35 results on '"Bertoldo, G."'
Search Results
2. Validation of Esaso Classification of Diabetic Maculopathy
- Author
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Panozzo, G., Franzolin, E., Giannarelli, Diana, Dalla Mura, G., Longo, Anita Rosa, Cicinelli, M. V., Angelini, E., Airaghi, P., Avitabile, T., Bandello, F., Beccastrini, A., Benedetti, G., Bertuzzi, Paolo Francesco, Bonfiglio, V. M. E., Boscia, F., Carnevali, A., Carosielli, M., Cereda, M. G., Contardi, C., Coppola, M., Costagliola, C., Cristofolini, R., Cucciniello, P., D'Aloisio, R., De Bernardo, M., De Filippis, Alessandro, Dell'Omo, Roberto, Di Paola, I., Dell'Acqua, Massimiliano, Franco, Alessio, Grassi, M. O., Gregori, G., Gusson, E., Lattanzio, R., Lanzetta, P., Longo, A., Marchini, G., Marolo, P., Mastropasqua, R., Bertoldo, G. M., Monteleone, Giorgio, Ortisi, E., Parisi, Giuseppe, Parrulli, S., Pucci, P., Pastore, M. R., Reibaldi, M., Rizzo, Stanislao, Romano, Federica, Rosa, N., Sarao, V., Scarpa, G., Scorcia, V., Scupola, Andrea, Staurenghi, G., Sunseri Trapani, V., Tognetto, D., Trabucchi, G., Vaccaro, S., Vadala, M., Veritti, D., Vinciguerra, A. L., Zanzottera, E. C., Giannarelli D., Longo R., Bertuzzi F., De Filippis A., Dell'Omo R., Dell'Acqua M., Franco A., Monteleone G., Parisi G., Rizzo S. (ORCID:0000-0001-6302-063X), Romano F., Scupola A., Panozzo, G., Franzolin, E., Giannarelli, Diana, Dalla Mura, G., Longo, Anita Rosa, Cicinelli, M. V., Angelini, E., Airaghi, P., Avitabile, T., Bandello, F., Beccastrini, A., Benedetti, G., Bertuzzi, Paolo Francesco, Bonfiglio, V. M. E., Boscia, F., Carnevali, A., Carosielli, M., Cereda, M. G., Contardi, C., Coppola, M., Costagliola, C., Cristofolini, R., Cucciniello, P., D'Aloisio, R., De Bernardo, M., De Filippis, Alessandro, Dell'Omo, Roberto, Di Paola, I., Dell'Acqua, Massimiliano, Franco, Alessio, Grassi, M. O., Gregori, G., Gusson, E., Lattanzio, R., Lanzetta, P., Longo, A., Marchini, G., Marolo, P., Mastropasqua, R., Bertoldo, G. M., Monteleone, Giorgio, Ortisi, E., Parisi, Giuseppe, Parrulli, S., Pucci, P., Pastore, M. R., Reibaldi, M., Rizzo, Stanislao, Romano, Federica, Rosa, N., Sarao, V., Scarpa, G., Scorcia, V., Scupola, Andrea, Staurenghi, G., Sunseri Trapani, V., Tognetto, D., Trabucchi, G., Vaccaro, S., Vadala, M., Veritti, D., Vinciguerra, A. L., Zanzottera, E. C., Giannarelli D., Longo R., Bertuzzi F., De Filippis A., Dell'Omo R., Dell'Acqua M., Franco A., Monteleone G., Parisi G., Rizzo S. (ORCID:0000-0001-6302-063X), Romano F., and Scupola A.
- Abstract
Purpose: To test reliability and reproducibility of ESASO morphologic OCT-based classification of diabetic maculopathy (DM). Methods: This is a multi-center cross-sectional study including a coordination center (CC) and 18 participating centers (PCs). After instruction on the correct use of ESASO Classification, the validation process was carried out in two consecutive stages. In the first retrospective phase, we evaluated the concordance between PCs and CC in the staging of OCT images collected during PCs’ daily activity (608 images). In a second prospective phase, we analyzed the inter-observer agreement of staging assigned by each PCs to OCT images selected by the CC (22 images). Results: The overall concordance achieved in the retrospective phase was 89.8% (Kappa = 0.83 (95% CI: 0.78–0.87); p<0.0001). In 99.5% of cases, concordance did not differ by more than one stage. In the prospective phase, PCs reached an inter-operator agreement of 93.0% (Krippendorff's Alpha = 0.953, 95% CI: 0.929–0.977, p<0.0001). Any discrepancy among the 22 images was within one stage. Conclusion: The results achieved in this study confirm that ESASO OCT-based Classification can be considered as an easy and reproducible method to stage DM during clinical practice. A diffused use of a common and validated method to describe the progression of retinal damage in DM may offer several clinical and scientific advantages.
- Published
- 2023
3. Phosphorus acquisition efficiency and transcriptomic changes of maize plants treated with different humates
- Author
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Santoro, V., Stevanato, P., Francioso, O., Zaccone, C., Schiavon, M., Bertoldo, G., Borella, M., della Lucia, M. C., and Nardi, S.
- Subjects
humic sustances ,Phosphorus, humic sustances, Zea mays, biostimulant ,Phosphorus ,Zea mays ,biostimulant - Published
- 2022
4. Seasonal influenza: Knowledge, attitude and vaccine uptake among adults with chronic conditions in Italy
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Bertoldo G, Pesce A, Pepe A, Pelullo CP, Di Giuseppe G, Collaborative Working Group., Bertoldo, G, Pesce, A, Pepe, A, Pelullo, Cp, Di Giuseppe, G, and Collaborative Working, Group.
- Published
- 2019
5. Designing Studies and Evaluating Research Results: Type M and Type S Errors for Pearson Correlation Coefficient
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Bertoldo G, Zandonella Callegher C, and Altoè G
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bepress|Life Sciences|Research Methods in Life Sciences ,bepress|Social and Behavioral Sciences|Psychology ,PsyArXiv|Meta-science ,bepress|Social and Behavioral Sciences|Psychology|Quantitative Psychology ,Type (model theory) ,Pearson product-moment correlation coefficient ,symbols.namesake ,PsyArXiv|Social and Behavioral Sciences ,PsyArXiv|Social and Behavioral Sciences|Psychology, other ,Statistics ,symbols ,bepress|Social and Behavioral Sciences ,PsyArXiv|Social and Behavioral Sciences|Quantitative Methods ,Mathematics - Abstract
It is widely appreciated that many studies in psychological science suffer from low statistical power. One of the consequences of analyzing underpowered studies with thresholds of statistical significance is a high risk of finding exaggerated effect size estimates, in the right or the wrong direction. These inferential risks can be directly quantified in terms of Type M (magnitude) error and Type S (sign) error, which directly communicate the consequences of design choices on effect size estimation. Given a study design, Type M error is the factor by which a statistically significant effect is on average exaggerated. Type S error is the probability to find a statistically significant result in the opposite direction to the plausible one. Ideally, these errors should be considered during a prospective design analysis in the design phase of a study to determine the appropriate sample size. However, they can also be considered when evaluating studies’ results in a retrospective design analysis. In the present contribution, we aim to facilitate the considerations of these errors in the research practice in psychology. For this reason, we illustrate how to consider Type M and Type S errors in a design analysis using one of the most common effect size measures in psychology: Pearson correlation coefficient. We provide various examples and make the R functions freely available to enable researchers to perform design analysis for their research projects.
- Published
- 2020
6. Genetic expression and root architecture changes in Beta vulgaris L. treated with a leonardite-based formulate
- Author
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Barone, V., Bertoldo, G., Magro, F., Stevanato, P., Broccanello, C., Puglisi, I., Baglieri, A., Cagnin, M., Moro, Matteo, Nardi, S., and Concheri, G.
- Published
- 2018
7. Morphological and molecular responses induced by sulfate and microalgae treatments in sugar beet
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Broccanello, C, Stevanato, P, Bertoldo, G, Barone, V, Cagnin, M, Lucini, L, Mandolino, G, Moliterni, Vmc, Orrù, L, Baglieri, A, Concheri, G, Squartini, A, and Nardi, S
- Published
- 2017
8. Morphological and molecular responses induced by microalgae extracts in Beta vulgaris L
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Barone, V., Baglieri, A., Stevanato, P., Broccanello, C., Bertoldo, G., Bertaggia, M., Cagnin, M., Pizzeghello, D., Moliterni, V. M. C., Mandolino, G., Fornasier, F., Squartini, A., Nardi, S., and Concheri, G
- Published
- 2017
9. Ionomic and molecular responses of sugar beet to changes in sulfate availability
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Stevanato, P, Moliterni, Vmc, Mandolino, G, Broccanello, C, Barone, V, Bertaggia, M, Cagnin, M, Bertoldo, G, Pizzeghello, D, Baglieri, A, Squartini, A, Concheri, G, and Nardi, S
- Published
- 2016
10. Root morphological and molecular responses induced by microalgae extracts in sugar beet (Beta vulgaris L.)
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Barone, V., primary, Baglieri, A., additional, Stevanato, Piergiorgio, additional, Broccanello, C., additional, Bertoldo, G., additional, Bertaggia, M., additional, Cagnin, M., additional, Pizzeghello, D., additional, Moliterni, V. M. C., additional, Mandolino, G., additional, Fornasier, F., additional, Squartini, A., additional, Nardi, S., additional, and Concheri, G., additional
- Published
- 2017
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11. Root morphological and molecular responses induced by microalgae extracts in sugar beet (<italic>Beta vulgaris</italic> L.).
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Barone, V., Baglieri, A., Stevanato, Piergiorgio, Broccanello, C., Bertoldo, G., Bertaggia, M., Cagnin, M., Pizzeghello, D., Moliterni, V. M. C., Mandolino, G., Fornasier, F., Squartini, A., Nardi, S., and Concheri, G.
- Abstract
The characterization of nutrient and biostimulant effects in crops is complex and needs rigorous evaluations. In this study, we evaluated morphological and molecular responses induced by microalgae (
Chlorella vulgaris andScenedesmus quadricauda ) extracts inBeta vulgaris L. The two microalgae extracts were firstly characterized by CNS, Fourier transform infrared spectroscopic analysis (FT-IR), and carbon-13 nuclear magnetic resonance (13 C NMR). Seedlings were grown in Hoagland’s solution under controlled conditions. After 5 days of growth, 2 mL L−1 (1 mg Corg L−1 ) and 4 mL L−1 (2 mg Corg L−1 ) of the two microalgae extracts were added to the Hoagland solution. Roots were sampled 36 h after treatments. Inductively coupled plasma spectrometry (ICP-OES) and nanofluidic real-time PCR (OpenArray system) were used for sample profiling. Fifty-three sugar beet genes putatively involved in sulfate starvation were tested in treated and untreated samples. Root morphological traits were measured by means of a scanner-based image analysis system. Multivariate statistical analysis revealed no significant changes in the ionomic profile of Hoagland’s solutions treated with the two microalgae extracts with respect to that of the untreated solution. At the molecular level, microalgae extract supplies upregulated many of the evaluated genes. Functional categorization revealed these genes to be related to various biological pathways and processes including primary and secondary metabolism and intracellular transport. At the morphological level, the treated seedlings showed significantly higher values for root traits related to soil exploration and nutrient uptake, such as total root length, fine root length (diameter < 0.5 mm), and number of root tips, than the untreated plants. These data indicate that microalgae extracts have biostimulant effects on the expression of root traits and genes related to nutrient acquisition in sugar beet. [ABSTRACT FROM AUTHOR]- Published
- 2018
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12. Valutazione morfo-funzionale delle membrane epiretiniche dopo vitrectomia
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Bertoldo, G., Borin, S., Mansoldo, C., and Marchini, Giorgio
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vitrectomia ,membrane epiretiniche - Published
- 2006
13. Studio OCT dello spessore delle fibre nervose nella popolazione normale
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Borin, S., Peruzzini, L., Bertoldo, G., Mansoldo, C, and Marchini, Giorgio
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OCT ,fibre nervose - Published
- 2006
14. Oderzo, via Savonarola (area SO.PRI.T. 1990). Uno scavo stratigrafico d’urgenza in ambito protourbano (nota preliminare)
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Balista, C., Bagolan, M., Bertoldo, G., Busana, MARIA STELLA, Cipriano, S., Marcassa, P., RUTA SERAFINI, A., and Tagliacozzo, A.
- Published
- 1992
15. Diode laser, vitrectomy and intravitreal triamcinolone. A comparative study for the treatment of diffuse non tractional diabetic macular edema
- Author
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Parolini, B, primary, Panozzo, G, additional, Gusson, E, additional, Pinackatt, S, additional, Bertoldo, G, additional, Rottini, S, additional, and Pignatto, S, additional
- Published
- 2004
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16. Diabetic macular edema: an OCT-based classification
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Panozzo, G, primary, Parolini, B, additional, Gusson, E, additional, Mercanti, A, additional, Pinackatt, S, additional, Bertoldo, G, additional, and Pignatto, S, additional
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- 2004
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17. A dual-omics approach for profiling plant responses to biostimulant applications under controlled and field conditions
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Ali Baghdadi, Maria Cristina Della Lucia, Matteo Borella, Giovanni Bertoldo, Samathmika Ravi, Walter Zegada-Lizarazu, Claudia Chiodi, Elena Pagani, Christian Hermans, Piergiorgio Stevanato, Serenella Nardi, Andrea Monti, Francesca Mangione, Baghdadi A., Della Lucia M.C., Borella M., Bertoldo G., Ravi S., Zegada-Lizarazu W., Chiodi C., Pagani E., Hermans C., Stevanato P., Nardi S., Monti A., and Mangione F.
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Ascophyllum nodosum ,biostimulant ,crop yield ,plant physiology ,tomato ,transcriptome ,Plant Science - Abstract
A comprehensive approach using phenomics and global transcriptomics for dissecting plant response to biostimulants is illustrated with tomato (Solanum lycopersicum cv. Micro-Tom and Rio Grande) plants cultivated in the laboratory, greenhouse, and open field conditions. Biostimulant treatment based on an Ascophyllum nodosum extract (ANE) was applied as a foliar spray with two doses (1 or 2 l ha-1) at three different phenological stages (BBCH51, BBCH61, and BBCH65) during the flowering phase. Both ANE doses resulted in greater net photosynthesis rate, stomatal conductance, and fruit yield across all culture conditions. A global transcriptomic analysis of leaves from plants grown in the climate chamber, revealed a greater number of differentially expressed genes (DEGs) with the low ANE dose compared to the greater one. The second and third applications induced broader transcriptome changes compared to the first one, indicating a cumulative treatment effect. The functional enrichment analysis of DEGs highlighted pathways related to stimulus-response and photosynthesis, consistent with the morpho-physiological observations. This study is the first comprehensive dual-omics approach for profiling plant responses to biostimulants across three different culture conditions.
- Published
- 2022
18. RNA-seq analyses on gametogenic tissues of alfalfa (Medicago sativa) revealed plant reproduction- and ploidy-related genes.
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Palumbo F, Gabelli G, Pasquali E, Vannozzi A, Farinati S, Draga S, Ravi S, Della Lucia MC, Bertoldo G, and Barcaccia G
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- Transcriptome, Ploidies, Gene Expression Regulation, Plant, Genes, Plant, Reproduction genetics, Flowers genetics, Flowers growth & development, Gene Expression Profiling, Medicago sativa genetics, RNA-Seq
- Abstract
Background: In alfalfa (Medicago sativa), the coexistence of interfertile subspecies (i.e. sativa, falcata and coerulea) characterized by different ploidy levels (diploidy and tetraploidy) and the occurrence of meiotic mutants capable of producing unreduced (2n) gametes, have been efficiently combined for the establishment of new polyploids. The wealth of agronomic data concerning forage quality and yield provides a thorough insight into the practical benefits of polyploidization. However, many of the underlying molecular mechanisms regarding gene expression and regulation remained completely unexplored. In this study, we aimed to address this gap by examining the transcriptome profiles of leaves and reproductive tissues, corresponding to anthers and pistils, sampled at different time points from diploid and tetraploid Medicago sativa individuals belonging to progenies produced by bilateral sexual polyploidization (dBSP and tBSP, respectively) and tetraploid individuals stemmed from unilateral sexual polyploidization (tUSP)., Results: Considering the crucial role played by anthers and pistils in the reduced and unreduced gametes formation, we firstly analyzed the transcriptional profiles of the reproductive tissues at different stages, regardless of the ploidy level and the origin of the samples. By using and combining three different analytical methodologies, namely weighted-gene co-expression network analysis (WGCNA), tau (τ) analysis, and differentially expressed genes (DEGs) analysis, we identified a robust set of genes and transcription factors potentially involved in both male sporogenesis and gametogenesis processes, particularly in crossing-over, callose synthesis, and exine formation. Subsequently, we assessed at the same floral stage, the differences attributable to the ploidy level (tBSP vs. dBSP) or the origin (tBSP vs. tUSP) of the samples, leading to the identification of ploidy and parent-specific genes. In this way, we identified, for example, genes that are specifically upregulated and downregulated in flower buds in the comparison between tBSP and dBSP, which could explain the reduced fertility of the former compared to the latter materials., Conclusions: While this study primarily functions as an extensive investigation at the transcriptomic level, the data provided could represent not only a valuable original asset for the scientific community but also a fully exploitable genomic resource for functional analyses in alfalfa., (© 2024. The Author(s).)
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- 2024
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19. Determining the hierarchical order by which intestinal tract, administered diet, and individual relay can shape the gut microbiome of fattening quails.
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Bertoldo G, Broccanello C, Tondello A, Cappellozza S, Saviane A, Kovitvadhi A, Concheri G, Cullere M, Stevanato P, Zotte AD, and Squartini A
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- Animals, Coturnix physiology, Diet, Animal Feed analysis, Quail physiology, Gastrointestinal Microbiome
- Abstract
A bacterial metabarcoding approach was used to compare the microbiome composition of caecal and faecal samples from fattening Japanese quails (Coturnix coturnix japonica) fed three different diet regimes. The tested feedstuffs included (1) a commercial diet for fattening quails, (2) a commercial diet containing 12% full-fat silkworm (Bombyx mori) pupae meal, and (3) a commercial diet containing 12% defatted silkworm pupae meal. The aim of the experiment was to verify the relative effect of three variables (diet type, gut tract comparing caecum to rectum, and individual animal) in determining the level of bacterial community dissimilarity to rank the relevance of each of the three factors in affecting and shaping community composition. To infer such ranking, the communities resulting from the high-throughput sequencing from each sample were used to calculate the Bray-Curtis distances in all the pairwise combinations, whereby identical communities would score 0 and totally different ones would yield the maximum distance, equal to 1. The results indicated that the main driver of divergence was the gut tract, as distances between caecal and faecal samples were higher on average, irrespective of diet composition, which scored second in rank, and of whether they had been sampled from the same individual, which was the least effective factor. Simpson's species diversity indexes was not significantly different when comparing tracts or diets, while community evenness was reduced in full-fat silkworm diet-fed animals. The identities of the differentially displayed taxa that were statistically significant as a function of gut tract and diet regimen are discussed in light of their known physiological and functional traits., Competing Interests: The authors have declared that no competing interests exist., (Copyright: © 2024 Bertoldo et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
- Published
- 2024
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20. Asymmetrical high-flow nasal cannula performs similarly to standard interface in patients with acute hypoxemic post-extubation respiratory failure: a pilot study.
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Boscolo A, Pettenuzzo T, Zarantonello F, Sella N, Pistollato E, De Cassai A, Congedi S, Paiusco I, Bertoldo G, Crociani S, Toma F, Mormando G, Lorenzoni G, Gregori D, and Navalesi P
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- Adult, Humans, Pilot Projects, Cannula, Dyspnea, Oxygen, Airway Extubation, Respiratory Insufficiency etiology, Respiratory Insufficiency therapy
- Abstract
Background: Standard high-flow nasal cannula (HFNC) is a respiratory support device widely used to manage post-extubation hypoxemic acute respiratory failure (hARF) due to greater comfort, oxygenation, alveolar recruitment, humidification, and reduction of dead space, as compared to conventional oxygen therapy. On the contrary, the effects of the new asymmetrical HFNC interface (Optiflow® Duet system (Fisher & Paykel, Healthcare, Auckland, New Zealand) is still under discussion. Our aim is investigating whether the use of asymmetrical HFNC interface presents any relevant difference, compared with the standard configuration, on lung aeration (as assessed by end-expiratory lung impedance (EELI) measured by electrical impedance tomography (EIT)), diaphragm ultrasound thickening fraction (TFdi) and excursion (DE), ventilatory efficiency (estimated by corrected minute ventilation (MV)), gas exchange, dyspnea, and comfort., Methods: Pilot physiological crossover randomized controlled study enrolling 20 adults admitted to the Intensive Care unit, invasively ventilated for at least 24 h, and developing post-extubation hARF, i.e., PaO
2 /set FiO2 < 300 mmHg during Venturi mask (VM) within 120 min after extubation. Each HFNC configuration was applied in a randomized 60 min sequence at a flow rate of 60 L/min., Results: Global EELI, TFdi, DE, ventilatory efficiency, gas exchange and dyspnea were not significantly different, while comfort was greater during asymmetrical HFNC support, as compared to standard interface (10 [7-10] and 8 [7-9], p-value 0.044)., Conclusions: In post-extubation hARF, the use of the asymmetrical HFNC, as compared to standard HFNC interface, slightly improved patient comfort without affecting lung aeration, diaphragm activity, ventilatory efficiency, dyspnea and gas exchange., Clinical Trial Number: ClinicalTrial.gov., Registration Number: NCT05838326 (01/05/2023)., New & Noteworthy: The asymmetrical high-flow nasal cannula oxygen therapy (Optiflow® Duet system (Fisher & Paykel, Healthcare, Auckland, New Zealand) provides greater comfort as compared to standard interface; while their performance in term of lung aeration, diaphragm activity, ventilatory efficiency, dyspnea, and gas exchange is similar., (© 2024. The Author(s).)- Published
- 2024
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21. Phosphorus Acquisition Efficiency and Transcriptomic Changes in Maize Plants Treated with Two Lignohumates.
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Santoro V, Della Lucia MC, Francioso O, Stevanato P, Bertoldo G, Borella M, Ferrari E, Zaccone C, Schiavon M, Pizzeghello D, and Nardi S
- Abstract
Lignohumates are increasing in popularity in agriculture, but their chemistry and effects on plants vary based on the source and processing. The present study evaluated the ability of two humates (H1 and H2) to boost maize plant performance under different phosphorus (P) availability (25 and 250 μM) conditions in hydroponics, while understanding the underlying mechanisms. Humates differed in chemical composition, as revealed via elemental analysis, phenol and phytohormone content, and thermal and spectroscopic analyses. H1 outperformed H2 in triggering plant responses to low phosphorus by enhancing phosphatase and phytase enzymes, P acquisition efficiency, and biomass production. It contained higher levels of endogenous auxins, cytokinins, and abscisic acid, likely acting together to stimulate plant growth. H1 also improved the plant antioxidant capacity, thus potentially increasing plant resilience to external stresses. Both humates increased the nitrogen (N) content and acted as biostimulants for P and N acquisition. Consistent with the physiological and biochemical data, H1 upregulated genes involved in growth, hormone signaling and defense in all plants, and in P recycling particularly under low-P conditions. In conclusion, H1 showed promising potential for effective plant growth and nutrient utilization, especially in low-P plants, involving hormonal modulation, antioxidant enhancement, the stimulation of P uptake and P-recycling mechanisms.
- Published
- 2023
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22. Transcriptomic and physiological approaches to decipher cold stress mitigation exerted by brown-seaweed extract application in tomato.
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Borella M, Baghdadi A, Bertoldo G, Della Lucia MC, Chiodi C, Celletti S, Deb S, Baglieri A, Zegada-Lizarazu W, Pagani E, Monti A, Mangione F, Magro F, Hermans C, Stevanato P, and Nardi S
- Abstract
Chilling temperatures represent a challenge for crop species originating from warm geographical areas. In this situation, biostimulants serve as an eco-friendly resource to mitigate cold stress in crops. Tomato ( Solanum lycopersicum L.) is an economically important vegetable crop, but quite sensitive to cold stress, which it encounters in both open field and greenhouse settings. In this study, the biostimulant effect of a brown-seaweed extract (BSE) has been evaluated in tomato exposed to low temperature. To assess the product effects, physiological and molecular characterizations were conducted. Under cold stress conditions, stomatal conductance, net photosynthesis, and yield were significantly (p ≤ 0.05) higher in BSE-treated plants compared to the untreated ones. A global transcriptomic survey after BSE application revealed the impact of the BSE treatment on genes leading to key responses to cold stress. This was highlighted by the significantly enriched GO categories relative to proline (GO:0006560), flavonoids (GO:0009812, GO:0009813), and chlorophyll (GO:0015994). Molecular data were integrated by biochemical analysis showing that the BSE treatment causes greater proline, polyphenols, flavonoids, tannins, and carotenoids contents.The study highlighted the role of antioxidant molecules to enhance tomato tolerance to low temperature mediated by BSE-based biostimulant., Competing Interests: FMag is employed by Sipcam Italia S.p.A. belonging together with Sofbey SA to the Sipcam Oxon S.p.A. Group Pero, Italy. FMan is a former employer at Sipcam Italia S.p.A. belonging together with Sofbey SA to the Sipcam Oxon S.p.A. Group Pero, Italy. FMan was employed at Sipcam during the time of the study. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Borella, Baghdadi, Bertoldo, Della Lucia, Chiodi, Celletti, Deb, Baglieri, Zegada-Lizarazu, Pagani, Monti, Mangione, Magro, Hermans, Stevanato and Nardi.)
- Published
- 2023
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23. Transcriptome-Assisted SNP Marker Discovery for Phytophthora infestans Resistance in Solanum lycopersicum L.
- Author
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Deb S, Della Lucia MC, Ravi S, Bertoldo G, and Stevanato P
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- Disease Resistance genetics, Polymorphism, Single Nucleotide, Transcriptome, Plant Breeding, Plant Diseases genetics, Plant Diseases microbiology, Solanum lycopersicum genetics, Phytophthora infestans genetics, Solanum genetics, Solanum tuberosum genetics
- Abstract
Late blight, caused by oomycetes Phytophthora infestans is one of the most challenging fungal diseases to manage in tomato plants ( Solanum lycopersicum L.). Toward managing the disease, conventional breeding has successfully introgressed genetic loci conferring disease resistance from various wild relatives of tomato into commercial varieties. The cataloging of disease-associated SNP markers and a deeper understanding of disease-resistance mechanisms are needed to keep up with the demand for commercial varieties resistant against emerging pathogen strains. To this end, we performed transcriptome sequencing to evaluate the gene expression dynamics of tomato varieties, resistant and susceptible to Phytophthora infection. Further integrating the transcriptome dataset with large-scale public genomic data of varieties with known disease phenotypes, a panel of single nucleotide polymorphism (SNP) markers correlated with disease resistance was identified. These SNPs were then validated on 31 lines with contrasting phenotypes for late blight. The identified SNPs are located on genes coding for a putative cysteine-rich transmembrane module (CYSTM), Solyc09g098310, and a nucleotide-binding site-leucine-rich repeat protein, Solyc09g098100, close to the well-studied Ph-3 resistance locus known to have a role in plant immunity against fungal infections. The panel of SNPs generated by this study using transcriptome sequencing showing correlation with disease resistance across a broad set of plant material can be used as markers for molecular screening in tomato breeding.
- Published
- 2023
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24. Brown Seaweed Extract (BSE) Application Influences Auxin- and ABA-Related Gene Expression, Root Development, and Sugar Yield in Beta vulgaris L.
- Author
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Bertoldo G, Chiodi C, Della Lucia MC, Borella M, Ravi S, Baglieri A, Lucenti P, Ganasula BK, Mulagala C, Squartini A, Concheri G, Magro F, Campagna G, Stevanato P, and Nardi S
- Abstract
The molecular and phenotypic effects of a brown seaweed extract (BSE) were assessed in sugar beet ( Beta vulgaris L.). Transcript levels of BSE-treated and untreated plants were studied by RNA-seq and validated by quantitative real-time PCR analysis (RT-qPCR). Root morphology, sugar yield, and processing quality traits were also analyzed to better elucidate the treatment effects. RNA-seq revealed 1019 differentially expressed genes (DEGs) between the BSE-treated and untreated plants. An adjusted p -value < 0.1 and an absolute value of log2 (fold change) greater than one was used as criteria to select the DEGs. Gene ontology (GO) identified hormone pathways as an enriched biological process. Six DEGs involved in auxin and ABA pathways were validated using RT-qPCR. The phenotypic characterization indicated that BSE treatment led to a significant increase ( p < 0.05) in total root length and the length of fine roots of plants grown under hydroponics conditions. The sugar yield of plants grown under field conditions was higher ( p < 0.05) in the treated field plots compared with the control treatment, without impacting the processing quality. Our study unveiled the relevant effects of BSE application in regulating auxin- and ABA-related gene expression and critical traits related to sugar beet development and yield.
- Published
- 2023
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25. A dual-omics approach for profiling plant responses to biostimulant applications under controlled and field conditions.
- Author
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Baghdadi A, Della Lucia MC, Borella M, Bertoldo G, Ravi S, Zegada-Lizarazu W, Chiodi C, Pagani E, Hermans C, Stevanato P, Nardi S, Monti A, and Mangione F
- Abstract
A comprehensive approach using phenomics and global transcriptomics for dissecting plant response to biostimulants is illustrated with tomato ( Solanum lycopersicum cv. Micro-Tom and Rio Grande) plants cultivated in the laboratory, greenhouse, and open field conditions. Biostimulant treatment based on an Ascophyllum nodosum extract (ANE) was applied as a foliar spray with two doses (1 or 2 l ha
-1 ) at three different phenological stages (BBCH51, BBCH61, and BBCH65) during the flowering phase. Both ANE doses resulted in greater net photosynthesis rate, stomatal conductance, and fruit yield across all culture conditions. A global transcriptomic analysis of leaves from plants grown in the climate chamber, revealed a greater number of differentially expressed genes (DEGs) with the low ANE dose compared to the greater one. The second and third applications induced broader transcriptome changes compared to the first one, indicating a cumulative treatment effect. The functional enrichment analysis of DEGs highlighted pathways related to stimulus-response and photosynthesis, consistent with the morpho-physiological observations. This study is the first comprehensive dual-omics approach for profiling plant responses to biostimulants across three different culture conditions., Competing Interests: Author Francesca Mangione is employed by Sipcam Italia S.p.A. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. No funds from a commercial party were received in support of this article. No benefits in any form have been or will be received from a commercial party related directly or indirectly to the subject of this manuscript. No influence on the interpretation of the data and writing of the article was exerted by a commercial party., (Copyright © 2022 Baghdadi, Della Lucia, Borella, Bertoldo, Ravi, Zegada-Lizarazu, Chiodi, Pagani, Hermans, Stevanato, Nardi, Monti and Mangione.)- Published
- 2022
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26. Bacterial endophytes as indicators of susceptibility to Cercospora Leaf Spot (CLS) disease in Beta vulgaris L.
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Broccanello C, Ravi S, Deb S, Bolton M, Secor G, Richards C, Maretto L, Lucia MCD, Bertoldo G, Orsini E, Ronquillo-López MG, Concheri G, Campagna G, Squartini A, and Stevanato P
- Subjects
- Cercospora, Endophytes genetics, Plant Breeding, Plant Diseases genetics, Plant Diseases microbiology, RNA, Ribosomal, 16S genetics, Sugars, Ascomycota genetics, Beta vulgaris genetics
- Abstract
The fungus Cercospora beticola causes Cercospora Leaf Spot (CLS) of sugar beet (Beta vulgaris L.). Despite the global importance of this disease, durable resistance to CLS has still not been obtained. Therefore, the breeding of tolerant hybrids is a major goal for the sugar beet sector. Although recent studies have suggested that the leaf microbiome composition can offer useful predictors to assist plant breeders, this is an untapped resource in sugar beet breeding efforts. Using Ion GeneStudio S5 technology to sequence amplicons from seven 16S rRNA hypervariable regions, the most recurring endophytes discriminating CLS-symptomatic and symptomless sea beets (Beta vulgaris L.ssp. maritima) were identified. This allowed the design of taxon-specific primer pairs to quantify the abundance of the most representative endophytic species in large naturally occurring populations of sea beet and subsequently in sugar beet breeding genotypes under either CLS symptomless or infection stages using qPCR. Among the screened bacterial genera, Methylobacterium and Mucilaginibacter were found to be significantly (p < 0.05) more abundant in symptomatic sea beets with respect to symptomless. In cultivated sugar beet material under CLS infection, the comparison between resistant and susceptible genotypes confirmed that the susceptible genotypes hosted higher contents of the above-mentioned bacterial genera. These results suggest that the abundance of these species can be correlated with increased sensitivity to CLS disease. This evidence can further prompt novel protocols to assist plant breeding of sugar beet in the pursuit of improved pathogen resistance., (© 2022. The Author(s).)
- Published
- 2022
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27. SNP Alleles Associated With Low Bolting Tendency in Sugar Beet.
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Ravi S, Campagna G, Della Lucia MC, Broccanello C, Bertoldo G, Chiodi C, Maretto L, Moro M, Eslami AS, Srinivasan S, Squartini A, Concheri G, and Stevanato P
- Abstract
The identification of efficient molecular markers related to low bolting tendency is a priority in sugar beet ( Beta vulgaris L.) breeding. This study aimed to identify SNP markers associated with low bolting tendency by establishing a genome-wide association study. An elaborate 3-year field trial comprising 13 sugar beet lines identified L14 as the one exhibiting the lowest bolting tendency along with an increased survival rate after autumnal sowing. For SNP discovery following phenotyping, contrasting phenotypes of 24 non-bolting and 15 bolting plants of the L14 line were sequenced by restriction site-associated DNA sequencing (RAD-seq). An association model was established with a set of 10,924 RAD-based single nucleotide polymorphism (SNP) markers. The allelic status of the most significantly associated SNPs ranked based on their differential allelic status between contrasting phenotypes ( p < 0.01) was confirmed on three different validation datasets comprising diverse sugar beet lines and varieties adopting a range of SNP detection technologies. This study has led to the identification of SNP_36780842 and SNP_48607347 linked to low bolting tendency and can be used for marker-assisted breeding and selection in sugar beet., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Ravi, Campagna, Della Lucia, Broccanello, Bertoldo, Chiodi, Maretto, Moro, Eslami, Srinivasan, Squartini, Concheri and Stevanato.)
- Published
- 2021
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28. Endophytic Microbiome Responses to Sulfur Availability in Beta vulgaris (L.).
- Author
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Bertoldo G, Della Lucia MC, Squartini A, Concheri G, Broccanello C, Romano A, Ravi S, Cagnin M, Baglieri A, and Stevanato P
- Subjects
- Metagenome, Beta vulgaris microbiology, Endophytes, Microbiota, Sulfur
- Abstract
Sulfur is an essential plant macronutrient, and its adequate supply allows an efficient root storage and sugar extractability in sugar beets ( Beta vulgaris L.). In this study, we investigated the effect of changes in sulfur availability on the endophytic community structure of sugar beets. Plants were hydroponically grown in a complete nutrient solution (S-supplied), a nutrient solution without MgSO
4 (S-deprived), and a nutrient solution without MgSO4 for six days and resupplied with 100 μM MgSO4 for 48 h (S-resupplied). The sulfur status was monitored by inductively coupled plasma ICP-OES, and combustion analysis together with the evaluation of microRNA395 as a biomarker for sulfate status. Metabarcoding of the bacterial 16S rRNA gene was carried out in order to determine leaf endophytic community structure. The Shannon diversity index significantly differed ( p < 0.05) between sulfate-supplied and sulfate-deprived seedlings. Validation by Real-Time PCR showed a significant increase ( p < 0.05) of Burkholderia spp. in sulfate-deprived plants as compared to sulfate-supplied ones. The study sheds new light on the effects of nutrient deficiency on the microbiome of sugar beet plants.- Published
- 2021
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29. Novel Effects of Leonardite-Based Applications on Sugar Beet.
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Della Lucia MC, Bertoldo G, Broccanello C, Maretto L, Ravi S, Marinello F, Sartori L, Marsilio G, Baglieri A, Romano A, Colombo M, Magro F, Campagna G, Concheri G, Squartini A, and Stevanato P
- Abstract
The present study aimed to explore the effects of foliar application of a leonardite-based product on sugar beet ( Beta vulgaris L.) plants grown in the field. The approach concerned the evaluation of the community compositional structure of plant endophytic bacteria through a metabarcoding approach, the expression level of a gene panel related to hormonal metabolism and signaling, and the main sugar beet productivity traits. Results indicated that plants treated with leonardite (dosage of 2,000 ml ha
-1 , dilution 1:125, 4 mg C l-1 ) compared with untreated ones had a significant increase ( p < 0.05) in (i) the abundance of Oxalicibacterium spp., recognized to be an endophyte bacterial genus with plant growth-promoting activity; (ii) the expression level of LAX2 gene, coding for auxin transport proteins; and (iii) sugar yield. This study represents a step forward to advance our understanding of the changes induced by leonardite-based biostimulant in sugar beet., Competing Interests: FM was employed by company Sipcam Oxon S.p.A. GCa was employed by company COPROB. The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Della Lucia, Bertoldo, Broccanello, Maretto, Ravi, Marinello, Sartori, Marsilio, Baglieri, Romano, Colombo, Magro, Campagna, Concheri, Squartini and Stevanato.)- Published
- 2021
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30. Replicating patterns of prospect theory for decision under risk.
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Ruggeri K, Alí S, Berge ML, Bertoldo G, Bjørndal LD, Cortijos-Bernabeu A, Davison C, Demić E, Esteban-Serna C, Friedemann M, Gibson SP, Jarke H, Karakasheva R, Khorrami PR, Kveder J, Andersen TL, Lofthus IS, McGill L, Nieto AE, Pérez J, Quail SK, Rutherford C, Tavera FL, Tomat N, Reyn CV, Većkalov B, Wang K, Yosifova A, Papa F, Rubaltelli E, Linden SV, and Folke T
- Subjects
- Adolescent, Adult, Aged, Aged, 80 and over, Cross-Cultural Comparison, Female, Humans, Male, Middle Aged, Reproducibility of Results, Risk, Risk-Taking, Young Adult, Decision Making, Psychological Theory
- Abstract
Prospect theory is among the most influential frameworks in behavioural science, specifically in research on decision-making under risk. Kahneman and Tversky's 1979 study tested financial choices under risk, concluding that such judgements deviate significantly from the assumptions of expected utility theory, which had remarkable impacts on science, policy and industry. Though substantial evidence supports prospect theory, many presumed canonical theories have drawn scrutiny for recent replication failures. In response, we directly test the original methods in a multinational study (n = 4,098 participants, 19 countries, 13 languages), adjusting only for current and local currencies while requiring all participants to respond to all items. The results replicated for 94% of items, with some attenuation. Twelve of 13 theoretical contrasts replicated, with 100% replication in some countries. Heterogeneity between countries and intra-individual variation highlight meaningful avenues for future theorizing and applications. We conclude that the empirical foundations for prospect theory replicate beyond any reasonable thresholds.
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- 2020
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31. Enhancing Statistical Inference in Psychological Research via Prospective and Retrospective Design Analysis.
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Altoè G, Bertoldo G, Zandonella Callegher C, Toffalini E, Calcagnì A, Finos L, and Pastore M
- Abstract
In the past two decades, psychological science has experienced an unprecedented replicability crisis, which has uncovered several issues. Among others, the use and misuse of statistical inference plays a key role in this crisis. Indeed, statistical inference is too often viewed as an isolated procedure limited to the analysis of data that have already been collected. Instead, statistical reasoning is necessary both at the planning stage and when interpreting the results of a research project. Based on these considerations, we build on and further develop an idea proposed by Gelman and Carlin (2014) termed "prospective and retrospective design analysis." Rather than focusing only on the statistical significance of a result and on the classical control of type I and type II errors, a comprehensive design analysis involves reasoning about what can be considered a plausible effect size. Furthermore, it introduces two relevant inferential risks: the exaggeration ratio or Type M error (i.e., the predictable average overestimation of an effect that emerges as statistically significant) and the sign error or Type S error (i.e., the risk that a statistically significant effect is estimated in the wrong direction). Another important aspect of design analysis is that it can be usefully carried out both in the planning phase of a study and for the evaluation of studies that have already been conducted, thus increasing researchers' awareness during all phases of a research project. To illustrate the benefits of a design analysis to the widest possible audience, we use a familiar example in psychology where the researcher is interested in analyzing the differences between two independent groups considering Cohen's d as an effect size measure. We examine the case in which the plausible effect size is formalized as a single value, and we propose a method in which uncertainty concerning the magnitude of the effect is formalized via probability distributions. Through several examples and an application to a real case study, we show that, even though a design analysis requires significant effort, it has the potential to contribute to planning more robust and replicable studies. Finally, future developments in the Bayesian framework are discussed., (Copyright © 2020 Altoè, Bertoldo, Zandonella Callegher, Toffalini, Calcagnì, Finos and Pastore.)
- Published
- 2020
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32. Expression Profiling of Candidate Genes in Sugar Beet Leaves Treated with Leonardite-Based Biostimulant.
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Hajizadeh HS, Heidari B, Bertoldo G, Della Lucia MC, Magro F, Broccanello C, Baglieri A, Puglisi I, Squartini A, Campagna G, Concheri G, Nardi S, and Stevanato P
- Abstract
Leonardite-based biostimulants are a large class of compounds, including humic acid substances. Foliar application of biostimulants at field level improves plant growth, yield and quality through metabolic changes and stimulation of plant proton pumps. The present study aimed at identifying optimum dosage of BLACKJAK, a humic acid-based substance, which is able to modify genes involved in sugar beet growth. Thirty-three genes belonging to various biochemical pathway categories were tested in leaves of treated sugar beet ( Beta vulgaris L.) samples to assess gene expression profiling in response to BLACKJAK. Seedlings of a diploid and multigerm variety were grown in plastic pots and sprayed with two dilutions of BLACKJAK (dilution 1:500-1.0 mg C L
-1 and dilution 1:1000-0.5 mg C L-1 ). Leaf samples were collected after 24, 48, and 72 h treatment with BLACKJAK for each dilution. RNA was extracted and the quantification of gene expression was performed while using an OpenArray platform. Results of analysis of variance demonstrated that, 15 genes out of a total of 33 genes tested with OpenArray qPCR were significantly affected by treatment and exposure time. Analysis for annotation of gene products and pathways revealed that genes belonging to the mitochondrial respiratory pathways, nitrogen and hormone metabolisms, and nutrient uptake were up-regulated in the BLACKJAK treated samples. Among the up-regulated genes, Bv_PHT2;1 and Bv_GLN1 expression exerted a 2-fold change in 1:1000 and 1:500 BLACKJAK concentrations. Overall, the gene expression data in the BLACKJAK treated leaves demonstrated the induction of plant growth-related genes that were contributed almost to amino acid and nitrogen metabolism, plant defense system, and plant growth.- Published
- 2019
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33. Molecular and Morphological Changes Induced by Leonardite-based Biostimulant in Beta vulgaris L.
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Barone V, Bertoldo G, Magro F, Broccanello C, Puglisi I, Baglieri A, Cagnin M, Concheri G, Squartini A, Pizzeghello D, Nardi S, and Stevanato P
- Abstract
Humic substances extracted from leonardite are widely considered to be bioactive compounds, influencing the whole-plant physiology and the crop yield. The aim of this work was to evaluate the effect of a new formulate based on leonardite in the early stage of growth of sugar beet ( Beta vulgaris L.). A commercial preparation of leonardite (BLACKJAK) was characterized by ionomic analysis, solid-state
13 C MAS NMR spectroscopy. Seedlings of sugar beet were grown in Hoagland's solution under controlled conditions. After five days of growth, an aliquot of the concentrated BLACKJAK was added to the solution to obtain a final dilution of 1:1000 (0.5 mg C L-1 ). The sugar beet response in the early stage of growth was determined by evaluating root morphological traits as well as the changes in the expression of 53 genes related to key morphophysiological processes. Root morphological traits, such as total root length, fine root length (average diameter < 0.5 mm), and number of root tips, were significantly ( p < 0.001) increased in plants treated with BLACKJAK, compared to the untreated plants at all sampling times. At the molecular level, BLACKJAK treatment upregulated many of the evaluated genes. Moreover, both Real Time PCR and digital PCR showed that genes involved in hormonal response, such as PIN, ARF3, LOGL 10, GID1, and BRI1, were significantly ( p < 0.05) upregulated by treatment with BLACKJAK. Our study provides essential information to understand the effect of a leonardite-based formulate on plant growth hormone metabolism, although the molecular and physiological basis for these complicated regulatory mechanisms deserve further investigations., Competing Interests: The authors declare no conflict of interest.- Published
- 2019
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34. Innovative Approaches to Evaluate Sugar Beet Responses to Changes in Sulfate Availability.
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Stevanato P, Broccanello C, Moliterni VMC, Mandolino G, Barone V, Lucini L, Bertoldo G, Bertaggia M, Cagnin M, Pizzeghello D, Baglieri A, Squartini A, Concheri G, and Nardi S
- Abstract
In this study, a system based on omics profiling was set-up for sugar beet ( Beta vulgaris L. subsp. vulgaris ) evaluation after changes in sulfate availability. Seedlings were grown on sulfate-deprived Hoagland solution. Six days after germination, 100 μM MgSO
4 was added to the solution. Root samples were collected 36 h after treatments. WinRHIZO root-scanning approach was used for the automated image analysis of plant root morphology. Inductively Coupled Plasma Spectrometry (ICP-OES) and quadrupole-time-of-flight mass spectrometry (Q-TOF) were used for ionomic and metabolic analysis, respectively. Nanofluidic real-time PCR (OpenArray system) was used for molecular profiling. OpenArray chips were designed with TaqMan probes for 53 sugar beet genes putatively involved in sulfate nutrition. At morphological level treated seedlings showed significantly higher values ( P < 0.01) than untreated plants for root traits related to soil exploration and nutrient uptake, such as total root length, fine roots length and root tips number. ICP-OES, Q-TOF and transcriptomic data revealed changes due to sulfate availability in sugar beet samples. Two key results are highlighted in sulfate-supplied roots and leaves. Firstly, high expression levels of auxin efflux carrier component 1 (PIN) and 5-phosphoribosyl-anthranilate, precursor of tryptophan and auxin synthesis, were observed in roots. Secondly, high levels of 2-Cys peroxiredoxin BAS1, chloroplastic, thioredoxin reductase (NADPH) and cysteine synthase, chloroplastic/chromoplastic, O- acetylserine sulfhydrylase, involved in protection against oxidative stress and cysteine synthase activity, respectively, were observed in leaves. Based on our findings, the combination of evaluated omics approaches could become a key system for the evaluation of the nutritional status of sugar beet under different nutrient availability conditions.- Published
- 2018
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35. [Presence-absence sampling plan for Oligonychus yothersi (McGregor) (Acari: Tetranychidae) on mate-tea orchard].
- Author
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Gouvea Ad, Bertoldo G, and Alves LF
- Subjects
- Animals, Population Density, Ilex paraguariensis parasitology, Plant Leaves parasitology, Tetranychidae
- Abstract
Mite infestations to the culture of mate-tea frequently causes losses by the premature fall of the leaves. So, it is necessary to monitor the population of these arthropods, and to adopt management strategies for their control. The objective of this research was to evaluate the trustworthiness of presence-absence sampling for Oligonychus yothersi (McGregor) on mate-tea orchard. This study was conducted in Cascavel, Paraná State, from April 2001 to July 2000, in a mate-tea tree commercial plantation. Biweekly sampling of 240 leaves were collected in different parts of 10 plants, and the number of mites was counted. The aggregation pattern was determined through the coefficients a and b of Taylor's power law. The proportion of infested leaves and the number of required samples were estimated through mathematical model. The mite O. yothersi presented aggregate distribution. The proportion of infested leaves calculated by means of mathematical model showed to be a trustworthy parameter to estimate the population density of the mite. The number of required samples was small, making feasible the practical application of the presence-absence sampling method for O. yothersi in the culture of mate-tea.
- Published
- 2007
- Full Text
- View/download PDF
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