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150 results on '"Bhargava, Prachi"'

5. Computational Study of Conformational Changes in Intrinsically Disordered Regions During Protein-Protein Complex Formation

Author

Kar, Madhabendra Mohon, Bhargava, Prachi, Barik, Amita, Goos, Gerhard, Founding Editor, Hartmanis, Juris, Founding Editor, Bertino, Elisa, Editorial Board Member, Gao, Wen, Editorial Board Member, Steffen, Bernhard, Editorial Board Member, Yung, Moti, Editorial Board Member, Rojas, Ignacio, editor, Valenzuela, Olga, editor, Rojas Ruiz, Fernando, editor, Herrera, Luis Javier, editor, and Ortuño, Francisco, editor

Published
2023
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8. In vitro and in silico evaluation of antibacterial activities of different solvent extracts made from Sisymbrium irio L. seeds.

Author

Tiwari, Madhurima and Bhargava, Prachi

Subjects
*DNA topoisomerase II, *TETRAHYDROFOLATE dehydrogenase, *ARAB medicine, *ANTIBACTERIAL agents, *MOLECULAR docking
Abstract

Sisymbrium irio Linn is one of the underutilized plants, well-known for its traditional importance in Unani Medicine. The present study aimed to evaluate the antibacterial activities of different polarity-based extracts made from seeds of S.irio (Indian variety). The antibacterial activity of 13 different solvent extracts of S.irio seeds was evaluated by the Agar well-diffusion method. Among 13 different solvent seed extracts, ethanolic extract inhibited the growth of all the three bacterial strains used in the study. Further GC-MS analysis of ethanolic extract was done to reveal its phytochemical constituents. Twenty-five different compounds were identified through GC-MS analysis of ethanolic extract of S.irio seeds. Subsequently, for performing in silico antibacterial studies, the identified phytochemicals were first tested for their drug-likeability through Molinspiration software which yielded four compounds. In silico virtual screening via Autodock Vina was done using four phytochemicals against DNA gyrase subunit B and Dihydrofolate reductase. Out of four phytochemical studied through in silico analysis, "Benzene-1,2-dicarboxylicacid, monoamide, N-(1-cyano-1-methylethyl)" was found to inhibit DNA gyrase subunit B most effectively. The present study revealed that Sisymbrium irio displayed potential antibacterial activity and can be used as a good source for designing potent antibacterial agents. [ABSTRACT FROM AUTHOR]

Published
2024
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20. Isolation and screening of keratinolytic bacteria from feather dumping soil near in Lucknow and Kanpur city, North region of Indian.

Author

Latafat, Siddiqui, Mohammed Haris, Ashish, Vimal, Archana, and Bhargava, Prachi

Subjects
CONTAMINATION of poultry, KERATIN, FEATHERS, AGRICULTURAL wastes, PROTEOLYTIC enzymes, SOIL pollution
Abstract

Keratinases are proteolytic enzymes that have the capability of degrading insoluble keratin substrates. LS4, LS5, and KS3 were newly isolated strains showing ability to hydrolyze keratins and keratin based products. In this study, different bacterial strains were isolated from soil, screened for protease and keratinase production, and the best isolates were identified. Serial dilution and plating method were used for isolation of pure bacterial culture. Isolated, 48 strains were screened, and their feather degrading ability on feather meal agar plates and keratin azure-based agar. The proteolytic activity of the isolates was also screened on casein agar. Three strains; LS4, LS5, and KS3 shows keratinolytic activity that reveal the keratinase enzyme on medium; feather meal agar, keratin azure agar, and casein agar. Among all the three strains (LS4, LS5, and KS3), KS3 show largest inhibition zone that show maximum enzymatic activity. For its characterization 16S RNA was performed, sequence KS3 was submitted to GenBank and was identified as Bacillus tropicus KS3. Newly isolated strain B. tropicus KS3 showed the keratinase production and highest keratinase activity (35.06 ± 2.5 U/ml). B. tropicus KS3 also show the highest degradation of feather efficiently showed 84% of whole chicken feather biodegradation at 37 °C within 15 days among all the isolated strains. Keratinase enzyme possesses a wide range of potential applications in the bioremediation of feather waste and bio-fertilizer in agricultural land. The industry demands a better source of this enzyme due to its multivariate application and huge market demand. In the present study, a novel keratinase-producing bacteria was isolated and characterized as Bacillus tropicus KS3 from poultry waste contamination soil. [ABSTRACT FROM AUTHOR]

Published
2024
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23. Virtual screening and molecular dynamics simulation studies to predict the binding of Sisymbrium irio L. derived phytochemicals against Staphylococcus aureus dihydrofolate reductase (DHFR)

Author

Tiwari, Madhurima, Gupta, Sunita, Bhargava, Prachi, Tiwari, Madhurima, Gupta, Sunita, and Bhargava, Prachi

Abstract

The discovery of antibiotics initiated the era of drug innovation and implementation for human and animal health. Very soon, antibiotic resistance started evolving due to over-prescription and heavy usage of drugs leading to deleterious side effects. However, using plant extracts or medicinal plants has emerged as a new approach to dealing with the current problem. One such medicinal plant Sisymbrium irio L. is widely used in Unani therapy as an antimicrobial, analgesic, antipyretic, antioxidant, anti-inflammatory, hepatoprotective, bronchoprotective etc. The phytochemicals extracted from the aerial part of the plant have been used as a natural compound library and screened against a well-known anti-bacterial drug target Dihydrofolate reductase (DHFR) enzyme of Staphylococcus aureus. The top two phytochemicals with lower docking score along with the positive control were subjected to molecular dynamics (MD) simulation studies to examine the stabilities of the complexes over 100 ns, followed by binding free energy estimation. The Root Mean Square Deviation (RMSD), Root Mean Square Fluctuation (RMSF) and Radius of Gyration (Rg) yielded established results throughout the MD run. Moreover, the derived phytochemicals exhibited lower binding free energy values than the positive control that can be tested for its in vitro efficacy, followed by further optimization to attain a potent therapeutic against S. aureus. Taken together, the present study suggests two promising phytochemicals derived from the aerial part of the plant S. irio with stable MD simulation results, strong binding affinity and no side effects.

Published
2022

28. Biological Degradation of Keratin by Microbial Keratinase for Effective Waste Management and Potent Industrial Applications

Author

Latafat, Siddiqui, Mohd. H., Vimal, Archana, and Bhargava, Prachi

Abstract

Enzymes are the biocatalysts synthesized by living organisms having high specificity, catalytic activity, and a broad range of applicability. One such biotechnologically relevant enzyme is keratinase with various industrial applications that capture a significant place in the enzyme market. It belongs to the proteolytic enzyme group that cleaves the highly stable and fibrous protein, keratin through hydrolysis. Keratins are hard-degrading fibrous proteins insoluble in natural solvents and water. It is frequently aggregated in nature and expressively present in the plumes, hair, nail, horn, skins, feet, etc. The broad range of microorganisms, such as bacteria, fungi, and actinomycetes, have been accounted for producing keratinases with significant biotechnological applications. Successful application of this group of enzymes has been seen in various industries such as farming, laundry detergent, cosmetics, animal feed, pharmaceutical, leather, and textile. Moreover, they have found remarkable usability in environment friendly waste management also. This paper focuses on the structure, sources, and various applications of this industrially important enzyme.

Published
2021
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29. Mathematically optimized production, purification and characterization of penicillin G acylase from soil bacterial isolates AA17A and AA17B.

Author

Ajamani, Abhishek, Kumar, Rajesh, Bhargava, Prachi, and Vats, Siddharth

Subjects
PENICILLIN G, SOIL microbiology, ENZYMES industry, BIOLOGICAL mathematical modeling, AMMONIUM sulfate
Abstract

This research article deals with production of industrial enzyme penicillin G acylase from soil bacterial isolates namely AA17A and AA17B, which are selected from 80 soil samples. The strains were selected based on qualitative (turbidity) and quantitative (HPLC) test for 6-aminopenicillanic acid (6APA) production. The enzyme was assayed for its activity and optimized for production of enzyme using design of experiments software (DOE) "Design Expert 8.0.7.1". Optimization of enzyme production of four carbon sources (glucose, glycerol, sucrose and starch), four nitrogen sources (beef extract, tryptone, peptone and yeast extract), for temperature (25°C, 30°C, 35°C and 40°C), four pH (6, 7, 8, 9), four inoculum volumes (2.5 ml, 5.0 ml, 7.5 ml, 10.0 ml) and the phenyl acetic acid (PAA) level (0.15%, 0.17%, 0.185%, 0.2%). The penicillin acylase activity was enhanced to 1.2 fold under following optimized culture conditions: carbon source - glucose (8%), nitrogen source - beef extract (2%), pH 9.0, temperature 30°C, phenyl acetic acid 0.185%, inoculum volume 5 ml. Approximately 1.22-fold purification from the initial culture broth was achieved during ammonium sulphate precipitation (70-80%) with a yield of 4.6% enzyme. The specific activity of the final partially purified enzyme was 13.73 IU/mg protein. [ABSTRACT FROM AUTHOR]

Published
2019

30. Developments in Diagnosis and Antileishmanial Drugs

Author

Bhargava, Prachi and Singh, Rajni

Subjects
Article Subject
Abstract

Leishmaniasis ranks the third in disease burden in disability-adjusted life years caused by neglected tropical diseases and is the second cause of parasite-related deaths after malaria; but for a variety of reasons, it is not receiving the attention that would be justified seeing its importance. Leishmaniasis is a diverse group of clinical syndromes caused by protozoan parasites of the genus Leishmania. It is estimated that 350 million people are at risk in 88 countries, with a global incidence of 1–1.5 million cases of cutaneous and 500,000 cases of visceral leishmaniasis. Improvements in diagnostic methods for early case detection and latest combitorial chemotherapeutic methods have given a new hope for combating this deadly disease. The cell biology of Leishmania and mammalian cells differs considerably and this distinctness extends to the biochemical level. This provides the promise that many of the parasite’s proteins should be sufficiently different from hosts and can be successfully exploited as drug targets. This paper gives a brief overview of recent developments in the diagnosis and approaches in antileishmanial drug discovery and development.

Published
2012
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33. Serological and molecular study of Leptospirain pediatric patients at a tertiary care centre of northern India

Author

Jahan, Asmat, Bhargava, Prachi, Kalyan, Raj Kumar, Verma, Sanjeev Kumar, Gupta, Kamlesh Kumar, Inbaraj, Sophia, and Agarwal, Aman

Abstract

Leptospirosis is considered as one of the most neglected zoonotic tropical diseases. Animals and humans acquire this infection through direct contact with contaminated urine or body fluids of infected animals. This prospective study was undertaken to know the proportion of leptospirosis in pediatric patients presented with acute febrile illness at the Post Graduate Department of Microbiology, King George's Medical University, Lucknow, Uttar Pradesh. Blood samples were collected over a period of one year from January to December 2018 from 70 pediatric patients aged up to 0–12 years presented with febrile illness for 3–14 days. Out of the 70 samples, 7 (10%) samples were found positive by IgM ELISA, 4 (5.7%) samples were positive by MAT and 3(4.3%) were found positive by PCR. Risk factors such as contact with infected animals and contaminated environment were highly associated with seropositivity of leptospirosis. Clinical symptoms such as fever, chills, myalgia, headache, abdominal pain and cough were significantly associated (p < 0.05) with the seropositivity of leptospirosis.

Published
2021
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48. Recent Trends in Organic Farming

Author

Adetunji, Charles Oluwaseun, Anani, Osikemekha Anthony, Olaniyan, Olugbemi T., Inobeme, Abel, Olisaka, Frances N., Uwadiae, Eseosa Oluwadamilare, Obayagbona, Omoregbe Nosa, Soni, Ravindra, editor, Suyal, Deep Chandra, editor, Bhargava, Prachi, editor, and Goel, Reeta, editor

Published
2021
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