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A novel water-soluble root canal filling material based on sodium iodide (NaI) has been developed to overcome the limitations of existing iodine-based formulations. However, the biological stability of this approach in animal studies remains unverified. This study evaluated the biocompatibility of NaI compared to commercial root canal filling materials (Calcipex II and Vitapex®) in pulpectomized canine teeth to assess its clinical applicability. Following a four-week observation period, none of the experimental groups exhibited tooth mobility or fistula formation. Radiographic and micro-CT analyses revealed no radiolucency in periapical lesions. Histopathologic evaluation demonstrated the absence of inflammatory responses in periapical regions across all material groups, with histological inflammation scoring 0. High-magnification histological examination of periapical areas showed well-preserved periodontal ligament tissue in all groups. Despite certain limitations of NaI-based fillings in the pulp cavity, including loss of radiopacity and tooth discoloration, NaI demonstrates potential as a safe and effective alternative for pulp filling material, particularly due to its minimal risk of root resorption and inflammatory response. [ABSTRACT FROM AUTHOR]

Pressure ulcers are a significant health problem that affects a large population, especially the elderly and individuals with physical limitations. These injuries cause pain, are difficult to heal, and can be expensive to manage, leading to a negative impact on the quality of life of those affected. This scientific paper provides an overview of medical devices such as support surfaces, dressings, and topical agents for preventing and managing pressure ulcers. This review focuses on the importance of understanding the viscoelastic mechanical properties, water vapor transmission rate, and biocompatibility testing of medical devices, which can help define performance criteria needed to prevent and manage pressure ulcers effectively. The paper highlights the potential use of alginate, polyurethane, silicone, polyvinyl alcohol, and collagen as pressure relief and wound care solutions. Synthesizing this research can help medical device manufacturers make better decisions and improve the quality of care for patients with pressure ulcers. [ABSTRACT FROM AUTHOR]

Several hydrogels could be used as scaffolds for tissue engineering and a model of extracellular matrices for biological studies. However, the scope of alginate in medical applications is often severely limited by its mechanical behavior. In the present study, the modification of the mechanical properties of the alginate scaffold is obtained by its combination with polyacrylamide in order to obtain a multifunctional biomaterial. The advantage of this double polymer network is due to an improvement in the mechanical strength with regard to the alginate alone, and in particular, its Young's modulus values. The morphological study of this network was carried out by scanning electron microscopy (SEM). The swelling properties were also studied over several time intervals. In addition to mechanical property requirements, these polymers must meet several biosafety parameters as part of an overall risk management strategy. Our preliminary study illustrates that the mechanical property of this synthetic scaffold depends on the ratio of the two polymers (alginate, polyacrylamide) which allows us to choose the appropriate ratio to mimic replaceable body tissue and be used in various biological and medical uses, including 3D cell culture, tissue engineering, and protection against local shocks. [ABSTRACT FROM AUTHOR]

This study aimed to detect the biocompatibility of bioactivated polycaprolactone/silk fibroin-based nanofibers in vivo using zebrafish embryos. Anti-Bisphenol A (BPA) antibody or lactase enzyme was immobilized on electrospun nanofibers, for making the nanofiber bioactive. Lactase immobilized nanofiber was developed to hydrolyze lactose and produce milk with reduced lactose. Anti-BPA antibody immobilized nanofiber was developed to remove bisphenol A from liquids. To test the biocompatibility of the bioactive nanofibers, the zebrafish embryos were divided into 4 groups; control, raw nanofiber, lactase immobilized nanofiber, and anti-BPAantibody immobilized nanofiber groups. In nanofiber-based exposure groups; nanofibers were incubated separately in the embryonic development medium. Subsequently, the embryos were kept in these development mediums for 72 h post-fertilization (72 hpf) and their developmental analyzes were performed. At the end of 72 hpf, zebrafish embryos were homogenized. Lipid peroxidation and nitrite oxide levels, and superoxide dismutase and glutathione-S-transferase activities were determined to monitor the disturbance of oxidant-antioxidant balance in zebrafish embryos. Exposure to bioactive nanofibers slightly disrupted the oxidant-antioxidant balance, but this change did not affect the mortality and hatching times of the embryos. In conclusion, zebrafish embryos have been effectively used in biocompatibility testing for bioactive nanofibers suggesting that these materials are biocompatible. • This study tested the biocompatibility of bioactivated nanofibers using zebrafish embryos. • PCL/SF based nanofibers were bioactivated with lactase enzyme and anti-BPA antibody. • Exposure slightly disrupted the oxidant-antioxidant balance without affecting embryo mortality. • Bioactive nanofibers were found to be biocompatible. [ABSTRACT FROM AUTHOR]

Aim: To evaluate the biological and mechanical properties of an adhesive with nanostructured silver vanadate (AgVO 3 ). Materials & methods: Specimens in poly(methyl methacrylate) (PMMA) were treated with Ultra Corega Cream (UCCA) denture adhesive with or without AgVO 3 . Biofilms of Candida albicans , Candida glabrata and Streptococcus mutans were grown and the viable cells counted. Fluorescence microscopy was used. The viability of the VERO cell and adhesive strength were evaluated. Results: All concentrations of AgVO 3 reduced the biofilm formation and showed no cytotoxic effect. At 5 min and 24 h, UCCA with 5 and 10% AgVO 3 showed better performance, respectively. Conclusion: AgVO 3 promoted the antibiofilm activity of the adhesive, with a positive effect on the adhesive strength, and was biocompatible.

ABSTRACT The focus of this study was to test the hypothesis that there would be no difference between the biocompatibility of resin-modified glass ionomer cements. Sixty male Wistar rats were selected and divided into four groups: Control Group; Crosslink Group; RMO Group and Transbond Group. The materials were inserted into rat subcutaneous tissue. After time intervals of 7, 15 and 30 days morphological analyses were performed. The histological parameters assessed were: inflammatory infiltrate intensity; reaction of multinucleated giant cells; edema; necrosis; granulation reaction; young fibroblasts and collagenization. The results obtained were statistically analyzed by the Kruskal-Wallis and Dunn test (P

The aim of this double-blind randomized study was to evaluate the biocompatibility of resin-modified glass ionomer cements (RMGIC) by means of morphological and immunohistochemical analyses. RMGICs were selected and divided into four groups: Group CK (Crosslink Orthodontic Band Cement); Group RS (Resilience Light Cure Band Cement) Group RMO (RMO Band Cement), Group TP (Transbond Plus Light Cure Band), and Group C (Control-polyethylene). The materials were implanted in rat subcutaneous tissues, randomly selected for this study. After time intervals of 7, 15, and 30 days the tissues were submitted to morphological analysis. In immunohistochemical analysis, the immuno-marking of antibody CD68 was evaluated. The results obtained were statistically analyzed by the Kruskal-Wallis and Dunn tests ( p < .05). In the morphological analysis after 7 days, Groups RS, RMO and TP showed more intense inflammatory infiltrate ( p = .004) and only Group RMO presented greater intensity of multinucleated giant cells ( p = .027). In the immunohistochemical analysis, Groups RMO and RS were observed to present a larger quantity of CD68+ ( p = .004) in the time interval of 7 days and only Group RMO presented statistically significant difference for this parameter after 15 days ( p = .026). In the time interval of 30 days, Group RMO presented the largest quantity of multinucleated giant cells ( p < .004). The RMGICS Crosslink and Transbond Plus provided significantly better tissue biocompatibility than the Resilience and RMO Cements. [ABSTRACT FROM AUTHOR]

Human endothelial cells (ECs) were sent to the International Space Station (ISS) to determine the impact of microgravity on the formation of three-dimensional structures. For this project, an automatic experiment unit (EU) was designed allowing cell culture in space. In order to enable a safe cell culture, cell nourishment and fixation after a pre-programmed timeframe, the materials used for construction of the EUs were tested in regard to their biocompatibility. These tests revealed a high biocompatibility for all parts of the EUs, which were in contact with the cells or the medium used. Most importantly, we found polyether ether ketones for surrounding the incubation chamber, which kept cellular viability above 80% and allowed the cells to adhere as long as they were exposed to normal gravity. After assembling the EU the ECs were cultured therein, where they showed good cell viability at least for 14 days. In addition, the functionality of the automatic medium exchange, and fixation procedures were confirmed. Two days before launch, the ECs were cultured in the EUs, which were afterwards mounted on the SpaceX CRS-8 rocket. 5 and 12 days after launch the cells were fixed. Subsequent analyses revealed a scaffold-free formation of spheroids in space. [ABSTRACT FROM AUTHOR]

The objective of this study is to evaluate the biocompatibility of composite powder consisting of silica and titania (SiO2 –TiO2) for biomedical applications. The advancement of nanoscience and nanotechnology encourages researchers to actively participate in reinvention of existing materials with improved physical, chemical and biological properties. Hence, a composite/hybrid material has given birth of new materials with intriguing properties. In the present investigation, SiO2 –TiO2 composite powder was synthesised by sol‐gel method and the prepared nanocomposite was characterised for its phase purity, functional groups, surface topography by powder X‐ray diffraction (XRD), Fourier transform infrared spectroscopy (FT‐IR) and scanning electron microscopy. Furthermore, to understand the adverse effects of composite, biocompatibility test was analysed by cell culture method using MG63 osteoblast cell lines as a basic screening method. From the results, it was observed that typical Si–O–Ti peaks in FT‐IR confirms the formation of composite and the crystallinity of the composite powder was analysed by XRD analysis. Further in vitro biocompatibility and acridine orange results have indicated better biocompatibility at different concentrations on osteoblast cell lines. On the basis of these observations, we envision that the prepared silica–titania nanocomposite is an intriguing biomaterial for better biomedical applications. [ABSTRACT FROM AUTHOR]

OBJECTIVE: This study evaluated the response of periapical tissues to the endodontic sealer Endométhasone in root canal fillings short of or beyond the apical foramen. MATERIAL AND METHODS: Twenty root canals of premolars and incisors of 2 mongrel dogs were used. After coronal access and pulp extirpation, the canals were instrumented up to a size 55 K-file and the apical cemental barrier was penetrated with a size 15 K-file to obtain a main apical foramen, which was widened to a size 25 K-file. The canals were irrigated with saline at each change of file. The root canals were obturated either short of or beyond the apical foramen by the lateral condensation of gutta-percha and Endométhasone, originating 2 experimental groups: G1: Endométhasone/short of the apical foramen; G2: Endométhasone/beyond the apical foramen. The animals were killed by anesthetic overdose 90 days after endodontic treatment. The individual roots were obtained and serial histological sections were prepared for histomorphological analysis (H&E and Brown & Brenn techniques) under light microscopy. The following parameters were examined: closure of the apical foramen of the main root canal and apical opening of accessory canals, apical cementum resorption, intensity of the inflammatory infiltrate, presence of giant cells and thickness and organization of the apical periodontal ligament. Each parameter was scored 1 to 4, 1 being the best result and 4 the worst. Data were analyzed statistically by the Wilcoxon nonparametric tests (p=0.05). RESULTS: Comparing the 2 groups, the best result (p