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1. Molecular epidemiology of Candida africana isolates collected from vagina swabs in French Guiana.

Author

Bigot, Jeanne, Kalboussi, Yasmine, Bonkoto Nkoy, Yannick, Benmostefa, Alexis, Vellaissamy, Sandra, Benzerara, Laurent, Sainte-Rose, Vincent, Blanchet, Denis, Demar, Magalie, Guitard, Juliette, and Hennequin, Christophe

Abstract

Previous molecular studies have shown that Candida africana corresponds to the clade 13 of Candia albicans. It has been mostly involved in vulvovaginal candidiasis worldwide but few data exist in South America. The aim of our study was to investigate the prevalence of C. africana in women living in French Guiana. For this, we first set up a fluorescent-intercalating-dye-real time Polymerase Chain Reaction (PCR) targeting the hyphal wall protein 1 gene. The test was applied to 212 C. albicans isolates collected from May to August 2019 from vaginal swabs, allowing the identification of six women harboring C. africana (eight isolates). The in vitro susceptibility of these eight isolates to six antifungal drugs was also evaluated. No demographics or clinical-specific features could be demonstrated. Genetic diversity of those isolates was analyzed through multilocus sequence typing and showed that diploid sequence type 182 was predominant (n  = 6) and allowed the report of a new diploid sequence type. [ABSTRACT FROM AUTHOR]

Published
2024
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2. Influence of daily green tea infusions on hypercalciuric renal stone patients: biological and morpho-constitutional analysis

Author

Rode, Julie, Benzerara, Laurent, Bazin, Dominique, Dessombs, Arnaud, Letavernier, Emmanuel, Hoznek, Andras, Traxer, Olivier, Daudon, Michel, Haymann, Jean-Philippe, Service d'urologie [Mondor], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Henri Mondor-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Service d'Explorations fonctionnelles multidisciplinaires [CHU Tenon], CHU Tenon [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Spectroscopie, Modélisation, Interfaces pour L'Environnement et la Santé (LCMCP-SMiLES), Laboratoire de Chimie de la Matière Condensée de Paris (LCMCP), Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), and HAL-UPMC, Gestionnaire

Subjects
[SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract

International audience; Calcium stones contain predominantly calcium oxalate monohydrate (COM) and calcium oxalate dihydrate (COD). Catechins of green tea may prevent crystallization of calcium oxalate crystals but tea is also a source of oxalates. Here we evaluate the influence of regular daily green tea intake on stone risk factors in 273 hypercalciuric renal stone formers, their stone morphology and composition and a potential in vitro solubilizing effect of epigallocatechin (EGC) on calcium stones. Green tea ‘’drinkers’’(n=48) and ‘’non drinkers’’(n=225) were compared according to gender with biochemical analyses and stone composition performed by infrared spectroscopy (IRS) on 117 patients. Scanning electron microscopy (SEM) renal stone analysis was realized of 57 stones containing COD as major component in the two groups. Different criteria were collected including the presence of pores and their shape. In vitro SEM analysis were performed after incubation with different solutions containing either EDTA 0.1 M, green tea, EGC 10- 300μM and sodium citrate 10-300 mM. The groups were similar for 24 hours calciuria and bone remodeling biomarkers but a green tea diuretic effect was suggested. IRS analysis showed a significant higher prevalence of COD in female “drinkers” whereas COD was found in nuclei in male “drinkers”. SEM analysis revealed a higher prevalence of square pores detected in “drinkers” and similar holes at the surface of COD crystals after incubation with green tea solution, EGC and calcium chelators, EDTA and sodium citrate. Green tea through catechins has a pharmacological effect with no influence on stone risk factors but a significant change on calcium stones composition and structure.

Published
2016

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