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3 results on '"Bin-tong Xia"'

1. Single-Cell Transcriptomics-Based Study of Transcriptional Regulatory Features in the Non-Obstructive Azoospermia Testis

Author

Xiao-juan Tang, Qiao-hong Xiao, Xue-lin Wang, Yan He, Ya-nan Tian, Bin-tong Xia, Yang Guo, Jiao-long Huang, Peng Duan, and Yan Tan

Subjects
non-obstructive azoospermia, spermatogenesis, leydig cells, testicular macrophages, transcription factors, Genetics, QH426-470
Abstract

Non-obstructive azoospermia (NOA) is one of the most important causes of male infertility. Although many congenital factors have been identified, the aetiology in the majority of idiopathic NOA (iNOA) cases remains unknown. Herein, using single-cell RNA-Seq data sets (GSE149512) from the Gene Expression Omnibus (GEO) database, we constructed transcriptional regulatory networks (TRNs) to explain the mutual regulatory relationship and the causal relationship between transcription factors (TFs). We defined 10 testicular cell types by their marker genes and found that the proportion of Leydig cells (LCs) and macrophages (tMΦ) was significantly increased in iNOA testis. We identified specific TFs including LHX9, KLF8, KLF4, ARID5B and RXRG in iNOA LCs. In addition, we found specific TFs in iNOA tMΦ such as POU2F2, SPIB IRF5, CEBPA, ELK4 and KLF6. All these identified TFs are strongly engaged in cellular fate, function and homeostasis of the microenvironment. Changes in the activity of the above-mentioned TFs might affect the function of LCs and tMΦ and ultimately cause spermatogenesis failure. This study illustrate that these TFs play important regulatory roles in the occurrence and development of NOA.

Published
2022
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2. [Effects of rapamycin on glucose-induced autophagy and apoptosis of corpus cavernosum smooth muscle cells in SD rats]

Author

Kai-Huai, Ma, You-Wang, Zhang, Long-Rui, Pan, Bin-Tong, Xia, Yang, Guo, Yan, Ding, and Yan, Tan

Subjects
Male, Rats, Sprague-Dawley, Sirolimus, Glucose, Myocytes, Smooth Muscle, Autophagy, Animals, Apoptosis, Rats
Abstract

To observe the effects of rapamycin on glucose-induced autophagy and apoptosis of corpus cavernosum smooth muscle cells (CCSMC) in SD rats.After primary isolation, purification and identification, CCSMCs were treated with glucose at 5.6, 10, 20, 30 and 40 mmol/L for 48 hours. The autophagy flow was detected in the cells transfected with mCherry-GFP-LC3B double fluorescent adenovirus, the protein and mRNA expressions of autophagy-related gene 5 (ATG5), microtubule-associated protein 1 light chain 3 (LC3), P62 and Beclin-1 determined by Western blot and PCR and the apoptosis of the CCSMC measured by flow cytometry, followed by detection of the changes in the protein and mRNA levels of LC3, Beclin-1 and P62 and the apoptosis of the cells after 2-hour pretreatment with 600 nmol/L rapamycin and then 48-hour treatment with 40 mmol/L glucose.Autophagy was observed in the CCSMCs treated with different concentrations of glucose, the strongest in the 10 mmol/L group (P0.05) and then gradually decreasing with the increase of glucose concentration (P0.05). The apoptosis rate of the CCSMCs was remarkably increased in a concentration-dependent manner after treated with glucose (P0.01) though with no statistically significant difference between the 5.6 and 10 mmol/L groups (P = 0.302). After pretreatment with rapamycin, the expression of the LC3 protein and those of the Beclin-1 protein and mRNA were markedly up-regulated (P0.05), while those of the P62 protein and mRNA remarkably down-regulated (P0.05), indicating the enhancement of autophagy, and the apoptosis rate of the CCSMCs dramatically decreased (P0.01).In vitro culture of CCSMCs with glucose within a certain range of concentration can promote autophagy, inhibit the apoptosis and thus have a protective effect on the cells, but when the proper concentration exceeded, it may also reduce autophagy, promote the apoptosis and consequently affect the vitality and function of CCSMCs.

Published
2021

3. Multi- and transgenerational biochemical effects of low-dose exposure to bisphenol A and 4-nonylphenol on testicular interstitial (Leydig) cells

Author

Bin‐Tong Xia, Yan He, Yang Guo, Jiao‐Long Huang, Xiao‐Juan Tang, Jian‐Ru Wang, Yan Tan, and Peng Duan

Subjects
Male, Phenols, Health, Toxicology and Mutagenesis, Animals, Leydig Cells, General Medicine, Management, Monitoring, Policy and Law, Benzhydryl Compounds, Endocrine Disruptors, Toxicology, Rats
Abstract

Bisphenol A (BPA) and 4-nonylphenol (NP) are well-known endocrine-disrupting chemicals (EDCs) that have been proven to affect Leydig cell (LC) functions and testosterone production, but whether BPA and NP have multi- and transgenerational biochemical effects on Leydig cells (LCs) is unknown. Fourier transform infrared (FTIR) spectroscopy is a powerful analytical technique that enables label-free and non-destructive analysis of the tissue specimen. Herein we employed FTIR coupled with chemometrics analysis to identify biomolecular changes in testicular interstitial (Leydig) cells of rats after chronic exposure to low doses of BPA and NP. Cluster segregations between exposed and control groups were observed based on the fingerprint region of 1800-900 cm

Published
2021

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