1. Redistribution of FLAgellar Member 8 during the trypanosome life cycle: Consequences for cell fate prediction
- Author
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Serge Bonnefoy, Brice Rotureau, Audrey Salles, Paul G. McKean, Estefania Calvo Alvarez, Fiona E. Benson, Philippe Bastin, Biologie cellulaire des Trypanosomes - Trypanosome Cell Biology, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), BioImagerie Photonique – Photonic BioImaging (UTechS PBI), Institut Pasteur [Paris], Lancaster University, French National Research Agency, Grant/Award Number: ANR-10-INSB-04, French National Agency for Scientific Research, Grant/Award Numbers: ANR-18-CE15-0012 TrypaDerm, ANR-14-CE14-0019-01 EnTrypa, French Government Investissement d'Avenir Programme, Grant/Award Number: ANR-10-LABX-62-IBEID, Institut National pour la Santé et le Recherche Médicale (INSERM), Institut Pasteur, ANR-18-CE15-0012,TrypaDerm,Comprendre la biologie des trypanosomes africains dans la peau(2018), ANR-14-CE14-0019,ENTRYPA,Dynamique des étapes précoces de l'infection de l'hôte mammifère par les trypanosomes africains(2014), ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), Biologie cellulaire des Trypanosomes, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), and Institut Pasteur [Paris] (IP)
- Subjects
Trypanosoma ,Cell division ,Tsetse Flies ,media_common.quotation_subject ,cells ,[SDV]Life Sciences [q-bio] ,Trypanosoma brucei brucei ,Protozoan Proteins ,elongation ,Insect ,Biology ,Cell fate determination ,Flagellum ,flagellum ,03 medical and health sciences ,Parasite hosting ,Animals ,division ,remodelling ,Trypanosoma brucei ,Cytoskeleton ,Research Articles ,ComputingMilieux_MISCELLANEOUS ,030304 developmental biology ,media_common ,0303 health sciences ,Life Cycle Stages ,030306 microbiology ,urogenital system ,musculoskeletal, neural, and ocular physiology ,Midgut ,Cell Differentiation ,differentiation ,biochemical phenomena, metabolism, and nutrition ,Cell biology ,FLAM8 ,[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology ,Flagella ,bacteria ,Research Article - Abstract
The single flagellum of African trypanosomes is essential in multiple aspects of the parasites' development. The FLAgellar Member 8 protein (FLAM8), localised to the tip of the flagellum in cultured insect forms of Trypanosoma brucei, was identified as a marker of the locking event that controls flagellum length. Here, we investigated whether FLAM8 could also reflect the flagellum maturation state in other parasite cycle stages. We observed that FLAM8 distribution extended along the entire flagellar cytoskeleton in mammalian‐infective forms. Then, a rapid FLAM8 concentration to the distal tip occurs during differentiation into early insect forms, illustrating the remodelling of an existing flagellum. In the tsetse cardia, FLAM8 further localises to the entire length of the new flagellum during an asymmetric division. Strikingly, in parasites dividing in the tsetse midgut and in the salivary glands, the amount and distribution of FLAM8 in the new flagellum were seen to predict the daughter cell fate. We propose and discuss how FLAM8 could be considered a meta‐marker of the flagellum stage and maturation state in trypanosomes., FLAgellar Member 8 protein (FLAM8) was identified as a marker of the locking event that controls flagellum length in African trypanosomes. Here, we show that FLAM8 extends along the entire flagellar cytoskeleton in mammalian infective forms (ST) of T. brucei and concentrates to the tip during differentiation into the insect procyclic stage (PCF). In parasites dividing in the tsetse midgut, the amount and distribution of FLAM8 in the new flagellum predicts the daughter cell fate, either towards another round of proliferation or towards flagellum elongation and next cell differentiation (LT and DE). In total, FLAM8 could be considered as a meta‐marker of the flagellum stage and maturation state in trypanosomes.
- Published
- 2021
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