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The river Shivna accommodates large scale of agricultural activities, where large quantities of pesticides are applied. To assess the biological impact of pesticide residues that are continuously entering in the water body, biomarkers are common approach in bio-monitoring study as early warning signals to pollutants Shah and Parveen (2022). In the present study analyses of gill histopathological parameters were determined in Channa punctatus. Histopathological biomarkers of toxicity in fish organs are useful indicator of environmental pollution (Bhandare et al, 2019). The fishes Channa punctatus were exposed to lethal concentration at 96 hrs LC50 and sub lethal concentratins at (1/5, 1/10 and 1/15 ppm) of dimethoate for 30 days. [ABSTRACT FROM AUTHOR]