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13 results on '"Bianca Nascimento de Alcantara"'

1. Dermatite fúngica em paciente canino: Relato de caso

Author

Letícia Samara da Silva Rodrigues, Beatriz dos Santos Fonseca, Bruno de Cássio Veloso de Barros, and Bianca Nascimento de Alcantara

Subjects
hiperpigmentação, malassezia sp., prurido, Veterinary medicine, SF600-1100
Abstract

O presente relato trata-se de um caso de dermatite fúngica causada por Malassezia sp. em uma cadela de nove anos de idade, sem castração, da raça poodle. A cadela foi encaminhada para atendimento clínico com histórico de prurido frequente, lambedura em excesso, forte odor nos canais auditivos, hiperpigmentação na região perianal e perivulvar e pequenas lesões encontradas no corpo com leve alopecia. O tratamento tópico e sistêmico foi solicitado com o intuito de diminuir a colônia fúngica, tratar a otite e propiciar melhor qualidade de vida ao paciente.

Published
2022
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2. Histopathological lesions of congenital Zika syndrome in newborn squirrel monkeys

Author

Bianca Nascimento de Alcantara, Aline Amaral Imbeloni, Darlene de Brito Simith Durans, Marialva Tereza Ferreira de Araújo, Ermelinda do Rosário Moutinho da Cruz, Carlos Alberto Marques de Carvalho, Maria Helena Rodrigues de Mendonça, Jorge Rodrigues de Sousa, Adriana Freitas Moraes, Arnaldo Jorge Martins Filho, Maria de Lourdes Gomes Lima, Orlando Pereira Amador Neto, Jannifer Oliveira Chiang, Sarah Raphaella Rocha de Azevedo Scalercio, Liliane Almeida Carneiro, Juarez Antônio Simões Quaresma, Pedro Fernando da Costa Vasconcelos, and Daniele Barbosa de Almeida Medeiros

Subjects
Medicine, Science
Abstract

Abstract The absence of an adequate animal model for studies has limited the understanding of congenital Zika syndrome (CZS) in humans during the outbreak in America. In this study, we used squirrel monkeys (Saimiri collinsi), a neotropical primate (which mimics the stages of human pregnancy), as a model of Zika virus (ZIKV) infection. Seven pregnant female squirrel monkeys were experimentally infected at three different gestational stages, and we were able reproduce a broad range of clinical manifestations of ZIKV lesions observed in newborn humans. Histopathological and immunohistochemical analyses of early-infected newborns (2/4) revealed damage to various areas of the brain and ZIKV antigens in the cytoplasm of neurons and glial cells, indicative of CZS. The changes caused by ZIKV infection were intrauterine developmental delay, ventriculomegaly, simplified brain gyri, vascular impairment and neuroprogenitor cell dysfunction. Our data show that the ZIKV infection outcome in squirrel monkeys is similar to that in humans, indicating that this model can be used to help answer questions about the effect of ZIKV infection on neuroembryonic development and the morphological changes induced by CZS.

Published
2021
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3. Prenatal disorders and congenital Zika syndrome in squirrel monkeys

Author

Aline Amaral Imbeloni, Bianca Nascimento de Alcantara, Leandro Nassar Coutinho, Sarah Raphaella Rocha de Azevedo Scalercio, Liliane Almeida Carneiro, Karol Guimarães Oliveira, Arnaldo Jorge Martins Filho, Darlene de Brito Simith Durans, Wellington Bandeira da Silva, Bruno Tardelli Diniz Nunes, Livia Medeiros Neves Casseb, Jannifer Oliveira Chiang, Carlos Alberto Marques de Carvalho, Mariana Borges Machado, Juarez Antônio Simões Quaresma, Daniele Barbosa de Almeida Medeiros, and Pedro Fernando da Costa Vasconcelos

Subjects
Medicine, Science
Abstract

Abstract During the Zika virus (ZIKV) outbreak in Brazil (2015–2016), the clinical manifestations associated with its infection were complex and included miscarriage and congenital malformations, not previously described. In this study, we evaluated the prenatal conditions of pregnant female squirrel monkeys (Saimiri collinsi) infected during different gestational thirds (GTs) and assessed all clinical aspects, diagnostic imaging, viremia and the immune response. In our study, 75% of the infected animals in the 1st GT group had significant clinical manifestations, such as miscarriage and prolonged viremia associated with a late immune response. Consequently, their neonates showed fetal neuropathology, such as cerebral hemorrhage, lissencephaly or malformations of the brain grooves, ventriculomegaly, and craniofacial malformations. Thus, our study demonstrated the relevance of pregnant squirrel monkeys as a model for the study of ZIKV infection in neonates due to the broad clinical manifestations presented, including the typical congenital Zika syndrome manifestations described in humans.

Published
2021
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4. Zika Virus Infection Damages the Testes in Pubertal Common Squirrel Monkeys (Saimiri collinsi)

Author

Gabriela da Costa Benchimol, Josye Bianca Santos, Ana Sophia da Costa Lopes, Karol Guimarães Oliveira, Eviny Sayuri Trindade Okada, Bianca Nascimento de Alcantara, Washington Luiz Assunção Pereira, Danuza Leite Leão, Ana Cristina Carneiro Martins, Liliane Almeida Carneiro, Aline Amaral Imbeloni, Sheila Tetsume Makiama, Luiz Paulo Printes Albarelli de Castro, Leandro Nassar Coutinho, Lívia Medeiros Neves Casseb, Pedro Fernando da Costa Vasconcelos, Sheyla Farhayldes Souza Domingues, Daniele Barbosa de Almeida Medeiros, and Sarah Raphaella Rocha de Azevedo Scalercio

Subjects
Zika virus, testes damage, squirrel monkeys, Microbiology, QR1-502
Abstract

During the Zika virus (ZIKV) outbreak and after evidence of its sexual transmission was obtained, concerns arose about the impact of the adverse effects of ZIKV infection on human fertility. In this study, we evaluated the clinical-laboratory aspects and testicular histopathological patterns of pubertal squirrel monkeys (Saimiri collinsi) infected with ZIKV, analyzing the effects at different stages of infection. The susceptibility of S. collinsi to ZIKV infection was confirmed by laboratory tests, which detected viremia (mean 1.63 × 106 RNA copies/µL) and IgM antibody induction. Reduced fecal testosterone levels, severe testicular atrophy and prolonged orchitis were observed throughout the experiment by ultrasound. At 21 dpi, testicular damage associated with ZIKV was confirmed by histopathological and immunohistochemical (IHC) analyses. Tubular retraction, the degeneration and necrosis of somatic and germ cells in the seminiferous tubules, the proliferation of interstitial cells and an inflammatory infiltrate were observed. ZIKV antigen was identified in the same cells where tissue injuries were observed. In conclusion, squirrel monkeys were found to be susceptible to the Asian variant of ZIKV, and this model enabled the identification of multifocal lesions in the seminiferous tubules of the infected group evaluated. These findings may suggest an impact of ZIKV infection on male fertility.

Published
2023
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5. Clinical and serological tests for arboviruses in free-living domestic pigeons (Columba livia)

Author

Bruna Alves Ramos, Jannifer Oliveira Chiang, Lívia Carício Martins, Liliane Leal das Chagas, Franko de Arruda e Silva, Milene Silveira Ferreira, Maria Nazaré Oliveira Freitas, Bianca Nascimento de Alcantara, Sandro Patroca da Silva, Stefânia Araújo Miranda, Barbara Alves Sepulvreda, Layna Thayssa Guimarães Corrêa, Andréa Maria Góes Negrão, Pedro Fernando da Costa Vasconcelos, and Alexandre do Rosário Casseb

Subjects
arbovirus, domestic pigeons, clinical, serological tests, Microbiology, QR1-502, Infectious and parasitic diseases, RC109-216
Abstract

BACKGROUND In this study, we evaluated the role of free-living domestic pigeons (Columba livia) as a reservoir of arboviruses in the city of Belém, state of Pará, Brazil. We investigated the presence of antibodies against the most prevalent arboviruses. OBJECTIVES This study was aimed at evaluating some clinical and physical parameters of domestic pigeons, including the presence of antibodies to Amazon-endemic arboviruses. METHODS Eighty-five healthy pigeons were captured in Mangal das Garças Park, in Belém, and were bled. Upon capture, the birds were subjected to a clinical examination in search of alterations that could indicate the presence of arboviruses. Blood samples were converted to serum and tested using the haemagglutination inhibition (HI) technique with a panel of 19 antigens of arboviruses circulating in the Amazon. The confirmation assay for the positive reactions to the viral species tested by HI was a neutralisation test in new-born Swiss albino mice (Mus musculus) [mouse neutralisation test (MNT)]. FINDINGS A total of 10 (11.8%) serum samples tested positive for antiflavivirus antibodies by HI. All the samples positive for the HI test were subjected to MNT for detection of viruses and yielded negative results (logarithmic neutralisation index < 1.7). MAIN CONCLUSION The results represent the first serological detection of antiarbovirus antibodies in domestic pigeons as potential hosts of arboviruses in Brazil. The detection of haemagglutination-inhibiting antibodies against genus Flavivirus indicated that there was recent contact between the analysed domestic pigeons and these arboviruses. Further studies are needed to evaluate the role of free-living pigeons in the maintenance cycle and spread of arboviruses in the Amazon.

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6. Prenatal disorders and congenital Zika syndrome in squirrel monkeys

Author

Wellington Bandeira da Silva, Livia Medeiros Neves Casseb, Arnaldo Jorge Martins Filho, Karol Guimarães Oliveira, Daniele Barbosa de Almeida Medeiros, Carlos Alberto Marques de Carvalho, Bianca Nascimento de Alcantara, Sarah Raphaella Rocha de Azevedo Scalercio, Leandro Nassar Coutinho, Jannifer Oliveira Chiang, Liliane Almeida Carneiro, Pedro Fernando da Costa Vasconcelos, Bruno Tardelli Diniz Nunes, Aline Amaral Imbeloni, Mariana Borges Machado, Juarez Antônio Simões Quaresma, and Darlene de Brito Simith Durans

Subjects
0301 basic medicine, Prenatal disorders, Pediatrics, medicine.medical_specialty, Science, 030231 tropical medicine, MEDLINE, Diseases, Infec??o por Zika virus / complica??es, Article, 03 medical and health sciences, 0302 clinical medicine, Medical research, Pregnancy, Medicine, Animals, Anormalidades Cong?nitas / veterin?ria, Saimiri, Primatas / anatomia & histologia, Multidisciplinary, business.industry, Zika Virus Infection, Monkey Diseases, Zika Virus, Zika virus / patogenicidade, Fetal Diseases, 030104 developmental biology, Saimiri / anatomia & histologia, Infec??o por Zika virus / veterin?ria, Microcephaly, Female, business, Brazil
Abstract

Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico; Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior; Financiadora de Estudos e Projetos. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Centro Nacional de Primatas. Ananindeua, PA, Brasil / Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Programa de P?s-Gradua??o em Virologia. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Programa de P?s-Gradua??o em Virologia. Ananindeua, PA, Brasil. Rural Federal University of Amazonia. Belem, PA, Brazil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Centro Nacional de Primatas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Centro Nacional de Primatas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Centro Nacional de Primatas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Centro Nacional de Primatas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. University Center of Para. Belem, PA, Brazil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil / University of Par? State. Bel?m, PA, Brazil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Programa de P?s-Gradua??o em Virologia. Ananindeua, PA, Brasil / Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Programa de P?s-Gradua??o em Virologia. Ananindeua, PA, Brasil / Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil / University of Par? State. Bel?m, PA, Brazil. During the Zika virus (ZIKV) outbreak in Brazil (2015?2016), the clinical manifestations associated with its infection were complex and included miscarriage and congenital malformations, not previously described. In this study, we evaluated the prenatal conditions of pregnant female squirrel monkeys (Saimiri collinsi) infected during different gestational thirds (GTs) and assessed all clinical aspects, diagnostic imaging, viremia and the immune response. In our study, 75% of the infected animals in the 1st GT group had significant clinical manifestations, such as miscarriage and prolonged viremia associated with a late immune response. Consequently, their neonates showed fetal neuropathology, such as cerebral hemorrhage, lissencephaly or malformations of the brain grooves, ventriculomegaly, and craniofacial malformations. Thus, our study demonstrated the relevance of pregnant squirrel monkeys as a model for the study of ZIKV infection in neonates due to the broad clinical manifestations presented, including the typical congenital Zika syndrome manifestations described in humans.

Published
2021

7. Histopathological lesions of congenital Zika syndrome in newborn squirrel monkeys

Author

Jannifer Oliveira Chiang, Juarez Antônio Simões Quaresma, Carlos Alberto Marques de Carvalho, Arnaldo Jorge Martins Filho, Marialva Tereza Ferreira de Araújo, Ermelinda do Rosário Moutinho da Cruz, Adriana Freitas Moraes, Liliane Almeida Carneiro, Sarah Raphaella Rocha de Azevedo Scalercio, Aline Amaral Imbeloni, Daniele Barbosa de Almeida Medeiros, Pedro Fernando da Costa Vasconcelos, Maria de Lourdes Gomes de Lima, Darlene de Brito Simith Durans, Orlando Pereira Amador Neto, Bianca Nascimento de Alcantara, Jorge Rodrigues de Sousa, and Maria Helena Rodrigues de Mendonça

Subjects
0301 basic medicine, Pathology, medicine.medical_specialty, Science, 030231 tropical medicine, Exame Neurol?gico / veterin?ria, Diseases, Article, Zika virus, 03 medical and health sciences, Medical research, 0302 clinical medicine, Pregnancy, biology.animal, medicine, Animals, Primate, Pregnancy Complications, Infectious, Saimiri, Primatas / anatomia & histologia, Multidisciplinary, biology, Zika Virus Infection, Brain, Outbreak, Syndrome, Zika Virus, Pregnant female, biology.organism_classification, medicine.disease, Experimental models of disease, 030104 developmental biology, Animals, Newborn, Viral infection, Saimiri / anatomia & histologia, Infec??o por Zika virus / veterin?ria, Medicine, Infectious diseases, Gestation, Immunohistochemistry, C?rebro / les?es, Female, Primatas / les?es, Ventriculomegaly
Abstract

This study was supported by grants from CNPq, CAPES (Zika Fast-Track, 440405/2016-5) and FINEP agencies to P.F.C.V. (CNPq: 303999/2016-0) and D.B.A.M. (CNPq: 306581/2016-7). Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Programa de P?s-Gradua??o em VIrologia. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Centro Nacional de Primatas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil / Par? State University. Bel?m, PA, Brazil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Centro Nacional de Primatas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Centro Nacional de Primatas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil / Par? State University. Bel?m, PA, Brazil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. The absence of an adequate animal model for studies has limited the understanding of congenital Zika syndrome (CZS) in humans during the outbreak in America. In this study, we used squirrel monkeys (Saimiri collinsi), a neotropical primate (which mimics the stages of human pregnancy), as a model of Zika virus (ZIKV) infection. Seven pregnant female squirrel monkeys were experimentally infected at three different gestational stages, and we were able reproduce a broad range of clinical manifestations of ZIKV lesions observed in newborn humans. Histopathological and immunohistochemical analyses of early-infected newborns (2/4) revealed damage to various areas of the brain and ZIKV antigens in the cytoplasm of neurons and glial cells, indicative of CZS. The changes caused by ZIKV infection were intrauterine developmental delay, ventriculomegaly, simplified brain gyri, vascular impairment and neuroprogenitor cell dysfunction. Our data show that the ZIKV infection outcome in squirrel monkeys is similar to that in humans, indicating that this model can be used to help answer questions about the effect of ZIKV infection on neuroembryonic development and the morphological changes induced by CZS.

Published
2021

8. Contributors

Author

Michael J. Adkesson, Patricia Aguilar-Calvo, Bianca Nascimento de Alcantara, Matthew C. Allender, Leonardo Arias-Bernal, Cheryl Asa, Kay A. Backues, James E. Bailey, Karen Bauman, Katherine Belov, Mad Frost Bertelsen, Jocelyn Bezner, Ellen Bronson, Peter Buss, Kenneth Cameron, Michelle Campbell-Ward, Lilian Silva Catenacci, Norin Chai, Sathya K. Chinnadurai, Bruce Christensen, Meredith Martin Clancy, Leigh Clayton, Carmen M.H. Colitz, Galaxia Cortes-Hinojosa, José Luis Crespo-Picazo, Liza Dadone, Marietta Dindo Danforth, Sharon L. Deem, Rosalie Dench, Marion Renée Desmarchelier, Nicola Di Girolamo, Dante Luis Di Nucci, Jessica A. Emerson, Jonathan H. Epstein, Claire Erlacher-Reid, Joseph P. Flanagan, Brett Fundak, Laurie J. Gage, Kathryn C. Gamble, Daniel García-Párraga, Michael M. Garner, Timothy A. Georoff, Kirsten V.K. Gilardi, Martin Gilbert, Steven M. Goodman, Mark Greenberg, Alex David Greenwood, Carsten Grøndahl, Catherine Hadfield, Bálint Halpern, Sarah Hamer, Elizabeth E. Hammond, Robert Harman, Sonia Maria Hernandez, Carolyn Hodo, Erik Hofmeister, Carolyn J. Hogg, Lauren Lynn Howard, Marina Ivančić, Gwen Jankowski, Donald L. Janssen, Carles Juan-Sallés, Kurnia Oktavia Khairani, Matthew E. Kinney, Laura M. Kleinschmidt, Richard Anthony Kock, Corinne P. Kozlowski, Jennifer N. Langan, Alexis Lécu, Gregory A. Lewbart, Kerrie Anne T. Loyd, Imke Lüeders, Khursheed Mama, Christoph Mans, Rachel E. Marschang, Paolo R. Martelli, Gerardo Martinez, Jonna A.K. Mazet, Denise McAloose, Carol U. Meteyer, Michele A. Miller, Ellie Milnes, Christine Molter, Santiago Monsalve, Pete Morkel, Hayley Weston Murphy, Joanne Paul-Murphy, Yvonne Nadler, Julia E. Napier, Pierre Nel, Pauline Nol, Sean O'Sullivan, Francisco Olea-Popelka, Klaus Osterrieder, Annie Page-Karjian, Jean A. Paré, Adriana Pastor, Joost Philippa, Wouter Pieters, Timothy J. Portas, Robin W. Radcliffe, Jan Raines, Bonnie L. Raphael, Fidisoa Rasambainarivo, Patricia Reed, Jack C. Rhyan, Bruce Rideout, John Roberts, Sarah Robinson, Gianmarco Rojas Moreno, Laura Elizabeth Rosen, Elizabeth Marie Rush, David Sanchez-Migallon Guzman, Willem Schaftenaar, Michael R. Schirmacher, Debra A. Schmidt, Kathryn E. Seeley, Michelle E. Shaw, Christina J. Sigurdson, Kurt K. Sladky, Dale Smith, Kristine Smith, Endre Sós, Gerhard Steenkamp, Darrel K. Styles, Hui Suk-Wai, Kathleen E. Sullivan, John M. Sykes, Jessica J. Talbot, Washington Tapia, Karen A. Terio, Scott Terrell, Arshad Haroon Toosy, Dominic A. Travis, Kathryn A. Tuxbury, Eduardo V. Valdes, Caroline Van Hemert, Carrie K. Vance, Michelle L. Verant, Larry Vogelnest, Chris Walzer, Jim Wellehan, Ellen Wiedner, Peregrine L. Wolff, Enrique Yarto-Jaramillo, and Jeffery R. Zuba

Published
2019
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10. In situ immune response and mechanisms of cell damage in central nervous system of fatal cases microcephaly by Zika virus

Author

Eliana Vieira Pinto da Silva, Livia Medeiros Neves Casseb, Pedro Fernando da Costa Vasconcelos, Jannifer Oliveira Chiang, Raimunda do Socorro da Silva Azevedo, Lívia Carício Martins, Sueli Rodrigues, Fernanda Montenegro de Carvalho Araújo, Barbara Cristina Baldez Vasconcelos, Ana Cecília Ribeiro Cruz, Juarez Antônio Simões Quaresma, Beatriz H. Baldez Vasconcelos, Valéria Lima Carvalho, Maria G. L. Queiroz, Bianca Nascimento de Alcantara, Jorge Rodrigues de Sousa, Consuelo Silva de Oliveira, Arnaldo Jorge Martins Filho, and Marialva Tereza Ferreira de Araújo

Subjects
Central Nervous System, Male, 0301 basic medicine, Pathology, Microcephaly, Necrosis, Biopsy, Infec??o pelo Zika virus / complica??es, lcsh:Medicine, Apoptosis, 02 engineering and technology, Brasil / epidemiologia, Zika virus, Infec??o pelo Zika virus / epidemiologia, Morte Perinatal, Perivascular space, lcsh:Science, Multidisciplinary, biology, Zika Virus Infection, Imuno-Histoqu?mica, 021001 nanoscience & nanotechnology, Immunohistochemistry, medicine.anatomical_structure, Cytokines, Female, Meninges / anormalidades, Inflammation Mediators, medicine.symptom, 0210 nano-technology, Encefalopatias / cong?nito, medicine.medical_specialty, Central nervous system, Microcefalia / fisiopatologia, Sistema Nervoso Central / anormalidades, Models, Biological, Article, 03 medical and health sciences, Immune system, medicine, Humans, business.industry, lcsh:R, Immunity, Infant, Newborn, Meninges, Zika Virus, medicine.disease, biology.organism_classification, Zika virus / patogenicidade, 030104 developmental biology, Gliosis, lcsh:Q, Microcefalia / complica??es, Sistema Nervoso Central / patologia, business, Biomarkers
Abstract

Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Secretaria da Sa?de do Estado do Cear?. Laborat?rio Central de Sa?de P?blica. Fortaleza, CE, Brazil. Secretaria de Estado de Sa?de do Rio Grande do Norte. Laborat?rio Central de Sa?de P?blica. Natal, RN, Brazil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil / Universidade do Estado do Par?. Bel?m, PA, Brazil. Universidade Federal do Par?. N?cleo de Medicina Tropical. Bel?m, PA, Brazil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Universidade do Estado do Par?. Bel?m, PA, Brazil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil / Universidade do Estado do Par?. Bel?m, PA, Brazil. Universidade do Estado do Par?. Bel?m, PA, Brazil / Universidade Federal do Par?.N?cleo de Medicina Tropical. Bel?m, PA, Brazil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil / Universidade do Estado do Par?. Bel?m, PA, Brazil. Zika virus (ZIKV) has recently caused a pandemic disease, and many cases of ZIKV infection in pregnant women resulted in abortion, stillbirth, deaths and congenital defects including microcephaly, which now has been proposed as ZIKV congenital syndrome. This study aimed to investigate the in situ immune response profle and mechanisms of neuronal cell damage in fatal Zika microcephaly cases. Brain tissue samples were collected from 15 cases, including 10 microcephalic ZIKV-positive neonates with fatal outcome and fve neonatal control favivirus-negative neonates that died due to other causes, but with preserved central nervous system (CNS) architecture. In microcephaly cases, the histopathological features of the tissue samples were characterized in three CNS areas (meninges, perivascular space, and parenchyma). The changes found were mainly calcifcation, necrosis, neuronophagy, gliosis, microglial nodules, and infammatory infltration of mononuclear cells. The in situ immune response against ZIKV in the CNS of newborns is complex. Despite the predominant expression of Th2 cytokines, other cytokines such as Th1, Th17, Treg, Th9, and Th22 are involved to a lesser extent, but are still likely to participate in the immunopathogenic mechanisms of neural disease in fatal cases of microcephaly caused by ZIKV.

Published
2018
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11. Pharmacognostic analyses and evaluation of the in vitro antimicrobial activity of Acmella oleracea (L.) RK Jansen (Jambu) floral extract and fractions

Author

Ianna Dias Ribeiro da Silva, Bianca Nascimento de Alcantara, Milton Begeres de Almeida, Karla Fabiana Barroso, Yuri Teichi Kobayashi, and Wagner Luiz Ramos Barbosa

Subjects
Pharmacology, Traditional medicine, biology, Chemistry, food and beverages, Pharmaceutical Science, Spilanthol, Plant Science, Pharmacognosy, Antimicrobial, Salmonella typhi, biology.organism_classification, law.invention, chemistry.chemical_compound, Minimum inhibitory concentration, Complementary and alternative medicine, Phytochemical, law, Drug Discovery, Botany, Phytotherapy, Acmella oleracea
Abstract

Acmella oleracea (L) RK Jansen is a typical plant species of Northern Brazil, used in gastronomy and in the Amazonian folk medicine of Para State as analgesic, to treat diseases of the mouth and throat. In industry, extracts of this genus have been used in oral hygiene products and in food compositions, as refreshing and flavoring agent, and is also used in cosmetics and toiletry. This paper reports the pharmacognostic characteristics of the herbal drug (flowers) and the in vitro antimicrobial effect of its ethanol extract (EEFAO) and fractions on pathogenic microorganisms present both in skin and in gastrointestinal tract of domestic animals. EEFAO Hexane, Chloroform, Ethyl Acetate and Methanol fractions at different concentrations (1000, 500, 250, 125, 62.5, 31.25, 15.62, 7.81 mg/mL) were tested against microorganisms (bacteria and fungus). The phytochemical characterization of A. oleracea extract and fractions indicates the presence of, probably, Spilanthol, detected by thin layer chromatography using Dragendorff Reagent. Chloroform fraction inhibited the growth of Salmonella typhi at a Minimum Inhibitory Concentration of 31.25 mg/mL and the microscopic analyses of young flowers demonstrates the presence of undifferentiated hypanthium and involucral bracts, cypselas and vascular bundles, structures also observed in other species of this genus. Quality parameters, including phytochemical description, reported in this work allow the identification and standardization of the flowers as herbal drug, whose microscopic description is very useful because it enables its micrographic characterization. The Chloroform fraction of EEFAO can inhibit the growth of Salmonella typhi making possible the use of A. oleraceae in phytomedicines or conservatives for foods. Key words: Antimicrobial, phytotherapy, flowers pharmacognosy, Salmonella typhi.

Published
2015
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13. Avaliação fitoquímica e potencial cicatrizante do extrato etanólico dos frutos de Jucá (Libidibia ferrea) em ratos Wistar

Author

Yuri Teiichi da Silva Kobayashi, Vívian Tavares de Almeida, Talita Bandeira, Bianca Nascimento de Alcântara, Andressa Santa Brígida da Silva, Wagner Luiz Ramos Barbosa, Paula Barbosa da Silva, Maria Vivina Barros Monteiro, and Milton Begeres de Almeida

Subjects
Pele, Cicatrização, Droga vegetal, Animal culture, SF1-1100
Abstract

Libidibia ferrea é uma planta muito utilizada popularmente para fins terapêuticos, inclusive para acelerar processos de cicatrização de feridas cutâneas. O presente trabalho pesquisou a composição química e avaliou o potencial cicatrizante do extrato etanólico dos frutos de L. ferrea (Mart. ex Tul.) em ratos. Foram utilizados 24 ratos Wistar divididos em quatro grupos. De todos os animais, foi retirado um fragmento de pele do dorso e cada grupo recebeu um tratamento diferente: solução de NaCl 0,9%, digliconato de clorexidina 1%, extrato etanólico dos frutos de Libidibia ferrea 12,5% e 50%. O processo de cicatrização foi avaliado macro e microscopicamente. Para a cicatrização de pele em ratos o extrato etanólico dos frutos de L. ferrea a 12,5% é significativamente mais eficiente do que a 50%. Saponinas, ácidos orgânicos, açúcares redutores, fenóis e taninos, sesquiterpenolactonas e outras lactonas, e antraquinonas foram encontrados no extrato.

Published
2015
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