29 results on '"Biasoli ÉR"'
Search Results
2. Influence of different methods for classification of lymph node metastases on the survival of patients with oral squamous cell carcinoma.
- Author
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Tomo S, de Castro TF, Araújo WAF, Collado FU, Neto SC, Biasoli ÉR, Bernabé DG, and Miyahara GI
- Subjects
- Humans, Lymph Nodes surgery, Lymph Nodes pathology, Squamous Cell Carcinoma of Head and Neck, Neoplasm Staging, Lymphatic Metastasis diagnosis, Lymphatic Metastasis pathology, Retrospective Studies, Carcinoma, Squamous Cell diagnosis, Carcinoma, Squamous Cell surgery, Mouth Neoplasms diagnosis, Mouth Neoplasms surgery, Head and Neck Neoplasms
- Abstract
Background: Despite the advances in the classification of oral squamous cell carcinoma (OSCC) based on its extension by the TNM system, there is still a need for methods to better classify the patients to predict prognosis and indicate adjuvant therapy., Objectives: To analyze the influence of the number of positive lymph nodes (PN), lymph node ratio (LNR), and log odds of positive lymph nodes (LODDS) in survival of patients with OSCC., Methods: Clinicopathologic data from patients with OSCC who were treated with curative purposes by surgery and neck dissection (ND) with or without subsequent adjuvant therapies from 1991 to 2015 was retrospectively assessed. The impact of the PN, LNR, LODDS, and other variables on overall survival (OS) and disease-free survival (DFS) was analyzed in univariate and multivariate analyses., Results: One hundred nineteen patients were included in this study. In the univariate analysis the PN had a significant impact on OS (p = 0.001) and DFS (p = 0.020), and the LNR had a significant impact on the OS (p = 0.042). In the multivariate analysis with other relevant clinicopathologic variables, the PN was the only significantly independent factor influencing in the OS (p = 0.017) but not in DFS (p = 0.096)., Conclusions: The PN is an independent prognostic indicator for OS and DFS in patients with OSCC and has the potential to aggregate the current AJCC classification. The LNR has potential to be an important prognostic indicator, but the methods for this classification require lapidation. The LODDS did not demonstrate prognostic potential., Competing Interests: Declaration of Competing Interest The authors declare that there is no potential conflict of interest regarding this work., (Copyright © 2022. Published by Elsevier Masson SAS.)
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- 2023
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3. Early life stress exacerbates bone resorption and inhibits anxiety-like behaviour induced by apical periodontitis in rats.
- Author
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Bertolini Botelho MC, Cintra LTA, da Silva CC, Mitsuy Kayahara G, Belzunces Pereira R, Oliveira Santos MF, Issamu Miyahara G, Biasoli ÉR, Penha Oliveira SH, and Bernabé DG
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- Animals, Male, Rats, Anxiety, Maternal Deprivation, Rats, Wistar, Stress, Psychological, Alveolar Bone Loss, Bone Resorption, Periapical Periodontitis pathology
- Abstract
Aim: To evaluate the influence of the early life stress (ELS) on the severity of the apical periodontitis (AP) in Wistar rats., Methodology: Forty male Wistar rats were divided into four groups (n = 10): Control rats; AP-rats with AP; ELS-rats subject to ELS; AP + ELS-rats exposed to ELS and subject to AP. ELS was induced by maternal separation (MS) for a period of 3 h for 21 consecutive days. AP was induced via pulp exposure of the first and second right maxillary molars to the oral environment for 40 days. Three days before euthanasia, all rats underwent behavioural analysis to measure anxiety levels by elevated zero maze. Then, the rats were euthanized and the maxillas were removed to assess the occurrence and severity of AP. The periapical region was evaluated for the intensity of the inflammatory infiltrate and the extent of bone loss. The Mann-Whitney test was performed for nonparametric data, and the Tukey's or Student's t-test was performed for parametric data (p < .05)., Results: The intensity of the inflammatory infiltrate was significantly larger in the AP + ELS group when compared with AP group (p < .05). The AP + ELS group exhibited significantly greater alveolar bone loss, with a periapical lesion size of 103.5 ± 29.88, compared with 72.3 ± 22.28 in the AP group (p < .05). Rats with AP displayed higher anxiety-like behaviour in relation to the control group (p < .05). However, exposure to ELS abolished the AP-induced increased anxiety-like 'behaviour' throughout, since that rats from AP + ELS group attended more the open arms than non-stressed rats with AP (p < .05)., Conclusion: Early life stress is predictive of the severity of AP exacerbating the inflammatory process and increasing periapical bone resorption., (© 2022 British Endodontic Society. Published by John Wiley & Sons Ltd.)
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- 2023
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4. Potential of lymph-node ratio as a prognostic factor for patients with oral squamous cell carcinoma.
- Author
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Tomo S, Araújo WAF, de Castro TF, Neto SC, Collado FU, Biasoli ÉR, Bernabé DG, and Miyahara GI
- Subjects
- Humans, Lymph Node Ratio, Squamous Cell Carcinoma of Head and Neck, Prognosis, Retrospective Studies, Lymphatic Metastasis diagnosis, Lymphatic Metastasis pathology, Lymph Nodes surgery, Lymph Nodes pathology, Neoplasm Staging, Mouth Neoplasms diagnosis, Mouth Neoplasms surgery, Carcinoma, Squamous Cell diagnosis, Carcinoma, Squamous Cell surgery, Head and Neck Neoplasms
- Abstract
Background: To analyze the influence of the lymph node ratio (LNR) in survival of patients with OSCC METHODS: Clinicopathologic data from patients with OSCC who were treated with curative surgery and neck dissection (ND) with or without adjuvant therapies from 1991 to 2015 was retrospectively assessed. The impact of LNR and other variables on overall survival (OS) and disease-free survival (DFS) was analyzed in univariate and multivariate analyses., Results: One hundred nineteen patients were included. In the univariate analysis the LNR had a significant impact on OS (p = 0.01) and DFS (p = 0.01). In the multivariate analysis, the LNR was the only significantly independent factor influencing in the OS (p = 0.03). The adjuvant therapies did not influence on the OS (p = 0.42) and DFS (p = 0.10)., Conclusions: The LNR is an independent prognostic factor in patients with OSCC. The LNR alone is not recommended to indicate the performance of adjuvant therapies., Competing Interests: Declaration of Competing Interest The authors declare that there is no potential conflict of interest regarding this work., (Copyright © 2022 Elsevier Masson SAS. All rights reserved.)
- Published
- 2022
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5. Pleomorphic sarcoma of maxillary sinus: Clinical report of a patient initially diagnosed with denture-induced fibrous hyperplasia.
- Author
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Valente VB, Kayahara GM, Bastos DB, Santos IDS, Xavier-Junior JCC, Biasoli ÉR, Miyahara GI, and Bernabé DG
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- Humans, Female, Aged, Maxillary Sinus diagnostic imaging, Hyperplasia pathology, Dentures, Sarcoma diagnostic imaging, Sarcoma pathology, Soft Tissue Neoplasms pathology, Histiocytoma, Malignant Fibrous diagnosis, Histiocytoma, Malignant Fibrous pathology
- Abstract
Undifferentiated pleomorphic sarcoma is a high-grade soft-tissue malignant tumor that is rare in the head and neck region. A 74-year-old woman displayed a large nodular lesion in the maxillary alveolar mucosa, which was initially identified as denture-related fibrous hyperplasia. Although her prosthodontist has adjusted the maxillary complete denture to relieve pressure on the lesion, it increased in size over time. Computed tomography images of the maxillary sinus showed an extensive destructive lesion. A biopsy was performed, and microscopic examination revealed a poorly differentiated malignancy with numerous spindle cells. Immunohistochemistry reactions were negative for CD45, CD30, CD68, CD34, cytokeratin, S100, desmin, and smooth muscle actin. These findings led to the diagnosis of an undifferentiated pleomorphic sarcoma of the maxillary sinus., (Copyright © 2021 Editorial Council for the Journal of Prosthetic Dentistry. Published by Elsevier Inc. All rights reserved.)
- Published
- 2022
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6. HIV-related plasmablastic lymphoma causing extensive bone destruction in the mandible.
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Kayahara GM, Valente VB, Salzedas LMP, Passador-Santos F, Furuse C, Biasoli ÉR, Miyahara GI, and Bernabé DG
- Subjects
- Adult, Female, Humans, Mandible pathology, Mouth pathology, HIV Infections complications, Lymphoma, Large B-Cell, Diffuse complications, Plasmablastic Lymphoma complications, Plasmablastic Lymphoma diagnosis, Plasmablastic Lymphoma pathology
- Abstract
Plasmablastic lymphoma (PBL) is a rare subtype of large B-cell lymphoma commonly associated with HIV infection. HIV-related PBL has a dismal prognosis. The aggressive clinical course of the disease may lead to the development of rapid-growing swellings, like several benign and malignant conditions. Herein, we reported the case of a 38-year-old woman with a painful swelling in the mandible initially diagnosed as an abscess derived from a tooth extraction. Intraoral examination revealed a painful swelling with reddish, white and purplish areas in the posterior region of the mandible without signs of infection. Imaging exams showed an extensive bone destruction in the left mandibular body. Histopathological examination revealed a high proliferation of plasmacytoid cells with nuclear hyperchromatism. Tumor cells were negative for CD20, and positive for Ki-67, CD138, IgG and lambda chain. The diagnosis of oral PBL was defined and serological test showed positivity for HIV. Eight months after starting treatment, the patient died due to complications of cancer treatment. Lymphoproliferative malignancies related to HIV infection should be included in the differential diagnosis of rapid-growing swellings in the oral cavity., (Copyright © 2022 Elsevier Ltd. All rights reserved.)
- Published
- 2022
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7. Predisposing factors for increased cortisol levels in oral cancer patients.
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Figueira JA, Sarafim-Silva BAM, Gonçalves GM, Aranha LN, Lopes FL, Corrente JE, Biasoli ÉR, Miyahara GI, and Bernabé DG
- Abstract
Cancer patients may have a dysregulation of the hypothalamic-pituitary-adrenal (HPA) axis and abnormal secretion of cortisol. Increased cortisol levels have been associated with worse prognosis in patients with different types of tumors. Although anxiety and depression can trigger an abnormal cortisol secretion, little is known regarding the influence of these emotional disorders on HPA axis dysregulation in cancer patients when evaluating together with demographic, clinicopathological and biobehavioral variables. This cross-sectional study analyzed the pre-treatment plasma cortisol levels of 133 patients with oral squamous cell carcinoma (OSCC) and its association with demographic, clinicopathological, biobehavioral and psychological variables. Plasma cortisol levels were measured by electrochemiluminescence, and anxiety and depression symptoms were assessed using Beck Anxiety Inventory (BAI) and Depression (BDI), respectively. Demographic, clinicopathological and biobehavioral data were collected from patients' medical records. Results from multivariate analysis showed that the occurrence of cancer-induced pain was predictive for higher cortisol levels (OR = 5.388, p = 0.003). Men with OSCC were 4.5 times more likely to have higher plasma cortisol levels than women (OR = 4.472, p = 0.018). The effect of sex on cortisol concentrations was lost in the adjusted model for clinical staging (OR = 2.945, p = 0.116). The absence of chronic alcohol consumption history was a protective factor for highest hormone concentrations in oral cancer patients (OR = 0.104, p = 0.004). Anxiety symptoms measured by BAI as "hands trembling" (OR = 0.192, p = 0.016) and being "nervous" (OR = 0.207, p = 0.0004) were associated with lower cortisol levels. In contrast, the feeling of "fear of losing control" was a risk factor for highest hormone concentrations (OR = 6.508, p = 0.0004). The global score and specific symptoms of depression measured by the BDI were not predictive for plasma hormone levels (p > 0.05). Together, our results show that pain, alcohol consumption and feeling fear are independent factors for increased systemic cortisol levels in patients with oral cancer. Therefore, psychological intervention, as well as control of pain and alcohol consumption, should be considered to prevent the negative effects of cortisol secretion dysregulation in cancer patients., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2022 The Authors.)
- Published
- 2022
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8. Blastoid variant of mantle cell lymphoma in palatine tonsil.
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Valente VB, de Castro TF, Takamiya AS, Callestini R, Xavier-Junior JCC, Cortopassi GM, Neto SC, Collado FU, Biasoli ÉR, Miyahara GI, and Bernabé DG
- Subjects
- Aged, Humans, Lymph Nodes, Male, Lymphoma, Mantle-Cell diagnosis, Lymphoma, Mantle-Cell drug therapy, Palatine Tonsil pathology
- Abstract
Blastoid variant of mantle cell lymphoma (MCL) is an aggressive and extremely rare malignancy. MCL may be diagnosed in lymph nodes and/or extranodal sites exhibiting a poor prognosis. MCL with primary presentation in palatine tonsils has been rarely reported. Herein, we report the case of a 73-year-old man with a painless nodular mass on the right palatine tonsil. A biopsy was performed, and microscopic analysis revealed a neoplasm composed of small to medium sized lymphocytes with finely dispersed chromatin, roundish nucleus and many mitoses. The tumor cells were positive for CD20 (L26), CD5 (4C7), Cyclin D1 (EP12), Bcl2 (124) and Ki-67 (MIB-1; 90%), and negative for Bcl6 (PG-B6p), MUM1 (MUM1p) and CD3 (Polyclonal). These findings led to the diagnosis of blastoid variant of MCL. Diagnostic workup with computed tomography scan excluded other sites of disease. The patient was treated successfully with cyclophosphamide, doxorubicin, vincristine and prednisolone (mini-CHOP regimen). Although the blastoid variant of MCL is rare, it should be included in the differential diagnosis of rapid-growing masses in the palatine tonsil., (Copyright © 2021. Published by Elsevier Ltd.)
- Published
- 2021
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9. Stress hormones promote DNA damage in human oral keratinocytes.
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Valente VB, de Melo Cardoso D, Kayahara GM, Nunes GB, Tjioe KC, Biasoli ÉR, Miyahara GI, Oliveira SHP, Mingoti GZ, and Bernabé DG
- Subjects
- 8-Hydroxy-2'-Deoxyguanosine metabolism, Apoptosis, DNA Breaks drug effects, Epithelial Cells, Histones metabolism, Hormones pharmacology, Humans, Hydrocortisone pharmacology, Keratinocytes drug effects, Nitrosamines chemistry, Nitrosamines pharmacology, Norepinephrine pharmacology, Oxidation-Reduction, Nicotiana chemistry, DNA Damage drug effects, Hormones metabolism, Keratinocytes metabolism, Mouth Mucosa metabolism, Stress, Physiological
- Abstract
Chronic stress increases the systemic levels of stress hormones norepinephrine and cortisol. As well as tobacco-specific carcinogen NNK (4-(methylnitrosamine)-1-(3-pyridyl)-1-butanone), they can induce expressive DNA damage contributing to the cancer development. However, it is unknown whether stress hormones have genotoxic effects in oral keratinocytes. This study investigated the effects of stress hormones on DNA damage in a human oral keratinocyte cell line (NOK-SI). NOK-SI cells stimulated with norepinephrine or cortisol showed higher DNA damage compared to untreated cells. Norepinephrine-induced DNA damage was reversed by pre-treatment with beta-adrenergic blocker propranolol. Cells treated with NNK combined to norepinephrine displayed reduced levels of caspases 3 and 7. Cortisol also reduced the activity of pro-apoptotic enzymes. NNK or norepinephrine promoted single-strand breaks and alkali-label side breaks in the DNA of NOK-SI cells. Pre-treatment of cells with propranolol abolished these effects. Carcinogen NNK in the presence or absence of cortisol also induced DNA damage of these cells. The genotoxic effects of cortisol alone and hormone combined with NNK were blocked partially and totally, respectively, by the glucocorticoid receptor antagonist RU486. DNA damage promoted by NNK or cortisol and carcinogen combined to the hormone led to intracellular γH2AX accumulation. The effects caused by NNK and cortisol were reversed by propranolol and glucocorticoid receptor antagonist RU486, respectively. Propranolol inhibited the oxidation of basis induced by NNK in the presence of DNA-formamidopyrimidine glycosylase. DNA breaks induced by norepinephrine in the presence or absence of NNK resulted in higher 8OHdG cellular levels. This effect was also induced through beta-adrenergic receptors. Together, these findings indicate that stress hormones induce DNA damage of oral keratinocytes and could contribute to oral carcinogenesis., (© 2021. The Author(s).)
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- 2021
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10. In situ melanoma of oral cavity: Diagnosis and treatment of a rare entity.
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Cardoso DM, Bastos DB, Dos Santos DM, Conrado-Neto S, Collado FU, Crivelini MM, Xavier-Júnior JCC, Biasoli ÉR, Miyahara GI, and Bernabé DG
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- Aged, Female, Humans, Melanoma pathology, Melanoma diagnosis, Melanoma therapy, Mouth Mucosa pathology
- Abstract
Oral melanoma is an extremely aggressive and rare tumor. Commonly, oral melanomas are diagnosed as invasive tumors, which considerably reduces the chances of cure. In situ oral melanomas being exceedingly rare, which makes its clinicopathological and prognostic characteristics poorly known. Herein, we report a case of 67-year-old non-white woman with a large black patch on the maxillary alveolar mucosa. A biopsy was made and microscopical analysis revealed moderate atypical junctional melanocytic. Tumor cells were positive for S100 (Polyclonal), Melan-A (Clone A103) and Melanosome (HMB-45). The diagnosis of in situ oral melanoma was made and the patient was treated surgically with partial maxillectomy and rehabilitated with obturator prosthesis. Although extremely rare in situ melanomas should be considered in the differential diagnosis of non-invasive pigmented lesions of the oral mucosa., (Copyright © 2020 Elsevier Ltd. All rights reserved.)
- Published
- 2021
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11. Granulomatous ulceration of the palate.
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de Castro TF, Tomo S, Santos-Silva AR, Bernabé DG, Biasoli ÉR, Crivelini MM, Okamoto AC, and Miyahara GI
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- Humans, Palate, Ulcer
- Published
- 2021
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12. Social isolation stress facilitates chemically induced oral carcinogenesis.
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Verza FA, Valente VB, Oliveira LK, Kayahara GM, Crivelini MM, Furuse C, Biasoli ÉR, Miyahara GI, Oliveira SHP, and Bernabé DG
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- Animals, Cytokines metabolism, Inflammation Mediators metabolism, Male, Neoplasm Proteins metabolism, Rats, Rats, Wistar, 4-Nitroquinoline-1-oxide toxicity, Behavior, Animal, Depression metabolism, Depression pathology, Depression physiopathology, Head and Neck Neoplasms chemically induced, Head and Neck Neoplasms metabolism, Head and Neck Neoplasms pathology, Head and Neck Neoplasms physiopathology, Social Isolation, Squamous Cell Carcinoma of Head and Neck chemically induced, Squamous Cell Carcinoma of Head and Neck metabolism, Squamous Cell Carcinoma of Head and Neck pathology, Squamous Cell Carcinoma of Head and Neck physiopathology, Stress, Psychological metabolism, Stress, Psychological pathology, Stress, Psychological physiopathology
- Abstract
Social isolation has affected a large number of people and may lead to impairment of physical and mental health. Although stress resulting from social isolation may increase cancer progression, its interference on tumorigenesis is poorly known. In this study, we used a preclinical model to evaluate the effects of social isolation stress on chemically induced oral carcinogenesis. Sixty-two 21-day-old male Wistar rats were divided into isolated and grouped groups. After 90 days of age, the rats from both groups underwent oral carcinogenesis with 4-nitroquinoline 1-oxide (4NQO) for 20 weeks. All rats were assessed for depressive-like behavior and euthanized for oral squamous cell carcinoma (OSCC) diagnosis and measurement of inflammatory mediators in the tumor microenvironment. Social isolation stress increased the OSCC occurrence by 20.4% when compared to control. Isolated rats also showed higher tumor volume and cachexia than the grouped rats. Social isolation did not induce changes in the depressive-like behavior after carcinogenic induction. Tumors from stressed rats had increased levels of the inflammatory mediators, TNF-alpha, IL1-beta and MCP-1. The concentrations of TNF-alpha and MCP-1 were significantly increased in the large tumors from isolated animals. Higher tumor levels of TNF-alpha, IL-6, IL1-beta and MCP-1 were positively correlated with OSCC growth. This study provides the first evidence that social isolation stress may facilitate OSCC occurrence and tumor progression, an event accompanied by increased local levels of inflammatory mediators., Competing Interests: The authors have declared that no competing interests exist.
- Published
- 2021
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13. Beta-adrenergic blocker inhibits oral carcinogenesis and reduces tumor invasion.
- Author
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Cecilio HP, Valente VB, Pereira KM, Kayahara GM, Furuse C, Biasoli ÉR, Miyahara GI, Oliveira SHP, and Bernabé DG
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- 4-Nitroquinoline-1-oxide administration & dosage, 4-Nitroquinoline-1-oxide toxicity, Animals, Carcinogenesis chemically induced, Carcinogens administration & dosage, Carcinogens toxicity, Cytokines immunology, Cytokines metabolism, Disease Progression, Humans, Male, Mouth Mucosa drug effects, Mouth Mucosa immunology, Mouth Mucosa pathology, Mouth Neoplasms chemically induced, Mouth Neoplasms pathology, Mouth Neoplasms prevention & control, Neoplasm Invasiveness prevention & control, Neoplasms, Experimental chemically induced, Neoplasms, Experimental pathology, Neoplasms, Experimental prevention & control, Rats, Rats, Wistar, Receptors, Adrenergic, beta metabolism, Signal Transduction drug effects, Squamous Cell Carcinoma of Head and Neck chemically induced, Squamous Cell Carcinoma of Head and Neck pathology, Squamous Cell Carcinoma of Head and Neck prevention & control, Tumor Microenvironment drug effects, Tumor Microenvironment immunology, Adrenergic beta-Antagonists administration & dosage, Carcinogenesis drug effects, Mouth Neoplasms drug therapy, Neoplasms, Experimental drug therapy, Propranolol administration & dosage, Squamous Cell Carcinoma of Head and Neck drug therapy
- Abstract
Purpose: Beta-adrenergic signaling can influence cancer progression and the use of beta blockers as adjuvant drugs in oncologic patients has been suggested. However, the involvement of beta-adrenergic blockers in tumorigenesis is poorly understood. This study investigated the action of beta-adrenergic blocker propranolol on tumor onset using a preclinical model of chemically induced oral cancer., Methods: Thirty-two male Wistar rats were subjected to daily subcutaneous injection of beta-blocker propranolol (10 mg/kg; SubQ), while another 32 rats received only a PBS injection (sham group). One week after starting propranolol treatment, all rats were submitted to chemical induction of oral carcinogenesis with 4-nitroquinoline-1-oxide (4NQO). After 16 weeks, they were assessed for occurrence of oral squamous cell carcinoma (OSCC), in addition to measurement of tumor volume and thickness, and tissue levels of cytokines IL-6, TNF-alpha and IL-10 in the tumor microenvironment., Results: Propranolol treatment reduced the occurrence of OSCC by 31%, 95% CI ( - 127, 216). Beta-adrenergic blocker significantly decreased thickness of OSCC when compared with PBS. Rats treated with propranolol exhibited a lower tumor volume when compared with control rats, but this result did not reach statistical significance. Tumors from propranolol-treated rats exhibited reduced concentrations of pro-inflammatory cytokines IL-6 and TNF-α. There was no difference in the IL-10 levels between tumors from propranolol- and sham-treated rats., Conclusion: Beta-adrenergic signaling may be one of the mechanisms associated with chemically induced oral carcinogenesis.
- Published
- 2020
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14. High p16 INK4a immunoexpression is not HPV dependent in oral leukoplakia.
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Tomo S, Biss SP, Crivelini MM, de Oliveira SHP, Biasoli ÉR, Tjioe KC, Bernabé DG, Villa LL, and Miyahara GI
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- Adult, Aged, Aged, 80 and over, Biomarkers, Tumor, Brazil, Female, Humans, Male, Middle Aged, Young Adult, Carcinoma, Squamous Cell, Cyclin-Dependent Kinase Inhibitor p16 metabolism, Head and Neck Neoplasms, Leukoplakia, Oral metabolism, Papillomavirus Infections
- Abstract
Objective: To evaluate the prevalence of HPV DNA detection in fresh tissue from oral leukoplakia by Linear Array assay, and its correlation with p16
INK4a immunoexpression in the northwest region of the São Paulo state, Brazil., Patients and Methods: Fifty patients diagnosed with oral leukoplakia were included in the study. Sociodemographic, clinicopathologic and lifestyle data, fresh tissue and formalin fixed paraffin embedded (FFPE) tissue samples were collected. The fresh tissue was stored at -80 °C and then submitted to further viral DNA detection by the Linear Array method. Immunohistochemical analysis for the p16INK4a expression was performed., Results: Of the 50 patients included in the study, 62 % were men, and the age ranged from 25 to 82 years. Twenty-three (46 %) were elderly, 46 % were middle-aged adults, and only 12 % were young adults. Most patients were smokers (76 %), 14 % were former smokers, and 10 % were non-smokers. Most patients (56 %) were current drinkers, 22 % were ex-drinkers and 22 % were non-drinkers. Thirty-two percent of the lesions presented some degree of dysplasia. No lesions were positive to HPV by Linear Array detection. Thirty (60 %) OL had p16-low immunoexpression and 20 (40 %) had p16-high immunoexpression., Conclusion: HPV was not identified in the population studied. The high p16INK4a immunoexpression is not dependent on HPV in oral leukoplakia. Broader epidemiological studies are required to clarify the geographic variability in the prevalence of HPV in head and neck squamous cell carcinoma and oral potentially malignant lesions., Competing Interests: Declaration of Competing Interest The authors declare that there is no potential conflict of interest regarding this work., (Copyright © 2020 Elsevier Ltd. All rights reserved.)- Published
- 2020
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15. Diagnosis and Treatment of Oral Venous Malformation in a Child.
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Valente VB, Kayahara GM, Miyahara GI, Biasoli ÉR, and Bernabé DG
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- Angiography, Child, Female, Humans, Injections, Intralesional, Mepivacaine therapeutic use, Oleic Acids therapeutic use, Sclerosing Solutions therapeutic use, Sclerotherapy, Vascular Malformations diagnostic imaging, Vascular Malformations therapy
- Abstract
Venous malformations (VMs) are congenital disorders that constitute about 40% of all vascular anomalies. These lesions do not regress spontaneously and may increase in size during childhood. The case of a 10-year-old girl with an extensive oral VM is reported. Intraoral examination revealed the presence of purplish nodules in the alveolar mucosa and gingiva from anterior maxilla. Doppler ultrasound showed a well-defined hypoechoic image and increased vascularization with low blood flow for the alveolar mucosa lesion. The patient was submitted to intralesional injections of the ethanolamine oleate/mepivacaine sclerosing solution. After four sessions, there was a significant reduction of the lesions. However, the patient abandoned the treatment and the oral VM grew progressively. After 1 year, sclerotherapy was resumed and performed weekly. After 10 session of sclerotherapy, the oral VM totally regressed. The childhood is a critical period for oral VM growth. Doppler ultrasound and sclerotherapy can be effective for the management of extensive lesions in children.
- Published
- 2020
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16. Pineal gland protects against chemically induced oral carcinogenesis and inhibits tumor progression in rats.
- Author
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Kayahara GM, Valente VB, Pereira RB, Lopes FYK, Crivelini MM, Miyahara GI, Biasoli ÉR, Oliveira SHP, and Bernabé DG
- Abstract
Clinical investigations suggest that melatonin suppression and circadian dysfunction may be related to cancer development in shift workers. Studies also show that melatonin suppression after pinealectomy increases cancer incidence in preclinical models. However, no study evaluated the influence of pinealectomy on oral cancer development. In the current study, we investigated the effects of pinealectomy on oral squamous cell carcinoma (OSCC) occurrence and progression in rats. Rats submitted to sham surgery were used as control. Pinealectomy promoted an increase of 140% in OSCC occurrence when compared to sham animals. Tumors from pinealectomized rats displayed a higher volume and thickness than the tumors from sham-operated animals. Pinealectomy induced atrophy of the epithelium adjacent to the oral lesions. Pinealectomized rats showed higher mean number of tumor-associated macrophages and eosinophils in the invasive front of OSCC. In addition, nuclear overexpression of ERK1/2 and p53 was also observed in the front of carcinomas from pinealectomized rats. These results reveal that pineal gland plays a protective role against oral carcinogenesis. The melatonin suppression caused by the pinealectomy might contribute to oral cancer development by acting on ERK1/2 and p53 pathways and regulating tumor inflammation., Competing Interests: CONFLICTS OF INTEREST None.
- Published
- 2020
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17. Benign symmetric lipomatosis in the tongue: an uncommon case.
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Bastos DB, Figueira JA, Furuse C, Biasoli ÉR, Miyahara GI, and Bernabé DG
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- Aged, 80 and over, Biopsy, Diagnosis, Differential, Humans, Lipomatosis diagnosis, Lipomatosis, Multiple Symmetrical diagnosis, Male, Tongue pathology, Lipomatosis pathology, Tongue Neoplasms pathology
- Abstract
Symmetric lipomatosis of the tongue (SLT) is rare and characterized by diffuse growth and unencapsulated lipomas. An 87-year-old man was referred for evaluation of tongue lesions. Intraoral findings showed soft yellowish nodules with a smooth shiny surface diffusely on the lateral border of the tongue, bilaterally. Our clinical diagnosis was multiple tongue lipomas and an incisional biopsy was done. Histopathological examination revealed unencapsulated lobules of mature adipocytes with slight variation in the size and shape, confirming the diagnosis of lipoma. The final diagnosis was SLT. On follow-up at 6 months, the tongue findings were unchanged and no new lesions were observed.
- Published
- 2020
18. Childhood trauma is predictive for clinical staging, alcohol consumption, and emotional symptoms in patients with head and neck cancer.
- Author
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Sarafim-Silva BAM, Duarte GD, Sundefeld MLMM, Biasoli ÉR, Miyahara GI, and Bernabé DG
- Subjects
- Adolescent, Adult, Aged, Aged, 80 and over, Alcoholism epidemiology, Alcoholism psychology, Anxiety diagnosis, Brazil epidemiology, Child, Child Abuse psychology, Child, Preschool, Depression diagnosis, Female, Humans, Infant, Infant, Newborn, Life Change Events, Male, Middle Aged, Neoplasm Staging, Prognosis, Young Adult, Alcohol Drinking epidemiology, Anxiety epidemiology, Child Abuse statistics & numerical data, Depression epidemiology, Emotions, Head and Neck Neoplasms diagnosis, Head and Neck Neoplasms epidemiology, Head and Neck Neoplasms pathology, Head and Neck Neoplasms psychology, Squamous Cell Carcinoma of Head and Neck diagnosis, Squamous Cell Carcinoma of Head and Neck epidemiology, Squamous Cell Carcinoma of Head and Neck pathology, Squamous Cell Carcinoma of Head and Neck psychology
- Abstract
Background: Traumatic events in childhood have been associated with the occurrence of anxiety and depression in adulthood. This relation has been investigated in patients with breast cancer; however, it has been little explored in patients with other types of cancer. The objective of this study was to evaluate the occurrence of childhood trauma in patients with head and neck cancer and its association with clinicopathological variables and anxiety and depression levels., Methods: The study included 110 patients with head and neck squamous cell carcinoma (HNSCC) before they started cancer treatment. Clinicopathological and biobehavioral data were collected from patients' medical records. Anxiety and depression levels were assessed with the Beck Anxiety Inventory and the Beck Depression Inventory, respectively. The Childhood Trauma Questionnaire was used to evaluate the occurrence of traumatic events in childhood., Results: One hundred five patients (95.5%) experienced at least 1 type of childhood trauma. Emotional neglect was the most reported childhood trauma (43.8%), and multiple regression revealed that it was an independent variable for advanced clinical staging (β = 2.15, P = .048) and higher alcohol consumption (β = 2.32, P = .031). Patients with HNSCC who experienced more traumatic events in childhood had an almost 12 times greater chance of increased depression levels during the pretreatment period (β = 11.89; P = .0002). The occurrence of physical child neglect was a predictive factor for increased anxiety levels (β = 4.17, P = 0.029)., Conclusions: Traumatic events in childhood are predictive for advanced clinical staging, alcohol consumption, and emotional symptoms in patients with HNSCC, and they should be considered in clinical and psychological intervention strategies during cancer treatment. Cancer 2018;000:000-000. © 2018 American Cancer Society., (© 2018 American Cancer Society.)
- Published
- 2018
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19. Circulating catecholamines are associated with biobehavioral factors and anxiety symptoms in head and neck cancer patients.
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Bastos DB, Sarafim-Silva BAM, Sundefeld MLMM, Ribeiro AA, Brandão JDP, Biasoli ÉR, Miyahara GI, Casarini DE, and Bernabé DG
- Subjects
- Adult, Aged, Anxiety complications, Anxiety pathology, Epinephrine blood, Female, Humans, Leukoplakia, Oral pathology, Male, Middle Aged, Norepinephrine blood, Squamous Cell Carcinoma of Head and Neck complications, Squamous Cell Carcinoma of Head and Neck pathology, Sympathetic Nervous System pathology, Tremor blood, Tremor physiopathology, Anxiety blood, Catecholamines blood, Leukoplakia, Oral blood, Squamous Cell Carcinoma of Head and Neck blood
- Abstract
Studies have shown that stress-related catecholamines may affect cancer progression. However, little is known about catecholamine secretion profiles in head and neck cancer patients. The present study investigated plasma norepinephrine and epinephrine levels in head and neck squamous cell carcinoma (HNSCC) patients and patients with oral leukoplakia, as well as their association with clinicopathological and biobehavioral variables and anxiety symptoms. A total of 93 patients with HNSCC and 32 patients with oral leukoplakia were included. Plasma norepinephrine and epinephrine levels were measured by high performance liquid chromatography with electrochemical detection (HPLC-ED), and psychological anxiety levels were measured by the Beck Anxiety Inventory (BAI). Plasma norepinephrine and epinephrine concentrations were significantly higher in patients with oral and oropharyngeal squamous cell carcinoma (SCC) compared to non-cancer patients. Oral SCC patients displayed plasma norepinephrine levels about six times higher than oropharyngeal SCC patients, and nine times higher than oral leukoplakia patients (p < .001). Plasma epinephrine levels in oral SCC patients were higher compared to the oropharyngeal SCC (p = .0097) and leukoplakia (p < .0001) patients. Oropharyngeal SCC patients had higher plasma norepinephrine (p = .0382) and epinephrine levels (p = .045) than patients with oral leukoplakia. Multiple regression analyses showed that a history of high alcohol consumption was predictive for reduced plasma norepinephrine levels in the oral SCC group (p < .001). Anxiety symptom of "hand tremor" measured by the BAI was an independent predictor for higher plasma norepinephrine levels in HNSCC patients (β = 157.5, p = .0377), while the "heart pounding/racing" symptom was independently associated with higher plasma epinephrine levels in the oropharyngeal SCC group (β = 15.8, p = .0441). In oral leukoplakia patients, sleep deprivation and worse sleep quality were independent predictors for higher plasma norepinephrine levels, while severe tobacco consumption and higher anxiety levels were factors for higher plasma epinephrine levels. These findings suggest that head and neck cancer patients display sympathetic nervous system hyperactivity, and that changes in circulating catecholamines may be associated with alcohol consumption, as well as withdrawal-related anxiety symptoms., Competing Interests: The authors have declared that no competing interests exist.
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- 2018
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20. Stress hormones concentrations in the normal microenvironment predict risk for chemically induced cancer in rats.
- Author
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Valente VB, Verza FA, Lopes FYK, Ferreira JZ, Dos Santos PSP, Sundefeld MLMM, Biasoli ÉR, Miyahara GI, Soubhia AMP, de Andrade M, de Oliveira SHP, and Bernabé DG
- Subjects
- 4-Nitroquinoline-1-oxide pharmacology, Adrenocorticotropic Hormone, Animals, Biomarkers, Tumor, Brain-Derived Neurotrophic Factor, Carcinogenesis metabolism, Carcinogens, Cellular Microenvironment physiology, Corticosterone, Disease Models, Animal, Male, Neoplasms chemically induced, Neoplasms metabolism, Norepinephrine, Rats, Rats, Wistar, Risk Factors, Tongue Neoplasms chemically induced, Hormones metabolism, Tongue metabolism, Tongue Neoplasms metabolism
- Abstract
Evidence show that stress hormones can influence cancer progression, but its role in carcinogenesis is poorly understood. In this study, we used a new method based on oral carcinogenesis model in rats to test the hypothesis that physiological levels of stress hormones in the normal tissue microenvironment would have significant predictive value for chemically induced cancer occurrence. Male Wistar rats were submitted to a tongue biopsy for measuring not-stress induced levels of norepinephrine, corticosterone, adrenocorticotropic hormone (ACTH) and brain-derived neurotrophic factor (BDNF) in the tissue before carcinogenic induction. Rats were treated with the 4-nitroquinoline-1-oxide (4NQO) chemical carcinogen for twenty weeks and then euthanized for microscopic evaluation of the tongue lesions. Increased pre-carcinogen norepinephrine concentrations and reduced basal corticosterone levels in the normal tissue microenvironment were predictive for oral squamous cell carcinoma (OSCC) occurrence. Likewise, increased pre-carcinogen norepinephrine levels in the normal microenvironment were associated a lower expression of pCDKN2a-p16 in OSCCs. Post-carcinogen levels of corticosterone and BDNF in oral leukoplakia tissues (precursor lesion of OSCC) and post-carcinogen corticosterone concentrations in OSCCs were higher than basal levels in the normal mucosa. Increased norepinephrine concentrations in OSCCs were associated to a greater tumor volume and thickness. Furthermore, higher levels of norepinephrine, ACTH and BDNF in OSCCs were associated to a lesser intensity of the lymphoplasmocytic infiltrate. This study shows that pre-carcinogen stress hormones levels in the normal microenvironment may be predictive for chemically induced cancer in rats. Moreover, chemical carcinogenesis can promote stressor-like effects with hormonal changes in the tissue microenvironment, which may be associated to tumor progression., (Copyright © 2017 Elsevier Ltd. All rights reserved.)
- Published
- 2018
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21. Oral ulcers associated with bone destruction as the primary manifestation of histoplasmosis in an immunocompetent patient.
- Author
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Figueira JA, Camilo Júnior D, Biasoli ÉR, Miyahara GI, and Bernabé DG
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- Antifungal Agents therapeutic use, Biopsy, Histoplasma, Histoplasmosis drug therapy, Humans, Immunocompetence, Itraconazole therapeutic use, Jaw Diseases drug therapy, Male, Middle Aged, Oral Ulcer drug therapy, Radiography, Panoramic, Histoplasmosis microbiology, Jaw Diseases microbiology, Oral Ulcer microbiology
- Published
- 2017
- Full Text
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22. Plasma HPV DNA is detectable in oral leukoplakia patients.
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Ferreira LL, Biasoli ÉR, Bernabé DG, Nunes CM, and Miyahara GI
- Subjects
- Adult, Aged, Biopsy, Case-Control Studies, DNA, Viral blood, DNA, Viral isolation & purification, Female, Humans, Leukoplakia, Oral blood, Leukoplakia, Oral pathology, Male, Middle Aged, Papillomaviridae isolation & purification, Papillomavirus Infections blood, Papillomavirus Infections diagnosis, Polymerase Chain Reaction, Predictive Value of Tests, Saliva virology, DNA, Viral genetics, Human Papillomavirus DNA Tests, Leukoplakia, Oral virology, Papillomaviridae genetics, Papillomavirus Infections virology
- Abstract
Background: Oral leukoplakia is considered a potentially malignant lesion for the development of squamous cell carcinoma, and various risk factors may be related to its development and malignant transformation, including the human papillomavirus (HPV). The aim of this case-control study was to detect the presence of HPV in fresh tissue, plasma and saliva samples obtained from patients with and without oral leukoplakia, and verify the correlation of the presence of DNA of HPV between different sources of materials., Methods: In this study, 32 patients with oral leukoplakia and 24 patients selected in a case-control manner were included. DNA extraction from the samples was performed, and afterwards it was amplified by nested polymerase chain reaction (nPCR) for the detection of HPV (nPCR: MY09-MY11/GP05+-GP06+)., Results: The DNA of HPV was found in 68.75% of the fresh tissue samples; in 50% of plasma, and in 62.5% of saliva samples in the group of patients with leukoplakia; in comparison with 45.8%, 54%, and 45.8%, in the fresh tissue, plasma and saliva samples, respectively, in the control group., Conclusion: Based on the present study, there was no difference in the rate of HPV detection in patients with or without oral leukoplakia. However all sources tested in this study were considered suitable for HPV detection, especially plasma samples, which showed be an important non-invasive source of HPV detection in leukoplakia patients., (Copyright © 2017 Elsevier GmbH. All rights reserved.)
- Published
- 2017
- Full Text
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23. Aggressive radiolucent lesion of the mandible.
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Valente VB, Barreto IS, Furuse C, Biasoli ÉR, Miyahara GI, and Bernabé DG
- Subjects
- Aged, Biopsy, Fine-Needle, Combined Modality Therapy, Diagnosis, Differential, Diagnostic Imaging, Diphosphonates therapeutic use, Female, Humans, Immunohistochemistry, Risk Factors, Thyroidectomy, Mandibular Neoplasms secondary, Mandibular Neoplasms therapy, Thyroid Neoplasms pathology, Thyroid Neoplasms therapy
- Published
- 2016
- Full Text
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24. Lip Cancer: A Clinicopathological Study and Treatment Outcomes in a 25-Year Experience.
- Author
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Biasoli ÉR, Valente VB, Mantovan B, Collado FU, Neto SC, Sundefeld ML, Miyahara GI, and Bernabé DG
- Subjects
- Carcinoma, Squamous Cell epidemiology, Female, Humans, Lip Neoplasms epidemiology, Male, Margins of Excision, Middle Aged, Neoplasm Grading, Neoplasm Recurrence, Local, Neoplasm Staging, Retrospective Studies, Survival Analysis, Treatment Outcome, Carcinoma, Squamous Cell pathology, Carcinoma, Squamous Cell surgery, Lip Neoplasms pathology, Lip Neoplasms surgery
- Abstract
Purpose: There are few clinical studies focusing on treatment outcomes of lip cancer. This study investigated the clinicopathologic variables of a large sample of patients with lip squamous cell carcinoma (LSCC) treated in a reference head and neck cancer center for the past 25 years and analyzed the influence of these variables on treatment outcomes., Materials and Methods: This retrospective cohort study reviewed the clinical records of patients with LSCC. Epidemiologic data were age, gender, ethnicity, type of occupation, tobacco smoking, alcohol consumption, comorbid conditions, and family cancer history. Clinicopathologic features included the lip location of the tumor, TNM classification, clinical staging, histopathologic grade, surgical margin analysis, and treatment modality. Local recurrence, second primary tumor, and survival were the outcome variables. Statistical analysis was performed by χ(2) test, Fisher exact test, and binary logistic regression analysis. Survival analysis was assessed through the Kaplan-Meier curve. Level of statistical significance was set at a P value less than .05 for all tests., Results: In total, 144 patients with LSCC were studied. There were 117 men (81.25%) and 27 women (18.75%) with a mean age of 60.21 years. One hundred thirty-four patients (93.05%) were considered of white ethnicity, and in 57 cases (39.58%), the patients reported an occupation that was related to long-term solar exposure. Most cancers had initial clinical staging of 1 or 2 (84.02%). Microscopically, lesions were predominantly well (43.05%) and moderately (40.96%) differentiated tumors. Clinical staging was related to a specific higher survival rate (P = .0049). One hundred twelve cases (77.78%) underwent surgical treatment and only 6 patients (4.80%) had local recurrence, which was directly associated with compromised surgical margins (P = .0320)., Conclusion: A high success rate in LSCC treatment was observed in this study. Compromised surgical margin was associated with tumor recurrence and is a critical event in lip cancer treatment., (Copyright © 2016 American Association of Oral and Maxillofacial Surgeons. Published by Elsevier Inc. All rights reserved.)
- Published
- 2016
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25. Detection of Epstein-Barr virus in different sources of materials from patients with oral lichen planus: a case-control study.
- Author
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Vieira Rda R, Ferreira LL, Biasoli ÉR, Bernabé DG, Nunes CM, and Miyahara GI
- Subjects
- Adult, Aged, Case-Control Studies, DNA, Viral analysis, Female, Herpesvirus 4, Human, Humans, Male, Middle Aged, Polymerase Chain Reaction, Prevalence, Epstein-Barr Virus Infections epidemiology, Lichen Planus, Oral virology
- Abstract
Aims: To detect the presence of Epstein-Barr virus (EBV) DNA in different sources of materials from a matched group of patients with oral lichen planus (OLP) and a group of people without OLP lesions, and to correlate the presence of virus with epidemiological variables of the groups studied., Methods: Fresh tissue samples, saliva, exfoliated cells and plasma of 24 patients with OLP lesions (cases) and 17 patients without OLP lesions (controls) were collected. EBV was detected by nested PCR., Results: Viral positivity was obtained in 62.5% of tissue samples; in 70.8% of exfoliated cell samples; in 33.3% of blood plasma samples and in 75% of saliva samples in the cases; and in 35.3% of tissue samples; 82.4% of exfoliated cell samples; in 47.1% of blood plasma samples and in 64.7% of saliva samples in the controls. There was a predominance of women in both groups. Variables not atrophic-erosive were most affected by EBV., Conclusions: No relationship between EBV and OLP was found. However, all sources tested in this study were considered suitable for the detection of viruses., (Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/)
- Published
- 2016
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26. Oral squamous cell carcinoma misdiagnosed as a denture-related traumatic ulcer: A clinical report.
- Author
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Valente VB, Takamiya AS, Ferreira LL, Felipini RC, Biasoli ÉR, Miyahara GI, and Bernabé DG
- Subjects
- Aged, Denture, Complete adverse effects, Diagnosis, Differential, Female, Humans, Stomatitis, Denture, Ulcer complications, Carcinoma, Squamous Cell diagnosis, Mouth Neoplasms diagnosis, Ulcer diagnosis
- Abstract
A 65-year-old woman presented with an ulcerated lesion in the alveolar ridge mucosa, which appeared after new dentures had been inserted. Despite many treatment attempts, the lesion did not recede, even with the interruption of denture wearing. A biopsy was performed, and histopathologic examination revealed an ulcerated, invasive, poorly differentiated oral squamous cell carcinoma. The time from the patient's first contact with the prosthodontist because of the lesion until the appropriate diagnosis was established was approximately 6 months. This clinical report documents a significant delay in the oral squamous cell carcinoma diagnosis and treatment because of a clinical misdiagnosis of a traumatic ulcer resulting from complete dentures. Prosthodontists should be aware of the importance of early diagnosis of oral cancer among elderly prosthesis wearers., (Copyright © 2016 Editorial Council for the Journal of Prosthetic Dentistry. Published by Elsevier Inc. All rights reserved.)
- Published
- 2016
- Full Text
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27. Total spontaneous regression of a central giant cell granuloma after incisional biopsy: a four-year follow-up case report.
- Author
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Vieira Rda R, Biasoli ÉR, Crivelini MM, and Miyahara GI
- Subjects
- Biopsy methods, Child, Collagen analysis, Diagnosis, Differential, Erythrocytes pathology, Fibrous Dysplasia of Bone diagnosis, Follow-Up Studies, Giant Cells pathology, Humans, Male, Mesoderm pathology, Remission, Spontaneous, Granuloma, Giant Cell diagnosis, Mandibular Diseases diagnosis
- Abstract
Central giant cell granuloma (CGCG) of the jaws represents a localized and benign neoplastic lesion sometimes characterized by aggressive osteolytic proliferation. The World Health Organization defines it as an intraosseous lesion composed of cellular and dense connective tissues that contain multiple hemorrhagic foci, an aggregation of multinucleated giant cells, and occasional bone tissue trabeculae. The origin of this lesion is uncertain; however, factors such as local trauma, inflammation, intraosseous hemorrhage, and genetic abnormalities have been identified as possible causes. CGCG generally affects those younger than 30 years and occurs more frequently in women (2:1). This lesion corresponds to approximately 7% of all benign tumors of the jaws, with prevalence in the anterior region of the jaw. Aggressive lesions are characterized by symptoms, such as pain, numbness, rapid growth, cortical perforation, root resorption, and a high recurrence rate after curettage. In contrast, nonaggressive CGCGs have a slow rate of growth, may contain sparse trabeculation, and are less likely to move teeth or cause root resorption or cortical perforation. Nonaggressive CGCGs are generally asymptomatic lesions and thus are frequently found on routine dental radiographs. Radiographically, the 2 forms of CGCG present as radiolucent, expansive, unilocular or multilocular masses with well-defined margins. The histopathology of CGCG is characterized by multinucleated giant cells, surrounded by round, oval, and spindle-shaped mononuclear cells, scattered in dense connective tissue with hemorrhagic and abundant vascularization foci. The final diagnosis is determined by histopathologic analysis of the biopsy specimen. The preferred treatment for CGCG consists of excisional biopsy, curettage with a safety margin, and partial or total resection of the affected bone. Conservative treatments include local injections of steroids, calcitonin, and antiangiogenic therapy. Drug treatment using antibiotics, painkillers, and corticosteroids and clinical and radiographic monitoring are necessary for approximately 10 days after surgery. There are only a few cases of spontaneous CGCG regression described in the literature; therefore, a detailed case report of CGCG regression in a 12-year-old boy with a 4-year follow-up is presented and compared with previous studies., (Copyright © 2014 American Association of Oral and Maxillofacial Surgeons. Published by Elsevier Inc. All rights reserved.)
- Published
- 2014
- Full Text
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28. Increased plasma and salivary cortisol levels in patients with oral cancer and their association with clinical stage.
- Author
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Bernabé DG, Tamae AC, Miyahara GI, Sundefeld ML, Oliveira SP, and Biasoli ÉR
- Subjects
- Aged, Case-Control Studies, Cross-Sectional Studies, Early Detection of Cancer, Female, Humans, Immunoenzyme Techniques, Male, Middle Aged, Neoplasm Staging methods, Saliva chemistry, Specimen Handling, Biomarkers, Tumor metabolism, Carcinoma, Squamous Cell diagnosis, Hydrocortisone metabolism, Mouth Neoplasms diagnosis, Oropharyngeal Neoplasms diagnosis
- Abstract
Objectives: Dysregulation of the hypothalamus-pituitary-adrenal axis has been observed in patients with cancer. This cross-sectional study investigated whether patients with oral and oropharyngeal squamous cell carcinoma (SCC) show changes in cortisol levels in saliva and plasma compared with three control groups, and evaluated its correlation with clinicopathological data., Methods: Salivary and plasma cortisol levels of 34 patients with oral SCC were compared with hormonal levels of 17 oropharyngeal SCC patients, 17 oral leukoplakia patients, 27 smokers and/or drinkers and 25 healthy volunteers. Multivariate analysis was used to evaluate the impact of clinical variables on the cortisol levels., Results: The plasma (p<0.05) and salivary (p<0.01) cortisol levels were significantly higher in patients with oral SCC compared with all groups. Patients with oropharyngeal SCC had higher levels of salivary cortisol compared with smokers and/or drinkers (p<0.05) and patients with leukoplakia (p<0.01). Patients with advanced-stage oral SCC showed significantly higher levels of cortisol than those in an initial clinical stage. Men with oral SCC had higher salivary cortisol levels than women (p<0.05). Age, smoking, alcohol consumption, presence of teeth and awareness of cancer diagnosis had no significant effect on cortisol levels., Conclusions: These results indicate a dysregulation of cortisol secretion in patients with oral cancer and suggest that this hormone can be a biomarker associated with the disease's clinical status.
- Published
- 2012
- Full Text
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29. Stress hormones increase cell proliferation and regulates interleukin-6 secretion in human oral squamous cell carcinoma cells.
- Author
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Bernabé DG, Tamae AC, Biasoli ÉR, and Oliveira SH
- Subjects
- Analysis of Variance, Cell Line, Tumor, Enzyme-Linked Immunosorbent Assay, Humans, Interleukin-6 genetics, Reverse Transcriptase Polymerase Chain Reaction, Tumor Cells, Cultured, Adrenergic beta-Antagonists pharmacology, Cell Proliferation drug effects, Hydrocortisone pharmacology, Interleukin-6 metabolism, Isoproterenol pharmacology, Norepinephrine pharmacology
- Abstract
Patients with oral cancer can have high psychological distress levels, but the effects of stress-related hormones on oral cancer cells and possible mechanisms underlying these relationships are unknown. In this study, we have investigated the effects of stress-related hormones on interleukin-6 (IL-6) secretion and proliferation of oral squamous cell carcinoma (OSCC) cells. The effects of norepinephrine (NE), and cortisol were studied in SCC9, SCC15, and SCC25 cells and effects of isoproterenol in SCC9 and SCC25 cells. Real-time PCR studies revealed constitutive β1- and β2-adrenergic receptors (β-ARs) expression in the SCC9, SCC15, and SCC25 cells. The results showed that NE and isoproterenol significantly enhanced IL-6 mRNA expression and protein production in supernatants of SCC9 and SCC25 cells. Physiological stress levels of NE and isoproterenol (10 μM) at 1 h elicited the most robust IL-6 increase. Regarding IL-6 secretion, 10 μM NE induced a 5-fold increase at 1 h, 3.7-fold increase at 6 h, and 3.2-fold at 24 h in SCC9 cells. These effects were blocked by the β-adrenergic antagonist propranolol, supporting a role for β-ARs in IL-6 secretion. The effects of cortisol varied according to the hormone concentration. Pharmacological concentrations of cortisol (1000 nM) inhibited IL-6 production by SCC9 and SCC25 cells. Cortisol dose that simulates stress conditions (10 nM) tended to increase IL-6 expression in SCC9 cells. Hormonal doses that simulate stress conditions (10 μM NE, at 6 h in SCC9 and SCC15 cells and 10 nM cortisol, at 48 h in SCC15 cells) stimulated increased cell proliferation. Treatment of SCC9 cells with IL-6 neutralizing ab (10 μg/mL) partially inhibited NE-induced proliferation. Finally, 20 OSCC biopsies were shown to express β1- and β2-ARs. These findings suggest that stress hormones can affect oral cancer cells behavior., (Copyright © 2010 Elsevier Inc. All rights reserved.)
- Published
- 2011
- Full Text
- View/download PDF
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