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27 results on '"Berendzen, Kenneth Wayne"'

1. Cytokinins regulate spatially specific ethylene production to control root growth in Arabidopsis

Author

Yamoune, Amel, Zdarska, Marketa, Depaepe, Thomas, Rudolfova, Anna, Skalak, Jan, Berendzen, Kenneth Wayne, Mira-Rodado, Virtudes, Fitz, Michael, Pekarova, Blanka, Nicolas Mala, Katrina Leslie, Tarr, Paul, Spackova, Eliska, Tomovicova, Lucia, Parizkova, Barbora, Franczyk, Abigail, Kovacova, Ingrid, Dolgikh, Vladislav, Zemlyanskaya, Elena, Pernisova, Marketa, Novak, Ondrej, Meyerowitz, Elliot, Harter, Klaus, Van Der Straeten, Dominique, and Hejatko, Jan

Published
2024
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4. A tyrosine phospho‐switch within the Longin domain of VAMP721 modulates SNARE functionality

Author

Ricardi, Martiniano Maria, primary, Wallmeroth, Niklas, additional, Cermesoni, Cecilia, additional, Mehlhorn, Dietmar Gerald, additional, Richter, Sandra, additional, Zhang, Lei, additional, Mittendorf, Josephine, additional, Godehardt, Ingeborg, additional, Berendzen, Kenneth Wayne, additional, von Roepenack‐Lahaye, Edda, additional, Stierhof, York‐Dieter, additional, Lipka, Volker, additional, Jürgens, Gerd, additional, and Grefen, Christopher, additional

Published
2023
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6. Cytokinins regulate spatially-specific ethylene production to control root growth in Arabidopsis

Author

Yamoune, Amel, Zdarska, Marketa, Depaepe, Thomas, Korytarova, Anna, Skalak, Jan, Berendzen, Kenneth Wayne, Mira-Rodado, Virtudes, Tarr, Paul, Spackova, Eliska, Badurova, Lucia, Parizkova, Barbora, Franczyk, Abigail, Kovacova, Ingrid, Pernisova, Marketa, Novak, Ondrej, Meyerowitz, Elliot, Harter, Klaus, Van Der Straeten, Dominique, Hejátko, Jan, Yamoune, Amel, Zdarska, Marketa, Depaepe, Thomas, Korytarova, Anna, Skalak, Jan, Berendzen, Kenneth Wayne, Mira-Rodado, Virtudes, Tarr, Paul, Spackova, Eliska, Badurova, Lucia, Parizkova, Barbora, Franczyk, Abigail, Kovacova, Ingrid, Pernisova, Marketa, Novak, Ondrej, Meyerowitz, Elliot, Harter, Klaus, Van Der Straeten, Dominique, and Hejátko, Jan

Abstract

The two principal growth regulators cytokinins and ethylene are known to interact in the regulation of plant growth. However, information about underlying molecular mechanism and positional specificity of the cytokinin/ethylene crosstalk in root growth control is scarce. We have identified spatial specificity of cytokinin-regulated root elongation and root apical meristem (RAM) size, both of which we demonstrate to be ethylene biosynthesis-dependent. Upregulation of the cytokinin biosynthetic gene ISOPENTENYLTRANSFERASE(IPT) in proximal and peripheral tissues leads to both root and RAM shortening. In contrast, IPTactivation in distal and inner tissues reduces RAM size while leaving the root length comparable to mock-treated controls. We show that cytokinins regulate two steps specific to ethylene biosynthesis, the production of ACC by ACC SYNTHASEs (ACSs), and its conversion to ethylene by ACC OXIDASEs (ACOs). We describe cytokinin- and ethylene-specific regulation controlling the activity of ACSs and ACOs that are spatially discrete along both proximo/distal and radial root axes. Using direct ethylene measurements, we identifyACO2, ACO3andACO4as being responsible for ethylene biosynthesis and the ethylene-regulated root and RAM shortening in cytokinin-treated Arabidopsis. Finally, we describe the tight cooperation between cytokinin and ethylene signaling in cytokinin-induced, ethylene-regulated control ofACO4due to the direct interaction between ARABIDOPSIS RESPONSE REGULATOR 2 (ARR2), a member of the multistep phosphorelay cascade and the C-terminal portion of ETHYLENE INSENSITIVE 2 (EIN2-C), a key regulator of canonical ethylene signaling.

Published
2023

8. Cytokinins regulate spatially-specific ethylene production to control root growth inArabidopsis

Author

Yamoune, Amel, primary, Zdarska, Marketa, additional, Depaepe, Thomas, additional, Korytarova, Anna, additional, Skalak, Jan, additional, Berendzen, Kenneth Wayne, additional, Mira-Rodado, Virtudes, additional, Tarr, Paul, additional, Spackova, Eliska, additional, Badurova, Lucia, additional, Parizkova, Barbora, additional, Franczyk, Abigail, additional, Kovacova, Ingrid, additional, Pernisova, Marketa, additional, Novak, Ondrej, additional, Meyerowitz, Elliot, additional, Harter, Klaus, additional, Van Der Straeten, Dominique, additional, and Hejatko, Jan, additional

Published
2023
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12. The tomato resistance gene Bs4 suppresses leaf watersoaking phenotypes induced by AvrHah1 , a transcription activator‐like effector from tomato‐pathogenic xanthomonads

Author

Schenstnyi, Kyrylo, primary, Strauß, Annett, additional, Dressel, Angela, additional, Morbitzer, Robert, additional, Wunderlich, Markus, additional, Andrade, Ana Gabriela, additional, Phan, Trang‐Thi‐Thu, additional, Aguilera, Paloma de los Angeles, additional, Brancato, Caterina, additional, Berendzen, Kenneth Wayne, additional, and Lahaye, Thomas, additional

Published
2022
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16. Endoplasmic reticulum membrane receptors of the GET pathway are conserved throughout eukaryotes

Author

Asseck, Lisa Yasmin, primary, Mehlhorn, Dietmar Gerald, additional, Monroy, Jhon Rivera, additional, Ricardi, Martiniano Maria, additional, Breuninger, Holger, additional, Wallmeroth, Niklas, additional, Berendzen, Kenneth Wayne, additional, Nowrousian, Minou, additional, Xing, Shuping, additional, Schwappach, Blanche, additional, Bayer, Martin, additional, and Grefen, Christopher, additional

Published
2020
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19. Endoplasmic reticulum membrane receptors of the GET pathway are conserved throughout eukaryotes.

Author

Asseck, Lisa Yasmin, Mehlhorn, Dietmar Gerald, Monroy, Jhon Rivera, Ricardi, Martiniano Maria, Breuninger, Holger, Wallmeroth, Niklas, Berendzen, Kenneth Wayne, Nowrousian, Minou, Shuping Xing, Schwappach, Blanche, Bayer, Martin, and Grefen, Christopher

Subjects
ENDOPLASMIC reticulum, IMMOBILIZED proteins, MEMBRANE proteins, MASS analysis (Spectrometry), EUKARYOTES
Abstract

Type II tail-anchored (TA) membrane proteins are involved in diverse cellular processes, including protein translocation, vesicle trafficking, and apoptosis. They are characterized by a single C-terminal transmembrane domain that mediates posttranslational targeting and insertion into the endoplasmic reticulum (ER) via the Guided-Entry of TA proteins (GET) pathway. The GET system was originally described in mammals and yeast but was recently shown to be partially conserved in other eukaryotes, such as higher plants. A newly synthesized TA protein is shielded from the cytosol by a pretargeting complex and an ATPase that delivers the protein to the ER, where membrane receptors (Get1/WRB and Get2/CAML) facilitate insertion. In the model plant Arabidopsis thaliana, most components of the pathway were identified through in silico sequence comparison, however, a functional homolog of the coreceptor Get2/CAML remained elusive. We performed immunoprecipitation- mass spectrometry analysis to detect in vivo interactors of AtGET1 and identified a membrane protein of unknown function with low sequence homology but high structural homology to both yeast Get2 and mammalian CAML. The protein localizes to the ER membrane, coexpresses with AtGET1, and binds to Arabidopsis GET pathway components. While loss-of-function lines phenocopy the stunted root hair phenotype of other Atget lines, its heterologous expression together with the coreceptor AtGET1 rescues growth defects of get1get2 yeast. Ectopic expression of the cytosolic, positively charged N terminus is sufficient to block TA protein insertion in vitro. Our results collectively confirm that we have identified a plant-specific GET2 in Arabidopsis, and its sequence allows the analysis of cross-kingdom pathway conservation. [ABSTRACT FROM AUTHOR]

Published
2021
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27. Canalization of genome-wide transcriptional activity in Arabidopsis thalianaaccessions by MET1-dependent CG methylation

Author

Srikant, Thanvi, Yuan, Wei, Berendzen, Kenneth Wayne, Contreras-Garrido, Adrián, Drost, Hajk-Georg, Schwab, Rebecca, and Weigel, Detlef

Abstract

Background : Despite its conserved role on gene expression and transposable element (TE) silencing, genome-wide CG methylation differs substantially between wild Arabidopsis thalianaaccessions. Results: To test our hypothesis that global reduction of CG methylation would reduce epigenomic, transcriptomic, and phenotypic diversity in A. thalianaaccessions, we knock out MET1, which is required for CG methylation, in 18 early-flowering accessions. Homozygous met1mutants in all accessions suffer from common developmental defects such as dwarfism and delayed flowering, in addition to accession-specific abnormalities in rosette leaf architecture, silique morphology, and fertility. Integrated analysis of genome-wide methylation, chromatin accessibility, and transcriptomes confirms that MET1inactivation greatly reduces CG methylation and alters chromatin accessibility at thousands of loci. While the effects on TE activation are similarly drastic in all accessions, the quantitative effects on non-TE genes vary greatly. The global expression profiles of accessions become considerably more divergent from each other after genome-wide removal of CG methylation, although a few genes with diverse expression profiles across wild-type accessions tend to become more similar in mutants. Most differentially expressed genes do not exhibit altered chromatin accessibility or CG methylation in cis, suggesting that absence of MET1 can have profound indirect effects on gene expression and that these effects vary substantially between accessions. Conclusions: Systematic analysis of MET1 requirement in different A. thalianaaccessions reveals a dual role for CG methylation: for many genes, CG methylation appears to canalize expression levels, with methylation masking regulatory divergence. However, for a smaller subset of genes, CG methylation increases expression diversity beyond genetically encoded differences.

Published
2022
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