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Cancer stem cells are pivotal players in tumors initiation, growth, and metastasis. While several markers have been identified, there remain challenges particularly in heterogeneous malignancies like adult soft tissue sarcomas, where conventional markers are inherently overexpressed. Here, we designed BODIPY scaffold fluorescence probes (BD‐IMC‐1, BD‐IMC‐2) that activate via disaggregation targeting for cyclooxygenase (COX), a potential marker for CSCs in sarcoma in clinical pathology. Based on their structures, BD‐IMC‐1 showcased higher susceptibility to disaggregation compared to BD‐IMC‐2, consistent with their selective interaction with COX. Notably, the BD‐IMC‐1 revealed positive cooperativity binding to COX‐2 at sub‐micromolar ranges. Both probes showed significant fluorescence turn‐on upon LPS or PMA triggered COX‐2 upregulation in live RAW264.7, HeLa, and human sarcoma cell line (Saos‐LM2) up to 2‐fold increase with negligible toxicity. More importantly, the BD‐IMC‐1 demonstrated their practical imaging for COX‐2 positive cells in paraffin‐fixed human sarcoma tissue. Considering the fixed tissues are most practiced pathological sample, our finding suggests a potential of disaggregation activated chemosensor for clinical applications. [ABSTRACT FROM AUTHOR]

Disaggregation‐activated turn‐on imaging probes were designed using BODIPY fluorophore and the pan‐COX inhibitor indomethacin, as reported by Woo Young Jang, Jun‐Seok Lee et al. in their Communication (e202405525). The aggregation‐state‐dependent imaging probe demonstrated its practical usages for pan‐COX imaging of fixed clinical tissue from human sarcoma. The cover image was created with BioRender.com...By Kyung Tae Hong; Seung Bin Park; Dhiraj P. Murale; Jung Hoon Lee; Jangsun Hwang; Woo Young Jang and Jun‐Seok LeeReported by Author; Author; Author; Author; Author; Author; Author [Extracted from the article]

Disaggregation‐activated turn‐on imaging probes were designed using BODIPY fluorophore and the pan‐COX inhibitor indomethacin, as reported by Woo Young Jang, Jun‐Seok Lee et al. in their Communication (e202405525). The aggregation‐state‐dependent imaging probe demonstrated its practical usages for pan‐COX imaging of fixed clinical tissue from human sarcoma. The cover image was created with BioRender.com...By Kyung Tae Hong; Seung Bin Park; Dhiraj P. Murale; Jung Hoon Lee; Jangsun Hwang; Woo Young Jang and Jun‐Seok LeeReported by Author; Author; Author; Author; Author; Author; Author [Extracted from the article]

Abstract: Highly active and stable nano-sized Ni catalysts supported on MgO–Al2O3 prepared from hydrotalcite-like materials have been designed for the combined steam and carbon dioxide reforming of methane (CSCRM), which is a useful process to adjust the H2/CO ratio for Fischer–Tropsch process. Ni/MgO–Al2O3 exhibits remarkable coke resistance, while commercial Ni/MgAl2O4 catalyst shows considerable coke deposition during the target reaction. A strong metal to support interaction (SMSI) of Ni/MgO–Al2O3 enhances coke resistance. The change of the surface area and NiO crystallite size with varying the pre-calcination temperature of support was investigated in relation to the coke resistance. It has been concluded that highly dispersed Ni metal pre-calcined at 800°C shows good coke resistance and high activity. As a consequence, Ni/MgO–Al2O3 catalyst will be a promising catalyst in CSCRM for the GTL process. [Copyright &y& Elsevier]