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An important function of the neocortex is to compare sensory feedback stimuli with internal predictions of the outside world and evoke mismatch responses to deviations, thus allowing expectations to be updated. The mechanisms behind sensory feedback mismatch and prediction formation however remain unclear. Here we created a learned association of an auditory-tactile stimulus sequence in awake head-fixed mice, where sound predicted an up-coming whisker stimulus, and introduced mismatches by omitting or altering the whisker stimulus intensity. We show that layer 2/3 posterior parietal cortex (PPC) neurons can report stimulus sequence mismatches, as well as display neural correlates of expectation which can be prolonged temporally. Inhibition of PPC-projecting secondary motor cortex M2 neurons suppressed these correlates, along with population mismatch responses, while conversely enhancing sensory processing. Hence, M2 can influence sensory processing in the PPC and potentially provide the prediction of sensory feedback from learned relationships within sequences of sensory stimuli.

Networks of the dorsal-horn of the spinal-cord process nociceptive information from the periphery. In these networks, the excitation/inhibition balance is critical to shape this nociceptive information and to gate it to the brain where it is interpreted as pain. Our aim was to define whether short-term plasticity of inhibitory connections could tune this inhibition/excitation balance by differentially controlling excitatory and inhibitory microcircuits. To this end, we used spinal-cord slices from adult mice expressing enhanced green fluorescent protein (eGFP) under the GAD65 promoter and recorded from both eGFP+ (putative inhibitory) and eGFP- (putative excitatory) neurons of lamina II while stimulating single presynaptic GABAergic interneurons at various frequencies. Our results indicate that GABAergic neurons of lamina II simultaneously contact eGFP- and eGFP+ neurons, but these connections display very different frequency-dependent short-term plasticities. Connections onto eGFP- interneurons displayed limited frequency-dependent changes, and strong time-dependent summation of inhibitory synaptic currents that was however subjected to a tonic activity-dependent inhibition involving A1 adenosine receptors. In contrast, GABAergic connections onto eGFP+ interneurons expressed pronounced frequency-dependent depression, thus favoring disinhibition at these synapses by a mechanism involving the activation of GABAB autoreceptors at low frequency. Interestingly, the balance favors inhibition at frequencies associated with intense pain whether it favors excitation at frequencies associated with low pain. Therefore, these target- and frequency-specific plasticities allow to tune the balance between inhibition and disinhibition while processing frequency-coded information from primary afferents. These short-term plasticities and their modulation by A1 and GABAB receptors might represent an interesting target in pain-alleviating strategies.