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15 results on '"Bindslev, Lene"'

3. Comparison of mesenchymal stromal cells from young healthy donors and patients with severe chronic coronary artery disease

Author

Friis, Tina, Haack-Sørensen, Mandana, Hansen, Susanne Kofoed, Hansen, Louise, Bindslev, Lene, Kastrup, Jens, Friis, Tina, Haack-Sørensen, Mandana, Hansen, Susanne Kofoed, Hansen, Louise, Bindslev, Lene, and Kastrup, Jens

Abstract

It has been questioned whether bone marrow-derived mesenchymal stromal cells (MSCs) from patients with ischemic heart disease are suitable for use in regenerative stem cell therapy. We compared MSCs from patients with chronic coronary artery disease (CAD) and MSCs from young healthy donors with respect to phenotype, proliferation and endothelial differentiation capacity.

Published
2011

4. Mesenchymal stromal cell derived endothelial progenitor treatment in patients with refractory angina

Author

Friis, Tina, Haack-Sørensen, Mandana, Mathiasen, Anders B, Ripa, Rasmus S, Kristoffersen, Ulrik S, Jørgensen, Erik, Hansen, Louise, Bindslev, Lene, Kjær, Andreas, Hesse, Birger, Dickmeiss, Ebbe, Kastrup, Jens, Friis, Tina, Haack-Sørensen, Mandana, Mathiasen, Anders B, Ripa, Rasmus S, Kristoffersen, Ulrik S, Jørgensen, Erik, Hansen, Louise, Bindslev, Lene, Kjær, Andreas, Hesse, Birger, Dickmeiss, Ebbe, and Kastrup, Jens

Abstract

Aims. We evaluated the feasibility, safety and efficacy of intra-myocardial injection of autologous mesenchymal stromal cells derived endothelial progenitor cell (MSC) in patients with stable coronary artery disease (CAD) and refractory angina in this first in man trial. Methods and results. A total of 31 patients with stable CAD, moderate to severe angina and no further revascularization options, were included. Bone marrow MSC were isolated and culture expanded for 6-8 weeks. It was feasible and safe to establish in-hospital culture expansion of autologous MSC and perform intra-myocardial injection of MSC. After six months follow-up myocardial perfusion was unaltered, but the patients increased exercise capacity (p <0.001), reduction in CCS Class (p <0.001), angina attacks (p <0.001) and nitroglycerin consumption (p <0.001), and improved Seattle Angina Questionnaire (SAQ) evaluations (p <0.001). For all parameters there was a tendency towards improved outcome with increasing numbers of cells injected. In the MRI substudy: ejection fraction (p <0.001), systolic wall thickness (p = 0.03) and wall thickening (p = 0.03) all improved. Conclusions. The study demonstrated that it was safe to treat patients with stable CAD with autologous culture expanded MSC. Moreover, MSC treated patients had significant improvement in left ventricular function and exercise capacity, in addition to an improvement in clinical symptoms and SAQ evaluations.

Published
2011

5. Mesenchymal stromal cell derived endothelial progenitor treatment in patients with refractory angina

Author

Friis, Tina, primary, Haack-Sørensen, Mandana, additional, Mathiasen, Anders B., additional, Ripa, Rasmus S., additional, Kristoffersen, Ulrik S., additional, Jørgensen, Erik, additional, Hansen, Louise, additional, Bindslev, Lene, additional, Kjær, Andreas, additional, Hesse, Birger, additional, Dickmeiss, Ebbe, additional, and Kastrup, Jens, additional

Published
2011
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14. <e1>In situ</e1> PCR for detection and identification of fungal species

Author

BINDSLEV, Lene, OLIVER, Richard P., and JOHANSEN, Bo

Abstract

PCR and DNA sequence analysis have become standard tools for identification, detection and phylogenetic analysis of fungi. A large number of species are incapable of growth in the laboratory, making the preparation of pure DNA problematical. The amplification of DNA samples from impure material is subject to misinterpretation if more than one species is present. To overcome this problem, we designed an in situ PCR technique that links PCR amplification to the light microscopic image. The amplified tissue is stained, thus confirming which morphotype has been amplified. The PCR product can then be sequenced. We tested the technique on fixed Blumeria graminis spores and mycelia using primers derived from the sequence of the gene encoding the catalytic subunit of protein kinase A (bka1). This is the first report of in situ PCR on phytopathogenic fungal material. This technique allows positive confirmation of the origin of genes cloned from obligate pathogenic fungi and could be adapted for use on any samples containing mixed fungal species.

Published
2002
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15. Leukotriene D4 induces association of active RhoA with phospholipase C-gamma1 in intestinal epithelial cells.

Author

Thodeti CK, Massoumi R, Bindslev L, and Sjölander A

Subjects
Calcium Signaling drug effects, Calcium Signaling physiology, Cell Line, Epithelial Cells drug effects, Epithelial Cells metabolism, Heterotrimeric GTP-Binding Proteins metabolism, Humans, Intestines drug effects, Microscopy, Confocal, Phospholipase C gamma, Recombinant Fusion Proteins metabolism, GTP-Binding Protein beta Subunits, GTP-Binding Protein gamma Subunits, Intestinal Mucosa metabolism, Isoenzymes metabolism, Leukotriene D4 pharmacology, Type C Phospholipases metabolism, rhoA GTP-Binding Protein metabolism
Abstract

It has been previously suggested that leukotriene-induced Ca2+ signalling is mediated through a Rho-dependent process, but neither direct activation of Rho nor a mechanism underlying such signalling has been reported. Accordingly, we used the Rhotekin binding assay to assess RhoA activation in intestinal epithelial cells and observed that RhoA was activated by leukotriene D4 (LTD4). We also found that, within 15 s, activation of RhoA by LTD4 led to an increased association of RhoA with G-protein betagamma (Gbetagamma) and phospholipase C-gamma1 (PLC-gamma1) in the plasma membrane, as evidenced by the results of co-immunoprecipitation, glutathione S-transferase (GST) pulldown assays, and confocal microscopy. Amounts of RhoA increased in both Gbeta and PLC-gamma1 immunoprecipitates within 15 s of LTD4 treatment. An interaction between RhoA, Gbetagamma and PLC-gamma1 is supported by our finding that a GST fusion protein of constitutively active RhoA (GST-RhoAV14) precipitated Gbetagamma and PLC-gamma1 from cell lysates in an agonist-dependent manner. Such an association is also substantiated by our confocal immunofluorescence results, which revealed that LTD4 induction increased co-localization of constitutively active RhoA and PLC-gamma1 to the plasma membrane of cells transfected with enhanced green fluorescent protein L63RhoA. Furthermore, microinjection of neutralizing RhoA antibodies, but not control antibodies, significantly reduced LTD4-induced Ca2+ mobilization. Our results are the first to demonstrate a LTD4-induced activation of RhoA and more importantly its association with PLC-gamma1, which are essential for the PLC-gamma1-mediated calcium mobilization.

Published
2002
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