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Stilling basins with sudden expansions are one of the energy dissipation structures. In the hydraulic jump, pressure fluctuations cause significant damages in stilling basins by cavity formation, erosion, and vibration. Roughness can also lead to changes of the behavior of stream lines and vortices. Despite the large number of works on the topic, the role of roughness in spatial hydraulic jumps is not yet fully understood. Present research aimed to study the influence of rough bed on pressure fluctuations of S-jump in abrupt expanding stilling basin. Experiments were conducted in a 0.8 m width and 12 m length flume. Channel expansions ratios were 0.33, 0.5, 0.67, and 1 within the range of Froude numbers, 2 to 9.5. The results showed that roughness decreases intensity of pressure fluctuations in an abrupt expansion stilling basin. Additionally, in sudden expanding sections, the energy loss increases, and the intensity of pressure fluctuations decrease due to the formation of lateral vortices. The reduction rate of maximum pressure fluctuation was 27%, 46%, and 58% for expansion ratio of 0.67, 0.5, and 0.33, respectively. The results revealed the clear dependence of these variables on the Froude number and the distance to the hydraulic jump toe. The maximum values of extreme pressure fluctuations occur in the range 0.609 < X < 3.385, where X is dimensionless distance from the toe of the hydraulic jump, which makes it highly advisable to reinforce the bed of stilling basins in this range. [ABSTRACT FROM AUTHOR]