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The role of dogs as reservoir hosts for Toscana virus (TOSV) remains undetermined. This study investigated TOSV and Leishmania infantum infections in one healthy and three infected dogs with Leishmania (A, B, C) following natural exposition to sandfly bites in a focus of zoonotic visceral leishmaniasis (ZVL) located in Northern Tunisia from June to October 2020. At the end of the exposition period, infected and healthy dogs were examined for TOSV and L. infantum infections by xenodiagnosis using a colony of Phlebotomus perniciosus. Pools of freshly engorged P. perniciosus at days 0 and those at days 7 post-feeding were screened for TOSV and L. infantum by nested PCR in the polymerase gene and kinetoplast minicircle DNA, respectively. In the exposure site, P. pernicious is the most abundant sandfly species. The infection rates of sandflies with TOSV and L. infantum were 0.10 and 0.05%, respectively. Leishmania infantum DNA and TOSV RNA were detected in P. perniciosus females fed on dog B and C, respectively. The isolation of TOSV in Vero cells was achieved from two pools containing P. perniciosus fed on dog C. No pathogens were detected in P. perniciosus females fed on dog A and on control dog. We report for the first time the reservoir competence of dog with ZVL in the transmission of TOSV to sandfly vectors in natural settings, in addition to its role as a main reservoir host of L. infantum.

Lead dioxide-based electrodes have shown a great performance in the electrochemical treatment of organic wastewater. In the present study, modified PbO2 anodes supported on stainless steel (SS) with a titanium oxide interlayer such as SS/TiO2/PbO2 and SS/TiO2/PbO2-10% Boron (B) were prepared by the sol–gel spin-coating technique. The morphological and structural properties of the prepared electrodes were characterized by scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), and X-ray photoelectron spectroscopy (XPS). It was found that the SS/TiO2/PbO2-10% B anode led to a rougher active surface, larger specific surface area, and therefore stronger ability to generate powerful oxidizing agents. The electrochemical impedance spectroscopy (EIS) measurements showed that the modified PbO2 anodes displayed a lower charge transfer resistance Rct. The influence of the introduction of a TiO2 intermediate layer and the boron doping of a PbO2 active surface layer on the electrochemical degradation of ampicillin (AMP) antibiotic have been investigated by chemical oxygen demand measurements and HPLC analysis. Although HPLC analysis showed that the degradation process of AMP with SS/PbO2 was slightly faster than the modified PbO2 anodes, the results revealed that SS/TiO2/PbO2-10%B was the most efficient and economical anode toward the pollutant degradation due to its physico-chemical properties. At the end of the electrolysis, the chemical oxygen demand (COD), the average current efficiency (ACE) and the energy consumption (EC) reached, respectively, 69.23%, 60.30% and 0.056 kWh (g COD)−1, making SS/TiO2/PbO2-10%B a promising anode for the degradation of ampicillin antibiotic in aqueous solutions.

Epizootic haemorrhagic disease (EHD) is a Culicoides-borne viral disease caused by the epizootic haemorrhagic disease virus (EHDV) associated with clinical manifestations in domestic and wild ruminants, primarily white-tailed deer (Odocoileus virginianus) and cattle (Bos taurus). In late September 2021, EHDV was reported in cattle farms in central/western Tunisia. It rapidly spread throughout the country with more than 200 confirmed outbreaks. We applied a combination of classical and molecular techniques to characterize the causative virus as a member of the serotype EHDV-8. This is the first evidence of EHDV- 8 circulation since 1982 when the prototype EHDV-8 strain was isolated in Australia. This work highlights the urgent need for vaccines for a range of EHDV serotypes.

Féodor Jelenc (1911-2001), an overlooked botanist and bryologist, made a major contribution to the study of bryophytes in North Africa. His work laid the modern foundations for subsequent research on the bryophyte flora of the Maghreb and continues to influence current studies. A professor in Algeria until the country's independence, Féodor Jelenc later ended his career as a professor in Châtellerault. He is the author of several important publications on the flora of the western Massif Central. He was also actively involved in the Esperanto movement. Despite the loss of many original specimens, his herbarium collections and publications remain valuable resources for research in the Maghreb. [ABSTRACT FROM AUTHOR]

Background: Ingrown toenail is a common problem causing significant morbidity. Various procedures used for treating this painful condition were discussed in the literature.Objetctive: Through a prospective study we aimed to evaluate in a long-term follow-up the efficacy of partial nail plate avulsion combined with phenolic chemical matricectomy for the treatment of Ingrown toenails.Methods: We recruited outpatient from January 2006 to December 2009; all treated by phenol matricectomy and assured a regular follow up.Results: Consecutive 146 patients with a total of 171 Ingrown toenails were enrolled. There was no significant morbidity and the procedure was tolerated well by all patients. The short-term results were excellent. No severe complications occurred during the 21-month follow-up period. The overall recurrence was 2.7 %.Conclusion: Phenol cauterization compared with other surgical ablation techniques may be the treatment of choice of Ingrown toenails. It’s an effective technique for the treatment of Ingrown toenails accompanied by less morbidity and lower recurrence rates.

A novel phlebovirus, Punique virus (PUNV), was discovered and isolated in 2008 from sandflies from Northern Tunisia. PUNV is now classified as a unique member of the Punique phlebovirus species within the Phlebovirus genus in the Phenuiviridae family (order bunyavirales). In this study, we aimed to investigate the transmission dynamics of PUNV in Tunisia. Sandflies were collected during two consecutive years, 2009 and 2010, by CDC light traps. In 2009, a total of 873 sandflies were collected and identified to the species level. Phlebotomus perniciosus was the most abundant species. One pool of P. perniciosus females collected in autumn contained PUNV RNA, yielding an infection rate of 0.11%. The population densities of circulating sandfly species were assessed during May–November 2010 in Northern Tunisia by using sticky traps. Phlebotomus (Larroussius) perniciosus (71.74%) was the most abundant species, followed by Phlebotumus (Larroussius) longicuspis (17.47%), and Phlebotumus (Larroussius) perfiliewi (8.82%). The densities of dominant sandfly species were found to peak in early spring and again in the autumn. In 2010, species identification was not performed, and sandflies were only discriminated on the basis of sex and collection date. Out of 249 pools, three contained PUNV RNA. Each positive pool allowed virus isolation. The three pools of female sandflies containing PUNV RNA were collected in autumn with an infection rate of 0.05%. These findings provide further evidence that P. perniciosus is the main vector of PUNV in Tunisia, and this phlebovirus is endemic in Tunisia. Our findings provided strong evidence of intensive circulation of PUNV in sandflies and hosts through a viral infection buildup process between sandfly vectors and hosts starting at the beginning of the activity of sandflies in spring to reach a maximum during the second main peak in autumn.

Due to a high conductivity of about 0.1 S·cm−1, Li-Na-K carbonate eutectic and Sm-doped ceria composite material is a good electrolyte candidate for hybrid fuel cells operating between 500 °C and 600 °C. The present paper aims at a deeper understanding of the species and mechanisms involved in the ionic transport through impedance spectroscopy and thermal analyses, in oxidizing and reducing atmospheres, wet and dry, and during two heating/cooling cycles. Complementary structural analyses of post-mortem phases allowed us to evidence the irreversible partial transformation of molten carbonates into hydrogenated species, when water and/or hydrogen are added in the surrounding atmospheres. Furthermore, this modification was avoided by adding CO2 in anodic and/or cathodic compartments. Finally, a mechanistic model of such composite electrical behavior is suggested, according to the surrounding atmospheres used. It leads to the conclusions that cells based on this kind of electrolyte would preferably operate in molten carbonate fuel cell conditions, than in solid oxide fuel cell conditions, and confirms the name of “Hybrid Fuel Cells” instead of Intermediate Temperature (or even Low Temperature) Solid Oxide Fuel Cells.

Lead dioxide-based electrodes have shown a great performance in the electrochemical treatment of organic wastewater. In the present study, modified PbO2 anodes supported on stainless steel (SS) with a titanium oxide interlayer such as SS/TiO2/PbO2 and SS/TiO2/PbO2-10% Boron (B) were prepared by the sol–gel spin-coating technique. The morphological and structural properties of the prepared electrodes were characterized by scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), and X-ray photoelectron spectroscopy (XPS). It was found that the SS/TiO2/PbO2-10% B anode led to a rougher active surface, larger specific surface area, and therefore stronger ability to generate powerful oxidizing agents. The electrochemical impedance spectroscopy (EIS) measurements showed that the modified PbO2 anodes displayed a lower charge transfer resistance Rct. The influence of the introduction of a TiO2 intermediate layer and the boron doping of a PbO2 active surface layer on the electrochemical degradation of ampicillin (AMP) antibiotic have been investigated by chemical oxygen demand measurements and HPLC analysis. Although HPLC analysis showed that the degradation process of AMP with SS/PbO2 was slightly faster than the modified PbO2 anodes, the results revealed that SS/TiO2/PbO2-10%B was the most efficient and economical anode toward the pollutant degradation due to its physico-chemical properties. At the end of the electrolysis, the chemical oxygen demand (COD), the average current efficiency (ACE) and the energy consumption (EC) reached, respectively, 69.23%, 60.30% and 0.056 kWh (g COD)−1, making SS/TiO2/PbO2-10%B a promising anode for the degradation of ampicillin antibiotic in aqueous solutions. [ABSTRACT FROM AUTHOR]

Epizootic haemorrhagic disease (EHD) is a Culicoides-borne viral disease caused by the epizootic haemorrhagic disease virus (EHDV) associated with clinical manifestations in domestic and wild ruminants, primarily white-tailed deer (Odocoileus virginianus) and cattle (Bos taurus). In late September 2021, EHDV was reported in cattle farms in central/western Tunisia. It rapidly spread throughout the country with more than 200 confirmed outbreaks. We applied a combination of classical and molecular techniques to characterize the causative virus as a member of the serotype EHDV-8. This is the first evidence of EHDV- 8 circulation since 1982 when the prototype EHDV-8 strain was isolated in Australia. This work highlights the urgent need for vaccines for a range of EHDV serotypes. [ABSTRACT FROM AUTHOR]