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The m6A epitranscriptomic mark is the most abundant and widespread internal RNA chemical modification, which through the control of RNA acts as an important factor of eukaryote reproduction, growth, morphogenesis and stress response. The main m6A readers constitute a super family of proteins with hundreds of members that share a so-called YTH RNA binding domain. The majority of YTH proteins carry no obvious additional domain except for an Intrinsically Disordered Region (IDR). In Arabidopsis thaliana IDRs are important for the functional specialization among the different YTH proteins, known as Evolutionarily Conserved C-Terminal region, ECT 1 to 12. Here by studying the ECT2 protein and using an in vitro biochemical characterization, we show that full-length ECT2 and its YTH domain alone have a distinct ability to bind m6A, conversely to previously characterized YTH readers. We identify peptide regions outside of ECT2 YTH domain, in the N-terminal IDR, that regulate its binding to m6A-methylated RNA. Furthermore, we show that the selectivity of ECT2 binding for m6A is enhanced by a high uridine content within its neighbouring sequence, where ECT2 N-terminal IDR is believed to contact the target RNA in vivo. Finally, we also identify small structural elements, located next to ECT2 YTH domain and conserved in a large set of YTH proteins, that enhance its binding to m6A-methylated RNA. We propose from these findings that some of these regulatory regions are not limited to ECT2 or YTH readers of flowering plants but may be widespread among eukaryotic YTH readers.

Abstract Sudden infant death syndrome (SIDS) is the leading cause of post-neonatal infant mortality, but the underlying cause(s) are unclear. A subset of SIDS infants has abnormalities in the neurotransmitter, serotonin (5-hydroxytryptamine [5-HT]) and the adaptor molecule, 14–3–3 pathways in regions of the brain involved in gasping, response to hypoxia, and arousal. To evaluate our hypothesis that SIDS is, at least in part, a multi-organ dysregulation of 5-HT, we examined whether blood platelets, which have 5-HT and 14–3–3 signaling pathways similar to brain neurons, are abnormal in SIDS. We also studied platelet surface glycoprotein IX (GPIX), a cell adhesion receptor which is physically linked to 14–3–3. In infants dying of SIDS compared to infants dying of known causes, we found significantly higher intra-platelet 5-HT and 14–3–3 and lower platelet surface GPIX. Serum and plasma 5-HT were also elevated in SIDS compared to controls. The presence in SIDS of both platelet and brainstem 5-HT and 14–3–3 abnormalities suggests a global dysregulation of these pathways and the potential for platelets to be used as a model system to study 5-HT and 14–3–3 interactions in SIDS. Platelet and serum biomarkers may aid in the forensic determination of SIDS and have the potential to be predictive of SIDS risk in living infants.

Antecedentes: Los manglares son comunidades cuya estructura expresa patrones de acuerdo a las geoformas costeras en las que se desarrollan. Preguntas: ¿Existe una variación estructural de los manglares entre geoformas y regiones del Pacífico sur? ¿Cuál es el efecto del factor geomórfico-regional sobre la estructura de los manglares? ¿Cuál es el nivel de consistencia de la estructura de los manglares entre cada geoforma y región analizada? Descripción de datos: Área basal, altura, densidad, índice de complejidad y valor de importancia. Sitio de estudio: Manglares de 39 sistemas de cinco regiones hidrológicas en los estados de Guerrero, Oaxaca y Chiapas, México. Métodos: La clasificación estructural de los manglares se realizó considerando la geoforma (bocana, estero, estuario, laguna) y la región hidrológica. Se emplearon correlaciones para evaluar su relación con la salinidad y la recarga hídrica; y técnicas multivariadas para identificar diferencias entre los factores y patrones de agrupación. Resultados: La densidad y el área basal se correlacionaron positivamente con la recarga hídrica, y se encontró que la densidad y la complejidad del manglar variaron significativamente entre geoformas y regiones; mientras que el área basal y la altura fueron afectadas por geoforma y región. Los grupos discretos de las variables en las ordenaciones no resultó evidente, encontrándose mayor variabilidad estructural de los manglares por región hidrológica que por geoforma. Conclusiones: Existe variación estructural en los manglares entre geoformas y regiones del Pacífico sur, sin embargo, la variación del área basal, altura y densidad es baja, resultando consistente al compararse entre regiones hidrológicas.

Fluralaner is a recent external parasiticide, first of a new class of drugs (isoxazoline parasiticides). It is widely used in companion animals both for its wide spectrum (fleas, ticks and other mites) but also for its ease of use (oral tablets given once for 1 to three months). It is known to be eliminated primarily via the feces (>90%) as the unchanged compound. In zoo carnivores, controlling external parasites is also important and there are no specific products with a marketing authorization to control them. The first objective of this study was to evaluate the pharmacokinetic profile of fluralaner in zoo carnivores. The second objective was to demonstrate that fluralaner can be eliminated over a prolonged period of time, thereby raising questions about its potential impact on non-target species such as arthropods. After adjusting the oral dose using allometric equations, animals were dosed and fecal samples were collected on a regular basis for up to three months to determine the fecal elimination curve of fluralaner as a surrogate of plasma kinetics (for ethical and safety reasons, plasma samples were not considered). All samples were analyzed with a validated LC-MSMS technique. Our results show that, despite limitations and a limited number of animals included, most carnivores eliminate fluralaner in their feces for several weeks to months (in Lions, fluralaner was still detectable after 89 days). To our knowledge, this is the first study demonstrating such a long elimination period in animals. Further studies would be required to investigate the risk associated with the presence of active residues in other carnivore feces for the environment, especially in dogs and cats, considering the large use of this class of compounds.

Abstract Neurotensin-polyplex nanoparticles provide efficient gene transfection of nigral dopaminergic neurons when intracerebrally injected in preclinical trials of Parkinson’s disease because they do not cross the blood–brain barrier (BBB). Therefore, this study aimed to open BBB with focused ultrasound (FUS) on the substantia nigra to attain systemic and intranasal transfections and evaluate its detrimental effect in rats. Systemically injected Evans Blue showed that a two-pulse FUS opened the nigral BBB. Accordingly, 35 μL of neurotensin-polyplex nanoparticles encompassing the green fluorescent protein plasmid (79.6 nm mean size and + 1.3 mV Zeta-potential) caused its expression in tyrosine hydroxylase(+) cells (dopaminergic neurons) of both substantiae nigrae upon delivery via internal carotid artery, retro-orbital venous sinus, or nasal mucosa 30 min after FUS. The intracarotid delivery yielded the highest transgene expression, followed by intranasal and venous administration. However, FUS caused neuroinflammation displayed by infiltrated lymphocytes (positive to cluster of differentiation 45), activated microglia (positive to ionized calcium-binding adaptor molecule 1), neurotoxic A1 astrocytes (positive to glial fibrillary acidic protein and complement component 3), and neurotrophic A2 astrocytes (positive to glial fibrillary acidic protein and S100 calcium-binding protein A10), that ended 15 days after FUS. Dopaminergic neurons and axonal projections decreased but recuperated basal values on day 15 after transfection, correlating with a decrease and recovery of locomotor behavior. In conclusion, FUS caused transient neuroinflammation and reversible neuronal affection but allowed systemic and intranasal transfection of dopaminergic neurons in both substantiae nigrae. Therefore, FUS could advance neurotensin-polyplex nanotechnology to clinical trials for Parkinson’s disease.

Background and Aim: Probiotics can be used as an alternative to antibiotic growth promoters because antibiotics are prohibited worldwide. This study investigated the potential combination of probiotics and acidifiers to improve feed intake, productive performance, egg mass, and egg yolk chemical composition of late-laying quail for the health of humans who consume quail products. Materials and Methods: One hundred laying quails were divided into 4 × 5 treatments, with each group consisting of five replications. The adaptation period was 2 weeks, and the treatment was continued for 4 weeks. Probiotics and acidifiers were added to drinking water and incorporated into the diet. Feed and water were provided ad libitum. Treatment duration (1 week, 2 weeks, 3 weeks, and 4 weeks) and additional feed treatment (control, probiotic 2% + 0.5% acidifier, probiotic 2% + 1% acidifier, probiotic 4% + 0.5% acidifier, and probiotic 4% + 1% acidifier, respectively). Results: Significant differences (p < 0.05) were observed in feed intake, quail day production, feed efficiency, egg mass in laying quails, and the chemical composition of egg yolk with probiotics and acidifiers in late-laying quails. Conclusion: The combination of probiotics and acidifiers can improve feed intake, production performance, egg mass, and egg yolk chemical composition in late-laying quails.

The production of the common bean (Phaseolus vulgaris L.), one of the most important sources of protein and minerals and one of the most consumed grain legumes globally, is highly affected by heat and drought constraints. In contrast, the tepary bean (Phaseolus acutifolius A. Gray), a common bean-related species, is adapted to hot and dry climates. Hybridization to introduce complex traits from the tepary bean into the common bean has been challenging, as embryo rescue is required. In this study, we report three novel interspecific lines that were obtained by crossing lines from prior common bean × tepary bean hybridization with Phaseolus parvifolius Freytag in order to increase the male gametic diversity to facilitate interspecific crosses. These interspecific lines enhanced the crossability of the common bean and tepary bean species while avoiding the embryo rescue process. Crossing these three interspecific lines with tepary beans resulted in 12-fold more hybrid plants than crossing traditional common beans with tepary beans. Whole-genome sequencing analysis of these three interspecific lines shows large introgressions of genomic regions corresponding to P. parvifolius on chromosomes that presumably contribute to reproductive barriers between both species. The development of these lines opens up the possibility of increasing the introgression of desirable tepary bean traits into the common bean to address constraints driven by climate change.

New antimicrobial materials have drawn research and development attention due to antimicrobial resistance. Antimicrobial resistance is expected to pose a significant challenge to life in the future. This review comprehensively elucidates the potential of Myrmecodia spp. as an antibacterial agent by systematically selecting and reviewing the majority of relevant studies published in the past 10 years and retrieved from Scopus, PubMed, Web of Science, and related books. Myrmecodia spp. is a non-parasitic plant that grows as an epiphyte. These essential nutrients for the body, including flavonoids, alkaloids, polyphenols, tannins, and saponins, are found within plants. Myrmecodia spp.’s compound functions as an antibacterial agent. This review synthesizes information from multiple sources detailing Myrmecodia spp.’s antibacterial capacity through various testing methods.

Abstract In order to better understand the mechanisms generating genetic diversity in the recent allotetraploid species Coffea arabica, here we present a chromosome-level assembly obtained with long read technology. Two genomic compartments with different structural and functional properties are identified in the two homoeologous genomes. The resequencing data from a large set of accessions reveals low intraspecific diversity in the center of origin of the species. Across a limited number of genomic regions, diversity increases in some cultivated genotypes to levels similar to those observed within one of the progenitor species, Coffea canephora, presumably as a consequence of introgressions deriving from the so-called Timor hybrid. It also reveals that, in addition to few, early-occurring exchanges between homoeologous chromosomes, there are numerous recent chromosomal aberrations including aneuploidies, deletions, duplications and exchanges. These events are still polymorphic in the germplasm and could represent a fundamental source of genetic variation in such a lowly variable species.

[INLINE:1] Parkinsonism by unilateral, intranigral β-sitosterol β-D-glucoside administration in rats is distinguished in that the α-synuclein insult begins unilaterally but spreads bilaterally and increases in severity over time, thus replicating several clinical features of Parkinson's disease, a typical α-synucleinopathy. As Nurr1 represses α-synuclein, we evaluated whether unilateral transfected of rNurr1-V5 transgene via neurotensin-polyplex to the substantia nigra on day 30 after unilateral β-sitosterol β-D-glucoside lesion could affect bilateral neuropathology and sensorimotor deficits on day 30 post-transfection. This study found that rNurr1-V5 expression but not that of the green fluorescent protein (the negative control) reduced β-sitosterol β-D-glucoside-induced neuropathology. Accordingly, a bilateral increase in tyrosine hydroxylase-positive cells and arborization occurred in the substantia nigra and increased tyrosine hydroxylase-positive ramifications in the striatum. In addition, tyrosine hydroxylase-positive cells displayed less senescence marker β-galactosidase and more neuron-cytoskeleton marker βIII-tubulin and brain-derived neurotrophic factor. A significant decrease in activated microglia (positive to ionized calcium-binding adaptor molecule 1) and neurotoxic astrocytes (positive to glial fibrillary acidic protein and complement component 3) and increased neurotrophic astrocytes (positive to glial fibrillary acidic protein and S100 calcium-binding protein A10) also occurred in the substantia nigra. These effects followed the bilateral reduction in α-synuclein aggregates in the nigrostriatal system, improving sensorimotor behavior. Our results show that unilateral rNurr1-V5 transgene expression in nigral dopaminergic neurons mitigates bilateral neurodegeneration (senescence and loss of neuron-cytoskeleton and tyrosine hydroxylase-positive cells), neuroinflammation (activated microglia, neurotoxic astrocytes), α-synuclein aggregation, and sensorimotor deficits. Increased neurotrophic astrocytes and brain-derived neurotrophic factor can mediate the rNurr1-V5 effect, supporting its potential clinical use in the treatment of Parkinson's disease.

Estimating the emergency costs for disasters is of high relevance in the design of any comprehensive disaster risk management strategy. These costs usually include the ones associated with the immediate response aimed to provide the required safety and emergency attention, and those of debris removal and disposal. Over the time, and in different regions of the world, several research efforts have been carried out for the quantification of the emergency costs and have been usually associated with those of the direct losses. Also, previous studies have been typically carried out in the aftermath of large disasters, and to the best of our knowledge, no specific quantification of the emergency costs has been carried out in Central Asia. This paper presents a methodology, which has been applied in five countries in Central Asia using historical and synthetic events, to estimate the emergency costs as a function of modelled direct losses for earthquakes and floods, taking into account the demographic and building characteristics. The methodology allows the prospective estimation of the total emergency costs, so that they can be considered in the planning and budgeting of the emergency and recovery phases, as well as in disaster risk financing initiatives. It was found that the average emergency response costs for earthquakes and floods in Central Asia show good agreement with those previously estimated at other locations as a function of the direct losses; however, this methodology allows differentiating between different types of events allowing a better description and understanding of these needs.

Background: Mycoplasma synoviae is an important poultry pathogen causing heavy economic losses Worldwide. Subclinical persistence of this pathogen is the major issue to control its prevalence. Aim: This study aimed to determine the molecular and cross immunogenicity of Mycoplasma synoviae among broiler in five Districts of Khyber Pakhtunkhwa. Methods: This study was conducted by collecting 434 specimen samples from 40 broiler farms and desi poultry in five districts of Khyber Pakhtunkhwa. Specimen samples from the broiler birds (n=150), broiler farm environment (n= 264) and desi poultry birds (n=20) were aseptically collected and serially passaged in Modified Frey's broth. The homologous and heterologous antibody reactions were studied in rabbits. Prior to inoculation into rabbits the MS isolates were inactivated by formalin and adjuvanted with Montanide. Results: The overall turbidity prevalence in Frey's broth was observed as 109/434 (25.11%) samples and these turbidity positive samples were shifted on Frey's agar. After the appearance of classic fried egg colonies, the Biochemical confirmation was supported by production of Catalase and Phosphatase, reduction of Tetrazolium, Film and spot Assay and fermentation of Glucose for specie differentiation in avian mycoplasma. The MS prevalence percentage was recorded as 2% (9/434) through biochemical tests. The PCR results showed 0.5% MS prevalence with two field isolates (named as MS-1 and MS-2). Both MS-1 and MS-2 field isolates showed similar value (42.2) of homologous GMT titers. While the heterologous GMT for MS-1 serum against MS-2 isolate was lower (27.9) as compared to MS-2 serum against MS1 isolate (38.9). No titer was detected in control group (Group-III). Conclusion: In conclusion the results indicated the existence of MS in broiler birds and high homologous titers recorded between fields isolates which is a perpetual menace to poultry. [Open Vet J 2023; 13(10.000): 1299-1307]

Tepary bean (Phaseolus acutifolius A. Gray) is a drought and high ambient temperature tolerant crop native to the Sonoran Desert, the hottest and driest region in the United States and Mexico. Although tepary bean is an orphan crop with little current commercial production, there was a brief period of larger scale production in the early 1900s in California. Tepary bean has great potential as a novel crop in a warmer world climate and can be introduced as an alternative pulse crop in hot and/or dry regions worldwide. TARS-Tep 23 (Reg. no. GP-309, PI 698457) is an improved tepary bean germplasm with wide-ranging adaptation to tropical and temperate regions experiencing high temperature and drought stress conditions, with broad resistance to bean rust and with resistance to common bacterial blight. It has a flat, mottled black seed type with good seed size, a Type III plant habit, and a short crop cycle of 55–61 d in the environments tested. This germplasm was developed cooperatively by the USDA-ARS, Zamorano University, the University of California–Davis, and the University of Nebraska. The use of this improved germplasm by farmers in production zones affected by abiotic and biotic stresses, or by breeding programs, can potentially increase seed yields of this climate-resilient crop.

We present a meta-analysis of mangrove litterfall across 58 sites in the American Pacific, exploring its variability among geoforms, ecoregions, and provinces. This study contributes to filling the information gap on litter-based primary productivity in American mangroves at the ecoregional level and directly examines the effects of geomorphological and biogeographic factors on mangrove productivity. The objective was to evaluate how geoform, ecoregion, and province factors, along with eight environmental variables, influence litterfall-based primary productivity. Each site was categorized according to its landform through the analysis of satellite images obtained from various sensors on the Google Earth Pro v. 7.3.6 platform. Additionally, it was categorized according to its ecoregion and province by analyzing the occurrence of the sites on biogeographic unit coverage in ArcMap 10.4.1. We then analyzed the effect of each factor and the efficiency of categorization using multivariate methods. Our results showed significant differences in litterfall among the geoforms, with estuaries exhibiting higher litterfall production (11.90 Mg ha−1 year−1) compared to lagoons (7.49 ± 4.13 Mg ha−1 year−1). Differences were also observed among provinces, with the highest average in the Tropical Eastern Pacific (11.19 ± 3.63 Mg ha−1 year−1) and the lowest in the Warm Temperate Northeast Pacific (7.34 ± 4.28 Mg ha−1 year−1). Allocation success analyses indicated that sites classified by dominant species and province were more predictable (>60.34%) for litterfall production. Additionally, the maximum temperature and the precipitation of the wettest month and the driest month explained 34.13% of the variability in mangrove litter-based primary productivity. We conclude that mangrove litterfall production is influenced by coastal geomorphic characteristics and biogeography, which are, in turn, affected by latitude-induced climate variation.

The accurate forecasting of wind power has become a crucial task in renewable energy due to its inherent variability and uncertainty. This study addresses the challenge of predicting wind power generation without meteorological data by utilizing machine learning (ML) techniques on data from 2018 to 2021 from three wind farms in Guatemala. Various machine learning models, including Gated Recurrent Unit (GRU) and Long Short-Term Memory (LSTM), Bidirectional Long Short-Term Memory (BiLSTM), Bagging, and Extreme Gradient Boosting (XGBoost), were evaluated to determine their effectiveness. The performance of these models was assessed using Root Mean Squared Error (RMSE) and Mean Absolute Error (MAE) metrics. Time series cross-validation was employed to validate the models, with GRU, LSTM, and BiLSTM showing the lowest RMSE and MAE. Furthermore, the Diebold–Mariano (DM) test and Bayesian model comparison were used for pairwise comparisons, confirming the robustness and accuracy of the top-performing models. The results highlight the superior accuracy and robustness of advanced neural network architectures in capturing the complex temporal dependencies in wind power data, making them the most reliable models for precise forecasting. These findings provide critical insights for enhancing grid management and operational planning in the renewable energy sector.

Background: Metaldehyde poisoning in dogs is weel known and described issue. Several studies focused on the incidence, epidemiological features and clinical and pathological findings associated with this intoxication. However, there are no prospective studies of metaldehyde poisoning and late-onset seizures. Aims: To prospectively describe clinical signs, therapeutic management, outcomes, and delayed-onset seizures due to metaldehyde poisoning in dogs. Methods: A 15-month prospective study on dogs with a diagnosis of metaldehyde poisoning, either via phone call to the animal poison control centre or analysis at the toxicology laboratory in Lyon, France. Clinical signs, therapeutic management and outcomes and the late onset of seizures were assessed for at least 3 years. Results: Twenty-six dogs were enrolled in the study. The most prevalent clinical signs were ataxia (18 dogs), convulsions (17), hypersalivation (15), and tremors (15). Treatment was symptomatic (e.g., activated charcoal, emetic therapy, and IV fluids) with anticonvulsant therapy (mainly diazepam). The overall survival rate was 81% (21/26 dogs). All dogs that received active charcoal (11/11) or emetic therapy (4/4) survived. Twelve of 17 dogs had convulsions and survived; 9 were followed up for at least 3 years after poisoning, and none had any other seizure episode or neurological sequelae. Conclusion: This prospective study describes clinical signs, therapeutic management and outcome of metaldehyde poisoning in dogs and late onset neurologic sequelae. None of the 9 cases that were followed for 3 years developed neurological signs after metaldehyde poisoning. Therefore, long-term antiepileptic therapy is not indicated. [Open Vet J 2023; 13(5.000): 510-514]

[Introducción]: La vertiente Caribe de América Central se encuentra a barlovento de los vientos alisios asociados con la Alta Subtropical del Atlántico Norte y presenta un ciclo anual de precipitación bimodal, donde se observa un mínimo relativo en los meses de septiembre y octubre. [Objetivo]: Caracterizar el descenso observado en las lluvias en esta región en ese bimestre, estimando, para cada evento, las variables asociadas con los valores del día del inicio, día del mínimo, día del final, duración, intensidad y magnitud, en el periodo 1979-2010. [Metodología]: Se usaron 31 registros diarios de estaciones pluviométricas. Se examinó, en cada una de ellas, los meses de julio a noviembre, para encontrar los valores del mínimo, la fecha del mínimo, del inicio y del final, duración e intensidad. Se exploró la ocurrencia de alguna tendencia temporal o espacial en las características de los eventos. Por último, se revisaron diferentes índices climáticos y fuentes documentales para identificar las posibles causas sinópticas de los descensos más fuertes en las lluvias. [Resultados]: El inicio de los eventos se observó alrededor del 3 de septiembre, su mínimo el 23 de septiembre y el final 12 de octubre, con una duración aproximada de 39 días. Los eventos tienden a suceder primeramente en las estaciones ubicadas al noroeste del istmo. Los eventos más secos sucedieron bajo condiciones propicias para la ocurrencia de vientos con componente suroeste-oeste sobre la región. [Conclusiones]: El conocimiento sobre estas características de la precipitación ayuda en la planificación de sectores socioeconómicos claves en América Central ante eventuales eventos hidrometeorológicos adversos.

IntroductionAn adequate level of cardiorespiratory fitness (CRF) is critical for firefighters to perform the strenuous and physiologically demanding work of firefighting safely and effectively. The coronavirus disease 2019 (COVID-19) has been shown to negatively impact CRF in both the acute phase and longer-term following infection. This study aimed to determine changes to the CRF of firefighters pre- to post-mild to moderate COVID-19 infection and to investigate the impact of days past COVID-19 infection on change in CRF.MethodsCRF measures from cardiopulmonary exercise testing (CPET) at annual occupational health exams that occurred pre-COVID-19 infection in 2019 were obtained for firefighters from seven Arizona fire departments. Measures were compared to CPET evaluations from annual health exams the following year in a cohort of firefighters who self-reported mild to moderate illness following COVID-19 infection between exams.ResultsAmong a cohort of 103 firefighters, mean age 40 ± 9 years, CRF [as measured by peak oxygen consumption (VO2)] declined by an average of 2.55 ml·kg−1·min−1 or 7.3% (d = −0.38, p < 0.001) following COVID-19 infection (mean time from COVID-19 infection to CPET was 110 ± 78 days). The number of days past COVID-19 infection showed a small, yet significant, relationship to peak VO2 (r = 0.250, p = 0.011). Estimated marginal effects indicated that when biological sex, age, and BMI are controlled for, predicted peak VO2 returned to pre-COVID-19 values ~300 days after COVID-19 infection.ConclusionPeak VO2 (ml·kg−1·min−1) declined 7.3% among firefighters an average of 110 days past reporting mild to moderate COVID-19 infection. This decrease has implications for the operational readiness and safety of firefighters.

IntroductionProsocial behavior during childhood has been associated with numerous positive developmental and behavioral outcomes in adolescence and adulthood. Prosocial behavior, which includes cooperation and helping others, develops within a bioecological context. Considering it through such a lens enhances the understanding of the roles of different bioecological factors in its development.MethodsUsing data from a longitudinal study of adopted children and children reared with their biological parents, this paper examined if positive aspects of a child’s bioecological system at age 7 predict prosocial behavior in early adolescence (age 11), and whether these bioecological factors could offset risk due to biological family psychopathology and/or maternal prenatal substance use. The analyses incorporated variables from different levels of Bronfenbrenner’s bioecological model (the individual, microsystem, exosystem, and macrosystem) and examined the promotive, and potentially protective, effect of each contextual factor, while also considering their interplay with biological family psychopathology and prenatal substance use.ResultsResults from linear regression models indicated that the microsystem variable of parental warmth at age 7 had a promotive effect on age 11 prosocial behavior. Further, in addition to its main effect, parental warmth was protective against maternal substance use during pregnancy when children were raised with their biological parent (s). Household type (biological family) and biological family internalizing psychopathology were the only other significant predictors in the model, with each associated with lower prosocial behavior at age 11.DiscussionStudy results extend prior work on the benefits of parental warmth on child outcomes by employing a strength-based, bioecological approach to the development of prosocial behavior during early adolescence and examining “for whom” the effects of parental warmth are most protective.

Objetivos: O presente estudo objetiva investigar o valor prognóstico das razões hematológicas entre os componentes do hemograma em pacientes com câncer de mama em poliquimioterapia e relacioná-las às características clínico-patológicas da doença. Materiais e métodos: Trata-se de um estudo piloto. Pacientes encaminhadas para procedimento cirúrgico no Hospital do Câncer de Francisco Beltrão (CEONC) com lesões sugestivas de carcinoma ductal infiltrante mamário (CDI) foram convidadas a participar. Após assinatura do Termo de Consentimento Livre e Esclarecido houve a caracterização histopatológica da biópsia, pacientes com presença de lesões benignas foram excluídas do estudo. Foram incluídas 70 pacientes do sexo feminino portadoras de CDI. As coletas de sangue para realização do hemograma foram feitas ao diagnóstico (D0) e no início de todos os ciclos de tratamento (D21 até D168). Para análise dos dados houve avaliação temporal de D0 a D168, comparando todos os parâmetros, através da análise de variâncias ANOVA pareada para medidas repetidas. Foi considerado significativo p < 0,05. Todas as análises estatísticas foram conduzidas no software Graphpad Prism versão 9.0 (Graphpad software, San Diego, CA, USA). A pesquisa foi aprovada pelo Comitê de Ética Institucional sob CAAE nº35524814.4.0000.0107. Resultados: Dentre as razões analisadas, as maiores significâncias estatísticas foram observadas nas comparações das razões eritrócitos/leucócitos (ERI/LEU) e eritrócitos/linfócitos (ERI/LIN) em relação aos diferentes períodos de tempo (D0 vs. D84, D105 e D126) em cada grupo, com valor de p < 0,0001. Além disso, a relação ERI/LEU mostrou significância entre os períodos D0 vs. D42 e D0 vs. D63. A relação ERI/LIN também apresentou significância no período D0 vs. D63, ambas com valor de p < 0,05. A relação ERI/LIN quando relacionada com a presença de linfonodos positivos, mostrou-se significante no período D147 e quando relacionada com a presença de sítios metastáticos, foi significativa nos períodos D105 e D147. Além disso, a relação ERI/LEU, quando relacionada com a estratificação de risco, apresentou significância estatística nos períodos D0, D21 e D168. Paras estas razões apresentadas, os valores de p foram inferiores a 0,01. Discussão: A razão ERI/LIN mostrou significância no período D147 quando relacionada com a presença de linfonodos positivos, sugerindo aumento nos linfonodos afetados pela disseminação do câncer. Esta mesma razão apresentou significância em relação a presença de sítios metastáticos. No período D105, a razão ERI/LIN foi reduzida em pacientes com metástase e aumentada em D147, sugerindo que existem variações ao longo do tratamento que dependem do espelhamento da doença. Já a razão ERI/LEU apresentou-se significativamente reduzida em D0, D21 e D168 em pacientes estratificadas como alto risco de óbito e recidiva, ou seja, no início e final do primeiro esquema de tratamento quimioterápico, sugerindo que a relação ERI/LEU pode ser um indicador adicional na estratificação destas pacientes. Conclusão: Os resultados indicam que as razões hematológicas podem ser importantes indicadores de prognóstico para pacientes com câncer de mama submetidas a poliquimioterapia, especialmente em relação a desfechos de pior prognóstico, como presença de metástases a distância e estratificação de risco de óbito e recidiva.

A quimioterapia citotóxica permanece como a principal opção de tratamento nas leucemias linfoides agudas pediátricas (LLA), as quais correspondem ao tipo mais comum de câncer infantil na faixa-etária de 0 a 14 anos. Nesse estudo avaliou-se o perfil antioxidante através da medida da capacidade total antioxidante plasmática de amostras de sangue periférico de 34 pacientes diagnosticados com LLA do tipo B durante as fases de indução, consolidação e manutenção do tratamento quimioterápico com o protocolo do Grupo Brasileiro de Leucemias na Infância GBLTI- 2009. Amostras de sangue periférico anticoagulado foram coletadas ao diagnóstico (D0) e durante a indução (fase inicial), consolidação e manutenção. Na indução e consolidação, foram coletados e analisados os níveis de antioxidantes com um intervalo de 7 dias, sendo que na fase de indução foram agrupados em D0 (ao diagnóstico), D7, D14, D21 e D28, enquanto na fase de consolidação a estratificação foi realizada com grupos de 15 dias, no qual o D1 representa o início da consolidação e os demais foram agrupados em D7-14, D21-28, D35-42 e D46-D56. Por fim, ao final da quimioterapia, na fase de manutenção, no qual os agentes citotóxicos permanecem os mesmos, agrupou-se as amostras em D1 e após a cada 30 dias (D7-28 e D35-63), seguido por intervalo de 60 dias (D70-126, D133-182, D189-252) e ao final um grupo com intervalo de 120 dias (D252-370). As amostras foram centrifugadas a 4000 rpm por 5 minutos e o plasma obtido foi utilizado para avaliação da capacidade antioxidante total plasmática (TRAP) pela técnica de quimiluminescência de alta sensibilidade. Os níveis de antioxidantes foram elevados no D0 em relação ao D7 (28,68 - 1194,71 μM, p = 0,0178), sendo ainda maiores no grupo de alto risco (idade > 10 anos e/ou com contagem de células brancas > 50.000 células/m3 ao diagnóstico) em relação ao Baixo risco (253,79 – 1194,71 μM, p = 0,0314) e naqueles que evoluíram a óbito em comparação aos que sobreviveram (392,42 – 1194,71 μM, p = 0,0278). Além disso, durante a consolidação (56,14 – 352,05 μM, p =< 0,0001) e manutenção (30,48 – 672,99 μM, p =< 0,0001), houve redução significativa da capacidade antioxidante em relação a indução (28,68 - 1390,26 μM), alcançando níveis similares ao grupo controle (64,82 - 437,82 μM) no final do tratamento de manutenção. Esses achados sugerem que a variação da capacidade antioxidante total na LLA-B durante o tratamento quimioterápico é um parâmetro que possivelmente possa ser usado como preditor de prognostico.

Introdução e objetivos: Embora o mieloma múltiplo (MM) seja uma neoplasia com elevada taxa de letalidade, pouco se sabe das razões pelas quais alguns doentes sobrevivem muito mais tempo do que outros. Para avançar nesse entendimento, uma investigação foi conduzida no perfil transcriptômico das células-tronco derivadas de pacientes com MM, comparando-se os resultados clínicos de morte e sobrevida seis meses após o transplante de medula óssea, para avaliar como as vias de sinalização imunológicas e os fatores inflamatórios influenciam na patogênese do MM. Materiais e métodos: Os produtos de leucaférese de 39 pacientes com MM elegíveis para transplante autólogo foram recolhidos e analisados. Após a extração, o RNA foi analisado pelo método GeneChip Human Exon 1.0 Array. O perfil do transcriptoma foi analisado in silico, e as vias de sinalização diferencialmente expressas foram validadas. Resultados e discussão: Os resultados mostraram diferenças na expressão de genes relacionados à inflamação, vias de resposta imunológica e estresse oxidativo. A análise in silico também apontou o envolvimento do fator de transcrição NFκB na modulação desses genes. Assim, foram selecionadas, para validação, moléculas participantes nesses processos, incluindo a dosagem de citocinas TNF-α, IFN-γ e TGF-β1, além dos mediadores da sinalização de estresse oxidativo (perfil pró-oxidante, capacidade antioxidante da amostra e cálculo do índice de estresse). Os níveis de TNF-α foram significativamente reduzidos em doentes que morreram e que tinham mais de 50 anos de idade no momento do diagnóstico, e em doentes com plasmacitoma. O aumento de TNF-α foi detectado em doentes com níveis muito elevados de β2-microglobulina. A redução de IFN-γ foi observada em doentes com uma resposta completa ao tratamento, em comparação com os doentes com uma resposta muito boa. Os doentes com plasmacitoma também tinham um perfil pró-oxidante aumentado. Conclusão: Estes dados mostram o perfil de marcadores de resposta inflamatória que são alterados em pacientes com MM que morrem rapidamente, e servem como base para o desenvolvimento de futuros estudos de marcadores de melhor sobrevivência nessa doença.

Summary: Here, we present a detailed protocol to study the role of a human nuclear m6A RNA reader, YTHDC1, on chromatin-associated RNA targets. We describe steps for RNA extraction coupled to subnuclear fractionation to identify and study RNA-based regulations that take place in the chromatin-associated fraction. We then detail an RNA immunoprecipitation procedure adapted to identify chromatin-associated RNA targets. This protocol can be adapted to other human or mammalian chromatin-associated RNA binding proteins.For complete details on the use and execution of this protocol, please refer to Timcheva et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Plant domestication has strongly modified crop morphology and development. Nevertheless, many crops continue to display atavistic characteristics that were advantageous to their wild ancestors but are deleterious under cultivation, such as pod dehiscence (PD). Here, we provide the first comprehensive assessment of the inheritance of PD in the common bean (Phaseolus vulgaris), a major domesticated grain legume. Using three methods to evaluate the PD phenotype, we identified multiple, unlinked genetic regions controlling PD in a biparental population and two diversity panels. Subsequently, we assessed patterns of orthology among these loci and those controlling the trait in other species. Our results show that different genes were selected in each domestication and ecogeographic race. A chromosome Pv03 dirigent-like gene, involved in lignin biosynthesis, showed a base-pair substitution that is associated with decreased PD. This haplotype may underlie the expansion of Mesoamerican domesticates into northern Mexico, where arid conditions promote PD. The rise in frequency of the decreased-PD haplotype may be a consequence of the markedly different fitness landscape imposed by domestication. Environmental dependency and genetic redundancy can explain the maintenance of atavistic traits under domestication.

Genetic analyses and utilization of wild genetic variation for crop improvement in common bean (Phaseolus vulgaris L.) have been hampered by yield evaluation difficulties, identification of advantageous variation, and linkage drag. The lack of adaptation to cultivation conditions and the existence of highly structured populations make association mapping of diversity panels not optimal. Joint linkage mapping of nested populations avoids the later constraint, while populations crossed with a common domesticated parent allow the evaluation of wild variation within a more adapted background. Three domesticated by wild backcrossed-inbred-line populations (BC1S4) were developed using three wild accessions representing the full range of rainfall of the Mesoamerican wild bean distribution crossed to the elite drought tolerant domesticated parent SEA 5. These populations were evaluated under field conditions in three environments, two fully irrigated trials in two seasons and a simulated terminal drought in the second season. The goal was to test if these populations responded differently to drought stress and contained progenies with higher yield than SEA 5, not only under drought but also under water-watered conditions. Results revealed that the two populations derived from wild parents of the lower rainfall regions produced lines with higher yield compared to the domesticated parent in the three environments, i.e., both in the drought-stressed environment and in the well-watered treatments. Several progeny lines produced yields, which on average over the three environments were 20% higher than the SEA 5 yield. Twenty QTLs for yield were identified in 13 unique regions on eight of the 11 chromosomes of common bean. Five of these regions showed at least one wild allele that increased yield over the domesticated parent. The variation explained by these QTLs ranged from 0.6 to 5.4% of the total variation and the additive effects ranged from -164 to 277 kg ha-1, with evidence suggesting allelic series for some QTLs. Our results underscore the potential of wild variation, especially from drought-stressed regions, for bean crop improvement as well the identification of regions for efficient marker-assisted introgression.

Zinc phosphide has been recently approved in Europe as a vole control product. Currently, only one formulation (lentils/pellets) is marketed with 0.8% Zn3P2. It is applied with a delivery device for burrow baiting. In many instances, zinc phosphide poisoning has been confirmed in non-target species (primary poisoning). In order to be prepared for potential non-target poisoning incidents in wildlife, the SAGIR network, FREDON Franche-Comté, and University of Franche-Comté conducted a field study on common voles to test the sampling method and storage impact under realistic field conditions on the detection of zinc phosphide. The toxicology laboratory of Vetagro Sup, member of SAGIR, worked on the improvement of the World Health Organization WHO technique in order to lower the Limit of Quantification (LOQ) and to validate the technique for the correct identification of field cases. The specificity was tested on 20 gastric content samples (100%), and the LOQ was established at 0.01 g/l (i.e., a 100-fold decrease as compared with the 1995 WHO technique). Zinc content was measured by Flame Atomic Absorption Spectroscopy and non-poisoned animals were tested to check baseline values and to estimate recovery of spiked samples (94-102%). Quantification of zinc in the liver of poisoned versus control animals was also performed. A total of 30 voles were collected in treated and control fields and submitted for analysis. Technicians were not aware of the poisoning status of the animals when performing analytical investigations. Twenty-one individuals were trapped in the control area and nine in the treated fields. Phosphine and high levels of Zn could be detected in eight of the nine intoxicated individuals. Phosphine was not detected in the control voles. Zinc concentrations in the gastric content were significantly different between negative control and exposed animals, but liver concentrations of zinc were similar.

Genetic analyses and utilization of wild genetic variation for crop improvement in common bean (Phaseolus vulgaris L.) have been hampered by yield evaluation difficulties, identification of advantageous variation, and linkage drag. The lack of adaptation to cultivation conditions and the existence of highly structured populations make association mapping of diversity panels not optimal. Joint linkage mapping of nested populations avoids the later constraint, while populations crossed with a common domesticated parent allow the evaluation of wild variation within a more adapted background. Three domesticated by wild backcrossed-inbred-line populations (BC1S4) were developed using three wild accessions representing the full range of rainfall of the Mesoamerican wild bean distribution crossed to the elite drought tolerant domesticated parent SEA 5. These populations were evaluated under field conditions in three environments, two fully irrigated trials in two seasons and a simulated terminal drought in the second season. The goal was to test if these populations responded differently to drought stress and contained progenies with higher yield than SEA 5, not only under drought but also under water-watered conditions. Results revealed that the two populations derived from wild parents of the lower rainfall regions produced lines with higher yield compared to the domesticated parent in the three environments, i.e., both in the drought-stressed environment and in the well-watered treatments. Several progeny lines produced yields, which on average over the three environments were 20% higher than the SEA 5 yield. Twenty QTLs for yield were identified in 13 unique regions on eight of the 11 chromosomes of common bean. Five of these regions showed at least one wild allele that increased yield over the domesticated parent. The variation explained by these QTLs ranged from 0.6 to 5.4% of the total variation and the additive effects ranged from -164 to 277 kg ha-1, with evidence suggesting allelic series for some QTLs. Our results underscore the potential of wild variation, especially from drought-stressed regions, for bean crop improvement as well the identification of regions for efficient marker-assisted introgression.

Abstract Background Ophionyssus natricis is the main species of mite that infests captive reptiles. High infestations may result in the host experiencing general discomfort and deleterious effects, even death. Moreover, O. natricis is an important vector of reptile vector-borne diseases and is considered to be the putative vector of the Reptarenavirus, the causal agent of the inclusion body disease. Despite the cosmopolitan distribution of O. natricis in captive reptiles, treatment options are limited. The aim of the present study was to assess the efficacy of afoxolaner (NexGard®; Boehringer Ingelheim, Ingelheim, Germany) in heavily infested, privately owned snakes, evaluate the prevalence of mites and drug availability in the plasma of treated snakes (pharmacokinetics) and perform a clinical examination of animals. Methods The study was conducted in two snake breeding facilities, where many snakes were infested with mites. Each animal was clinically examined and weighed, and mite infestations were assessed on the animals and in their enclosures (environment). Animals were treated with a dose of 2.5 mg afoxolaner per kilogram body weight (2.5 mg/kg) administered orally. All animals were examined pre-treatment (T0) and at various time points post-treatment (T1, 6 h; T2, 24 h; T3, 14 days; T4, 28 days). The collected mites were morphologically identified at the species level and the species identity also confirmed molecularly. Results Overall, 81 snakes from the two participating facilities (i.e. 70 from site 1 and 11 from site 2) were screened, and 31 (38.3%) snakes were found to have at least one mite. All mites were identified morphologically and molecularly as O. natricis. Lampropeltis was the genus of snakes with highest number of infested individuals. Mites were found to be alive on snakes at T1, but at T2 only dead mites were observed, and at T3 and T4 mites were no longer present on the animals or in their environment. No side effects were observed in the treated snakes. Conclusions A single oral administration of afoxolaner at 2.5 mg/kg was a safe treatment for snakes and 100% effective for the eradication of natural O. natricis infestation without the need to treat the environment of the snake. Graphical Abstract

BackgroundCommon bean is the most important staple grain legume for direct human consumption and nutrition. It complements major sources of carbohydrates, including cereals, root crop, or plantain, as a source of dietary proteins. It is also a significant source of vitamins and minerals like iron and zinc. To fully play its nutritional role, however, its robustness against stresses needs to be strengthened. Foremost among these is drought, which commonly affects its productivity and seed quality. Previous studies have shown that photosynthate remobilization and partitioning is one of the main mechanisms of drought tolerance and overall productivity in common bean.ResultsIn this study, we sought to determine the inheritance of pod harvest index (PHI), a measure of the partitioning of pod biomass to seed biomass, relative to that of grain yield. We evaluated a recombinant inbred population of the cross of ICA Bunsi and SXB405, both from the Mesoamerican gene pool, to determine the effects of intermittent and terminal drought stresses on the genetic architecture of photosynthate allocation and remobilization in pods of common bean. The population was grown for two seasons, under well-watered conditions and terminal and intermittent drought stress in one year, and well-watered conditions and terminal drought stress in the second year. There was a significant effect of the water regime and year on all the traits, at both the phenotypic and QTL levels. We found nine QTLs for pod harvest index, including a major (17% of variation explained), stable QTL on linkage group Pv07. We also found eight QTLs for yield, three of which clustered with PHI QTLs, underscoring the importance of photosynthate remobilization in productivity. We also found evidence for substantial epistasis, explaining a considerable part of the variation for yield and PHI.ConclusionOur results highlight the genetic relationship between PHI and yield and confirm the role of PHI in selection of both additive and epistatic effects controlling drought tolerance. These results are a key component to strengthen the robustness of common bean against drought stresses.

BackgroundWild crop relatives have been potentially subjected to stresses on an evolutionary time scale prior to domestication. Among these stresses, drought is one of the main factors limiting crop productivity and its impact is likely to increase under current scenarios of global climate change. We sought to determine to what extent wild common bean (Phaseolus vulgaris) exhibited adaptation to drought stress, whether this potential adaptation is dependent on the climatic conditions of the location of origin of individual populations, and to what extent domesticated common bean reflects potential drought adaptation.MethodsAn extensive and diverse set of wild beans from across Mesoamerica, along with a set of reference Mesoamerican domesticated cultivars, were evaluated for root and shoot traits related to drought adaptation. A water deficit experiment was conducted by growing each genotype in a long transparent tube in greenhouse conditions so that root growth, in addition to shoot growth, could be monitored.ResultsPhenotypic and landscape genomic analyses, based on single-nucleotide polymorphisms, suggested that beans originating from central and north-west Mexico and Oaxaca, in the driest parts of their distribution, produced more biomass and were deeper-rooted. Nevertheless, deeper rooting was correlated with less root biomass production relative to total biomass. Compared with wild types, domesticated types showed a stronger reduction and delay in growth and development in response to drought stress. Specific genomic regions were associated with root depth, biomass productivity and drought response, some of which showed signals of selection and were previously related to productivity and drought tolerance.ConclusionsThe drought tolerance of wild beans consists in its stronger ability, compared with domesticated types, to continue growth in spite of water-limited conditions. This study is the first to relate bean response to drought to environment of origin for a diverse selection of wild beans. It provides information that needs to be corroborated in crosses between wild and domesticated beans to make it applicable to breeding programmes.

BackgroundWild crop relatives have been potentially subjected to stresses on an evolutionary time scale prior to domestication. Among these stresses, drought is one of the main factors limiting crop productivity and its impact is likely to increase under current scenarios of global climate change. We sought to determine to what extent wild common bean (Phaseolus vulgaris) exhibited adaptation to drought stress, whether this potential adaptation is dependent on the climatic conditions of the location of origin of individual populations, and to what extent domesticated common bean reflects potential drought adaptation.MethodsAn extensive and diverse set of wild beans from across Mesoamerica, along with a set of reference Mesoamerican domesticated cultivars, were evaluated for root and shoot traits related to drought adaptation. A water deficit experiment was conducted by growing each genotype in a long transparent tube in greenhouse conditions so that root growth, in addition to shoot growth, could be monitored.ResultsPhenotypic and landscape genomic analyses, based on single-nucleotide polymorphisms, suggested that beans originating from central and north-west Mexico and Oaxaca, in the driest parts of their distribution, produced more biomass and were deeper-rooted. Nevertheless, deeper rooting was correlated with less root biomass production relative to total biomass. Compared with wild types, domesticated types showed a stronger reduction and delay in growth and development in response to drought stress. Specific genomic regions were associated with root depth, biomass productivity and drought response, some of which showed signals of selection and were previously related to productivity and drought tolerance.ConclusionsThe drought tolerance of wild beans consists in its stronger ability, compared with domesticated types, to continue growth in spite of water-limited conditions. This study is the first to relate bean response to drought to environment of origin for a diverse selection of wild beans. It provides information that needs to be corroborated in crosses between wild and domesticated beans to make it applicable to breeding programmes.

The purpose of this research was to determine the effect of combination probiotics and Moringa oleifera (M. oleifera) extract in nutrient consumption of ducks. This study used 48 Peking ducks. The concentration of probiotic was 1.2 x 108 CFU/ml. The treatments of this research were P0 (control), P1 (4 ml probiotics), P2 (4 ml M. oleifera extract) and P3 (2 ml probiotics + 2 ml M. oleifera extract). The data were statistically analyzed by Analysis of Variance (ANOVA). The results showed that feed consumption of dry matter, organic matter, crude protein, crude fat, crude fiber and nitrogen free extract were no significant difference (p>0.05) between all treatments. It can be concluded that the addition of combination probiotics and M. oleifera extract can be used to maintain nutrient consumption and safe for health ducks.

Abstract Groundwater monitoring is the highest tier in the leaching assessment of plant protection products in the EU. The European Commission requested EFSA for a review by the PPR Panel of the scientific paper of Gimsing et al. (2019) on the design and conduct of groundwater monitoring studies. The Panel concludes that this paper provides many recommendations; however, specific guidance on how to design, conduct and evaluate groundwater monitoring studies for regulatory purposes is missing. The Panel notes that there is no agreed specific protection goal (SPG) at EU level. Also, the SPG has not yet been operationalised in an agreed exposure assessment goal (ExAG). The ExAG describes which groundwater needs to be protected, where and when. Because the design and interpretation of monitoring studies depends on the ExAG, development of harmonised guidance is not yet possible. The development of an agreed ExAG must therefore be given priority. A central question in the design and interpretation of groundwater monitoring studies is that of groundwater vulnerability. Applicants must demonstrate that the selected monitoring sites represent realistic worst‐case conditions as specified in the ExAG. Guidance and models are needed to support this step. A prerequisite for the regulatory use of monitoring data is the availability of complete data on the use history of the products containing the respective active substances. Applicants must further demonstrate that monitoring wells are hydrologically connected to the fields where the active substance has been applied. Modelling in combination with (pseudo)tracer experiments would be the preferred option. The Panel concludes that well‐conducted monitoring studies provide more realistic exposure assessments and can therefore overrule results from lower tier studies. Groundwater monitoring studies involve a high workload for both regulators and applicants. Standardised procedures and monitoring networks could help to reduce this workload.

Currently, a smart city should ideally be environmentally friendly and sustainable, and energy management is one method to monitor sustainable use. This research project investigates the potential for a “smart city” to improve energy management by enabling the adoption of various types of intelligent technology to improve the energy sustainability of a city’s infrastructure and operational efficiency. In addition, the South Korean smart city region of Songdo serves as the inspiration for this case study. In the first module of the proposed framework, we place a strong emphasis on the data capabilities necessary to generate energy statistics for each of the numerous structures. In the second phase of the procedure, we employ the collected data to conduct a data analysis of the energy behavior within the microcities, from which we derive characteristics. In the third module, we construct baseline regressors to assess the proposed model’s varying degrees of efficacy. Finally, we present a method for building an energy prediction model using a deep learning regression model to solve the problem of 48-hour-ahead energy consumption forecasting. The recommended model is preferable to other models in terms of R2, MAE, and RMSE, according to the study’s findings.

We analyzed the nucleotide variability and the expression profile of DREB genes from common bean, a crop of high economic and nutritional value throughout the world but constantly affected by abiotic stresses in cultivation areas. As DREB genes have been constantly associated with abiotic stress tolerance, we systematically categorized 54 putative PvDREB genes distributed in the common bean genome. It involved from AP2 domain location and amino acid conservation analysis (valine at the 14th position) to the identification of conserved motifs within peptide sequences representing six subgroups (A-1 to A-6) of PvDREB proteins. Four genes (PvDREB1F, PvDREB2A, PvDREB5A, and PvDREB6B) were cloned and analyzed for their expression profiles under abiotic stresses and their nucleotide and amino acid diversity in genotypes of Andean and Mesoamerican origin, showing distinct patterns of expression and nucleotide variability. PvDREB1F and PvDREB5A showed high relative inducibilities when genotypes of common bean were submitted to stresses by drought, salt, cold, and ABA. PvDREB2A inducibility was predominantly localized to the stem under drought. PvDREB6B was previously described as an A-2 (DREB2) gene, but a detailed phylogenetic analysis and its expression profile clearly indicated it belongs to group A-6. PvDREB6B was found as a cold- and dehydration-responsive gene, mainly in leaves. Interestingly, PvDREB6B also showed a high nucleotide and amino acid diversity within its coding region, in comparison to the others, implicating in several nonsynonymous amino acid substitutions between Andean and Mesoamerican genotypes. The expression patterns and nucleotide diversity of each DREB found in this study revealed fundamental characteristics for further research aimed at understanding the molecular mechanisms associated with drought, salt, and cold tolerance in common bean, which could be performed based on association mapping and functional analyses.