145 results on '"Biodecolorization"'
Search Results
2. Physicochemical Investigations of Textile Wastewater and Process Parameter Optimization for Biodecolorization of Congo Red Dye by Pseudomonas aeruginosa MT-2 Strain.
- Author
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Tripathi, Manikant, Shukla, Shivpujan, Singh, Ranjan, Singh, Sangram, Singh, Pankaj, Singh, Pradeep Kumar, Shukla, Awadhesh Kumar, Maurya, Sadanand, Pathak, Sukriti, Chaudhary, Vinod Kumar, Shukla, Ajay Kumar, and Manimekalai, R.
- Subjects
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INDUSTRIAL wastes , *CONGO red (Staining dye) , *PSEUDOMONAS aeruginosa , *BIOCHEMICAL oxygen demand , *CHEMICAL oxygen demand - Abstract
Pollution caused by dyes is a major environmental threat, posing adverse impacts on humans, animals, and plants. Therefore, the remediation of such pollutants is essential to protect the environment. This study aimed to conduct physicochemical and bacteriological analyses of textile wastewater to isolate and identify potential native bacterial strains for the decolorization of Congo red dye. Physical and nutritional process parameters were optimized to achieve maximum decolorization. The biological and chemical oxygen demands of the analyzed textile waste water were found to be above the recommended limits. In this study, 19 Congo red -decolorizing bacteria were isolated, with one bacterial culture capable of growing at a higher dye concentration of 300 mg/L. This bacterium was characterized biochemically and genetically (using 16S rRNA sequencing) and identified as the Pseudomonas aeruginosa MT-2 strain. A maximum decolorization of 94.0% was achieved at an initial dye concentration of 150 mg/L, 35°C, and pH 8.0 under static conditions. The bacterial culture also showed resistance to heavy metals such as arsenic, lead, and chromium. The biodegradation of Congo red dye was confirmed through UV-vis spectral analysis and Fourier transform infrared spectrophotometry. The findings of this study demonstrate the high remediation potential of the MT-2 strain, making it suitable for possible use in dye biodecolorization at contaminated sites. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
3. Novel approaches of mycosynthesized zinc oxide nanoparticles (ZnONPs) using Pleurotus sajor-caju extract and their biological and environmental applications.
- Author
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Manimaran, Kumar, Yanto, Dede Heri Yuli, Sari, Ira Puspita, Karimah, Silviyani Nurul, Kamaraj, Chinnaperumal, Manoharadas, Salim, Praburaman, Loganathan, Suganthi, Sanjeevamuthu, and Oh, Tae Hwan
- Abstract
In this study, mycosynthesized zinc oxide nanoparticles (ZnONPs) are fabricated via Pleurotus sajor-caju mushroom extract, and their potential medical and environmental applications are demonstrated. The biosynthesized ZnONPs were assessed for their antibacterial, anticancer, and biodecolorization potential efficiency. They were also characterized and morphologically analyzed by UV-visible spectroscopy, XRD, FT-IR, FE-SEM, EDX, HR-TEM, Zeta potential, and GC-MS analysis. The UV visible spectrum analysis of synthesized ZnONPs analyzed outcome 354 nm was the SPR peak that the nanoparticles displayed. The characteristic Zn–O bond was indicated by a strong peak in the FT-IR study at 432.05 cm
−1 . Based on XRD analysis, P. sajor-caju mediated ZnONPs were crystalline nature, with an average nano particle size of 14.21 nm and a polydispersity directory of 0.29. The nanoparticles exhibit modest constancy, as shown by their zeta potential value of − 33.2 mV. The presence of oxygen and zinc was verified by EDX analysis. The ZnONPs were found to be spherical in shape and crystalline nature structure, with smooth surface morphology and a mean particle size of 10 nm using HR-TEM and SAED analysis. The significant antibacterial activity against S. aureus (6.2 ± 0.1), S. mutans (5.4 ± 0.4), and B. subtilis (5.2 ± 0.1 mm) was demonstrated by the synthesized ZnONPs made using mushroom extract. It was discovered that when the concentration of mushroom extract was increased together with synthesized ZnONPs, the bactericidal activity increased considerably. A higher concentration of ZnONPs demonstrated superior antibacterial activity across the ZnONPs ratio tests. The in vitro cytotoxicity assay showed that ZnONPs, even at low doses, had a substantial number of cytotoxic effects on liver cancer cells (LC50 values 47.42 µg/mL). The effectiveness test revealed that acid blue 129 was degraded. The best decolorization of acid blue 129 at 72.57% after 3 h of soaking serves as evidence for the theory that myco-synthesized ZnONPs by P. sajor-caju mushroom can function as catalysts in reducing the dye. The mycosynthesized ZnONPs from P. sajor-caju extract, and its potential for antibacterial, anticancer, and decolorization are in this investigation. The mycosynthesized ZnONPs suggest a novel use for nanoparticles in the creation of environmental and medicinal products. [ABSTRACT FROM AUTHOR]- Published
- 2024
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- View/download PDF
4. Biodecolorization and Biodegradation of Sulfur Black by the Strain Aspergillus sp. DS-28.
- Author
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Guan, Zhipeng, Wang, Yating, Chen, Wentao, Li, Yanchen, Yue, Wenlong, and Cai, Zhiqiang
- Subjects
LIQUID chromatography-mass spectrometry ,HIGH performance liquid chromatography ,SIZE reduction of materials ,INFRARED spectroscopy ,WASTE management - Abstract
The textile industry significantly contributes to environmental pollution through its use of synthetic dyes, especially sulfur black, known for its toxicity and resistance to degradation. This research focuses on a fungal strain, Aspergillus sp. strain DS-28, isolated from activated sludge, which exhibits an exceptional ability to biodegrade sulfur black dye. This study systematically assessed the biodegradation capacity of this strain through a series of experiments conducted over a 7-day period. Analytical techniques including high-performance liquid chromatography time-of-flight mass spectrometry (HPLC-TOF/MS), scanning electron microscopy (SEM), X-ray diffraction (XRD), and Fourier-transform infrared spectroscopy (FTIR) were employed to monitor the degradation process. SEM showed a significant reduction in particle size, with surfaces becoming smoother and flatter post treatment. XRD indicated a decrease in the intensity of several chemical bonds, and FTIR analysis demonstrated the enhanced vibrational absorption peaks of benzene ring bonds, with the disappearance of -C-S- and -C-S-S-C- groups. The results demonstrate that Aspergillus sp. DS-28 degrades sulfur black by initiating the oxidative breakdown of its complex structures into simpler forms. This study not only elucidates the biodegradation pathway facilitated by Aspergillus sp. DS-28, but also highlights its potential application in developing eco-friendly waste management strategies for treating dye-contaminated wastewater. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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5. The effect of Ralstonia pickettii addition on methylene blue dye biodecolorization by brown-rot fungus Gloeophyllum trabeum
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Adi Setyo Purnomo, Badzlin Nabilah, Hilda Mahfudhah, Hamdan Dwi Rizqi, Surya Rosa Putra, and Yuji Tsutsumi
- Subjects
Biodecolorization ,Gloeophyllum trabeum ,Methylene blue ,Mixed culture ,Ralstonia pickettii, pollutants ,Chemical engineering ,TP155-156 - Abstract
Methylene Blue (MB) is a thiazine group dye frequently used in the textile industry but the difficulty in degrading its molecule poses a significant risk of toxicity to humans. Gloeophyllum trabeum, a brown-rot fungus, has been previously shown to degrade MB. However, the decolorization capacity achieved was relatively poor due to the extended incubation time. This study aimed to improve the MB degradation process by G. trabeum with the addition of Ralstonia pickettii bacteria. The concentrations of R. pickettii added included 2, 4, 6, 8, and 10 mL (1 mL ≈ 1.39 × 108 CFU), while the degradation process was conducted at 30 °C within a 7-day incubation period. The results showed that the highest decolorization percentage was obtained with the addition of 10 mL R. pickettii. The mixed cultures decolorized MB by approximately 85%, while G. trabeum achieved 11% decolorization. The metabolite product produced from the process included C12H13N3O6, C14H14N3S, C12H11N3SO6, C12H11N3SO7, and C22H15N3SO5. Therefore, it was concluded that R. pickettii could enhance the capability of G. trabeum to decolorize MB.
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- 2024
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6. Biodecolorization and biodegradation of Reactive Green 12 textile industry dye and their post-degradation phytotoxicity-genotoxicity assessments.
- Author
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Singh, Shweta, Gautam, Roshan Lal, Chaudhary, Dhirendra K., Singh, Devendra, and Naraian, Ram
- Abstract
The employment of versatile bacterial strains for the efficient degradation of carcinogenic textile dyes is a sustainable technology of bioremediation for a neat, clean, and evergreen globe. The present study has explored the eco-friendly degradation of complex Reactive Green 12 azo dye to its non-toxic metabolites for safe disposal in an open environment. The bacterial degradation was performed with the variable concentrations (50, 100, 200, 400, and 500 mg/L) of Reactive Green 12 dye. The degradation and toxicity of the dye were validated by high-performance liquid chromatography, Fourier infrared spectroscopy analysis, and phytotoxicity and genotoxicity assay, respectively. The highest 97.8% decolorization was achieved within 12 h. Alternations in the peaks and retentions, thus, along with modifications in the functional groups and chemical bonds, confirmed the degradation of Reactive Green 12. The disappearance of a major peak at 1450 cm−1 corresponding to the –N=N– azo link validated the breaking of azo bonds and degradation of the parent dye. The 100% germination of Triticum aestivum seed and healthy growth of plants verified the lost toxicity of degraded dye. Moreover, the chromosomal aberration of Allium cepa root cell treatment also validated the removal of toxicity through bacterial degradation. Thereafter, for efficient degradation of textile dye, the bacterium is recommended for adaptation to the sustainable degradation of dye and wastewater for further application of degraded metabolites in crop irrigation for sustainable agriculture. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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7. Eco-friendly treatment of synthetic dyes contaminated water by biosorption: use of Bacillus mojavensis BI2 derived lipopeptide and date palm waste flour as biosorbents.
- Author
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Mnif, Inès, Mekki, Salwa, and Ghribi, Dhouha
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WATER use , *PALMS , *BACILLUS (Bacteria) , *DATE palm , *COLOR removal (Sewage purification) , *MALACHITE green , *GENTIAN violet - Abstract
In the former section of the present paper, the prospective application of B. mojavensis BI2 derived lipopeptides for dyes biodecolorization was demonstrated. The lipopeptide was mainly composed of linear and cyclic Fengycin and Surfactin isoforms. When using 100 mg/L, we are able to remove about 98% of 100 and 200 mg/L Malachite Green (MG). For the Crystal Violet (CV), the % of biodecolorization does not exceed 85% in the presence of 100 and 400 mg/L BI2 lipopeptide. In the second part of the paper, we proved the efficiency of dyes biosorption by palm waste floor, optimized by a Taguichi design. The bio-treatment efficiency was evaluated by color removal and the enhancement of germination index. Optimum treatments were obtained with 10 g/L and 6 g/L palm waste for 200 mg/L MG and CV respectively during 1 hour. Nevertheless, BI2 lipopeptide addition has no significant effect on color biosorption by date palm waste flour. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
8. Mycoremediation of Synthetic Azo Dyes by White-Rot Fungi Grown on Diary Waste: A Step toward Sustainable and Circular Bioeconomy.
- Author
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Gugel, Irene, Summa, Daniela, Costa, Stefania, Manfredini, Stefano, Vertuani, Silvia, Marchetti, Filippo, and Tamburini, Elena
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FUNGAL remediation ,SUSTAINABLE development ,MANGANESE peroxidase ,AZO dyes ,DAIRY waste ,ENDOCRINE disruptors ,LIGNIN structure ,COLOR removal (Sewage purification) - Abstract
This study assesses the efficacy of three white-rot fungi—Bjerkandera adusta, Phanerochaete chrysosporium, and Trametes versicolor—in degrading synthetic dyes and lignin in pulp and paper mill effluents, which annually contribute around 40,000 million cubic meters of dyed waste. Exploiting the structural resemblance of dyes to lignin, the fungi utilize ligninolytic enzymes—lignin peroxidase, manganese peroxidase, and laccase—to break down the pollutants. Initial mycoremediation trials in synthetic dye solutions with Direct black 80, Direct yellow 11, Basic brown 1, Orange II, and Red 8 BLP achieved decolorization rates of 70–80% within 7 days, except for Red 8 BLP. Both soluble and insoluble lignin fractions were significantly reduced, with an overall removal rate of 80–90%. Contrary to prior beliefs about the recalcitrance of azo dyes, B. adusta demonstrated substantial biodegradation capabilities, even on non-lignocellulosic substrates, such as dairy waste. The decolorization efficacy varied with dye structure, suggesting that efficiency should not be judged solely on color reduction. Remarkably, B. adusta also effectively decolorized and removed lignin from actual mill effluents without pH alteration, indicating a viable low-cost bioremediation strategy. This invites further investigation into optimizing B. adusta for industrial wastewater biodecolorization, especially in the field of PAHs (Polycyclic Aromatic Hydrocarbons) and EDCs (Endocrine Disrupting Chemicals). [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
9. Optimization of laccase from Stenotrophomonas maltophilia E1 by submerge fermentation using coconut husk with its detoxification and biodecolorization ability of synthetic dyes
- Author
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Yazeed Albulaihed, Mohd Adnan, Arshad Jamal, Mejdi Snoussi, Kartik Patel, and Mitesh Patel
- Subjects
Stenotrophomonas maltophilia ,Biodecolorization ,Detoxification ,Synthetic dyes ,Phytotoxicity ,Laccase ,Technology ,Chemical technology ,TP1-1185 ,Biotechnology ,TP248.13-248.65 - Abstract
Abstract Enzymatic degradation of synthetic dyes holds an immense promise for addressing the environmental concerns associated with the textile and dye industries. This study aimed to isolate bacteria capable of producing laccase enzymes from an anthropogenic environment. Subsequently, viability of utilizing cost-effective agricultural residues as substrates for laccase production was assessed. Response Surface Methodology (RSM) and the One Variable at a Time (OVAT) approach was pursued for the optimization of laccase production, followed by pH and temperature stability, dye degradation and decolorization experiments, toxicological studies on the degraded dye metabolites. In results, laccase-producing bacterial strain was identified as Stenotrophomonas maltophilia strain E1 (S. maltophilia). Among variety of substrates, coconut husk exhibited optimal efficacy. In a statistical optimization study, it was found that S. maltophilia was capable of producing laccase 51.38 IU/mL, i.e., three times higher than the amount of laccase produced by unoptimized medium (16.7 IU/mL), and the enzyme activity was found to be steady at an acidic pH, and a mesophilic temperature range. The laccase obtained from S. maltophilia E1 demonstrated proficient dye decolorization capabilities, achieving a notable 92.1% reduction in Malachite green dye coloration at a concentration of 500 ppm. Gas chromatography–mass spectrometry (GC–MS) analysis of the decolorized derivatives of Malachite green revealed a conversion into a distinct compounds. Moreover, after undergoing laccase treatment, Malachite green exhibited decreased phytotoxic effects on Oryza sativa, pointing to enzymatic detoxification. Collectively, insights gained from the present study will contribute to the development of efficient enzymatic approaches for addressing the environmental pollution caused by synthetic dyes. Graphical Abstract
- Published
- 2023
- Full Text
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10. Optimization of laccase from Stenotrophomonas maltophilia E1 by submerge fermentation using coconut husk with its detoxification and biodecolorization ability of synthetic dyes.
- Author
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Albulaihed, Yazeed, Adnan, Mohd, Jamal, Arshad, Snoussi, Mejdi, Patel, Kartik, and Patel, Mitesh
- Subjects
LACCASE ,STENOTROPHOMONAS maltophilia ,GAS chromatography/Mass spectrometry (GC-MS) ,AGRICULTURAL wastes ,RESPONSE surfaces (Statistics) ,PHYTOTOXICITY ,MALACHITE green - Abstract
Enzymatic degradation of synthetic dyes holds an immense promise for addressing the environmental concerns associated with the textile and dye industries. This study aimed to isolate bacteria capable of producing laccase enzymes from an anthropogenic environment. Subsequently, viability of utilizing cost-effective agricultural residues as substrates for laccase production was assessed. Response Surface Methodology (RSM) and the One Variable at a Time (OVAT) approach was pursued for the optimization of laccase production, followed by pH and temperature stability, dye degradation and decolorization experiments, toxicological studies on the degraded dye metabolites. In results, laccase-producing bacterial strain was identified as Stenotrophomonas maltophilia strain E1 (S. maltophilia). Among variety of substrates, coconut husk exhibited optimal efficacy. In a statistical optimization study, it was found that S. maltophilia was capable of producing laccase 51.38 IU/mL, i.e., three times higher than the amount of laccase produced by unoptimized medium (16.7 IU/mL), and the enzyme activity was found to be steady at an acidic pH, and a mesophilic temperature range. The laccase obtained from S. maltophilia E1 demonstrated proficient dye decolorization capabilities, achieving a notable 92.1% reduction in Malachite green dye coloration at a concentration of 500 ppm. Gas chromatography–mass spectrometry (GC–MS) analysis of the decolorized derivatives of Malachite green revealed a conversion into a distinct compounds. Moreover, after undergoing laccase treatment, Malachite green exhibited decreased phytotoxic effects on Oryza sativa, pointing to enzymatic detoxification. Collectively, insights gained from the present study will contribute to the development of efficient enzymatic approaches for addressing the environmental pollution caused by synthetic dyes. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
11. Analysis of Batch Kinetic Data of Biodecolorization Reaction: Theoretical Approach for the Design of Packed Bed Reactor.
- Author
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Rethinam, Brindha, Palanichamy, Rajaguru, and John Britto, Jennet Debora
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PACKED bed reactors , *AZO dyes , *WASTEWATER treatment , *SUSTAINABLE development , *BACILLUS cereus , *DYE-sensitized solar cells , *COLOR removal in water purification , *ANAEROBIC reactors - Abstract
The degradation of azo dyes by conventional methods has proven ineffective due to their complex structure and synthetic nature. Bioremediation of azo dye containing textile wastewater requires an appropriate selection of potential strains to address its potential hazards. This study focuses on the biodegradation of azo dyes containing textile wastewater by isolating halotolerant bacterial strains from marine coastal soil. The rapid degradation of model dye Mordant Yellow 10 (MY10) was monitored spectrophotometrically and it was found that decolorization of MY10 by isolated strains Bacillus firmus (BA01), Pseudomonas aeroginosa (BRPO3), and Bacillus cereus (BRPO4) and mixed consortium CMBS follows zero-, second-, first-, and one-and-a-half-order kinetics, respectively. Through the batch kinetic analysis of MY10 degradation, it was observed that bacterial strain Pseudomonas aeroginosa BRPO3 was more effective with reaction rate constant and half-life as 1,024±213×10−2 mM−1 day−1 and 0.3±0.1 day , respectively. Further kinetic analysis using BRPO3 helps describe that initial dye and glucose concentration were the driving forces of the dye degradation reaction and thus were used to construct kinetic rate equation. Using a stepwise protocol, this kinetic model was applied to calculate the design parameters including optimal height and working volume of an upflow anaerobic packed bed (UAPB) reactor as 0.95 m and 4.78 L, respectively, for 85% dye conversion. Design was validated by evaluating the performance of the newly fabricated UAPB reactor for treatment of simulated wastewater containing MY10 and real textile wastewater. Complete dye removal and 94.5% total organic carbon (TOC) reduction were observed with simulated wastewater, whereas in real textile effluent 82.5% dye removal and 89% TOC reduction were achieved. Thus, the predicted results corresponded satisfactorily with the experimental data in both wastewater treatments. This study highlights the usefulness of analyzing biodegradation kinetics to improve both the construction of microbial consortia and the development of reactors for wastewater treatments in the context of bioeconomy. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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12. Optimization of textile effluent bacterial treatment and improvement of the process efficiency through SPB1 biosurfactant addition
- Author
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Inès Mnif, Mouna Bouassida, Lamya Ayed, and Dhouha Ghribi
- Subjects
biodecolorization ,box–bhenken ,cod decrease ,response surface methodology ,textile effluent ,Environmental technology. Sanitary engineering ,TD1-1066 - Abstract
The current work aims to optimize biological textile effluent treatment through the use of newly selected bacterial consortia composed of two strains: Citrobacter sedlakii RI11 and Aeromonas veronii GRI. We assessed the effect of SPB1 biosurfactant addition on color removal (CR). The process was optimized by a Box–Bhenken by examining the effect of pH, consortia density and biosurfactant value on treatment efficiency. Firstly, physicochemical analyses of the studied effluent revealed an alkaline pH along with a high content of suspended materials and large amounts of organic matter. Optimal CR and a chemical oxygen demand abatement of about 94 and 86% were obtained when treating the textile effluent at pH 5 with a total optical density of 0.4 and by incorporating 0.01% SPB1 biosurfactant. Additionally, an abolishment of phytotoxicity was registered after treatment optimization. The evaluations of the action mode of both selected bacteria during textile effluent treatment suggested the occurrence of biodegradation phenomena of dyes through enzymatic activities. HIGHLIGHTS Bacterial textile effluent treatment.; Optimization of the treatment by Box–Bhenken design and response surface methodology.; Optimal treatment by the increase of color removal and the abatement of chemical oxygen demand.; Enhancement of the treatment by SPB1 biosurfactant addition.;
- Published
- 2023
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13. Biotransformation of Methylene Blue by Mixed Fungal Cultures of Gloeophyllum trabeum and Aspergillus oryzae
- Author
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Adi Setyo Purnomo, Adelia Sabilah Prameswari, Hamdan Dwi Rizqi, Taufiq Rinda Alkas, Ratna Ediati, and Yuly Kusumawati
- Subjects
aspergillus oryzae ,biodecolorization ,gloeophyllum trabeum ,methylene blue ,mixed culture ,pollutants ,Technology ,Technology (General) ,T1-995 - Abstract
This study investigated the biotransformation of methylene blue (MB) by mixed fungal cultures of Gloeophyllum trabeum and Aspergillus oryzae. Equal volumes of A. oryzae and G. trabeum cultures were applied to Erlenmeyer containing MB and incubated at 30°C for 7 days. The change in absorbance of the MB control solution and the solution after application, measured with a UV-Visible spectrophotometer, was used to calculate the decolorization. The addition of A. oryzae to G. trabeum cultures showed MB biodecolorization reaching 69.34%, greater than single cultures of G. trabeum and A. oryzae, which were 31.50% and 36.82%, respectively. Metabolite identification from MB biodecolorization by mixed culture using LC-QTOF-MS found the following metabolites: C16H20N3S, C19H22N3SO4, C31H48N3S+. The results of this study showed that the addition of A. oryzae enhanced the percentage of MB decolorization from G. trabeum culture.
- Published
- 2022
- Full Text
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14. Decolorization of methyl red using Bacillus thuringiensis RI16 strain: Enhanced bacterial treatment by SPB1 biosurfactant addition
- Author
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Ines Mnif, Raouia Fendri, and Dhouha Ghribi
- Subjects
bacillus thuringiensis ,biodecolorization ,biosurfactants ,methyl red ,Environmental technology. Sanitary engineering ,TD1-1066 - Abstract
In this work, we evaluate the ability of a novel isolated bacteria identified as Bacillus thuringiensis RI16 (KM111604) to decolorize methyl red. Results showed its efficiency for the treatment of azo-dye-contaminated wastewaters under static conditions at pH values ranging from 7 to 9. The isolated bacteria could tolerate higher doses of dyes as it was able to decolorize up to 1000 mg/L. For better methyl red decolorization, we evaluate the potential use of Bacillus subtilis SPB1-derived lipopeptide biosurfactant. The study showed its ability to accelerate the decolorization rate and slightly maximized the decolorization efficiency at an optimal concentration of 0.025%. Compared with synthetic surfactants, the bacterial surfactant was more efficient as we observe a drastic decrease and an inhibition of methyl red decolorization by cetyl trimethylammonium bromide (CTAB) and sodium dodecyl sulfate (SDS) additions. Also, studies ensured that methyl red removal by this strain could be due to extracellular enzymatic activities. SPB1 biosurfactant could improve enzyme activities and/or enzyme synthesis and diffusion. HIGHLIGHTS B. thuringiensis RI16 for methyl red biodecolorization under static conditions and neutral and alkaline pH values.; B. subtilis SPB1-derived lipopeptide-enhanced methyl red decolorization.; Inhibition of methyl red decolorization by CTAB and SDS additions.; Tween 80 enhanced methyl red decolorization.; Improvement of color removal-related enzymes’ activities and/or enzymes’ synthesis and diffusion by SPB1 biosurfactant.;
- Published
- 2022
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15. Methylene Blue biodecolorization and biodegradation by immobilized mixed cultures of Trichoderma viride and Ralstonia pickettii into SA-PVA-Bentonite matrix
- Author
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Badzlin Nabilah, Adi Setyo Purnomo, Didik Prasetyoko, and Alya Awinatul Rohmah
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Methylene Blue ,Biodecolorization ,Ralstonia pickettii ,Trichoderma viride ,Immobilization ,Pollution ,Chemistry ,QD1-999 - Abstract
The problem of industrial dye wastewater poses a critical environmental challenge that demands urgent attention. This is because the direct release of synthetic dyes such as Methylene Blue (MB) into water bodies has been found to have adverse effects on the environment. Therefore, this study aimed to propose immobilization of a mixed Trichoderma viride and Ralstonia pickettii culture into Sodium alginate–Polyvinyl Alcohol-Bentonite (SA-PVA-Bentonite) matrix as a development method for MB decolorization and degradation. Immobilization process was carried out using the entrapment method, where bacteria and fungi cells were homogenized into the SA-PVA-Bentonite matrix. The results showed that immobilized culture (IMO Mix) outperformed the free cells in Mineral Salt Medium (MSM), achieving an impressive 97.88% decolorization rate for 48 h at 30 °C. Furthermore, a total of 3 metabolite product degradation were produced including Azure A and C, as well as Thionine by LCMS analysis. SEM-EDX analysis confirmed that culture was agglomerated within the SA-PVA-Bentonite matrix, while FTIR demonstrated the functional groups of the synthesized beads. Meanwhile, the difference in charge of bentonite facilitated the adsorption of MB onto the beads, and mixed culture supported the degradation process. This study presented a potential solution to environmental problems, particularly those related to the industrial sector. Further analysis was required to address the challenges associated with other industrial dye waste.
- Published
- 2023
- Full Text
- View/download PDF
16. Evaluation of the adsorptive removal of cationic dyes by greening biochar derived from agricultural bio-waste of rice husk.
- Author
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Saravanan, Praveen, Josephraj, Jegan, Thillainayagam, Bhagavathi Pushpa, and Ravindiran, Gokulan
- Abstract
In this study, biochar derived from agricultural bio-wastes of rice husk was used as a biosorbent for dye sequestration of Basic Blue 41 (BB41) and Basic Red 09 (BR09) from the aqueous solution. The structural characteristics of the biochar were performed through TGA, BET, SEM, EDX, and FTIR. The experimental conditions proved that 80% of dye removal was attained in the optimal operating conditions (dosage of 6 g L
−1 (BB41), 1 g L−1 (BR09), pH of 7 (BB41) and 8 (BR09), temperature of 35 °C, and initial dye concentration of 50 mg L−1 ). Four different isotherms models were used to describe the adsorption process. Based on the correlation coefficient and percentage of error values, BB41 and BR09 sorption revealed that Langmuir model has better fit when compared to other models. The maximum adsorption capacities of BB41 and BR09 on rice husk biochar are 17.596 mg g−1 and 168.49 mg g−1 , respectively. The thermodynamic parameters △H < 0 and △G < 0 indicated that the whole adsorption process of rice husk biochar to BB41 and BR09 is exothermic and spontaneous at low temperature. The regeneration studies were carried out with different elutants to confirm the elution efficiency of the sorbent. The results indicate the biochar derived from agricultural bio-waste of rice husk was a potentially greening biosorbent for the dye sequestration from wastewater. [ABSTRACT FROM AUTHOR]- Published
- 2023
- Full Text
- View/download PDF
17. Mycoremediation of Synthetic Azo Dyes by White-Rot Fungi Grown on Diary Waste: A Step toward Sustainable and Circular Bioeconomy
- Author
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Irene Gugel, Daniela Summa, Stefania Costa, Stefano Manfredini, Silvia Vertuani, Filippo Marchetti, and Elena Tamburini
- Subjects
mycoremediation ,biodecolorization ,white-rot fungi ,Bjerkandera adusta ,Phanerochete chrysosporium ,Trametes versicolor ,Fermentation industries. Beverages. Alcohol ,TP500-660 - Abstract
This study assesses the efficacy of three white-rot fungi—Bjerkandera adusta, Phanerochaete chrysosporium, and Trametes versicolor—in degrading synthetic dyes and lignin in pulp and paper mill effluents, which annually contribute around 40,000 million cubic meters of dyed waste. Exploiting the structural resemblance of dyes to lignin, the fungi utilize ligninolytic enzymes—lignin peroxidase, manganese peroxidase, and laccase—to break down the pollutants. Initial mycoremediation trials in synthetic dye solutions with Direct black 80, Direct yellow 11, Basic brown 1, Orange II, and Red 8 BLP achieved decolorization rates of 70–80% within 7 days, except for Red 8 BLP. Both soluble and insoluble lignin fractions were significantly reduced, with an overall removal rate of 80–90%. Contrary to prior beliefs about the recalcitrance of azo dyes, B. adusta demonstrated substantial biodegradation capabilities, even on non-lignocellulosic substrates, such as dairy waste. The decolorization efficacy varied with dye structure, suggesting that efficiency should not be judged solely on color reduction. Remarkably, B. adusta also effectively decolorized and removed lignin from actual mill effluents without pH alteration, indicating a viable low-cost bioremediation strategy. This invites further investigation into optimizing B. adusta for industrial wastewater biodecolorization, especially in the field of PAHs (Polycyclic Aromatic Hydrocarbons) and EDCs (Endocrine Disrupting Chemicals).
- Published
- 2024
- Full Text
- View/download PDF
18. Decolorization and Transformation of Synthetic Dye Methylene Blue by Brown-Rot Fungus Fomitopsis pinicola
- Author
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Adi Setyo Purnomo, Hamdan Dwi Rizqi, Aulia Ulfi, Refdinal Nawfa, and Herdayanto Sulistyo Putro
- Subjects
biodegradation ,biodecolorization ,fomitopsis pinicola ,brown-rot fungi ,methylene blue ,Chemistry ,QD1-999 - Abstract
Methylene blue (MB) is a synthetic dye widely used in industries that is difficult to degrade in the environment due to its stability. Brown-rot fungus Fomitopsis pinicola is an organism that is known to be able to degrade some organic pollutants such as DDT, aldrin, dieldrin, and methyl orange dye. This study aimed to explore the ability of F. pinicola on MB biodecolorization, to identify metabolites and propose a biodecolorization pathway. F. pinicola was evaluated for MB biodecolorization on PDA agar and PDB liquid media. The metabolites were determined by using LCTOF/MS. The PDA agar medium's largest index decolorization (ID) was 0.915%. The MB decolorization in liquid PDB medium showed the highest percentage of decolorization of 92.56% at MB concentration of 100 mg/L after 14-days incubation. The analysis using LC-TOF/MS showed metabolites from MB biodecolorization, namely 3-amino-7-(methylamino) phenothiazin-5-ium (Azure C), 3-(dimethylamino)-7-(methylamino) phenothiazin-5-ium (Azure B), and 3,7-bis(dimethylamino)-4aH-phenothiazin-5-one. The MB degradation pathway was proposed through demethylation and oxidation reactions based on the detected product metabolites. These results indicated that F. pinicola is a suitable agent for biodecolorization of MB, and can potentially be used for bioremediation of MB waste in the environment.
- Published
- 2022
- Full Text
- View/download PDF
19. A Green Biocomposite Produced by Passive Cell Immobilization onto Waste Biomass Support for Biodecolorization of Reactive Dye Contamination.
- Author
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Celik, Sema
- Subjects
- *
REACTIVE dyes , *BIOMASS , *CIRCULAR economy , *WASTE recycling , *WASTEWATER treatment , *COLOR removal (Sewage purification) - Abstract
The waste biomass of grape pomace was utilized for the immobilization of model fungal cells (Thamnidium elegans) providing a green, innovative, and practical biocomposite for efficient biodecolorization of a model reactive dye (Reactive Yellow 2). Appreciable decolorization capacity at optimized conditions was observed as qmax=151.0 mg g‐1 with the suggested biocomposite (TECWGP). During the batch process, the biocomposite exhibited the highest dye removal of 98.57 % in the RY2‐contaminated solutions. The biosorptive decolorization process was the best predicted by the Langmuir isotherm and the pseudo‐second‐order kinetic models. The possible interactions between RY2 and TECWGP were also examined. Synthetic wastewater treatment experiments indicated that TECWGP has a good decolorization potential. Overall, the findings indicated that TECWGP could be a good alternative to synthetic materials for the treatment of reactive dye‐contaminated aqueous phase. Furthermore, the utilization of agro‐industrial waste to support fungal cells contributes to the growth of a solid circular bio‐based economy. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
20. Biotransformation of Methylene Blue by Mixed Fungal Cultures of Gloeophyllum trabeum and Aspergillus oryzae.
- Author
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Purnomo, Adi Setyo, Prameswari, Adelia Sabilah, Rizqi, Hamdan Dwi, Alkas, Taufiq Rinda, Ediati, Ratna, and Kusumawati, Yuly
- Subjects
KOJI ,FUNGAL cultures ,BIOCONVERSION ,BIOTRANSFORMATION (Metabolism) ,SOLID-state fermentation ,METHYLENE blue - Abstract
This study investigated the biotransformation of methylene blue (MB) by mixed fungal cultures of Gloeophyllum trabeum and Aspergillus oryzae. Equal volumes of A. oryzae and G. trabeum cultures were applied to Erlenmeyer containing MB and incubated at 30°C for 7 days. The change in absorbance of the MB control solution and the solution after application, measured with a UVVisible spectrophotometer, was used to calculate the decolorization. The addition of A. oryzae to G. trabeum cultures showed MB biodecolorization reaching 69.34%, greater than single cultures of G. trabeum and A. oryzae, which were 31.50% and 36.82%, respectively. Metabolite identification from MB biodecolorization by mixed culture using LC-QTOF-MS found the following metabolites: C
16 H20 N3 S, C19 H22 N3 SO4 , C31 H48 N3 S+. The results of this study showed that the addition of A. oryzae enhanced the percentage of MB decolorization from G. trabeum culture. [ABSTRACT FROM AUTHOR]- Published
- 2022
- Full Text
- View/download PDF
21. Decolorization of Synthetic Dyes by Tropical Fungi Isolated from Taman Eden 100, Toba Samosir, North Sumatra, Indonesia.
- Author
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Anita, Sita Heris, Ardiati, Fenny Clara, Ramadhan, Kharisma Panji, Budi Laksana, Raden Permana, Sari, Fahriya Puspita, Nurhayat, Oktan Dwi, and Yanto, Dede Heri Yuli
- Subjects
- *
LACCASE , *WASTE treatment , *DYES & dyeing , *FUNGI - Abstract
Taman Eden 100 is one of the tourist parks located in Toba Samosir, North Sumatra, Indonesia that provides a wide range of biodiversity. The objective of this research was to obtain the potential fungal isolate with high laccase activity to decolorize synthetic dyes. The results show that six isolates of Perenniporia subtephropora EDN 050, Trametes hirsuta EDN 082, T. hirsuta EDN 084, T. hirsuta EDN 085, Deconica coprophila EDN 114, and T. pavonia EDN 134, which were confirmed by molecular identification using 5.8 rDNA/ITS analysis, exhibited decolorization activity. Six potential isolates showed the ability to decolorize textile dyes of Acid Blue 129, RBBR,Orange II, and Reactive Black 5. The highest decolorization rate of 100 ppm Acid Blue 129, RBBR, Orange II, and Reactive Black 5 dyes by using T. pavonia EDN 134 were 98.87%, 98.26%, 100%, and 98.11%, respectively after 96 h incubation. Almost all positive isolates also decolorized dyes at higher concentrations (1,000 ppm). This study offers the potential of Indonesian tropical fungal isolates for synthetic dyes waste treatment. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
22. The effect of Ralstonia pickettii bacterium addition on methylene blue dye biodecolorization by brown-rot fungus Daedalea dickinsii
- Author
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Badzlin Nabilah, Adi Setyo Purnomo, Hamdan Dwi Rizqi, Herdayanto Sulistyo Putro, and Refdinal Nawfa
- Subjects
Biodecolorization ,Methylene blue ,Mixed cultures ,Daedalea dickinsii ,Ralstonia picketti ,Science (General) ,Q1-390 ,Social sciences (General) ,H1-99 - Abstract
Methylene blue (MB) is one of synthetic dyes that is used in the textile industry which is difficult to degrade in nature. Previously, the brown-rot fungus (BRF) Daedalea dickinsii had shown a good ability to degrade MB, however, the decolorization ability was relatively still low and had a long period of incubation. Therefore, improvement of process is needed to increase the ability of D. dickinsii to decolorize MB. In this study, the effect of Ralstonia pickettii bacterium addition on MB biodecolorization by the BRF D. dickinsii in potato dextrose broth (PDB) medium was investigated. The amount of R. picketti that was added to the culture of D. dickinsii were 2, 4, 6, 8, and 10 mL (1 mL ≈ 1.39 × 108 CFU). The cultures had ability to decolorize MB (100 mg/L) at 30 °C after 7 days incubation. The highest percentage of MB biodecolorization was obtained at addition of 10 mL of R. pickettii approximately 89%, while biodecolorization process by particularly D. dickinsii was approximately 17%. The MB degradation metabolites by mixed cultures of D. dickinsii and 10 mL of R. pickettii were Azure A, thionine, glucose-MB, C12H11N3SO6 and C12H13N3O6. This study indicated that the addition of R. pickettii could enhance MB biodecolorization by fungus D. dickinsii. Besides that, this study also indicated that mixed cultures of D. dickinsii and R. pickettii has great potential for high efficiency, fast and cheap dye wastewater treatment.
- Published
- 2022
- Full Text
- View/download PDF
23. Dye degradation potential of Acinetobacter baumannii strain VITVB against commercial azo dyes.
- Author
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Sreedharan, Veena, Saha, Purbasha, and Rao, Kokati Venkata Bhaskara
- Subjects
- *
AZO dyes , *ACINETOBACTER baumannii , *HIGH performance liquid chromatography , *PHENOTYPIC plasticity , *CHICKPEA , *TEXTILE dyeing - Abstract
The textile industry is an age-old industry in human civilization and to date, it contributes to a major portion of the economy in several nations. However, the generation of a huge volume of toxic colored effluent is a major environmental concern. Azo dyes are extensively used for textile dyeing and are thus found in the wastewater. In this article, bacterial bioremediation of azo dyes was studied. Acinetobacter baumannii strain VITVB, a gram-negative bacterium aerobically decolorized 500 mg L−1 of two textile azo dyes Reactive Blue 221 (RB-221) and Reactive Black 5 (RB-5) with 90% and 87% efficiency after 48 h under static condition. Dye degradation was further maximized by optimization studies. A. baumanii VITVB exerted maximum dye degradation within 24 h under optimized conditions of pH 9, temperature at 45 °C, 5% NaCl concentration, and supplementation of 1% starch (Carbon source) and 1% peptone (Nitrogen source) in growth media. Dye Degradation was then analyzed. Fourier transform infrared spectra of pure and degraded dyes revealed that the potential bacterial strain targeted the characteristic chromophore group (N = N) during the break down of dyes. High-performance liquid chromatography of pure and degraded dyes presented the emergence of different peaks at altered retention times, which also indicates the degradation of dyes. Gas Chromatography–Mass Spectrometry chromatogram revealed probable end products as diphenylmethane from RB-221 degradation and benzene,1,1'-Pentylidenebis from RB-5 degradation. Finally, phytotoxicity and biotoxicity assays implied the nontoxicity of the degraded metabolites of RB-221 and RB-5. Pure dyes inhibited seed germination, showed marked phenotypic changes and cellular damages in plants (Cicer arietinum and Tagetes erecta) while the degraded metabolites promoted a healthy seed germination rate and no marked cellular damages or phenotypic changes in plants. Artemia salina cysts also showed a similar pattern as major hatchability inhibition occurred with pure dyes compared to degraded dye metabolites. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
24. Decolorization of Synthetic Dyes by Ligninolytic Enzymes from Trametes hirsuta D7
- Author
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Sita Heris Anita, Fahriya Puspita Sari, and Dede Heri Yuli Yanto
- Subjects
biodecolorization ,bioremediation ,dye ,ligninolytic enzyme ,textile waste ,Science (General) ,Q1-390 - Abstract
The ability of ligninolytic enzymes from Trametes hirsuta D7 to decolorize several synthetic dyes was investigated. A crude enzyme powder was produced by fermenting oil palm empty fruit bunch fibers for one month. The dye decolorization by the enzymes occurred at an efficient 0.25 U/mL. The enzymes degraded 100, 200, 300, 400, and 500 ppm Remazol Brilliant Blue R (RBBR) within 7 h by 95.57 ± 0.32%, 93.46 ± 3.09%, 91.84 ± 0.65%, 86.44 ± 0.97%, and 82.14 ± 0.52%, respectively. The enzyme also decolorized anthraquinone (Acid Blue 129), monoazo (Acid Orange 7), diazo (Reactive Black 5), and trimethyl methane (Methyl Violet) dyes within 7 h by 94.59 ± 7.97%, 13.99 ± 0.30%, 7.61 ± 0.01%, and 7.59 ± 0.18%, respectively. Addition of MnSO4, H2O2, and violuric acid enhanced the dye decolorization rate by up to 10-fold. This study shows the potential for application of ligninolytic enzymes from T. hirsuta D7 in the treatment of wastewater effluent of textile industries.
- Published
- 2019
- Full Text
- View/download PDF
25. Assessment of biodecolorization potentials of biofilm forming bacteria from two different genera for Mordant Black 11 dye.
- Author
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Tahir, Uruj, Nawaz, Shiza, Hassan Khan, Umair, and Yasmin, Azra
- Subjects
- *
NATURAL dyes & dyeing , *AZO dyes , *BIOFILMS , *BACILLUS subtilis , *BIOCONVERSION , *ULTRAVIOLET-visible spectroscopy , *DYES & dyeing , *HIGH performance liquid chromatography - Abstract
Potential of indigenous biofilm forming Staphylococcus and Bacillus sp. to decolorize and transform Mordant Black 11 dye was exploited for the reclamation of contaminated environmental compartments. The dye decolorization potential of the bacterial strains was assessed against variable environmental parameters. Both the strains exhibited > 50% decolorization of dye in the absence as well as presence of carbon source over 72 h. Bacillus subtilis MB378 was the most potential and efficient strain which exhibited 75.23% decolorization of Mordant Black 11 dye at 37 ˚C within 48 h in glucose supplemented medium. Optimum decolorization of Mordant Black 11 dye by Bacillus subtilis MB378 under aerobic conditions was attained at pH 8 and 30 ˚C within 24 h of incubation. The bacterial strain also possessed the ability to tolerate high concentrations of Mordant Black 11 dye up to 150 mg L−1. Quantitative assessment revealed maximum decolorization of Mordant Black 11 dye (76.12%) by Staphylococcus sp. MB377 at acidic pH, 37 ˚C with 1% inoculum size. Mordant Black 11 dye on addition of metals followed the dye decolorization order as Cd > Cu > Cr. IR analysis, UV–vis spectroscopy, HPLC and GCMS profiles ascertained the biodecolorization and biotransformation potential of these strains supporting effective utilization of indigenous biofilm forming bacteria for comprehensive treatment of dye containing effluents. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
26. A novel microbial consortium from sheep compost for decolorization and degradation of Congo red
- Author
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Sh.F. Abd El-Kader, G.A. El-Chaghaby, G.M. Khalafalla, R.I. Refae, and H.M. Elshishtawy
- Subjects
Azo dyes ,Biodecolorization ,Biodegradation ,Congo red (CR) ,Kinetics ,Environmental sciences ,GE1-350 - Abstract
Congo red is a synthetic azo-dye dye with many industrial applications. The effluents containing azo dyes are causing several environmental hazards and thus should be treated prior to their discharge. The present work investigates the possible use of a novel microbial consortium from sheep compost for the decolorization of Congo red dye. The effect of different parameters including contact time, dye concentration and inoculum concentration on dye decolorization were investigated. The kinetic of dye decolorization was also assessed and the biodegradation of the dye was confirmed by different techniques. The results showed that the microbial consortium decolorized about 98% of Congo red (500 mg/L) after 24h. The efficiency of the decolorization decreased from 95% to 62% when the dye concentration increased from 100 to 500mg/L. Also, it was noticed that 75% of Congo red (25 mg/L) was decolorized at an inoculum rate of 2.5%. The kinetic results suggested that the decolorization of Congo red by the studied consortium follows the first order kinetic model. Also the maximum substrate consumption rate (Vmax) according to Michaelis- Menten model was found to be 19.30 mg/h/L and the decolorization rate constant (Km) was 116.93 mg/L. The biodegradation of Congo red was further confirmed by HPLC and GC-Ms analysis which revealed the presence of some spectral differences between the untreated dye sample and the treated one. In conclusion, the results of the present work suggest that microbial consortium from sheep compost could have potential application for bioremediation of industrial effluents containing Congo red dye.
- Published
- 2019
- Full Text
- View/download PDF
27. A study on the utility of immobilized cells of indigenous bacteria for biodegradation of reactive azo dyes.
- Author
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Pandey, Koushik, Saha, Purbasha, and Rao, K. V. Bhaskara
- Subjects
- *
AZO dyes , *REACTIVE dyes , *PHYTOTOXICITY , *HIGH performance liquid chromatography , *BIODEGRADATION , *POLLUTION remediation , *IMMOBILIZED cells , *BACILLUS (Bacteria) - Abstract
Azo dyes are recalcitrant compounds used as a colorant in various industries. The pollution caused by their extensive usage has adversely affected the environment for years. The existing physicochemical methods for dye pollution remediation are rather inefficient and hence there is a dearth of low-cost, potential systems capable of dye degradation. The current research studies the biodegradation potential of immobilized bacterial cells against azo dyes Reactive Orange 16 (RO-16) and Reactive Blue 250 (RB-250). Two indigenous dye degrading bacteria Bacillus sp. VITAKB20 and Lysinibacillus sp. KPB6 was isolated from textile sludge sample. Free cells of Bacillus. sp. VITAKB20 degraded 92.38% of RO-16 and that of Lysinibacillus sp. KPB6 degraded 95.36% of RB-250 within 72 h under static conditions. Upon immobilization with calcium alginate, dye degradation occurred rapidly. Bacillus. sp. VITAKB20 degraded 97.5% of RO-16 and Lysinibacillus sp. KPB6 degraded 98.2% of RB-250 within 48 h under shaking conditions. Further, the nature of dye decolorization was biodegradation as evident by high-performance liquid chromatography (HPLC), and Fourier-transform infrared spectroscopy (FTIR) results. Phytotoxicity and biotoxicity assays revealed that the degraded dye products were less toxic in nature than the pure dyes. Thus, immobilization proved to be a highly likely alternative treatment for dye removal. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
28. Biodecolorization of textile azo dye using Bacillus sp. strain CH12 isolated from alkaline lake
- Author
-
Awoke Guadie, Samson Tizazu, Meseretu Melese, Wenshan Guo, Huu Hao Ngo, and Siqing Xia
- Subjects
Alkaline lake ,Bacillus sp. ,Biodecolorization ,Strain CH12 ,Reactive Red 329 ,Biotechnology ,TP248.13-248.65 - Abstract
Textile azo dye decolorizing bacteria were isolated from alkaline Lakes Abaya and Chamo using Reactive Red 239 (RR239) dye. Through subsequent screening process, strain CH12 was selected to investigate the effects of nutrient supplement, DO, pH, temperature, dye concentration and types on decolorization. Based on 16S rRNA gene sequence analysis, strain CH12 was identified as Bacillus sp. Decolorization efficiencies were significantly enhanced with carbon (≥98%) and organic nitrogen (∼100%) supplements. Complete decolorization was also observed under anoxic and anaerobic conditions, and at the temperature of 30 °C and the pH of 10. However, the azo dye decolorization efficiency of strain CH12 was significantly reduced when NaNO3 (1–8%) was supplemented or under aerobic culturing condition (≤6%), indicating that RR239 was less preferred electron acceptor. Overall, strain CH12 can be a promising candidate for decolorization applications due to its potential to effectively decolorize higher RR239 concentrations (50−250 mg/L) and six additional dyes.
- Published
- 2017
- Full Text
- View/download PDF
29. Textile Dye Biodecolorization by Manganese Peroxidase: A Review
- Author
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Yunkang Chang, Dandan Yang, Rui Li, Tao Wang, and Yimin Zhu
- Subjects
manganese peroxidase ,biodecolorization ,dye wastewater ,immobilization ,recombinant enzyme ,Organic chemistry ,QD241-441 - Abstract
Wastewater emissions from textile factories cause serious environmental problems. Manganese peroxidase (MnP) is an oxidoreductase with ligninolytic activity and is a promising biocatalyst for the biodegradation of hazardous environmental contaminants, and especially for dye wastewater decolorization. This article first summarizes the origin, crystal structure, and catalytic cycle of MnP, and then reviews the recent literature on its application to dye wastewater decolorization. In addition, the application of new technologies such as enzyme immobilization and genetic engineering that could improve the stability, durability, adaptability, and operating costs of the enzyme are highlighted. Finally, we discuss and propose future strategies to improve the performance of MnP-assisted dye decolorization in industrial applications.
- Published
- 2021
- Full Text
- View/download PDF
30. Biodecolorization of textile reactive dyes by Penicillium chrysosporium and Penicillium simplicissimum-A comparative study
- Author
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Muthukumaran, P, Paramasivan, T., Priya, M., Abarna, S., Ramalingam, P., and Saraswathy, N.
- Published
- 2017
- Full Text
- View/download PDF
31. /+//Biodecolorization of Reactive Black5 and Reactive Red120 azo dyes using bacterial strains isolated from dairy effluents.
- Author
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Sadeghi, M., Forouzandeh, S., Nourmoradi, H., Heidari, M., Ahmadi, A., Jami, M. S., Abdizadeh, R., and Mohammadi-Moghadam, F.
- Abstract
In a biological treatment of an industrial effluent, the indigenous bacteria have already been used. In this study, the three bacteria strains which are potent in the decolorization of azo dyes were isolated from dairy effluent and used for decolorization of Reactive Black5 and Reactive Red120 azo dyes and decolorization test of each dye was conducted at five concentration levels (10, 50, 100, 150, and 200 mg/l). The pH of which was adjusted to 7 and incubated at 37 °C for 3 days. The strains were identified as Staphylococcus sp. and Micrococcus luteus strains by 16S rRNA gene sequences analysis. The strains were deposited in GenBank with accession numbers of KX180131, KX180132, and KX180133 and submitted to laboratory identifier named Staphylococcus sp. MEH038S, Micrococcus luteus strain SEH038S, and Micrococcus luteus strain FEH038S. Three days into incubation, the lowest efficiency was at a concentration level of 200 mg/l for each dye. Decolorization efficiencies for Staphylococcus sp. MEH038S, Micrococcus luteus strain SEH038S, and Micrococcus luteus strain FEH038S at the concentration level of 200 mg/l for Reactive Red120 were 89.7, 87.1, and 89.3%, and for Reactive Black5 were 90.8, 90.0, and 89.9%, respectively. Based upon this study, dairy effluents can be used as a suitable alternative to the decolorization of textile wastewater. This study demonstrates a report on grounds of elucidation for the use of non-indigenous bacteria in the treatment of industrial wastewater. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
32. Improved Bacterial-Fungal Consortium as an Alternative Approach for Enhanced Decolourisation and Degradation of Azo Dyes: A Review.
- Author
-
Mani, Arunkumar and Shahul Hameed, Sheik Abdulla
- Subjects
AZO dyes ,TEXTILE dyeing ,TEXTILE industry ,BIOREMEDIATION ,MUTAGENESIS - Abstract
Over the past five decades, the Indian textile industries have increasingly become a major user of azo dyes due to their wide range of applications. India is considered as one of the second largest producers of textiles in the world. Due to the rapid increase in the production rate of fabrics, the textile industry generates a huge amount of dyestuff and dyes in the environment. Continuous discharge of wastewater into the environment creates various types of pollution and also health impact on human beings. Azo dyes are toxic aromatic man-made compounds, which cannot be easily degraded by the indigenous microbes, and thus persist in ecosystems over a long period of time. In the current scenario, most wastewater treatment plants are obsolete and especially physico-chemical methods failed to achieve the satisfactory result because they are more expensive and produce a huge amount of sludge which again causes the secondary disposal problems. So, there is an urgent need to develop an alternative method to improve the degradation of azo dyes in an eco-friendly manner with low cost. In order to achieve the higher degradation efficiency in bioremediation technology, the present review mainly focuses on three important factors such as strain development for hyper-production of oxidoreductive enzymes by random mutagenesis, development of bacterial-fungal consortium and reusability of microbial cell cultures. Additionally, this review also compiles the effect of various physico-chemical conditions in decolourisation and degradation of azo dyes. [ABSTRACT FROM AUTHOR]
- Published
- 2019
33. Decolorization of Synthetic Dyes by Ligninolytic Enzymes from Trametes hirsuta D7.
- Author
-
Anita, Sita Heris, Sari, Fahriya Puspita, and Yanto, Dede Heri Yuli
- Subjects
OIL palm ,GENTIAN violet ,ENZYMES ,DYES & dyeing ,WATER purification - Abstract
The ability of ligninolytic enzymes from Trametes hirsuta D7 to decolorize several synthetic dyes was investigated. A crude enzyme powder was produced by fermenting oil palm empty fruit bunch fibers for one month. The dye decolorization by the enzymes occurred at an efficient 0.25 U/mL. The enzymes degraded 100, 200, 300, 400, and 500 ppm Remazol Brilliant Blue R (RBBR) within 7 h by 95.57 ± 0.32%, 93.46 ± 3.09%, 91.84 ± 0.65%, 86.44 ± 0.97%, and 82.14 ± 0.52%, respectively. The enzyme also decolorized anthraquinone (Acid Blue 129), monoazo (Acid Orange 7), diazo (Reactive Black 5), and trimethyl methane (Methyl Violet) dyes within 7 h by 94.59 ± 7.97%, 13.99 ± 0.30%, 7.61 ± 0.01%, and 7.59 ± 0.18%, respectively. Addition of MnSO
4 , H2 O2 , and violuric acid enhanced the dye decolorization rate by up to 10-fold. This study shows the potential for application of ligninolytic enzymes from T. hirsuta D7 in the treatment of wastewater effluent of textile industries. [ABSTRACT FROM AUTHOR]- Published
- 2019
- Full Text
- View/download PDF
34. Biodecolorization of biogas plant effluent derived from anaerobically digested distillery wastewater by naturally selected Pseudomonas putida.
- Author
-
Kumkaew, Ponruedee, Suaisom, Pitchaya, Mukkata, Kanokwan, Koonaphapdeelert, Sirichai, Sawatdeenarunat, Chayanon, and Nitayavardhana, Saoharit
- Subjects
- *
PSEUDOMONAS putida , *RADIATION sterilization , *BIOGAS , *ACTIVATED sludge process , *WATER purification , *SEWAGE disposal plants , *SEWAGE sludge - Abstract
Effluent from biogas plants can contribute to serious environmental issues due to its high organic compound content and its dark color. This study utilizes Pseudomonas putida strains isolated from activated sludge in a wastewater treatment plant to reduce the organic compound content and dark color of biogas plant effluent. The treatment of effluent from the anaerobic digestion of distillery wastewater with P. putida in the best evaluated conditions (sample concentration of 50% v/v , agitation and aeration rates of 250 rpm and 1.0 vvm for 6 days) produced significant decolorization and organic removal efficacies of 34.29 ± 0.87% and 38.94 ± 0.63%, respectively. Bioremediation efficiency was dependent on bacterial growth, and the organic content and dissolved oxygen in the sample during cultivation were key factors in bacterial growth. The naturally selected bacterium could tolerate relatively high levels of organic content and work synergistically with other indigenous microorganisms found in the biogas plant effluent. Therefore, the bioremediation of biogas plant effluent using naturally selected P. putida can feasibly be applied in biogas power plants where sterilization is not necessary. [Display omitted] • Natural selected P. putida effectively decolorized biogas effluent (34% removal) • P. putida can work synergistically with indigenous microbes in biogas effluent • Sterilization is not necessary for large-scale bioremediation process [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
35. Decolorization of Azo Dyes by Local Microorganisms
- Author
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Johari, Wan Lutfi Wan, Isa, Razlin Izwan Mohd, Ghazali, Norhusna, Arif, Noorliza Mat, Shukor, Mohd Yunus Abd, Aris, A.Z., editor, Tengku Ismail, T.H., editor, Harun, R., editor, Abdullah, A.M., editor, and Ishak, M.Y., editor
- Published
- 2014
- Full Text
- View/download PDF
36. BIODECOLORIZATION OF TEXTILE INDUSTRIAL WASTE BY THERMOPHILIC BACTERIA Anoxybacillus rupiensis TS04 AND Anoxybacillus flavithermus TS15
- Author
-
Muharni Muharni, Heni Yohandini, and M Yunus Rivai
- Subjects
Biodecolorization ,textile ,industrial ,waste, thermophilic ,bacteria ,Biology (General) ,QH301-705.5 - Abstract
Wastewater of textile industries contained a high content of synthetic dyes that could damage the aquatic ecosystem and environmental aesthetics. The use of microorganisms on the biodecolorization of textile industrial waste had advantages such as low cost and environmentally friendly. The purpose of the study was to determine biodecolorization capability of Anoxybacillus rupiensis TS04 and Anoxybacillus flavithermus TS15 for industrial waste of tie-dye fabrics. Completely randomized design with factorial pattern was used in the research; factor I and II were concentrations of wastewater and types of bacteria, respectively Anoxybacillus rupiensis TS04 showed the highest decolorization ability by 83.25% for wastewater concentration of 80% (v/v) and Anoxybacillus flavithermus TS15 by 69% at 40% (v/v) waste concentration.The highest cell number of Anoxybacillus rupiensis TS04 was obtained as 1.52 x 106 cfu/mL and biodecolorization, textile industrial waste, thermophilic bacteria,Anoxybacillus flavithermus TS15 3.70 x 105cfu/mL.
- Published
- 2018
- Full Text
- View/download PDF
37. Investigation of dye removal with isolated biomasses from whey wastewater.
- Author
-
Sofu, A.
- Abstract
In this work, as a new eco-friendly biosorbent, the removal of textile wastewater was carried out by biomass of mesophilic and thermophilic lactic acid bacteria (LAB), which form the natural flora of whey. Mesophilic and thermophilic LAB provide a wide variety of properties in the taste and texture of food products through the fermentation of raw materials in the production of dairy products. Whey is produced as waste after dairy products are produced. Decolorization tests of Dorasyn Red azo dye, which is widely used in the textile sector, have been carried out by obtaining biomass with LAB isolated from whey. Thus, the recovery of whey, which is accepted as waste, has been achieved. In this study, parameters affecting the dissociation reaction of different pH and biomass concentrations were investigated. The dye removal test for four different LAB biomasses (mesophilic lactobacilli and lactococci, and thermophilic lactobacilli and lactococci) was performed at ten different concentration levels (0, 0.5, 0.75, 1, 1.25, 1.5, and 5 g/L). The pH value was adjusted to pH 3 and 6.8 (original) and incubated for 3 h at 20 °C. Tested pH values were 1.5, 2, 3, 4, 5, 6, 7, 8, 9, and 10 with constant 2.5 g/L biomass concentration. Decolorization efficiencies for about 90% dye removal were obtained with mesophilic lactobacilli biomass (2.5 g/L) at pH 3 after 3-h reaction time. This study presents a report that the whey for environmental protection and paint recovery is an appropriate alternative to the decolorization of textile wastewater. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
38. A novel microbial consortium from sheep compost for decolorization and degradation of Congo red.
- Author
-
Abd El-Kader, F., El-Chaghaby, G. A., Khalafalla, G. M., Refae, R. I., and Elshishtawy, H. M.
- Subjects
CONGO red (Staining dye) ,CHEMICAL decomposition ,BIODEGRADATION ,INDUSTRIAL applications ,COMPOST & the environment ,SHEEP as laboratory animals - Abstract
Congo red is a synthetic azo-dye dye with many industrial applications. The effluents containing azo dyes are causing several environmental hazards and thus should be treated prior to their discharge. The present work investigates the possible use of a novel microbial consortium from sheep compost for the decolorization of Congo red dye. The effect of different parameters including contact time, dye concentration and inoculum concentration on dye decolorization were investigated. The kinetic of dye decolorization was also assessed and the biodegradation of the dye was confirmed by different techniques. The results showed that the microbial consortium decolorized about 98% of Congo red (500 mg/L) after 24h. The efficiency of the decolorization decreased from 95% to 62% when the dye concentration increased from 100 to 500mg/L. Also, it was noticed that 75% of Congo red (25 mg/L) was decolorized at an inoculum rate of 2.5%. The kinetic results suggested that the decolorization of Congo red by the studied consortium follows the first order kinetic model. Also the maximum substrate consumption rate (V
max ) according to Michaelis- Menten model was found to be 19.30 mg/h/L and the decolorization rate constant (Km ) was 116.93 mg/L. The biodegradation of Congo red was further confirmed by HPLC and GC-Ms analysis which revealed the presence of some spectral differences between the untreated dye sample and the treated one. In conclusion, the results of the present work suggest that microbial consortium from sheep compost could have potential application for bioremediation of industrial effluents containing Congo red dye. [ABSTRACT FROM AUTHOR]- Published
- 2019
- Full Text
- View/download PDF
39. Optimization and mechanisms for biodecoloration of a mixture of dyes by Trichosporon akiyoshidainum HP 2023.
- Author
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Martorell, María M., Rosales Soro, María del M., Pajot, Hipólito F., and de Figueroa, Lucía I. C.
- Subjects
MICROORGANISMS ,DYES & dyeing - Abstract
Trichosporon akiyoshidainum HP2023 is a basidiomycetous yeast isolated from Las Yungas rainforest (Tucumán, Argentina) and selected based on its outstanding textile-dye-decolorizing ability. In this work, the decolorization process was optimized using Reactive Black 5 as dye model. Lactose and urea were chosen as carbon and nitrogen sources through a one-at-time approach. Afterwards, factorial designs were employed for medium optimization, leading to the formulation of a simpler optimized medium which contains in g L
−1 : lactose 10, yeast extract 1, urea 0.5, KH2 PO4 1 and MgSO4 1. Temperature and agitation conditions were also optimized. The optimized medium and incubation conditions for dye removal were extrapolated to other dyes individually and a mixture of them. Dye removal process happened through both biosorption and biodegradation mechanisms, depending primarily on the dye structure. A positive relation between initial inoculum and dye removal rate and a negative relation between initial dye concentration and final dye removal percentages were found. Under optimized conditions, T. akiyoshidainum HP2023 was able to completely remove a mixture of dyes up to a concentration of 300 mg L−1 , a concentration much higher than those expected in real effluents. [ABSTRACT FROM AUTHOR]- Published
- 2018
- Full Text
- View/download PDF
40. New approach for the biodecolorization of Remazol Black-B (RB-B) by Streptomyces hygroscopicus strain PTCC1132.
- Author
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Tahamtan, Tayyebeh, Alvani, Vida, Bhatnagar, Amit, Leili, Mostafa, Gharehchahi, Ehsan, and Ansarizadeh, Mohammad
- Subjects
COLOR removal (Sewage purification) ,STREPTOMYCES hygroscopicus ,AZO dyes - Abstract
The textile industry is a rapidly developing industrial sector that produces – wastewater laden with various pollutants including synthetic dyes and heavy metals. Remazol Black-B (RB-B) is a group of azo dyes that are extensively used in textile industries. The objective of this study was to evaluate the potential of the Streptomyces hygroscopicus PTCC1132 to remove RB-B and to determine optimal conditions for decolorizing. The Taguchi optimization approach was used to reduce the number of experiments and the time needed to find optimum conditions. The effect of operating parameters such as temperature, pH, initial RB-B concentrations, and salt dosage were evaluated by defined factor 4 on four-level Taguchi L16 orthogonal array. Qualitek-4 software was used for data analysis. Maximum efficiency for RB-B decolorization was obtained at 33°C; wherein the solution pH was set to 9.0; and RB-B concentration and salt concentration were 5,000 mg/L and 1%, respectively. The corresponding decolorization efficiency was obtained as 95.27% at under optimized conditions confirming that the bacteria exhibits resistance against the toxic effects of RB-B, especially at high dye concentrations. Our findings indicate of the effectiveness of the evaluated microorganisms for the removal of RB-B from dye-containing effluents. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
41. Anaerobic decolorization and detoxification of cationic red X-GRL by Shewanella oneidensis MR-1.
- Author
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Li, Qian, Feng, Xiao-Li, Li, Ting-Ting, Lu, Xue-Rong, Liu, Qiu-Yue, Han, Xue, Feng, Yu-Jie, Liu, Zhao-Ying, Zhang, Xi-Jia, and Xiao, Xiang
- Subjects
ANAEROBIC metabolism ,DETOXIFICATION (Substance abuse treatment) ,SALINITY & the environment - Abstract
The ability of an electrochemically active bacterium, Shewanella oneidensis MR-1, to decolorize azo dye cationic red X-GRL (X-GRL) was investigated. S. oneidensis MR-1 showed a high decolorization capability for X-GRL under anaerobic conditions. The Mtr respiratory pathway was proved to be involved in the extracellular decolorization of X-GRL. The decolorization efficiency of S. oneidensis MR-1 was significantly inhibited when the initial X-GRL concentration was over 200 mg L
−1 . Increasing the inoculum volume of S. oneidensis MR-1 could obviously promote the X-GRL decolorization. The 100 mg L−1 X-GRL and 6% (v/v) inoculum volume were chosen as the optimal parameter. Under such a condition, almost all of X-GRL (100 mg L−1 ) could be completely reduced after 12-h incubation at the pH range of 5.5-8.0 and temperature range of 30-40°C. Salinity in the medium also affected X-GRL decolorization. Lactate and citric acid were found to be the suitable electron donors for X-GRL decolorization. Although the genotoxicity increased slightly, the phytotoxicity of X-GRL in the decolorization process was significantly reduced by S. oneidensis MR-1. [ABSTRACT FROM AUTHOR]- Published
- 2018
- Full Text
- View/download PDF
42. Exploring the potential of halophilic archaea for the decolorization of azo dyes.
- Author
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Masoomeh Selseleh Hassan Kiadehi, Mohammad Ali Amoozegar, Asad, Sedigheh, and Siroosi, Maryam
- Subjects
- *
HALOPHILIC microorganisms , *AZO dyes , *HALOBACTERIUM , *YEAST extract , *GLUCOSE - Abstract
Azo dyes are being extensively used in textile industries, so finding a proper solution to decolorize them is of high importance. In order to find azo dye decolorizing strains among haloarchaea, which are well known for their tolerance to harsh environmental conditions, fifteen haloarchaeal strains were screened. Halogeometricum sp. strain A and Haloferax sp. strain B with the highest decolorization ability (95% and 91% for Remazol black B; both about 60% for Acid blue 161, respectively) were selected for further studies. It was shown that both strains were able to grow and decolorize the dye in a medium containing up to 5 M NaCl, with optimum decolorization activity at 2.5-3.4 M, pH 7 and a wide temperature range between 30 to 45 °C. Moreover, both strains were able to tolerate and decolorize up to 1,000 mg l-1 Remazol black B. Also, they were able to survive in 5,000 mg l-1 of the dye after 20 days'incubation. Glucose and yeast extract were found to be the best carbon and nitrogen sources in the decolorization medium for both strains. This is the first report studying decolorization of azo dyes using halophilic archaea. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
43. Methylene Blue biodecolorization and biodegradation by immobilized mixed cultures of Trichoderma viride and Ralstonia pickettii into SA-PVA-Bentonite matrix.
- Author
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Nabilah, Badzlin, Purnomo, Adi Setyo, Prasetyoko, Didik, and Rohmah, Alya Awinatul
- Abstract
The problem of industrial dye wastewater poses a critical environmental challenge that demands urgent attention. This is because the direct release of synthetic dyes such as Methylene Blue (MB) into water bodies has been found to have adverse effects on the environment. Therefore, this study aimed to propose immobilization of a mixed Trichoderma viride and Ralstonia pickettii culture into Sodium alginate–Polyvinyl Alcohol-Bentonite (SA-PVA-Bentonite) matrix as a development method for MB decolorization and degradation. Immobilization process was carried out using the entrapment method, where bacteria and fungi cells were homogenized into the SA-PVA-Bentonite matrix. The results showed that immobilized culture (IMO Mix) outperformed the free cells in Mineral Salt Medium (MSM), achieving an impressive 97.88% decolorization rate for 48 h at 30 °C. Furthermore, a total of 3 metabolite product degradation were produced including Azure A and C, as well as Thionine by LCMS analysis. SEM-EDX analysis confirmed that culture was agglomerated within the SA-PVA-Bentonite matrix, while FTIR demonstrated the functional groups of the synthesized beads. Meanwhile, the difference in charge of bentonite facilitated the adsorption of MB onto the beads, and mixed culture supported the degradation process. This study presented a potential solution to environmental problems, particularly those related to the industrial sector. Further analysis was required to address the challenges associated with other industrial dye waste. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
44. Comparative study of eco- and cytotoxicity during biotransformation of anthraquinone dye Alizarin Blue Black B in optimized cultures of microscopic fungi.
- Author
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Rybczyńska-Tkaczyk, Kamila, Święciło, Agata, Szychowski, Konrad A., and Korniłłowicz-Kowalska, Teresa
- Subjects
CELL-mediated cytotoxicity ,BIOTRANSFORMATION in microorganisms ,ANTHRAQUINONE dyes ,ALIZARIN ,MICROFUNGI - Abstract
The aim of this study was to select optimal conditions (C and N sources, initial pH and temperature) for biodecolorization of 0.03% anthraquinone dye Alizarin Blue Black B (ABBB) by microscopic fungi: Haematonectria haematococca BwIII43, K37 and Trichoderma harzianum BsIII33. The phenolic compounds, phytotoxicity ( Lepidium sativum L.), biotoxicity (Microtox), cytotoxicity and yeast viability assay were performed to determine the extent of ABBB detoxification. Biodecolorization and detoxification of 0.03% ABBB in H. haematococca BwIII43 and T. harzianum BsIII33 cultures was correlated with extracellular oxidoreductases activity. In turn, secondary products, toxic to human fibroblasts and respiring sod1 Saccharomyces cerevisiae cells, were formed in H. haematococca K37 strain cultures, despite efficient decolorization. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
45. Bio-decolorization of synthetic dyes by a novel endophytic fungus Penicillium janthinellum LM5 from blueberry pulp.
- Author
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Yan, Jufen, Wang, Pei, Wang, Longxin, Jin, Qi, Ali, Abdallah S., He, Yongsheng, Wang, Ying, Sun, Yuxuan, Li, Aoqiang, Adwy, Waly, Ahmed, Rasha H., and Han, Xinya
- Subjects
- *
ENDOPHYTIC fungi , *PENICILLIUM , *CONGO red (Staining dye) , *BLUEBERRIES , *DYES & dyeing , *WASTEWATER treatment , *COLOR removal in water purification - Abstract
Synthetic dyes from textile, paper, leather, and plastic processing, etc. are the majority of the colored effluents and have a harmful impact on human health and environment. Dye biosorption through endophytic fungi will be a challenge. A new fungus isolated from blueberry pulp was identified morphologically and genetically as Penicillium janthinellum LM5. It was utilized for the biosorption of synthetic dyes by forming mycelium particles with efficient decolorization properties in the dye solution. The results of decolorization experiments exhibited that dye type, pH, temperature and time were the important factors and the best decolorization condition on Congo red was pH 6.0, 30 ℃ and 48 h. Moreover, LM5 can achieve 90% decolorization rate even under the high salt concentrations of 200 mg/mL. Based on spectral analysis, the decolorization of Congo red was mainly attributed to the biosorption of mycelium and its pellet structure. Additionally, the fungus adsorbed Congo red can be as an excellent electrode material with a long-cycle performance and stable capacitance property, being a good way for recycling. In summary, this research employed a novel dye treatment method with simultaneous culture and decolorization through an endophytic fungus LM5, being a promising biomaterial in the application of dye wastewater treatment. [Display omitted] • A new species of Penicillium janthinellum LM5 isolated from blueberry pulp. • Endophytic fungi as a novel source of biosorbent. • The simultaneous process of fungal cultivation and decolorization for dye treatment. • Efficient decolorization of mixed dyes with fungus LM5 treatment. • Fungus LM5 adsorbed with dyes as an electrode material, achieving "Trash to Treasure". [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
46. Development of microbial consortium for the biodegradation and biodecolorization of textile effluents
- Author
-
Rajendra Ramasany, Hasab Abdelbagi Muhammad Ahmed, and Sundaram S. Karthik
- Subjects
Microbial consortium ,Biodegradation ,Biodecolorization ,Textile effluents ,Environmental engineering ,TA170-171 - Abstract
In the current study three bacterial species (Bacillus sp., Pseudomonas sp., and Alcaligenes sp.) and two fungal species (Aspergillus sp., and Penicillium sp.) screened from 265 bacterial isolates and 35 fungal isolates respectively, were used in 23 different combinations for the biotreatment of textile waste water collected from Karur, Tiruppur and Coimbatore districts under aerated conditions. The chemical oxygen demand (COD), total solids (TS) total dissolved solids (TDS) & total suspended solids (TSS), hardness, and color intensity of the textile effluent was found to be very high than the permissible limits before treatment. After treatment one particular combination was capable of reducing the COD of the effluent sample by 75%. About five combinations of microbes efficiently reduced the color of the effluent by more than 50%. Another combination was found to be the most effective in the reduction of TS and TDS by 90% and 69%, respectively. Though there was no drastic change in the pH of the sample, it was not of great concern as the pH of the sample was well within the permissible limits for the discharge of the wastewater in to natural sources after treatment.
- Published
- 2012
- Full Text
- View/download PDF
47. Phyto-bacterial biosorption of basic fuchsine: A self-sustainable approach towards biomitigation of contaminant of emerging concern.
- Author
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Jathanna, Neetha Nirupam, Krishnamurthy, Girish Kumar, Paithankar, Jagdish Gopal, Hegde, Smitha, Goveas, Louella Concepta, Ravindranath, Bilachi S., and Gowdru, Mahendra
- Subjects
ENDOPHYTIC bacteria ,BASIC dyes ,BACTERIAL cell surfaces ,ADSORPTION isotherms ,ADSORPTION kinetics ,SORPTION - Abstract
Basic fuchsine dye, a contaminant of emerging concern (CEC) is known for carcinogenicity and mutagenicity on humans and aquatic animals. Environmental persistence of basic fuchsine due to its structural stability is a concern due to its hideous effects on the eco-system. Among various methods of remediation, biological methods of dye adsorption are considered a potential tool and a self-sustainable approach of cleaning contaminated environments. In the current study, a non-pathogenic phyto-symbiotic bacteria, Enterobacter hormaechei subsp. Xiangfangensis strain KG 2 16S isolated from Kaempferia pulchra L., was investigated for the reduction of basic fuchsine dye. One factor at a time (OFAT) studies revealed the optimal conditions for remediation was 20 mg/L of dye with 4 g/L of sucrose when introduced with 4 % v/v of the bacteria for 72 h. Adsorption kinetics demonstrated the biosorption design followed pseudo-first-order (PFO) kinetics as the R
2 value is 0.93, while the adsorption isotherms obey the Langmuir isotherm (R2 value is 0.975). Analytical techniques like LCMS and FTIR analysis of bacterial biomass revealed the dye adsorption onto the bacterial surface. SEM-EDS analysis indicated the presence of Sodium (Na) and Chlorine (Cl) on the bacterial surface. Florescent microscopy of bacteria indicated duration-based dye accumulation and dye internalization and hence the current study is the first to report this phenomenon of biomitigation. In-silico toxicity assay predicted the dye as a probable causative agent of developmental toxicity. Cytogenotoxicity assay indicated reduced toxicity in comparison to the untreated sample. Through this study, we propose phyto-bacterial biosorption as a self-sustainable, eco-friendly approach to reduce the hideous effects on plants, humans, and the environment caused by the bioaccumulation of these carcinogenic dyes. [Display omitted] • Phyto-symbiotic bacteria of K. pulchra L., was assessed for mitigation of 20 mg/L of basic fuchsine. • Biosorption and dye internalization serially witnessed by endophytic bacteria at 72 h of treatment. • Reduced cytogenotoxicity witnessed post phyto-bacterial dye mitigation with reduced aberrant index of 7%. • Eco-friendly and cost-effective biosorption using viable bacteria was proposed. • Viable cells as biosorbents anticipated sustainable over existing methodologies. [ABSTRACT FROM AUTHOR]- Published
- 2023
- Full Text
- View/download PDF
48. REMOCIÓN DE COLORANTES POR MEDIO DE CURCUBITACEAS.
- Author
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Barajas, Juanita, Cano, Maribel, Castorena, José H., Santiago, Victor, and Díaz, Joel
- Published
- 2016
49. Mutual interactions of Pleurotus ostreatus with bacteria of activated sludge in solid-bed bioreactors.
- Author
-
Svobodová, Kateřina, Petráčková, Denisa, Kozická, Barbora, Halada, Petr, and Novotný, Čeněk
- Subjects
- *
PLEUROTUS ostreatus , *ACTIVATED sludge process , *BIOREACTOR research , *XENOBIOTICS , *SEWAGE - Abstract
White rot fungi are well known for their ability to degrade xenobiotics in pure cultures but few studies focus on their performance under bacterial stress in real wastewaters. This study investigated mutual interactions in co-cultures of Pleurotus ostreatus and activated sludge microbes in batch reactors and different culture media. Under the bacterial stress an increase in the dye decolorization efficiency (95 vs. 77.1 %) and a 2-fold elevated laccase activity (156.7 vs. 78.4 Ul) were observed in fungal-bacterial cultures compared to pure P. ostreatus despite a limited growth of bacteria in mixed cultures. According to 16S-rDNA analyses, P. ostreatus was able to alter the structure of bacterial communities. In malt extract-glucose medium the fungus inhibited growth of planktonic bacteria and prevented shifts in bacterial utilization of potential C-sources. A model bacterium, Rhodococcus erythropolis responded to fungal metabolites by down regulation of uridylate kinase and acetyl-CoA synthetase. Graphical Abstract: [Figure not available: see fulltext.] [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
50. Use of RSM modeling for optimizing decolorization of simulated textile wastewater by Pseudomonas aeruginosa strain ZM130 capable of simultaneous removal of reactive dyes and hexavalent chromium.
- Author
-
Maqbool, Zahid, Hussain, Sabir, Ahmad, Tanvir, Nadeem, Habibullah, Imran, Muhammad, Khalid, Azeem, Abid, Muhammad, and Martin-Laurent, Fabrice
- Subjects
WASTEWATER treatment ,COLOR removal (Sewage purification) ,REACTIVE dyes ,HEXAVALENT chromium & the environment ,METAL ions ,ISOLATION of biotechnological microorganisms - Abstract
Remediation of colored wastewater loaded with dyes and metal ions is a matter of interest nowadays. In this study, 220 bacteria isolated from textile wastewater were tested for their potential to decolorize each of the four reactive dyes (reactive red-120, reactive black-5, reactive yellow-2, and reactive orange-16) in the presence of a mixture of four different heavy metals (Cr, Zn, Pb, Cd) commonly found in textile effluents. Among the tested bacteria, the isolate ZM130 was found to be the most efficient in decolorizing reactive dyes in the presence of the mixture of heavy metals and was identified as Pseudomonas aeruginosa strain ZM130 by 16S rRNA gene analysis. The strain ZM130 was highly effective in simultaneously removing hexavalent chromium (25 mg L) and the azo dyes (100 mg L) from the simulated wastewater even in the presence of other three heavy metals (Zn, Pb, Cd). Simultaneous removal of chromium and azo dyes ranged as 76.6-98.7 % and 51.9-91.1 %, respectively, after 180 h incubation. On the basis of quadratic polynomial equation and response surfaces given by the response surface methodology (RSM), optimal salt content, pH, carbon co-substrate content, and level of multi-metal mixtures for decolorization of reactive red-120 in a simulated textile wastewater by the strain ZM130 were predicted to be 19.8, 7.8, and 6.33 g L and a multi-metal mixture (Cr 13.10 mg L, Pb 26.21 mg L, Cd 13.10 mg L, Zn 26.21 mg L), respectively. Moreover, the strain ZM130 also exhibited laccase and nicotinamide adenine dinucleotide (reduced)-dichlorophenolindophenol reductase (NADH-DCIP reductase) activity during the decolorization of reactive red-120. However, the laccase activity was found to be maximum in the presence of 300 mg L of the dye as compared to other concentrations. Hence, the isolation of this strain might serve as a potential bio-resource required for developing the strategies aiming at bioremediation of the wastewater contaminated with dyes and heavy metals. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
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