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111 results on '"Bino, R. J."'

33. ß-Tubulin accumulation and DNA synthesis are sequentially resumed in embryo organs of cucumber (Cucumis sativus L.) seeds during germination

Author

Jing, H. -C, Lammeren, A. A. M., Castro, R. D., Bino, R. J., Hilhorst, H. W. M., and Steven P.C. Groot

Subjects
Laboratory of Plant Cell Biology, Laboratorium voor Plantencelbiologie, Bromodeoxyuridine incorporation, β-Tubulin accumulation, DNA synthesis, Plant Research International, Microtubular cytoskeleton, Germination, EPS, Cucumis sativus
Published
1999

45. Characterization of ferric reducing activity in roots of Fe-deficient Phaseolus vulgaris.

Author

Blienfait, H. F., Bino, R. J., Van Der Bliek, A. M., Duivenvourden, J. F., and Fontaine, S. M.

Subjects
*COMMON bean, *PLANT roots, *IRON, *CELL membranes, *BOTANY, *ENZYMES
Abstract

Iron‐deficient bean plants (Phaseolus vulgaris L. cv. Prélude) exhibited a ferric reducing activity in the roots, with kinetics characteristic for matrix‐bound enzymes: the reaction rate was proportional to substrate (Fe‐EDTA) concentration until 100 μM, and at higher concentrations it leveled off to a maximum; the Lineweaver‐Burk plot yielded a non‐linear relationship between rate −1 and substrate −1. The Arrhenius plot yielded apparent activation energies which were dependent on substrate concentration. No evidence was obtained for the secretion by roots of a low molecular weight metabolite involved in the reduction of iron prior to its uptake. The results are interpreted to indicate that the ferric reducing activity in the roots of iron‐deficient bean plants is located in an enzyme in the plasmalemma of the cortex or epidermis cells. [ABSTRACT FROM AUTHOR]

Published
1983
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48. Effects of Temperature on Pollen Tube Growth and Fruit Set in Reciprocal Crosses Between <em>Cucumis sativus</em> and <em>C. metuliferus</em>.

Author

Franken, J., Custers, J. B. M., and Bino, R. J.

Subjects
CUCUMBERS, SPECIES hybridization, TEMPERATURE, PLANT embryology, OVULES, PLANT reproduction
Abstract

Copyright of Plant Breeding is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Published
1988
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50. Cell division and subsequent radicle protrusion in tomato seeds are inhibited by osmotic stress but DNA synthesis and formation of microtubular cytoskeleton are not.

Author

de Castro, R D, van Lammeren, A A, Groot, S P, Bino, R J, and Hilhorst, H W

Abstract

We studied cell cycle events in embryos of tomato (Lycopersicon esculentum Mill. cv Moneymaker) seeds during imbibition in water and during osmoconditioning ("priming") using both quantitative and cytological analysis of DNA synthesis and beta-tubulin accumulation. Most embryonic nuclei of dry, untreated control seeds were arrested in the G(1) phase of the cell cycle. This indicated the absence of DNA synthesis (the S-phase), as confirmed by the absence of bromodeoxyuridine incorporation. In addition, beta-tubulin was not detected on western blots and microtubules were not present. During imbibition in water, DNA synthesis was activated in the radicle tip and then spread toward the cotyledons, resulting in an increase in the number of nuclei in G(2). Concomitantly, beta-tubulin accumulated and was assembled into microtubular cytoskeleton networks. Both of these cell cycle events preceded cell expansion and division and subsequent growth of the radicle through the seed coat. The activation of DNA synthesis and the formation of microtubular cytoskeleton networks were also observed throughout the embryo when seeds were osmoconditioned. However, this pre-activation of the cell cycle appeared to become arrested in the G(2) phase since no mitosis was observed. The pre-activation of cell cycle events in osmoconditioned seeds appeared to be correlated with enhanced germination performance during re-imbibition in water.

Published
2000
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