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Term small for gestational age (SGA) babies are at risk for developing iron deficiency anemia. The association between maternal and infant iron stores is not clear.To assess proportion of term SGA neonates developing iron deficiency anemia by 10 weeks of age, and measure correlation between iron profile and hepcidin of babies at birth and at 10 weeks of age with maternal iron profile.Prospective cohort study conducted from November, 2018 to April, 2020.120 term SGA babies and their mothers.Hemogram, iron profile and serum hepcidin (every fourth case) estimated in mother, cord blood and baby at 10 weeks. Babies developing anemia at 6 weeks detected by hemogram and ferritin were started on iron supplementation and excluded from the study.Proportion of babies developing iron deficiency anemia at 10 weeks of age.35 (29.2%) of 120 term SGA babies developed anemia(hemoglobin9 g/dL) at 6 weeks. Proportion of infants who developed iron deficiency anemia (hemoglobin9 g/dL and serum ferritin40 μ/dL) at 6 and 10 weeks of age was 14.2% and 23.3%, respectively. No significant correlation was found between hemoglobin, iron and hepcidin of the baby in cord blood and at 10 weeks of age with that of mothers. Serum hepcidin in babies at birth (137.5 ng/mL) were higher than maternal values (128 ng/mL).A significant proportion of term SGA infants developed anemia during early infancy, irrespective of maternal iron status.

Background: India has the world’s largest load of cervical malignancy. A lot of it can be attributed to lack of cervical cancer screening awareness among the general population. The Accredited Social Health Activists (ASHA) are grass root workers who have good reach in the remote areas, where health care facilities are lacking. Training these ASHAs may increase the general awareness about cervical cancer screening. The aim of the study was to train ASHA workers via lectures and question answer sessions and assess the training with pre and post tests. Methods: We organized two training programs of 500 ASHA workers in two tertiary care hospitals with aim of improving their knowledge and attitude about cervical cancer screening which will eventually improve their practice of training women in general population. It comprised of five lectures in language they understand, slogans, posters, question answer session etc. A test comprising of 17 questions was conducted before and after session to check their knowledge and attitude. Results: There was an overall improvement of 35% in knowledge of the ASHAs i.e. 48% answers were correct in pre-test and 78% were correct in post-test. Questions were pertaining to symptomatology, risk factors, screening methods, their utility and prerequisites of performing the screening tests, when and how often to repeat. Improvement was seen in all the areas. There was improvement in attitude too and most of them wanted themselves (98%), their relatives (100%) and the woman within their area (98%) to be screened for cancer cervix. Conclusion: It was a small initiative and successful result was obtained after the training session of ASHAs. These grass root workers may act as key link of awareness for cervical cancer prevention. They live in same community as the client and therefore, have better repo and communication as compared to medical personnel. Though, this study showed improved knowledge and motivation in ASHAs yet the impact on general population needs further evaluation.

The prognostic role of endometriosis amongst women with ovarian clear cell carcinoma (OCCC) remains debatable. The aim of this study was to ascertain the effect of endometriosis on the prognosis of OCCC. A retrospective review of the medical records of 94 women diagnosed and treated for OCCC at a tertiary gynaecological cancer centre in the UK, spanning the period 2010–2019. Women were divided into two groups according to the presence of endometriosis. Clinico-pathological characteristics, progression-free survival (PFS) and overall survival (OS) were collated between the two groups. Forty-six cases of endometriosis-free OCCC (Ef-OCCC) were collated with 48 cases of endometriosis-related OCCC (Er-OCCC). There was no significant difference between the two groups regarding age (p-value = 0.2), FIGO stage (p-value = 0.8), residual disease (RD) (p-value = 0.07), adjuvant chemotherapy agent (p-value = 0.4) or chemo-resistance (p-value = 0.9). The presence of endometriosis did not significantly affect either OS or PFS. The median OS in the Ef-OCCC and Er-OCCC was 55.00 (95% CI 32.00–189.00) and 71.00 (95% CI 47.00–97.00; log rank = 1.35, p-value = 0.2) months. The median PFS in the Ef-OCCC and Er-OCCC group was 39.00 (95% CI 19.00–143.00) and 39.00 (95% CI 19.00–62.00; log rank = 0.7, p-value = 0.4) months. Survival differences between the two groups were not significant after stratification analysis for independent prognosticators. Endometriosis was not independently associated with the prognosis of OCCC either in crude analysis or after stratification for stage and RD. Further larger, well-designed prospective studies are warranted to draw firmer conclusions on the intrinsic link between endometriosis and OCCC.

It has been explored by several researchers that the pattern of development of entrepreneurship among different generation depends upon several aspects of economy.It is also highlighted that generation Z has no desire to be controlled by any other person or boss. By being an entrepreneur, generation Z can actually contribute to the cause they believe in and also be a significant factor of the global economy. This generation openly demonstrates their concern for ethics of certain practices within the tech world. Their experience with the pandemic, natural response to the lock downs, quarantine and school/business closure, high unemployment rate has developed a collective consciousness for generation Z and molded their world view towards ethics and entrepreneurship.The paper attempts to study the relationship between the entrepreneurial intent of generation Z with reference to their characteristics and the role played by ethical values in current scenario. Key Words:Entrepreneurship, Generation Z, Entrepreneurial Spirit, Ethics, Entrepreneurial Intent

The emotions felt by an individual at the workplace play a significant role in the performance output of an employee and the organisation at large. The academic world has been trying to understand the impacts of various such emotions on the productivity of employees but most of the studies have focused on positive emotions like engagement, commitment etc. Envy at workplace is one of the negative emotions felt by the employees which has a significant impact on employee‘s performance and overall behaviour as well. The current study is an attempt to understand what induces envy among employees at workplace, the role played by the leaders and organisational climate and the various positive and negative consequences of envy at workplace. The researchers have proposed a theoretical framework for understanding the construct of workplace envy - its antecedents, moderators, and consequences.

Cervical cancer is the fourth most common cancer in women in the world. To eliminate cervical cancer by 2030, the World Health Organization has given the target of 70 per cent coverage of twice lifetime screening. A multitude of screening methods are available, including cytology, human papillomavirus (HPV) DNA testing and visual inspection tests. Precision tests, including molecular and protein biomarkers such as DNA methylation, p16 immunostaining, and HPV mRNA testing help to enhance specificity of the screening. Worldwide HPV DNA testing with or without cytology is used as a screening method of choice, while in resource-poor settings, visual inspection tests are recommended. The major hurdle is a uniform and systematic implementation with a recall method in the population. Besides, controversies still exist regarding strategies to manage HPV-positive women and developing guidelines to screen the vaccinated population.

The current recruitment scenario, which is highly dynamic and challenging, calls for the attention of academics and practitioners alike. Recently, several studies were conducted to understand the possible relationship between employer branding and employee retention (Gilani & Cunningham, 2017; Wilden, Gudergan, & Lings, 2010). In the present study, an attempt has been made to explore the impact of employer branding on employee recruitment and retention. A comprehensive literature review of previous studies on this topic was conducted, in an attempt to understand the influence of employer branding on each aspect of recruitment. For this purpose, concepts from signalling theory, person-organisation fit and social identity were considered. Careful analysis of previous studies reveals that employer branding has an impact on an organisation's ability, not only to recruit, but also to retain, desired employees. Further, the organisations which are able to successfully project their images as potential employers of choice are bound to reap benefits in the long run.

BACKGROUND: To assess the efficacy of the combined maneuvers in evaluation of post-laparoscopic pain. MATERIAL AND METHODS: A randomized controlled study was conducted. Sixty-four women were included in the study and were randomly divided into two groups. Intervention group received combined maneuvers such as intraperitoneal normal saline infusion, pulmonary recruitment maneuver and local bupivacaine instillation at port sites after laparoscopic surgery. Control group received routine care. MAIN OUTCOME MEASURES: Upper abdominal pain, shoulder pain and incision site pain were noted in both groups at 3, 6, 12, 24 and 48 h postoperatively. RESULTS: The median interquartile range (in centiles) of upper abdominal pain score 3, 6 and 12 h postoperatively in the intervention group was 1.0 (0.25–1.0), 1.0 (0.0–1.0) and 0.50 (0.0–1.0), and in the control group, the values were 2.0 (2.0–1.0), 2.0 (2.0–1.0) and 1.0 (0–1.0) at 3, 6 and 12 h, respectively (p

Purpose: Standardization of colposcopic evaluation is important as it is observer dependent.The aim of the study was to compare the strength of association of colposcopic findings by International Federation of Colposcopy and Cervical Pathology (IFCPC) nomenclature and Swede score. Methods: In the prospective study, 150 women aged 30 to 60 years with abnormal screening results underwent colposcopy, the findings were evaluated using both IFCPC and Swede score and biopsy was taken from abnormal areas. Performances by both the methods were calculated taking biopsy as gold standard. Results: Nineteen ≥ CIN2 lesions were detected. The sensitivity, specificity, positive predictive value and negative predictive value for detecting ≥ CIN2 lesion by IFCPC scoring was 63.6%, 96.0%, 78.7%, 91.9%. Using Swede score, sensitivity, specificity, positive predictive value and negative predictive value at cut off of 8 were 42.4, 95%, 96.6% and 81.8% while at cut-off of 5, these were 96%, 88.3%,76.7% and 93.6%. The agreement between IFCPC major lesion and Swede score ≥ 8 for ≥ CIN2 was 0.626 (pConclusion: Swede score is an easier and more objective method for colposcopic evaluation and a score >8 can be applied to the screen and treat programme.

To study the accuracy of frozen section biopsy for endometrial pathology in high-risk women with abnormal uterine bleeding (AUB).A case-control study was conducted between November 2017 to April 2019, a total of 150 women with postmenopausal bleeding, perimenopausal AUB, and high-risk women of age 40 years with AUB were recruited. All women underwent transvaginal sonography and Doppler, based on age-appropriate endometrial thickness cut-offs 80 women then underwent hysteroscopy. Based on hysteroscopy, women suspicious of malignancy were taken as cases (n = 40) and those with benign findings as controls (n = 40). All cases and controls underwent dilatation and curettage (DC) with frozen section (FS) and routine histopathology.The sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), positive likelihood ratio (LR), negative LR, and overall test accuracy of FS were 90.9%, 93.19%, 83.33%, 96.19%, 13.8, 0.1 and 86.25% respectively for diagnosing endometrial hyperplasia and cancer taking histopathology as the gold standard. Correlation between frozen section biopsy and histopathology was highly significant (p 0.001) on DC specimens and the level of agreement was good (K = 0.778).In women suspicious of malignancy on hysteroscopy, frozen section has high accuracy on DC specimen and can be used to diagnose endometrial hyperplasia and cancer in an effort to fast-track investigations and work-up for definitive treatment while awaiting final histopathology.

Introduction: Vasopressin has been used to control blood loss in gynaecological surgeries such as in myomectomy, abdominal hysterectomy etc. Only few studies have demonstrated its efficacy in Vaginal Hysterectomy (VH) in reducing intraoperative blood loss. VH is frequently performed gynaecological surgery in safe lower dose of vasopressin. There is no consensus on dose of infiltration. Aim: To compare submucosal vasopressin vs saline infiltration during VH and assess surgical parameters i.e., intraoperative blood loss, postinfiltration vitals, ease of dissection, use of electrocautery, need of blood transfusion and complications. Materials and Methods: This randomised controlled trial was done in department of Obstetrics and Gynaecology in collaboration with Department of Anaesthesia from November 2017 to April 2019. Low risk women aged

Introduction/Background Circulating tumor DNA found in women with malignancy, enters plasma due to lysis of cells at the interface between the primary tumor and the circulation. The primary objective of this study was to isolate and quantify cell-free DNA (cfDNA) from peripheral blood, analyze p53 mutations and correlate with tumor burden in epithelial ovarian malignancy. Secondary objective was to study the degree of agreement between cfDNA p53 mutations and tissue p53 immunohistochemistry. Methodology This prospective case-control study was carried out over 18 months from November 2018 to April 2020 at a tertiary teaching institution. Considering the exploratory nature of the study, study group (n=20) comprised women with epithelial ovarian malignancy. Control groups were women with borderline tumors (n=10) and benign epithelial ovarian tumors (n=10). 58 women who were treatment naive and admitted for surgery entered the study but only those with a final histopathology of epithelial ovarian tumor (malignant, borderline and benign) were included. Peritoneal carcinomatosis index (PCI), surgical complexity score and cytoreductive score was calculated in women undergoing primary cytoreduction. Plasma samples for cfDNA was collected just before surgery and stored at -20oC. cfDNA was extracted from plasma serum using a DNA isolation kit and quantified with Nanodrop Spectrophotometer. ARMS PCR was used to detect a point mutation in Exon 8, codon 239 of p53 using primer pairs. p53 immunostaining was performed on tissue samples using monoclonal antibody directed against p53. Statistical analysis was done using SPSS version 21. Results In women with malignant ovarian cancer isolated cfDNA was highest (1330 ng/mL) in comparison to those with benign or borderline ovarian tumors (748.5 ng/mL and 448.5 ng/mL, respectively) reaching statistical significance, p=0.023. Quantity of cfDNA also correlated well with the histopathological grade of the tumor and stage of the disease, p Analysis of cfDNA p53 mutation in exon 8 showed that 55% of the women diagnosed with malignant ovarian tumor harboured this mutation (p=0.043). Correlation of tissue p53 with cfDNA p53 mutation was statistically significant, p=0.007. All women with malignant ovarian tumor in whom cfDNA p53 mutation was present at codon 239 of exon 8 stained positive for tissue p53 mutation. Conclusion cfDNA p53 mutation in exon 8 was detected at higher frequency in women with malignant epithelial ovarian cancer. Significant correlation was seen between tissue p53 and cfDNA p53 mutation suggesting that mutational analysis of cfDNA could act as biomarker for the diagnosis of ovarian tumors. Disclosures Shalini Rajaram – No conflict of interest Aanchal Verma - No conflict of interest Rajarshi Kar - No conflict of interest Vinod Arora - No conflict of interest Bindiya Gupta - No conflict of interest Sandhya Jain - No conflict of interest

Introduction/Background The inguino-femoral lymphadenectomy (IFL) and its associated morbidity remains to date a field of contention. Several intra-operative techniques have been advocated to intimidate the IFL-related complications. Despite the improvements in the IFL technique, apprehension towards operative complications still exists. The intra-operative application of collagen-fibrin sealants (CFS) has emerged as a promising intervention to reduce the IFL-related lymphorrhoea. To ascertain the clinical utility of CFS during IFL, we performed a meta-analysis to draw conclusions about their efficacy with the primary objective of reducing the volume and the duration of lymphatic drainage. A secondary objective was to elucidate its effectiveness in reducing other wound complications. Methodology MEDLINE, Scopus and Cochrane Database were searched for relevant references from inception until August 2020 in line with PRISMA guidelines. Randomized controlled studies (RCTs) and observational studies (OSs) comparing the post-operative morbidity after IFL with or without the use of CFS were included. The modified Jadad score and the methodologic index for non-randomized studies were used to evaluate the quality of the included studies. Dichotomous variables were assessed using odds ratio (OR), whilst continuous variables were assessed using the standartised mean difference (SMD). Confidence intervals were set at 95%. The DerSimonian-Laird random-effects model was used due to the expected inter-study heterogeneity. Statistical analysis was performed using the RevMan software version 5.3. The level of statistical significance was set at p-value Results Six RCTs and four OSs encompassing 305 and 221 patients respectively were included. The studies were of moderate quality and characterised by significant clinical heterogeneity. The meta-analysis of RCTs demonstrated that the application of CFS did neither decrease the length of drainage [SDM -0.55 (95% CI -1.34 to 0.23), p=0.17] nor the amount of drained output [SMD 0.46 (95% CI -0.29 to 1.20), p=0.23]. No significant difference was found regarding the incidence of lymphocele(s) formation [OR 0.96 (95% CI 0.56 – 1.65), p=0.88] or other wound complications. The safety profile of CFS was favourable. No severe adverse sequelae were reported. Conclusion Our findings suggest that the use of CFS was not associated with difference in the incidence of IFL-related lymphorrhoea. Their safety profile was favourable. This evidence is constrained by the data available with an inevitable emphasis on short-term outcomes. In view of the lack of clinical equipoise, more high quality RCTs are warranted to draw firmer conclusions. An attempt should be made at standardising outcome measures, which will improve comparability between studies. Disclosures None.

Introduction/Background Women with endometriosis carry an increased risk for ovarian clear cell ovarian carcinoma (OCCC), also referred to as ‘endometriosis-associated ovarian carcinoma’. Considered as the precursor lesion of a subset of OCCC, the prognostic role of endometriosis amongst women with OCCC remains a field of contention. Few studies have evaluated the prognostic significance of the concurrent endometriosis with conflicting results. The aim of this study was to ascertain the effect of endometriosis on the prognosis of OCCC. Methodology This was a retrospective cohort study, from 2000 to 2019. Population-based prospectively collected data on OCCC with or without concurrent endometriosis were retrieved via the Pan-Birmingham Gynaecological Oncology database. Ninety-four women with a primary diagnosis of OCCC have been divided into groups based upon the detection of cancer arising from ovarian endometriosis (n=48, 51.1%) or not (n= 46, 48.9%) according to Samson and Scott criteria. Chi-squared test, t-test, and univariate/multivariate Cox regression were used. Survival curves were plotted via the Kaplan-Meier method, whilst survival differences were examined via the log-rank test for categorical variables or Cox regression for continuous variables. All reported p-values were two-tailed. Statistical significance was set at p-value Results Women with OCCC arising from endometriosis had significantly lower levels of pre-operative CA-125 (434.63 ± 1135.57 Vs 867.30 ± 1609.67, p-value=0.02) and significantly lower incidence of post-operative residual disease (RD) (p-value=0.02). Age, post-menopausal status, FIGO stage and incidence of capsule rupture were not statistically significant. The mean overall survival (OS) and overall progression free survival (PFS) were 86.35 (95% CI 69.47 – 103.22) and 115.97 (95% CI 98.77 – 133.17) months, respectively. The presence of endometriosis did not affect neither the OS (87.99 Vs 75.30, p-value=0.25) nor the PFS (111.13 Vs 117.42, p-value=0.48). In univariate analysis, the FIGO stage II-IV and RD were correlated with poorer OS, whilst capsule rupture (CR) with poorer PFS. In multivariate analysis, FIGO stage [HR=2.86 (95% CI 1.47 – 5.55), p-value=0.002] and RD [HR=2.52 (95% CI 1.28 – 4.94, p-value=0.007) were found independent predictors for OS, whilst CR [HR=0.3 (95% CI 0.11 – 0.82), p-value=0.02] for PFS, respectively. No factors affected OS after stratification by stage. Conclusion In this cohort concurrent endometriosis was not a predictive factor for the survival of OCCC women. Further studies are warranted to ascertain whether OCCC with or without coexisting endometriosis develop via distinct pathogenic pathways. Disclosures Nil to disclose.

Introduction/Background Embryonal rhabdomyosarcoma (RMS) of the cervix is a rare entity, encountered mainly in the first two decades of life. The literature consists mainly of case reports and few small case series, and no standard treatment guidelines are available. As this is a disease of adolescence, fertility preservation in well selected cases is of paramount importance. Methodology We report 3 cases of embryonal RMS of the cervix in adolescents, along with their clinical presentation, histopathological features, diagnosis, management tailored to individual cases. Results To date, two of the patients have been followed for 5 years and the remaining 1 for 2 years. They all remained asymptomatic, with no evidence of recurrent disease on clinical examination and MRI. Conclusion The embryonal type of cervical RMS is the most frequent type in this age group. Cervical RMS have good prognosis as usually they present early due to clinical features of bleeding or a mass protruding through the vagina. It should be considered as differential diagnosis for continuous vaginal bleeding in a teenager and should be investigated appropriately. The patients diagnosed with RMS should be managed by multidisciplinary team at cancer centre for best outcomes. Given the young age at diagnosis, fertility sparing treatment maintaining the oncological safety is of paramount importance and is associated with long term survival. Disclosures Nil to disclose.

Endometrial cancer is increasingly been diagnosed in women less than 40 years who are desirous of fertility preservation. These are usually type 1 well differentiated tumors with good prognosis. Endometrial biopsy and Magnetic resonance imaging are important for treatment palnning. Conservative mangement may be offered in women with grade 1 tumors confined to endometrium, with no lymphovascular space invasion and extrauterine disease. Progestins including Medroxy preogesterone acetate, megesterol acetate are used for treatment. All efforts should be made for conception once histological remmision is achieved.

Background Ovarian cancer is associated with a high relapse rate and is the fifth leading cause of cancer deaths in women. The genetic profile of a tumor is responsible for deciding response to chemotherapeutic agents. In this study, we investigate the relation between survivin and p53 expression and response to chemotherapeutic agents of primary cultures of ovarian cancer cells established from ascitic fluid. Materials and Method Ascitic fluid and Dulbecco’s modified Eagle medium was mixed in equal proportion in culture flasks and incubated to establish primary culture. The cells were treated with different combinations of carboplatin and paclitaxel with and without survivin small interfering RNA transfection. Cell survival was estimated by MTT assay. Survivin and p53 expression was quantified by real-time polymerase chain reaction. Results Out of 19 ascitic fluid samples, 13 primary cultures of ovarian cancer cells were established. The half maximal inhibitory concentration doses of carboplatin (≥70 μg/mL) and paclitaxel (≥18 μg/mL) were high for 10/13 and 5/13 patients, respectively. Survivin messenger RNA expression was significantly downregulated on treatment with carboplatin (100 μg/mL), paclitaxel (12.5 μg/mL), and a combination of carboplatin (50 μg/mL) and paclitaxel (6.25 μg/mL). Only paclitaxel-treated ovarian cancer cells showed decrease in expression of p53. Survivin small interfering RNA increased sensitivity of the primary cultures to chemotherapeutic agents. Conclusions The present study highlights the fact that establishing primary cultures from ascitic fluid may help to develop personalized treatment regime for individual patients based on their molecular profile. Our study also shows that supplementing taxols drugs with survivin inhibitors may prove to be beneficial in the treatment of ovarian cancer patients.

H1N1 is a novel influenza A strain. Although it is more common in younger population who mostly observe a mild flu, it can cause serious illness in extreme of ages in less than 5 and more than 65-year-old people and in pregnant women. Understanding the effect of viral infection on pregnancy and also the effect of vaccination is essential for improving health outcomes for mother and baby. Antiviral therapy and vaccination are safe during pregnancy.

Objectives This study was designed to correlate expression of epithelial mesenchymal transition (EMT) pathway markers i.e., E-cadherin and Vimentin with surgicopathological extent of EOC and to type the tumour using p53 immunohistochemistry staining. Methods Women with malignant and benign epithelial ovarian tumours were studied. Sample size was calculated with 80% power and 5% level of significance ;22 cases (EOC) and 22 controls (benign ovarian tumour) were recruited. m-RNA and protein expression of E-cadherin and vimentin was done by real time PCR and IHC staining and p53 by IHC. Peritoneal extent of disease was calculated by peritoneal carcinomatosis index (PCI) and tumour resection by completeness of cytoreductive score (CCS) and correlations derived. Results In advanced EOC, positive correlation was found between PCI and CCS with correlation coefficient of 0.495, p-value Conclusions Expression of E-cadherin decreased and Vimentin increased in EOC which is in synchrony with EMT pathway, however larger studies are needed to derive an association between these markers and extent of disease.

Primary pelvic lymphoma is an uncommon tumor. An isolated adnexal or pelvic mass seldom raises the suspicion of lymphoma. They can mimic cancer of ovary, endometrium, cervix or sarcoma. Diagnosis is very important as management changes completely. We report a case of pelvic Non-Hodgkin’s lymphoma masquerading as an adnexal mass and posing dilemma in diagnosis. Diagnosis was made on biopsy. She received 6 cycles of chemotherapy. Unfortunately, she developed recurrence after 1 year.

To test the clinical utility of biomarkers p16/Ki-67 expression in cervical cytology smears as a marker for transforming HPV infection. Experimental study at a tertiary care hospital. Women who were screened positive on Pap and visual inspection tests (n = 280) underwent colposcopy and biopsy. p16/Ki-67 immunostaining was performed in abnormal Pap smears (n = 86), and HPV DNA testing was also performed in the same women. Sensitivity, specificity, negative predictive value, positive predictive value and likelihood ratios were calculated for each biomarker separately and in combination. McNemar test and receiver operating characteristic (ROC) curves were used to compare sensitivity and specificity of biomarkers with HPV DNA. Areas under the ROC curve (AUC) were compared using the Chi-square test. Eighty-six women with abnormal cytology were evaluated with p16/Ki-67 immunocytochemistry; 20.9% (n = 18) and 18.6% (n = 16) were positive for each biomarker, while dual marker was positive in 15% (n = 13). In all smears, the sensitivity of p16INK4a/Ki-67 in detecting CIN 2+ lesion was 76.9% and specificity was 95.8%. For ASCUS (n = 42) and LSIL (n = 23) smears, specificity and negative predictive value of p16/Ki-67 for CIN 2+ were 100% with a likelihood ratio (LR+) of 27 and 25, respectively, suggesting good diagnostic accuracy. In comparison with HPV DNA testing, combined marker p16/Ki-67 was significantly more specific (p = 0.003); AUC was 0.734 and 0.635, respectively. p16/Ki-67 evaluation in cervical cytology is a valuable biomarker in triaging for CIN 2+ disease in ASCUS and LSIL smears.