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Your search keyword '"Benestad, Sylvie L. [0000-0002-3011-0484]"' showing total 4 results
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4 results on '"Benestad, Sylvie L. [0000-0002-3011-0484]"'

1. Classical BSE dismissed as the cause of CWD in Norwegian red deer despite strain similarities between both prion agents

Author

Conferencia de Rectores de las Universidades Españolas, Consejo Superior de Investigaciones Científicas (España), European Commission, Agencia Estatal de Investigación (España), Istituto Superiore di Sanità, Ministerio de Ciencia, Innovación y Universidades (España), Marín-Moreno, Alba [0000-0002-4023-6398], Benestad, Sylvie L. [0000-0002-3011-0484], Barrio, Tomás [0000-0002-7037-6316], Pirisinu, Laura [0000-0002-6548-8225], Espinosa, Juan Carlos [0000-0002-6719-9902], di Bari, Michele [0000-0003-0943-6823], Eraña, Hasier [0000-0001-8776-4211], Maddison, Ben C. [0000-0001-5453-5359], Canoyra, Sara [0000-0001-6371-8122], Castilla, Joaquín [0000-0002-2216-1361], Spiropoulos, J. [0000-0003-0119-8920], Bishop, Keith [0009-0009-8486-9486], Nonno, Romolo [0000-0001-7556-1564], Våge, Jorn [0000-0002-2016-0111], Andréoletti, Olivier [0000-0002-7369-6016], Torres, Juan María [0000-0003-0443-9232], Marín-Moreno, Alba, Benestad, Sylvie L., Barrio, Tomás, Pirisinu, Laura, Espinosa, Juan Carlos, Tran, Linh, Huor, Alvina, di Bari, Michele, Eraña, Hasier, Maddison, Ben C., D'Agostino, Claudia, Fernández-Borges, N., Canoyra, Sara, Jerez-Garrido, Nuria, Castilla, Joaquín, Spiropoulos, J., Bishop, Keith, Gough, Kevin C., Nonno, Romolo, Våge, Jorn, Andréoletti, Olivier, Torres, Juan María, Conferencia de Rectores de las Universidades Españolas, Consejo Superior de Investigaciones Científicas (España), European Commission, Agencia Estatal de Investigación (España), Istituto Superiore di Sanità, Ministerio de Ciencia, Innovación y Universidades (España), Marín-Moreno, Alba [0000-0002-4023-6398], Benestad, Sylvie L. [0000-0002-3011-0484], Barrio, Tomás [0000-0002-7037-6316], Pirisinu, Laura [0000-0002-6548-8225], Espinosa, Juan Carlos [0000-0002-6719-9902], di Bari, Michele [0000-0003-0943-6823], Eraña, Hasier [0000-0001-8776-4211], Maddison, Ben C. [0000-0001-5453-5359], Canoyra, Sara [0000-0001-6371-8122], Castilla, Joaquín [0000-0002-2216-1361], Spiropoulos, J. [0000-0003-0119-8920], Bishop, Keith [0009-0009-8486-9486], Nonno, Romolo [0000-0001-7556-1564], Våge, Jorn [0000-0002-2016-0111], Andréoletti, Olivier [0000-0002-7369-6016], Torres, Juan María [0000-0003-0443-9232], Marín-Moreno, Alba, Benestad, Sylvie L., Barrio, Tomás, Pirisinu, Laura, Espinosa, Juan Carlos, Tran, Linh, Huor, Alvina, di Bari, Michele, Eraña, Hasier, Maddison, Ben C., D'Agostino, Claudia, Fernández-Borges, N., Canoyra, Sara, Jerez-Garrido, Nuria, Castilla, Joaquín, Spiropoulos, J., Bishop, Keith, Gough, Kevin C., Nonno, Romolo, Våge, Jorn, Andréoletti, Olivier, and Torres, Juan María

Abstract

The first case of CWD in a Norwegian red deer was detected by a routine ELISA test and confirmed by western blotting and immunohistochemistry in the brain stem of the animal. Two different western blotting tests were conducted independently in two different laboratories, showing that the red deer glycoprofile was different from the Norwegian CWD reindeer and CWD moose and from North American CWD. The isolate showed nevertheless features similar to the classical BSE (BSE-C) strain. Furthermore, BSE-C could not be excluded based on the PrPSc immunohistochemistry staining in the brainstem and the absence of detectable PrPSc in the lymphoid tissues. Because of the known ability of BSE-C to cross species barriers as well as its zoonotic potential, the CWD red deer isolate was submitted to the EURL Strain Typing Expert Group (STEG) as a BSE-C suspect for further investigation. In addition, different strain typing in vivo and in vitro strategies aiming at identifying the BSE-C strain in the red deer isolate were performed independently in three research groups and BSE-C was not found in it. These results suggest that the Norwegian CWD red deer case was infected with a previously unknown CWD type and further investigation is needed to determine the characteristics of this potential new CWD strain.

Published
2024

2. Porcine Prion Protein as a Paradigm of Limited Susceptibility to Prion Strain Propagation

Author

Ministerio de Economía y Competitividad (España), Agencia Estatal de Investigación (España), European Commission, Food Standards Agency (UK), Espinosa Martín, Juan Carlos [0000-0002-6719-9902], Marín-Moreno, Alba (0000-0002-4023-6398), Benestad, Sylvie L (0000-0002-3011-0484), Andreoletti, Olivier (0000-0002-7369-6016), Torres, Juan María (0000-0003-0443-9232), Espinosa Martín, Juan Carlos, Marín-Moreno, Alba, Aguilar-Calvo, Patricia, Benestad, Sylvie L., Andréoletti, Olivier, Torres, Juan María, Ministerio de Economía y Competitividad (España), Agencia Estatal de Investigación (España), European Commission, Food Standards Agency (UK), Espinosa Martín, Juan Carlos [0000-0002-6719-9902], Marín-Moreno, Alba (0000-0002-4023-6398), Benestad, Sylvie L (0000-0002-3011-0484), Andreoletti, Olivier (0000-0002-7369-6016), Torres, Juan María (0000-0003-0443-9232), Espinosa Martín, Juan Carlos, Marín-Moreno, Alba, Aguilar-Calvo, Patricia, Benestad, Sylvie L., Andréoletti, Olivier, and Torres, Juan María

Abstract

Although experimental transmission of bovine spongiform encephalopathy (BSE) to pigs and transgenic mice expressing pig cellular prion protein (PrPC) (porcine PrP [PoPrP]-Tg001) has been described, no natural cases of prion diseases in pig were reported. This study analyzed pig-PrPC susceptibility to different prion strains using PoPrP-Tg001 mice either as animal bioassay or as substrate for protein misfolding cyclic amplification (PMCA). A panel of isolates representatives of different prion strains was selected, including classic and atypical/Nor98 scrapie, atypical-BSE, rodent scrapie, human Creutzfeldt-Jakob-disease and classic BSE from different species. Bioassay proved that PoPrP-Tg001-mice were susceptible only to the classic BSE agent, and PMCA results indicate that only classic BSE can convert pig-PrPC into scrapie-type PrP (PrPSc), independently of the species origin. Therefore, conformational flexibility constraints associated with pig-PrP would limit the number of permissible PrPSc conformations compatible with pig-PrPC, thus suggesting that pig-PrPC may constitute a paradigm of low conformational flexibility that could confer high resistance to the diversity of prion strains.

Published
2021

3. Erratum to: Porcine Prion Protein as a Paradigm of Limited Susceptibility to Prion Strain Propagation

Author

Espinosa Martín, Juan Carlos [0000-0002-6719-9902], Marín-Moreno, Alba (0000-0002-4023-6398), Benestad, Sylvie L (0000-0002-3011-0484), Andreoletti, Olivier (0000-0002-7369-6016), Torres, Juan María (0000-0003-0443-9232), Espinosa Martín, Juan Carlos, Marín-Moreno, Alba, Aguilar-Calvo, Patricia, Benestad, Sylvie L., Andréoletti, Olivier, Torres, Juan María, Espinosa Martín, Juan Carlos [0000-0002-6719-9902], Marín-Moreno, Alba (0000-0002-4023-6398), Benestad, Sylvie L (0000-0002-3011-0484), Andreoletti, Olivier (0000-0002-7369-6016), Torres, Juan María (0000-0003-0443-9232), Espinosa Martín, Juan Carlos, Marín-Moreno, Alba, Aguilar-Calvo, Patricia, Benestad, Sylvie L., Andréoletti, Olivier, and Torres, Juan María

Abstract

In "Porcine Prion Protein as a Paradigm of Limited Susceptibility to Prion Strain Propagation" by Espinosa et al. [J Infect Dis, doi:10.1093/infdis/jiz646], there was an error in Figure 2 and the footnote corresponding to Supplementary Figure 1 was missing. These have been corrected in the original article. (Figure Presented)., This is a correction to: The Journal of Infectious Diseases, Volume 223, Issue 6, 15 March 2021, Pages 1103–1112, https://doi.org/10.1093/infdis/jiz646

Published
2020

4. Porcine Prion Protein as a Paradigm of Limited Susceptibility to Prion Strain Propagation

Author

Juan Carlos Espinosa, Juan María Torres, Olivier Andreoletti, Patricia Aguilar-Calvo, Alba Marín-Moreno, Sylvie L. Benestad, Centro de Investigacion en Sanidad Animal (INIA-CISA), Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria = National Institute for Agricultural and Food Research and Technology (INIA), Norwegian Veterinary Institute [Oslo], Interactions hôtes-agents pathogènes [Toulouse] (IHAP), Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), This work was supported by the Spanish Ministerio de Economía y Competitividad (grants AGL2012-37988-C04-04 and AGL2016-78054-R [Agencia Estatal de Investigación/Fondo Europeo de Desarrollo Regional, Unión Europea] to J. C. E. and J. M. T., fellowship BES-2010–040922 to P. A. C., and fellowship INIA-FPI-SGIT-2015-02 to A. M. M.), the UK Food Standards Agency (project FS231051 ['Permeability of the Human Species Barriers to TSE Circulating Agents']), and the Fonds Europeens de Developpement Regional Programme Operationnel de Cooperation Territoriale Espagne France Andorre REDPRION (project EFA148/16 [REDPRION] to O. A.)., Ministerio de Economía y Competitividad (España), Agencia Estatal de Investigación (España), European Commission, Food Standards Agency (UK), Espinosa, Juan Carlos, Marín-Moreno, Alba (0000-0002-4023-6398), Benestad, Sylvie L (0000-0002-3011-0484), Andreoletti, Olivier (0000-0002-7369-6016), and Torres, Juan María (0000-0003-0443-9232)

Subjects
0301 basic medicine, Genetically modified mouse, pig, Bovine spongiform encephalopathy, animal diseases, [SDV]Life Sciences [q-bio], Prion strain, Scrapie, Biology, BSE, 03 medical and health sciences, 0302 clinical medicine, mental disorders, medicine, Immunology and Allergy, Bioassay, Prion protein, classic scrapie, PrP, prion conversion, atypical/Nor98 scrapie, prion strains, swine, medicine.disease, Virology, nervous system diseases, High resistance, 030104 developmental biology, Infectious Diseases, Protein Misfolding Cyclic Amplification, 030217 neurology & neurosurgery
Abstract

10 Pág. Centro de Investigación en Sanidad Animal (CISA), Although experimental transmission of bovine spongiform encephalopathy (BSE) to pigs and transgenic mice expressing pig cellular prion protein (PrPC) (porcine PrP [PoPrP]-Tg001) has been described, no natural cases of prion diseases in pig were reported. This study analyzed pig-PrPC susceptibility to different prion strains using PoPrP-Tg001 mice either as animal bioassay or as substrate for protein misfolding cyclic amplification (PMCA). A panel of isolates representatives of different prion strains was selected, including classic and atypical/Nor98 scrapie, atypical-BSE, rodent scrapie, human Creutzfeldt-Jakob-disease and classic BSE from different species. Bioassay proved that PoPrP-Tg001-mice were susceptible only to the classic BSE agent, and PMCA results indicate that only classic BSE can convert pig-PrPC into scrapie-type PrP (PrPSc), independently of the species origin. Therefore, conformational flexibility constraints associated with pig-PrP would limit the number of permissible PrPSc conformations compatible with pig-PrPC, thus suggesting that pig-PrPC may constitute a paradigm of low conformational flexibility that could confer high resistance to the diversity of prion strains., This work was supported by the Spanish Ministerio de Economía y Competitividad (grants AGL2012- 37988-C04-04 and AGL2016-78054-R [Agencia Estatal de Investigación/Fondo Europeo de Desarrollo Regional, Unión Europea] to J. C. E. and J. M. T., fellowship BES-2010–040922 to P. A. C., and fellowship INIA-FPI-SGIT-2015-02 to A. M. M.), the UK Food Standards Agency (project FS231051 [“Permeability of the Human Species Barriers to TSE Circulating Agents”]), and the Fonds Europeens de Developpement Regional Programme Operationnel de Cooperation Territoriale Espagne France Andorre REDPRION (project EFA148/16 [REDPRION] to O. A.)

Published
2021

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