systems.
- Author
-
Brookwell, August, Oza, Javin P., and Caschera, Filippo
- Subjects
- *
BIOENGINEERING , *BIOTECHNOLOGY , *ENGINEERING systems , *BIOLOGICAL systems , *PROTEIN synthesis , *SYNTHETIC biology - Abstract
Cell-free systems are a rapidly expanding platform technology with an important role in the engineering of biological systems. The key advantages that drive their broad adoption are increased efficiency, versatility, and low cost compared to in vivo systems. Traditionally, in vivo platforms have been used to synthesize novel and industrially relevant proteins and serve as a testbed for prototyping numerous biotechnologies such as genetic circuits and biosensors. Although in vivo platforms currently have many applications within biotechnology, they are hindered by time-constraining growth cycles, homeostatic considerations, and limited adaptability in production. Conversely, cell-free platforms are not hindered by constraints for supporting life and are therefore highly adaptable to a broad range of production and testing schemes. The advantages of cell-free platforms are being leveraged more commonly by the biotechnology community, and cell-free applications are expected to grow exponentially in the next decade. In this study, new and emerging applications of cell-free platforms, with a specific focus on cell-free protein synthesis (CFPS), will be examined. The current and near-future role of CFPS within metabolic engineering, prototyping, and biomanufacturing will be investigated as well as how the integration of machine learning is beneficial to these applications. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
29. Potential Applications of the Escherichia coli Heat Shock Response in Synthetic Biology
- Author
-
Joana Lúcia Lima Correia Rodrigues, Lígia R. Rodrigues, and Universidade do Minho
- Subjects
0301 basic medicine ,Hot Temperature ,030106 microbiology ,Bioengineering ,Biosensing Techniques ,Biology ,medicine.disease_cause ,03 medical and health sciences ,Synthetic biology ,Bacterial Proteins ,Genes, Reporter ,medicine ,Escherichia coli ,Heat shock ,Promoter Regions, Genetic ,Heat shock response ,Science & Technology ,business.industry ,Mechanism (biology) ,E. coli ,Electrochemical Techniques ,Gene Expression Regulation, Bacterial ,Thermosensors ,3. Good health ,Biotechnology ,Shock (mechanics) ,030104 developmental biology ,Biosensors ,Synthetic Biology ,Biochemical engineering ,Biotechnology applications ,business ,Heat-Shock Response - Abstract
The Escherichia coli heat shock response (HSR) is a complex mechanism triggered by heat shock and by a variety of other growth-impairing stresses. We explore here the potential use of the E. coli HSR mechanism in synthetic biology approaches. Several components of the regulatory mechanism (such as heat shock promoters, proteins, and RNA thermosensors) can be extremely valuable in the creation of a toolbox of well-characterized biological parts to construct biosensors or microbial cell factories with applications in the environment, industry, or healthcare. In the future, these systems can be used for instance to detect a pollutant in water, to regulate and optimize the production of a compound with industrial relevance, or to administer a therapeutic agent in vivo., This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2013 unit and COMPETE 2020 (POCI-01-0145-FEDER-006684) and under the scope of the Project RECI/BBB-EBI/0179/2012 (FCOMP-01-0124-FEDER-027462). The authors also acknowledge financial support from BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020–Programa Operacional Regional do Norte, and a Postdoctoral grant (UMINHO/BPD/37/2015) to J.L.R. funded by the FCT., info:eu-repo/semantics/publishedVersion
- Published
- 2017
30. Applying a text mining framework to the extraction of numerical parameters from scientific literature in the biotechnology domain
- Author
-
Regina Nogueira, Anália Lourenço, André L. Santos, and Universidade do Minho
- Subjects
Text mining ,Computer science ,Process (engineering) ,Context (language use) ,02 engineering and technology ,Scientific literature ,Field (computer science) ,lcsh:QA75.5-76.95 ,030218 nuclear medicine & medical imaging ,Domain (software engineering) ,03 medical and health sciences ,0302 clinical medicine ,0202 electrical engineering, electronic engineering, information engineering ,General Environmental Science ,Science & Technology ,Data curation ,business.industry ,General Engineering ,Procedure optimization ,Data science ,Knowledge acquisition ,6. Clean water ,Biotechnology ,Workflow ,General Earth and Planetary Sciences ,020201 artificial intelligence & image processing ,Biotechnology applications ,lcsh:Electronic computers. Computer science ,business - Abstract
Scientific publications are the main vehicle to disseminate information in the field of biotechnology for wastewater treatment. Indeed, the new research paradigms and the application of high-throughput technologies have increased the rate of publication considerably. The problem is that manual curation becomes harder, prone-to-errors and time-consuming, leading to a probable loss of information and inefficient knowledge acquisition. As a result, research outputs are hardly reaching engineers, hampering the calibration of mathematical models used to optimize the stability and performance of biotechnological systems. In this context, we have developed a data curation workflow, based on text mining techniques, to extract numerical parameters from scientific literature, and applied it to the biotechnology domain. A workflow was built to process wastewater-related articles with the main goal of identifying physico-chemical parameters mentioned in the text. This work describes the implementation of the workflow, identifies achievements and current limitations in the overall process, and presents the results obtained for a corpus of 50 full-text documents.
- Published
- 2013
31. Distinct position-specific sequence features of hexa-peptides that form amyloid-fibrils: Application to discriminate between amyloid fibril and amorphous ?-aggregate forming peptide sequences
- Author
-
A. Mary Thangakani, M. Michael Gromiha, Sandeep Kumar, and Devadasan Velmurugan
- Subjects
Aggregates ,Amyloid ,Peptide ,Sequence (biology) ,Biology ,Statistical potential ,Fibril ,chemistry ,Biochemistry ,Cutoff frequency ,Potential energy ,Structural Biology ,Artificial Intelligence ,mental disorders ,energy metabolism ,Humans ,Amino acid compositions ,human ,Amino Acid Sequence ,Peptide sequence ,Molecular Biology ,Well-defined morphologies ,Glycoproteins ,chemistry.chemical_classification ,10-fold cross-validation ,Statistical discriminators ,Applied Mathematics ,HEXA ,peptide ,Amino acid ,Computer Science Applications ,Proceedings ,Sensitivity and specificity ,Total potential energy ,chemical phenomena ,Amino acids ,Biotechnology applications ,Peptides ,metabolism ,Hydrophobic and Hydrophilic Interactions - Abstract
Background Comparison of short peptides which form amyloid-fibrils with their homologues that may form amorphous β-aggregates but not fibrils, can aid development of novel amyloid-containing nanomaterials with well defined morphologies and characteristics. The knowledge gained from the comparative analysis could also be applied towards identifying potential aggregation prone regions in proteins, which are important for biotechnology applications or have been implicated in neurodegenerative diseases. In this work we have systematically analyzed a set of 139 amyloid-fibril hexa-peptides along with a highly homologous set of 168 hexa-peptides that do not form amyloid fibrils for their position-wise as well as overall amino acid compositions and averages of 49 selected amino acid properties. Results Amyloid-fibril forming peptides show distinct preferences and avoidances for amino acid residues to occur at each of the six positions. As expected, the amyloid fibril peptides are also more hydrophobic than non-amyloid peptides. We have used the results of this analysis to develop statistical potential energy values for the 20 amino acid residues to occur at each of the six different positions in the hexa-peptides. The distribution of the potential energy values in 139 amyloid and 168 non-amyloid fibrils are distinct and the amyloid-fibril peptides tend to be more stable (lower total potential energy values) than non-amyloid peptides. The average frequency of occurrence of these peptides with lower than specific cutoff energies at different positions is 72% and 50%, respectively. The potential energy values were used to devise a statistical discriminator to distinguish between amyloid-fibril and non-amyloid peptides. Our method could identify the amyloid-fibril forming hexa-peptides to an accuracy of 89%. On the other hand, the accuracy of identifying non-amyloid peptides was only 54%. Further attempts were made to improve the prediction accuracy via machine learning. This resulted in an overall accuracy of 82.7% with the sensitivity and specificity of 81.3% and 83.9%, respectively, in 10-fold cross-validation method. Conclusions Amyloid-fibril forming hexa-peptides show position specific sequence features that are different from those which may form amorphous β-aggregates. These positional preferences are found to be important features for discriminating amyloid-fibril forming peptides from their homologues that don't form amyloid-fibrils.
- Published
- 2013
32. White rot fungi laccases for biotechnological applications
- Author
-
Pedro Darío Zapata, María Isabel Fonseca, Martin Giorgio, and Laura Lidia Villalba
- Subjects
BIOTECHNOLOGY APPLICATIONS ,LIGNINOLYTIC ENZYMES ,Environmental pollution ,Biology ,Cell wall ,Ciencias Biológicas ,Patents as Topic ,Industrial Microbiology ,Botany ,Molecular Biology ,LACCASES ,Genetics (clinical) ,Laccase ,chemistry.chemical_classification ,Fungi ,General Medicine ,Industrial microbiology ,Enzyme ,chemistry ,Biochemistry ,WHITE ROT FUNGI ,White rot ,Substrate specificity ,Molecular oxygen ,Micología ,CIENCIAS NATURALES Y EXACTAS ,Biotechnology - Abstract
White rot fungi have an enzymatic system producing oxidative and hydrolytic enzymes that act on the degradation of certain components of the cell wall. They can be applied in several technological processes, such as paper industry, bio-fuels and environmental pollution. Laccases are multi-copper enzymes of wide substrate specificity and high non-specific oxidation capacity that use molecular oxygen to oxidize various aromatic compounds, and are highly relevant biotechnological applications. In this review, we present some significant patents on laccase production and recombinant DNA technology for diverse biotechnology applications. Fil: Villalba, Laura L.. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina Fil: Fonseca, Maria Isabel. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina Fil: Giorgio, Ernesto Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina Fil: Zapata, Pedro Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
- Published
- 2010
33. Engineering stem cell fate with biochemical and biomechanical properties of microcarriers
- Author
-
UCL - SST/ELI/ELIM - Applied Microbiology, UCL - SST/ISV - Institut des sciences de la vie, Florida State University - Department of Chemical and Biomedical Engineering, Sart, Sébastien, Agathos, Spiros N., Li, Yan, UCL - SST/ELI/ELIM - Applied Microbiology, UCL - SST/ISV - Institut des sciences de la vie, Florida State University - Department of Chemical and Biomedical Engineering, Sart, Sébastien, Agathos, Spiros N., and Li, Yan
- Abstract
Microcarriers have been widely used for various biotechnology applications because of their high scale-up potential, high reproducibility in regulating cellular behavior, and well-documented compliance with current Good Manufacturing Practices (cGMP). Recently, microcarriers have been emerging as a novel approach for stem cell expansion and differentiation, enabling potential scale-up of stem cell-derived products in large bioreactors. This review summarizes recent advances of using microcarriers in mesenchymal stem cell (MSC) and pluripotent stem cell (PSC) cultures. From the reported data, efficient expansion and differentiation of stem cells on microcarriers rely on their ability to modulate cell shape (i.e. round or spreading) and cell organization (i.e. aggregate size). Nonetheless, current screening of microcarriers remains empirical, and accurate understanding of how stem cells interact with microcarriers still remains unknown. This review suggests that accurate characterization of biochemical and biomechanical properties of microcarriers is required to fully exploit their potential in regulating stem cell fate decision. Due to the variety of microcarriers, such detailed analyses should lead to the rational design of application-specific microcarriers, enabling the exploitation of reproducible effects for large scale biomedical applications
- Published
- 2013
34. Χαμηλού κόστους χαμηλής κατανάλωσης ενσωματωμένοι επεξεργαστές για βιοτεχνολογικές on-line monitoring εφαρμογές
- Author
-
Γκούτης, Κωνσταντίνος, Kritikakou, Angeliki, Στουραίτης, Αθανάσιος, and Αλεξίου, Γεώργιος
- Subjects
Low energy ,Embedded processors ,Low cost ,Datapath ,ASIP ,Ενσωματωμένοι επεξεργαστές ,Biotechnology applications ,004.6 ,Χαμηλή κατανάλωση ενέργειας ,Βιοτεχνολογικές εφαρμογές ,Χαμηλό κόστος - Abstract
On-line monitoring is an important challenge in future biotechnology applications, for instance in the domain of precision livestock farming, there is need for low-cost intelligent sensors to monitor animal welfare. The common way of observing a living organism is usually done by audio-visual ways performed by a human being, who is present on the scene. This method is, however, subjective, expensive, error prone and time consuming. Instead of performing an animal observation by a human being, automated objective surveillance, by means of low cost intelligent image sensors, can be used. With the use of cheap image sensors and with the help of image analysis techniques, an automated, objective, contact-less monitoring method of the behavior of the living organisms can be provided. Much knowledge has been obtained in the development and use of image analysis algorithms to automatically quantify body features of animals, their activity rate and their behavior. Such an automatic image analysis algorithm is combined with on-line modeling techniques in order to develop an application for the recognition of several behavioral phenotypes of laying hens. The procedure is divided in two phases, where an automatic computer vision algorithm detects the monitoring object from images captured by a video camera, and then another algorithm tracks the detected object through successive frames. Further work is required to integrate these algorithms into low-cost low-energy processing platforms, including embedded systems or even wearable devices. Only then, this important biotechnology development will lead to economically applicable solutions. The challenge of the present thesis especially includes the exploration of ultra-low energy implementation platforms of this biotechnology application. The initial application is developed in the MATLAB environment and is converted to C programming language. Dynamic range and precision analysis are performed to efficiently determine the required fixed-point word-lengths of the application’s variables. Finally, platform-independent and platform-dependent code transformations and integration of the algorithm to different ASIPs (Application Specific Instruction Processors) architectures are applied in order to achieve ultimate low energy consumption. Η On-line παρακολούθηση αποτελεί μια σημαντική πρόκληση στις μελλοντικές βιοτεχνολογικές εφαρμογές, όπως παραδείγματος χάριν στον τομέα της κτηνοτροφίας, όπου είναι επιτακτική η ανάγκη χρήσης χαμηλού κόστους έξυπνων αισθητήρων στην παρακολούθηση της ευημερίας των ζωντανών οργανισμών. Ο συνήθης τρόπος παρακο-λούθησης ενός ζωντανού οργανισμού συνίσταται στη χρήση οπτικοακουστικών μέσων, τα οποία χειρίζεται ο ανθρώπινος παράγοντας που είναι παρόν. Η μέθοδος αυτή είναι κατά κύριο λόγο υποκειμενική, ακριβή, επιρρεπής σε σφάλματα και επιπροσθέτως χρονοβόρα. Αντ’ αυτού είναι δυνατό να εφαρμοσθεί μια αυτοματοποιημένη αντι-κειμενική επιτήρηση, η οποία λαμβάνει χώρα μέσω χαμηλού κόστους έξυπνων αισθητήρων εικόνας. Η χρήση των αισθητήρων, σε συνδυασμό με τη βοήθεια τεχνικών ανάλυσης εικόνας, παράγει μια αυτόματη αντικειμενική και εξ’ αποστάσεως μέθοδο πα-ρακολούθησης της συμπεριφοράς των ζωντανών οργανισμών. Τα τελευταία χρόνια η τεχνογνωσία στην ανάπτυξη και τη χρήση αλγορίθμων επεξεργασίας εικόνας, οι οποίοι εντοπίζουν αυτόματα τα χαρακτηριστικά των σωμάτων των ζωντανών οργανισμών, το ποσοστό δραστηριότητάς τους, καθώς και την συμπε-ριφορά τους, αναπτύσσεται ραγδαία. Ένας τέτοιος αλγόριθμος συνδυάζεται με on-line τεχνικές μοντελοποίησης αποσκοπώντας στην αναγνώριση διαφόρων φαινοτύπων συ-μπεριφοράς των ορνίθων. Η διαδικασία χωρίζεται σε δύο φάσεις, όπου κατά την πρώτη ένας αλγόριθμος με υπολογιστική όραση ανιχνεύει το αντικέιμενο παρακολούθησης από εικόνες που προέρχονται από μια κάμερα παρακολούθησης, και κατά τη δεύτερη ένας αλγόριθμος εντοπισμού αναλαμβάνει την παρακολούθηση του αντικειμένου σε δια-δοχικές εικόνες. Ακολούθως, είναι επιτακτική η υλοποίηση των αλγορίθμων σε χαμηλού κόστους χαμηλής κατανάλωσης πλατφόρμες επεξεργασίας, οι οποίες μπορούν να περιλαμβάνουν ενσωματωμένα ή ακόμα και ασύρματα συστήματα, ούτως ώστε η σημαντική αυτή βιοτεχνολογική ανάπτυξη να οδηγήσει σε οικονομικά εφικτές λύσεις. Η παρούσα μελέτη ανταπεξέρχεται στην πρόκληση της εξερεύνησης χαμηλής κατανάλωσης υλοποίησεων της βιοτεχνολογικής αυτής εφαρμογής. Η εφαρμογή έχει αναπτυχθεί σε υπολογιστικό περιβάλλον Matlab και εν συνεχεία ακολουθεί η μετάφρασή της σε C προγραμματιστική γλώσσα. Επιπρόσθετα, εφαρ-μόσθηκε δυναμική ανάλυση του εύρους και ανάλυση της ακρίβειας των μεταβλητών, με στόχο τον προσδιορισμό των μηκών των fixed point λέξεων. Εν κατακλείδι, πραγμα-τοποιήθηκαν platform-independent και platform-dependent μετασχηματισμοί της εφαρμογής και υλοποιείται σε διαφορετικές ASIP αρχιτεκτονικές αποσκοπώντας στην επίτευξη χαμηλής κατανάλωσης ενέργειας.
- Published
- 2009
35. Advances in genome studies: the PAG 2010 conference
- Author
-
Appels, Rudi, Barrero, Roberto, Keeble-Gagnere, Gabriel, Bellgard, Matthew, Appels, Rudi, Barrero, Roberto, Keeble-Gagnere, Gabriel, and Bellgard, Matthew
- Published
- 2010
36. Ultrasonic manipulation in a microfluidic chip for individual handling of particles and cells
- Author
-
Wiklund, Martin, Hultström, Jessica, Manneberg, Otto, Hertz, Hans M., Wiklund, Martin, Hultström, Jessica, Manneberg, Otto, and Hertz, Hans M.
- Abstract
We have developed a microfluidic platform for individual particle handling by the use of ultrasonic standing waves. Elementary manipulation functions, useful in cell-based biotechnology applications, are demonstrated. Oblique coupling of ultrasound allows for any kind of high-NA optical microscopy, which is important for individual characterization of cells., QC 20120525
- Published
- 2006
37. 20.320 Biomolecular Kinetics and Cell Dynamics, Spring 2006
- Author
-
Lauffenburger, Douglas, White, Forest, Lauffenburger, Douglas, and White, Forest
- Abstract
This class covers analysis of kinetics and dynamics of molecular and cellular processes across a hierarchy of scales, including intracellular, extracellular, and cell population levels; a spectrum of biotechnology applications are also taken into consideration. Topics include gene regulation networks; nucleic acid hybridization; signal transduction pathways; and cell populations in tissues and bioreactors. Emphasis is placed on experimental methods, quantitative analysis, and computational modeling.
- Published
- 2006
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.