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1. Assessment Tools and Rehabilitation Treatments for Aprosodia Following Acquired Brain Injury: A Scoping Review

Author

Benedetti, Valentina, Weill-Chounlamountry, Agnès, Pradat-Diehl, Pascale, and Villain, Marie

Abstract

Background: Acquired brain injury (ABI), especially to the right hemisphere, can result in difficulty using or understanding prosodic contours in speech. Prosody is used to convey emotional connotation or linguistic intent and includes pitch, loudness, rate, and voice quality. A disorder in the comprehension or production of prosody is known as aprosodia; despite the communication disability caused by prosodic disorders, the assessment and treatment of aprosodia following ABI has received scant attention. Aims: The aim of this scoping review is to gather and synthesise useful knowledge on aprosodia and provide therapists with an exhaustive document in order to guide clinical decision-making encouraging active identification and treatment of this disorder. Methods & Procedures: This scoping review, conducted in accordance with PRISMA-ScR guidelines, investigated the existing literature concerning the assessment and treatments of linguistic and affective aprosodia in adult patients after ABI. A systematic search in four electronic databases (PubMed, CINAHL, Web of Science, ScienceDirect) was conducted for articles written in English, French, or Italian published between 1970 and 2020. After all evaluative criteria were applied, 15 articles were included for final review. Outcomes & Results: Results show the presence of six assessment tools for affective aprosodia and five evaluation tools targeting affective and linguistic prosody. Assessment of aprosodia is generally accomplished through acoustic and perceptual approaches. Current treatments for prosodic disorders focus on expressive aprosodia and have applied mostly two different approaches: imitative and cognitive-linguistic methods. Conclusion & Implications: Findings suggest that aprosodia can be assessed by therapists through various techniques and may be amenable to behavioural treatments. Nevertheless, although there are several assessment tools available, no one currently offers a comprehensive assessment that incorporates an ecological dimension. It therefore seems necessary to continue research in this direction. The rehabilitation of receptive prosody abilities also remains to be explored.

Published
2022
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2. Convalescent or standard plasma versus standard of care in the treatment of COVID-19 patients with respiratory impairment: short and long-term effects. A three-arm randomized controlled clinical trial

Author

Manzini, Paola Maria, Ciccone, Giovannino, De Rosa, Francesco Giuseppe, Cavallo, Rossana, Ghisetti, Valeria, D’Antico, Sergio, Galassi, Claudia, Saccona, Fabio, Castiglione, Anna, Birocco, Nadia, Francisci, Tiziana, Hu, Huijing, Pecoraro, Clara, Danielle, Franca, Labanca, Luciana, Bordiga, Anna Maria, Lorenzi, Marco, Camisasca, Giovanni, Giachino, Osvaldo, Pagliarino, Mauro, Ottone, Piero, Scuvera, Ilvana Tiziana Donatella, Guaschino, Roberto, Freilone, Roberto, Berti, Pierluigi, Pittaluga, Fabrizia, Avolio, Maria, Costa, Cristina, Raso, Samuele, Nucci, Aurora, Milan, Massimo, Baffa, Alessandra, Russo, Alessandra, Tornello, Antonella, Maddalena, Laura, Delios, Grazia, Marletto, Fabio Paolo, De Micheli, Anna Grazia, Mattei, Alessio, Baldassano, Stefano, Canta, Francesca, Russo, Maria Luisa, Bergamo, Daniele, Vitale, Francesco, Liccardi, Marco Maria, Chinaglia, Alessandra, Calcagno, Andrea, Converso, Marcella, Aldieri, Chiara, Libanore, Valentina, Blangetti, Ilaria, Benedetti, Valentina, Mitola, Barbara, and Scozzari, Gitana

Published
2022
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8. List of Contributors

Author

Abecassis, Michael, primary, Abed, Ahmed, additional, Abelli, Massimo, additional, Ahmad, Sarwat, additional, Alessiani, Mario, additional, Alsharidi, Aynaa, additional, Altruda, Fiorella, additional, Amorese, Gabriella, additional, Angeletti, Andrea, additional, Angelico, Mario, additional, Angelico, Roberta, additional, Angelotti, Maria L., additional, Applegate, Robert J., additional, Atala, Anthony, additional, Attanasio, Chiara, additional, Axelrod, David, additional, Bababekov, Yanik, additional, Basu, Joydeep, additional, Becherucci, Francesca, additional, Belford, P. Matthew, additional, Benedetti, Enrico, additional, Benedetti, Valentina, additional, Benigni, Ariela, additional, Bertram, Timothy A., additional, Bestard, Oriol, additional, Blake, Joshua, additional, Boggi, Ugo, additional, Brasile, Lauren, additional, Bromberg, Jonathan S., additional, Brouard, Sophie, additional, Brovold, Matthew, additional, Burke, George W., additional, Busic, Mirela, additional, Butt, Zeeshan, additional, Caddeo, Silvia, additional, Calzone, Stefano, additional, Campistol, Josep M., additional, Carmagnola, Irene, additional, Carty, Fiona, additional, Castanares-Zapatero, Diego, additional, Chadjichristos, Christos E., additional, Chambers, Brooke E., additional, Chandran, Sindhu, additional, Chatziantoniou, Christos, additional, Chen, Ashton, additional, Chen, Linda, additional, Chen, Xiwu, additional, Chiono, Valeria, additional, Chiusa, Manuel, additional, Ciancio, Gaetano, additional, Ciardelli, Gianluca, additional, Coletti, Gino, additional, Coll, Elisabeth, additional, Collienne, Christine, additional, Colvin, Robert B., additional, Cortinovis, Monica, additional, Cosimi, A. Benedict, additional, Cravedi, Paolo, additional, D’Amico, Giuseppe, additional, Da Sacco, Stefano, additional, Danger, Richard, additional, Dantal, Jacques, additional, Davidson, Alan J., additional, De Chiara, Letizia, additional, De Fijter, Johannes W., additional, de la Rosa, Gloria, additional, Delmonico, Francis L., additional, Deng, Junhong, additional, Deurdulian, Corinne, additional, Dhal, Abritee, additional, Dionigi, Paolo, additional, Domínguez-Gil, Beatriz, additional, Drozdzik, Marek, additional, Dussaule, Jean-Claude, additional, Edgar, Lauren, additional, Egidi, Maria Francesca, additional, El Hennawy, Hany, additional, English, Karen, additional, Everly, Matthew J., additional, Fagoonee, Sharmila, additional, Famulari, Elvira Smeralda, additional, Farney, Alan C., additional, Figliuzzi, Marina, additional, Framarino-dei-Malatesta, Marialuisa, additional, Fumo, David E., additional, Gagliardini, Elena, additional, Gallon, Lorenzo, additional, Gandhi, Sanjay K., additional, Gautreaux, Michael D., additional, Geraedts, Anna, additional, Giannese, Domenico, additional, Giulianotti, Pier C., additional, Goligorsky, Michael S., additional, Griesemar, Adam, additional, Grinyó, Josep M., additional, Gruessner, Angelika C., additional, Gruessner, Rainer W.G., additional, He, Bulang, additional, Heher, Eliot, additional, Ho, Bing, additional, Hosgood, Sarah A., additional, Hotta, Kiyohiko, additional, Humar, Atul, additional, Humes, H. David, additional, Iaria, Giuseppe, additional, Imberti, Barbara, additional, Izpisua Belmonte, Juan Carlos, additional, Jochmans, Ina, additional, Katari, Ravi, additional, Kavvadas, Panagiotis, additional, Kawai, Tatsuo, additional, Kengla, Carlos, additional, Keys, Tristan, additional, Kidiyoor, Amritha, additional, Kidokoro, Kengo, additional, Kumar, Deepali, additional, Kutcher, Michael A., additional, Lai, Quirino, additional, Laterre, Pierre-François, additional, Lebbé, Céleste, additional, Legendre, Christophe, additional, Lennon, Rachel, additional, Li, Peng, additional, Lin, Jen-Jar, additional, Little, Melissa H., additional, Lu, Xiongbing, additional, Ludlow, John W., additional, Mahíllo, Beatriz, additional, Masereeuw, Rosalinde, additional, Matesanz, Rafael, additional, Matovinovic, Mirjana S., additional, Mazzinghi, Benedetta, additional, Mbiandjeu Toya, Serge Cedrick, additional, McEwen, Scott, additional, Melandro, Fabio, additional, Melchiorri, Loredana, additional, Menon, Madhav C., additional, Mirzazadeh, Majid, additional, Montag, Samantha, additional, Montgomery, Robert A., additional, Montiel, Virginie, additional, Montserrat, Nuria, additional, Morigi, Marina, additional, Morrill, Christian C., additional, Mourad, Michel, additional, Murphy, Sean V., additional, Murray, Patricia, additional, Nardo, Tiziana, additional, Netti, Paolo A., additional, Nguyen, Mark, additional, Nicholson, Michael L., additional, O’Callaghan, John M., additional, Ohler, Linda, additional, Orlando, Giuseppe, additional, Osafune, Kenji, additional, Oura, Tetsu, additional, Peired, Anna, additional, Peloso, Andrea, additional, Peng, Zhenzhen, additional, Perico, Norberto, additional, Perin, Laura, additional, Perrone, Vittorio, additional, Persad, Paul, additional, Peti-Peterdi, János, additional, Petrosyan, Astgik, additional, Pietrabissa, Andrea, additional, Pino, Christopher J., additional, Pirenne, Jacques, additional, Pisani, Francesco, additional, Ploeg, Rutger J., additional, Porrini, Esteban, additional, Pozzi, Ambra, additional, Pugliese, Alberto, additional, Pugliese, Luigi, additional, Rabelink, Ton J., additional, Rampino, Teresa, additional, Rees, Michael A., additional, Reinders, Marlies E.J., additional, Remuzzi, Andrea, additional, Remuzzi, Giuseppe, additional, Riquier-Brison, Anne, additional, Roca, Raquel G., additional, Rogers, Jeffrey, additional, Romagnani, Paola, additional, Rosales, Ivy A., additional, Rosenblum, Norman D., additional, Rota, Cinzia, additional, Ruggenenti, Piero, additional, Ruini, Francesca, additional, Sageshima, Junichiro, additional, El Salem, Fadi, additional, Sandal, Shaifali, additional, Sander, Veronika, additional, Santeramo, Ilaria, additional, Santos, Renato M., additional, Sarwal, Minnie, additional, Shah, Jigesh, additional, Shetty, Aneesha A., additional, Silengo, Lorenzo, additional, Siskind, Eric, additional, Skaro, Anton, additional, Snanoudj, Renaud, additional, Soker, Shay, additional, South, Andrew M., additional, Spasovski, Goce, additional, Stratta, Robert J., additional, Strom, Charles, additional, Stubenitsky, Bart, additional, Tamburrini, Riccardo, additional, Tang, Qizhi, additional, Tantissattamo, Ekamol, additional, Tasaki, Masayuki, additional, Tchelepi, Hisham, additional, Ticozzelli, Elena, additional, Traitanon, Opas, additional, Trillini, Matias, additional, Tzvetanov, Ivo, additional, Valentín, María O., additional, Vincenti, Flavio, additional, Vistoli, Fabio, additional, Vriend, Jelle, additional, Walker, Stephen J., additional, Wertheim, Jason A., additional, Williams, David F., additional, Wilm, Bettina, additional, Wilmer, Martijn J., additional, Wingert, Rebecca A., additional, Wittebole, Xavier, additional, Xia, Yun, additional, Xinaris, Christodoulos, additional, Yamada, Kazuhiko, additional, Yokoo, Takashi, additional, Yokote, Shinya, additional, Zandvliet, Maarten L., additional, Zent, Roy, additional, Zhang, Yuanyuan, additional, Zhao, David X., additional, Zhao, Lihui, additional, and Zhao, Susan Y., additional

Published
2017
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12. Additional file 1 of Convalescent or standard plasma versus standard of care in the treatment of COVID-19 patients with respiratory impairment: short and long-term effects. A three-arm randomized controlled clinical trial

Author

Manzini, Paola Maria, Ciccone, Giovannino, De Rosa, Francesco Giuseppe, Cavallo, Rossana, Ghisetti, Valeria, D’Antico, Sergio, Galassi, Claudia, Saccona, Fabio, Castiglione, Anna, Birocco, Nadia, Francisci, Tiziana, Hu, Huijing, Pecoraro, Clara, Danielle, Franca, Labanca, Luciana, Bordiga, Anna Maria, Lorenzi, Marco, Camisasca, Giovanni, Giachino, Osvaldo, Pagliarino, Mauro, Ottone, Piero, Scuvera, Ilvana Tiziana Donatella, Guaschino, Roberto, Freilone, Roberto, Berti, Pierluigi, Pittaluga, Fabrizia, Avolio, Maria, Costa, Cristina, Raso, Samuele, Nucci, Aurora, Milan, Massimo, Baffa, Alessandra, Russo, Alessandra, Tornello, Antonella, Maddalena, Laura, Delios, Grazia, Marletto, Fabio Paolo, De Micheli, Anna Grazia, Mattei, Alessio, Baldassano, Stefano, Canta, Francesca, Russo, Maria Luisa, Bergamo, Daniele, Vitale, Francesco, Liccardi, Marco Maria, Chinaglia, Alessandra, Calcagno, Andrea, Converso, Marcella, Aldieri, Chiara, Libanore, Valentina, Blangetti, Ilaria, Benedetti, Valentina, Mitola, Barbara, and Scozzari, Gitana

Abstract

Additional file 1. Supplementary appendix.

Published
2022
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15. Predicting candidemia in Internal Medicine Units: are we chasing the Holy Grail?

Author

Pieralli, Filippo, primary, Azzini, Anna Maria, additional, Concia, Ercole, additional, Lo Cascio, Giuliana, additional, Tedesco, Andrea, additional, Benedetti, Valentina, additional, Piredda, Sabrina, additional, Giusti, Massimo, additional, Santini, Claudio, additional, Zagarrì, Elisa, additional, Fontanella, Andrea, additional, and Manfellotto, Dario, additional

Published
2021
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22. Los celos y el uso de las redes sociales en parejas universitarias de Cali

Author

Consultor de tesis, Valderrama, Laura, Rodríguez, María José, Benedetti, Valentina, Consultor de tesis, Valderrama, Laura, Rodríguez, María José, and Benedetti, Valentina

Abstract

La investigación tuvo como objetivo, identificar los niveles de celos en uno de los miembros de la pareja en estudiantes universitarios para posteriormente identificar la relación entre dicho nivel y las redes sociales, en una era en que la interconectividad y la tecnología juegan un papel protagónico. También se analizaron las diferencias que existen entre hombres y mujeres respecto a la forma en que manifiestan los celos. Para lo anterior se utilizó un método mixto en el que 80 participantes respondieron a la Escala Interpersonal de Celos adaptada por Rodríguez y Martínez (1996) y posteriormente se realizó un grupo focal con los puntajes más altos. Entre los principales resultados se encontró que el uso de las redes sociales tiene un alto impacto en el aumento de conductas celosas, comportamientos obsesivo compulsivos, violación a la privacidad, baja autoestima y aumento de ansiedad, todo concerniente a la relación de pareja., The research aimed to identify levels of jealousy in one of the members of the couple in university students to later identify the relationship between that level and social networks, in an era in which interconnectivity and technology play a leading role. The differences between men and women regarding the way they manifest jealousy were also analyzed. For the above, a mixed method was used in which 80 participants responded to the Interpersonal Jealousy Scale adapted by Rodríguez and Martínez (1996) and subsequently a focus group with the highest scores was made. Among the main results, it was found that the use of social networks has a high impact on the increase of jealous behaviors, obsessive compulsive behaviors, privacy violation, low self-esteem and increased anxiety, all concerning the couple's relationship.

Published
2019

24. Alteration of thyroid hormone signaling triggers the diabetes-induced pathological growth, remodeling, and dedifferentiation of podocytes

Author

Benedetti, Valentina, primary, Lavecchia, Angelo Michele, additional, Locatelli, Monica, additional, Brizi, Valerio, additional, Corna, Daniela, additional, Todeschini, Marta, additional, Novelli, Rubina, additional, Benigni, Ariela, additional, Zoja, Carlamaria, additional, Remuzzi, Giuseppe, additional, and Xinaris, Christodoulos, additional

Published
2019
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25. Study protocol: safety and efficacy of propranolol in newborns with Retinopathy of Prematurity (PROP-ROP): ISRCTN18523491

Author

Isacchi Benedetta, Furlanetto Sandra, Bilia Anna, Monte Massimo, Ristori Chiara, Bagnoli Paola, Malvagia Sabrina, Marca Giancarlo, Fortunato Pina, Pollazzi Liliana, Torre Agostino, Ramenghi Luca, Araimo Gabriella, Cristofori Gloria, Benedetti Valentina, Daniotti Marta, Fiorini Patrizio, Cavallaro Giacomo, Filippi Luca, Tinelli Francesca, Cioni Giovanni, Donzelli Gianpaolo, Osnaghi Silvia, and Mosca Fabio

Subjects
Pediatrics, RJ1-570
Abstract

Abstract Background Despite new therapeutic approaches have improved the prognosis of newborns with retinopathy of prematurity (ROP), an unfavourable structural and functional outcome still remains high. There is high pressure to develop new drugs to prevent and treat ROP. There is increasing enthusiasm for anti-VEGF drugs, but angiogenic inhibitors selective for abnormal blood vessels would be considered as an optimal treatment. In an animal experimental model of proliferative retinopathy, we have recently demonstrated that the pharmacological blockade of beta-adrenoreceptors improves retinal neovascularization and blood retinal barrier breakdown consequent to hypoxia. The purpose of this study is to evaluate the propranolol administration in preterm newborns suffering from a precocious phase of ROP in terms of safety and efficacy in counteracting the progression of retinopathy. Methods/Design Preterm newborns (gestational age at birth lower than 32 weeks) with stage 2 ROP (zone II-III without plus) will be randomized, according to their gestational age, to receive propranolol added to standard treatment (treatment adopted by the ETROP Cooperative Group) or standard treatment alone. Propranolol will be administered until retinal vascularization will be completely developed, but not more than 90 days. Forty-four participants will be recruited into the study. To evaluate the safety of propranolol administration, cardiac and respiratory parameters will be continuously monitored. Blood samplings will be performed to check renal, liver and metabolic balance. To evaluate the efficacy of propranolol, the progression of the disease, the number of laser treatments or vitrectomies, the incidence of retinal detachment or blindness, will be evaluated by serial ophthalmologic examinations. Visual function will be evaluated by means of behavioural standardized tests. Discussion This pilot study is the first research that explores the possible therapeutic role of beta blockers in ROP. The objective of this research is highly ambitious: to find a treatment simple, inexpensive, well tolerated and with few adverse effects, able to counteract one of the major complications of the prematurity. Any favourable results of this research could open new perspectives and original scenarios about the treatment or the prevention of this and other proliferative retinopathies. Trial Registration Current Controlled Trials ISRCTN18523491; ClinicalTrials.gov Identifier NCT01079715; EudraCT Number 2010-018737-21

Published
2010
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27. The other side of an eating disorder: a thematic analysis of recovery stories shared on Instagram

Author

Benedetti, Valentina and Benedetti, Valentina

Abstract

The photo and video sharing platform Instagram has been widely utilised for recovery purposes by individuals affected by an eating disorder. Since recovery is a critical yet poorly comprehended subject, the present qualitative study employed Thematic Analysis to examine the visual and textual content of 956 posts related to eating disorder recovery, published on Instagram by using the hashtags #eatingdisorderrecovery; #bulimarecovery; #anorexiarecovery; #edwarrior; #EDrecoveryfamily. The analysis of visual material generated six popular photo categories, while the assessment of captions revealed four broad themes: Separating the Self from the disorder, Shaping a New Identity, The Emotional side of recovery and Being part of a Family of Warriors. Despite the process mainly involves self-reflection and identity exploration, recovery on Instagram seems to be strongly subjected to stereotypical western views but also benefit from positive social interactions. Therapeutic measures could be integrated by online–based schemes to maximise treatment efficacy and reduce chances of future relapse.

Published
2018

31. Functional Human Podocytes Generated in Organoids from Amniotic Fluid Stem Cells

Author

Xinaris, Christodoulos, primary, Benedetti, Valentina, additional, Novelli, Rubina, additional, Abbate, Mauro, additional, Rizzo, Paola, additional, Conti, Sara, additional, Tomasoni, Susanna, additional, Corna, Daniela, additional, Pozzobon, Michela, additional, Cavallotti, Daniela, additional, Yokoo, Takashi, additional, Morigi, Marina, additional, Benigni, Ariela, additional, and Remuzzi, Giuseppe, additional

Published
2015
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32. Direct Reprogramming of Human Bone Marrow Stromal Cells into Functional Renal Cells Using Cell-free Extracts

Author

Papadimou, Evangelia, primary, Morigi, Marina, additional, Iatropoulos, Paraskevas, additional, Xinaris, Christodoulos, additional, Tomasoni, Susanna, additional, Benedetti, Valentina, additional, Longaretti, Lorena, additional, Rota, Cinzia, additional, Todeschini, Marta, additional, Rizzo, Paola, additional, Introna, Martino, additional, Grazia de Simoni, Maria, additional, Remuzzi, Giuseppe, additional, Goligorsky, Michael S., additional, and Benigni, Ariela, additional

Published
2015
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33. Renal Primordia Activate Kidney Regenerative Events in a Rat Model of Progressive Renal Disease

Author

Imberti, Barbara, primary, Corna, Daniela, additional, Rizzo, Paola, additional, Xinaris, Christodoulos, additional, Abbate, Mauro, additional, Longaretti, Lorena, additional, Cassis, Paola, additional, Benedetti, Valentina, additional, Benigni, Ariela, additional, Zoja, Carlamaria, additional, Remuzzi, Giuseppe, additional, and Morigi, Marina, additional

Published
2015
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36. Human Amniotic Fluid Stem Cell Preconditioning Improves Their Regenerative Potential

Author

Rota, Cinzia, primary, Imberti, Barbara, additional, Pozzobon, Michela, additional, Piccoli, Martina, additional, De Coppi, Paolo, additional, Atala, Anthony, additional, Gagliardini, Elena, additional, Xinaris, Christodoulos, additional, Benedetti, Valentina, additional, Fabricio, Aline S.C., additional, Squarcina, Elisa, additional, Abbate, Mauro, additional, Benigni, Ariela, additional, Remuzzi, Giuseppe, additional, and Morigi, Marina, additional

Published
2012
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38. Study protocol: safety and efficacy of propranolol in newborns with Retinopathy of Prematurity (PROP-ROP): ISRCTN18523491

Author

Filippi, Luca, primary, Cavallaro, Giacomo, additional, Fiorini, Patrizio, additional, Daniotti, Marta, additional, Benedetti, Valentina, additional, Cristofori, Gloria, additional, Araimo, Gabriella, additional, Ramenghi, Luca, additional, La Torre, Agostino, additional, Fortunato, Pina, additional, Pollazzi, Liliana, additional, la Marca, Giancarlo, additional, Malvagia, Sabrina, additional, Bagnoli, Paola, additional, Ristori, Chiara, additional, Dal Monte, Massimo, additional, Bilia, Anna Rita, additional, Isacchi, Benedetta, additional, Furlanetto, Sandra, additional, Tinelli, Francesca, additional, Cioni, Giovanni, additional, Donzelli, Gianpaolo, additional, Osnaghi, Silvia, additional, and Mosca, Fabio, additional

Published
2010
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40. Intracellular accumulation and DNA damage persistence as determinants of human squamous cell carcinoma hypersensitivity to the novel camptothecin ST1968

Author

Pisano, Claudio, primary, Zuco, Valentina, additional, De Cesare, Michelandrea, additional, Benedetti, Valentina, additional, Vesci, Loredana, additional, Foderà, Rosanna, additional, Bucci, Federica, additional, Aulicino, Concetta, additional, Penco, Sergio, additional, Carminati, Paolo, additional, and Zunino, Franco, additional

Published
2008
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43. Engineering kidney tissues for polycystic kidney disease modeling and drug discovery.

Author

Brizi V, Benedetti V, Lavecchia AM, and Xinaris C

Subjects
Animals, Dogs, Drug Evaluation, Preclinical methods, Humans, Kidney Tubules drug effects, Madin Darby Canine Kidney Cells, Polycystic Kidney, Autosomal Dominant drug therapy, Tissue Engineering instrumentation, Tissue Scaffolds, Drug Discovery methods, Kidney Tubules pathology, Polycystic Kidney, Autosomal Dominant pathology, Tissue Engineering methods
Abstract

Autosomal dominant polycystic kidney disease (ADPKD) is the most prevalent, potentially lethal monogenic human disorder. There is currently no cure for ADPKD. The mechanistic complexity of the disease, the absence of animal models that can faithfully mimic the disease, as well as the lack of functional human in vitro assays for compound testing, have made drug discovery for PKD very difficult. We recently developed an engineering platform that allowed us to generate polycystic tubules using patients' own cells to test drug efficacy and discover potential new pharmacological treatments for PKD. Here we describe an engineering platform that enables the generation of custom-made polycystic tubules using patients' own cells for modeling PKD and testing drug efficacy., (© 2019 Elsevier Inc. All rights reserved.)

Published
2019
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44. Generation of Functional Kidney Organoids In Vivo Starting from a Single-Cell Suspension.

Author

Benedetti V, Brizi V, and Xinaris C

Subjects
Cells, Cultured, Humans, Cell Culture Techniques methods, Cell Differentiation, Kidney cytology, Organoids cytology, Pluripotent Stem Cells cytology, Tissue Engineering methods
Abstract

Novel methods in developmental biology and stem cell research have made it possible to generate complex kidney tissues in vitro that resemble whole organs and are termed organoids. In this chapter we describe a technique using suspensions of fully dissociated mouse kidney cells to yield organoids that can become vascularized in vivo and mature and display physiological functions. This system can be used to produce fine-grained human-mouse chimeric organoids in which the renal differentiation potential of human cells can be assessed. It can also be an excellent method for growing chimeric organoids in vivo using human stem cells, which can differentiate into specialized kidney cells and exert nephron-specific functions. We provide detailed methods, a brief discussion of critical points, and describe some successfully implemented examples of the system.

Published
2019
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45. Functional Human Podocytes Generated in Organoids from Amniotic Fluid Stem Cells.

Author

Xinaris C, Benedetti V, Novelli R, Abbate M, Rizzo P, Conti S, Tomasoni S, Corna D, Pozzobon M, Cavallotti D, Yokoo T, Morigi M, Benigni A, and Remuzzi G

Subjects
Animals, Cells, Cultured, Humans, Kidney cytology, Mice, Amniotic Fluid cytology, Organoids cytology, Podocytes, Stem Cells
Abstract

Generating kidney organoids using human stem cells could offer promising prospects for research and therapeutic purposes. However, no cell-based strategy has generated nephrons displaying an intact three-dimensional epithelial filtering barrier. Here, we generated organoids using murine embryonic kidney cells, and documented that these tissues recapitulated the complex three-dimensional filtering structure of glomerular slits in vivo and accomplished selective glomerular filtration and tubular reabsorption. Exploiting this technology, we mixed human amniotic fluid stem cells with mouse embryonic kidney cells to establish three-dimensional chimeric organoids that engrafted in vivo and grew to form vascularized glomeruli and tubular structures. Human cells contributed to the formation of glomerular structures, differentiated into podocytes with slit diaphragms, and internalized exogenously infused BSA, thus attaining in vivo degrees of specialization and function unprecedented for donor stem cells. In conclusion, human amniotic fluid stem cell chimeric organoids may offer new paths for studying renal development and human podocyte disease, and for facilitating drug discovery and translational research., (Copyright © 2016 by the American Society of Nephrology.)

Published
2016
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