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2. The 50 distal amino acids of the 2A(HP) homing protein of Grapevine fanleaf virus elicit a hypersensitive reaction on Nicotiana occidentalis

Author

Martin, Isabelle, Vigne, Emmanuelle, Berthold, François, Komar, Véronique, LEMAIRE, Olivier, Fuchs, Marc, Schmitt-Keichinger, Corinne, Université Pierre et Marie Curie - Paris 6 - UFR de Médecine Pierre et Marie Curie (UPMC), Université Pierre et Marie Curie - Paris 6 (UPMC), Santé de la vigne et qualité du vin (SVQV), Institut National de la Recherche Agronomique (INRA)-Université de Strasbourg (UNISTRA), Bioénergétique et Ingénierie des Protéines (BIP ), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Section of Plant Pathology and Plant-Microbe Biology, School of Integrative Plant Science, New York State Agricultural Experiment Station, Cornell University, Cornell University [New York], INRA, Moet Chandon, Comite Interprofessionnel du Vin de Champagne, Bureau Interprofessionnel des Vins de Bourgogne, Conseil Interprofessionnel des Vins d'Alsace, School of Integrative Plant Science [CALS], College of Agriculture and Life Sciences [Cornell University] (CALS), and Cornell University [New York]-Cornell University [New York]

Subjects
defence, Secoviridae, Nepovirus, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, Original Articles, Genome, Viral, reverse genetics, Viral Proteins, symptom determinant, avirulence factor, hypersensitive reaction, Tobacco, Amino Acids, ComputingMilieux_MISCELLANEOUS, Plant Diseases
Abstract

International audience; Avirulence factors are critical for the arm's race between a virus and its host in determining incompatible reactions. The response of plants to viruses from the genus Nepovirus in the family Secoviridae, including Grapevine fanleaf virus (GFLV), is well characterized, although the nature and characteristics of the viral avirulence factor remain elusive. By using infectious clones of GFLV strains F13 and GHu in a reverse genetics approach with wild-type, assortant and chimeric viruses, the determinant of necrotic lesions caused by GFLV-F13 on inoculated leaves of Nicotiana occidentalis was mapped to the RNA2-encoded protein 2A(HP), particularly to its 50 C-terminal amino acids. The necrotic response showed hallmark characteristics of a genuine hypersensitive reaction, such as the accumulation of phytoalexins, reactive oxygen species, pathogenesis-related protein 1c and hypersensitivity-related (hsr) 203J transcripts. Transient expression of the GFLV-F13 protein 2A(HP) fused to an enhanced green fluorescent protein (EGFP) tag in N.occidentalis by agroinfiltration was sufficient to elicit a hypersensitive reaction. In addition, the GFLV-F13 avirulence factor, when introduced in GFLV-GHu, which causes a compatible reaction on N.occidentalis, elicited necrosis and partially restricted the virus. This is the first identification of a nepovirus avirulence factor that is responsible for a hypersensitive reaction in both the context of virus infection and transient expression.

Published
2017
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3. Nanobody-mediated resistance to Grapevine fanleaf virus in plants

Author

Hemmer, Caroline, primary, Djennane, Samia, additional, Ackerer, Léa, additional, Hleibieh, Kamal, additional, Marmonier, Aurélie, additional, Gersch, Sophie, additional, Garcia, Shahinez, additional, Vigne, Emmanuelle, additional, Komar, Véronique, additional, Perrin, Mireille, additional, Gertz, Claude, additional, Belval, Lorène, additional, Berthold, François, additional, Monsion, Baptiste, additional, Schmitt-Keichinger, Corinne, additional, Lemaire, Olivier, additional, Lorber, Bernard, additional, Gutiérrez, Carlos, additional, Muyldermans, Serge, additional, Demangeat, Gérard, additional, and Ritzenthaler, Christophe, additional

Published
2017
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4. Structural basis of Nanobody-mediated plant virus resistance and vector transmission revealed by cryo-EM

Author

Hemmer, Caroline, primary, Orlov, Igor, additional, Ackerer, Léa, additional, Marmonier, Aurélie, additional, Hleibieh, Kamal, additional, Schmitt-Keichinger, Corinne, additional, Vigne, Emmanuelle, additional, Gersch, Sophie, additional, Komar, Véronique, additional, Belval, Lorène, additional, Berthold, François, additional, Monsion, Baptiste, additional, Bron, Patrick, additional, Lemaire, Olivier, additional, Lorber, Bernard, additional, Gutiérrez, Carlos, additional, Muyldermans, Serge, additional, Demangeat, Gérard, additional, Klaholz, Bruno, additional, and Ritzenthaler, Christophe, additional

Published
2016
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5. Display of whole proteins on inner and outer surfaces of grapevine fanleaf virus‐like particles

Author

Belval, Lorène, primary, Hemmer, Caroline, additional, Sauter, Claude, additional, Reinbold, Catherine, additional, Fauny, Jean‐Daniel, additional, Berthold, François, additional, Ackerer, Léa, additional, Schmitt‐Keichinger, Corinne, additional, Lemaire, Olivier, additional, Demangeat, Gérard, additional, and Ritzenthaler, Christophe, additional

Published
2016
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6. Étude du mouvement de cellule à cellule du Grapevine fanleaf virus

Author

BELVAL, Lorène, Hemmer, Caroline, Berthold, François, Marmonier, Aurélie, Komar, Veronique, Gersch, Sophie, Vigne, Emmanuelle, Keichinger, Corinne, Lemaire, Olivier, Ritzenthaler, Christophe, Demangeat, Gerard, Santé de la vigne et qualité du vin (SVQV), Institut National de la Recherche Agronomique (INRA)-Université Louis Pasteur - Strasbourg I, Institut de biologie moléculaire des plantes (IBMP), and Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)

Subjects
[SDV]Life Sciences [q-bio], [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2013

7. Nouvelle approche de visualisation d’un virus dans son hôte : du chameau au virus du court-noué de la vigne

Author

Hemmer, Caroline, Hleibieh, Kamal, Berthold, François, Schmitt-Keichinger, Corinne, Ackerer, Léa, BELVAL, Lorène, Link, Peggy, Marmonier, Aurélie, Komar, Veronique, Muyldermans, Serge, Demangeat, Gerard, Ritzenthaler, Christophe, Santé de la vigne et qualité du vin (SVQV), Institut National de la Recherche Agronomique (INRA)-Université Louis Pasteur - Strasbourg I, Institut de biologie moléculaire des plantes (IBMP), Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA), and Vrije Universiteit Brussel (VUB)

Subjects
[SDV]Life Sciences [q-bio], [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2013

8. Insights into early events in GFLV transmission by the dagger nematode Xiphinema index

Author

Hliebieh, Kamal, Link, Peggy, Hemmer, Caroline, Daujat, Magali, Mutterer, Jérome, Ehrardt, M., Wagner, Richard, Schmitt-Keichinger, Corinne, Berthold, François, Ackerer, Léa, Komar, Veronique, Vigne, Emmanuelle, Marmonier, Aurélie, Gersch, Sophie, Gertz, Claude, Misbach, Jacky, Lemaire, Olivier, Demangeat, Gerard, Ritzenthaler, Christophe, Institut de biologie moléculaire des plantes (IBMP), Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA), Santé de la vigne et qualité du vin (SVQV), Institut National de la Recherche Agronomique (INRA)-Université Louis Pasteur - Strasbourg I, Service d'expérimentation agronomique et viticole (COLM AGRO VITI UE), and Institut National de la Recherche Agronomique (INRA)

Subjects
[SDV]Life Sciences [q-bio], [SDV.BV]Life Sciences [q-bio]/Vegetal Biology
Published
2013

9. Biosensors as novel tools to study Grapevine fanleaf virus movement and transmission

Author

Hemmer, Caroline, Hliebieh, Kamal, Berthold, François, Schmitt-Keichinger, Corinne, Ackerer, Léa, BELVAL, Lorène, Komar, Veronique, Gersch, Sophie, Marmonier, Aurélie, Lemaire, Olivier, Muyldermans, Serge, Demangeat, Gerard, Ritzenthaler, Christophe, Santé de la vigne et qualité du vin (SVQV), Institut National de la Recherche Agronomique (INRA)-Université Louis Pasteur - Strasbourg I, Institut de biologie moléculaire des plantes (IBMP), Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA), and Vrije University Brussel

Subjects
[SDV]Life Sciences [q-bio], [SDV.BV]Life Sciences [q-bio]/Vegetal Biology
Published
2013

10. Viral determinant of the Grapevine fanleaf virus capsid protein involved in virus movement in planta

Author

BELVAL, Lorène, Keichinger, Corinne, Vigne, Emmanuelle, Berthold, François, Marmonier, Aurélie, Lemaire, Olivier, Ritzenthaler, Christophe, Demangeat, Gerard, Santé de la vigne et qualité du vin (SVQV), Institut National de la Recherche Agronomique (INRA)-Université Louis Pasteur - Strasbourg I, Institut de biologie moléculaire des plantes (IBMP), and Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)

Subjects
[SDV]Life Sciences [q-bio], [SDV.BV]Life Sciences [q-bio]/Vegetal Biology
Published
2013

11. Le virus du court noué de la vigne comme vecteur d'expression ou d'extinction de gènes en plante

Author

Berthold, François, Gottula, John, Vigne, Emmanuelle, Fuchs, Marc, Ritzenthaler, Christophe, Schmitt-Keichinger, Corinne, Institut de biologie moléculaire des plantes (IBMP), Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA), Cornell University [New York], Santé de la vigne et qualité du vin (SVQV), and Institut National de la Recherche Agronomique (INRA)-Université Louis Pasteur - Strasbourg I

Subjects
[SDV]Life Sciences [q-bio], [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2013

12. Caractérisation des événements précoces de la transmission du Grapevine fanleaf virus par le nématode Xiphinema index

Author

Hleibieh, Kamal, Link, Peggy, Hemmer, Caroline, Daujat, Magali, Mutterer, Jérome, Erhardt, Mathieu, Wagner, Richard, Schmitt-Keichinger, Corinne, Berthold, François, Ackerer, Léa, Komar, Veronique, Vigne, Emmanuelle, Marmonier, Aurélie, Gersch, Sophie, Gertz, Claude, Misbach, Jacky, Demangeat, Gerard, Ritzenthaler, Christophe, Institut de biologie moléculaire des plantes (IBMP), Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA), Santé de la vigne et qualité du vin (SVQV), Institut National de la Recherche Agronomique (INRA)-Université Louis Pasteur - Strasbourg I, Service d'expérimentation agronomique et viticole (COLM AGRO VITI UE), and Institut National de la Recherche Agronomique (INRA)

Subjects
[SDV]Life Sciences [q-bio], [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2013

13. The 50 distal amino acids of the 2AHP homing protein of <italic>Grapevine fanleaf virus</italic> elicit a hypersensitive reaction on <italic>Nicotiana occidentalis</italic>.

Author

Martin, Isabelle R., Vigne, Emmanuelle, Berthold, François, Komar, Véronique, Lemaire, Olivier, Fuchs, Marc, and Schmitt‐Keichinger, Corinne

Subjects
AMINO acids, GRAPEVINE fanleaf virus, MICROBIAL virulence, HOST-virus relationships, GENE expression in plants, NICOTIANA
Abstract

Summary: Avirulence factors are critical for the arm's race between a virus and its host in determining incompatible reactions. The response of plants to viruses from the genus Nepovirus in the family Secoviridae, including Grapevine fanleaf virus (GFLV), is well characterized, although the nature and characteristics of the viral avirulence factor remain elusive. By using infectious clones of GFLV strains F13 and GHu in a reverse genetics approach with wild‐type, assortant and chimeric viruses, the determinant of necrotic lesions caused by GFLV‐F13 on inoculated leaves of Nicotiana occidentalis was mapped to the RNA2‐encoded protein 2AHP, particularly to its 50 C‐terminal amino acids. The necrotic response showed hallmark characteristics of a genuine hypersensitive reaction, such as the accumulation of phytoalexins, reactive oxygen species, pathogenesis‐related protein 1c and hypersensitivity‐related (hsr) 203J transcripts. Transient expression of the GFLV‐F13 protein 2AHP fused to an enhanced green fluorescent protein (EGFP) tag in N. occidentalis by agroinfiltration was sufficient to elicit a hypersensitive reaction. In addition, the GFLV‐F13 avirulence factor, when introduced in GFLV‐GHu, which causes a compatible reaction on N. occidentalis, elicited necrosis and partially restricted the virus. This is the first identification of a nepovirus avirulence factor that is responsible for a hypersensitive reaction in both the context of virus infection and transient expression. [ABSTRACT FROM AUTHOR]

Published
2018
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14. Nanobody‐mediated resistance to Grapevine fanleaf virus in plants.

Author

Hemmer, Caroline, Djennane, Samia, Ackerer, Léa, Hleibieh, Kamal, Marmonier, Aurélie, Gersch, Sophie, Garcia, Shahinez, Vigne, Emmanuelle, Komar, Véronique, Perrin, Mireille, Gertz, Claude, Belval, Lorène, Berthold, François, Monsion, Baptiste, Schmitt‐Keichinger, Corinne, Lemaire, Olivier, Lorber, Bernard, Gutiérrez, Carlos, Muyldermans, Serge, and Demangeat, Gérard

Subjects
GRAPEVINE fanleaf virus, CROP yields, PATHOGENIC microorganisms, PLANT viruses, NICOTIANA benthamiana
Abstract

Summary: Since their discovery, single‐domain antigen‐binding fragments of camelid‐derived heavy‐chain‐only antibodies, also known as nanobodies (Nbs), have proven to be of outstanding interest as therapeutics against human diseases and pathogens including viruses, but their use against phytopathogens remains limited. Many plant viruses including Grapevine fanleaf virus (GFLV), a nematode‐transmitted icosahedral virus and causal agent of fanleaf degenerative disease, have worldwide distribution and huge burden on crop yields representing billions of US dollars of losses annually, yet solutions to combat these viruses are often limited or inefficient. Here, we identified a Nb specific to GFLV that confers strong resistance to GFLV upon stable expression in the model plant Nicotiana benthamiana and also in grapevine rootstock, the natural host of the virus. We showed that resistance was effective against a broad range of GFLV isolates independently of the inoculation method including upon nematode transmission but not against its close relative, Arabis mosaic virus. We also demonstrated that virus neutralization occurs at an early step of the virus life cycle, prior to cell‐to‐cell movement. Our findings will not only be instrumental to confer resistance to GFLV in grapevine, but more generally they pave the way for the generation of novel antiviral strategies in plants based on Nbs. [ABSTRACT FROM AUTHOR]

Published
2018
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15. The 50 distal amino acids of the 2A HP homing protein of Grapevine fanleaf virus elicit a hypersensitive reaction on Nicotiana occidentalis.

Author

Martin IR, Vigne E, Berthold F, Komar V, Lemaire O, Fuchs M, and Schmitt-Keichinger C

Subjects
Amino Acids chemistry, Genome, Viral genetics, Nepovirus genetics, Plant Diseases immunology, Plant Diseases microbiology, Viral Proteins chemistry, Amino Acids immunology, Nepovirus immunology, Nepovirus pathogenicity, Nicotiana immunology, Nicotiana virology, Viral Proteins immunology
Abstract

Avirulence factors are critical for the arm's race between a virus and its host in determining incompatible reactions. The response of plants to viruses from the genus Nepovirus in the family Secoviridae, including Grapevine fanleaf virus (GFLV), is well characterized, although the nature and characteristics of the viral avirulence factor remain elusive. By using infectious clones of GFLV strains F13 and GHu in a reverse genetics approach with wild-type, assortant and chimeric viruses, the determinant of necrotic lesions caused by GFLV-F13 on inoculated leaves of Nicotiana occidentalis was mapped to the RNA2-encoded protein 2A HP , particularly to its 50 C-terminal amino acids. The necrotic response showed hallmark characteristics of a genuine hypersensitive reaction, such as the accumulation of phytoalexins, reactive oxygen species, pathogenesis-related protein 1c and hypersensitivity-related (hsr) 203J transcripts. Transient expression of the GFLV-F13 protein 2A HP fused to an enhanced green fluorescent protein (EGFP) tag in N. occidentalis by agroinfiltration was sufficient to elicit a hypersensitive reaction. In addition, the GFLV-F13 avirulence factor, when introduced in GFLV-GHu, which causes a compatible reaction on N. occidentalis, elicited necrosis and partially restricted the virus. This is the first identification of a nepovirus avirulence factor that is responsible for a hypersensitive reaction in both the context of virus infection and transient expression., (© 2017 BSPP AND JOHN WILEY & SONS LTD.)

Published
2018
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