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Introduction: Africa faces immense food and health insecurity challenges, a problem partly attributed to food loss and waste during postharvest handling and distribution. In the context of research to meet the sustainable development goals, this project specifically addressed the postharvest loss of the ripe indigenous eggplant (Solanum anguivi lam) fruit called Igba Yinrin by Yoruba in South-West Nigeria, which is usually discarded in farms. The study was carried out on ripe and unripe fruits to better understand their value by comparing their effects in diabetes treatment. Methods: The study sought to assess the effects of a diet including ripe or unripe mature eggplant fruits in the sucrose-induced diabetic-like fruit fly. Bioactive compounds were identified and quantified with HPLC-UV, while the antioxidant vitamin (A, C, E), carotenoid, and mineral (Na, K, Ca, Mg, Fe, P, and Zn) content was analyzed in the fruits. Extracts were used to investigate their in vitro anti-inflammatory properties on cyclooxygenases (COX 1 and 2), 5-lipoxygenase (5-LOX), and anti-diabetes enzymes [α-amylase and α-glucosidase], while extract-supplemented diets (0.25-1% concentration) were fed to the fruit flies for 14 days. Results: Interestingly, the results showed that the ripe fruits had a significantly (p < 0.05) higher total phenol and flavonoid content, as well as a higher content of vitamins, carotenoids, and minerals, than the unripe fruits. The in vivo activities of antioxidant enzymes [superoxide dismutase (SOD), catalase (CAT), and glutathione transferase (GST)] and the total thiol level increased, while the blood glucose, reactive oxygen species (ROS), and malondialdehyde (MDA) levels decreased in Drosophila melanogaster (fruit fly). An in silico docking analysis showed strong binding affinity of the above-mentioned enzymes under investigation with the ligands hesperidin, naringin, and myricetin, which are bioactive compounds contained in the examined extracts. Conclusions: There was no significant difference in the biological effects of the ripe and unripe fruit extracts on inflammatory and anti-diabetes enzyme activities, which means that the ripe fruit, usually discarded, could serve as a sustainable alternative source of food nutrients.

The in vitro cultures of Vachellia farnesiana (L) Wight & Arn. have demonstrated cytotoxic activity through callus extract on the HeLa cell line. Explants excised from in vitro-grown seedlings from seeds of two different locations were inoculated on Murashige and Skoog (MS) culture media containing various concentrations of N-6 benzyladenine (BA) or kinetin with 2,4-dichlorophenoxyacetic acid (2,4-D). Optimal efficiency in friable callus induction (100%) was achieved in leaf explants cultured on MS media containing 2.32 µM BA + 13.57 µM 2,4-D. Plant tissues (callus and leaf) were extracted and subjected to quantitative phytochemical analysis, revealing the highest total alkaloid and phenolic content in leaf extracts from Queretaro adult specimens (339.5 ± 20.9 mg atropine equivalents (AE) per g dry extract (DE) and 158.4 ± 12.5 mg gallic acid equivalents (GAE) per g DE, respectively). In contrast, callus cultures exhibited significantly higher total triterpene content (356–381 mg ursolic acid equivalents (UAE) per g DE) compared to leaf extracts (208–243 mg UAE/g DE). Both leaf and callus extracts displayed cytotoxic activity against the HeLa cell line, with a significantly lower half-maximal inhibitory concentration (IC50) for leaf extracts (28–32 µg/mL) compared to callus cultures (43–66 µg/mL), suggesting that alkaloids were primarily responsible for the cytotoxic activity. Furthermore, this study provides valuable insights into the controlled production of bioactive compounds with cytotoxic activity, with callus serving as a rich source. [ABSTRACT FROM AUTHOR]

Practical Application Red pitaya fruit has become a source of natural colorant, because it is rich in betalains, a pigment that imparts a red‐purple color that interests the food and cosmetics industries. This fruit also possesses high nutritional value, with a range of bioactive compounds known to confer potential health benefits and prevent chronic diseases, such as diabetes, which makes it useful for use as pharmaceutical agents and dietary supplements. In order to improve its technological and biological effects, a concentration will be required. Thus, the microfiltration, followed by vacuum concentration, can be an interesting strategy for this purpose. This study aimed to explore tangential microfiltration to produce microfiltered material, which is an important step to obtain the microfiltered red‐purple pitaya concentrate. Therefore, physicochemical and chemical characterization (including 1H NMR analysis) and biological properties (toxicity and diabetes) of this concentrate were assessed, using adult zebrafish as a model. The results show that microfiltration was carried out efficiently, with an average consumption of 95.75 ± 3.13 and 74.12 ± 3.58 kW h m−3, varying according to the material used (“unpeeled pitaya pulp” or “pitaya pulp with peel,” respectively). The in vivo tests indicated non‐toxicity and hypoglycemic effect of the concentrate, since the blood glucose levels were significantly lower in the zebrafish groups treated with this concentrate in comparison with that of control group. Thus, this study suggests the potential of microfiltered red‐purple pitaya concentrate as a promising multifunctional food‐derived colorant, exhibiting beneficial biological effects far beyond its attractive color.Hylocereus polyrhizus (F.A.C. Weber) Britton & Rose has attracted attention as a potential source of natural colorants because of its red‐purple skin and flesh color. In addition, this fruit has a range of bioactive compounds, which make it a valuable resource for providing potential health benefits and preventing chronic diseases such as diabetes. In this paper, the microfiltered red‐purple pitaya concentrate showed beneficial biological effects far beyond its attractive color. Thus, this product can be considered a promising multifunctional food‐derived colorant to use in the food, pharmaceutical, or cosmetics industries. [ABSTRACT FROM AUTHOR]

Background Results Conclusions Pitaya fruit (Hylocereus spp.) is rich in bioactive compounds such as betanin. This study aimed to extract betanin‐rich pitaya fruit and encapsulate it in electrospun nanofibers produced with sweet potato starch. The influence of different concentrations of this bioactive compound on the morphology, functional groups, hydrophilicity, load capacity, color, thermal properties, and contact angle of the electrospun nanofibers with water and milk was assessed. The potential antioxidant and stability of nanofibers during gastrointestinal digestion in vitro were demonstrated.The nanofibers presented average diameters ranging from 134 to 204 nm and displayed homogeneous morphology. The load capacity of the extract in the nanofibers was 43% to 83%. The encapsulation increased the thermal resistance of betanins (197–297 °C). The static contact angle with water and milk showed that these materials presented greater affinity with milk. Principal component analysis (PCA) and hierarchical cluster analysis (HCA) showed that the nanofibers with 5%, 25%, and 45% pitaya extract presented unique characteristics. They showed resistance in delivering betanins to the stomach, with 12% inhibition of the 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH˙) radical. However, only the 45% concentration reached the intestine with 9.83% inhibition of the DPPH˙ radical.Pattern recognition from multivariate analyses indicated that nanofibers containing 5%, 25%, and 45% of the extract presented distinct characteristics, with the ability to preserve betanins against thermal degradation and perform the controlled delivery of these bioactives in the stomach and intestine to produce antioxidant activity. © 2024 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

This study evaluates the antioxidant activity and total phenolic, flavonoid, flavonol, and tannin contents of extracts obtained from the flower parts of Salvia sclarea using different extraction methods, such as maceration and rotavapor (RE). The results indicate that the maceration method yields extracts with higher antioxidant activity compared to the rotavapor method (DPPH IC50: 260.70 ± 65.41 mg/mL vs. 345.48 ± 27.91 mg/mL). Additionally, the maceration method produces higher values in terms of total phenolic (26.4 ± 7.78 mg GAE/g extract), flavonoid (10.44 ± 0.21 mg QE/g extract), flavonol (9.20 ± 0.84 mg QE/g extract), and tannin contents (8.36 ± 0.39 mg GAE/g extract), whereas the RE method shows lower values (phenolics: 19.8 ± 3.31 mg GAE/g extract, flavonoids: 10.35 ± 0.35 mg QE/g extract, flavanol's: 5.45 ± 0.01 mg QE/g extract, and tannins: 7.72 ± 0.10 mg GAE/g extract). The gas chromatography–mass spectrometry (GC–MS) analysis of the extracts identified various bioactive compounds. Molecular docking studies revealed that Terpinen‐4‐ol obtained through maceration exhibits a strong binding affinity to a specific protein target, indicating potential biological activity. The predicted pharmacokinetic (ADME (absorption, distribution, metabolism, and excretion)) parameters for Terpinen‐4‐ol suggest favorable absorption and central nervous system (CNS) penetration, while Squalene obtained through maceration shows different pharmacokinetic properties. S. sclarea highlights significant antioxidant potential and provides insight into the bioactive compounds obtained through different extraction methods. To further investigate the potential biological interactions arising from these differences, molecular docking studies were conducted. [ABSTRACT FROM AUTHOR]

Five Australian seaweed species, Phyllosphora comosa, Ecklonia radiata, Durvillaea potatorum, Sargassum fallax, and Cystophora siliquosa, thrive along the country's shorelines. Some of these seaweeds have recognized health benefits but have not been fully investigated in terms of their bioactive components and mechanisms of action. We employed ultrasonication with 70% methanol to extract phenolic compounds from these seaweeds and investigated a range of bioactivities for these extracts, including anti‐inflammatory activity exploring urease inhibition, nitric oxide scavenging activity, protein denaturation inhibition, and protease inhibition. Anti‐diabetic activities were investigated using α‐amylase and α‐glucosidase inhibition assays. Anti‐proliferative and anti‐mitotic activities were evaluated using yeast‐cell and green‐gram models, respectively. Our findings showed that C. siliquosa inhibited nitric oxide, urease, and protease activities, with S. fallax, P. comosa, and E. radiata exhibiting substantial inhibition of protein denaturation. E. radiata displayed inhibitory effects on both α‐amylase and α‐glucosidase, whereas P. comosa targeted only the α‐glucosidase enzyme, indicating different mechanisms of anti‐diabetic activity. In these anti‐mitotic assays, C. siliquosa exhibited low cell viability and a significant anti‐proliferative effect, particularly within 24 h, while E. radiata demonstrated notable inhibition at 48 h. LC‐ESI‐QTOF‐MS/MS investigation identified 48 phenolic compounds, including 19 phenolic acids, 20 flavonoids, and 9 other polyphenols. The presence of these compounds in extracts correlated with observed biological activities. These results support the potential health benefits of these seaweeds and link this activity to the presence of bioactive phenolics. [ABSTRACT FROM AUTHOR]

Understanding ionic interactions is essential to promote adequate crop nutrition. This study aimed to evaluate the interaction between nitrogen (N) and sulfur (S) and their effects on strawberries' production and postharvest quality. The experiment was conducted under greenhouse conditions adopting a completely randomized design in a 3 × 5 factorial scheme with four replicates. The treatments consisted of the combination of three rates of S (0, 30, and 60 mg dm−3) in the form of calcium sulfate (CaSO4·2 H2O) and five rates of N (0, 30, 60, 90, and 120 mg dm−3) in the form of urea (pro analysis grade) enriched to 10 atom% 15N. The fruits were harvested weekly when showing ¾ maturation. At the end of the harvest, evaluations of the mineral composition of the plants' shoots, determination of N utilization, and physicochemical analyses of the fruit pulp were performed. The results showed that the interaction between N and S affected the fertilizer use efficiency (FUE), strawberry production, and the physicochemical attributes of the fruit pulp. Applying 120 mg dm−3 N and 30 mg dm−3 S improved both strawberry production and postharvest quality. Therefore, balancing N and S levels can improve both the yield and quality of strawberries. [ABSTRACT FROM AUTHOR]

BACKGROUND RESULTS CONCLUSION Pseudostellaria heterophylla, known for its significant bioactive ingredients, offers potential health benefits. Amounts of bioactive compounds of the tuberous root of cultivated Pseudostellaria heterophylla are sensitive to environmental conditions. We selected 22 sampling sites in Guizhou Province, China, a primary Pseudostellaria heterophylla planting area. We analyzed polysaccharides, water‐soluble extractives, total ash and inorganic elements (Fe, Mn, Zn, Mg and Ca) in Radix Pseudostellariae, and pH, organic carbon (OC), available nitrogen (AN), available phosphorus (AP), available potassium (AK) and inorganic elements in the soil.Our study revealed a substantial presence of polysaccharides (85.00–181.00 mg g−1), water‐soluble extractives (47.52–57.63%) and total ash (1.87–3.39%) in Radix Pseudostellariae. Polysaccharides and total ash showed no sensitivity to soil pH. Radix Pseudostellariae collected from soil with pH > 7 exhibited slightly higher levels of water‐soluble extractives, Mg and Ca than that from soil with pH < 5. Conversely, soil with a pH less than 5 had higher OC, AN, AP and AK contents. Water‐soluble extractives in Radix Pseudostellariae were negatively correlated with soil pH but positively correlated with OC and AN.The results imply that the sequestration of soil nutrients over long‐term Pseudostellaria heterophylla cultivation could negatively impact the accumulation of some bioactive ingredients in Radix Pseudostellariae. This study has a profound implication for enhancing the quality of Radix Pseudostellariae of artificially cultivated Pseudostellaria heterophylla. © 2024 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

Summary This study examined the impact of CO2 on the quality of onions stored at 23 °C for 6 weeks, focussing on bioactive compounds, antioxidant capacity, individual anthocyanins, flavonoids and anthocyanin biosynthesis during the storage period. The findings revealed that a 20% CO2 treatment was markedly more effective than the control in maintaining higher levels of phenolics, flavonoids, flavanols and anthocyanins. Moreover, the 20% CO2 treatment preserved significantly higher quantities of individual anthocyanins and flavonoids compared to the control. Throughout storage, the 20% CO2 treatment significantly boosted the activity of enzymes related to anthocyanin biosynthesis and sucrose metabolism relative to the control groups. In summary, the innovation of this study lies in the use of CO2 to maximise anthocyanin and flavonoid content in stored onions, which are usually diminished by extended storage at room temperature. This method could help extend the shelf life of onions while preserving their quality attributes. [ABSTRACT FROM AUTHOR]

BACKGROUND RESULTS CONCLUSION Dictyophora indusiata polysaccharide is an important bioactive component of D. indusiata, playing an important role in alleviating inflammation. The present study aimed to investigate the anti‐inflammatory effect and mechanism of D. indusiata polysaccharide on lipopolysaccharide (LPS)‐induced intestinal inflammation in mice.Our results indicated that D. indusiata polysaccharide ameliorated intestinal inflammation of mice by increasing the body weight, the number of goblet cells and decreasing inflammatory cell infiltration. In addition, D. indusiata polysaccharide significantly up‐regulated expression of ZO‐1, Occuldin mRNA, which were 2.55‐fold and 2.28‐fold higher than the LPS group, respectively. In particular, D. indusiata polysaccharide effectively inhibited the Toll‐like receptor 4 (TLR4)/ c‐Jun NH2‐terminal kinase (JNK) signalling pathway which was 0.34‐fold and 0.49‐fold of gene expression and 0.41‐fold and 0.39‐fold of protein expression in the LPS group, respectively.The results of the present study suggested that D. indusiata polysaccharide exerted anti‐inflammatory and intestinal protective effects by inhibiting the TLR4/JNK signaling pathway, which will provide a basis for the potential value of D. indusiata polysaccharide as prebiotics in food applications. © 2024 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

Aging is associated with a decline in oral immune function, marked by reduced levels of antimicrobial peptides such as defensins. Capsaicin, a bioactive component found in chili peppers, has been theorized to modulate immune responses through specific receptor pathways. This study examined the effects of aging on oral defensin levels and the potential mitigating role of capsaicin, mediated by the immune response in oral tissues. We conducted a comparative analysis between young and aged mice, with or without capsaicin supplementation, for 3 months. The effect of capsaicin was also studied in vitro in senescence‐induced human oral keratinocytes. We found that aging did not reduce defensin levels uniformly but did so in some instances. Capsaicin treatment increased defensin levels in these cases, potentially through transient receptor potential cation channel subfamily V member 1 (TRPV1)‐mediated pathways in the oral cavity. Capsaicin supplementation may counteract age‐related declines in oral defensin levels, enabling the maintenance of oral immune function during aging. [ABSTRACT FROM AUTHOR]

In the face of escalating antibiotic resistance, the quest for novel antimicrobial compounds is critical. Actinobacteria is known for producing a substantial fraction of bioactive molecules from microorganisms, nonetheless there is the challenge of metabolic redundancy in bioprospecting. New sources of natural products are needed to overcome these current challenges. Our present work proposes an unexplored potential of Neotropical social wasp-associated microbes as reservoirs of novel bioactive compounds. Using social wasp-associated Tsukamurella sp. strains 8F and 8J, we aimed to determine their biosynthetic potential for producing novel antibiotics and evaluated phylogenetic and genomic traits related to environmental and ecological factors that might be associated with promising bioactivity and evolutionary specialization. These strains were isolated from the cuticle of social wasps and subjected to comprehensive genome sequencing. Our genome mining efforts, employing antiSMASH and ARTS, highlight the presence of BGCs with minimal similarity to known compounds, suggesting the novelty of the molecules they may produce. Previous, bioactivity assays of these strains against bacterial species which harbor known human pathogens, revealed inhibitory potential. Further, our study focuses into the phylogenetic and functional landscape of the Tsukamurella genus, employing a throughout phylogenetic analysis that situates strains 8F and 8J within a distinct evolutionary pathway, matching with the environmental and ecological context of the strains reported for this genus. Our findings emphasize the importance of bioprospecting in uncharted biological territories, such as insect-associated microbes as reservoirs of novel bioactive compounds. As such, we posit that Tsukamurella sp. strains 8F and 8J represent promising candidates for the development of new antimicrobials. [ABSTRACT FROM AUTHOR]

Background: Traditional medicine claims that Anodendron parviflorum has benefits for treating various human diseases. The present study seeks to understand better the phytochemical and LC–MS/MS-QTOF profiling of A. parviflorum's ethanolic extract and to investigate the properties of the different solvents of A. parviflorum for anti-inflammatory, antioxidant, antimicrobial, toxicity, and cytotoxic effects. Results: The quantitative methods exhibited higher total phenolics (327.16 ± 2.4 mg GAE/g dw), total flavonoid (109.82 ± 1.9 mg QE/g dw), and total alkaloid (14.13 ± 0.09%) content in ethanol extract. In contrast, a higher total extraction value (22.8 ± 0.6%) and total terpenoid (57.23 ± 0.06 mg LL/g dw) content was shown in the methanol extract of A. parviflorum. LC–MS/MS-QTOF analysis of its ethanolic extract revealed a notable occurrence of phenols and flavonoids. The ethanolic extract of A. parviflorum exhibited significant antioxidant activities with lower IC50 values in DPPH, phosphomolybdenum, and metal chelating and reducing power assay. The methanolic extract of A. parviflorum had the more significant anti-inflammatory property (94.55 ± 0.1%) in the bovine serum albumin assay. The extracts also demonstrated a higher inhibition zone against pathogenic bacteria. The ethanolic extract of A. parviflorum demonstrated substantial cytotoxicity against A549 cells. Conclusion: Consequently, these findings validate the use of A. parviflorum in traditional medical practices due to its bioactive compounds, which may have potential therapeutic value in various biomedical applications. Highlights: A. parviflorum leaves extract showed the presence of significant levels of alkaloids, flavonoids, phenols, and terpenoids. LC–MS/MS-QTOF analysis revealed notable bioactive compounds of A. parviflorum. The different extracts showed remarkable antioxidant and antimicrobial activities. The extracts exhibited less hemolytic and higher thrombolytic activities. It demonstrated significant cytotoxicity against A549 cell lines. [ABSTRACT FROM AUTHOR]

Jackfruit seeds, often dismissed as by-products, are a powerhouse of nutrients and bioactive compounds. This comprehensive review explores various aspects of jackfruit seeds, including their nutritional composition, bioactive compounds, and health-promoting properties. Jackfruit seeds are a rich source of starch (12.86–17.90 g/100 g), protein (18%), fiber (1.56–3.92 g/100 g), minerals, and bioactive compounds such as phenolics, flavonoids, and saponins. These bioactive compounds have been found to protect against oxidative stress-related diseases. Moreover, jackfruit seeds demonstrate promising health effects, including antidiabetic, anticancer, hepatoprotective, and antimicrobial properties. Incorporating jackfruit seed flour in bakery products enhances their nutritional profiles and improves their functional properties, such as high oil and water absorption capacity. Comprehending the nutritional profile, health-promoting attributes, and functional characteristics of jackfruit seeds holds paramount importance. This knowledge serves as a cornerstone for advancing the creation of bakery food items endowed with enriched nutritional profiles and promising health benefits, which could be of significant interest to the food industry. [ABSTRACT FROM AUTHOR]

Sargassum is exploited as a biostimulant in agriculture for its rich bioactive compounds. Extracts from Sargassum promote plant growth, nutrient uptake, and stress tolerance in agricultural and horticultural crops. Sargassum is a most abundant seaweed, and it occupies a huge proportion of coastal plants. This review unveils key active constituents present in Sargassum such as proximate and ultimate compounds, antioxidants, phytohormones, glucosinoids, bioactive compounds, and enzymes. As a biostimulant, Sargassum mitigates biotic and abiotic stresses. It has a positive impact on soil health, plant physiology, crop yield, animal antimicrobials, and animal feeding, as well as highlighting some sustainable agricultural practices discussed in the review. Integrating Sargassum-based biostimulants in agriculture holds promise for resilient and productive crops with minimal environmental impact. These characteristics are considered the future of ideal agrochemical properties in all dimensions of plant growth and development. These kinds of organic-based biostimulants will be ideal alternatives to toxic chemical agents. [ABSTRACT FROM AUTHOR]

Summary Onion skin is generated in high amounts as food waste that is rich in bioactive components. Thus, the valorisation of onion skin waste in food production following suitable methods of treatment can be regarded as a sustainable strategy in terms of nutrition, economy, and environment. Skin waste from onions (Allium cepa) was ground and exposed to dielectric barrier discharge cold plasma (DBDCP) at 40 kV for either 0 or 30 min. Then the cold plasma‐treated onion skin powder (OSP) was added to chocolate at a concentration of 0%, 1%, or 2% (w/w). The DBDCP‐induced changes in the microbial load, colour, water content, total phenolic content (TPC), and antioxidant activity of OSP were assessed. The TPC, antioxidant activity, and sensory properties of the chocolate samples added with OSP were also monitored. Cold plasma treatment increased the TPC and antioxidant activity of OSP, but it did not affect the water content, colour, and microbial load. The antioxidant activity and TPC of the chocolate samples incorporated with DBDCP‐treated OSP at a level of 2% were higher than the samples with or without untreated OSP. However, the samples with 2% DBDCP‐treated OSP demonstrated lower overall acceptability scores compared to samples without OSP. It can be said that cold plasma can be utilised in the treatment of onion skin waste and valorisation of cold plasma‐treated onion skin waste in the production of chocolate to improve its antioxidant properties. Future studies can focus on the enhancement of the sensory properties of onion skin‐added chocolate products. [ABSTRACT FROM AUTHOR]

Summary Neltuma ruscifolia, a neglected underutilised species (NUS) within the Fabaceae family, lacks formal commercial recognition, despite its nutritional richness and endemic status. The objective of this study was to evaluate the antioxidant and antiglycating properties, as well as to identify associated bioactive compounds, in extracts (ultrasound and agitation) of different grinding fractions of N. ruscifolia seeds. Bioactivity varied depending on the extraction procedure and milling fraction, with the highest value in ultrasound‐extracted residue (exocarp, mesocarp). Some additional polyphenolic compounds (like hydroxybenzoic/ellagic acids) only appeared upon ultrasound‐assisted extraction. It was possible to identify some polyphenols (such as chrysin, rutin, kaempferol and cinnamic, coumaric, protocatechuic, ellagic and caffeic acids) that were highly related to the bioactivity. The antioxidant, antiglycating, colourant and technological properties of the extracts different fractions of N. ruscifolia pods are mainly dependent on the selected milling fraction. The extraction method, in a second place, significantly affects the resulting quantity and the type of extracted compounds, defining their potential applications. [ABSTRACT FROM AUTHOR]

Summary Oil palm (Elaeis guineensis) is a major oil crop that significantly contributes to the Indonesia's and Malaysia's economic growth. However, oil palm processing for oil extraction generates substantial waste, such as palm kernel cake, palm‐pressed fibre, oil palm empty fruit bunches and palm oil mill effluent, which pose noticeable environmental challenges. This review explores green extraction techniques for palm waste processing, which include microwave‐assisted extraction, deep eutectic solvents, ultrasound‐assisted extraction, supercritical fluid extraction, enzyme‐assisted extraction and subcritical water extraction. The potential for industry scaling, mechanisms, applications and challenges of each technique used for oil palm waste are discussed. Microwave‐assisted extraction, ultrasound‐assisted extraction and supercritical fluid extraction are particularly promising for large‐scale production, while deep eutectic solvents paired with ultrasound‐assisted extraction show the potential to improve extraction efficiency. This review also reveals that green extraction techniques are sustainable alternative to conventional techniques by effectively recovering valuable compounds from waste with reduced environmental impact. Additionally, the diverse applications of bioactive compounds (vitamin E, phytosterols, phenolics, polyphenols and flavonoids) extracted from oil palm waste in food, pharmaceutical, cosmetic and other industries are also presented. Valuable components such as cellulose, dietary fibres and bioactive peptides derived from the waste can be applied in food coatings, high‐protein products and drug delivery systems. Biosorbents, biofuels and biodegradable products derived from the waste would also provide environmental benefits. Future research should focus on optimising the green extraction techniques for industrial use to promote responsible production and circular waste management to foster health and environmental safety. [ABSTRACT FROM AUTHOR]

The edible plant oils production is associated with the release of different types of by-products. The latter represent cheap and available substrates to produce valuable compounds, such as flavours and fragrances, biologically active compounds and bio-based polymers. Elizabethkingia meningoseptica Oleate hydratases (Em_OhyA) can selectively catalyze the conversion of unsaturated fatty acids, specifically oleic acid, into hydroxy fatty acids, which find different industrial applications. In this study, Design-of-experiment (DoE) strategy was used to screen and identify conditions for reaching high yields in the reaction carried out by Escherichia coli whole-cell carrying the recombinant enzyme Em_OhyA using Waste Cooking Oils (WCO)-derived free fatty acids (FFA) as substrate. The identified reaction conditions for high oleic acid conversion were also tested on untreated triglycerides-containing substrates, such as pomace oil, sunflower oil, olive oil and oil mill wastewater (OMW), combining the triglyceride hydrolysis by the lipase from Candida rugosa and the E. coli whole-cell containing Em_OhyA for the production of hydroxy fatty acids. When WCO, sunflower oil and OMW were used as substrate, the one-pot bioconversion led to an increase of oleic acid conversion compared to the standard reaction. This work highlights the efficiency of the DoE approach to screen and identify conditions for an enzymatic reaction for the production of industrially-relevant products. • Enzymatic production of hydroxy fatty acids for different application, such as flavors. • Screening of biocatalytic hydration of crude free fatty acids from waste cooking oils. • Design-of-experiment used to identify interactions between parameters. • Production of enzymatic cascade reactions. • Reproducibility of results on triglyceride-containing industrial feedstocks. [ABSTRACT FROM AUTHOR]

Background: Multidrug-resistant Klebsiella pneumoniae (MDR-KP) poses a significant global health threat due to its involvement in severe infections and high mortality rates. The emergence of MDR strains necessitates the exploration of alternative therapeutic strategies. Methods: K. pneumoniae isolates were obtained from human and animal sources. Antibacterial susceptibility testing was performed, followed by the evaluation of essential oil activity through inhibition zone, MIC, and MBC determinations. Checkerboard assays were conducted to assess synergistic effects with amikacin. Gene expression analysis and transmission electron microscopy were employed to elucidate the mechanisms of action. Molecular docking studies were performed to identify potential binding targets of bioactive compounds. Results: Klebsiella pneumoniae was isolated from 25 of the100 samples examined, representing a prevalence rate of 25%. All isolates were found to be multidrugresistant. Tea tree and thyme essential oils exhibited potent antibacterial activity and synergistic effects with amikacin. Notably, these combinations significantly downregulated the expression of key capsule virulence genes (wcaG, rmpA, magA, uge, and wabG), suggesting a novel mechanism for enhancing amikacin efficacy. Transmission electron microscopy revealed disrupted cell integrity in MDR-KP cells treated with the combinations. Molecular docking analysis identified Terpinen-4-ol, Farnesol, 1,4-Dihydroxy-p-menth-2-ene, and 7-Oxabicyclo [4.1.0] heptane as potential bioactive compounds responsible for the observed effects. Conclusion: By effectively combating MDR-KP, this research holds promise for reducing antibiotic resistance, improving treatment outcomes, and ultimately enhancing potential care. [ABSTRACT FROM AUTHOR]

Introduction: Citrus fruit is rich in important functional constituents such as flavonoids, phenolic acids terpenes and other functional substances that play an important role for treating clinical diseases or controlling major agricultural diseases and pests. Plant secondary metabolites have become one of the most important resources of novel lead compounds, especially young citrus fruits contain multiple functional substances. 'Orah', a type of citrus reticulata, is known for its fine appearance, productivity, delicious sweetness, late-maturing characteristics, and is widely cultivated in China. Fruit thinning and rootstock selection are commonly used agronomic measures in its production to ensure its quality and tree vigor. However, few studies have demonstrated the effects of these agronomic measures on the functional substances of 'Orah'. Methods: In this study, we used HPLC coupled with UV to detect the dynamic changes of fruit quality, 13 main flavonoids, 7 phenolic acids, 2 terpenes, synephrine and antioxidant capacity in both peel and pulp of citrus fruits grafted on four rootstocks (Red orange Citrus reticulata Blanco cv. red tangerine, Ziyang xiangcheng Citrus junos Sieb. ex Tanaka, Trifoliate orange Poncirus trifoliata L. Raf, and Carrizo citrange Citrus sinensis Osb.×P.trifoliate Raf) at six different developmental stages (from 90 DAF to 240 DAF). Results: The results indicated that rootstock can significantly affect the contents of functional constituents and antioxidant capacity in 'Orah'. Additionally, it was found that pruning at either 90 DAF (days after flowering) or 150 DAF produced the most favorable outcomes for extracting functional substances. We also identified rootstock 'Trifoliate orange' has the highest total soluble solids (TSS) and 'Ziyang xiangcheng' to be the optimal in terms of comprehensive sensory of fruit quality, while 'Red orange' and 'Ziyang xiangcheng' are optimal in terms of functional substance quality, and 'Red orange' excels in antioxidant capacity. Discussion: Overall, the findings demonstrate the important role of rootstocks and developmental stage in shaping fruit sensory quality and functional substance synthesis, providing valuable insights for guiding rootstock selection, determining thinning time, and utilizing pruned fruits in a more informed manner. [ABSTRACT FROM AUTHOR]

Ficus auriculata (Timla) is an underutilized wild fruit compared to the more commonly consumed fruit of the same genus, Ficus carica (Anjeer). This review focuses on the industrial and pharmaceutical benefits of F. auriculata while highlighting its potential as a valuable alternative to F. carica. Based on the vast literature found in Scopus database, both fruits have significant nutritional and medicinal value; however, due to the rarity and lack of widespread knowledge, F. auriculata still remains underutilized, unlike F. carica, which is well known and more popular in the human diet. In terms of nutrition, F. auriculata is superior in various aspects like protein (3–5.32%), magnesium (68.0 mg/100 g), potassium (329.0 mg/100 g), and iron (5.0 mg/100 g). As a result, it plays a pivotal role for addressing nutrient deficiencies. Besides, it has rich history of many noteworthy applications in traditional remedies which are also recognized in modern pharmaceutical research. This could be attributed to the presence of phytocompounds. Ficus auriculata needs to be promoted, not only as a nutritional addition to the plate but also as an economically viable fruit. Based on its considerable potential, it is possible that F. auriculata can also be used as an alternative to F. carica; however, further research and promotion of the benefits of F. auriculata could help to increase its utilization and provide additional health and economic benefits to communities. Moreover, omics technologies based comprehensive study of fruits can assist in the identification of bioactive metabolites, nutritional content, and possible health benefits. It offers insights into the genetic composition, gene expression patterns, protein activities, and metabolic pathways of fruits thereby revealing the mechanisms driving flavor, color, and disease resistance. [ABSTRACT FROM AUTHOR]

In swine farming, animals develop diseases that require the use of antibiotics. In-feed antibiotics as growth promoters have been banned due to the increasing concern of antimicrobial resistance. Seaweeds offer bioactive molecules with antibacterial and antioxidant properties. The aim was to estimate the in vitro properties of seaweed extracts: Ascophyllum nodosum (AN), Palmaria palmata (PP), Ulva lactuca (UL), and 1:1 mixes (ANPP, ANUL, PPUL). Escherichia coli strains were used to test for growth inhibitory activity, and chemical-based assays were performed for antioxidant properties. The treatments were 2 (with/without Escherichia coli) × 2 (F4 + and F18 +) × 5 doses (0, 1.44, 2.87, 5.75, 11.50, and 23.0 mg/mL). Bacteria were supplemented with seaweed extracts, and growth was monitored. The antioxidant activity was assessed with 6 doses (0, 1, 50, 100, 200, 500, and 600 mg/mL) × 6 compounds using two chemical assays. Data were evaluated through SAS. The results showed that AN and UL significantly inhibited (p < 0.05) the growth of F4 + and F18 +. PP and mixes did not display an inhibition of the bacteria growth. AN, PP, UL extracts, and mixes exhibited antioxidant activities, with AN showing the strongest dose–response. Thus, AN and UL seaweed extracts reveal promising antibacterial and antioxidant effects and may be candidates for in-feed additives. [ABSTRACT FROM AUTHOR]

Summary The effects of ultrasound (US) pretreatment combined with drying process on storage quality maintenance and bioactive compound accumulation of broccoli stems were investigated. Results indicated that US pretreatment (λ = 8.5 mm) delayed the decline of L* value and ascorbic acid content but inhibited the increase of a* and b* values, browning index, and microbial counts of fresh‐cut broccoli stems. Furthermore, the US application enhanced total phenolics content by activating phenylalanine ammonia‐lyase, cinnamate‐4‐hydroxylase, and 4‐coumarate‐CoA ligase in phenylpropane metabolism. Meanwhile, it also promoted γ‐aminobutyric acid (GABA) accumulation by upregulating activities of diamine oxidase, polyamine oxidase, and aminoaldehyde dehydrogenase in GABA shunt and polyamine degradation pathway, respectively, which in turn, accelerated the biosynthesis of GABA from polyamines and glutamate. In addition, compared with other three drying methods, the combined vacuum freeze drying (VFD) could further retain the sensory attributes and bioactive compounds to a maximum extent in dehydrated broccoli stems. Hence, current findings illustrated that US pretreatment combined with VFD process is beneficial for commercial quality maintenance and nutritional value enhancement, thus providing new perspectives on the comprehensive utilisation of broccoli stems. [ABSTRACT FROM AUTHOR]

Summary The total carotenoid amounts of the caper young shoot and buds debittered by sonication in brine, NaOH and tap water were reported to be between 19.99 (control) and 37.23 μg g−1 (water) to 4.36 (control) and 20.38 μg g−1 (NaOH), respectively. Total phenolic contents of sonicated caper young shoot and buds in brine, NaOH and tap water were specified to be between 34.96 (NaOH) and 299.84 mg gallic acid equivalent (GAE)/100 g (control) to 45.04 (NaOH) and 373.12 mg GAE/100 g (control), respectively. Radical scavenging activity values of sonicated young shoots were measured between 1.26 (NaOH) and 7.58 mmol trolox equivalent (TE) kg−1 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH) (control). In addition to DPPH, antioxidant activity values of sonicated caper young shoot and buds were measured to be between 2.06 (NaOH) and 17.72 Ferric reducing antioxidant power (FRAP), mg g−1 (control) to 1.70 (NaOH) and 13.44 FRAP mg g−1 (control), respectively. Kaempferol quantities of sonicated caper young shoot were identified between 40.08 (control) and 53.83 mg/100 g (Brine). In general, the most reduction in phenolic components was observed in caper young shoot and buds sonicated in NaOH solution. The phenolic components of the caper young shoot and buds sonicated in different solutions showed changes on the solution type when compared to the control. [ABSTRACT FROM AUTHOR]

Pomegranate juice (PJ) is rich in various bioactive compounds, including anthocyanins (ATs), ellagic tannins (ETs) and ellagic acid (EA), and has the potential to be utilized as a therapeutic intervention for iron deficiency anemia (IDA). This narrative review offers a comprehensive overview of current research on the efficacy of PJ in the prevention and treatment of IDA, while also delving further into associated mechanisms. It is proposed that PJ may effectively manage IDA through direct or indirect means, including acting as a source for iron supplementation, enhancing iron absorption, exerting antioxidant and anti-inflammatory effects, modulating gut microbiota and promoting intestinal mucosal repair. Nevertheless, more researches are warranted to validate the effectiveness and underlying mechanisms of PJ as a promising therapy for IDA. [ABSTRACT FROM AUTHOR]

Polycyclic heterocycles are the most common and critical structural motifs found in a variety of natural products, medicines, fertilizers, and advanced materials. Because of their widespread use in biologically active compounds and material chemistry, functionalised dibenzo heterocyclic compounds, especially dibenzofuran, dibenzothiophene, and carbazole derivatives, garnered much attention over time. Scientists are especially interested in elucidating more efficient techniques for developing these industrially essential compounds. Dibenzo‐fused heterocycles can rapidly be synthesised using highly efficient transition metal‐catalysed strategies as well as by economic metal‐free reaction conditions. This review includes a detailed overview of the most recent significant synthetic techniques, both metal‐catalysed and metal‐free, to produce these industrially significant and medicinally important dibenzo‐fused heterocycles. [ABSTRACT FROM AUTHOR]

Summary Edible mushrooms have become an important global ingredient due to their rich nutrients and delicious taste. However, their high moisture content, abundant bioactive components and susceptibility to microbial contamination led to a relatively short shelf‐life. Therefore, extensive research on the active ingredients, bioactivity and preservation techniques of mushrooms is crucial for enhancing the rapid development and increasing acceptance of the mushroom industry. Furthermore, the comprehensive utilisation of by‐products from mushroom processing and the diversified development of mushroom products are important means to increase the added value of the mushroom industry. Therefore, through the aforementioned research, the sustainable and healthy development of the mushroom industry can be promoted. [ABSTRACT FROM AUTHOR]

Fenugreek (Trigonella foenum‐graecum) is a widely grown dietary herb in Asia, and its seeds are traditionally used for several diseases, including diabetes. The seeds and leaves possess a variety of compounds that play an important role in regulating their hypoglycemic effect. However, so far, no extensive systematic review exists on its antidiabetic effect, highlighting the molecular mechanisms and isolated compounds. The purpose of this review is to summarize the preclinical and clinical antidiabetic properties of fenugreek and its isolated compounds by focusing on underlying mechanisms. PubMed, Google Scholar, Science Direct, and Scopus databases were searched to retrieve articles until June, 2024. Preclinical studies demonstrated that the antidiabetic effect of fenugreek was mostly associated with enhanced glucose transporter type‐4 (GLUT4) translocation and hexokinase activity, decreased glucose‐6‐phosphatase and fructose‐1,6‐bisphosphatase activities, inhibited α‐amylase and maltase activities, protected β cells, and increased insulin release. Furthermore, few studies have reported its role as a glucagon‐like peptide‐1 (GLP‐1) modulator, 5′‐AMP‐activated kinase (AMPK) activator, and dipeptidyl peptidase‐IV (DPP‐IV) inhibitor. Further clinical trials showed that fenugreek seeds improved blood glucose levels, insulin resistance, insulin sensitivity, and lipid profiles. This study highlights significant evidence of the antidiabetic effect of fenugreek and its isolated compounds; therefore, it could be a potential therapy for diabetes. [ABSTRACT FROM AUTHOR]

Natural products have been used since ancient times to treat various ailments and have been recognized for many years as a source of therapeutic agents and structural diversity. Plant-derived products have thus served as dietary components but also to maintain a state of wellbeing and health by preventing different diseases both of inflammatory and infective nature. Pentacyclic triterpenoids, particularly ursolic acid (UA) and oleanolic acid (OA), are well-studied natural products endowed with complex biological profiles. In this mini-review, we summarized the most advanced results on extraction methodologies and antimicrobial activity of UA and OA, focusing on their potential role as antimicrobic adjuvants, bacterial biofilm inhibitors and related mechanisms of action. This offers a theoretical basis and inspiration for further studies on their bioactivity mechanism. [ABSTRACT FROM AUTHOR]

This study investigated the proximate composition and inhibitory potential of hot water and ethanolic extracts of the pulp, peel and whole fruit of green banana (Musa sapientum) on α‐amylase and α‐glucosidase. Bioactive compounds were identified using GC‐MS analysis. In addition, the cytotoxic effect on human gingival fibroblast (hGF) was evaluated using the sulphorhodamine B (SRB) assay. The results showed that the peel of green banana had the highest amount of ash (10.05%), fat (2.83%), protein (3.64%) and total dietary fibre (36.62%). The carbohydrate content of the whole fruit (81.79%) and pulp (81.50%) was higher than that of the peel (71.90%). The moisture content of the pulp (13.08%) was higher than that of the peel (11.58%) and whole fruit (11.30%). The ethanolic green banana peel extract showed a good inhibitory effect of α‐amylase and α‐glucosidase with the concentration necessary for 50% inhibition (IC50) of 0.512 and 0.100 mg·mL−1, respectively. The α‐glucosidase inhibitory effect of the ethanolic green banana peel extract and the hot water green banana peel extract was not significantly different from that of acarbose (IC50 0.108 mg·mL−1). GC‐MS analysis of the ethanolic green banana peel extract revealed fatty acids and fatty acid ester (9‐octadecenamide (Z), octadecanamide and other compounds). The ethanolic peel extract exhibits a significant noncytotoxicity effect on hGF cells at concentrations ranging from 0.0001 to 1.0 mg·mL−1. [ABSTRACT FROM AUTHOR]

Digitalis purpurea L. is one of the important plant species of Nilgiris, Kashmir and Darjeeling regions of India, belonging to the family Plantaginaceae, with well-known pharmacological applications. In the present investigation, an in vitro culture technique of indirect shoot organogenesis of D. purpurea is being explored; the biochemical attributes, the antioxidant activities and the metabolomic analyses were made by utilizing untargeted Gas Chromatography-Mass Spectrometry (GC-MS) and Ultra Performance Liquid Chromatography coupled with electronspray ionization/quadrupole-time-of-flight-mass spectrometry (UPLC-ESI-QTOF-MS) approaches. Initially, the leaf explants were used for callus induction and proliferation and maximum callusing frequency (94.44%) and fresh biomass (4.9 g) were obtained on MS, fortified with 8.8 µM BAP (6-benzyl amino purine) + 0.9 µM 2,4-D (2,4-dichlorophenoxyacetic acid), subsequently shoot formation (indirect organogenesis) was noted on the same MS medium with a shoot induction frequency of 83.33%. Later on, the biochemical and antioxidant potential of in vivo-, in vitro grown leaf and leaf derived callus were assessed. Significantly higher total phenol, flavonoid, DPPH (2,2-diphenyl-1-picrylhydrazyl), POD (peroxidase) and SOD (superoxide dismutase) activities were noticed in in vitro grown callus and leaf tissues compared with field grown leaf. The GC-MS analysis of each methanolic extract (in vivo-, in vitro derived leaf and leaf derived callus) displayed the presence of more than 75 bioactive compounds viz loliolide, stigmasterin, alpha-tocopherol, squalene, palmitic acid, linoleic acid, beta-amyrin, campesterol etc. possessing immense therapeutic importance. The UPLC-MS based metabolite fingerprinting of each methanolic extracts were conducted in both positive and negative ionization mode. The obtained results revealed variation in phytochemical composition in field - and laboratory grown tissues, indicating the impact of in vitro culture conditions on plant tissues. The detected phytocompounds belongs to various classes such as flavonoids, steroids, terpenoids, carbohydrates, tannins, lignans etc. The medicinally important metabolites identified were 20, 22-dihydrodigoxigenin, digoxigenin monodigitoxoside, apigenin, luteolin, kaempferide, rosmarinic acid, nepitrin and others. The results of the present study suggest that in vitro culture of D. purpurea could successfully be utilized for the novel drug discovery by producing such important phytocompounds of commercial interest in shorter duration without harming the plants' natural population. [ABSTRACT FROM AUTHOR]

Summary The instant controlled pressure drop (DIC) technology represents a highly efficient and eco‐friendly process that enhances the texture of food matrices. In this study, oleaster flour (OF) was subjected to DIC treatment at varying pressures (200 and 500 kPa) and processing times (30, 60, and 120 s) for the purpose of analysing its techno‐functional, chemical, concentration of bioactive components, and structural properties. The results demonstrated statistically significant differences between the untreated and DIC‐treated OF samples (P < 0.05). The application of DIC treatment resulted in a notable enhancement in the water activity, fat, ash, SDF, bound phenolic content, and antioxidant capacity of the OF (P < 0.05). The mineral content and structural properties were analysed in two samples of OF (DIC‐treated at 200 and 500 kPa for 60 s), which were selected for their superior bioactive components in comparison to the untreated OF. The 200 and 500‐kPa 60‐s DIC treatment also enhanced the mineral (Cu, Na, Mn, Mg, P, Ca, Fe, and K) and structural properties of OF, thereby rendering it a more functional flour. The utilisation of the DIC method enhances the overall quality of the end product, resulting in a visually acceptable appearance, an extended shelf life and heightened nutritional content, thereby rendering the product more practical and convenient. [ABSTRACT FROM AUTHOR]

BACKGROUND RESULTS CONCLUSION Michelia macclurei Dandy is a traditional Chinese medicinal plant, but little is understood about the bioactive compositions and biological potential of its different parts, limiting their applications. This study aims to identify the bioactive compositions and analyze differences in accumulation patterns from different parts of Michelia macclurei (heartwood, sapwood, bark, root, leaf, and fruit) using metabolomics. It also seeks to explore their biological potential and analyze the relationship between the bioactive compositions and biological potential.A total of 63 volatile metabolites (VMs) were identified by gas chromatography–mass spectrometry (GC–MS) in six parts, and the VMs in each part were dominated by sesquiterpenes and their derivatives (71.40–88.32%). Six parts of Michelia macclurei contained structurally diverse non‐volatile metabolites (NVMs) with a total of 207 bioactive compounds, including 92 alkaloids, 30 flavonoids, 19 lignans, and 18 organic acids, utilizing ultra‐performance liquid chromatography–mass spectrometry (UPLC–MS) analysis. Multivariate statistical analysis showed that the accumulation patterns of bioactive compositions differed significantly among the different parts, and the 25 VMs and 72 NVMs could be considered potential markers for distinguishing the different parts of Michelia macclurei. The excellent antioxidant and enzyme inhibitory capacity of extracts of all six parts was indicated by in vitro bioactivity assays. Pearson's correlation analysis showed that the bioactive compositions in the six parts were significantly correlated with antioxidant and enzyme inhibitory activities.This study offers helpful information on the distribution of bioactive compositions in different parts of Michelia macclurei and confirms the excellent antioxidant, and enzyme inhibitory potential of its extracts, which could provide scientific evidence for its potential applications in the pharmaceutical industry, cosmetics, and functional foods. © 2024 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

Background Results Conclusion Carotenoids have various physiological functions, such as immune regulation and cancer prevention. Germination could further improve the content of carotenoids in maize seeds. In this study, yellow maize seeds (Suyu 29) were soaked and germinated with different concentrations of 24‐epibrassinolide. The changes of germination percentage, sprout length, bioactive components, antioxidant capacity and carotenoid content of the maize seeds were analyzed. Additionally, the relative expression of key genes in the carotenoid synthesis pathway was investigated.The results showed that the sprout length, germination percentage, soluble protein, free amino acids, proline, endogenous abscisic acid, vitamin C, total phenolics and carotenoids displayed a significant increasing trend compared with the control group (P < 0.05). The activity of superoxide dismutase and peroxidase increased by 55.1% and 58.5% versus the control group, and the antioxidant capacity of 2,2‐diphenyl‐1‐picrylhydrazyl, 2,2‐azinobis(3‐ethylbenzothiazoline‐6‐sulfonic acid) and ferric reducing antioxidant power was 19.8%, 13.4% and 44.1% higher than that of the control group (P < 0.05). Compared with the control group, the expression of genes was significantly up‐regulated (P < 0.05). Under the treatment of 0.1 mg L−1 of 24‐epibrassinolide, carotenoid content reached the highest value. The carotenoids showed a positive correspondence with antioxidant enzyme activity, antioxidant capacity and total phenolics content (P < 0.05).This study showed that 0.1 mg L−1 of exogenous 24‐epibrassinolide promoted the accumulation of carotenoids and improved the antioxidant capacity and the quality of germinated maize seeds. It could provide a method for the development of germinated maize products enriched in carotenoids. © 2024 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

Mauritia flexuosa (M. flexuosa), commonly known as Aguaje or Moriche palm, is traditionally recognised in South America for its medicinal properties, particularly for its anti-inflammatory and antioxidant effects. However, the bioactive compounds responsible for these effects have not been thoroughly investigated. This study aims to isolate and characterise pentacyclic triterpenoid compounds from M. flexuosa and to evaluate their therapeutic potential. Using various chromatographic and spectroscopic techniques including Nuclear Magnetic Resonance (NMR) and Mass Spectrometry (MS), three pentacyclic triterpenoid compounds were successfully isolated. Among them, compound 1 (3,11-dioxours-12-en-28-oic acid) exhibited notable bioactivity, significantly inhibiting the activation of Nuclear Factor kappa-light-chain-enhancer of activated B cells (NF-κB) (IC50 = 7.39–8.11 μM) and of Nitric Oxide (NO) (IC50 = 4.75–6.59 μM), both of which are key processes in inflammation. Additionally, compound 1 demonstrated potent antioxidant properties by activating the antioxidant enzyme Superoxide Dismutase (SOD) (EC50 = 1.87 μM) and the transcription factor Nuclear factor erythroid 2-related factor 2 (Nrf2) (EC50 = 243–547.59 nM), thus showing its potential in combating oxidative stress. This study is the first to isolate and characterise the three compounds from M. flexuosa, suggesting that compound 1 could be a promising candidate for the development of safer and more effective therapies for inflammatory and oxidative stress-related diseases. [ABSTRACT FROM AUTHOR]

Cyclic organic compounds containing sulfur atoms constitute a large group, and they play an important role in the chemistry of heterocyclic compounds. They are valuable intermediates for the synthesis of other compounds or biologically active compounds themselves. The synthesis of heterocyclic compounds poses a major challenge for organic chemists, especially in the context of applying the principles of "green chemistry". This work is a review of the methods of synthesis of various S-heterocyclic compounds using green solvents such as water, ionic liquids, deep eutectic solvents, glycerol, ethylene glycol, polyethylene glycol, and sabinene. The syntheses of five-, six-, and seven-membered heterocyclic compounds containing a sulfur atom or atoms, as well as those with other heteroatoms and fused-ring systems, are described. It is shown that using green solvents determines the attractiveness of conditions for many reactions; for others, such use constitutes a real compromise between efficiency and mild reaction conditions. [ABSTRACT FROM AUTHOR]

Marine algal toxins have garnered significant attention in the research community for their unique biochemical properties and potential medical applications. These bioactive compounds, produced by microalgae, pose significant risks due to their high toxicity, yet offer promising therapeutic benefits. Despite extensive research identifying over 300 marine algal toxins, including azaspiracids, brevetoxins, cyclic imines, and yessotoxins, gaps remain in the understanding of their pharmacological potential. In this paper, we critically review the classification, bioactive components, toxicology, pharmacological activities, and mechanisms of these toxins, with a particular focus on their clinical applications. Our motivation stems from the increasing interest in marine algal toxins as candidates for drug development, driven by their high specificity and affinity for various biological receptors. We aim to bridge the gap between toxicological research and therapeutic application, offering insights into the advantages and limitations of these compounds in comparison to other bioactive substances. This review not only enhances the understanding of marine algal toxins' complexity and diversity, but also highlights their extensive application potential in medicine and bioscience, providing a foundation for future research and development in this field. [ABSTRACT FROM AUTHOR]

Calendula officinalis L. has numerous health-promoting properties due to the presence of a large number of lipophilic compounds. Their effective delivery to the body requires the use of an appropriate technique such as emulsification. So, the main purpose of this study was to understand how the profile of lipophilic compounds from pot marigold and the pro-health potential are shaped by different types of protein, oil, and drying techniques in o/w nanoemulsion. To obtain this, the profiles of carotenoid compounds and tocols were measured. Additionally, antioxidant potential and the ability to inhibit α-amylase and α-glucosidase were measured. Pea protein emulsion exhibited a higher final content of carotenoid compounds (23.72–39.74 mg/100 g), whereas those with whey protein had stronger α-amylase inhibition (487.70 mg/mL). The predominant compounds in the studied nanoemulsions were β-carotene (between 19% and 40%), followed by α-tocopherol/γ-tocopherol. The type of proteins shaped the health-promoting properties and determined the content of health-promoting compounds. [ABSTRACT FROM AUTHOR]

Botanical varieties of hemp differ in chemical composition, plant morphology, agronomy, and industrial suitability. Hemp is popular for cultivation for the production of cannabinoid oil, fiber production, biomass, etc. The fertilization process is one of the most important factors affecting the plant, both its condition and chemical composition. So far, research has been carried out proving that hemp is a valuable source of, among others: fatty acids, amino acids, acids, vitamins, numerous micro- and macroelements, and antioxidant compounds. In this experiment, it was decided to check the possibility of harvesting hemp panicles twice in one year. The purpose of this treatment is to use one plant to produce cannabidiol oil and grain. The main aim of the research was to determine bioactive compounds in hemp seeds and to determine whether the cultivation method affects their content and quantity. Based on the research conducted, it was observed that hemp can be grown in two directions at the same time and harvested twice because its health-promoting properties do not lose their value. It was found that regardless of whether hemp is grown solely for seeds or to obtain essential oils and then seeds, the type of fertilization does not affect the content of phenolic acids (e.g., syringic acid: 69.69–75.14 μg/100 g, vanillic acid: 1.47–1.63 μg/100 g). Based on the conducted research, it was found that essential oils can be obtained from one plant in the summer and seeds from Henola hemp cultivation in the autumn, because such a treatment does not affect the content of the discussed compounds. [ABSTRACT FROM AUTHOR]

Ethanolic extracts of Baikal skullcap (Scutellaria baicalensis) root were obtained using various techniques, such as maceration, maceration with shaking, ultrasound-assisted extraction, reflux extraction, and Soxhlet extraction. The influence of the type and time of isolation technique on the extraction process was studied, and the quality of the obtained extracts was determined by spectrophotometric and chromatographic methods to find the optimal extraction conditions. Radical scavenging activity of the extracts was analyzed using DPPH assay, while total phenolic content (TPC) was analyzed by the method with the Folin–Ciocalteu reagent. Application of gas chromatography with mass selective detector (GC-MS) enabled the identification of some bioactive substances and a comparison of the composition of the particular extracts. The Baikal skullcap root extracts characterized by both the highest antioxidant activity and content of phenolic compounds were obtained in 2 h of reflux and Soxhlet extraction. The main biologically active compounds identified in extracts by the GC-MS method were wogonin and oroxylin A, known for their broad spectrum of biological effects, including antioxidant, anti-inflammatory, antiviral, anticancer, and others. [ABSTRACT FROM AUTHOR]

Natural plant exosomes are transforming the landscape of skin and hair care with their remarkable bioactive properties. These small extracellular vesicles (EVs), secreted by plant cells, are rich in DNA, RNA, proteins, and lipids, acting as natural biological communicators. Unlike synthetic alternatives, plant exosomes offer a sustainable, natural, and highly effective solution, aligning with the growing consumer demand for eco-friendly and efficacious beauty products. By facilitating cross-kingdom communication, these plant-derived exosomes introduce a new era in cosmetic innovation, offering a unique and potent approach to enhancing skin and hair health. The ability to customize and guide the production of different types of exosomes further underscores their potential, making natural plant exosomes a groundbreaking advancement in the pursuit of advanced and sustainable cosmetic solutions. By leveraging sustainable and innovative biotechnology, companies like Vytrus Biotech are pioneering the use of plant exosomes, offering a natural and effective alternative to synthetic cosmetic ingredients. The biotech company has harnessed this potential by developing high-concentration plant-derived exosomes from Curcuma longa and Centella asiatica cell cultures. These exosomes significantly enhance skin hydration, regeneration, and hair growth, effectively promoting anti-aging, anti-inflammatory, and regenerative effects, making them powerful allies in cosmetic formulations. [ABSTRACT FROM AUTHOR]

For millennia, people have successfully treated a wide range of illnesses, including bacterial infections, with different plant parts or extracts. "MDR strain" typically refers to a "multi-drug resistant strain" of a bacteria. In the context of infectious diseases, a multi-drug resistant strain refers to a strain of the pathogen that has acquired resistance to multiple drugs that are commonly used to treat infections caused by that specific pathogen. MDR strains can present significant challenges in healthcare settings as they limit the effectiveness of standard treatments and may require more aggressive or specialized approaches to manage the infection. The discdiffusion method was used in this investigation to test the antimicrobial properties of Petroleum ether, acetone, ethanol, methanol, and aqueous extracts of Ficus auriculata leaves against four bacterial strains namely Salmonella enteric serovar typhi, Salmonella enteric ser Paratyphi (MDR strain) Pseudomonas aeruginosa and Escherichia coli. The findings showed that all of the investigated organisms (zone of inhibition of 0.5 ± 0.15 & 18 ± 1.7 mm) were significantly inhibited by the petroleum ether, ethanol, and methanol extracts, with the exception of Salmonella typhi (an 18 mm inhibitory zone). The restricted area (=5) indicated moderate activity in the aqueous extracts. It's crucial to remember that antimicrobial activity analysis of plant extracts is just one step in the process of identifying potential natural antimicrobial agents. Further studies, including the identification and isolation of specific bioactive compounds, toxicology assessments, and clinical trials, are required before any plant extract can be considered for use as a drug development. [ABSTRACT FROM AUTHOR]

Utilizing vegetable waste to produce bioactive compounds through microbial consortia represents a holistic solution to waste management challenges. Efficient waste collection systems in major cities ensure proper segregation of vegetable waste and lay the groundwork for resource utilization. Segregating waste at its source enhances waste stream quality and facilitates downstream processing. Research and development efforts investigating tailored microbial consortia seek to optimize waste degradation and bioactive compound yields, thereby unlocking the potential of waste. This approach significantly reduces greenhouse gas emissions by diverting vegetable waste from landfills, thereby mitigating climate change effects and improving air quality. Moreover, it conserves natural resources by reducing the need for virgin materials and promoting biodiversity, conservation, and sustainable resource management. Here, the enzymatic activities and phenolic concentrations derived from the biodegradation of vegetable waste were analyzed over 14 days. Protease activity that plays a vital role in breaking down proteins reached a notable level of 300 µg/ml after 14-day incubation. Lipase activity which is essential for lipid breakdown was observed at a concentration of 6.3 µg/ml. Furthermore, phenolic concentration analysis revealed a significant range, with values ranging from 225 µg/ml to 240 µg/ml after 14 days of incubation. Phenolics are phytochemicals possessing antioxidant properties and potential health benefits. These findings provide valuable insight into the efficacy of utilizing vegetable waste as a substrate for enzymatic and phytochemical production. The observed enzymatic activities and phenolic concentrations highlight the potential of vegetable waste as a valuable resource for the sustainable production of industrially relevant compounds. [ABSTRACT FROM AUTHOR]

Weeds reduce the crops yield and quality of produce. We identified biomolecules and quantified the phenolics and flavonoids contents in the Amaranthus viridis L. weed extracts. The antifungal activity of its extracts was determined against Fusarium (F. oxysporum f.sp ciceri, F. oxysporum f. sp. chlamydosporum, F. oxysporum f.sp. pallidoroseum and F. oxysporum f. sp. vasinfectum) species. We found that A. viridis bioactive components bioactivity can be used to controls pathogens. Thus, we identified various bioactive components of this weed and studied their activity, and role in activating defence in plants. [ABSTRACT FROM AUTHOR]

Celosia cristata (family Amaranthaceae), has significant medicinal value with rich phytochemical composition and is used traditionally across cultures, it treats various ailments due to its diverse bioactive compounds (sterols, tannins, saponins, and flavonoids). It demonstrates pharmacological capability: anti-inflammatory, hepatoprotective and antiviral effects. The methanolic extracts of C. cristata flower contains alkaloids, tannins, carbohydrate, saponins, terpenoids, phenols, flavonoids, and steroids. These bioactive phytochemical compounds in methanolic extracts, supports its traditional medicinal use for various ailments. The abundant presence of phytochemicals in C. cristata flowers suggests their potential application in pharmaceutical industries for drug development. These findings provide a scientific basis for the traditional medicinal use of C. cristata in managing ailments and disorders. [ABSTRACT FROM AUTHOR]