1. Human adaptation to immobilization:Novel insights of impacts on glucose disposal and fuel utilization
- Author
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Natalie F. Shur, Elizabeth J. Simpson, Hannah Crossland, Prince K. Chivaka, Despina Constantin, Sally M. Cordon, Dumitru Constantin‐Teodosiu, Francis B. Stephens, Dileep N. Lobo, Nate Szewczyk, Marco Narici, Clara Prats, Ian A. Macdonald, Paul L. Greenhaff, Greenhaff, Paul, and Simpson, Liz
- Subjects
Bio/Medical/Health - Physiology ,Arthritis Research UK Centre for Sport Exercise and Osteoarthritis ,Physiology (medical) ,bed rest ,fuel oxidation ,insulin resistance ,muscle metabolism ,Orthopedics and Sports Medicine ,NIHR Nottingham Hearing Biomedical Research Unit - Abstract
Background: Bed rest (BR) reduces whole-body insulin-stimulated glucose disposal (GD) and alters muscle fuel metabolism, but little is known about metabolic adaptation from acute to chronic BR nor the mechanisms involved, particularly when volunteers are maintained in energy balance. Methods: Healthy males (n = 10, 24.0 ± 1.3 years), maintained in energy balance, underwent 3-day BR (acute BR). A second cohort matched for sex and body mass index (n = 20, 34.2 ± 1.8 years) underwent 56-day BR (chronic BR). A hyperinsulinaemic euglycaemic clamp (60 mU/m2/min) was performed to determine rates of whole-body insulin-stimulated GD before and after BR (normalized to lean body mass). Indirect calorimetry was performed before and during steady state of each clamp to calculate rates of whole-body fuel oxidation. Muscle biopsies were taken to determine muscle glycogen, metabolite and intramyocellular lipid (IMCL) contents, and the expression of 191 mRNA targets before and after BR. Two-way repeated measures analysis of variance was used to detect differences in endpoint measures. Results: Acute BR reduced insulin-mediated GD (Pre 11.5 ± 0.7 vs. Post 9.3 ± 0.6 mg/kg/min, P
- Published
- 2022
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