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3. Patient-Reported Outcome Measures in Colorectal Surgery: Construction of Core Measures Using Open-Source Research Method

Author

El-Hussuna A, Rubio-Perez I, Millan M, Pellino G, Negoi I, Gallo G, Shalaby M, Celentano V, Green R, Minaya-Bravo A, Emile S, Smart NJ, Maeda Y, Ivatury SJ, Mackenzie G, Yalçinkaya A, Mellenthin C, N Dudi-Venkata N, Davies J, McNair A, Pata F, Gymoese Berthelsen K, Rivadeneira D, Spinelli A, Myrelid P, Mayol J, Wexner S, and OpenSourceResearch collaboration

Subjects
collaborative, colorectal, inflammatory bowel disease, patient, patient-reported outcome measures, surgery, female genital diseases and pregnancy complications
Abstract

Purpose . The primary aim of the study was to review the existing literature about patient-reported outcome measures (PROMs) in colorectal cancer and IBD. The secondary aim was to present a road map to develop a core outcome set via opinion gathering using social media. Method . This study is the first step of a three-step project aimed at constructing simple, applicable PROMs in colorectal surgery. This article was written in a collaborative manner with authors invited both through Twitter via the #OpenSourceResearch hashtag. The 5 most used PROMs were presented and discussed as slides/images on Twitter. Inputs from a wide spectrum of participants including researchers, surgeons, physicians, nurses, patients, and patients' organizations were collected and analyzed. The final draft was emailed to all contributors and 6 patients' representatives for proofreading and approval. Results . Five PROM sets were identified and discussed: EORTC QLQ-CR29, IBDQ short health questionnaire, EORTC QLQ-C30, ED-Q5-5L, and Short Form-36. There were 315 tweets posted by 50 tweeters with 1458 retweets. Awareness about PROMs was generally limited. The general psycho-physical well-being score (GPP) was suggested and discussed, and then a survey was conducted in which more than 2/3 of voters agreed that GPP covers the most important aspects in PROMs. Conclusion . Despite the limitations of this exploratory study, it offered a new method to conduct clinical research with opportunity to engage patients. The general psycho-physical well-being score suggested as simple, applicable PROMs to be eventually combined procedure-specific, disease-specific, or symptom-specific PROMs if needed.

Published
2021

4. Intraoperative transfusion practices in Europe

Author

Meier, J., Filipescu, D., Kozek Langenecker, S., Llau Pitarch, J., Mallett, S., Martus, P., Matot, I., ETPOS collaborators: Accurso, G., Ahrens, N., Akan, M., Åkeröy, K., Aksoy, O., Alanoğlu, Z., Alfredo, M., Alkis, N., Almeida, V., Alousi, M., Alves, C., Amaral, J., Ambrosi, X., Ana, I., Anastase, D., Andersson, M., Andreou, A., Anthopoulos, G., Apanaviciute, D., Arbelaez, A., Arcade, A., Arion Balescu, C., Arun, O., Azenha, M., Bacalbasa, N., Baeten, W., Balandin, A., Barquero López, M., Barsan, V., Bascuas, B., Basora, M., Baumann, H., Bayer, A., Bell, A., Belmonte Cuenca, J., Bengisun, Z., Bento, C., Beran, M., Bermudez Lopez, M., Bernardino, A., Berthelsen, K., Bigat, Z., Bilshiene, D., Bilska, M., Bisbe Vives, E., Biscioni, T., Björn, H., Blom, T., Bogdan Prodan, A., Bogdanovic Dvorscak, M., Boisson, M., Bolten, J., Bona, F., Borg, F., Boros, C., Borys, M., Boveroux, P., Boztug Uz, N., Brettner, F., Brisard, L., Britta de, W., Browne, G., Budow, K., Buerkle, H., Buggy, D., Cain, A., Calancea, E., Calarasu, F., Calder, V., Camci, A., Campiglia, L., Campos, B., Camps, A., Carlos, D., Carreira, C., Carrilho, A., Carvalho, P., Cassinello, C., Cattan, A., Cenni, L., Cerny, V., Ceyda Meço, B., Chesov, I., Chishti, A., Chupin, A., Cikova, A., Cindea, I., Cintula, D., Ciobanasu, R., Clements, D., Cobiletchi, S., Coburn, M., Coghlan, L., Collyer, T., Copotoiu, S., Copotoiu, R., Corneci, D., CORTEGIANI, Andrea, Coskunfirat, O., Costea, D., Czuczwar, M., Davies, K., De Baerdemaeker, L., De Hert, S., Debernardi, F., Decagny, S., Deger Coskunfirat, N., Diana, T., Diana, G., Dias, S., Dickinson, M., Dobisova, A., Dragan, A., Droc, G., Duarte, S., Dunk, N., Ekelund, K., Ekmekçi, P., Elena, C., Ellimah, T., Espie, L., Everett, L., Ferguson, A., Fernandes, M., Fernández, J., Ferner, M., Ferreira, D., Ferrie, R., Flassikova, Z., Fleischer, A., Font, A., Galkova, K., Garcia, I., Garner, M., Gasenkampf, A., Gelmanas, A., Gherghina, V., Gilsanz, F., Giokas, G., Goebel, U., Gomes, P., Gonçalves Aguiar, J., Gonzalez Monzon, V., Gottschalk, A., Gouraud, J., Gramigni, E., Grintescu, I., Grynyuk, A., Grytsan, A., Guasch, E., Gustin, D., Hans, G., Harazim, H., Hervig, T., Hidalgo, F., Higham, C., Hirschauer, N., Hoeft, A., Innerhofer, P., Innerhofer Pompernigg, N., Jacobs, S., Jakobs, N., Jamaer, L., James, S., Jawad, M., Jesus, J., Jhanji, S., Jipa Lavina, N., Jokinen, J., Jovanovic, G., Jubera, M., Kahn, D., Karjagin, J., Kasnik, D., Katsanoulas, K., Kelle, H., Kelleher, M., Kessler, F., Kirigin, B., Kiskira, O., Kivik, P., Klimi, P., Klučka, J., Koers, L., Kontrimaviciut, E., Koopman van Gemert, A., Korfiotis, D., Kosinová, M., Koursoumi, E., Kranke, P., Kresic, M., Krobot, R., Kropman, L., Kulikov, A., Kvolik, S., Kvrgic, I., Kyttari, A., Lagarto, F., Lance, M., Laufenberg, R., Lauwick, S., Lecoq, J., Leech, L., Lidzborski, L., Liliana, H., Linda, F., Lopes, A., Lopez, L., Lopez Alvarez, A., Lorenzi, I., Lorre, G., Lucian, H., Lupis, T., Lupu, M., Macas, A., Macedo, A., Maggi, G., Mallor, T., Manoleli, A., Manolescu, R., Manrique, S., Maquoi, I., Marios Konstantinos, T., Markovic Bozic, J., Markus, W., Marques, M., Martinez, R., Martinez, E., Martínez, E., Martinho, H., Martins, D., Martires, E., Matias, F., Mauff, S., Meale, P., Merz, H., Meybohm, P., Militello, M., Mincu, N., Miranda, M., Mirea, L., Moghildea, V., Moise, A., Molano Diaz, P., Moltó, L., Monedero, P., Moral, V., Moreira, Z., Moret, E., Mulders, F., Munteanu, A., Nadia Diana, K., Nair, A., Neskovic, V., Ninane, V., Nitu, D., Oberhofer, D., Odeberg Wernerman, S., Oganjan, J., Omur, D., Orallo Moran, M., Ozkardesler, S., Pacasová, R., Paklar, N., Pandazi, A., Papaspyros, F., Paraskeuopoulos, T., Parente, S., Paunescu, M., Pavičić Šarić, J., Pereira, F., Pereira, E., Pereira, L., Perry, C., Petri, A., Petrovic, U., Pica, S., Pinheiro, F., Pinto, J., Pinto, F., Piwowarczyk, P., Platteau, S., Poeira, R., Popescu, R., Popica, G., Poredos, P., Prasser, C., Preckel, B., Prospiech, A., Pujol, R., Raimundo, A., RAINERI, Santi Maurizio, Rakic, D., Ramadan, M., Ramazanoğlu, A., Rantis, A., Raquel, F., Rätsep, I., Real, C., Reikvam, T., Reis, L., Rigal, J., Rohner, A., Rokk, A., Roman Fernandez, A., Rosenberger, P., Rossaint, R., Rozec, B., Rudolph, T., Saeed, Y., Safonov, S., Saka, E., Samama, C., Sánchez López, Ó., Sanchez Perez, D., Sanchez Sanchez, Y., Sandeep, V., Sandu, M., Sanlı, S., Saraiva, A., Scarlatescu, E., Schiraldi, R., Schittek, G., Schnitter, B., Schuster, M., Seco, C., Selvi, O., Senard, M., Serra, S., Serrano, H., Shmigelsky, A., Silva, L., Simeson, K., Singh, R., Sipylaite, J., Skitek, K., Skok, I., Smékalová, O., Smirnova, N., Sofia, M., Soler Pedrola, M., Söndergaard, S., Sõrmus, A., Sørvoll, I., Soumelidis, C., Spindler Yesel, A., Stefan, M., Stevanovic, A., Stevikova, J., Stivan, S., Štourač, P., Striteska, J., Strys, L., Suljevic, I., Tania, M., Tareco, G., Tena, B., Theodoraki, K., Tifrea, M., Tikuisis, R., Tolós, R., Tomasi, R., Tomescu, D., Tomkute, G., Tormos, P., Trepenaitis, D., Troyan, G., Unic Stojanovic, D., Unterrainer, A., Uranjek, J., Valsamidis, D., van Dasselaar, N., Van Limmen, J., van Noord, P., van Poorten, J., Vanderlaenen, M., Varela Garcia, O., Velasco, A., Veljovic, M., Vera Bella, J., Vercauteren, M., Verdouw, B., Verenkin, V., Veselovsky, T., Vieira, H., Villar, T., Visnja, I., Voje, M., von Dossow Hanfstingl, V., Von Langen, D., Vorotyntsev, S., Vujanovič, V., Vukovic, R., Watt, P., Werner, E., Wernerman, J., Wittmann, M., Wright, M., Wunder, C., Wyffels, P., Yakymenko, Y., Yıldırım, Ç., Yılmaz, H., Zacharowski, K., Záhorec, R., Zarif, M., Zielinska Skitek, E., Zsisku, L., Selçuk Üniversitesi, Meier, J., Filipescu, D., Kozek-Langenecker, S., Llau Pitarch, J., Mallett, S., Martus, P., Matot, I., ETPOS collaborators: Accurso, G., Ahrens, N., Akan, M., Åkeröy, K., Aksoy, O., Alanoğlu, Z., Alfredo, M., Alkis, N., Almeida, V., Alousi, M., Alves, C., Amaral, J., Ambrosi, X., Ana, I., Anastase, D., Andersson, M., Andreou, A., Anthopoulos, G., Apanaviciute, D., Arbelaez, A., Arcade, A., Arion-Balescu, C., Arun, O., Azenha, M., Bacalbasa, N., Baeten, W., Balandin, A., Barquero López, M., Barsan, V., Bascuas, B., Basora, M., Baumann, H., Bayer, A., Bell, A., Belmonte Cuenca, J., Bengisun, Z., Bento, C., Beran, M., Bermudez Lopez, M., Bernardino, A., Berthelsen, K., Bigat, Z., Bilshiene, D., Bilska, M., Bisbe Vives, E., Biscioni, T., Björn, H., Blom, T., Bogdan Prodan, A., Bogdanovic Dvorscak, M., Boisson, M., Bolten, J., Bona, F., Borg, F., Boros, C., Borys, M., Boveroux, P., Boztug Uz, N., Brettner, F., Brisard, L., Britta de, W., Browne, G., Budow, K., Buerkle, H., Buggy, D., Cain, A., Calancea, E., Calarasu, F., Calder, V., Camci, A., Campiglia, L., Campos, B., Camps, A., Carlos, D., Carreira, C., Carrilho, A., Carvalho, P., Cassinello, C., Cattan, A., Cenni, L., Cerny, V., Ceyda Meço, B., Chesov, I., Chishti, A., Chupin, A., Cikova, A., Cindea, I., Cintula, D., Ciobanasu, R., Clements, D., Cobiletchi, S., Coburn, M., Coghlan, L., Collyer, T., Copotoiu, S., Copotoiu, R., Corneci, D., Cortegiani, A., Coskunfirat, O., Costea, D., Czuczwar, M., Davies, K., De Baerdemaeker, L., De Hert, S., Debernardi, F., Decagny, S., Deger Coskunfirat, N., Diana, T., Diana, G., Dias, S., Dickinson, M., Dobisova, A., Dragan, A., Droc, G., Duarte, S., Dunk, N., Ekelund, K., Ekmekçi, P., Elena, C., Ellimah, T., Espie, L., Everett, L., Ferguson, A., Fernandes, M., Fernández, J., Ferner, M., Ferreira, D., Ferrie, R., Flassikova, Z., Fleischer, A., Font, A., Galkova, K., Garcia, I., Garner, M., Gasenkampf, A., Gelmanas, A., Gherghina, V., Gilsanz, F., Giokas, G., Goebel, U., Gomes, P., Gonçalves Aguiar, J., Gonzalez Monzon, V., Gottschalk, A., Gouraud, J., Gramigni, E., Grintescu, I., Grynyuk, A., Grytsan, A., Guasch, E., Gustin, D., Hans, G., Harazim, H., Hervig, T., Hidalgo, F., Higham, C., Hirschauer, N., Hoeft, A., Innerhofer, P., Innerhofer-Pompernigg, N., Jacobs, S., Jakobs, N., Jamaer, L., James, S., Jawad, M., Jesus, J., Jhanji, S., Jipa Lavina, N., Jokinen, J., Jovanovic, G., Jubera, M., Kahn, D., Karjagin, J., Kasnik, D., Katsanoulas, K., Kelle, H., Kelleher, M., Kessler, F., Kirigin, B., Kiskira, O., Kivik, P., Klimi, P., Klučka, J., Koers, L., Kontrimaviciut, E., Koopman-van Gemert, A., Korfiotis, D., Kosinová, M., Koursoumi, E., Kozek Langenecker, S., Kranke, P., Kresic, M., Krobot, R., Kropman, L., Kulikov, A., Kvolik, S., Kvrgic, I., Kyttari, A., Lagarto, F., Lance, M., Laufenberg, R., Lauwick, S., Lecoq, J., Leech, L., Lidzborski, L., Liliana, H., Linda, F., Lopes, A., Lopez, L., Lopez Alvarez, A., Lorenzi, I., Lorre, G., Lucian, H., Lupis, T., Lupu, M., Macas, A., Macedo, A., Maggi, G., Mallor, T., Manoleli, A., Manolescu, R., Manrique, S., Maquoi, I., Marios-Konstantinos, T., Markovic Bozic, J., Markus, W., Marques, M., Martinez, R., Martinez, E., Martínez, E., Martinho, H., Martins, D., Martires, E., Matias, F., Mauff, S., Meale, P., Merz, H., Meybohm, P., Militello, M., Mincu, N., Miranda, M., Mirea, L., Moghildea, V., Moise, A., Molano Diaz, P., Moltó, L., Monedero, P., Moral, V., Moreira, Z., Moret, E., Mulders, F., Munteanu, A., Nadia Diana, K., Nair, A., Neskovic, V., Ninane, V., Nitu, D., Oberhofer, D., Odeberg-Wernerman, S., Oganjan, J., Omur, D., Orallo Moran, M., Ozkardesler, S., Pacasová, R., Paklar, N., Pandazi, A., Papaspyros, F., Paraskeuopoulos, T., Parente, S., Paunescu, M., Pavičić Šarić, J., Pereira, F., Pereira, E., Pereira, L., Perry, C., Petri, A., Petrovic, U., Pica, S., Pinheiro, F., Pinto, J., Pinto, F., Piwowarczyk, P., Platteau, S., Poeira, R., Popescu, R., Popica, G., Poredos, P., Prasser, C., Preckel, B., Prospiech, A., Pujol, R., Raimundo, A., Raineri, S., Rakic, D., Ramadan, M., Ramazanoğlu, A., Rantis, A., Raquel, F., Rätsep, I., Real, C., Reikvam, T., Reis, L., Rigal, J., Rohner, A., Rokk, A., Roman Fernandez, A., Rosenberger, P., Rossaint, R., Rozec, B., Rudolph, T., Saeed, Y., Safonov, S., Saka, E., Samama, C., Sánchez López, Ó., Sanchez Perez, D., Sanchez Sanchez, Y., Sandeep, V., Sandu, M., Sanlı, S., Saraiva, A., Scarlatescu, E., Schiraldi, R., Schittek, G., Schnitter, B., Schuster, M., Seco, C., Selvi, O., Senard, M., Serra, S., Serrano, H., Shmigelsky, A., Silva, L., Simeson, K., Singh, R., Sipylaite, J., Skitek, K., Skok, I., Smékalová, O., Smirnova, N., Sofia, M., Soler Pedrola, M., Söndergaard, S., Sõrmus, A., Sørvoll, I., Soumelidis, C., Spindler Yesel, A., Stefan, M., Stevanovic, A., Stevikova, J., Stivan, S., Štourač, P., Striteska, J., Strys, L., Suljevic, I., Tania, M., Tareco, G., Tena, B., Theodoraki, K., Tifrea, M., Tikuisis, R., Tolós, R., Tomasi, R., Tomescu, D., Tomkute, G., Tormos, P., Trepenaitis, D., Troyan, G., Unic-Stojanovic, D., Unterrainer, A., Uranjek, J., Valsamidis, D., van Dasselaar, N., Van Limmen, J., van Noord, P., van Poorten, J., Vanderlaenen, M., Varela Garcia, O., Velasco, A., Veljovic, M., Vera Bella, J., Vercauteren, M., Verdouw, B., Verenkin, V., Veselovsky, T., Vieira, H., Villar, T., Visnja, I., Voje, M., von Dossow-Hanfstingl, V., Von Langen, D., Vorotyntsev, S., Vujanovič, V., Vukovic, R., Watt, P., Werner, E., Wernerman, J., Wittmann, M., Wright, M., Wunder, C., Wyffels, P., Yakymenko, Y., Yıldırım, Ç., Yılmaz, H., Zacharowski, K., Záhorec, R., Zarif, M., Zielinska-Skitek, E., Zsisku, L., Anesthesiology, Graduate School, ACS - Amsterdam Cardiovascular Sciences, and AII - Amsterdam institute for Infection and Immunity

Subjects
AUSTRIAN BENCHMARK, Male, Blood transfusion, medicine.medical_treatment, 610 Medizin, anaemia, anesthesia, blood transfusion, surgery, transfusion trigger, 030204 cardiovascular system & hematology, GUIDELINES, surgery, Cohort Studies, 0302 clinical medicine, 030202 anesthesiology, Medicine and Health Sciences, Medicine, Prospective Studies, Prospective cohort study, ddc:610, Research Support, Non-U.S. Gov't, Middle Aged, Hospitals, Europe, Female, Allogeneic transfusion, Cohort study, medicine.medical_specialty, Transfusion rate, Observational Study, anesthesia, blood transfusion, ELECTIVE SURGERY, Clinical Practice, 03 medical and health sciences, Journal Article, anaemia, transfusion trigger, Humans, Blood Transfusion, Elective surgery, CHLC ANS, Intensive care medicine, Intraoperative Care, business.industry, PREOPERATIVE ANEMIA, PATIENT BLOOD MANAGEMENT, Clinical trial, Anesthesiology and Pain Medicine, Emergency medicine, business, Packed red blood cells, REQUIREMENTS
Abstract

PubMed: 26787795, Background: Transfusion of allogeneic blood influences outcome after surgery. Despite widespread availability of transfusion guidelines, transfusion practices might vary among physicians, departments, hospitals and countries. Our aim was to determine the amount of packed red blood cells (pRBC) and blood products transfused intraoperatively, and to describe factors determining transfusion throughout Europe. Methods: We did a prospective observational cohort study enrolling 5803 patients in 126 European centres that received at least one pRBC unit intraoperatively, during a continuous three month period in 2013. Results: The overall intraoperative transfusion rate was 1.8%; 59% of transfusions were at least partially initiated as a result of a physiological transfusion trigger- mostly because of hypotension (55.4%) and/or tachycardia (30.7%). Haemoglobin (Hb)- based transfusion trigger alone initiated only 8.5% of transfusions. The Hb concentration [mean (sd)] just before transfusion was 8.1 (1.7) g dl-1 and increased to 9.8 (1.8) g dl-1 after transfusion. The mean number of intraoperatively transfused pRBC units was 2.5 (2.7) units (median 2). Conclusions: Although European Society of Anaesthesiology transfusion guidelines are moderately implemented in Europe with respect to Hb threshold for transfusion (7-9 g dl-1), there is still an urgent need for further educational efforts that focus on the number of pRBC units to be transfused at this threshold. © 2016 The Author. Published by Oxford University Press on behalf of the British Journal of Anaesthesia.

Published
2016

6. An Efficient Markov Chain Monte Carlo Method for Distributions With Intractable Normalising Constants

Author

Moller, J, Pettitt, Anthony, Reeves, Robert, Berthelsen, K, Moller, J, Pettitt, Anthony, Reeves, Robert, and Berthelsen, K

Abstract

Maximum likelihood parameter estimation and sampling from Bayesian posterior distributions are problematic when the probability density for the parameter of interest involves an intractable normalising constant which is also a function of that parameter. In this paper, an auxiliary variable method is presented which requires only that independent samples can be drawn from the unnormalised density at any particular parameter value. The proposal distribution is constructed so that the normalising constant cancels from the Metropolis-Hastings ratio. The method is illustrated by producing posterior samples for parameters of the Ising model given a particular lattice realisation.

Published
2006

7. An efficient Markov chain Monte Carlo method for distributions with intractable normalising constants.

Author

Pettitt, Anthony, Berthelsen, K., Moller, J., Reeves, R., Pettitt, Anthony, Berthelsen, K., Moller, J., and Reeves, R.

Abstract

Maximum likelihood parameter estimation and sampling from Bayesian posterior distributions are problematic when the probability density for the parameter of interest involves an intractable normalising constant which is also a function of that parameter. In this paper, an auxiliary variable method is presented which requires only that independent samples can be drawn from the unnormalised density at any particular parameter value. The proposal distribution is constructed so that the normalising constant cancels from the Metropolis-Hastings ratio. The method is illustrated by producing posterior samples for parameters of the Ising model given a particular lattice realisation.

Published
2006

8. Breakdown tests of glass fibre reinforced polymers (GFRP) as part of improved lightning protection of wind turbine blades

Author

Madsen, Søren Find, Holbøll, Joachim, Henriksen, Mogens, Larsen, F M, Hansen, L B, Berthelsen, K, Madsen, Søren Find, Holbøll, Joachim, Henriksen, Mogens, Larsen, F M, Hansen, L B, and Berthelsen, K

Abstract

This paper addresses a need for analysing the interaction between electrical discharges and GFRP. A test method for evaluating the breakdown and withstand voltages for materials used in wind turbine blades has been developed. The method is based on IEC 243-3, methods of test for electrical strength of solid insulating materials, and simulates the situation in a wind turbine blade, where a lightning discharge penetrates an insulating layer towards an inner earth conductor. Different GFRP materials supplied by Danish wind turbine blade manufacturers have been tested. In the subsequent experiments, both the test methods and the results are evaluated. The different results are compared with respect to the GFRP materials' overall thicknesses, thickness of laminates and thickness of coatings. Finally, some general conclusions and different statements on how to manufacture specimens with minimum likelihood of breakdown are stated.

Published
2004

10. Nutrient intake and health status of vegans. Chemical analyses of diets using the duplicate portion sampling technique

Author

Abdulla, M, primary, Andersson, I, additional, Asp, N G, additional, Berthelsen, K, additional, Birkhed, D, additional, Dencker, I, additional, Johansson, C G, additional, Jägerstad, M, additional, Kolar, K, additional, Nair, B M, additional, Nilsson-Ehle, P, additional, Nordén, A, additional, Rassner, S, additional, Akesson, B, additional, and Ockerman, P A, additional

Published
1981
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12. Xanthoria parietina as a monitor of ground-level ambient ammonia concentrations.

Author

Olsen HB, Berthelsen K, Andersen HV, and Søchting U

Subjects
Analysis of Variance, Denmark, Environmental Monitoring statistics & numerical data, Air Pollutants analysis, Ammonia analysis, Ascomycota chemistry, Environmental Monitoring methods, Lichens chemistry, Nitrogen analysis
Abstract

Total nitrogen in transplanted and in situ lichen thalli of Xanthoria parietina were related to ambient ammonia air concentrations measured with passive ALPHA (Adapted Low-cost Passive High Absorption) diffusion samplers in Denmark. Transplants and ALPHA samplers were exposed four months in a transect on heathland close to a pig farm. Monthly mean ammonia concentrations declined exponentially approaching background levels after 300m. Nitrogen content of the lichen transplants tended to decline with distance, though only a few stations were significantly different from each other. Where ammonia concentrations were high, maximum content of nitrogen was reached after one month of exposure. Conversely, at sites with background concentrations, it took several months to reach a statistically significant uptake. The correlation between ammonia concentration in the air and in situ X. parietina was significant., (Copyright (c) 2009 Elsevier Ltd. All rights reserved.)

Published
2010
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13. Phylogenetic relationships of Triticum and Aegilops and evidence for the origin of the A, B, and D genomes of common wheat (Triticum aestivum).

Author

Petersen G, Seberg O, Yde M, and Berthelsen K

Subjects
Evolution, Molecular, Poaceae genetics, Triticum genetics, Genome, Plant, Phylogeny, Poaceae classification, Triticum classification
Abstract

Common wheat (Triticum aestivum) has for decades been a textbook example of the evolution of a major crop species by allopolyploidization. Using a sophisticated extension of the PCR technique, we have successfully isolated two single-copy nuclear genes, DMC1 and EF-G, from each of the three genomes found in hexaploid wheat (BA(u)D) and from the two genomes of the tetraploid progenitor Triticum turgidum (BA(u)). By subjecting these sequences to phylogenetic analysis together with sequences from representatives of all the diploid Triticeae genera we are able for the first time to provide simultaneous and strongly supported evidence for the D genome being derived from Aegilops tauschii, the A(u) genome being derived from Triticum urartu, and the hitherto enigmatic B genome being derived from Aegilops speltoides. Previous problems of identifying the B genome donor may be associated with a higher diversification rate of the B genome compared to the A(u) genome in the polyploid wheats. The phylogenetic hypothesis further suggests that neither Triticum, Aegilops, nor Triticum plus Aegilops are monophyletic.

Published
2006
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