1. Fatty acid metabolic flux and lipid peroxidation homeostasis maintain the biomembrane stability to improve citrus fruit storage performance
- Author
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Shaowu Xue, Yunliu Zeng, Yunjiang Cheng, Jinqiu Wang, Xiuxin Deng, Haoliang Wan, Rangwei Xu, Juan Xu, Bi Guanglin, Weiwei Wen, Hai Liu, Man Zhu, Wen-Wu Guo, Yizhong He, Hongbin Yang, Zhuoran Li, and Feng-Quan Tan
- Subjects
Linolenic acid ,Glutathione reductase ,Titratable acid ,01 natural sciences ,Analytical Chemistry ,Membrane Potentials ,Polyethylene Glycols ,Lipid peroxidation ,chemistry.chemical_compound ,0404 agricultural biotechnology ,Cell Wall ,Malondialdehyde ,Proline ,Food science ,Plant Proteins ,chemistry.chemical_classification ,Principal Component Analysis ,Superoxide Dismutase ,010401 analytical chemistry ,Fatty Acids ,Fatty acid ,04 agricultural and veterinary sciences ,General Medicine ,040401 food science ,0104 chemical sciences ,Glutathione Reductase ,Phenotype ,chemistry ,Food Storage ,Fruit ,Mutation ,Lipid Peroxidation ,Citrus × sinensis ,Food Science ,Citrus sinensis - Abstract
Little is known about the variations of fresh fruit biomembrane and its physiological and biochemical characteristics during storage. A navel orange mutant 'Gannan No.1' (Citrus sinensis Osbeck) showed higher membrane stability and titratable acid while lower calyx senescence compared with wild-type 'Newhall'. The membrane damage was significantly reduced in 'Gannan No.1' under 10% polyethylene-glycol (41.16% vs. 8.77%) and 30% polyethylene-glycol (52.59% vs.16.11%) treatments on day 45 after harvest. Consistently, membrane electrolyte leakage and malondialdehyde were significantly decreased in 'Gannan No.1', and superoxide dismutase and glutathione reductase were activated. A metabolic analysis was performed to evaluate membrane fatty acid unsaturation and peroxidation. Linolenic acid and hexadecylenic acid contributed to the higher degree of unsaturated fatty acids in 'Gannan No.1'. Furthermore, 'Gannan No.1' accumulated stress-resistant metabolites such as proline, α-tocopherol and glutathione. Correlation analysis of membrane homeostasis indexes with quality parameters showed the importance of biomembrane stability in maintaining citrus fruit quality.
- Published
- 2018