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Abstract Background Highly active anti-retroviral therapy (HAART) can successfully suppress human immunodeficiency virus (HIV) viral replication and reconstruct immune function reconstruction in HIV-1-infected patients. However, about 15–30% of HIV-1-infected patients still fail to recover their CD4+ T cell counts after HAART treatment, which means immune reconstruction failure. Pyroptosis plays an important role in the death of CD4+ T cells in HIV-1- infected patients. The study aims to explore the association between the expression of pyroptosis in peripheral blood and immune function reconstruction in HIV-1- infected patients. Methods One hundred thirty-five HIV-1-infected patients including immunological non-responders (INR) group, immunological responders (IR) group and normal immune function control (NC) group were analyzed. The expression of GSDMD and Caspase-1 in peripheral blood of HIV-1-infected patients were measured by qPCR. The concentrations of GSDMD, Caspase-1, IL-1β and IL-18 in the peripheral serum were quantified by ELISA. The associations between the expression of pyroptosis in peripheral blood and immune function reconstruction were analyzed using multivariate logistic models. Results The relative expression of GSDMD mRNA and caspase-1 mRNA in peripheral blood, as well as the expression of IL-18 cytokine in the INR, were significantly higher than those in the IR and NC (P 0.05). Multivariate logistic analysis showed that the patients with baseline CD4+ T cell counts less than 100 cells/μL (aOR 7.051, 95% CI 1.115–44.592, P = 0.038), high level of expression of Caspase-1mRNA (aOR 2.803, 95% CI 1.065–7.377, P = 0.037) and IL-18 cytokine (aOR 10.131, 95% CI 1.616–63.505, P = 0.013) had significant poor CD4+ T cell recovery. Conclusions The baseline CD4+ T cell counts less than 100 cells/μL, high relative expression of Caspase-1 mRNA, and high expression of IL-18 cytokine are associated factors that affect the reconstruction of immune function.

Chronic wounds and the accompanying inflammation are ongoing challenges in clinical treatment. They are usually accompanied by low pH and high oxidative stress environments, limiting cell growth and proliferation. Ordinary medical gauze has limited therapeutic effects on chronic wounds, and there is active research to develop new wound dressings. The chitosan hydrogel could be widely used in biomedical science with great biocompatibility, but the low mechanical properties limit its development. This work uses polyacrylamide to prepare double-network (DN) hydrogels based on bioadhesive catechol-chitosan hydrogels. Cystamine and N, N′-Bis(acryloyl)cystamine, which can be cross-linking agents with disulfide bonds to prepare redox-responsive DN hydrogels and pH-responsive nanoparticles (NPs) prepared by acetalized cyclodextrin (ACD) are used to intelligently release drugs against chronic inflammation microenvironments. The addition of catechol groups and ACD-NPs loaded with the Resolvin E1 (RvE1), promotes cell adhesion and regulates the inflammatory response at the wound site. The preparation of the DN hydrogel in this study can be used to treat and regulate the inflammatory microenvironment of chronic wounds accurately. It provides new ideas for using inflammation resolving factor loaded in DN hydrogel of good biocompatibility with enhanced mechanical properties to intelligent regulate the wound inflammation and promote the wound repaired.

Coronary atherosclerotic lesions exhibit a low-pH chronic inflammatory response. Due to insufficient drug release control, drug-eluting stent intervention can lead to delayed endothelialization, advanced thrombosis, and unprecise treatment. In this study, hyaluronic acid and chitosan were used to prepare pH-responsive self-assembling films. The hydrogen sulfide (H2S) releasing aspirin derivative ACS14 was used as drug in the film. The film regulates the release of the drug adjusted to the microenvironment of the lesion, and the drug balances the vascular function by releasing the regulating gas H2S, which comparably to NO promotes the self-healing capacity of blood vessels. Drug releasing profiles of the films at different pH, and other biological effects on blood vessels were evaluated through blood compatibility, cellular, and implantation experiments. This novel method of self-assembled films which H2S in an amount, which is adjusted to the condition of the lesion provides a new concept for the treatment of cardiovascular diseases.

Energy-feed power electronic loads can precisely control the phase and magnitude of the power supply output current, achieving the emulation of loads. Moreover, they can feed energy back to the grid for energy regeneration, demonstrating significant research value. This article proposes an energy-fed power electronic load topology and control method that can realize the static and dynamic simulation of linear and non-linear loads and take into account the simulation needs of single-phase, three-phase three-wire, and three-phase four-wire loads. The main circuit uses a two-stage back-to-back AC/DC/AC structure: the front side is a three-phase four-wire split capacitor PWM rectifier bridge, which is used to simulate loads under various operating conditions; the back side is a three-phase three-wire PWM inverter bridge, which realizes the energy feeding back to the grid and reduces the waste of energy; and the intermediate side uses a split capacitor to equalize the voltage and achieve voltage stabilization. The topology is analyzed under the simulation demands of three-phase balanced, three-phase unbalanced, single-phase and non-linear loads. Finally, a MATLAB(R2022a)/Simulink simulation platform is built for a power electronic load with a rated capacity of 200 kVA. The simulation results verify the effectiveness, feasibility, and advancement of the power electronic load proposed in this article.

A parallel active power filter (APF) is generally used to suppress dynamic harmonic current and compensate reactive power in the grid. However, parallel APF may have a negative effect on the load current when compensating the nonlinear load of a voltage source type, which may lead to the amplification effect of the load harmonic current. In this paper, the fundamental causes of harmonic current amplification were analyzed by studying the harmonic current amplification effect when a parallel APF compensates a nonlinear load. According to the results of the theoretical derivation, a feasible method to limit this current amplification effect by changing the system structure and the APF’s own control was proposed, and the corresponding design scheme is given. Finally, the correctness of the theoretical derivation of the harmonic current amplification effect and the feasibility of the proposed solution were proven through simulation and experiment.