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4 results on '"Beomhee Kim"'

1. Vibrio sp. dhg as a platform for the biorefinery of brown macroalgae

Author

Hyun Gyu Lim, Dong Hun Kwak, Sungwoo Park, Sunghwa Woo, Jae-Seong Yang, Chae Won Kang, Beomhee Kim, Myung Hyun Noh, Sang Woo Seo, and Gyoo Yeol Jung

Subjects
Science
Abstract

Brown macroalgae is a good candidate feedstock for biorefinery, but the major carbohydrate alginate cannot be digested by current industrial microbes. Here, the authors isolate Vibrio sp. dhg and engineer it to produce value-added biochemicals from alginate using newly developed genetic tools. more...

Published
2019
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2. Synthetic biology for evolutionary engineering: from perturbation of genotype to acquisition of desired phenotype

Author

Jina Yang, Beomhee Kim, Gi Yeon Kim, Gyoo Yeol Jung, and Sang Woo Seo

Subjects
Synthetic biology, Evolutionary engineering, Biosensor, Cell factory, Directed evolution, Fuel, TP315-360, Biotechnology, TP248.13-248.65
Abstract

Abstract With the increased attention on bio-based industry, demands for techniques that enable fast and effective strain improvement have been dramatically increased. Evolutionary engineering, which is less dependent on biological information, has been applied to strain improvement. Currently, synthetic biology has made great innovations in evolutionary engineering, particularly in the development of synthetic tools for phenotypic perturbation. Furthermore, discovering biological parts with regulatory roles and devising novel genetic circuits have promoted high-throughput screening and selection. In this review, we first briefly explain basics of synthetic biology tools for mutagenesis and screening of improved variants, and then describe how these strategies have been improved and applied to phenotypic engineering. Evolutionary engineering using advanced synthetic biology tools will enable further innovation in phenotypic engineering through the development of novel genetic parts and assembly into well-designed logic circuits that perform complex tasks. more...

Published
2019
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3. Complete Genome Sequence of Lactic Acid Bacterium Pediococcus acidilactici Strain ATCC 8042, an Autolytic Anti-bacterial Peptidoglycan Hydrolase Producer

Author

Sungwoo Park, Sang Woo Seo, Jina Yang, Beomhee Kim, and Sung Won Cho

Subjects
0106 biological sciences, Biomedical Engineering, Bioengineering, 01 natural sciences, Applied Microbiology and Biotechnology, Genome, 03 medical and health sciences, chemistry.chemical_compound, symbols.namesake, 010608 biotechnology, 030304 developmental biology, Genetics, Whole genome sequencing, Sanger sequencing, 0303 health sciences, biology, Contig, Circular bacterial chromosome, Nucleic acid sequence, food and beverages, Pediococcus acidilactici, biology.organism_classification, chemistry, symbols, Peptidoglycan, Biotechnology
Abstract

Pediococcus acidilactici is a probiotic bacterium that is industrially utilized in the food industry and antibiotics development. Here, we determine the complete nucleotide sequence of the genome of Pediococcus acidilactici ATCC 8042. The genome was sequenced by the PacBio RSII to generate a single contig consisting of circular chromosome sequence. Illumina MiniSeq sequencing platform and Sanger sequencing method were additionally utilized to correct errors resulting from the long-read sequencing platform. The sequence consists of 2,009,598 bp with a G + C content of 42.1% and contains 1,865 protein-coding sequences. Based on the sequence information, we could confirm and predict the presence of four peptidoglycan hydrolases by HyPe software. This work, therefore, provides the complete genomic information of P. acidilactici ATCC 8042 with a profitable potential of genome-scale comprehension of anti-pathogenic activity, which can be applied in nutraceutical and pharmaceutical biotechnology field. more...

Published
2019
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4. Transcriptional Profiling of the Probiotic Escherichia coli Nissle 1917 Strain under Simulated Microgravity

Author

Sung Won Cho, Sungwoo Park, Jaewoo Yim, Yong Hee Han, Sang Woo Seo, and Beomhee Kim

Subjects
0301 basic medicine, Metal ion transport, E. coli Nissle 1917, 030106 microbiology, RNA-sequencing, Carbon starvation, medicine.disease_cause, Catalysis, Article, Ion Channels, Effective solution, law.invention, Inorganic Chemistry, lcsh:Chemistry, 03 medical and health sciences, Probiotic, law, Stress, Physiological, medicine, Escherichia coli, Microbiome, Physical and Theoretical Chemistry, Molecular Biology, lcsh:QH301-705.5, Spectroscopy, Chemistry, Weightlessness, Gene Expression Profiling, Organic Chemistry, Biofilm, Computational Biology, General Medicine, Gene Expression Regulation, Bacterial, microgravity, Carbon, Computer Science Applications, Cell biology, 030104 developmental biology, Simulated microgravity, probiotics, lcsh:Biology (General), lcsh:QD1-999, Metals, Transcriptome, Metabolic Networks and Pathways
Abstract

Long-term space missions affect the gut microbiome of astronauts, especially the viability of some pathogens. Probiotics may be an effective solution for the management of gut microbiomes, but there is a lack of studies regarding the physiology of probiotics in microgravity. Here, we investigated the effects of microgravity on the probiotic Escherichia coli Nissle 1917 (EcN) by comparing transcriptomic data during exponential and stationary growth phases under simulated microgravity and normal gravity. Microgravity conditions affected several physiological features of EcN, including its growth profile, biofilm formation, stress responses, metal ion transport/utilization, and response to carbon starvation. We found that some changes, such as decreased adhesion ability and acid resistance, may be disadvantageous to EcN relative to gut pathogens under microgravity, indicating the need to develop probiotics optimized for space flight. more...

Published
2020