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Your search keyword '"Berrang, Mark E."' showing total 257 results
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257 results on '"Berrang, Mark E."'

4. Susceptibility of pESI positive Salmonella to treatment with biocide chemicals approved for use in poultry meat processing as compared to Salmonella without the pESI plasmid.

Author

McMillan, Elizabeth A, Adams, Eric S, Mitchell, Trevor R, Hawkins, Jaci A, Read, Quentin D, Pokoo-Aikins, Anthony, Berrang, Mark E, Harris, Caitlin E, Hughes, Michael D, Glenn, Anthony E, and Meinersmann, Richard J

Subjects
POULTRY as food, CHEMICAL processes, POULTRY products, FOODBORNE diseases, POULTRY processing
Abstract

Salmonella is a common cause of human foodborne illness, which is frequently associated with consumption of contaminated or undercooked poultry meat. Serotype Infantis is among the most common serotypes isolated from poultry meat products globally. Isolates of serotype Infantis carrying the pESI plasmid, the most dominant strain of Infantis, have been shown to exhibit oxidizer tolerance. Therefore, 16 strains of Salmonella with and without pESI carriage were investigated for susceptibility to biocide chemical processing aids approved for use in US poultry meat processing: peracetic acid (PAA), cetylpyridinium chloride (CPC), calcium hypochlorite, and sodium hypochlorite. Strains were exposed for 15 s to simulate spray application and 90 min to simulate application in an immersion chiller. All strains tested were susceptible to all concentrations of PAA, CPC, and sodium hypochlorite when applied for 90 min. When CPC, calcium hypochlorite, and sodium hypochlorite were applied for 15 s to simulate spray time, strains responded similarly to each other. However, strains responded variably to exposure to PAA. The variation was not statistically significant and appears unrelated to pESI carriage. Results highlight the necessity of testing biocide susceptibility in the presence of organic material and in relevant in situ applications. [ABSTRACT FROM AUTHOR]

Published
2024
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18. Whole-genome sequencing of Listeria innocua recovered from retail milk and dairy products in Egypt

Author

Ramadan, Hazem, Al-Ashmawy, Maha, Soliman, Ahmed M., Elbediwi, Mohammed, Sabeq, Islam, Yousef, Mona, Algammal, Abdelazeem M., Hiott, Lari M., Berrang, Mark E., Frye, Jonathan G., and Jackson, Charlene R.

Subjects
Microbiology (medical), genetic context, clpL gene, milk, whole-genome sequencing, phylogenetic analysis, Egypt, 500 Naturwissenschaften und Mathematik::570 Biowissenschaften, Biologie::570 Biowissenschaften, Biologie, Listeria innocua, Microbiology
Abstract

The similarity of the Listeria innocua genome with Listeria monocytogenes and their presence in the same niche may facilitate gene transfer between them. A better understanding of the mechanisms responsible for bacterial virulence requires an in-depth knowledge of the genetic characteristics of these bacteria. In this context, draft whole genome sequences were completed on five L. innocua isolated from milk and dairy products in Egypt. The assembled sequences were screened for antimicrobial resistance and virulence genes, plasmid replicons and multilocus sequence types (MLST); phylogenetic analysis of the sequenced isolates was also performed. The sequencing results revealed the presence of only one antimicrobial resistance gene, fosX, in the L. innocua isolates. However, the five isolates carried 13 virulence genes involved in adhesion, invasion, surface protein anchoring, peptidoglycan degradation, intracellular survival, and heat stress; all five lacked the Listeria Pathogenicity Island 1 (LIPI-1) genes. MLST assigned these five isolates into the same sequence type (ST), ST-1085; however, single nucleotide polymorphism (SNP)-based phylogenetic analysis revealed 422–1,091 SNP differences between our isolates and global lineages of L. innocua. The five isolates possessed an ATP-dependent protease (clpL) gene, which mediates heat resistance, on a rep25 type plasmids. Blast analysis of clpL-carrying plasmid contigs showed approximately 99% sequence similarity to the corresponding parts of plasmids of L. monocytogenes strains 2015TE24968 and N1-011A previously isolated from Italy and the United States, respectively. Although this plasmid has been linked to L. monocytogenes that was responsible for a serious outbreak, this is the first report of L. innocua containing clpL-carrying plasmids. Various genetic mechanisms of virulence transfer among Listeria species and other genera could raise the possibility of the evolution of virulent strains of L. innocua. Such strains could challenge processing and preservation protocols and pose health risks from dairy products. Ongoing genomic research is necessary to identify these alarming genetic changes and develop preventive and control measures.

Published
2023
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20. Survey of Meat Collected from Commercial Broiler Processing Plants Suggests Low Levels of Semicarbazide Can Be Created during Immersion Chilling

Author

Mitchell, Trevor R., primary, Berrang, Mark E., additional, Gold, Scott E., additional, Lawrence, Kurt C., additional, Glenn, Anthony E., additional, Gamble, Gary R., additional, Feldner, Peggy W., additional, Hawkins, Jaci A., additional, Miller, Christine E., additional, Olson, Drew E., additional, Chatterjee, Debolina, additional, Mcdonough, Callie M., additional, and Pokoo-Aikins, Anthony, additional

Published
2022
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23. Erratum for Oladeinde et al., “Horizontal Gene Transfer Is the Main Driver of Antimicrobial Resistance in Broiler Chicks Infected with Salmonella enterica Serovar Heidelberg”

Author

Oladeinde, Adelumola, primary, Abdo, Zaid, additional, Press, Maximilian O., additional, Cook, Kimberly, additional, Cox, Nelson A., additional, Zwirzitz, Benjamin, additional, Woyda, Reed, additional, Lakin, Steven M., additional, Thomas, Jesse C., additional, Looft, Torey, additional, Cosby, Douglas E., additional, Hinton, Arthur, additional, Guard, Jean, additional, Line, Eric, additional, Rothrock, Michael J., additional, Berrang, Mark E., additional, Herrington, Kyler, additional, Zock, Gregory, additional, Plumblee Lawrence, Jodie, additional, Cudnik, Denice, additional, House, Sandra, additional, Ingram, Kimberly, additional, Lariscy, Leah, additional, Wagner, Martin, additional, Aggrey, Samuel E., additional, Chai, Lilong, additional, and Ritz, Casey, additional

Published
2021
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25. Horizontal Gene Transfer Is the Main Driver of Antimicrobial Resistance in Broiler Chicks Infected with Salmonella enterica Serovar Heidelberg

Author

Oladeinde, Adelumola, primary, Abdo, Zaid, additional, Press, Maximilian O., additional, Cook, Kimberly, additional, Cox, Nelson A., additional, Zwirzitz, Benjamin, additional, Woyda, Reed, additional, Lakin, Steven M., additional, Thomas, Jesse C., additional, Looft, Torey, additional, Cosby, Douglas E., additional, Hinton, Arthur, additional, Guard, Jean, additional, Line, Eric, additional, Rothrock, Michael J., additional, Berrang, Mark E., additional, Herrington, Kyler, additional, Zock, Gregory, additional, Plumblee Lawrence, Jodie, additional, Cudnik, Denice, additional, House, Sandra, additional, Ingram, Kimberly, additional, Lariscy, Leah, additional, Wagner, Martin, additional, Aggrey, Samuel E., additional, Chai, Lilong, additional, and Ritz, Casey, additional

Published
2021
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27. Gene flux and acid-imposed selection are the main drivers of antimicrobial resistance in broiler chicks infected with Salmonella enterica serovar Heidelberg

Author

Oladeinde, Adelumola, primary, Abdo, Zaid, additional, Press, Maximilian O., additional, Cook, Kimberly, additional, Cox, Nelson A., additional, Zwirzitz, Benjamin, additional, Woyda, Reed, additional, Lakin, Steven M., additional, Thomas, Jesse C., additional, Looft, Torey, additional, Cosby, Douglas E., additional, Hinton, Arthur, additional, Guard, Jean, additional, Line, Eric, additional, Rothrock, Michael J., additional, Berrang, Mark E., additional, Herrington, Kyler, additional, Zock, Gregory, additional, Lawrence, Jodie Plumblee, additional, Cudnik, Denice, additional, House, Sandra, additional, Ingram, Kimberly, additional, Lariscy, Leah, additional, Wagner, Martin, additional, Aggrey, Samuel E., additional, Chai, Lilong, additional, and Ritz, Casey, additional

Published
2021
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28. Multilocus sequence typing of Listeria monocytogenes by use of hypervariable genes reveals clonal and recombination histories of three lineages

Author

Meinersmann, Richard J., Phillips, Robert W., Wiedmann, Martin, and Berrang, Mark E.

Subjects
Genetic transformation -- Analysis, Listeria monocytogenes -- Research, Biological sciences
Abstract

Inheritance, mutation and horizontal gene transfer in Listeria monocytogenes and the recombinant construction of mosaic genes in three different patterns for each of the three Listeria monocytogenes lineages is studied using multilocus sequence typing analysis. Results show that the different lineages of Listeria monocytogenes can share habitats but their horizontal gene transfer histories are different.

Published
2004

37. Prevalence and Characterization of Quinolone Resistance in Campylobacter spp. Isolates in Chicken Livers from Retail Stores in Georgia, USA.

Author

HUNG-YUEH YEH, COX, NELSON A., HINTON, JR., ARTHUR, BERRANG, MARK E., LAWRENCE, JODIE R. PLUMBLEE, and THOMPSON, TORI M.

Abstract

Campylobacter is a bacterial pathogen that causes human foodborne illnesses worldwide, and outbreaks have been associated with consumption of undercooked chicken livers. The objectives of this study were to compare two PCR assays of 250 Campylobacter isolates for identification to species, to assess antibiotic resistance of the isolates, and to analyze genetic diversity of the quinolone resistance determining regions (QRDRs) of the isolates. A double-blind design was used to identify the species of Campylobacter; 181 (72%) of the isolates were identified as Campylobacter jejuni, and 69 (28%) isolates were identified as Campylobacter coli by both PCR assays. A total of 93 (37.2%) isolates were resistant to at least one antibiotic. Among 88 C. jejuni isolates, 33 (18%) were resistant to nalidixic acid (NAL) and ciprofloxacin (CIP), 25 (14%) were resistant to tetracycline (TET), and 18 (10%) were resistant to NAL and TET. Two C. jejuni isolates were resistant to four of the tested antibiotics, and one isolate was resistant to five antibiotics. Two C. coli isolates were resistant to TET, and two were resistant to NAL, CIP, and TET. The amino acid sequences of the QRDRs for the isolates had eight point mutations and could be classified into 12 groups. Thirty-eight C. jejuni isolates resistant to NAL and CIP had a point mutation at residue 86 (substitution from threonine to isoleucine). However, six isolates without this substitution were resistant to NAL and/or CIP. Ten isolates with a point mutation at residue 86 were susceptible to NAL and CIP. This observation suggests that in addition to the substitution at residue 86 other mechanisms may confer resistance to quinolones. Further studies are needed to elucidate mechanisms for quinolone resistance in Campylobacter. The Campylobacter spp. isolated from chicken livers in this study were resistant to quinolones and other classes of antibiotics. [ABSTRACT FROM AUTHOR]

Published
2022
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42. Prevalence and Characterization of Quinolone Resistance in Campylobacterspp. Isolates in Chicken Livers from Retail Stores in Georgia, USA

Author

Yeh, Hung-Yueh, Cox, Nelson A., Hinton, Arthur, Berrang, Mark E., Plumblee Lawrence, Jodie R., and Thompson, Tori M.

Abstract

Campylobacteris a bacterial pathogen that causes human foodborne illnesses worldwide, and outbreaks have been associated with consumption of undercooked chicken livers. The objectives of this study were to compare two PCR assays of 250 Campylobacterisolates for identification to species, to assess antibiotic resistance of the isolates, and to analyze genetic diversity of the quinolone resistance determining regions (QRDRs) of the isolates. A double-blind design was used to identify the species of Campylobacter; 181 (72%) of the isolates were identified as Campylobacter jejuni,and 69 (28%) isolates were identified as Campylobacter coliby both PCR assays. A total of 93 (37.2%) isolates were resistant to at least one antibiotic. Among 88 C. jejuniisolates, 33 (18%) were resistant to nalidixic acid (NAL) and ciprofloxacin (CIP), 25 (14%) were resistant to tetracycline (TET), and 18 (10%) were resistant to NAL and TET. Two C. jejuniisolates were resistant to four of the tested antibiotics, and one isolate was resistant to five antibiotics. Two C. coliisolates were resistant to TET, and two were resistant to NAL, CIP, and TET. The amino acid sequences of the QRDRs for the isolates had eight point mutations and could be classified into 12 groups. Thirty-eight C. jejuniisolates resistant to NAL and CIP had a point mutation at residue 86 (substitution from threonine to isoleucine). However, six isolates without this substitution were resistant to NAL and/or CIP. Ten isolates with a point mutation at residue 86 were susceptible to NAL and CIP. This observation suggests that in addition to the substitution at residue 86 other mechanisms may confer resistance to quinolones. Further studies are needed to elucidate mechanisms for quinolone resistance in Campylobacter. The Campylobacterspp. isolated from chicken livers in this study were resistant to quinolones and other classes of antibiotics.

Published
2022
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43. Prevalence of ListeriaSpecies and Listeria monocytogeneson Raw Produce Arriving at Frozen Food Manufacturing Facilities

Author

Magdovitz, Brittany F., Gummalla, Sanjay, Garren, Donna, Thippareddi, Harshavardhan, Berrang, Mark E., and Harrison, Mark A.

Abstract

The ubiquity of Listeria monocytogenesin the environment affects the food industry and presents concerns for frozen food facilities. This study determined the prevalence and numbers of Listeriaspecies and L. monocytogeneson raw produce arriving at frozen food facilities. Raw produce was collected using multilevel blinding protocols to ensure anonymity of participants and avoid traceback. Five raw vegetables were selected: corn, carrots, green beans, peas, and spinach. Raw products were collected after arrival at the facilities but before cleaning or other preprocessing steps that are typically performed inside the facility. The U.S. Food and Drug Administration's Bacteriological Analytical Manualmethod for detection of Listeriaspp. and L. monocytogeneswas followed, with PCR screening followed by selective plating methods. Listerianumbers were estimated from positive samples using the most-probable-number (MPN) methodology. A total of 290 samples were collected, with 96 and 17 samples positive for Listeriaspp. (33.1%) and L. monocytogenes(5.9%), respectively. Enumeration data for the 96 Listeriaspp. samples indicated 82 samples had greater than 100 MPN of Listeriaspp. per g and 14 samples had less than 100 MPN Listeriaspp. per g. The prevalence of Listeriaspp. varied by commodity: spinach (66.7%), peas (50%), corn (32.2%), green beans (22.2%), and carrots (13%). L. monocytogenesprevalence was determined in corn (13.6%), peas (6.3%), and green beans (4.2%) arriving at processing facilities. Such data were previously unavailable to frozen vegetable processors and are valuable in implementing process control standards. The prevalence and pathogen concentration data from raw commodities found in this study can provide the industry with information to conduct more accurate quantitative risk assessments and a baseline to model and target appropriate pathogen reduction steps during processing.

Published
2021
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44. Recoverability of Listeria monocytogenes after coculture with Tetrahymena pyriformis.

Author

Meinersmann, Richard J., Berrang, Mark E., and Rigsby, Luanne L.

Subjects
*LISTERIA monocytogenes, *TETRAHYMENA pyriformis, *LISTERIA, *GENTAMICIN, *PROTOZOA
Abstract

Bactivory by protozoa is a major factor that limits the number of bacteria in nature and may control the presence of Listeria monocytogenes. The effectiveness of Tetrahymena pyriformis destruction of L. monocytogenes was measured. Within 1 hr, 35–40 T. pyriformis cells ingested an average of 1,219 CFU of L. monocytogenes. Gentamicin was then added to kill un‐ingested Listeria. In 24 hr, the recoverable bacteria were reduced at an exponential rate to undetectable levels (<1 per culture). A genetically diverse set of L. monocytogenes cultures all reduced Listeria recovery by the same degree. In assays without addition of gentamicin, numbers of attached L. monocytogenes cells were lessened from an average of log 6.5 CFU/2 ml culture to log 4.7 CFU/2 ml culture. T. pyriformis was capable of lowering numbers of both free‐swimming and attached L. monocytogenes. This technology may have applications to control L. monocytogenes in food processing environments. [ABSTRACT FROM AUTHOR]

Published
2020
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46. Enumeration of Escherichia coli cells on chicken carcasses as a potential measure of microbial process control in a random selection of slaughter establishments in the United States

Author

Altekruse, Sean F., Berrang, Mark E., Marks, Harry, Patel, Bharat, Shaw, William K., Jr., Saini, Parmesh, Bennett, Patricia A., and Bailey, J. Stan

Subjects
United States. Department of Agriculture -- Management, Escherichia coli -- Evaluation, Escherichia coli -- Environmental aspects, Food contamination -- Control, Company business management, Biological sciences
Abstract

A random sample from various large USDA-inspected operations is used for evaluating whether the number of Escherichia coli bacteria in carcass rinses from chicken slaughter establishments is monitored for the purpose of microbial process control. The relationships between Escherichia coli and Campylobacter and Salmonella are examined and the results regarding the reductions in Escherichia coli numbers have provided insight relevant to microbial process control.

Published
2009

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