13 results on '"Benatti, Thiago R."'
Search Results
2. New Sugarcane Microsatellites and Target Region Amplification Polymorphism Primers Designed from Candidate Genes Related to Disease Resistance
- Author
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dos Santos, Fernanda R. C., Zucchi, Maria I., Park, Jong-Won, Benatti, Thiago R., da Silva, Jorge A., Souza, Glaucia M., Landell, Marcos G. A., and Pinto, Luciana R.
- Published
- 2017
- Full Text
- View/download PDF
3. A neo-sex chromosome that drives postzygotic sex determination in the Hessian Fly (Mayetiola destructor)
- Author
-
Stuart, Jeffrey J., Schemerhorn, Brandon J., Cambron, Sue E., Benatti, Thiago R., Valicente, Fernando H., Aggarwal, Rajat, Chaoyang Zhao, Walling, Jason G., and Ming-Shun Chen
- Subjects
Sex determination, Genetic -- Research ,Chromosome mapping -- Usage ,Sex chromosomes -- Research ,Diptera -- Research ,Biological sciences - Published
- 2010
4. A BAC-based physical map of the Hessian fly genome anchored to polytene chromosomes
- Author
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Fellers John P, Chen Ming-Shun, Zhao Chaoyang, Gill Navdeep, Benatti Thiago R, Aggarwal Rajat, Schemerhorn Brandon J, and Stuart Jeff J
- Subjects
Biotechnology ,TP248.13-248.65 ,Genetics ,QH426-470 - Abstract
Abstract Background The Hessian fly (Mayetiola destructor) is an important insect pest of wheat. It has tractable genetics, polytene chromosomes, and a small genome (158 Mb). Investigation of the Hessian fly presents excellent opportunities to study plant-insect interactions and the molecular mechanisms underlying genome imprinting and chromosome elimination. A physical map is needed to improve the ability to perform both positional cloning and comparative genomic analyses with the fully sequenced genomes of other dipteran species. Results An FPC-based genome wide physical map of the Hessian fly was constructed and anchored to the insect's polytene chromosomes. Bacterial artificial chromosome (BAC) clones corresponding to 12-fold coverage of the Hessian fly genome were fingerprinted, using high information content fingerprinting (HIFC) methodology, and end-sequenced. Fluorescence in situ hybridization (FISH) co-localized two BAC clones from each of the 196 longest contigs on the polytene chromosomes. An additional 70 contigs were positioned using a single FISH probe. The 266 FISH mapped contigs were evenly distributed and covered 60% of the genome (95,668 kb). The ends of the fingerprinted BACs were then sequenced to develop the capacity to create sequenced tagged site (STS) markers on the BACs in the map. Only 3.64% of the BAC-end sequence was composed of transposable elements, helicases, ribosomal repeats, simple sequence repeats, and sequences of low complexity. A relatively large fraction (14.27%) of the BES was comprised of multi-copy gene sequences. Nearly 1% of the end sequence was composed of simple sequence repeats (SSRs). Conclusion This physical map provides the foundation for high-resolution genetic mapping, map-based cloning, and assembly of complete genome sequencing data. The results indicate that restriction fragment length heterogeneity in BAC libraries used to construct physical maps lower the length and the depth of the contigs, but is not an absolute barrier to the successful application of the technology. This map will serve as a genomic resource for accelerating gene discovery, genome sequencing, and the assembly of BAC sequences. The Hessian fly BAC-clone assembly, and the names and positions of the BAC clones used in the FISH experiments are publically available at http://genome.purdue.edu/WebAGCoL/Hfly/WebFPC/.
- Published
- 2009
- Full Text
- View/download PDF
5. New Sugarcane Microsatellites and Target Region Amplification Polymorphism Primers Designed from Candidate Genes Related to Disease Resistance
- Author
-
dos Santos, Fernanda R. C., primary, Zucchi, Maria I., additional, Park, Jong-Won, additional, Benatti, Thiago R., additional, da Silva, Jorge A., additional, Souza, Glaucia M., additional, Landell, Marcos G. A., additional, and Pinto, Luciana R., additional
- Published
- 2016
- Full Text
- View/download PDF
6. A Massive Expansion of Effector Genes Underlies Gall-Formation in the Wheat Pest Mayetiola destructor
- Author
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Zhao, Chaoyang, Escalante, Lucio Navarro, Chen, Hang, Benatti, Thiago R., Qu, Jiaxin, Chellapilla, Sanjay, Waterhouse, Robert M., Wheeler, David, Andersson, Martin N., Bao, Riyue, Batterton, Matthew, Behura, Susanta K., Blankenburg, Kerstin P., Caragea, Doina, Carolan, James C., Coyle, Marcus, El-Bouhssini, Mustapha, Francisco, Liezl, Friedrich, Markus, Gill, Navdeep, Grace, Tony, Grimmelikhuijzen, Cornelis J.P., Han, Yi, Hauser, Frank, Herndon, Nicolae, Holder, Michael, Ioannidis, Panagiotis, Jackson, LaRonda, Javaid, Mehwish, Jhangiani, Shalini N., Johnson, Alisha J., Kalra, Divya, Korchina, Viktoriya, Kovar, Christie, Lara, Fremiet, Lee, Sandra L., Liu, Xuming, Löfstedt, Christer, Mata, Robert, Mathew, Tittu, Muzny, Donna M., Nagar, Swapnil, Nazareth, Lynne V., Okwuonu, Geoffrey, Ongeri, Fiona, Perales, Lora, Peterson, Brittany F., Pu, Ling-Ling, Robertson, Hugh M., Schemerhorn, Brandon J., Scherer, Steven E., Shreve, Jacob T., Simmons, DeNard, Subramanyam, Subhashree, Thornton, Rebecca, Xue, Kun, Weissenberger, George M., Williams, Christie E., Worley, Kim C., Zhu, Dianhui, Zhu, Yiming, Harris, Marion O., Shukle, Richard H., Weren, John H., Zdobnov, Evgeny M., Chen, Ming-Shun, Brown, Susan J., Stuart, Jeffery J., Richards, Stephen, Zhao, Chaoyang, Escalante, Lucio Navarro, Chen, Hang, Benatti, Thiago R., Qu, Jiaxin, Chellapilla, Sanjay, Waterhouse, Robert M., Wheeler, David, Andersson, Martin N., Bao, Riyue, Batterton, Matthew, Behura, Susanta K., Blankenburg, Kerstin P., Caragea, Doina, Carolan, James C., Coyle, Marcus, El-Bouhssini, Mustapha, Francisco, Liezl, Friedrich, Markus, Gill, Navdeep, Grace, Tony, Grimmelikhuijzen, Cornelis J.P., Han, Yi, Hauser, Frank, Herndon, Nicolae, Holder, Michael, Ioannidis, Panagiotis, Jackson, LaRonda, Javaid, Mehwish, Jhangiani, Shalini N., Johnson, Alisha J., Kalra, Divya, Korchina, Viktoriya, Kovar, Christie, Lara, Fremiet, Lee, Sandra L., Liu, Xuming, Löfstedt, Christer, Mata, Robert, Mathew, Tittu, Muzny, Donna M., Nagar, Swapnil, Nazareth, Lynne V., Okwuonu, Geoffrey, Ongeri, Fiona, Perales, Lora, Peterson, Brittany F., Pu, Ling-Ling, Robertson, Hugh M., Schemerhorn, Brandon J., Scherer, Steven E., Shreve, Jacob T., Simmons, DeNard, Subramanyam, Subhashree, Thornton, Rebecca, Xue, Kun, Weissenberger, George M., Williams, Christie E., Worley, Kim C., Zhu, Dianhui, Zhu, Yiming, Harris, Marion O., Shukle, Richard H., Weren, John H., Zdobnov, Evgeny M., Chen, Ming-Shun, Brown, Susan J., Stuart, Jeffery J., and Richards, Stephen
- Abstract
Gall-forming arthropods are highly specialized herbivores that, in combination with their hosts, produce extended phenotypes with unique morphologies [1]. Many are economically important, and others have improved our understanding of ecology and adaptive radiation [2]. However, the mechanisms that these arthropods use to induce plant galls are poorly understood. We sequenced the genome of the Hessian fly (Mayetiola destructor; Diptera: Cecidomyiidae), a plant parasitic gall midge and a pest of wheat (Triticum spp.), with the aim of identifying genic modifications that contribute to its plant-parasitic lifestyle. Among several adaptive modifications, we discovered an expansive reservoir of potential effector proteins. Nearly 5% of the 20,163 predicted gene models matched putative effector gene transcripts present in the M. destructor larval salivary gland. Another 466 putative effectors were discovered among the genes that have no sequence similarities in other organisms. The largest known arthropod gene family (family SSGP-71) was also discovered within the effector reservoir. SSGP-71 proteins lack sequence homologies to other proteins, but their structures resemble both ubiquitin E3 ligases in plants and E3-ligase-mimicking effectors in plant pathogenic bacteria. SSGP-71 proteins and wheat Skp proteins interact in vivo. Mutations in different SSGP-71 genes avoid the effector-triggered immunity that is directed by the wheat resistance genes H6 and H9. Results point to effectors as the agents responsible for arthropod-induced plant gall formation.
- Published
- 2015
7. Cold Responsive Gene Expression Profiling of Sugarcane and Saccharum spontaneum with Functional Analysis of a Cold Inducible Saccharum Homolog of NOD26-Like Intrinsic Protein to Salt and Water Stress
- Author
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Park, Jong-Won, primary, Benatti, Thiago R., additional, Marconi, Thiago, additional, Yu, Qingyi, additional, Solis-Gracia, Nora, additional, Mora, Victoria, additional, and da Silva, Jorge A., additional
- Published
- 2015
- Full Text
- View/download PDF
8. A Massive Expansion of Effector Genes Underlies Gall-Formation in the Wheat Pest Mayetiola destructor
- Author
-
Zhao, Chaoyang, primary, Escalante, Lucio Navarro, additional, Chen, Hang, additional, Benatti, Thiago R., additional, Qu, Jiaxin, additional, Chellapilla, Sanjay, additional, Waterhouse, Robert M., additional, Wheeler, David, additional, Andersson, Martin N., additional, Bao, Riyue, additional, Batterton, Matthew, additional, Behura, Susanta K., additional, Blankenburg, Kerstin P., additional, Caragea, Doina, additional, Carolan, James C., additional, Coyle, Marcus, additional, El-Bouhssini, Mustapha, additional, Francisco, Liezl, additional, Friedrich, Markus, additional, Gill, Navdeep, additional, Grace, Tony, additional, Grimmelikhuijzen, Cornelis J.P., additional, Han, Yi, additional, Hauser, Frank, additional, Herndon, Nicolae, additional, Holder, Michael, additional, Ioannidis, Panagiotis, additional, Jackson, LaRonda, additional, Javaid, Mehwish, additional, Jhangiani, Shalini N., additional, Johnson, Alisha J., additional, Kalra, Divya, additional, Korchina, Viktoriya, additional, Kovar, Christie L., additional, Lara, Fremiet, additional, Lee, Sandra L., additional, Liu, Xuming, additional, Löfstedt, Christer, additional, Mata, Robert, additional, Mathew, Tittu, additional, Muzny, Donna M., additional, Nagar, Swapnil, additional, Nazareth, Lynne V., additional, Okwuonu, Geoffrey, additional, Ongeri, Fiona, additional, Perales, Lora, additional, Peterson, Brittany F., additional, Pu, Ling-Ling, additional, Robertson, Hugh M., additional, Schemerhorn, Brandon J., additional, Scherer, Steven E., additional, Shreve, Jacob T., additional, Simmons, DeNard, additional, Subramanyam, Subhashree, additional, Thornton, Rebecca L., additional, Xue, Kun, additional, Weissenberger, George M., additional, Williams, Christie E., additional, Worley, Kim C., additional, Zhu, Dianhui, additional, Zhu, Yiming, additional, Harris, Marion O., additional, Shukle, Richard H., additional, Werren, John H., additional, Zdobnov, Evgeny M., additional, Chen, Ming-Shun, additional, Brown, Susan J., additional, Stuart, Jeffery J., additional, and Richards, Stephen, additional
- Published
- 2015
- Full Text
- View/download PDF
9. A BAC-Based Physical Map of the Hessian Fly Genome Anchored to Polytene Chromosomes.
- Author
-
Aggarwal, Rajat, Benatti, Thiago R, Gill, Navdeep, Zhao, Chaoyang, Chen, Ming-Shun, Fellers, John P, Schemerhorn, Brandon, Stuart, Jeffrey J, Aggarwal, Rajat, Benatti, Thiago R, Gill, Navdeep, Zhao, Chaoyang, Chen, Ming-Shun, Fellers, John P, Schemerhorn, Brandon, and Stuart, Jeffrey J
- Abstract
Background The Hessian fly (Mayetiola destructor) is an important insect pest of wheat. It has tractable genetics, polytene chromosomes, and a small genome (158 Mb). Investigation of the Hessian fly presents excellent opportunities to study plant-insect interactions and the molecular mechanisms underlying genome imprinting and chromosome elimination. A physical map is needed to improve the ability to perform both positional cloning and comparative genomic analyses with the fully sequenced genomes of other dipteran species. Results An FPC-based genome wide physical map of the Hessian fly was constructed and anchored to the insect's polytene chromosomes. Bacterial artificial chromosome (BAC) clones corresponding to 12-fold coverage of the Hessian fly genome were fingerprinted, using high information content fingerprinting (HIFC) methodology, and end-sequenced. Fluorescence in situ hybridization (FISH) co-localized two BAC clones from each of the 196 longest contigs on the polytene chromosomes. An additional 70 contigs were positioned using a single FISH probe. The 266 FISH mapped contigs were evenly distributed and covered 60% of the genome (95,668 kb). The ends of the fingerprinted BACs were then sequenced to develop the capacity to create sequenced tagged site (STS) markers on the BACs in the map. Only 3.64% of the BAC-end sequence was composed of transposable elements, helicases, ribosomal repeats, simple sequence repeats, and sequences of low complexity. A relatively large fraction (14.27%) of the BES was comprised of multi-copy gene sequences. Nearly 1% of the end sequence was composed of simple sequence repeats (SSRs). Conclusion This physical map provides the foundation for high-resolution genetic mapping, map-based cloning, and assembly of complete genome sequencing data. The results indicate that restriction fragment length heterogeneity in BAC libraries used to construct physical maps lower the length and the depth of the contigs, but is not an absolute barr
- Published
- 2009
10. A Neo-Sex Chromosome That Drives Postzygotic Sex Determination in the Hessian Fly (Mayetiola destructor)
- Author
-
Benatti, Thiago R, primary, Valicente, Fernando H, additional, Aggarwal, Rajat, additional, Zhao, Chaoyang, additional, Walling, Jason G, additional, Chen, Ming-Shun, additional, Cambron, Sue E, additional, Schemerhorn, Brandon J, additional, and Stuart, Jeffrey J, additional
- Published
- 2010
- Full Text
- View/download PDF
11. A BAC-based physical map of the Hessian fly genome anchored to polytene chromosomes
- Author
-
Aggarwal, Rajat, primary, Benatti, Thiago R, additional, Gill, Navdeep, additional, Zhao, Chaoyang, additional, Chen, Ming-Shun, additional, Fellers, John P, additional, Schemerhorn, Brandon J, additional, and Stuart, Jeff J, additional
- Published
- 2009
- Full Text
- View/download PDF
12. Isolation and characterization of microsatellite loci from a commercial cultivar of Musa acuminata
- Author
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CRESTE, SILVANA, primary, BENATTI, THIAGO R., additional, ORSI, MYRIAN R., additional, RISTERUCCI, ANGE‐MARIE, additional, and FIGUEIRA, ANTONIO, additional
- Published
- 2006
- Full Text
- View/download PDF
13. A BAC-based physical map of the Hessian fly genome anchored topolytene chromosomes.
- Author
-
Aggarwal, Rajat, Benatti, Thiago R., Gill, Navdeep, Chaoyang Zhao, Ming- Shun Chen, Fellers, John P., Schemerhorn, Brandon J., and Stuart, Jeff J.
- Subjects
- *
HESSIAN fly , *GENETICS , *GENOMES , *CHROMOSOMES , *CLONING - Abstract
Background: The Hessian fly (Mayetiola destructor) is an important insect pest of wheat. It has tractable genetics, polytene chromosomes, and a small genome (158 Mb). Investigation of the Hessian fly presents excellent opportunities to study plant-insect interactions and the molecular mechanisms underlying genome imprinting and chromosome elimination. A physical map is needed to improve the ability to perform both positional cloning and comparative genomic analyses with the fully sequenced genomes of other dipteran species. Results: An FPC-based genome wide physical map of the Hessian fly was constructed and anchored to the insect's polytene chromosomes. Bacterial artificial chromosome (BAC) clones corresponding to 12-fold coverage of the Hessian fly genome were fingerprinted, using high information content fingerprinting (HIFC) methodology, and end-sequenced. Fluorescence in situ hybridization (FISH) co-localized two BAC clones from each of the 196 longest contigs on the polytene chromosomes. An additional 70 contigs were positioned using a single FISH probe. The 266 FISH mapped contigs were evenly distributed and covered 60% of the genome (95,668 kb). The ends of the fingerprinted BACs were then sequenced to develop the capacity to create sequenced tagged site (STS) markers on the BACs in the map. Only 3.64% of the BAC-end sequence was composed of transposable elements, helicases, ribosomal repeats, simple sequence repeats, and sequences of low complexity. A relatively large fraction (14.27%) of the BES was comprised of multi-copy gene sequences. Nearly 1% of the end sequence was composed of simple sequence repeats (SSRs). Conclusion: This physical map provides the foundation for high-resolution genetic mapping, map-based cloning, and assembly of complete genome sequencing data. The results indicate that restriction fragment length heterogeneity in BAC libraries used to construct physical maps lower the length and the depth of the contigs, but is not an absolute barrier to the successful application of the technology. This map will serve as a genomic resource for accelerating gene discovery, genome sequencing, and the assembly of BAC sequences. The Hessian fly BAC-clone assembly, and the names and positions of the BAC clones used in the FISH experiments are publically available at http://genome.purdue.edu/WebAGCoL/Hfly/WebFPC/. [ABSTRACT FROM AUTHOR]
- Published
- 2009
- Full Text
- View/download PDF
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