1. Lower expression of platelet derived growth factor is associated with better overall survival rate of patients with idiopathic nonspecific interstitial pneumonia
- Author
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Ziling Huang, Benfang Luo, Weizhe Qiu, Fei Han, Xia Fang, Xianghua Yi, Pan Gu, Wei Chen, Long Zhang, Xuyou Zhu, Weiwei Rui, and Yu Zeng
- Subjects
0301 basic medicine ,Pulmonary and Respiratory Medicine ,FGF10 ,Platelet-derived growth factor ,biology ,business.industry ,medicine.disease ,Pathogenesis ,03 medical and health sciences ,Idiopathic pulmonary fibrosis ,chemistry.chemical_compound ,030104 developmental biology ,0302 clinical medicine ,030228 respiratory system ,chemistry ,Fibrosis ,Immunology ,biology.protein ,Immunohistochemistry ,Medicine ,Original Article ,business ,Survival rate ,Platelet-derived growth factor receptor - Abstract
Background: Idiopathic nonspecific interstitial pneumonia (INSIP) presents with varying degrees of interstitial inflammation and fibrosis exhibiting a uniform appearance. Lack of knowledge on the underlying mechanisms of INSIP has contributed to few effective treatment strategies. Our study is designed to explore aberrantly expressed cytokines involvement in INSIP development. Methods: Oligo GEArray was employed to detect the expression of cytokines in INSIP patients, and idiopathic pulmonary fibrosis (IPF) was setup as isotype control. Real-time PCR and immunohistochemistry analysis were used to further confirm the expression of abnormally expressed cytokines. The correlationship between cytokines expression and overall survival rate of patients with IPF and INSIP were analyzed. Results: From microarray detection, transforming growth factor-beta-1 (TGF-β1), fibroblast growth factor 10 (FGF10), and platelet derived growth factor (PDGF) were predominantly up-regulated in patients with INSIP. Real-time PCR and immunohistochemistry also showed these cytokines was abnormally expressed in INSIP. In addition to, the clinical relevance analysis demonstrated relatively lower expression of PDGF patients had longer overall survival rate than those with higher expression of PDGF. Conclusions: Our study suggests that TGF-β1, FGF10, and PDGF are required for the pathogenesis of INSIP, and may therefore be ideal targets in INSIP treatment. Moreover, INSIP patients with lower expression of PDGF had better survival rate.
- Published
- 2017