67 results on '"Bernabé DG"'
Search Results
2. Head and neck squamous cell carcinoma in young patients: a 26-year clinicopathologic retrospective study in a Brazilian specialized center
- Author
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Tomo, S., primary, Neto, SC., additional, Collado, FU., additional, Sundefeld, ML., additional, Bernabé, DG., additional, Biasoli, ÉR., additional, and Miyahara, GI., additional
- Published
- 2020
- Full Text
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3. Salivary alpha amylase and cortisol levels in children with global developmental delay and their relation with the expectation of dental care and behavior during the intervention.
- Author
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Santos MJ, Bernabé DG, Nakamune AC, Perri SH, de Aguiar SM, and de Oliveira SH
- Published
- 2012
4. A rare development of classical Hodgkin lymphoma in the head and neck region: Case report and review of the literature.
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Pereira MAS, Santos GR, Legarrea JMA, Kayahara GM, Fonseca FP, Xavier-Junior JCC, Miyahara GI, Bernabé DG, Urazaki MS, Cortopassi GM, and Valente VB
- Subjects
- Humans, Male, Middle Aged, Tonsillar Neoplasms pathology, Tonsillar Neoplasms diagnosis, Palatine Tonsil pathology, Head and Neck Neoplasms pathology, Head and Neck Neoplasms diagnosis, Hodgkin Disease pathology, Hodgkin Disease diagnosis
- Abstract
Background: Classical Hodgkin lymphoma (CHL) is characterized by a proliferation of malignant cells of the lymphoreticular system and often involves lymph nodes, spleen, liver, and bone marrow; it is rare in the head and neck region., Case Description: A 58-year-old man had an enlargement with ulceration in the left palatine tonsil that was causing dysphagia. Microscopic examination revealed an infiltrate of large, atypical lymphoid cells positive for cluster of differentiation 30, cluster of differentiation 15, PAX5, and Epstein-Barr virus. Complementary tests initially ruled out other sites of the disease. The results led to diagnosis of a rare development of CHL in the palatine tonsil, which was staged as IIEB. Before therapy was initiated, nodal lesions developed in the neck and the CHL was restaged as IIB. The patient was treated successfully with a regimen of doxorubicin, bleomycin, vinblastine, and dacarbazine. After a review of the literature, the authors found only 3 cases with the clinical, imaging, and microscopic features of primary CHL of the palatine tonsil., Practical Implications: Despite being a rare event, CHL may first develop in extranodal sites, such as the palatine tonsil. In this context, the role of the dentist is pivotal for early diagnosis of the disease. Investigations into the development of primary tonsillar CHL in the oropharynx are needed because the disease has a different clinical course than nodal lesions., Competing Interests: Disclosure None of the authors reported any disclosures., (Copyright © 2024 American Dental Association. Published by Elsevier Inc. All rights reserved.)
- Published
- 2024
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5. The emerging meanings of spirituality in oral medicine practice.
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Santos-Silva AR and Bernabé DG
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- Humans, Oral Medicine, Spirituality
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- 2024
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6. Expression of β 1 - and β 2 -adrenergic receptors in oral squamous cell carcinoma and their association with psychological and clinical factors.
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Santos-Sousa AL, Kayahara GM, Bastos DB, Sarafim-Silva BAM, Crivelini MM, Valente VB, Corrente JE, Xavier-Júnior JCC, Miyahara GI, and Bernabé DG
- Subjects
- Humans, Receptors, Adrenergic, beta-2 metabolism, Squamous Cell Carcinoma of Head and Neck, Receptors, Adrenergic, beta, Fatigue, Pain, Carcinoma, Squamous Cell pathology, Mouth Neoplasms pathology, Head and Neck Neoplasms
- Abstract
Background: Psychological stressors have been related to tumor progression through the activation of beta-adrenergic receptors (β-AR) in several types of cancer., Purpose: This study aimed to investigate the expressions of β
1 - and β2 -AR and their association with psychological and clinicopathological variables in patients with oral squamous cell carcinoma., Methods: Tumor samples from 99 patients diagnosed with OSCC were subjected to immunohistochemical reaction to detect the expression of β1 -AR and β2 -AR. Anxiety and depression symptoms were assessed using the Beck Anxiety Inventory and Beck Depression Inventory (BDI), respectively. The Brunel Mood Scale was used for measuring affective mood states., Results: Univariate analyzes revealed that higher expression of β1 -AR was associated with increased alcohol consumption (p = 0.032), higher education (p = 0.042), worse sleep quality (p = 0.044) and increased levels of pain related to the primary tumor (p < 0.001). Higher expression of β2 -AR was related with regional metastasis (p = 0.014), increased levels of pain related to the primary tumor (p = 0.044), anxiety (p < 0.001) and depressive (p = 0.010) symptoms and higher mood scores of angry (p = 0.010) and fatigue (p = 0.010). Multivariate analysis identified that patients with advanced clinical stage had lower β1 -AR expression (OR=0.145, 95% CI=0.025-0.828, p = 0.003). Higher anxiety symptoms and higher mood fatigue are independent factors for increased β2 -AR expression (OR=4256, 95% CI=1439-12606, p = 0.009; OR=3816, 95% CI=1258-11,573, p = 0.018, respectively)., Conclusion: This study reveal that anxiety, fatigue symptoms, and clinical staging are associated with tumor expression of beta-adrenergic receptors in patients with oral cancer., Competing Interests: Declaration of Competing Interest The authors declare that they have no conflict of interest., (Copyright © 2024. Published by Elsevier Ltd.)- Published
- 2024
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7. Ulcerated lesion in the hard palate of a child.
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Cardoso DM, Daltoé MO, da Silva LAB, Miyahara GI, Rocha AC, Bernabé DG, and Valente VB
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- Humans, Diagnosis, Differential, Male, Child, Female, Palate, Hard pathology, Oral Ulcer pathology, Oral Ulcer etiology
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- 2024
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8. Primary manifestation of HIV-related Burkitt lymphoma in the oral and maxillofacial regions.
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Silva Filho NJD, Cardoso DM, Kayahara GM, Xavier-Júnior JCC, Cortopassi GM, Fonseca FP, Miyahara GI, Bernabé DG, and Valente VB
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- Female, Humans, Adult, Herpesvirus 4, Human, Burkitt Lymphoma complications, Burkitt Lymphoma diagnosis, Burkitt Lymphoma pathology, Epstein-Barr Virus Infections diagnosis, Epstein-Barr Virus Infections pathology, HIV Infections complications, HIV Infections diagnosis
- Abstract
Background: Burkitt lymphoma (BL) is a subtype of non-Hodgkin lymphoma. It is strongly associated with HIV infection and has a highly aggressive clinical course. The involvement of the maxillofacial region in BL has rarely been reported., Case Description: A 36-year-old woman with HIV-positive status had painless bilateral swelling of the oral mucosa and middle and lower thirds of the face. Microscopic analysis of the oral lesion revealed an atypical lymphoid infiltrate with a starry sky pattern. The lymphoid cells expressed cluster of differentiation 20, cluster of differentiation 10, B-cell lymphoma 6, and c-Myc; the Ki-67 proliferative index was high. The tumor cells were positive for Epstein-Barr virus. These results led to the diagnosis of HIV-related BL., Practical Implications: BL and other immunodeficiency-related lymphoproliferative malignancies may affect the oral and maxillofacial regions and should be included in the differential diagnosis of rapidly expanding swelling in young patients., Competing Interests: Disclosure None of the authors reported any disclosures., (Copyright © 2024 American Dental Association. Published by Elsevier Inc. All rights reserved.)
- Published
- 2024
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9. Bilateral intra-lymph node Warthin's tumor as a potential diagnostic pitfall mimicking metastatic carcinoma.
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Santos-Sousa AL, Camilo-Júnior DJ, Conrado-Neto S, Bernabé DG, and Xavier-Júnior JCC
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- Humans, Parotid Gland pathology, Lymph Nodes pathology, Parotid Neoplasms diagnosis, Parotid Neoplasms pathology, Adenolymphoma diagnosis, Adenolymphoma pathology, Carcinoma pathology
- Abstract
Warthin's tumor (WT) is a benign and frequent salivary gland tumor primarily affecting the parotid gland. In some cases, this tumor can involve the extra parotid region and affect cervical lymph nodes. Fine-needle aspiration can be the first step in the diagnostic approach to lymphadenopathy; however, specimens from intra-nodal WT can present a potential pitfall, leading to a misdiagnosis of metastasis. Here, we report an unusual case of a patient with bilateral WT in parotid lymph nodes misdiagnosed as metastases. In addition, we highlight the cytopathological aspects of WT to alert cytopathologists about this challenging diagnosis., (© 2023 Wiley Periodicals LLC.)
- Published
- 2024
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10. A pilot study to improve pain phenotyping in head and neck cancer patients.
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Ye Y, Cardoso DM, Kayahara GM, and Bernabé DG
- Abstract
Pain associated with head and neck cancer (HNC) is difficult to manage and reduces quality of life. It has been increasingly recognized that HNC patients exhibit a wide range of pain symptoms. Here we developed an orofacial pain assessment questionnaire and conducted a pilot study to improve pain phenotyping in HNC patients at the diagnosis. The questionnaire captures the following pain characteristics: pain intensity, location, quality, duration, and frequency; the impact of pain on daily activities; changes in smell and food sensitivities. Twenty-five HNC patients completed the questionnaire. 88% patients reported pain at the site of tumor; 36% reported multiple pain sites. All patients with pain reported at least one neuropathic pain (NP) descriptor, 54.5% reported at least two NP descriptors. The most common descriptors were "burning" and "pins and needles". Most patients reported increased pain to sour or hot/spicy food/drinks, and to food with coarse/hard textures. Patients exhibited impaired oral function, especially chewing, talking, mouth/jaw opening, and eating. Tumor progression has a significant impact on pain. Nodal metastasis is linked to pain at multiple body sites. Patients with advanced tumor staging experience greater pain at the primary tumor site, when exposed to hot or spicy food/drinks or food with hard/coarse texture, or when eating or chewing. We conclude that HNC patients experience a wide range of pain symptoms with altered mechanical, chemical, and temperature sensation. Improved phenotyping and stratification of pain in HNC patients will help address the underlying etiology, which may enable personalized therapeutic approaches in the future., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The reviewer T.Y. is currently organizing a Research Topic with the author Y.Y., (© 2023 Ye, Cardoso, Kayahara and Bernabé.)
- Published
- 2023
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11. Influence of different methods for classification of lymph node metastases on the survival of patients with oral squamous cell carcinoma.
- Author
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Tomo S, de Castro TF, Araújo WAF, Collado FU, Neto SC, Biasoli ÉR, Bernabé DG, and Miyahara GI
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- Humans, Lymph Nodes surgery, Lymph Nodes pathology, Squamous Cell Carcinoma of Head and Neck, Neoplasm Staging, Lymphatic Metastasis diagnosis, Lymphatic Metastasis pathology, Retrospective Studies, Carcinoma, Squamous Cell diagnosis, Carcinoma, Squamous Cell surgery, Mouth Neoplasms diagnosis, Mouth Neoplasms surgery, Head and Neck Neoplasms
- Abstract
Background: Despite the advances in the classification of oral squamous cell carcinoma (OSCC) based on its extension by the TNM system, there is still a need for methods to better classify the patients to predict prognosis and indicate adjuvant therapy., Objectives: To analyze the influence of the number of positive lymph nodes (PN), lymph node ratio (LNR), and log odds of positive lymph nodes (LODDS) in survival of patients with OSCC., Methods: Clinicopathologic data from patients with OSCC who were treated with curative purposes by surgery and neck dissection (ND) with or without subsequent adjuvant therapies from 1991 to 2015 was retrospectively assessed. The impact of the PN, LNR, LODDS, and other variables on overall survival (OS) and disease-free survival (DFS) was analyzed in univariate and multivariate analyses., Results: One hundred nineteen patients were included in this study. In the univariate analysis the PN had a significant impact on OS (p = 0.001) and DFS (p = 0.020), and the LNR had a significant impact on the OS (p = 0.042). In the multivariate analysis with other relevant clinicopathologic variables, the PN was the only significantly independent factor influencing in the OS (p = 0.017) but not in DFS (p = 0.096)., Conclusions: The PN is an independent prognostic indicator for OS and DFS in patients with OSCC and has the potential to aggregate the current AJCC classification. The LNR has potential to be an important prognostic indicator, but the methods for this classification require lapidation. The LODDS did not demonstrate prognostic potential., Competing Interests: Declaration of Competing Interest The authors declare that there is no potential conflict of interest regarding this work., (Copyright © 2022. Published by Elsevier Masson SAS.)
- Published
- 2023
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12. Early life stress exacerbates bone resorption and inhibits anxiety-like behaviour induced by apical periodontitis in rats.
- Author
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Bertolini Botelho MC, Cintra LTA, da Silva CC, Mitsuy Kayahara G, Belzunces Pereira R, Oliveira Santos MF, Issamu Miyahara G, Biasoli ÉR, Penha Oliveira SH, and Bernabé DG
- Subjects
- Animals, Male, Rats, Anxiety, Maternal Deprivation, Rats, Wistar, Stress, Psychological, Alveolar Bone Loss, Bone Resorption, Periapical Periodontitis pathology
- Abstract
Aim: To evaluate the influence of the early life stress (ELS) on the severity of the apical periodontitis (AP) in Wistar rats., Methodology: Forty male Wistar rats were divided into four groups (n = 10): Control rats; AP-rats with AP; ELS-rats subject to ELS; AP + ELS-rats exposed to ELS and subject to AP. ELS was induced by maternal separation (MS) for a period of 3 h for 21 consecutive days. AP was induced via pulp exposure of the first and second right maxillary molars to the oral environment for 40 days. Three days before euthanasia, all rats underwent behavioural analysis to measure anxiety levels by elevated zero maze. Then, the rats were euthanized and the maxillas were removed to assess the occurrence and severity of AP. The periapical region was evaluated for the intensity of the inflammatory infiltrate and the extent of bone loss. The Mann-Whitney test was performed for nonparametric data, and the Tukey's or Student's t-test was performed for parametric data (p < .05)., Results: The intensity of the inflammatory infiltrate was significantly larger in the AP + ELS group when compared with AP group (p < .05). The AP + ELS group exhibited significantly greater alveolar bone loss, with a periapical lesion size of 103.5 ± 29.88, compared with 72.3 ± 22.28 in the AP group (p < .05). Rats with AP displayed higher anxiety-like behaviour in relation to the control group (p < .05). However, exposure to ELS abolished the AP-induced increased anxiety-like 'behaviour' throughout, since that rats from AP + ELS group attended more the open arms than non-stressed rats with AP (p < .05)., Conclusion: Early life stress is predictive of the severity of AP exacerbating the inflammatory process and increasing periapical bone resorption., (© 2022 British Endodontic Society. Published by John Wiley & Sons Ltd.)
- Published
- 2023
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13. Reply to the opinion paper "The impact of stress on rat tongue carcinogenesis induced by 4-nitroquinoline 1-oxide: some theoretical concepts for scientific debate" by Silva et al.
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Kayahara GM, Verza FA, Valente VB, Miyahara GI, and Bernabé DG
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- Rats, Animals, Carcinogens toxicity, Tongue, Oxides, 4-Nitroquinoline-1-oxide toxicity, Carcinogenesis chemically induced
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- 2023
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14. Mantle cell lymphoma involving the oral and maxillofacial region: a study of 20 cases.
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Carvalho MVR, Rodrigues-Fernandes CI, de Cáceres CVBL, Mesquita RA, Martins MD, Román Tager EMJ, Carlos R, Pires FR, Robinson L, van Heerden WFP, Valente VB, Xavier-Júnior JCC, Bernabé DG, Pontes HAR, Soares CD, da Costa MHM, Vargas PA, and Fonseca FP
- Subjects
- Adult, Humans, Male, Aged, Female, Cyclin D1, Herpesvirus 4, Human, Lymphoma, Mantle-Cell diagnosis, Lymphoma, Mantle-Cell pathology, Epstein-Barr Virus Infections
- Abstract
Objective: To investigate the clinicopathologic features of mantle cell lymphoma (MCL) involving the oral and maxillofacial region., Methods: The MCL cases were retrieved from the pathosis database of 6 pathology laboratories. Original hematoxylin and eosin slides and immunohistochemical reactions were reviewed for confirmation of the initial diagnosis. Clinical data of the cases were obtained from the patients' pathosis and/or medical charts., Results: Twenty cases were included in the study, showing a male predominance and a mean age of 66 years. The oral cavity (12 cases) and the oropharynx (5 cases) were the most commonly involved subsites. Most cases presented as asymptomatic swellings, with 2 cases showing bilateral involvement of the palate. The classic histologic variant predominated (12/20 cases). All cases expressed CD20 with nuclear cyclin D1 positivity. SOX11 was seen in 9/13 cases, CD5 in 6/16 cases, Bcl2 in 16/19 cases, CD10 in 2/20 cases, and Bcl6 in 4/16 cases. Ki67 showed a mean proliferation index of 40.6%. The Epstein-Barr virus (EBV) was negative in all cases investigated. Follow-up data was available for 7 patients, with 5 currently alive and 2 deceased., Conclusion: Mantle cell lymphoma, albeit rare, may manifest in the oral and maxillofacial region. Its histologic heterogeneity demands a high degree of diagnostic skill from pathologists., (Copyright © 2022 Elsevier Inc. All rights reserved.)
- Published
- 2023
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15. Potential of lymph-node ratio as a prognostic factor for patients with oral squamous cell carcinoma.
- Author
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Tomo S, Araújo WAF, de Castro TF, Neto SC, Collado FU, Biasoli ÉR, Bernabé DG, and Miyahara GI
- Subjects
- Humans, Lymph Node Ratio, Squamous Cell Carcinoma of Head and Neck, Prognosis, Retrospective Studies, Lymphatic Metastasis diagnosis, Lymphatic Metastasis pathology, Lymph Nodes surgery, Lymph Nodes pathology, Neoplasm Staging, Mouth Neoplasms diagnosis, Mouth Neoplasms surgery, Carcinoma, Squamous Cell diagnosis, Carcinoma, Squamous Cell surgery, Head and Neck Neoplasms
- Abstract
Background: To analyze the influence of the lymph node ratio (LNR) in survival of patients with OSCC METHODS: Clinicopathologic data from patients with OSCC who were treated with curative surgery and neck dissection (ND) with or without adjuvant therapies from 1991 to 2015 was retrospectively assessed. The impact of LNR and other variables on overall survival (OS) and disease-free survival (DFS) was analyzed in univariate and multivariate analyses., Results: One hundred nineteen patients were included. In the univariate analysis the LNR had a significant impact on OS (p = 0.01) and DFS (p = 0.01). In the multivariate analysis, the LNR was the only significantly independent factor influencing in the OS (p = 0.03). The adjuvant therapies did not influence on the OS (p = 0.42) and DFS (p = 0.10)., Conclusions: The LNR is an independent prognostic factor in patients with OSCC. The LNR alone is not recommended to indicate the performance of adjuvant therapies., Competing Interests: Declaration of Competing Interest The authors declare that there is no potential conflict of interest regarding this work., (Copyright © 2022 Elsevier Masson SAS. All rights reserved.)
- Published
- 2022
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16. Pleomorphic sarcoma of maxillary sinus: Clinical report of a patient initially diagnosed with denture-induced fibrous hyperplasia.
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Valente VB, Kayahara GM, Bastos DB, Santos IDS, Xavier-Junior JCC, Biasoli ÉR, Miyahara GI, and Bernabé DG
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- Humans, Female, Aged, Maxillary Sinus diagnostic imaging, Hyperplasia pathology, Dentures, Sarcoma diagnostic imaging, Sarcoma pathology, Soft Tissue Neoplasms pathology, Histiocytoma, Malignant Fibrous diagnosis, Histiocytoma, Malignant Fibrous pathology
- Abstract
Undifferentiated pleomorphic sarcoma is a high-grade soft-tissue malignant tumor that is rare in the head and neck region. A 74-year-old woman displayed a large nodular lesion in the maxillary alveolar mucosa, which was initially identified as denture-related fibrous hyperplasia. Although her prosthodontist has adjusted the maxillary complete denture to relieve pressure on the lesion, it increased in size over time. Computed tomography images of the maxillary sinus showed an extensive destructive lesion. A biopsy was performed, and microscopic examination revealed a poorly differentiated malignancy with numerous spindle cells. Immunohistochemistry reactions were negative for CD45, CD30, CD68, CD34, cytokeratin, S100, desmin, and smooth muscle actin. These findings led to the diagnosis of an undifferentiated pleomorphic sarcoma of the maxillary sinus., (Copyright © 2021 Editorial Council for the Journal of Prosthetic Dentistry. Published by Elsevier Inc. All rights reserved.)
- Published
- 2022
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17. Assessment of the knowledge of canalis sinuosus amongst dentists and dental students: An online-based cross-sectional study.
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Lopes-Santos G, Salzedas LMP, Bernabé DG, Ikuta CRS, Miyahara GI, and Tjioe KC
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- Cone-Beam Computed Tomography methods, Cross-Sectional Studies, Dentists, Education, Dental, Humans, Maxilla blood supply, Students, Dental
- Abstract
Introduction: Canalis sinuosus (CS) is a neurovascular canal that corresponds to a small branch of the infraorbital canal. This study aimed at assessing the knowledge and detection performance of CS amongst dentists and dental students., Materials and Methods: Four-hundred and five dentists and dental students answered a questionnaire with three parts: 1. Socio-demographical; 2. Clinical cases with cone- beam computed tomography (CBCT) sections showing CS and 3. Previous knowledge about CS. The chi-squared test and Spearman's correlation test were used to compare results as appropriate. p-values below .05 were considered statistically significant., Results: Most participants did not identify CS in any CBCT. There was an association between the number of correct answers and dental specialties. Most individuals had not learned about CS previously. There was an association between past knowledge of CS and gender, highest academic degree, working environment, dental specialty and number of correct answers but not with age or experience in Dentistry., Conclusion: This study suggests that most dentists are not aware about CS and do not know how to diagnose it. Previous knowledge about CS positively influenced its identification in CBCT., (© 2021 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
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- 2022
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18. A submucous yellow nodule of the fornix.
- Author
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Miyahara GI, de Castro TF, Araújo WAF, Tomo S, Biasoli ER, Santos-Silva AR, Xavier-Júnior JCC, Crivelini MM, and Bernabé DG
- Subjects
- Female, Humans, Vagina
- Published
- 2022
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19. Propranolol inhibits cell viability and expression of the pro-tumorigenic proteins Akt, NF-ĸB, and VEGF in oral squamous cell carcinoma.
- Author
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Shibuya CM, Tjioe KC, Oliveira SHP, and Bernabé DG
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- Cell Line, Tumor, Cell Movement drug effects, Cell Proliferation drug effects, Cell Survival drug effects, Humans, Mouth Neoplasms drug therapy, Mouth Neoplasms metabolism, Mouth Neoplasms pathology, NF-kappa B antagonists & inhibitors, NF-kappa B biosynthesis, Propranolol pharmacology, Proto-Oncogene Proteins c-akt antagonists & inhibitors, Proto-Oncogene Proteins c-akt biosynthesis, Squamous Cell Carcinoma of Head and Neck drug therapy, Squamous Cell Carcinoma of Head and Neck metabolism, Squamous Cell Carcinoma of Head and Neck pathology, Vascular Endothelial Growth Factor A antagonists & inhibitors, Vascular Endothelial Growth Factor A biosynthesis
- Abstract
Background: Propranolol (PPL) has been suggested as an option for the treatment of various types of cancer. However, data regarding its effectiveness against oral cancer are scarce. Thus, we aimed to evaluate the antitumor potential of PPL in oral squamous cell carcinoma (OSCC) in vitro., Methods: OSCC cell lines, SCC-9, SCC-25, and Cal27, were treated with PPL at different times and concentrations. OSCC cells were treated with PPL alone or in combination with cisplatin (CDDP) or 5-fluorouracil (5-FU). Cell viability was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The expression of phosphorylated (p)-Akt, p-S6, p-PTEN, p-P65, and VEGF was verified by immunofluorescence. The migratory activity of OSCC cells was evaluated using a wound-healing assay., Results: PPL reduced OSCC cell viability in a dose- and time-dependent manner. Concentrations above 300 µM, 110 µM, and 100 µM for SCC-9, Cal27, and SCC-25, respectively, significantly eliminated tumor cells. The combination of PPL with CDDP and 5-FU enhanced their antitumor effects. There was a modest difference between the use of the IC30 and IC50 of PPL in the combinatory options. PPL downregulated p-P65 NF-ĸB and VEGF expression in SCC-9 and Cal27 cells but not in SCC-25 cells. PPL inhibited the phosphorylation of Akt and s6 and increased the phosphorylation of PTEN in all OSCC cell lines studied. PPL inhibited OSCC cell migration after 24 h of treatment., Conclusion: PPL was effective against oral cancer cells and enhanced standard-of-care. PPL inhibited cell viability and the expression of pAkt, NF-ĸB, and VEGF., (Copyright © 2022 Elsevier Ltd. All rights reserved.)
- Published
- 2022
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20. Stress hormone norepinephrine incites resistance of oral cancer cells to chemotherapy.
- Author
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Tjioe KC, Cardoso DM, Oliveira SHP, and Bernabé DG
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- ATP Binding Cassette Transporter, Subfamily G, Member 2 metabolism, ATP-Binding Cassette Transporters metabolism, ATP-Binding Cassette Transporters pharmacology, Cell Line, Tumor, Cisplatin pharmacology, Drug Resistance, Multiple, Drug Resistance, Neoplasm, Hormones pharmacology, Humans, Neoplasm Proteins metabolism, Norepinephrine pharmacology, Antineoplastic Agents pharmacology, Mouth Neoplasms drug therapy
- Abstract
This study investigated whether norepinephrine (NE) and epinephrine (E) interfere in the response of head and neck squamous cell carcinoma (SCC) cell lines to cisplatin and explored the mechanisms of chemoresistance. Head and neck SCC-derived cell lines SCC-9, Cal27, SCC-25, and FaDu were stimulated with NE or E and treated with the inhibitory concentration of cisplatin for 24 h. As for adrenergic receptors (ADRB) inhibition, cells were treated with propranolol. The results showed that, when combined with NE, cisplatin effectiveness against SCC-9 and Cal27 but not SCC-25 and FaDu cells were notably reduced. E did not affect the response of the cells to cisplatin. Further experiments were performed with the responsive SCC-9 and SCC-25 cell lines and the hormone NE. The time course assay showed that stimulation of oral SCC cells with NE decreased the cleavage of caspase-3 and expression of multidrug resistance protein 1 (MDR-1) but only transiently affected ATP-binding cassette (ABC) subfamily G, isoform 2 protein (ABCG2) expression. The expression of cleaved caspase-3 and Bcl-2 were, respectively, decreased and increased by the combination of NE and cisplatin in SCC-9 and Cal27 cells. NE-induced resistance was reverted by previous treatment with propranolol. Expressions of ABCG2, and p-Akt but not of MDR-1, were enhanced by NE plus cisplatin when compared to cisplatin only in both cell lines. Migratory activity of oral SCC cells challenged with cisplatin was not affected by NE. These findings reveal for the first time that stress hormone NE induces resistance of oral cancer cells to cisplatin in vitro through the ADRB/Akt/ABCG2 pathway, pumping the drug out of the cell and inhibiting apoptosis.
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- 2022
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21. HIV-related plasmablastic lymphoma causing extensive bone destruction in the mandible.
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Kayahara GM, Valente VB, Salzedas LMP, Passador-Santos F, Furuse C, Biasoli ÉR, Miyahara GI, and Bernabé DG
- Subjects
- Adult, Female, Humans, Mandible pathology, Mouth pathology, HIV Infections complications, Lymphoma, Large B-Cell, Diffuse complications, Plasmablastic Lymphoma complications, Plasmablastic Lymphoma diagnosis, Plasmablastic Lymphoma pathology
- Abstract
Plasmablastic lymphoma (PBL) is a rare subtype of large B-cell lymphoma commonly associated with HIV infection. HIV-related PBL has a dismal prognosis. The aggressive clinical course of the disease may lead to the development of rapid-growing swellings, like several benign and malignant conditions. Herein, we reported the case of a 38-year-old woman with a painful swelling in the mandible initially diagnosed as an abscess derived from a tooth extraction. Intraoral examination revealed a painful swelling with reddish, white and purplish areas in the posterior region of the mandible without signs of infection. Imaging exams showed an extensive bone destruction in the left mandibular body. Histopathological examination revealed a high proliferation of plasmacytoid cells with nuclear hyperchromatism. Tumor cells were negative for CD20, and positive for Ki-67, CD138, IgG and lambda chain. The diagnosis of oral PBL was defined and serological test showed positivity for HIV. Eight months after starting treatment, the patient died due to complications of cancer treatment. Lymphoproliferative malignancies related to HIV infection should be included in the differential diagnosis of rapid-growing swellings in the oral cavity., (Copyright © 2022 Elsevier Ltd. All rights reserved.)
- Published
- 2022
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22. Interventions for Radiation-Induced Fibrosis in Patients With Breast Cancer: Systematic Review and Meta-analyses.
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Nogueira RMP, Vital FMR, Bernabé DG, and Carvalho MB
- Abstract
Purpose: Radiation therapy can affect normal tissues in patients with breast cancer, causing adverse effects such as fibrosis. Although there are several interventions for radiation-induced fibrosis, the efficacy of these procedures is still unclear. The purpose of this review is to evaluate the efficacy of interventions for radiation-induced fibrosis in patients with breast cancer., Methods and Materials: This is a systematic review of randomized clinical trials. Studies that compared any intervention for fibrosis to another intervention, placebo, or no intervention were included. Outcomes assessed were fibrosis, adverse events, quality of life, treatment adherence, pain, and functionality., Results: A total of 2501 publications were found, and 7 studies were selected because they met the inclusion criteria. The interventions for fibrosis were pentoxifylline and vitamin E, grape seed extract, kinesiotherapy, and endermotherapy. The results showed great heterogeneity in the treatment protocols for radiation-induced fibrosis in patients with breast cancer and in their evaluation metrics. The meta-analyses showed no benefit in using pentoxifylline and vitamin E compared with placebo or no intervention (standardized mean difference: -0.30; 95% confidence interval, -0.79 to 0.20; P = .24 [very low evidence]) compared with placebo and vitamin E (standardized mean difference: -0.09; 95% confidence interval, -0.66 to 0.49; P = .77 [moderate evidence]), respectively, assessed by the Late Effects Normal Tissue Task Force-Subjective, Objective, Management, and Analytic (LENT-SOMA) scoring scale., Conclusions: The effectiveness of these interventions for the treatment of radiation-induced fibrosis in patients with breast cancer could not be determined. Although isolated studies show significant results favorable to the experimental groups, caution should be exercised in these findings because of the small number, small sample size, and high risk of bias presented by some of the included studies, which makes the recommendation for clinical practice still weak., (© 2022 The Authors.)
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- 2022
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23. Predisposing factors for increased cortisol levels in oral cancer patients.
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Figueira JA, Sarafim-Silva BAM, Gonçalves GM, Aranha LN, Lopes FL, Corrente JE, Biasoli ÉR, Miyahara GI, and Bernabé DG
- Abstract
Cancer patients may have a dysregulation of the hypothalamic-pituitary-adrenal (HPA) axis and abnormal secretion of cortisol. Increased cortisol levels have been associated with worse prognosis in patients with different types of tumors. Although anxiety and depression can trigger an abnormal cortisol secretion, little is known regarding the influence of these emotional disorders on HPA axis dysregulation in cancer patients when evaluating together with demographic, clinicopathological and biobehavioral variables. This cross-sectional study analyzed the pre-treatment plasma cortisol levels of 133 patients with oral squamous cell carcinoma (OSCC) and its association with demographic, clinicopathological, biobehavioral and psychological variables. Plasma cortisol levels were measured by electrochemiluminescence, and anxiety and depression symptoms were assessed using Beck Anxiety Inventory (BAI) and Depression (BDI), respectively. Demographic, clinicopathological and biobehavioral data were collected from patients' medical records. Results from multivariate analysis showed that the occurrence of cancer-induced pain was predictive for higher cortisol levels (OR = 5.388, p = 0.003). Men with OSCC were 4.5 times more likely to have higher plasma cortisol levels than women (OR = 4.472, p = 0.018). The effect of sex on cortisol concentrations was lost in the adjusted model for clinical staging (OR = 2.945, p = 0.116). The absence of chronic alcohol consumption history was a protective factor for highest hormone concentrations in oral cancer patients (OR = 0.104, p = 0.004). Anxiety symptoms measured by BAI as "hands trembling" (OR = 0.192, p = 0.016) and being "nervous" (OR = 0.207, p = 0.0004) were associated with lower cortisol levels. In contrast, the feeling of "fear of losing control" was a risk factor for highest hormone concentrations (OR = 6.508, p = 0.0004). The global score and specific symptoms of depression measured by the BDI were not predictive for plasma hormone levels (p > 0.05). Together, our results show that pain, alcohol consumption and feeling fear are independent factors for increased systemic cortisol levels in patients with oral cancer. Therefore, psychological intervention, as well as control of pain and alcohol consumption, should be considered to prevent the negative effects of cortisol secretion dysregulation in cancer patients., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2022 The Authors.)
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- 2022
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24. The 'AEIOU' system to identify primary oral melanoma.
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Xavier-Junior JCC, Ocanha-Xavier JP, Asato MA, and Bernabé DG
- Subjects
- Humans, Sensitivity and Specificity, Melanoma diagnosis, Melanoma pathology, Mouth Neoplasms diagnosis, Skin Neoplasms pathology
- Abstract
Primary oral melanoma (POM) is a rare entity that is often asymptomatic and is associated with a poor prognosis. Following the example of the ABCDE acronym for the clinical diagnosis of early cutaneous melanoma, we would like to introduce another acronym, AEIOU, to identify lesions that are clinically suspicious for POM. The letter "A" means age older than 50; "E" means ethnicity in reference to the higher occurrence among Asians, Hispanics, and Africans; "I" means irregularity in reference to irregular borders or color; "O" means oral palate, the most frequent site of POM; and "U" means ulceration. To the best of our knowledge, this paper is the first to describe an acronym AEIOU as a diagnostic aid for POM among health practitioners and the general population. Future studies should test the acronym's sensitivity and specificity for POM diagnosis in clinical practice., (Copyright © 2021 Elsevier Ltd. All rights reserved.)
- Published
- 2022
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25. Blastoid variant of mantle cell lymphoma in palatine tonsil.
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Valente VB, de Castro TF, Takamiya AS, Callestini R, Xavier-Junior JCC, Cortopassi GM, Neto SC, Collado FU, Biasoli ÉR, Miyahara GI, and Bernabé DG
- Subjects
- Aged, Humans, Lymph Nodes, Male, Lymphoma, Mantle-Cell diagnosis, Lymphoma, Mantle-Cell drug therapy, Palatine Tonsil pathology
- Abstract
Blastoid variant of mantle cell lymphoma (MCL) is an aggressive and extremely rare malignancy. MCL may be diagnosed in lymph nodes and/or extranodal sites exhibiting a poor prognosis. MCL with primary presentation in palatine tonsils has been rarely reported. Herein, we report the case of a 73-year-old man with a painless nodular mass on the right palatine tonsil. A biopsy was performed, and microscopic analysis revealed a neoplasm composed of small to medium sized lymphocytes with finely dispersed chromatin, roundish nucleus and many mitoses. The tumor cells were positive for CD20 (L26), CD5 (4C7), Cyclin D1 (EP12), Bcl2 (124) and Ki-67 (MIB-1; 90%), and negative for Bcl6 (PG-B6p), MUM1 (MUM1p) and CD3 (Polyclonal). These findings led to the diagnosis of blastoid variant of MCL. Diagnostic workup with computed tomography scan excluded other sites of disease. The patient was treated successfully with cyclophosphamide, doxorubicin, vincristine and prednisolone (mini-CHOP regimen). Although the blastoid variant of MCL is rare, it should be included in the differential diagnosis of rapid-growing masses in the palatine tonsil., (Copyright © 2021. Published by Elsevier Ltd.)
- Published
- 2021
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26. Catecholamines Mediate Psychologic Stress-Induced Cancer Progression.
- Author
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Bernabé DG
- Subjects
- Dopamine, Epinephrine, Norepinephrine, Stress, Psychological, Catecholamines, Neoplasms
- Abstract
Catecholamines, which are involved in response to physical or emotional stress, have emerged as one of the main mediators of the relationship between chronic stress and cancer progression. The study in this issue of Cancer Research by Liu and colleagues reveals a new mechanism by which psychologic stress stimulates cancer progression through the D2 dopamine receptor and activation of the oxygen-independent HIF1α pathway. Although most investigations so far have focused on the action of the stress-related catecholamines norepinephrine and epinephrine on tumor cells, this study shows that dopamine and its receptor can be a potential therapeutic target. The findings broaden the understanding of the interaction of catecholamines with the tumor microenvironment and reinforces the need to look at psychologic stress as a modulator of cancer progression. See related article by Liu et al., p. 5353 ., (©2021 American Association for Cancer Research.)
- Published
- 2021
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27. Stress hormones promote DNA damage in human oral keratinocytes.
- Author
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Valente VB, de Melo Cardoso D, Kayahara GM, Nunes GB, Tjioe KC, Biasoli ÉR, Miyahara GI, Oliveira SHP, Mingoti GZ, and Bernabé DG
- Subjects
- 8-Hydroxy-2'-Deoxyguanosine metabolism, Apoptosis, DNA Breaks drug effects, Epithelial Cells, Histones metabolism, Hormones pharmacology, Humans, Hydrocortisone pharmacology, Keratinocytes drug effects, Nitrosamines chemistry, Nitrosamines pharmacology, Norepinephrine pharmacology, Oxidation-Reduction, Nicotiana chemistry, DNA Damage drug effects, Hormones metabolism, Keratinocytes metabolism, Mouth Mucosa metabolism, Stress, Physiological
- Abstract
Chronic stress increases the systemic levels of stress hormones norepinephrine and cortisol. As well as tobacco-specific carcinogen NNK (4-(methylnitrosamine)-1-(3-pyridyl)-1-butanone), they can induce expressive DNA damage contributing to the cancer development. However, it is unknown whether stress hormones have genotoxic effects in oral keratinocytes. This study investigated the effects of stress hormones on DNA damage in a human oral keratinocyte cell line (NOK-SI). NOK-SI cells stimulated with norepinephrine or cortisol showed higher DNA damage compared to untreated cells. Norepinephrine-induced DNA damage was reversed by pre-treatment with beta-adrenergic blocker propranolol. Cells treated with NNK combined to norepinephrine displayed reduced levels of caspases 3 and 7. Cortisol also reduced the activity of pro-apoptotic enzymes. NNK or norepinephrine promoted single-strand breaks and alkali-label side breaks in the DNA of NOK-SI cells. Pre-treatment of cells with propranolol abolished these effects. Carcinogen NNK in the presence or absence of cortisol also induced DNA damage of these cells. The genotoxic effects of cortisol alone and hormone combined with NNK were blocked partially and totally, respectively, by the glucocorticoid receptor antagonist RU486. DNA damage promoted by NNK or cortisol and carcinogen combined to the hormone led to intracellular γH2AX accumulation. The effects caused by NNK and cortisol were reversed by propranolol and glucocorticoid receptor antagonist RU486, respectively. Propranolol inhibited the oxidation of basis induced by NNK in the presence of DNA-formamidopyrimidine glycosylase. DNA breaks induced by norepinephrine in the presence or absence of NNK resulted in higher 8OHdG cellular levels. This effect was also induced through beta-adrenergic receptors. Together, these findings indicate that stress hormones induce DNA damage of oral keratinocytes and could contribute to oral carcinogenesis., (© 2021. The Author(s).)
- Published
- 2021
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28. Tongue Nodule as Primary Manifestation of American Cutaneous Leishmaniasis in an Immunocompetent Patient.
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Botelho MCB, Ferreira LL, Fikaris S, Prado-Ribeiro AC, Soubhia AMP, Biasoli ER, Miyahara GI, and Bernabé DG
- Subjects
- Antiprotozoal Agents therapeutic use, Humans, Leishmaniasis, Mucocutaneous drug therapy, Male, Meglumine Antimoniate therapeutic use, Middle Aged, Tongue Diseases pathology, Leishmaniasis, Mucocutaneous diagnosis, Leishmaniasis, Mucocutaneous pathology, Tongue Diseases diagnosis, Tongue Diseases parasitology
- Abstract
Leishmaniasis is a parasitic disease considered an endemic public health problem in developing countries, where it is a reportable disease. Isolated oral manifestation is rare, and its clinical manifestations are variable. In this paper we describe an unusual case of an immunocompetent patient, 57-year-old man with a painless reddish submucosal nodule located on the tongue dorsum. Microscopical analysis showed chronic inflammatory infiltrate with macrophages containing leishmania in cytoplasmic vacuoles. PCR assays confirmed the diagnosis and patient was treated with meglumine antimoniate for 30 days. Absence of the parasite was confirmed by PCR. Thirteen years after treatment, a scar fibrosis persisted on the tongue dorsum. The case reported reveals that leishmaniasis should be considered in the diagnosis of tongue nodules in immunocompetent patients., (© 2020. Springer Science+Business Media, LLC, part of Springer Nature.)
- Published
- 2021
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29. Beta-adrenergic pathway activation enhances aggressiveness and inhibits stemness in head and neck cancer.
- Author
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Lopes-Santos G, Bernabé DG, Miyahara GI, and Tjioe KC
- Abstract
Chronic stress leads to the activation of the beta-adrenergic pathway. Its activation has been implicated in the progression of different types of cancer but its role on head and neck squamous cell carcinomas (HNSCCs) remains undefined. The aim of this study was to investigate the influence of the beta-adrenergic pathway activation in the progression of HNSCCs and offer a panel of potential treatments for patients with the active beta-adrenergic pathway. Five hundred and twenty TCGA patients with primary HNSCCs were divided in two groups: ADRB2
low / SLC6A2low and ADRB2high / SLC6A2high . Differentially expressed genes (DEGs) were identified through differential expression analysis. The association of clinicopathological and genomic features between the groups was analyzed using a bioinformatic approach. Potential drugs for treatment of HNSCC were identified based on the DEGs. There was association between ADRB2 and SLC6A2 expressions with age, race, tumor site, histologic grade, perineural invasion, and HPV p16 status. It was identified 898 DEGs between the groups. High ADRB2/SLC6A2 expression stimulated HNSCC proliferation, adhesion, invasion, and angiogenesis. On the other hand, genes related to cell stemness were downregulated in patients with activation of the beta- adrenergic pathway. Finally, 56 FDA-approved antineoplastic and immunotherapeutic drugs were identified as potential targets for the personalized treatment., (Copyright © 2021. Published by Elsevier Inc.)- Published
- 2021
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30. Encephalopathy responsive to thiamine in severe COVID-19 patients.
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Branco de Oliveira MV, Irikura S, Lourenço FHB, Shinsato M, Irikura TCDB, Irikura RB, Albuquerque TVC, Shinsato VN, Orsatti VN, Fontanelli AM, Samegima DAG, Gonçalves MVM, and Bernabé DG
- Abstract
Encephalopathy is one of the most frequent neurological complications of severe Coronavirus Disease 2019 (COVID-19) patients. Cytokine storm and sepsis, hypercatabolic states, the use of furosemide and dialytic therapy represent risk factors for thiamine deficiency and are also found in patients with severe COVID-19. In this retrospective case series, we report clinical and neurological findings of fifteen patients with COVID-19-associated Wernicke Encephalopathy (WE) and their response to treatment with intravenous thiamine. All patients had encephalopathy, with 67% displaying at least one additional sign of classic WE triad (ophthalmoparesis and ataxia). Two patients (13%) had the classic triad. All COVID-19 patients had significant improvement of the neurological manifestations between two to five days after intravenous thiamine administration. Eleven patients (73%) had good neurological outcome at hospital discharge and only two patients (13%) died. This case series suggests that thiamine deficiency may be an etiology of encephalopathy in severe COVID-19 patients and its treatment may represent a safety and low-cost response to reduce the neurological burden., Competing Interests: None., (© 2021 The Authors.)
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- 2021
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31. Absence of human papillomavirus in cancer of the oral cavity and oropharynx in a Brazilian population.
- Author
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Santos IDS, Ferreira LL, Tomo S, Oliveira SHP, Biasoli ER, Bernabé DG, Villa LL, and Miyahara GI
- Subjects
- Brazil epidemiology, Humans, Mouth, Papillomaviridae, Alphapapillomavirus, Oropharyngeal Neoplasms epidemiology, Papillomavirus Infections diagnosis, Papillomavirus Infections epidemiology
- Published
- 2021
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32. In situ melanoma of oral cavity: Diagnosis and treatment of a rare entity.
- Author
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Cardoso DM, Bastos DB, Dos Santos DM, Conrado-Neto S, Collado FU, Crivelini MM, Xavier-Júnior JCC, Biasoli ÉR, Miyahara GI, and Bernabé DG
- Subjects
- Aged, Female, Humans, Melanoma pathology, Melanoma diagnosis, Melanoma therapy, Mouth Mucosa pathology
- Abstract
Oral melanoma is an extremely aggressive and rare tumor. Commonly, oral melanomas are diagnosed as invasive tumors, which considerably reduces the chances of cure. In situ oral melanomas being exceedingly rare, which makes its clinicopathological and prognostic characteristics poorly known. Herein, we report a case of 67-year-old non-white woman with a large black patch on the maxillary alveolar mucosa. A biopsy was made and microscopical analysis revealed moderate atypical junctional melanocytic. Tumor cells were positive for S100 (Polyclonal), Melan-A (Clone A103) and Melanosome (HMB-45). The diagnosis of in situ oral melanoma was made and the patient was treated surgically with partial maxillectomy and rehabilitated with obturator prosthesis. Although extremely rare in situ melanomas should be considered in the differential diagnosis of non-invasive pigmented lesions of the oral mucosa., (Copyright © 2020 Elsevier Ltd. All rights reserved.)
- Published
- 2021
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33. Granulomatous ulceration of the palate.
- Author
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de Castro TF, Tomo S, Santos-Silva AR, Bernabé DG, Biasoli ÉR, Crivelini MM, Okamoto AC, and Miyahara GI
- Subjects
- Humans, Palate, Ulcer
- Published
- 2021
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34. Wernicke Encephalopathy in COVID-19 Patients: Report of Three Cases.
- Author
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Branco de Oliveira MV, Bernabé DG, Irikura S, Irikura RB, Fontanelli AM, and Gonçalves MVM
- Abstract
Encephalopathy has been frequently reported in patients with acute respiratory distress syndrome (ARDS) related to COVID-19, and its etiology remains undetermined. These patients display hypercatabolic state, weight loss, use of diuretics, and dialytic therapy, which represent risk factors for thiamine depletion. The diagnosis of Wernicke encephalopathy (WE) is challenging and based on risk factors for the depletion of thiamine. We reported three cases of patients with COVID-19-related WE treated with intravenous thiamine. All patients responded with immediate neurologic improvement, and two of them had accelerated ventilatory weaning. The cases reported suggest that thiamine deficiency could represent relevant etiology of COVID-19-related encephalopathy., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Branco de Oliveira, Bernabé, Irikura, Irikura, Fontanelli and Gonçalves.)
- Published
- 2021
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35. Social isolation stress facilitates chemically induced oral carcinogenesis.
- Author
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Verza FA, Valente VB, Oliveira LK, Kayahara GM, Crivelini MM, Furuse C, Biasoli ÉR, Miyahara GI, Oliveira SHP, and Bernabé DG
- Subjects
- Animals, Cytokines metabolism, Inflammation Mediators metabolism, Male, Neoplasm Proteins metabolism, Rats, Rats, Wistar, 4-Nitroquinoline-1-oxide toxicity, Behavior, Animal, Depression metabolism, Depression pathology, Depression physiopathology, Head and Neck Neoplasms chemically induced, Head and Neck Neoplasms metabolism, Head and Neck Neoplasms pathology, Head and Neck Neoplasms physiopathology, Social Isolation, Squamous Cell Carcinoma of Head and Neck chemically induced, Squamous Cell Carcinoma of Head and Neck metabolism, Squamous Cell Carcinoma of Head and Neck pathology, Squamous Cell Carcinoma of Head and Neck physiopathology, Stress, Psychological metabolism, Stress, Psychological pathology, Stress, Psychological physiopathology
- Abstract
Social isolation has affected a large number of people and may lead to impairment of physical and mental health. Although stress resulting from social isolation may increase cancer progression, its interference on tumorigenesis is poorly known. In this study, we used a preclinical model to evaluate the effects of social isolation stress on chemically induced oral carcinogenesis. Sixty-two 21-day-old male Wistar rats were divided into isolated and grouped groups. After 90 days of age, the rats from both groups underwent oral carcinogenesis with 4-nitroquinoline 1-oxide (4NQO) for 20 weeks. All rats were assessed for depressive-like behavior and euthanized for oral squamous cell carcinoma (OSCC) diagnosis and measurement of inflammatory mediators in the tumor microenvironment. Social isolation stress increased the OSCC occurrence by 20.4% when compared to control. Isolated rats also showed higher tumor volume and cachexia than the grouped rats. Social isolation did not induce changes in the depressive-like behavior after carcinogenic induction. Tumors from stressed rats had increased levels of the inflammatory mediators, TNF-alpha, IL1-beta and MCP-1. The concentrations of TNF-alpha and MCP-1 were significantly increased in the large tumors from isolated animals. Higher tumor levels of TNF-alpha, IL-6, IL1-beta and MCP-1 were positively correlated with OSCC growth. This study provides the first evidence that social isolation stress may facilitate OSCC occurrence and tumor progression, an event accompanied by increased local levels of inflammatory mediators., Competing Interests: The authors have declared that no competing interests exist.
- Published
- 2021
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36. Beta-adrenergic blocker inhibits oral carcinogenesis and reduces tumor invasion.
- Author
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Cecilio HP, Valente VB, Pereira KM, Kayahara GM, Furuse C, Biasoli ÉR, Miyahara GI, Oliveira SHP, and Bernabé DG
- Subjects
- 4-Nitroquinoline-1-oxide administration & dosage, 4-Nitroquinoline-1-oxide toxicity, Animals, Carcinogenesis chemically induced, Carcinogens administration & dosage, Carcinogens toxicity, Cytokines immunology, Cytokines metabolism, Disease Progression, Humans, Male, Mouth Mucosa drug effects, Mouth Mucosa immunology, Mouth Mucosa pathology, Mouth Neoplasms chemically induced, Mouth Neoplasms pathology, Mouth Neoplasms prevention & control, Neoplasm Invasiveness prevention & control, Neoplasms, Experimental chemically induced, Neoplasms, Experimental pathology, Neoplasms, Experimental prevention & control, Rats, Rats, Wistar, Receptors, Adrenergic, beta metabolism, Signal Transduction drug effects, Squamous Cell Carcinoma of Head and Neck chemically induced, Squamous Cell Carcinoma of Head and Neck pathology, Squamous Cell Carcinoma of Head and Neck prevention & control, Tumor Microenvironment drug effects, Tumor Microenvironment immunology, Adrenergic beta-Antagonists administration & dosage, Carcinogenesis drug effects, Mouth Neoplasms drug therapy, Neoplasms, Experimental drug therapy, Propranolol administration & dosage, Squamous Cell Carcinoma of Head and Neck drug therapy
- Abstract
Purpose: Beta-adrenergic signaling can influence cancer progression and the use of beta blockers as adjuvant drugs in oncologic patients has been suggested. However, the involvement of beta-adrenergic blockers in tumorigenesis is poorly understood. This study investigated the action of beta-adrenergic blocker propranolol on tumor onset using a preclinical model of chemically induced oral cancer., Methods: Thirty-two male Wistar rats were subjected to daily subcutaneous injection of beta-blocker propranolol (10 mg/kg; SubQ), while another 32 rats received only a PBS injection (sham group). One week after starting propranolol treatment, all rats were submitted to chemical induction of oral carcinogenesis with 4-nitroquinoline-1-oxide (4NQO). After 16 weeks, they were assessed for occurrence of oral squamous cell carcinoma (OSCC), in addition to measurement of tumor volume and thickness, and tissue levels of cytokines IL-6, TNF-alpha and IL-10 in the tumor microenvironment., Results: Propranolol treatment reduced the occurrence of OSCC by 31%, 95% CI ( - 127, 216). Beta-adrenergic blocker significantly decreased thickness of OSCC when compared with PBS. Rats treated with propranolol exhibited a lower tumor volume when compared with control rats, but this result did not reach statistical significance. Tumors from propranolol-treated rats exhibited reduced concentrations of pro-inflammatory cytokines IL-6 and TNF-α. There was no difference in the IL-10 levels between tumors from propranolol- and sham-treated rats., Conclusion: Beta-adrenergic signaling may be one of the mechanisms associated with chemically induced oral carcinogenesis.
- Published
- 2020
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37. High p16 INK4a immunoexpression is not HPV dependent in oral leukoplakia.
- Author
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Tomo S, Biss SP, Crivelini MM, de Oliveira SHP, Biasoli ÉR, Tjioe KC, Bernabé DG, Villa LL, and Miyahara GI
- Subjects
- Adult, Aged, Aged, 80 and over, Biomarkers, Tumor, Brazil, Female, Humans, Male, Middle Aged, Young Adult, Carcinoma, Squamous Cell, Cyclin-Dependent Kinase Inhibitor p16 metabolism, Head and Neck Neoplasms, Leukoplakia, Oral metabolism, Papillomavirus Infections
- Abstract
Objective: To evaluate the prevalence of HPV DNA detection in fresh tissue from oral leukoplakia by Linear Array assay, and its correlation with p16
INK4a immunoexpression in the northwest region of the São Paulo state, Brazil., Patients and Methods: Fifty patients diagnosed with oral leukoplakia were included in the study. Sociodemographic, clinicopathologic and lifestyle data, fresh tissue and formalin fixed paraffin embedded (FFPE) tissue samples were collected. The fresh tissue was stored at -80 °C and then submitted to further viral DNA detection by the Linear Array method. Immunohistochemical analysis for the p16INK4a expression was performed., Results: Of the 50 patients included in the study, 62 % were men, and the age ranged from 25 to 82 years. Twenty-three (46 %) were elderly, 46 % were middle-aged adults, and only 12 % were young adults. Most patients were smokers (76 %), 14 % were former smokers, and 10 % were non-smokers. Most patients (56 %) were current drinkers, 22 % were ex-drinkers and 22 % were non-drinkers. Thirty-two percent of the lesions presented some degree of dysplasia. No lesions were positive to HPV by Linear Array detection. Thirty (60 %) OL had p16-low immunoexpression and 20 (40 %) had p16-high immunoexpression., Conclusion: HPV was not identified in the population studied. The high p16INK4a immunoexpression is not dependent on HPV in oral leukoplakia. Broader epidemiological studies are required to clarify the geographic variability in the prevalence of HPV in head and neck squamous cell carcinoma and oral potentially malignant lesions., Competing Interests: Declaration of Competing Interest The authors declare that there is no potential conflict of interest regarding this work., (Copyright © 2020 Elsevier Ltd. All rights reserved.)- Published
- 2020
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38. Diagnosis and Treatment of Oral Venous Malformation in a Child.
- Author
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Valente VB, Kayahara GM, Miyahara GI, Biasoli ÉR, and Bernabé DG
- Subjects
- Angiography, Child, Female, Humans, Injections, Intralesional, Mepivacaine therapeutic use, Oleic Acids therapeutic use, Sclerosing Solutions therapeutic use, Sclerotherapy, Vascular Malformations diagnostic imaging, Vascular Malformations therapy
- Abstract
Venous malformations (VMs) are congenital disorders that constitute about 40% of all vascular anomalies. These lesions do not regress spontaneously and may increase in size during childhood. The case of a 10-year-old girl with an extensive oral VM is reported. Intraoral examination revealed the presence of purplish nodules in the alveolar mucosa and gingiva from anterior maxilla. Doppler ultrasound showed a well-defined hypoechoic image and increased vascularization with low blood flow for the alveolar mucosa lesion. The patient was submitted to intralesional injections of the ethanolamine oleate/mepivacaine sclerosing solution. After four sessions, there was a significant reduction of the lesions. However, the patient abandoned the treatment and the oral VM grew progressively. After 1 year, sclerotherapy was resumed and performed weekly. After 10 session of sclerotherapy, the oral VM totally regressed. The childhood is a critical period for oral VM growth. Doppler ultrasound and sclerotherapy can be effective for the management of extensive lesions in children.
- Published
- 2020
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39. Pineal gland protects against chemically induced oral carcinogenesis and inhibits tumor progression in rats.
- Author
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Kayahara GM, Valente VB, Pereira RB, Lopes FYK, Crivelini MM, Miyahara GI, Biasoli ÉR, Oliveira SHP, and Bernabé DG
- Abstract
Clinical investigations suggest that melatonin suppression and circadian dysfunction may be related to cancer development in shift workers. Studies also show that melatonin suppression after pinealectomy increases cancer incidence in preclinical models. However, no study evaluated the influence of pinealectomy on oral cancer development. In the current study, we investigated the effects of pinealectomy on oral squamous cell carcinoma (OSCC) occurrence and progression in rats. Rats submitted to sham surgery were used as control. Pinealectomy promoted an increase of 140% in OSCC occurrence when compared to sham animals. Tumors from pinealectomized rats displayed a higher volume and thickness than the tumors from sham-operated animals. Pinealectomy induced atrophy of the epithelium adjacent to the oral lesions. Pinealectomized rats showed higher mean number of tumor-associated macrophages and eosinophils in the invasive front of OSCC. In addition, nuclear overexpression of ERK1/2 and p53 was also observed in the front of carcinomas from pinealectomized rats. These results reveal that pineal gland plays a protective role against oral carcinogenesis. The melatonin suppression caused by the pinealectomy might contribute to oral cancer development by acting on ERK1/2 and p53 pathways and regulating tumor inflammation., Competing Interests: CONFLICTS OF INTEREST None.
- Published
- 2020
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40. Benign symmetric lipomatosis in the tongue: an uncommon case.
- Author
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Bastos DB, Figueira JA, Furuse C, Biasoli ÉR, Miyahara GI, and Bernabé DG
- Subjects
- Aged, 80 and over, Biopsy, Diagnosis, Differential, Humans, Lipomatosis diagnosis, Lipomatosis, Multiple Symmetrical diagnosis, Male, Tongue pathology, Lipomatosis pathology, Tongue Neoplasms pathology
- Abstract
Symmetric lipomatosis of the tongue (SLT) is rare and characterized by diffuse growth and unencapsulated lipomas. An 87-year-old man was referred for evaluation of tongue lesions. Intraoral findings showed soft yellowish nodules with a smooth shiny surface diffusely on the lateral border of the tongue, bilaterally. Our clinical diagnosis was multiple tongue lipomas and an incisional biopsy was done. Histopathological examination revealed unencapsulated lobules of mature adipocytes with slight variation in the size and shape, confirming the diagnosis of lipoma. The final diagnosis was SLT. On follow-up at 6 months, the tongue findings were unchanged and no new lesions were observed.
- Published
- 2020
41. Childhood trauma is predictive for clinical staging, alcohol consumption, and emotional symptoms in patients with head and neck cancer.
- Author
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Sarafim-Silva BAM, Duarte GD, Sundefeld MLMM, Biasoli ÉR, Miyahara GI, and Bernabé DG
- Subjects
- Adolescent, Adult, Aged, Aged, 80 and over, Alcoholism epidemiology, Alcoholism psychology, Anxiety diagnosis, Brazil epidemiology, Child, Child Abuse psychology, Child, Preschool, Depression diagnosis, Female, Humans, Infant, Infant, Newborn, Life Change Events, Male, Middle Aged, Neoplasm Staging, Prognosis, Young Adult, Alcohol Drinking epidemiology, Anxiety epidemiology, Child Abuse statistics & numerical data, Depression epidemiology, Emotions, Head and Neck Neoplasms diagnosis, Head and Neck Neoplasms epidemiology, Head and Neck Neoplasms pathology, Head and Neck Neoplasms psychology, Squamous Cell Carcinoma of Head and Neck diagnosis, Squamous Cell Carcinoma of Head and Neck epidemiology, Squamous Cell Carcinoma of Head and Neck pathology, Squamous Cell Carcinoma of Head and Neck psychology
- Abstract
Background: Traumatic events in childhood have been associated with the occurrence of anxiety and depression in adulthood. This relation has been investigated in patients with breast cancer; however, it has been little explored in patients with other types of cancer. The objective of this study was to evaluate the occurrence of childhood trauma in patients with head and neck cancer and its association with clinicopathological variables and anxiety and depression levels., Methods: The study included 110 patients with head and neck squamous cell carcinoma (HNSCC) before they started cancer treatment. Clinicopathological and biobehavioral data were collected from patients' medical records. Anxiety and depression levels were assessed with the Beck Anxiety Inventory and the Beck Depression Inventory, respectively. The Childhood Trauma Questionnaire was used to evaluate the occurrence of traumatic events in childhood., Results: One hundred five patients (95.5%) experienced at least 1 type of childhood trauma. Emotional neglect was the most reported childhood trauma (43.8%), and multiple regression revealed that it was an independent variable for advanced clinical staging (β = 2.15, P = .048) and higher alcohol consumption (β = 2.32, P = .031). Patients with HNSCC who experienced more traumatic events in childhood had an almost 12 times greater chance of increased depression levels during the pretreatment period (β = 11.89; P = .0002). The occurrence of physical child neglect was a predictive factor for increased anxiety levels (β = 4.17, P = 0.029)., Conclusions: Traumatic events in childhood are predictive for advanced clinical staging, alcohol consumption, and emotional symptoms in patients with HNSCC, and they should be considered in clinical and psychological intervention strategies during cancer treatment. Cancer 2018;000:000-000. © 2018 American Cancer Society., (© 2018 American Cancer Society.)
- Published
- 2018
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42. Circulating catecholamines are associated with biobehavioral factors and anxiety symptoms in head and neck cancer patients.
- Author
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Bastos DB, Sarafim-Silva BAM, Sundefeld MLMM, Ribeiro AA, Brandão JDP, Biasoli ÉR, Miyahara GI, Casarini DE, and Bernabé DG
- Subjects
- Adult, Aged, Anxiety complications, Anxiety pathology, Epinephrine blood, Female, Humans, Leukoplakia, Oral pathology, Male, Middle Aged, Norepinephrine blood, Squamous Cell Carcinoma of Head and Neck complications, Squamous Cell Carcinoma of Head and Neck pathology, Sympathetic Nervous System pathology, Tremor blood, Tremor physiopathology, Anxiety blood, Catecholamines blood, Leukoplakia, Oral blood, Squamous Cell Carcinoma of Head and Neck blood
- Abstract
Studies have shown that stress-related catecholamines may affect cancer progression. However, little is known about catecholamine secretion profiles in head and neck cancer patients. The present study investigated plasma norepinephrine and epinephrine levels in head and neck squamous cell carcinoma (HNSCC) patients and patients with oral leukoplakia, as well as their association with clinicopathological and biobehavioral variables and anxiety symptoms. A total of 93 patients with HNSCC and 32 patients with oral leukoplakia were included. Plasma norepinephrine and epinephrine levels were measured by high performance liquid chromatography with electrochemical detection (HPLC-ED), and psychological anxiety levels were measured by the Beck Anxiety Inventory (BAI). Plasma norepinephrine and epinephrine concentrations were significantly higher in patients with oral and oropharyngeal squamous cell carcinoma (SCC) compared to non-cancer patients. Oral SCC patients displayed plasma norepinephrine levels about six times higher than oropharyngeal SCC patients, and nine times higher than oral leukoplakia patients (p < .001). Plasma epinephrine levels in oral SCC patients were higher compared to the oropharyngeal SCC (p = .0097) and leukoplakia (p < .0001) patients. Oropharyngeal SCC patients had higher plasma norepinephrine (p = .0382) and epinephrine levels (p = .045) than patients with oral leukoplakia. Multiple regression analyses showed that a history of high alcohol consumption was predictive for reduced plasma norepinephrine levels in the oral SCC group (p < .001). Anxiety symptom of "hand tremor" measured by the BAI was an independent predictor for higher plasma norepinephrine levels in HNSCC patients (β = 157.5, p = .0377), while the "heart pounding/racing" symptom was independently associated with higher plasma epinephrine levels in the oropharyngeal SCC group (β = 15.8, p = .0441). In oral leukoplakia patients, sleep deprivation and worse sleep quality were independent predictors for higher plasma norepinephrine levels, while severe tobacco consumption and higher anxiety levels were factors for higher plasma epinephrine levels. These findings suggest that head and neck cancer patients display sympathetic nervous system hyperactivity, and that changes in circulating catecholamines may be associated with alcohol consumption, as well as withdrawal-related anxiety symptoms., Competing Interests: The authors have declared that no competing interests exist.
- Published
- 2018
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43. Stress hormones concentrations in the normal microenvironment predict risk for chemically induced cancer in rats.
- Author
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Valente VB, Verza FA, Lopes FYK, Ferreira JZ, Dos Santos PSP, Sundefeld MLMM, Biasoli ÉR, Miyahara GI, Soubhia AMP, de Andrade M, de Oliveira SHP, and Bernabé DG
- Subjects
- 4-Nitroquinoline-1-oxide pharmacology, Adrenocorticotropic Hormone, Animals, Biomarkers, Tumor, Brain-Derived Neurotrophic Factor, Carcinogenesis metabolism, Carcinogens, Cellular Microenvironment physiology, Corticosterone, Disease Models, Animal, Male, Neoplasms chemically induced, Neoplasms metabolism, Norepinephrine, Rats, Rats, Wistar, Risk Factors, Tongue Neoplasms chemically induced, Hormones metabolism, Tongue metabolism, Tongue Neoplasms metabolism
- Abstract
Evidence show that stress hormones can influence cancer progression, but its role in carcinogenesis is poorly understood. In this study, we used a new method based on oral carcinogenesis model in rats to test the hypothesis that physiological levels of stress hormones in the normal tissue microenvironment would have significant predictive value for chemically induced cancer occurrence. Male Wistar rats were submitted to a tongue biopsy for measuring not-stress induced levels of norepinephrine, corticosterone, adrenocorticotropic hormone (ACTH) and brain-derived neurotrophic factor (BDNF) in the tissue before carcinogenic induction. Rats were treated with the 4-nitroquinoline-1-oxide (4NQO) chemical carcinogen for twenty weeks and then euthanized for microscopic evaluation of the tongue lesions. Increased pre-carcinogen norepinephrine concentrations and reduced basal corticosterone levels in the normal tissue microenvironment were predictive for oral squamous cell carcinoma (OSCC) occurrence. Likewise, increased pre-carcinogen norepinephrine levels in the normal microenvironment were associated a lower expression of pCDKN2a-p16 in OSCCs. Post-carcinogen levels of corticosterone and BDNF in oral leukoplakia tissues (precursor lesion of OSCC) and post-carcinogen corticosterone concentrations in OSCCs were higher than basal levels in the normal mucosa. Increased norepinephrine concentrations in OSCCs were associated to a greater tumor volume and thickness. Furthermore, higher levels of norepinephrine, ACTH and BDNF in OSCCs were associated to a lesser intensity of the lymphoplasmocytic infiltrate. This study shows that pre-carcinogen stress hormones levels in the normal microenvironment may be predictive for chemically induced cancer in rats. Moreover, chemical carcinogenesis can promote stressor-like effects with hormonal changes in the tissue microenvironment, which may be associated to tumor progression., (Copyright © 2017 Elsevier Ltd. All rights reserved.)
- Published
- 2018
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44. Alterations in opioid inhibition cause widespread nociception but do not affect anxiety-like behavior in oral cancer mice.
- Author
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Ye Y, Bernabé DG, Salvo E, Viet CT, Ono K, Dolan JC, Janal M, Aouizerat BE, Miaskowski C, and Schmidt BL
- Subjects
- Animals, Anxiety etiology, Cancer Pain etiology, Carcinoma, Squamous Cell metabolism, Cell Line, Tumor, Female, Head and Neck Neoplasms metabolism, Heterografts, Humans, Mice, Mice, Inbred BALB C, Spinal Cord metabolism, Squamous Cell Carcinoma of Head and Neck, Tongue Neoplasms metabolism, Anxiety metabolism, Cancer Pain metabolism, Carcinoma, Squamous Cell complications, Head and Neck Neoplasms complications, Nociceptive Pain metabolism, Receptors, Opioid biosynthesis, Tongue Neoplasms complications
- Abstract
Widespread pain and anxiety are commonly reported in cancer patients. We hypothesize that cancer is accompanied by attenuation of endogenous opioid-mediated inhibition, which subsequently causes widespread pain and anxiety. To test this hypothesis we used a mouse model of oral squamous cell carcinoma (SCC) in the tongue. We found that mice with tongue SCC exhibited widespread nociceptive behaviors in addition to behaviors associated with local nociception that we reported previously. Tongue SCC mice exhibited a pattern of reduced opioid receptor expression in the spinal cord; intrathecal administration of respective mu (MOR), delta (DOR), and kappa (KOR) opioid receptor agonists reduced widespread nociception in mice, except for the fail flick assay following administration of the MOR agonist. We infer from these findings that opioid receptors contribute to widespread nociception in oral cancer mice. Despite significant nociception, mice with tongue SCC did not differ from sham mice in anxiety-like behaviors as measured by the open field assay and elevated maze. No significant differences in c-Fos staining were found in anxiety-associated brain regions in cancer relative to control mice. No correlation was found between nociceptive and anxiety-like behaviors. Moreover, opioid receptor agonists did not yield a statistically significant effect on behaviors measured in the open field and elevated maze in cancer mice. Lastly, we used an acute cancer pain model (injection of cancer supernatant into the mouse tongue) to test whether adaptation to chronic pain is responsible for the absence of greater anxiety-like behavior in cancer mice. No changes in anxiety-like behavior were observed in mice with acute cancer pain., (Copyright © 2017 IBRO. Published by Elsevier Ltd. All rights reserved.)
- Published
- 2017
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45. Oral ulcers associated with bone destruction as the primary manifestation of histoplasmosis in an immunocompetent patient.
- Author
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Figueira JA, Camilo Júnior D, Biasoli ÉR, Miyahara GI, and Bernabé DG
- Subjects
- Antifungal Agents therapeutic use, Biopsy, Histoplasma, Histoplasmosis drug therapy, Humans, Immunocompetence, Itraconazole therapeutic use, Jaw Diseases drug therapy, Male, Middle Aged, Oral Ulcer drug therapy, Radiography, Panoramic, Histoplasmosis microbiology, Jaw Diseases microbiology, Oral Ulcer microbiology
- Published
- 2017
- Full Text
- View/download PDF
46. Uncommon oral manifestation of lichen sclerosus: critical analysis of cases reported from 1957 to 2016.
- Author
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Tomo S, Santos IS, de Queiroz SA, Bernabé DG, Simonato LE, and Miyahara GI
- Subjects
- Adolescent, Adult, Aged, Child, Female, Humans, Male, Middle Aged, Time Factors, Young Adult, Lichen Sclerosus et Atrophicus complications, Mouth Diseases etiology
- Abstract
Background: Lichen sclerosus is a mucocutaneous autoimmune disease which might be initiated by infectious pathogens as Borrelia Bugrdorferi and HPV. This disease shows destructive potential and is rarely diagnosed in oral mucosa. The purpose of this paper is to evaluate the characteristics of cases described in literature from 1957 to 2016, looking to provide valuable evidence about clinicopathologic features of this disease., Material and Methods: A MedLine search was performed aiming to find oral lichen sclerosus cases in literature and discuss its demographical and pathological characteristics as well as treatment methods performed for these cases., Results: 34 oral lichen sclerosus cases with histological confirmation and one clinicopathologic study linked with this disease were found in literature. Oral lichen sclerosus affected most commonly female patients, were asymptomatic and not associated to skin or genital lesions. Furthermore, affected patients in a range of 7 - 70-years old (Average age = 31.81)., Conclusions: Oral lichen sclerosus is a rare pathologic process with slight predilection for prepubertal girls, for which topical corticosterois have demonstrated satisfactory therapeutic value.
- Published
- 2017
- Full Text
- View/download PDF
47. Plasma HPV DNA is detectable in oral leukoplakia patients.
- Author
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Ferreira LL, Biasoli ÉR, Bernabé DG, Nunes CM, and Miyahara GI
- Subjects
- Adult, Aged, Biopsy, Case-Control Studies, DNA, Viral blood, DNA, Viral isolation & purification, Female, Humans, Leukoplakia, Oral blood, Leukoplakia, Oral pathology, Male, Middle Aged, Papillomaviridae isolation & purification, Papillomavirus Infections blood, Papillomavirus Infections diagnosis, Polymerase Chain Reaction, Predictive Value of Tests, Saliva virology, DNA, Viral genetics, Human Papillomavirus DNA Tests, Leukoplakia, Oral virology, Papillomaviridae genetics, Papillomavirus Infections virology
- Abstract
Background: Oral leukoplakia is considered a potentially malignant lesion for the development of squamous cell carcinoma, and various risk factors may be related to its development and malignant transformation, including the human papillomavirus (HPV). The aim of this case-control study was to detect the presence of HPV in fresh tissue, plasma and saliva samples obtained from patients with and without oral leukoplakia, and verify the correlation of the presence of DNA of HPV between different sources of materials., Methods: In this study, 32 patients with oral leukoplakia and 24 patients selected in a case-control manner were included. DNA extraction from the samples was performed, and afterwards it was amplified by nested polymerase chain reaction (nPCR) for the detection of HPV (nPCR: MY09-MY11/GP05+-GP06+)., Results: The DNA of HPV was found in 68.75% of the fresh tissue samples; in 50% of plasma, and in 62.5% of saliva samples in the group of patients with leukoplakia; in comparison with 45.8%, 54%, and 45.8%, in the fresh tissue, plasma and saliva samples, respectively, in the control group., Conclusion: Based on the present study, there was no difference in the rate of HPV detection in patients with or without oral leukoplakia. However all sources tested in this study were considered suitable for HPV detection, especially plasma samples, which showed be an important non-invasive source of HPV detection in leukoplakia patients., (Copyright © 2017 Elsevier GmbH. All rights reserved.)
- Published
- 2017
- Full Text
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48. Extra-Gingival Pyogenic Granuloma Displacing Teeth.
- Author
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Dal Prá KJ, Bernabé DG, Miyahara GI, Biasoli ER, Crivelini MM, and Tjioe KC
- Subjects
- Humans, Male, Middle Aged, Granuloma, Pyogenic complications, Lip Diseases complications, Tooth Migration etiology
- Abstract
Pyogenic granuloma is a common cause of growth of soft tissue in the oral cavity, especially in the gingiva. It is mainly associated with local and chronic irritants besides hormonal changes during pregnancy. Here, the authors present an unusual patient of an extra-gingival pyogenic granuloma with large dimensions and displacing teeth arising in a male patient. This is an interesting patient to be reported due to its exacerbated and atypical clinical features.
- Published
- 2017
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- View/download PDF
49. Lollipop-induced oral lichenoid reaction in a child.
- Author
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Bastos DB, Santos IS, Valente VB, Biel AC, Felipini RC, Biasoli ER, Miyahara GI, and Bernabé DG
- Subjects
- Administration, Topical, Adolescent, Biopsy, Diagnosis, Differential, Female, Glucocorticoids administration & dosage, Humans, Mouth Mucosa, Tongue, Candy adverse effects, Lichen Planus, Oral diagnosis
- Abstract
Background: Oral lichenoid reactions (OLRs) have been rarely reported in pediatric patients., Case Report: This article reports an unusual case of a 15-year-old girl who had white plaques in tongue and buccal mucosa oral which initially were diagnosed as oral lichen planus (OLP). At first, the patient and her family denied systemic or local medication use. Biopsy was performed, and histopathological aspects were suggestive of OLP. Despite the attempt of treatment with topical corticoid, the lesions did not recede. Patient's behavior and medical history were again investigated and revealed that she had been using almost daily in her school an artificially colored and flavored lollipop. One week after removing lollipop use behavior, the oral lesions showed complete resolution and OLR diagnosis was defined., Conclusion: The present case denotes that OLR should be considered in the differential diagnosis of oral white plates in children and artificially colored sweets can be its causal agent., (© 2016 BSPD, IAPD and John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
- Published
- 2016
- Full Text
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50. Pemphigus Vulgaris: How to Perform an Oral Biopsy Properly?
- Author
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Moraes da Silva AF, Bernabé DG, Miyahara GI, Biasoli ER, Callestini R, and Tjioe KC
- Subjects
- Adult, Biopsy, Female, Humans, Pemphigus diagnosis, Mouth Mucosa pathology, Pemphigus pathology
- Abstract
Oral biopsy of vesiculobullous diseases such as pemphigus vulgaris often raises questions due to some particularities involving this procedure. The adequate selection of the area to be biopsied defines if the final diagnosis will be reached, being the Achiles heel of the proper management of the patient. Here, the authors report a case of a woman who sought for treatment of generalized oral blisters and ulcers that caused severe pain. She had undergone a previous biopsy by other professionals that was inconclusive. The authors' team diagnosed the patient with pemphigus vulgaris and treated her properly. The authors provide a brief discussion about the adequate oral site to be chosen when dealing with vesiculobullous diseases once it still remains a source of doubts for the dental surgeons.
- Published
- 2016
- Full Text
- View/download PDF
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