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Pezizales are a diverse and economically important order of fungi. They are common in the environment, having epigeous form, such as morels and hypogeous, forms called truffles. The mature ascospores of most epigeous Pezizales are forcibly discharged through an opening at the ascus apex created with the lifting of the operculum, a lid-like structure specific to Pezizales. The axenic cultures of Pezizales fungi isolated from single ascospores are important for understanding the life cycle, development, ecology, and evolution of these fungi. However, obtaining single-spore isolates can be challenging, particularly for collections obtained in locations where sterile work environments are not available. In this paper, we introduce an accessible method for harvesting ascospores from fresh ascomata in the field and laboratory for obtaining single-spore isolates. Ascospores are harvested on the inside cover of Petri plate lids in the field, air dried, and stored. At a later date, single-spore isolates are axenically cultured through serial dilution and plating on antibiotic media. With this approach, we were able to harvest ascospores and obtain single-spore isolates from 12 saprotrophic and 2 ectomycorrhizal species belonging to six Pezizales families: Discinaceae, Morchellaceae, Pezizaceae, Pyronemataceae, Sarcosomataceae, and Sarcoscyphaceae. This method worked well for saprotrophic taxa (12 out of 19 species, 63%) and was even effective for a few ectomycorrhizal taxa (2 out of 13 species, 15%). This process was used to study the initial stages of spore germination and colony development in species across several Pezizales families. We found germination often commenced with the swelling of the spore, followed by the emergence of 1–8 germ tubes. This method is sufficiently straightforward that, provided with sterile Petri dishes, citizen scientists from distant locations could use this approach to capture spores and subsequently mail them with voucher specimens to a research laboratory for further study. The generated single-spore Pezizales isolates obtained through this method were used to generate high-quality genomic data. Isolates generated in this fashion can be used in manipulative experiments to better understand the biology, evolution, and ecogenomics of Pezizales.

This document is intended to introduce the general public to the chanterelle mushrooms of Florida and includes a preliminary key to Florida’s most common chanterelle species. Written by Kaori Hall, Ben Lemmond, and Matthew E. Smith, and published by the UF/IFAS Plant Pathology Department, February 2023.

Abstract Background In March 2016, citizens of Merrimack, New Hampshire, learned that their public water supply was contaminated with perfluorooctanoic acid (PFOA). A subsequent state-led investigation revealed widespread contamination of both public and private well water with PFOA and several related chemicals, broadly termed per- and polyfluoroalkyl substances (PFAS). This research examines the local response to PFAS contamination of the public water system and well water in Merrimack and the results from the health survey administered by a local advocacy group, Merrimack Citizens for Clean Water (MCFCW). Methods MCFCW designed and implemented a community health survey (n = 596) representing 213 households exposed to PFAS through drinking water. The surveys were conducted in the summer of 2017. Respondents used an online survey platform to report demographic information, exposure sources, and health conditions. Logistic regression was used to analyze the community-based health survey results . Results There were several important associations that warrant further investigation and more immediate attention, especially: 1) elevated incidence of developmental, autoimmune and kidney disorders among those under 18 years of age; 2) elevated levels of health concerns, multiple health concerns, autoimmune disorders, and reproductive disorders among women, 3) elevated levels of health concerns, multiple health conditions, cardiovascular, respiratory, reproductive, and liver disorders in those with industrial occupational exposures, and; 4) elevated incidence of health concerns, cardiovascular, and developmental disorders among those who have been living in Merrimack for a long time versus newer residents. Conclusions The limitations inherent in the study design warrant caution in interpreting the results, however the associations found in this study merit further investigation. This health survey highlights foremost the critical gap in information—lack of access to blood testing, medical monitoring and physician guidance of PFAS-exposed residents. This study provides a model for conducting community-based health studies to advocate for pathways to state supported biomonitoring and medical monitoring for those exposed to industrial toxins and to take into consideration the human health burden in shaping the future of chemical regulation.

Climate change driven food insecurity has emerged as a topic of special concern in the Canadian Arctic. Inuit communities in this region rely heavily on subsistence; however, access to traditional food sources may have been compromised due to climate change. Drawing from a total of 25 interviews among Inuit elders and experienced hunters from Cambridge Bay and Kugluktuk in Nunavut, Canada, this research examines how climate change is impacting food sovereignty and health. Our results show that reports of food insecurity were more pronounced in Kugluktuk than Cambridge Bay. Participants in Kugluktuk consistently noted declining availability of preferred fish and game species (e.g., caribou, Arctic char), a decline in participation of sharing networks, and overall increased difficulty accessing traditional foods. Respondents in both communities presented a consistent picture of climate change compounding existing socio-economic (e.g., poverty, disconnect between elders and youth) and health stressors affecting multiple aspects of food sovereignty. This article presents a situated understanding of how climate change as well as other sociocultural factors are eroding food sovereignty at the community-scale in the Arctic. We argue that a communal focus is required to address resilience and adaptation at the local level through programs that protect the local cultural knowledge, traditional ways of life, and indigenous sovereignty to reduce the severities of food insecurity in the Arctic stemming from climate change.