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The red imported fire ant, Solenopsis invicta Buren (Hymenoptera: Formicidae), originally is from South America and currently infests over 128 million ha in the US. Its presence has caused significant social, environmental, and economic impacts. Over the decades, chemical insecticides have controlled these pest ants successfully. However, this method is costly and unsustainable because red imported fire ant re-establishes colonies quickly after chemical application. Thus, it is important to develop additional strategies for managing the red imported fire ant in the US. Solenopsis invicta virus 3 is a positive-sense, single-stranded RNA virus specific for S. invicta that offers promise as a classical biological control agent or biopesticide for control of S. invicta. Surveys were conducted to determine the prevalence of Solenopsis invicta virus 3 in alates of S. invicta collected from 5 urban areas (Tallahassee, Pensacola, Jacksonville, Gainesville, and Panama City) and 5 adjacent rural areas (Quincy, Jay, Macclenny, Lake City, and Blountstown) of North Florida, USA, using the reverse transcription polymerase chain reaction technique. The prevalence of Solenopsis invicta virus 3 varied widely from city to city. No statistically significant differences in alate infection rate was found between urban and rural cities sampled. Areas in which no infections of Solenopsis invicta virus 3 were detected may be good candidates for the introduction of this virus as a biological control agent to help manage this pest locally. Resumen La hormiga roja de fuego importada, Solenopsis invicta Buren (Hymenoptera: Formicidae), es de origen Sudamericano y actualmente infesta mas de 128 millones de hectareas en los Estados Unidos. Su presencia ha provocado importantes impactos sociales, ambientales y economicos. Durante decadas, los insecticidas quimicos han controlado estas plagas de hormigas con exito. Sin embargo, este metodo es costoso e insostenible porque la hormiga roja de fuego importada restablece las colonias rapidamente despues de la aplicacion de productos quimicos. Por lo tanto, es importante desarrollar estrategias adicionales para el manejo de la hormiga roja de fuego importada en los EE. UU. Solenopsis invicta 3 es un virus de ARN monocatenario de sentido positivo especifico para S. invicta que es prometedor como agente de control biologico clasico o biopesticida para el control de S. invicta. Se realizo un sondeo para determinar la prevalencia del virus Solenopsis invicta 3 en individuos alados de S. invicta recolectados de 5 areas urbanas (Tallahassee, Pensacola, Jacksonville, Gainesville, y Ciudad de Panama) y 5 areas rurales adyacentes (Quincy, Jay, Macclenny, Lake City, y Blountstown) del norte de Florida, EE. UU., utilizando la tecnica de reaccion en cadena de la polimerasa con transcripcion inversa. La prevalencia del virus Solenopsis invicta 3 vario ampliamente de una ciudad a otra. No se encontraron diferencias estadisticamente significativas en la tasa de infeccion de los individuos alados entre las ciudades rurales y urbanas muestreadas. Las areas en las que no se detectaron infecciones por el virus Solenopsis invicta 3 pueden ser buenas candidatas para la introduccion de este virus como agente de control biologico para ayudar a manejar esta plaga localmente. Key Words: red imported fire ants; biological control; Solenopsis invicta virus 3 View this article in BioOne

Little evidence has been presented on the usefulness of sticky traps for monitoring bed bugs, Cimex lectularius. We examined how the surface roughness around the adhesive of a sticky trap affects both bed bug behavior and adhesive entrapment. In the first assay, bed bugs were placed onto acetate paper discs with different roughness averages (Ra). Each disc was surrounded by sticky trap adhesive and number of captured bed bugs were recorded. The second assay was set up similarly to the first assay except that the outer portion of the acetate disc had a different Ra than the center. In the third assay, bed bugs were placed into circular acetate arenas where they were surrounded by different Ra treatments. The number of times the bed bugs contacted the Ra treatment but did not cross onto the treatment was recorded. Results of these assays showed that as the acetate surfaces got smoother (lower Ra), bed bugs were more likely to get trapped in sticky trap adhesives but also less likely to travel across the smoother surfaces they encountered. A sticky trap design with a smooth plastic film around the adhesive was tested in the field to see if it could capture bed bugs in apartments with known bed bug activity. This trap was not only able to capture bed bugs but was also able to detect unknown German cockroach, Blattela germanica, infestations. Sticky trap designs with smooth surfaces around an adhesive could be used to monitor not only bed bugs but also German cockroaches.

Color and volatile stimulus preferences of Drosophila repleta (Patterson) Diptera: Drosophilidae), a nuisance pest of swine and poultry facilities, were tested using sticky card and bottle traps. Attractions to red, yellow, blue, orange, green, purple, black, grey and a white-on-black contrast treatment were tested in the laboratory. Drosophila repleta preferred red over yellow and white but not over blue. Other than showing preferences over the white control, D. repleta was not observed to have preferences between other colors and shade combinations. Pinot Noir red wine, apple cider vinegar, and wet swine feed were used in volatile preference field trials. Red wine was more attractive to D. repleta than the other volatiles tested, but there were no differences in response to combinations of a red wine volatile lure and various colors. Odor was found to play the primary role in attracting D. repleta.

The bed bug, Cimex lectularius, has re-established itself as a ubiquitous human ectoparasite throughout much of the world during the past two decades. This global resurgence is likely linked to increased international travel and commerce in addition to widespread insecticide resistance. Analyses of the C. lectularius sequenced genome (650 Mb) and 14,220 predicted protein-coding genes provide a comprehensive representation of genes that are linked to traumatic insemination, a reduced chemosensory repertoire of genes related to obligate hematophagy, host–symbiont interactions, and several mechanisms of insecticide resistance. In addition, we document the presence of multiple putative lateral gene transfer events. Genome sequencing and annotation establish a solid foundation for future research on mechanisms of insecticide resistance, human–bed bug and symbiont–bed bug associations, and unique features of bed bug biology that contribute to the unprecedented success of C. lectularius as a human ectoparasite., The bed bug, Cimex lectularius, is a ubiquitous human ectoparasite with global distribution. Here, the authors sequence the genome of the bed bug and identify reductions in chemosensory genes, expansion of genes associated with blood digestion and genes linked to pesticide resistance.

Little is known about what factors influence the climbing ability of bed bugs, Cimex lectularius L. (Hemiptera: Cimicidae), in relation to the various surfaces they encounter. We examined how sex, time since last fed, and what surfaces the bed bugs were in contact with affected their climbing performance. The effects of sex and time since fed were tested by counting the number of bed bugs able to climb a 45° slope. The pulling force was recorded using an analytical balance technique that captured the sequential vertical pulling force output of bed bugs attached to various surfaces. Recently fed female bed bugs were found to have the most difficulty in climbing smooth surfaces in comparison with males. This difference can be explained by the larger weight gained from bloodmeals by female bed bugs. A variety of vertical pulling forces were observed on surfaces ranging from sandpaper to talc powder-covered glass. For surfaces not treated with talc powder, bed bugs generated the least amount of vertical pulling force from synthetically created 0.6-µm plastron surfaces. This vast range in the ability of bed bugs to grip onto various surfaces may have implications on limiting bed bugs dispersal and hitchhiking behaviors.