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8. Inhibiting Type VI Secretion System Activity with a Biomimetic Peptide Designed To Target the Baseplate Wedge Complex

Author

Cherrak, Y., Filella-Merce, I., Schmidt, V., Byrne, D., Sgoluppi, V., Chaiaheloudjou, R., Betzi, S., Morelli, X., Nilges, M., Pellarin, R., Durand, E., Laboratoire d'Ingénierie des Systèmes Macromoléculaires, Institut de Microbiologie de la Méditerranée, UMR7255, Aix-Marseille Université-CNRS, Marseille, France., Laboratoire d'ingénierie des systèmes macromoléculaires (LISM), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Bioinformatique structurale - Structural Bioinformatics, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), ED 515 - Complexité du vivant, Sorbonne Université (SU), Institut de Microbiologie de la Méditerranée (IMM), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Centre de Recherche en Cancérologie de Marseille (CRCM), Aix Marseille Université (AMU)-Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'Ingéniérie et Science des Matériaux - EA 4695 (LISM), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS), ANR-17-CE11-0039,T6-PLATFORM,Une approche multidisciplinaire et intégrative pour comprendre l'assemblage, la structure et la dynamique d'une plateforme de queue contractile(2017), ANR-18-CE11-0023,T6MeD-OC,DE LA DYNAMIQUE ET DIVERSITÉ STRUCTURALE DES COMPLEXES MEMBRANES DU T6SS VERS LE DÉVELOPPEMENT D'INHIBITEUR DE LA VIRULENCE(2018), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Aix Marseille Université (AMU), Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris], This work was funded by the Centre National de la Recherche Scientifique, the Aix-Marseille Université, and grants from the Agence Nationale de la Recherche (ANR-18-CE11-0023-01 and ANR-17-CE11-0039) and European Society of Clinical Microbiology and Infectious Diseases (ESCMID) to E.D. E.D. is supported by the Institut National de la Santé et de la Recherche Médicale (INSERM). Y.C. is funded by a doctoral school Ph.D. fellowship from the FRM (ECO20160736014 & FDT201904008052). V.S. is funded by a postdoctoral fellowship from the association Espoir contre la Mucoviscidose., Pellarin, Riccardo, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Paoli-Calmettes, and Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Aix Marseille Université (AMU)

Subjects
bacterial secretion system, bioinformatic, [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM], Virulence Factors, [SDV]Life Sciences [q-bio], virulence inhibitor, type VI secretion system, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, protein-protein interface, Type VI Secretion Systems, Microbiology, QR1-502, [SDV] Life Sciences [q-bio], [SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics, T6SS, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Biomimetics, Escherichia coli, Humans, Peptides, Research Article, biomimetic peptide
Abstract

International audience; Human health is threatened by bacterial infections that are increasingly resistant to multiple drugs. A recently emerged strategy consists of disarming pathogenic bacteria by targeting and blocking their virulence factors. The type VI secretion system (T6SS) is a widespread secretion nanomachine encoded and employed by pathogenic strains to establish their virulence process during host invasion. Given the conservation of T6SS in several human bacterial pathogens, the discovery of an effective broad-spectrum T6SS virulence blocker represents an attractive target for development of antivirulence therapies. Here, we identified and validated a protein-protein interaction interface, TssK-TssG, as a key factor in the assembly of the T6SS baseplate (BP) complex in the pathogen enteroaggregative Escherichia coli (EAEC). In silico and biochemical studies revealed that the determinants of the interface are broadly conserved among pathogenic species, suggesting a role for this interface as a target for T6SS inhibition. Based on the high-resolution structure of the TssKFGE wedge complex, we rationally designed a biomimetic cyclic peptide (BCP) that blocks the assembly of the EAEC BP complex and inhibits the function of T6SS in bacterial cultures. Our BCP is the first compound completely designed from prior structural knowledge with anti-T6SS activity that can be used as a model to target human pathogens. IMPORTANCE New therapeutic options are urgently needed to fight drug-resistant and life-threatening infections. In contrast to antibiotics that inhibit the growth pathways of bacteria, the antivirulence strategy is a promising approach to disarm pathogens by interfering with bacterial virulence factors without exerting evolutionary pressure. The type VI secretion system (T6SS) is used by many pathogens, including members of the antibiotic-resistant ESKAPE bacteria (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp.), to establish their virulence during the invasion of the human host. Although the T6SS is undoubtedly involved in pathogenesis, strategies targeting this virulence factor are crucially lacking. Here, we used a combination of genetics, microbiology, biochemical, biophysics, and bioinformatics approaches to rationally design a biomimetic peptide that interferes with T6SS assembly and functioning. This study represents a novel proof of concept for an antivirulence strategy which aims to interfere with the assembly of the T6SS.

Published
2021

10. Pharmacological inhibition of syntenin PDZ2 domain impairs breast cancer cell activities and exosome loading with syndecan and EpCAM cargo

Author

Leblanc, R., primary, Kashyap, R., additional, Barral, K., additional, Egea‐Jimenez, A.L., additional, Kovalskyy, D., additional, Feracci, M., additional, Garcia, M., additional, Derviaux, C., additional, Betzi, S., additional, Ghossoub, R., additional, Platonov, M., additional, Roche, P., additional, Morelli, X., additional, Hoffer, L., additional, and Zimmermann, Pascale, additional

Published
2020
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13. Crystal structure of dCK mutant C3S

Author

Saez-Ayala, M., primary, Rebuffet, E., additional, Hammam, K., additional, Gros, L., additional, Lopez, S., additional, Hajem, B., additional, Humbert, M., additional, Baudelet, E., additional, Audebert, S., additional, Betzi, S., additional, Lugari, A., additional, Combes, S., additional, Pez, D., additional, Letard, S., additional, Mansfield, C., additional, Moussy, A., additional, de Sepulveda, P., additional, Morelli, X., additional, and Dubreuil, P., additional

Published
2017
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14. Crystal structure of dCK mutant C3S in complex with masitinib and UDP

Author

Rebuffet, E., primary, Hammam, K., additional, Saez-Ayala, M., additional, Gros, L., additional, Lopez, S., additional, Hajem, B., additional, Humbert, M., additional, Baudelet, E., additional, Audebert, S., additional, Betzi, S., additional, Lugari, A., additional, Combes, S., additional, Pez, D., additional, Letard, S., additional, Mansfield, C., additional, Moussy, A., additional, de Sepulveda, P., additional, Morelli, X., additional, and Dubreuil, P., additional

Published
2017
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15. Crystal structure of dCK mutant C3S in complex with imatinib and UDP

Author

Saez-Ayala, M., primary, Rebuffet, E., additional, Hammam, K., additional, Gros, L., additional, Lopez, S., additional, Hajem, B., additional, Humbert, M., additional, Baudelet, E., additional, Audebert, S., additional, Betzi, S., additional, Lugari, A., additional, Combes, S., additional, Pez, D., additional, Letard, S., additional, Mansfield, C., additional, Moussy, A., additional, de Sepulveda, P., additional, Morelli, X., additional, and Dubreuil, P., additional

Published
2017
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19. Accelerating small compounds discovery targeting protein-protein interaction

Author

Milhas, S., Betzi, S., Roche, P., Cholay, M., Restouin, A., Basse, M. -J, Heremans, A., Kashyap, R., Lissitzky, J. -C, Lugari, A., Roux, T., Eydoux, C., Derviaux, C., Badol, M., Hamon, V., Gourdel, M. -E, Combes, S., Brunel, J. -M, Rain, J. -C, Zimmermann, P., Aurrand-Lions, M., Trinquet, E., Yves Collette, Guillemot, J. -C, Morelli, X., Architecture et fonction des macromolécules biologiques (AFMB), Institut National de la Recherche Agronomique (INRA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Bourne, Yves, and Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU)-Institut National de la Recherche Agronomique (INRA)

Subjects
[SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM], [SDV.BIBS] Life Sciences [q-bio]/Quantitative Methods [q-bio.QM], [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM], [SDV.BBM.BS] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM], [SDV.BIBS]Life Sciences [q-bio]/Quantitative Methods [q-bio.QM]
Abstract

40th Congress of the Federation-of-European-Biochemical-Societies (FEBS) - The Biochemical Basis of Life, Berlin, GERMANY, JUL 04-09, 2015; International audience; no abstract

Published
2015

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