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[ES] En el actual Trabajo Final de Grado (TFG) de la titulación de Grado en Ingeniería en tecnologías Industriales, se basa en un proyecto en el cual se ha colaborado y participado en el intervalo de tiempo de prácticas en la empresa Autis Ingenieros. El proyecto consta de dos partes: la primera el apilado de producto final en el muelle correspondiente y la segunda parte el transporte del producto final a la zona de clasificación. Solo se tratará la segunda parte del proyecto debido a la extensión y complicidad de realizar las dos partes. La parte que se va a tratar consiste en el diseño y elaboración del transporte y la clasificación del producto, este consiste en 3 cajas de encimera de gran calidad; el proyecto ha sido contratado por la empresa B/S/H en las instalaciones de la planta de Montañana en Zaragoza. El proyecto se compone de 2 robots móviles autónomos que irán a recoger el producto para luego ir a depositarlo al muelle de clasificación correspondiente. En el transporte el robot va a tener que ir evitando obstáculos y junto con el PLC irán comunicando para tener la información necesaria en todo momento. Para la realización del TFG se presentará la programación del PLC, correspondiente a un dispositivo de la marca siemens programado con el software TIA Portal V15.0. Este PLC será de seguridad para poder implementar todas las funciones de seguridad que componen nuestro proyecto. En la programación se explicará la realización de las funciones de control principal más importantes y las funciones seguridad. Posteriormente se centrará el foco en el robot autónomo y su programación. El robot será tipo AMR (Autonomous Mobile Robots), en este caso se utilizará uno de la marca MiR, para más detalle el MiR200. El robot se irá comunicando con el PLC mediante una conexión Modbus TCP/IP a través de tres puntos de acceso wifi repartidos por el área de trabajo, correspondiente a 3 zonas distanciadas de la fábrica por donde circulará el robot. También se hará especial hin, [EN] The current Final Degree Work (TFG) of the bachelor¿s degree in Industrial Technologies Engineering is focused on a project that has been developed during the Internship time at the Autis Ingenieros company. The project consists of two parts, in the first part the final product is stacked in the corresponding dock and the second part that consists in transporting the final product to the classification area. In this work it will only be treated the last part due to the complexity and extension of the performance of both procedures. This part is based on the design and elaboration of the transport and in the classification of the final product, which is composed by 3 boxes that contain one high quality hob each one. The project has been hired by the B/S/H company whose installations are located in Montañana (Zaragoza). The actions previously described will be performed by two autonomous mobile robots that during the transportation will have to avoid obstacles and joint with the PLC will have to be able to report the information required at any time of the transport. In the current TFG the PLC programming will be presented, which corresponds to a Siemens device programmed with the TIA Portal V15.0 software. The mentioned PLC will be a security PLC in order to be able to implement all the security functions that are required by the project. Moreover, an explanation of the realization of the main control and security functions will be provided in the programming. Afterwards we will focus on the autonomous robot and its programming. The robot will be AMR (Autonomous Mobile Robots) type; in this case the MiR200 will be used, which is provided by the brand MiR. The robot will be keeping in contact with the PLC via a connection Modbus TCP/IP trough three Wifi access points located over 3 areas of the plant where the robot will be operating. Special emphasis will also be placed on the parts where I have contributed to the development of the project., [CA] En l'actual Treball Final de Grau (TFG) de la titulació de Grau en Enginyeria en tecnologies industrials, es basa en un projecte en el qual s'ha col·laborat i participat en l'interval de temps de pràctiques a l'empresa Autis Enginyers. El projecte consta de dues parts: la primera l'apilament de producte final al moll corresponent i la segona part el transport del producte final a la zona de classificació. Només es tractarà la segona part de el projecte a causa de l'extensió i complicitat de realitzar les dues parts. La part que es va a tractar consisteix en el disseny i elaboració del transport i la classificació del producte, aquest consisteix en 3 caixes de taulell de gran qualitat; el projecte ha estat contractat per l'empresa B / S / H en les instal·lacions de la planta de Montanyana a Saragossa. El projecte es compon de 2 robots mòbils autònoms que aniran a recollir el producte per després anar a dipositar al moll de classificació corresponent. En el transport el robot va haver d'anar evitant obstacles i juntament amb el PLC aniran comunicant per tenir la informació necessària en tot moment. Per a la realització de l'TFG es presentarà la programació del PLC, corresponent a un dispositiu de la marca siemens programat amb el programari TIA Portal V15.0. Aquest PLC serà de seguretat per poder implementar totes les funcions de seguretat que componen el nostre projecte. En la programació s'explicarà la realització de les funcions de control principal més importants i les funcions seguretat. Posteriorment es centrarà el focus en el robot autònom i la seva programació. El robot serà tipus AMR (Autonomous Mobile Robots), en aquest cas s'utilitzarà un de la marca Mir, per a més detall el MiR200. El robot s'anirà comunicant amb el PLC mitjançant una connexió Modbus TCP / IP a través de tres punts d'accés wifi repartits per l'àrea de treball, corresponent a 3 zones distanciades de la fàbrica per on circularà el robot. També es farà especial èmfasi en les parts on m'h

Purpose: Non-small-cell lung cancers harboring EML4-ALK rearrangements are sensitive to crizotinib. However, despite initial response, most patients will eventually relapse, and monitoring EML4-ALK rearrangements over the course of treatment may help identify these patients. However, challenges associated with serial tumor biopsies have highlighted the need for blood-based assays for the monitoring of biomarkers. Platelets can sequester RNA released by tumor cells and are thus an attractive source for the non-invasive assessment of biomarkers. Methods: EML4-ALK rearrangements were analyzed by RT-PCR in platelets and plasma isolated from blood obtained from 77 patients with non-small-cell lung cancer, 38 of whom had EML4-ALK-rearranged tumors. In a subset of 29 patients with EML4-ALK-rearranged tumors who were treated with crizotinib, EML4-ALK rearrangements in platelets were correlated with progression-free and overall survival. Results: RT-PCR demonstrated 65% sensitivity and 100% specificity for the detection of EML4-ALK rearrangements in platelets. In the subset of 29 patients treated with crizotinib, progression-free survival was 3.7 months for patients with EML4-ALK+ platelets and 16 months for those with EML4-ALK- platelets (hazard ratio, 3.5; P = 0.02). Monitoring of EML4-ALK rearrangements in the platelets of one patient over a period of 30 months revealed crizotinib resistance two months prior to radiographic disease progression. Conclusions: Platelets are a valuable source for the non-invasive detection of EML4-ALK rearrangements and may prove useful for predicting and monitoring outcome to crizotinib, thereby improving clinical decisions based on radiographic imaging alone.

During cerebellum development, Sonic hedgehog (Shh)-induced proliferation of cerebellar granular neuronal precursors (CGNPs) is potently inhibited by bone morphogenetic proteins (BMPs). We have previously reported the upregulation of TIEG-1 and Mash1, two antimitotic factors that modulate MYCN transcription and N-Myc activity, in response to BMP2. To gain further insight into the BMP antimitotic mechanism, we used microRNA (miRNA) arrays to compare the miRNAs of CGNPs proliferating in response to Shh with those of CGNPs treated with Shh plus BMP2. The array analysis revealed that miRNA 11 (miR-22) levels significantly increased in cells treated with BMP2. Additionally, in P7 mouse cerebellum, miR-22 distribution mostly recapitulated the combination of BMP2 and BMP4 expression patterns. Accordingly, in CGNP cultures, miR-22 overexpression significantly reduced cell proliferation, whereas miR-22 suppression diminished BMP2 antiproliferative activity. In contrast to BMP2, miR-22 did not induce neural differentiation but instead significantly increased cell cycle length. Consistent with the central role played by N-myc on CGNP proliferation, Max was revealed as a direct target of miR-22, and miR-22 expression caused a significant reduction of Max protein levels and N-myc/Max-dependent promoter activity. Therefore, we conclude that, in addition to the previously described mechanisms, miR-22 plays a specific role on downstream BMPs through cerebellum growth

Cinnamyl alcohol dehydrogenase (CAD) is a key enzyme involved in the last step of monolignol biosynthesis. The effect of CAD down-regulation on lignin production was investigated through a transgenic approach in maize. Transgenic CAD-RNAi plants show a different degree of enzymatic reduction depending on the analyzed tissue and show alterations in cell wall composition. Cell walls of CAD-RNAi stems contain a lignin polymer with a slight reduction in the S-to-G ratio without affecting the total lignin content. In addition, these cell walls accumulate higher levels of cellulose and arabinoxylans. In contrast, cell walls of CAD-RNAi midribs present a reduction in the total lignin content and of cell wall polysaccharides. In vitro degradability assays showed that, although to a different extent, the changes induced by the repression of CAD activity produced midribs and stems more degradable than wild-type plants. CAD-RNAi plants grown in the field presented a wild-type phenotype and produced higher amounts of dry biomass. Cellulosic bioethanol assays revealed that CAD-RNAi biomass produced higher levels of ethanol compared to wild-type, making CAD a good target to improve both the nutritional and energetic values of maize lignocellulosic biomass.