340 results on '"Bei R."'
Search Results
2. Rapid measurement of total non-structural carbohydrate concentration in grapevine trunk and leaf tissues using near infrared spectroscopy
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De Bei, R., Fuentes, S., Sullivan, W., Edwards, E.J., Tyerman, S., and Cozzolino, D.
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- 2017
- Full Text
- View/download PDF
3. Antitumoral effects of Bortezomib in malignant mesothelioma: evidence of mild endoplasmic reticulum stress in vitro and activation of T cell response in vivo
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Benvenuto, M, Angiolini, V, Focaccetti, C, Nardozi, D, Palumbo, C, Carrano, R, Rufini, A, Bei, R, Miele, Mt, Mancini, P, Barillari, G, Cirone, M, Ferretti, E, Tundo, Gr, Mutti, L, and Masuelli, L
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Proteasome ,Applied Mathematics ,Immunology ,Settore MED/04 ,Settore MED/05 ,General Biochemistry, Genetics and Molecular Biology ,Bortezomib ,Mice ,Modeling and Simulation ,ER stress ,Malignant mesothelioma ,General Agricultural and Biological Sciences ,Ecology, Evolution, Behavior and Systematics - Abstract
Background Malignant mesothelioma (MM) is a rare tumor with a dismal prognosis. The low efficacy of current treatment options highlights the urge to identify more effective therapies aimed at improving MM patients’ survival. Bortezomib (Bor) is a specific and reversible inhibitor of the chymotrypsin-like activity of the 20S core of the proteasome, currently approved for the treatment of multiple myeloma and mantle cell lymphoma. On the other hand, Bor appears to have limited clinical effects on solid tumors, because of its low penetration and accumulation into tumor tissues following intravenous administration. These limitations could be overcome in MM through intracavitary delivery, with the advantage of increasing local drug concentration and decreasing systemic toxicity. Methods In this study, we investigated the effects of Bor on cell survival, cell cycle distribution and modulation of apoptotic and pro-survival pathways in human MM cell lines of different histotypes cultured in vitro. Further, using a mouse MM cell line that reproducibly forms ascites when intraperitoneally injected in syngeneic C57BL/6 mice, we investigated the effects of intraperitoneal Bor administration in vivo on both tumor growth and the modulation of the tumor immune microenvironment. Results We demonstrate that Bor inhibited MM cell growth and induced apoptosis. Further, Bor activated the Unfolded Protein Response, which however appeared to participate in lowering cells’ sensitivity to the drug’s cytotoxic effects. Bor also affected the expression of EGFR and ErbB2 and the activation of downstream pro-survival signaling effectors, including ERK1/2 and AKT. In vivo, Bor was able to suppress MM growth and extend mice survival. The Bor-mediated delay of tumor progression was sustained by increased activation of T lymphocytes recruited to the tumor microenvironment. Conclusions The results presented herein support the use of Bor in MM and advocate future studies aimed at defining the therapeutic potential of Bor and Bor-based combination regimens for this treatment-resistant, aggressive tumor.
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- 2023
4. Recent findings on the impact of ErbB receptors status on prognosis and therapy of head and neck squamous cell carcinoma
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Palumbo, C, Benvenuto, M, Focaccetti, C, Albonici, L, Cifaldi, L, Rufini, A, Nardozi, D, Angiolini, V, Bei, A, Masuelli, L, and Bei, R
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General Medicine ,Settore MED/04 - Abstract
Head and neck squamous cell carcinoma (HNSCC) is the sixth most common cancer type, has often an aggressive course and is poorly responsive to current therapeutic approaches, so that 5-year survival rates for patients diagnosed with advanced disease is lower than 50%. The Epidermal Growth Factor Receptor (EGFR) has emerged as an established oncogene in HNSCC. Indeed, although HNSCCs are a heterogeneous group of cancers which differ for histological, molecular and clinical features, EGFR is overexpressed or mutated in a percentage of cases up to about 90%. Moreover, aberrant expression of the other members of the ErbB receptor family, ErbB2, ErbB3 and ErbB4, has also been reported in variable proportions of HNSCCs. Therefore, an increased expression/activity of one or multiple ErbB receptors is found in the vast majority of patients with HNSCC. While aberrant ErbB signaling has long been known to play a critical role in tumor growth, angiogenesis, invasion, metastatization and resistance to therapy, more recent evidence has revealed its impact on other features of cancer cells’ biology, such as the ability to evade antitumor immunity. In this paper we will review recent findings on how ErbB receptors expression and activity, including that associated with non-canonical signaling mechanisms, impacts on prognosis and therapy of HNSCC.
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- 2023
5. Different outcome of targeting ERN1/IRE1 alpha and EIF2AK3/PERK in NSG mice engrafted with primary effusion lymphoma
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Arena, A, Romeo Maria Anele, Focaccetti, C, Anastasiadou, E, Trived, P, Cifaldi, L, Gilardini, M, Maria, S, Benedetti, R, Santarelli, R, Gonnella, R, Benvenuto, M, Marchese, C, Masuelli, L, Bei, R, and Cirone, M
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Settore MED/04 - Published
- 2023
6. Effect of vine age on vine performance of Shiraz in the Barossa Valley, Australia
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Grigg, D., Methven, D., de Bei, R., Rodríguez López, C.M., Dry, P., and Collins, C.
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- 2018
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7. Molecular Imaging of Mononuclear Cell Mediated Inflammation: From Histopathological Findings to Clinical Practice
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Signore, A., Annovazzi, A., Chianelli, M., Bei, R., Moisan, A., Modesti, A., Cox, Peter H., editor, and Braga, Francisco José Hossri Nogueira
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- 2002
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8. Editorial: The Impact of Angiogenic Growth Factors and Hypoxia on the Antitumor Immune Response
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Albonici, L, Labiano Almiñana, S, Tanaka, T, and Bei, R
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VEGF-Rs ,Immunology ,Immunity ,Tumor Microenvironment ,Immunology and Allergy ,Humans ,vascular endothelial growth factor (VEGF) ,hypoxia ,adaptive immunity ,tumor microenvironment (TME) ,innate immunity ,Settore MED/04 ,Hypoxia - Published
- 2022
9. De novo DHDDS variants cause a neurodevelopmental and neurodegenerative disorder with myoclonus
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Galosi, S., Edani, B.H., Martinelli, S., Hansikova, H., Eklund, E.A., Caputi, C., Masuelli, L., Corsten-Janssen, N., Srour, M., Oegema, R., Bosch, D.G.M., Ellis, C.A., Amlie-Wolf, L., Accogli, A., Atallah, I., Averdunk, L., Barañano, K.W., Bei, R., Bagnasco, I., Brusco, A., Demarest, S., Alaix, A.S., Bonaventura, C. Di, Distelmaier, F., Elmslie, F., Gan-Or, Z., Good, J.M., Gripp, K., Kamsteeg, E.J., Macnamara, E., Marcelis, C.L.M., Mercier, N., Peeden, J., Pizzi, S., Pannone, L., Shinawi, M., Toro, C., Verbeek, N.E., Venkateswaran, S., Wheeler, P.G., Zdrazilova, L., Zhang, R., Zorzi, G., Guerrini, R., Sessa, W.C., Lefeber, D.J., Tartaglia, M., Hamdan, F.F., Grabińska, K.A., Leuzzi, V., Galosi, S., Edani, B.H., Martinelli, S., Hansikova, H., Eklund, E.A., Caputi, C., Masuelli, L., Corsten-Janssen, N., Srour, M., Oegema, R., Bosch, D.G.M., Ellis, C.A., Amlie-Wolf, L., Accogli, A., Atallah, I., Averdunk, L., Barañano, K.W., Bei, R., Bagnasco, I., Brusco, A., Demarest, S., Alaix, A.S., Bonaventura, C. Di, Distelmaier, F., Elmslie, F., Gan-Or, Z., Good, J.M., Gripp, K., Kamsteeg, E.J., Macnamara, E., Marcelis, C.L.M., Mercier, N., Peeden, J., Pizzi, S., Pannone, L., Shinawi, M., Toro, C., Verbeek, N.E., Venkateswaran, S., Wheeler, P.G., Zdrazilova, L., Zhang, R., Zorzi, G., Guerrini, R., Sessa, W.C., Lefeber, D.J., Tartaglia, M., Hamdan, F.F., Grabińska, K.A., and Leuzzi, V.
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Item does not contain fulltext
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- 2022
10. Phenotypic and genetic spectrum of ATP6V1A encephalopathy:a disorder of lysosomal homeostasis
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Guerrini, R. (Renzo), Mei, D. (Davide), Kerti-Szigeti, K. (Katalin), Pepe, S. (Sara), Koenig, M. K. (Mary Kay), Von Allmen, G. (Gretchen), Cho, M. T. (Megan T), McDonald, K. (Kimberly), Baker, J. (Janice), Bhambhani, V. (Vikas), Powis, Z. (Zöe), Rodan, L. (Lance), Nabbout, R. (Rima), Barcia, G. (Giulia), Rosenfeld, J. A. (Jill A), Bacino, C. A. (Carlos A), Mignot, C. (Cyril), Power, L. H. (Lillian H), Harris, C. J. (Catharine J), Marjanovic, D. (Dragan), Møller, R. S. (Rikke S), Hammer, T. B. (Trine B), T. D. (The DDD Study), Keski Filppula, R. (Riikka), Vieira, P. (Päivi), Hildebrandt, C. (Clara), Sacharow, S. (Stephanie), U. D. (Undiagnosed Diseases Network), Maragliano, L. (Luca), Benfenati, F. (Fabio), Lachlan, K. (Katherine), Benneche, A. (Andreas), Petit, F. (Florence), de Sainte Agathe, J. M. (Jean Madeleine), Hallinan, B. (Barbara), Si, Y. (Yue), Wentzensen, I. M. (Ingrid M), Zou, F. (Fanggeng), Narayanan, V. (Vinodh), Matsumoto, N. (Naomichi), Boncristiano, A. (Alessandra), la Marca, G. (Giancarlo), Kato, M. (Mitsuhiro), Anderson, K. (Kristin), Barba, C. (Carmen), Sturiale, L. (Luisa), Garozzo, D. (Domenico), Bei, R. (Roberto), A. c. (ATP6V1A collaborators), Masuelli, L. (Laura), Conti, V. (Valerio), Novarino, G. (Gaia), and Fassio, A. (Anna)
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developmental delay ,epileptic encephalopathy ,lysosomal disorder ,ATP6V1A ,progressive brain atrophy - Abstract
Vacuolar-type H⁺-ATPase (V-ATPase) is a multimeric complex present in a variety of cellular membranes that acts as an ATP-dependent proton pump and plays a key role in pH homeostasis and intracellular signalling pathways. In humans, 22 autosomal genes encode for a redundant set of subunits allowing the composition of diverse V-ATPase complexes with specific properties and expression. Sixteen subunits have been linked to human disease. Here we describe 26 patients harbouring 20 distinct pathogenic de novo missense ATP6V1A variants, mainly clustering within the ATP synthase α/β family-nucleotide-binding domain. At a mean age of 7 years (extremes: 6 weeks, youngest deceased patient to 22 years, oldest patient) clinical pictures included early lethal encephalopathies with rapidly progressive massive brain atrophy, severe developmental epileptic encephalopathies and static intellectual disability with epilepsy. The first clinical manifestation was early hypotonia, in 70%; 81% developed epilepsy, manifested as developmental epileptic encephalopathies in 58% of the cohort and with infantile spasms in 62%; 63% of developmental epileptic encephalopathies failed to achieve any developmental, communicative or motor skills. Less severe outcomes were observed in 23% of patients who, at a mean age of 10 years and 6 months, exhibited moderate intellectual disability, with independent walking and variable epilepsy. None of the patients developed communicative language. Microcephaly (38%) and amelogenesis imperfecta/enamel dysplasia (42%) were additional clinical features. Brain MRI demonstrated hypomyelination and generalized atrophy in 68%. Atrophy was progressive in all eight individuals undergoing repeated MRIs. Fibroblasts of two patients with developmental epileptic encephalopathies showed decreased LAMP1 expression, Lysotracker staining and increased organelle pH, consistent with lysosomal impairment and loss of V-ATPase function. Fibroblasts of two patients with milder disease, exhibited a different phenotype with increased Lysotracker staining, decreased organelle pH and no significant modification in LAMP1 expression. Quantification of substrates for lysosomal enzymes in cellular extracts from four patients revealed discrete accumulation. Transmission electron microscopy of fibroblasts of four patients with variable severity and of induced pluripotent stem cell-derived neurons from two patients with developmental epileptic encephalopathies showed electron-dense inclusions, lipid droplets, osmiophilic material and lamellated membrane structures resembling phospholipids. Quantitative assessment in induced pluripotent stem cell-derived neurons identified significantly smaller lysosomes. ATP6V1A-related encephalopathy represents a new paradigm among lysosomal disorders. It results from a dysfunctional endo-lysosomal membrane protein causing altered pH homeostasis. Its pathophysiology implies intracellular accumulation of substrates whose composition remains unclear, and a combination of developmental brain abnormalities and neurodegenerative changes established during prenatal and early postanal development, whose severity is variably determined by specific pathogenic variants.
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- 2022
11. A common repertoire of autoantibodies is shared by cancer and autoimmune disease patients: Inflammation in their induction and impact on tumor growth
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Bei, R., Masuelli, L., Palumbo, C., Modesti, M., and Modesti, A.
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- 2009
- Full Text
- View/download PDF
12. Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)
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Klionsky, DJ, Abdel-Aziz, AK, Abdelfatah, S, Abdellatif, M, Abdoli, A, Abel, S, Abeliovich, H, Abildgaard, MH, Abudu, YP, Acevedo-Arozena, A, Adamopoulos, IE, Adeli, K, Adolph, TE, Adornetto, A, Aflaki, E, Agam, G, Agarwal, A, Aggarwal, BB, Agnello, M, Agostinis, P, Agrewala, JN, Agrotis, A, Aguilar, PV, Ahmad, ST, Ahmed, ZM, Ahumada-Castro, U, Aits, S, Aizawa, S, Akkoc, Y, Akoumianaki, T, Akpinar, HA, Al-Abd, AM, Al-Akra, L, Al-Gharaibeh, A, Alaoui-Jamali, MA, Alberti, S, Alcocer-Gómez, E, Alessandri, C, Ali, M, Alim Al-Bari, MA, Aliwaini, S, Alizadeh, J, Almacellas, E, Almasan, A, Alonso, A, Alonso, GD, Altan-Bonnet, N, Altieri, DC, Álvarez, ÉMC, Alves, S, Alves da Costa, C, Alzaharna, MM, Amadio, M, Amantini, C, Amaral, C, Ambrosio, S, Amer, AO, Ammanathan, V, An, Z, Andersen, SU, Andrabi, SA, Andrade-Silva, M, Andres, AM, Angelini, S, Ann, D, Anozie, UC, Ansari, MY, Antas, P, Antebi, A, Antón, Z, Anwar, T, Apetoh, L, Apostolova, N, Araki, T, Araki, Y, Arasaki, K, Araújo, WL, Araya, J, Arden, C, Arévalo, M-A, Arguelles, S, Arias, E, Arikkath, J, Arimoto, H, Ariosa, AR, Armstrong-James, D, Arnauné-Pelloquin, L, Aroca, A, Arroyo, DS, Arsov, I, Artero, R, Asaro, DML, Aschner, M, Ashrafizadeh, M, Ashur-Fabian, O, Atanasov, AG, Au, AK, Auberger, P, Auner, HW, Aurelian, L, Autelli, R, Avagliano, L, Ávalos, Y, Aveic, S, Aveleira, CA, Avin-Wittenberg, T, Aydin, Y, Ayton, S, Ayyadevara, S, Azzopardi, M, Baba, M, Backer, JM, Backues, SK, Bae, D-H, Bae, O-N, Bae, SH, Baehrecke, EH, Baek, A, Baek, S-H, Baek, SH, Bagetta, G, Bagniewska-Zadworna, A, Bai, H, Bai, J, Bai, X, Bai, Y, Bairagi, N, Baksi, S, Balbi, T, Baldari, CT, Balduini, W, Ballabio, A, Ballester, M, Balazadeh, S, Balzan, R, Bandopadhyay, R, Banerjee, S, Bánréti, Á, Bao, Y, Baptista, MS, Baracca, A, Barbati, C, Bargiela, A, Barilà, D, Barlow, PG, Barmada, SJ, Barreiro, E, Barreto, GE, Bartek, J, Bartel, B, Bartolome, A, Barve, GR, Basagoudanavar, SH, Bassham, DC, Bast, RC, Basu, A, Batoko, H, Batten, I, Baulieu, EE, Baumgarner, BL, Bayry, J, Beale, R, Beau, I, Beaumatin, F, Bechara, LRG, Beck, GR, Beers, MF, Begun, J, Behrends, C, Behrens, GMN, Bei, R, Bejarano, E, Bel, S, Behl, C, Belaid, A, Belgareh-Touzé, N, Bellarosa, C, Belleudi, F, Belló Pérez, M, Bello-Morales, R, Beltran, JSDO, Beltran, S, Benbrook, DM, Bendorius, M, Benitez, BA, Benito-Cuesta, I, Bensalem, J, Berchtold, MW, Berezowska, S, Bergamaschi, D, Bergami, M, Bergmann, A, Berliocchi, L, Berlioz-Torrent, C, Bernard, A, Berthoux, L, Besirli, CG, Besteiro, S, Betin, VM, Beyaert, R, Bezbradica, JS, Bhaskar, K, Bhatia-Kissova, I, Bhattacharya, R, Bhattacharya, S, Bhattacharyya, S, Bhuiyan, MS, Bhutia, SK, Bi, L, Bi, X, Biden, TJ, Bijian, K, Billes, VA, Binart, N, Bincoletto, C, Birgisdottir, AB, Bjorkoy, G, Blanco, G, Blas-Garcia, A, Blasiak, J, Blomgran, R, Blomgren, K, Blum, JS, Boada-Romero, E, Boban, M, Boesze-Battaglia, K, Boeuf, P, Boland, B, Bomont, P, Bonaldo, P, Bonam, SR, Bonfili, L, Bonifacino, JS, Boone, BA, Bootman, MD, Bordi, M, Borner, C, Bornhauser, BC, Borthakur, G, Bosch, J, Bose, S, Botana, LM, Botas, J, Boulanger, CM, Boulton, ME, Bourdenx, M, Bourgeois, B, Bourke, NM, Bousquet, G, Boya, P, Bozhkov, PV, Bozi, LHM, Bozkurt, TO, Brackney, DE, Brandts, CH, Braun, RJ, Braus, GH, Bravo-Sagua, R, Bravo-San Pedro, JM, Brest, P, Bringer, M-A, Briones-Herrera, A, Broaddus, VC, Brodersen, P, Brodsky, JL, Brody, SL, Bronson, PG, Bronstein, JM, Brown, CN, Brown, RE, Brum, PC, Brumell, JH, Brunetti-Pierri, N, Bruno, D, Bryson-Richardson, RJ, Bucci, C, Buchrieser, C, Bueno, M, Buitrago-Molina, LE, Buraschi, S, Buch, S, Buchan, JR, Buckingham, EM, Budak, H, Budini, M, Bultynck, G, Burada, F, Burgoyne, JR, Burón, MI, Bustos, V, Büttner, S, Butturini, E, Byrd, A, Cabas, I, Cabrera-Benitez, S, Cadwell, K, Cai, J, Cai, L, Cai, Q, Cairó, M, Calbet, JA, Caldwell, GA, Caldwell, KA, Call, JA, Calvani, R, Calvo, AC, Calvo-Rubio Barrera, M, Camara, NO, Camonis, JH, Camougrand, N, Campanella, M, Campbell, EM, Campbell-Valois, F-X, Campello, S, Campesi, I, Campos, JC, Camuzard, O, Cancino, J, Candido de Almeida, D, Canesi, L, Caniggia, I, Canonico, B, Cantí, C, Cao, B, Caraglia, M, Caramés, B, Carchman, EH, Cardenal-Muñoz, E, Cardenas, C, Cardenas, L, Cardoso, SM, Carew, JS, Carle, GF, Carleton, G, Carloni, S, Carmona-Gutierrez, D, Carneiro, LA, Carnevali, O, Carosi, JM, Carra, S, Carrier, A, Carrier, L, Carroll, B, Carter, AB, Carvalho, AN, Casanova, M, Casas, C, Casas, J, Cassioli, C, Castillo, EF, Castillo, K, Castillo-Lluva, S, Castoldi, F, Castori, M, Castro, AF, Castro-Caldas, M, Castro-Hernandez, J, Castro-Obregon, S, Catz, SD, Cavadas, C, Cavaliere, F, Cavallini, G, Cavinato, M, Cayuela, ML, Cebollada Rica, P, Cecarini, V, Cecconi, F, Cechowska-Pasko, M, Cenci, S, Ceperuelo-Mallafré, V, Cerqueira, JJ, Cerutti, JM, Cervia, D, Cetintas, VB, Cetrullo, S, Chae, H-J, Chagin, AS, Chai, C-Y, Chakrabarti, G, Chakrabarti, O, Chakraborty, T, Chami, M, Chamilos, G, Chan, DW, Chan, EYW, Chan, ED, Chan, HYE, Chan, HH, Chan, H, Chan, MTV, Chan, YS, Chandra, PK, Chang, C-P, Chang, C, Chang, H-C, Chang, K, Chao, J, Chapman, T, Charlet-Berguerand, N, Chatterjee, S, Chaube, SK, Chaudhary, A, Chauhan, S, Chaum, E, Checler, F, Cheetham, ME, Chen, C-S, Chen, G-C, Chen, J-F, Chen, LL, Chen, L, Chen, M, Chen, M-K, Chen, N, Chen, Q, Chen, R-H, Chen, S, Chen, W, Chen, X-M, Chen, X-W, Chen, X, Chen, Y, Chen, Y-G, Chen, Y-J, Chen, Y-Q, Chen, ZS, Chen, Z, Chen, Z-H, Chen, ZJ, Cheng, H, Cheng, J, Cheng, S-Y, Cheng, W, Cheng, X, Cheng, X-T, Cheng, Y, Cheng, Z, Cheong, H, Cheong, JK, Chernyak, BV, Cherry, S, Cheung, CFR, Cheung, CHA, Cheung, K-H, Chevet, E, Chi, RJ, Chiang, AKS, Chiaradonna, F, Chiarelli, R, Chiariello, M, Chica, N, Chiocca, S, Chiong, M, Chiou, S-H, Chiramel, AI, Chiurchiù, V, Cho, D-H, Choe, S-K, Choi, AMK, Choi, ME, Choudhury, KR, Chow, NS, Chu, CT, Chua, JP, Chua, JJE, Chung, H, Chung, KP, Chung, S, Chung, S-H, Chung, Y-L, Cianfanelli, V, Ciechomska, IA, Cifuentes, M, Cinque, L, Cirak, S, Cirone, M, Clague, MJ, Clarke, R, Clementi, E, Coccia, EM, Codogno, P, Cohen, E, Cohen, MM, Colasanti, T, Colasuonno, F, Colbert, RA, Colell, A, Čolić, M, Coll, NS, Collins, MO, Colombo, MI, Colón-Ramos, DA, Combaret, L, Comincini, S, Cominetti, MR, Consiglio, A, Conte, A, Conti, F, Contu, VR, Cookson, MR, Coombs, KM, Coppens, I, Corasaniti, MT, Corkery, DP, Cordes, N, Cortese, K, Costa, MDC, Costantino, S, Costelli, P, Coto-Montes, A, Crack, PJ, Crespo, JL, Criollo, A, Crippa, V, Cristofani, R, Csizmadia, T, Cuadrado, A, Cui, B, Cui, J, Cui, Y, Culetto, E, Cumino, AC, Cybulsky, AV, Czaja, MJ, Czuczwar, SJ, D'Adamo, S, D'Amelio, M, D'Arcangelo, D, D'Lugos, AC, D'Orazi, G, da Silva, JA, Dafsari, HS, Dagda, RK, Dagdas, Y, Daglia, M, Dai, X, Dai, Y, Dal Col, J, Dalhaimer, P, Dalla Valle, L, Dallenga, T, Dalmasso, G, Damme, M, Dando, I, Dantuma, NP, Darling, AL, Das, H, Dasarathy, S, Dasari, SK, Dash, S, Daumke, O, Dauphinee, AN, Davies, JS, Dávila, VA, Davis, RJ, Davis, T, Dayalan Naidu, S, De Amicis, F, De Bosscher, K, De Felice, F, De Franceschi, L, De Leonibus, C, de Mattos Barbosa, MG, De Meyer, GRY, De Milito, A, De Nunzio, C, De Palma, C, De Santi, M, De Virgilio, C, De Zio, D, Debnath, J, DeBosch, BJ, Decuypere, J-P, Deehan, MA, Deflorian, G, DeGregori, J, Dehay, B, Del Rio, G, Delaney, JR, Delbridge, LMD, Delorme-Axford, E, Delpino, MV, Demarchi, F, Dembitz, V, Demers, ND, Deng, H, Deng, Z, Dengjel, J, Dent, P, Denton, D, DePamphilis, ML, Der, CJ, Deretic, V, Descoteaux, A, Devis, L, Devkota, S, Devuyst, O, Dewson, G, Dharmasivam, M, Dhiman, R, di Bernardo, D, Di Cristina, M, Di Domenico, F, Di Fazio, P, Di Fonzo, A, Di Guardo, G, Di Guglielmo, GM, Di Leo, L, Di Malta, C, Di Nardo, A, Di Rienzo, M, Di Sano, F, Diallinas, G, Diao, J, Diaz-Araya, G, Díaz-Laviada, I, Dickinson, JM, Diederich, M, Dieudé, M, Dikic, I, Ding, S, Ding, W-X, Dini, L, Dinić, J, Dinic, M, Dinkova-Kostova, AT, Dionne, MS, Distler, JHW, Diwan, A, Dixon, IMC, Djavaheri-Mergny, M, Dobrinski, I, Dobrovinskaya, O, Dobrowolski, R, Dobson, RCJ, Đokić, J, Dokmeci Emre, S, Donadelli, M, Dong, B, Dong, X, Dong, Z, Dorn Ii, GW, Dotsch, V, Dou, H, Dou, J, Dowaidar, M, Dridi, S, Drucker, L, Du, A, Du, C, Du, G, Du, H-N, Du, L-L, du Toit, A, Duan, S-B, Duan, X, Duarte, SP, Dubrovska, A, Dunlop, EA, Dupont, N, Durán, RV, Dwarakanath, BS, Dyshlovoy, SA, Ebrahimi-Fakhari, D, Eckhart, L, Edelstein, CL, Efferth, T, Eftekharpour, E, Eichinger, L, Eid, N, Eisenberg, T, Eissa, NT, Eissa, S, Ejarque, M, El Andaloussi, A, El-Hage, N, El-Naggar, S, Eleuteri, AM, El-Shafey, ES, Elgendy, M, Eliopoulos, AG, Elizalde, MM, Elks, PM, Elsasser, H-P, Elsherbiny, ES, Emerling, BM, Emre, NCT, Eng, CH, Engedal, N, Engelbrecht, A-M, Engelsen, AST, Enserink, JM, Escalante, R, Esclatine, A, Escobar-Henriques, M, Eskelinen, E-L, Espert, L, Eusebio, M-O, Fabrias, G, Fabrizi, C, Facchiano, A, Facchiano, F, Fadeel, B, Fader, C, Faesen, AC, Fairlie, WD, Falcó, A, Falkenburger, BH, 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- Abstract
In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field.
- Published
- 2021
13. Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition).
- Author
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Quadrilatero J., Walker D.W., Walker M.J., Walker S.A., Walter J., Wandosell F.G., Wang B., Wang C., Wang C.-Y., Wang D., Wang F., Wang G., Wang H., Wang H.-G., Wang J., Wang K., Wang L., Wang M.H., Wang M., Wang N., Wang P., Wang Q.J., Wang Q., Wang Q.K., Wang Q.A., Wang W.-T., Wang W., Wang X., Wang Y.-Y., Wang Y., Wang Z., Warnes G., Warnsmann V., Watada H., Watanabe E., Watchon M., Wawrzynska A., Weaver T.E., Wegrzyn G., Wehman A.M., Wei H., Wei L., Wei T., Wei Y., Weiergraber O.H., Weihl C.C., Weindl G., Weiskirchen R., Wells A., Wen R.H., Wen X., Werner A., Weykopf B., Wheatley S.P., W... [Ovid has truncated this field to avoid Excel character limitations], Klionsky D.J., Abdel-Aziz A.K., Abdelfatah S., Abdellatif M., Abdoli A., Abel S., Abeliovich H., Abildgaard M.H., Abudu Y.P., Acevedo-Arozena A., Adamopoulos I.E., Adeli K., Adolph T.E., Adornetto A., Aflaki E., Agam G., Agarwal A., Aggarwal B.B., Agnello M., Agostinis P., Agrewala J.N., Agrotis A., Aguilar P.V., Ahmad S.T., Ahmed Z.M., Ahumada-Castro U., Aits S., Aizawa S., Akkoc Y., Akoumianaki T., Akpinar H.A., Al-Abd A.M., Al-Akra L., Al-Gharaibeh A., Alaoui-Jamali M.A., Alberti S., Alcocer-Gomez E., Alessandri C., Ali M., Alim Al-Bari M.A., Aliwaini S., Alizadeh J., Almacellas E., Almasan A., Alonso A., Alonso G.D., Altan-Bonnet N., Altieri D.C., Alvarez E.M.C., Alves S., Alves da Costa C., Alzaharna M.M., Amadio M., Amantini C., Amaral C., Ambrosio S., Amer A.O., Ammanathan V., An Z., Andersen S.U., Andrabi S.A., Andrade-Silva M., Andres A.M., Angelini S., Ann D., Anozie U.C., Ansari M.Y., Antas P., Antebi A., Anton Z., Anwar T., Apetoh L., Apostolova N., Araki T., Araki Y., Arasaki K., Araujo W.L., Araya J., Arden C., Arevalo M.-A., Arguelles S., Arias E., Arikkath J., Arimoto H., Ariosa A.R., Armstrong-James D., Arnaune-Pelloquin L., Aroca A., Arroyo D.S., Arsov I., Artero R., Asaro D.M.L., Aschner M., Ashrafizadeh M., Ashur-Fabian O., Atanasov A.G., Au A.K., Auberger P., Auner H.W., Aurelian L., Autelli R., Avagliano L., Avalos Y., Aveic S., Aveleira C.A., Avin-Wittenberg T., Aydin Y., Ayton S., Ayyadevara S., Azzopardi M., Baba M., Backer J.M., Backues S.K., Bae D.-H., Bae O.-N., Bae S.H., Baehrecke E.H., Baek A., Baek S.-H., Baek S.H., Bagetta G., Bagniewska-Zadworna A., Bai H., Bai J., Bai X., Bai Y., Bairagi N., Baksi S., Balbi T., Baldari C.T., Balduini W., Ballabio A., Ballester M., Balazadeh S., Balzan R., Bandopadhyay R., Banerjee S., Banreti A., Bao Y., Baptista M.S., Baracca A., Barbati C., Bargiela A., Barila D., Barlow P.G., Barmada S.J., Barreiro E., Barreto G.E., Bartek J., Bartel B., Bartolome A., Barve G.R., Basagoudanavar S.H., Bassham D.C., Bast R.C., Basu A., Batoko H., Batten I., Baulieu E.E., Baumgarner B.L., Bayry J., Beale R., Beau I., Beaumatin F., Bechara L.R.G., Beck G.R., Beers M.F., Begun J., Behrends C., Behrens G.M.N., Bei R., Bejarano E., Bel S., Behl C., Belaid A., Belgareh-Touze N., Bellarosa C., Belleudi F., Bello Perez M., Bello-Morales R., Beltran J.S.D.O., Beltran S., Benbrook D.M., Bendorius M., Benitez B.A., Benito-Cuesta I., Bensalem J., Berchtold M.W., Berezowska S., Bergamaschi D., Bergami M., Bergmann A., Berliocchi L., Berlioz-Torrent C., Bernard A., Berthoux L., Besirli C.G., Besteiro S., Betin V.M., Beyaert R., Bezbradica J.S., Bhaskar K., Bhatia-Kissova I., Bhattacharya R., Bhattacharya S., Bhattacharyya S., Bhuiyan M.S., Bhutia S.K., Bi L., Bi X., Biden T.J., Bijian K., Billes V.A., Binart N., Bincoletto C., Birgisdottir A.B., Bjorkoy G., Blanco G., Blas-Garcia A., Blasiak J., Blomgran R., Blomgren K., Blum J.S., Boada-Romero E., Boban M., Boesze-Battaglia K., Boeuf P., Boland B., Bomont P., Bonaldo P., Bonam S.R., Bonfili L., Bonifacino J.S., Boone B.A., Bootman M.D., Bordi M., Borner C., Bornhauser B.C., Borthakur G., Bosch J., Bose S., Botana L.M., Botas J., Boulanger C.M., Boulton M.E., Bourdenx M., Bourgeois B., Bourke N.M., Bousquet G., Boya P., Bozhkov P.V., Bozi L.H.M., Bozkurt T.O., Brackney D.E., Brandts C.H., Braun R.J., Braus G.H., Bravo-Sagua R., Bravo-San Pedro J.M., Brest P., Bringer M.-A., Briones-Herrera A., Broaddus V.C., Brodersen P., Brodsky J.L., Brody S.L., Bronson P.G., Bronstein J.M., Brown C.N., Brown R.E., Brum P.C., Brumell J.H., Brunetti-Pierri N., Bruno D., Bryson-Richardson R.J., Bucci C., Buchrieser C., Bueno M., Buitrago-Molina L.E., Buraschi S., Buch S., Buchan J.R., Buckingham E.M., Budak H., Budini M., Bultynck G., Burada F., Burgoyne J.R., Buron M.I., Bustos V., Buttner S., Butturini E., Byrd A., Cabas I., Cabrera-Benitez S., Cadwell K., Cai J., Cai L., Cai 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[Ovid has truncated this field to avoid Excel character limitations], Klionsky D.J., Abdel-Aziz A.K., Abdelfatah S., Abdellatif M., Abdoli A., Abel S., Abeliovich H., Abildgaard M.H., Abudu Y.P., Acevedo-Arozena A., Adamopoulos I.E., Adeli K., Adolph T.E., Adornetto A., Aflaki E., Agam G., Agarwal A., Aggarwal B.B., Agnello M., Agostinis P., Agrewala J.N., Agrotis A., Aguilar P.V., Ahmad S.T., Ahmed Z.M., Ahumada-Castro U., Aits S., Aizawa S., Akkoc Y., Akoumianaki T., Akpinar H.A., Al-Abd A.M., Al-Akra L., Al-Gharaibeh A., Alaoui-Jamali M.A., Alberti S., Alcocer-Gomez E., Alessandri C., Ali M., Alim Al-Bari M.A., Aliwaini S., Alizadeh J., Almacellas E., Almasan A., Alonso A., Alonso G.D., Altan-Bonnet N., Altieri D.C., Alvarez E.M.C., Alves S., Alves da Costa C., Alzaharna M.M., Amadio M., Amantini C., Amaral C., Ambrosio S., Amer A.O., Ammanathan V., An Z., Andersen S.U., Andrabi S.A., Andrade-Silva M., Andres A.M., Angelini S., Ann D., Anozie U.C., Ansari M.Y., Antas P., Antebi A., 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X., Qi Y., Qian W., Qiang L., Qiu Y., Quarleri J., Raben N., Rabinowich H., Ragona D., Ragusa M.J., Rahimi N., Rahmati M., Raia V., Raimundo N., Rajasekaran N.-S., Ramachandra Rao S., Rami A., Ramirez-Pardo I., Ramsden D.B., Randow F., Rangarajan P.N., Ranieri D., Rao H., Rao L., Rao R., Rathore S., Ratnayaka J.A., Ratovitski E.A., Ravanan P., Ravegnini G., Ray S.K., Razani B., Rebecca V., Reggiori F., Regnier-Vigouroux A., Reichert A.S., Reigada D., Reiling J.H., Rein T., Reipert S., Rekha R.S., Ren H., Ren J., Ren W., Renault T., Renga G., Reue K., Rewitz K., Ribeiro de Andrade Ramos B., Riazuddin S.A., Ribeiro-Rodrigues T.M., Ricci J.-E., Ricci R., Riccio V., Richardson D.R., Rikihisa Y., Risbud M.V., Risueno R.M., Ritis K., Rizza S., Rizzuto R., Roberts H.C., Roberts L.D., Robinson K.J., Roccheri M.C., Rocchi S., Rodney G.G., Rodrigues T., Rodrigues Silva V.R., Rodriguez A., Rodriguez-Barrueco R., Rodriguez-Henche N., Rodriguez-Rocha H., Roelofs J., Rogers R.S., Rogov V.V., Rojo 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Viret C., Viscomi M.T., Visnjic D., Vitale I., Vocadlo D.J., Voitsekhovskaja O.V., Volonte C., Volta M., Vomero M., Von Haefen C., Vooijs M.A., Voos W., Vucicevic L., Wade-Martins R., Waguri S., Waite K.A., and Wakatsuki S.
- Abstract
In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field.Copyright © 2020 Informa UK Limited, trading as Taylor & Francis Group.
- Published
- 2021
14. Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)1.
- Author
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Klionsky D.J., Abdel-Aziz A.K., Abdelfatah S., Abdellatif M., Abdoli A., Abel S., Abeliovich H., Abildgaard M.H., Abudu Y.P., Acevedo-Arozena A., Adamopoulos I.E., Adeli K., Adolph T.E., Adornetto A., Aflaki E., Agam G., Agarwal A., Aggarwal B.B., Agnello M., Agostinis P., Agrewala J.N., Agrotis A., Aguilar P.V., Ahmad S.T., Ahmed Z.M., Ahumada-Castro U., Aits S., Aizawa S., Akkoc Y., Akoumianaki T., Akpinar H.A., Al-Abd A.M., Al-Akra L., Al-Gharaibeh A., Alaoui-Jamali M.A., Alberti S., Alcocer-Gomez E., Alessandri C., Ali M., Alim Al-Bari M.A., Aliwaini S., Alizadeh J., Almacellas E., Almasan A., Alonso A., Alonso G.D., Altan-Bonnet N., Altieri D.C., Alvarez E.M.C., Alves S., Alves da Costa C., Alzaharna M.M., Amadio M., Amantini C., Amaral C., Ambrosio S., Amer A.O., Ammanathan V., An Z., Andersen S.U., Andrabi S.A., Andrade-Silva M., Andres A.M., Angelini S., Ann D., Anozie U.C., Ansari M.Y., Antas P., Antebi A., Anton Z., Anwar T., Apetoh L., Apostolova N., Araki T., Araki Y., 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Rogov V.V., Rojo A.I., Rolka K., Romanello V., Romani L., Romano A., Romano P.S., Romeo-Guitart D., Romero L.C., Romero M., Roney J.C., Rongo C., Roperto S., Rosenfeldt M.T., Rosenstiel P., Rosenwald A.G., Roth K.A., Roth L., Roth S., Rouschop K.M.A., Roussel B.D., Roux S., Rovere-Querini P., Roy A., Rozieres A., Ruano D., Rubinsztein D.C., Rubtsova M.P., Ruckdeschel K., Ruckenstuhl C., Rudolf E., Rudolf R., Ruggieri A., Ruparelia A.A., Rusmini P., Russell R.R., Russo G.L., Russo M., Russo R., Ryabaya O.O., Ryan K.M., Ryu K.-Y., Sabater-Arcis M., Sachdev U., Sacher M., Sachse C., Sadhu A., Sadoshima J., Safren N., Saftig P., Sagona A.P., Sahay G., Sahebkar A., Sahin M., Sahin O., Sahni S., Saito N., Saito S., Saito T., Sakai R., Sakai Y., Sakamaki J.-I., Saksela K., Salazar G., Salazar-Degracia A., Salekdeh G.H., Saluja A.K., Sampaio-Marques B., Sanchez M.C., Sanchez-Alcazar J.A., Sanchez-Vera V., Sancho-Shimizu V., Sanderson J.T., Sandri M., Santaguida S., Santambrogio L., Santana 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[Ovid has truncated this field to avoid Excel character limitations]
- Abstract
In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field.Copyright © 2020 Informa UK Limited, trading as Taylor & Francis Group.
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- 2021
15. Tumor antigens heterogeneity and immune response-targeting neoantigens in breast cancer
- Author
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Benvenuto, M. (Monica), Focaccetti, C. (Chiara), Izzi, V. (Valerio), Masuelli, L. (Laura), Modesti, A. (Andrea), Bei, R. (Roberto), Benvenuto, M. (Monica), Focaccetti, C. (Chiara), Izzi, V. (Valerio), Masuelli, L. (Laura), Modesti, A. (Andrea), and Bei, R. (Roberto)
- Abstract
Breast cancer is both the most common type of cancer and the most frequent cause of cancer mortality in women, mainly because of its heterogeneity and limited immunogenicity. The aim of specific active cancer immunotherapy is to stimulate the host‘s immune response against cancer cells directly using a vaccine platform carrying one or more tumor antigens. In particular, the ideal tumor antigen should be able to elicit T cell and B cell responses, be specific for the tumor and be expressed at high levels on cancer cells. Neoantigens are ideal targets for immunotherapy because they are exclusive to individual patient‘s tumors, are absent in healthy tissues and are not subject to immune tolerance mechanisms. Thus, neoantigens should generate a specific reaction towards tumors since they constitute the largest fraction of targets of tumor-infiltrating T cells. In this review, we describe the technologies used for neoantigen discovery, the heterogeneity of neoantigens in breast cancer and recent studies of breast cancer immunotherapy targeting neoantigens.
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- 2021
16. Nutlin-3a enhances natural killer cell-mediated killing of neuroblastoma by restoring p53-dependent expression of ligands for NKG2D and DNAM-1 receptors
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Veneziani, I., Infante, P., Ferretti, E., Melaiu, O., Battistelli, C., Lucarini, V., Compagnone, M., Nicoletti, C., Castellano, A., Petrini, S., Ognibene, M., Pezzolo, A., Di Marcotullio, L., Bei, R., Moretta, L., Pistoia, V., Fruci, D., Barnaba, V., Locatelli, Franco, Cifaldi, L., Locatelli F. (ORCID:0000-0002-7976-3654), Veneziani, I., Infante, P., Ferretti, E., Melaiu, O., Battistelli, C., Lucarini, V., Compagnone, M., Nicoletti, C., Castellano, A., Petrini, S., Ognibene, M., Pezzolo, A., Di Marcotullio, L., Bei, R., Moretta, L., Pistoia, V., Fruci, D., Barnaba, V., Locatelli, Franco, Cifaldi, L., and Locatelli F. (ORCID:0000-0002-7976-3654)
- Abstract
In this study, we explored whether Nutlin-3a, a well-known, nontoxic small-molecule compound antagonizing the inhibitory interaction of MDM2 with the tumor suppressor p53, may restore ligands for natural killer (NK) cell-activating receptors (NK-AR) on neuroblastoma cells to enhance the NK cell-mediated killing. Neuroblastoma cell lines were treated with Nutlin-3a, and the expression of ligands for NKG2D and DNAM-1 NK-ARs and the neuroblastoma susceptibility to NK cells were evaluated. Adoptive transfer of human NK cells in a xenograft neuroblastoma-bearing NSG murine model was assessed. Two data sets of neuroblastoma patients were explored to correlate p53 expression with ligand expression. Luciferase assays and chromatin immunoprecipitation analysis of p53 functional binding on PVR promoter were performed. Primary neuroblastoma cells were also treated with Nutlin-3a, and neuroblastoma spheroids obtained from one high-risk patient were assayed for NK-cell cytotoxicity. We provide evidence showing that the Nutlin-3a-dependent rescue of p53 function in neuroblastoma cells resulted in (i) increased surface expression of ligands for NK-ARs, thus rendering neuroblastoma cell lines significantly more susceptible to NK cell-mediated killing; (ii) shrinkage of human neuroblastoma tumor masses that correlated with overall survival upon adoptive transfer of NK cells in neuroblastoma-bearing mice; (iii) and increased expression of ligands in primary neuroblastoma cells and boosting of NK cell-mediated disaggregation of neuroblastoma spheroids. We also found that p53 was a direct transcription factor regulating the expression of PVR ligand recognized by DNAM-1. Our findings demonstrated an immunomodulatory role of Nutlin-3a, which might be prospectively used for a novel NK cell-based immunotherapy for neuroblastoma.
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- 2021
17. Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)
- Author
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Werner, Brand-Saberi, Beate, Dong, X Charlie, Kenchappa, Chandra Shekar, Li, Zuguo, Lin, Yong, Oshima, Shigeru, Rong, Yueguang, Sluimer, Judith C, Stallings, Christina L, and Tong, Chun-Kit
- Abstract
In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field.
- Published
- 2021
18. Automated estimation of leaf area index from grapevine canopies using cover photography, video and computational analysis methods
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Fuentes, S., Poblete-Echeverría, C., Ortega-Farias, S., Tyerman, S., and De Bei, R.
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- 2014
- Full Text
- View/download PDF
19. Cancer stem cells from peritumoral tissue of glioblastoma multiforme: The possible missing link between tumor development and progression
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Angelucci, C, D'Alessio, A, Lama, G, Binda, E, Mangiola, A, Vescovi, A, Proietti, G, Masuelli, L, Bei, R, Fazi, B, Ciafre, S, Sica, G, Angelucci C., D'Alessio A., Lama G., Binda E., Mangiola A., Vescovi A., Proietti G., Masuelli L., Bei R., Fazi B., Ciafre S. A., Sica G., Angelucci, C, D'Alessio, A, Lama, G, Binda, E, Mangiola, A, Vescovi, A, Proietti, G, Masuelli, L, Bei, R, Fazi, B, Ciafre, S, Sica, G, Angelucci C., D'Alessio A., Lama G., Binda E., Mangiola A., Vescovi A., Proietti G., Masuelli L., Bei R., Fazi B., Ciafre S. A., and Sica G.
- Abstract
In glioblastoma multiforme (GBM), cancer stem cells (CSCs) are thought to be responsible for gliomagenesis, resistance to treatment and recurrence. Unfortunately, the prognosis for GBM remains poor and recurrence frequently occurs in the peritumoral tissue within 2 cm from the tumor edge. In this area, a population of CSCs has been demonstrated which may recapitulate the tumor after surgical resection. In the present study, we aimed to characterize CSCs derived from both peritumoral tissue (PCSCs) and GBM (GCSCs) in order to deepen their significance in GBM development and progression. The stemness of PCSC/GCSC pairs obtained from four human GBM surgical specimens was investigated by comparing the expression of specific stem cell markers such as Nestin, Musashi-1 and SOX2. In addition, the growth rate, the ultrastructural features and the expression of other molecules such as c-Met, pMet and MAP kinases, involved in cell migration/invasion, maintenance of tumor stemness and/or resistance to treatments were evaluated. Since it has been recently demonstrated the involvement of the long noncoding RNAs (lncRNAs) in the progression of gliomas, the expression of H19 lncRNA, as well as of one of its two mature products miR-675-5p was evaluated in neurospheres. Our results show significant differences between GCSCs and PCSCs in terms of proliferation, ultrastructural peculiarities and, at a lower extent, stemness profile. These differences might be important in view of their potential role as a therapeutic target.
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- 2018
20. Effects of canopy management practices on grapevine bud fruitfulness
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Wang, X, Lesefko, S, De Bei, R, Fuentes, S, Collins, C, Wang, X, Lesefko, S, De Bei, R, Fuentes, S, and Collins, C
- Abstract
Background and aims: Bud fruitfulness is a key component of grapevine reproductive performance as it determines crop production for the following growing season. While canopy microclimate can impact bud fruitfulness, the effects of canopy management practices on bud fruitfulness are not well known. The objective of this study was to investigate the effects of common canopy management practices on bud fruitfulness and the relationships with shoot growth capacity, bud microclimate and bud carbohydrate level.Methods and results: Different canopy management practices, (shoot thinning, bunch thinning, leaf removal and lighter pruning) were applied to Semillon and Shiraz grapevines (Vitis vinifera L.). Light interception at the bud zone was measured after canopy management practices were applied. Bud fruitfulness at dormancy was assessed using bud dissection analysis. The number and size of inflorescence primordia, and the incidence of primary bud necrosis were recorded. The results were correlated with measurements of shoot growth capacity and carbohydrate content of buds and canes.Conclusions: Bud fruitfulness was mostly influenced by bud light interception, while the size of inflorescence primordia was positively correlated with shoot growth capacity and the carbohydrate level of buds. By altering canopy microclimate, canopy management practices can be used to manipulate bud fruitfulness and potentially bunch size.Significance and impact of the study: This study provides novel information on the impact of canopy management on grapevine bud fruitfulness and the size of inflorescence primordia. These findings can be used to make more informed vineyard management decisions for better yield control.
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- 2020
21. Shoot thinning of Semillon in a hot climate did not improve yield and berry and wine quality
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De Bei, R, Wang, X, Papagiannis, L, Fuentes, S, Gilliham, M, Tyerman, S, Collins, C, De Bei, R, Wang, X, Papagiannis, L, Fuentes, S, Gilliham, M, Tyerman, S, and Collins, C
- Abstract
Aim: Shoot thinning is a common canopy management practice used to obtain a desired shoot density and to improve canopy microclimate. Since thinning is often carried out manually, the cost can be high. In this study the effect of severe shoot thinning (50 % of shoots removed) applied at EL 15 was investigated by comparing yield components, canopy size, berry and wine chemistry, and sensory attributes to a non-thinned control for the variety Semillon. The objective was to determine whether shoot thinning could change canopy architecture and lead to improved fruit and wine chemistry and sensory characteristics. Methods and results: The trial was carried out over four consecutive growing seasons (starting in 2014-15) in the Semillon block of the Coombe vineyard (Waite Campus, the University of Adelaide). Canopy architecture was monitored at key phenological stages in each season and yield components were assessed at harvest. The harvested fruit was used for chemical and sensory analysis of the berries. Wines were made and their chemistry and sensorial attributes assessed. Shoot thinning reduced the total leaf area in only two of the four seasons, but single shoot leaf area and cane weight were higher in shoot-thinned vines in all seasons. Shoot thinning did not reduce yield, despite a large reduction in bunch number, because of increased bunch weight. Shoot thinning did not change berry and wine chemistry. Similarly, little differences were observed in the sensory profile of berries and wines, and the assessors preferred the wines obtained from shoot thinned vines in the last season only. Conclusions: In this study, shoot thinning increased the leaf area per shoot and the cane weight, but yield and grape and wine chemistry were unaffected. The vine balance indices leaf area/yield and yield/pruning weight were also unaffected by the treatment, despite its intensity (50 % of shoots removed). Significance and impact of the study: The practice of shoot thinning when applie
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- 2020
22. Classification of Smoke Contaminated Cabernet Sauvignon Berries and Leaves Based on Chemical Fingerprinting and Machine Learning Algorithms
- Author
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Summerson, V, Gonzalez Viejo, C, Szeto, C, Wilkinson, KL, Torrico, DD, Pang, A, De Bei, R, Fuentes, S, Summerson, V, Gonzalez Viejo, C, Szeto, C, Wilkinson, KL, Torrico, DD, Pang, A, De Bei, R, and Fuentes, S
- Abstract
Wildfires are an increasing problem worldwide, with their number and intensity predicted to rise due to climate change. When fires occur close to vineyards, this can result in grapevine smoke contamination and, subsequently, the development of smoke taint in wine. Currently, there are no in-field detection systems that growers can use to assess whether their grapevines have been contaminated by smoke. This study evaluated the use of near-infrared (NIR) spectroscopy as a chemical fingerprinting tool, coupled with machine learning, to create a rapid, non-destructive in-field detection system for assessing grapevine smoke contamination. Two artificial neural network models were developed using grapevine leaf spectra (Model 1) and grape spectra (Model 2) as inputs, and smoke treatments as targets. Both models displayed high overall accuracies in classifying the spectral readings according to the smoking treatments (Model 1: 98.00%; Model 2: 97.40%). Ultraviolet to visible spectroscopy was also used to assess the physiological performance and senescence of leaves, and the degree of ripening and anthocyanin content of grapes. The results showed that chemical fingerprinting and machine learning might offer a rapid, in-field detection system for grapevine smoke contamination that will enable growers to make timely decisions following a bushfire event, e.g., avoiding harvest of heavily contaminated grapes for winemaking or assisting with a sample collection of grapes for chemical analysis of smoke taint markers.
- Published
- 2020
23. Targeting cytokine/chemokine receptors: a challenge for molecular nuclear medicine
- Author
-
Signore, A., Chianelli, M., Bei, R., Oyen, W., and Modesti, A.
- Published
- 2003
- Full Text
- View/download PDF
24. Recycling of TV Glass: Profits or Doom?
- Author
-
Hermans, J. M., primary, Peelen, J. G. J., additional, and Bei, R., additional
- Published
- 2008
- Full Text
- View/download PDF
25. Polyphenols as immunomodulatory compounds in the tumor microenvironment:friends or foes?
- Author
-
Focaccetti, C. (Chiara), Izzi, V. (Valerio), Benvenuto, M. (Monica), Fazi, S. (Sara), Ciuffa, S. (Sara), Giganti, M. G. (Maria Gabriella), Potenza, V. (Vito), Manzari, V. (Vittorio), Modesti, A. (Andrea), and Bei, R. (Roberto)
- Subjects
inflammation ,food and beverages ,cancer ,immune response ,polyphenols - Abstract
Polyphenols are natural antioxidant compounds ubiquitously found in plants and, thus, ever present in human nutrition (tea, wine, chocolate, fruits and vegetables are typical examples of polyphenol-rich foods). Widespread evidence indicate that polyphenols exert strong antioxidant, anti-inflammatory, anti-microbial and anti-cancer activities, and thus, they are generally regarded to as all-purpose beneficial nutraceuticals or supplements whose use can only have a positive influence on the body. A closer look to the large body of results of years of investigations, however, present a more complex scenario where polyphenols exert different and, sometimes, paradoxical effects depending on dose, target system and cell type and the biological status of the target cell. Particularly, the immunomodulatory potential of polyphenols presents two opposite faces to researchers trying to evaluate their usability in future cancer therapies: on one hand, these compounds could be beneficial suppressors of peri-tumoral inflammation that fuels cancer growth. On the other hand, they might suppress immunotherapeutic approaches and give rise to immunosuppressive cell clones that, in turn, would aid tumor growth and dissemination. In this review, we summarize knowledge of the immunomodulatory effects of polyphenols with a particular focus on cancer microenvironment and immunotherapy, highlighting conceptual pitfalls and delicate cell-specific effects in order to aid the design of future therapies involving polyphenols as chemoadjuvants.
- Published
- 2019
26. Non-Invasive Tools to Detect Smoke Contamination in Grapevine Canopies, Berries and Wine: A Remote Sensing and Machine Learning Modeling Approach
- Author
-
Fuentes, S, Tongson, EJ, De Bei, R, Viejo, CG, Ristic, R, Tyerman, S, Wilkinson, K, Fuentes, S, Tongson, EJ, De Bei, R, Viejo, CG, Ristic, R, Tyerman, S, and Wilkinson, K
- Abstract
Bushfires are becoming more frequent and intensive due to changing climate. Those that occur close to vineyards can cause smoke contamination of grapevines and grapes, which can affect wines, producing smoke-taint. At present, there are no available practical in-field tools available for detection of smoke contamination or taint in berries. This research proposes a non-invasive/in-field detection system for smoke contamination in grapevine canopies based on predictable changes in stomatal conductance patterns based on infrared thermal image analysis and machine learning modeling based on pattern recognition. A second model was also proposed to quantify levels of smoke-taint related compounds as targets in berries and wines using near-infrared spectroscopy (NIR) as inputs for machine learning fitting modeling. Results showed that the pattern recognition model to detect smoke contamination from canopies had 96% accuracy. The second model to predict smoke taint compounds in berries and wine fit the NIR data with a correlation coefficient (R) of 0.97 and with no indication of overfitting. These methods can offer grape growers quick, affordable, accurate, non-destructive in-field screening tools to assist in vineyard management practices to minimize smoke taint in wines with in-field applications using smartphones and unmanned aerial systems (UAS).
- Published
- 2019
27. Tendon’s ultrastructure
- Author
-
Tresoldi, I., primary, Oliva, F., additional, Benvenuto, M., additional, Fantin, M., additional, Masuelli, L., additional, Bei, R., additional, and Modesti, A., additional
- Published
- 2019
- Full Text
- View/download PDF
28. Cancer stem cells from peritumoral tissue of glioblastoma multiforme: the possible missing link between tumor development and progression.
- Author
-
Angelucci, Cristiana, D'Alessio, Alessio, Lama, Gina, Binda, E, Mangiola, Annunziato, Vescovi, Al, Proietti, G, Masuelli, L, Bei, R, Fazi, Barbara, Ciafrè, Sa, Sica, Gigliola, Angelucci C (ORCID:0000-0002-5177-6533), D'Alessio A (ORCID:0000-0002-3340-2634), Lama G (ORCID:0000-0001-6036-3071), Mangiola A (ORCID:0000-0002-1378-4524), Fazi B, Sica G. (ORCID:0000-0002-7231-9139), Angelucci, Cristiana, D'Alessio, Alessio, Lama, Gina, Binda, E, Mangiola, Annunziato, Vescovi, Al, Proietti, G, Masuelli, L, Bei, R, Fazi, Barbara, Ciafrè, Sa, Sica, Gigliola, Angelucci C (ORCID:0000-0002-5177-6533), D'Alessio A (ORCID:0000-0002-3340-2634), Lama G (ORCID:0000-0001-6036-3071), Mangiola A (ORCID:0000-0002-1378-4524), Fazi B, and Sica G. (ORCID:0000-0002-7231-9139)
- Abstract
In glioblastoma multiforme (GBM), cancer stem cells (CSCs) are thought to be responsible for gliomagenesis, resistance to treatment and recurrence. Unfortunately, the prognosis for GBM remains poor and recurrence frequently occurs in the peritumoral tissue within 2 cm from the tumor edge. In this area, a population of CSCs has been demonstrated which may recapitulate the tumor after surgical resection. In the present study, we aimed to characterize CSCs derived from both peritumoral tissue (PCSCs) and GBM (GCSCs) in order to deepen their significance in GBM development and progression. The stemness of PCSC/GCSC pairs obtained from four human GBM surgical specimens was investigated by comparing the expression of specific stem cell markers such as Nestin, Musashi-1 and SOX2. In addition, the growth rate, the ultrastructural features and the expression of other molecules such as c-Met, pMet and MAP kinases, involved in cell migration/invasion, maintenance of tumor stemness and/or resistance to treatments were evaluated. Since it has been recently demonstrated the involvement of the long non-coding RNAs (lncRNAs) in the progression of gliomas, the expression of H19 lncRNA, as well as of one of its two mature products miR-675-5p was evaluated in neurospheres. Our results show significant differences between GCSCs and PCSCs in terms of proliferation, ultrastructural peculiarities and, at a lower extent, stemness profile. These differences might be important in view of their potential role as a therapeutic target.
- Published
- 2018
29. Feasibility study on the use of Near Infrared spectroscopy to measure water status of almond trees
- Author
-
De Bei, R., primary, Fuentes, S., additional, Wirthensohn, M.G., additional, Cozzolino, D., additional, and Tyerman, S.D., additional
- Published
- 2018
- Full Text
- View/download PDF
30. Effect of vine age on vine performance of Shiraz in the Barossa Valley, Australia
- Author
-
Grigg, D., primary, Methven, D., additional, de Bei, R., additional, Rodríguez López, C.M., additional, Dry, P., additional, and Collins, C., additional
- Published
- 2017
- Full Text
- View/download PDF
31. Chloroquine supplementation increases the cytotoxic effect of curcumin against Her2/neu overexpressing breast cancer cells in vitro and in vivo in nude mice while counteracts it in immune competent mice
- Author
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Masuelli, L., primary, Granato, M., additional, Benvenuto, M., additional, Mattera, R., additional, Bernardini, R., additional, Mattei, M., additional, d'Amati, G., additional, D'Orazi, G., additional, Faggioni, A., additional, Bei, R., additional, and Cirone, M., additional
- Published
- 2017
- Full Text
- View/download PDF
32. Undifferentiated metastatic renal cell carcinoma presenting as a cutaneous nodular lesion|Bir kütanöz nodüler lezyon olarak ortaya çıkan farklılaşmamış metastatik renal hücreli karsinom
- Author
-
Paolino, G, Lido, P, Bei, R, and Polisca, P
- Subjects
Settore MED/04 - Patologia Generale ,renal cell carcinoma ,undifferentiated tumor ,immunohistochemistry ,Cutaneous metastasis - Published
- 2015
33. Mitochondrial control of Caenorhabditis elegans aging via hypoxia-like induction of autophagy
- Author
-
Schiavi A, Torgovnick A, Kell A, Megalou E. V, Castelain N, Guccini I, Marzocchetta L, Gelino S, Hansen M, Malisan F, Condo I, Bei R, Rea S. L, Braeckman B, Tavernarakis N, Testi R. and Ventura N., Schiavi A., Torgovnick A., Kell A., Megalou E. V., Castelain N., Guccini I., Marzocchetta L., Gelino S., Hansen M., Malisan F., Condo I., Bei R., Rea S. L., Braeckman B., Tavernarakis N., Testi R., and Ventura N.
- Published
- 2012
34. VitiCanopy: A Free Computer App to Estimate Canopy Vigor and Porosity for Grapevine
- Author
-
De Bei, R, Fuentes, S, Gilliham, M, Tyerman, S, Edwards, E, Bianchini, N, Smith, J, Collins, C, De Bei, R, Fuentes, S, Gilliham, M, Tyerman, S, Edwards, E, Bianchini, N, Smith, J, and Collins, C
- Abstract
Leaf area index (LAI) and plant area index (PAI) are common and important biophysical parameters used to estimate agronomical variables such as canopy growth, light interception and water requirements of plants and trees. LAI can be either measured directly using destructive methods or indirectly using dedicated and expensive instrumentation, both of which require a high level of know-how to operate equipment, handle data and interpret results. Recently, a novel smartphone and tablet PC application, VitiCanopy, has been developed by a group of researchers from the University of Adelaide and the University of Melbourne, to estimate grapevine canopy size (LAI and PAI), canopy porosity, canopy cover and clumping index. VitiCanopy uses the front in-built camera and GPS capabilities of smartphones and tablet PCs to automatically implement image analysis algorithms on upward-looking digital images of canopies and calculates relevant canopy architecture parameters. Results from the use of VitiCanopy on grapevines correlated well with traditional methods to measure/estimate LAI and PAI. Like other indirect methods, VitiCanopy does not distinguish between leaf and non-leaf material but it was demonstrated that the non-leaf material could be extracted from the results, if needed, to increase accuracy. VitiCanopy is an accurate, user-friendly and free alternative to current techniques used by scientists and viticultural practitioners to assess the dynamics of LAI, PAI and canopy architecture in vineyards, and has the potential to be adapted for use on other plants.
- Published
- 2016
35. Automated estimation of leaf area index from grapevine canopies using cover photography, video and computational analysis
- Author
-
Fuentes, S., Poblete-Echeverria, C., Ortega-Farias, S., Tyerman, S., and De Bei, R.
- Published
- 2014
36. Effects of vitamin C on health: a review of evidence
- Author
-
Grosso, G, Bei, R, Mistretta, Antonio, Marventano, S, Calabrese, G, Masuelli, L, Giganti, Mg, Modesti, A, Galvano, Fabio, Gazzolo, D., and Grosso, Giuseppe
- Subjects
medicine.medical_specialty ,NICOTINAMIDE ADENINE-DINUCLEOTIDE ,Eye Diseases ,SMOOTH-MUSCLE-CELLS ,Critical Illness ,review ,Connective tissue ,Physiology ,Inflammation ,Ascorbic Acid ,Peptide hormone ,Review KeyWords Plus:LOW-DENSITY-LIPOPROTEIN ,Nervous System ,chemistry.chemical_compound ,Author Keywords:Vitamin C ,Cardiovascular Disease ,Cancer ,Anti-Inflammation ,Antioxidant ,OXIDATIVE DNA-DAMAGE ,NITRIC-OXIDE SYNTHASE ,VASCULAR ENDOTHELIAL-CELLS ,CORONARY-ARTERY-DISEASE ,CRITICALLY-ILL PATIENTS ,ASCORBIC-ACID PROTECTS ,GAMMA-LACTONE OXIDASE ,anti-inflammation ,ascorbic acid ,cardiovascular disease ,antioxidant ,vitamin c ,cancer ,Internal medicine ,medicine ,Anticarcinogenic Agents ,Humans ,Settore MED/04 - Patologia Generale ,Vitamin C ,Cholesterol ,business.industry ,Scurvy ,medicine.disease ,medicine.anatomical_structure ,Endocrinology ,chemistry ,Cardiovascular Diseases ,medicine.symptom ,business ,Blood vessel - Abstract
Vitamin C is an essential dietary nutrient for the biosynthesis of collagen and a co-factor in the biosynthesis of catecholamines, L-carnitine, cholesterol, amino acids, and some peptide hormones. The lack of vitamin C causes scurvy, a pathological condition leading to blood vessel fragility and connective tissue damage due to failure in producing collagen, and, finally, to death as result of a general collapse. Vitamin C is potentially involved also in cancer and cardiovascular diseases prevention. In addition, vitamin C effects on nervous system and chronically ill patients have been also documented. This review attempts to summarize recent and well established advances in vitamin C research and its clinical implications. Since vitamin C has the potential to counteract inflammation and subsequent oxidative damage that play a major role in the initiation and progression of several chronic and acute diseases, it represents a practical tool to administer for the early prevention of these pathologic conditions.
- Published
- 2013
37. Assessment of grapevine water status by ground-based hyperspectral imaging
- Author
-
Diago, Maria P., De Bei, R., Millán Prior, Borja, Poni, Stefano, Gatti, M., Bemizzoni, F., Tyerman, S., Tardáguila, Javier, and Viña Alta Alcurnia
- Abstract
Trabajo presentado en el IX International Symposium on Grapevine Physiology and Biotechnology, celebrado en La Serena (Chile) del 21 al 26 de abril de 2013., The wine industry worldwide is under considerable long-term pressure because of climate change, resulting in increased water scarcity and rising operational costs. The Vineyard of The Future (V oF) is an intemational and multidisciplinary initiative to establish fully instrumented vineyards for clirnate change research. One of the strategies proposed is the use of hyperspectral irnaging as a tool to assist in the estirnation of vine water status of grapevines. In th is study, outdoors hyperspectral irnages of the whole canopy were taken from 18 fi ve-year old Sangiovese ( Vitis vinifera L.) grapevines. The vines were grown outside in 70L pots near Piacenza (Italy). Images were taken over four dates during August 2011. Images After the Chilean wine crisis in the 1980s decade, in 1994 the vineyard plantations resumed. These plantations were done under an old technological paradigm, i.e., mass selection and the obtainment of plant material from renowned vineyards, without a prior sanitary and varietal selection-making. These vineyards normal! y present a mixture of cultivars and sorne pathological problerns, such as trunk canker, virus diseases and nematodes. A grapevine cultivar found mixed within other cultivars (including Cabernet-Sauvignon, Merlot and Cót rouge), inside a vineyard cv. Carmem!re at Palmilla (Colchagua Valley, Chile), were identified by classical ampelographic description and by molecular genetic markers (RAPD and AFLP). The agronomic traits of the unidentified and problematic cultivar, which presented an inflorescence necrosis phenomena, were characterized by pheno1ogica1 tracking (in re1ation to cv. Carmenere), detailed description of flowering phenomena, flower microscope slides and assessing the effect of cane girdling on inflorescence necrosis and fruit set., Acknowledgments: "Viña Alta Alcurnia".
- Published
- 2013
38. Infra-Red thermal image analysis for grapevines
- Author
-
Poblete-Echeverría, C., Fuentes, Sigfredo, De Bei, R., Ortega-Farias, S., Diago, Maria P., Tardáguila, Javier, Comisión Nacional de Investigación Científica y Tecnológica (Chile), Universidad de Talca, and European Commission
- Subjects
MATLAB® programming ,Climate change ,Remote sensing ,Image analysis - Abstract
Trabajo presentado en el 18th International Symposium of the Group of International Experts of vitivinicultural Systems for CoOperation (GIESCO 2013), celebrado en Oporto del 7 al 11 de julio de 2013.-- Número fuera de serie., Infrared thermal images (IRTI) have been used for grapevine research since the early 90’s. Even though its promising results in the assessment of canopy stomatal conductance and plant water status, from the beginning and recent research publications, it has not been fully applied on a commercial scale yet. It is believed that the bottleneck for this technology is the lack of reliable automation tools for IRTI analysis. Accurate and reliable automation technique s will allow the use of this technique to assess the spatial variability of physiological processes within the canopy using infrared cameras mounted on moving vehicles, drones, octocopters or robots. Automated analysis systems are requirement of The Vineyard of The Future initiative, which is an international effort to establis h fully monitored vineyards in the most prominent viticultural and winemaking areas in the world. In this work, a semi-automated IRTI analyses performed using a code written in MATLAB® for estimate dry and wet references excluding non-leaf temperatures was compared with evaporimeter (EvapoSensor, Skye Instruments Ltd, Powys, UK) measurements used to provide dry and wet references from IRTIs. Results obtained from this research (grapevines cv. Tempranillo) showed good and statistically significant correlations between temperatur e references obtained from IRTI analysis and measured values. This work constitutes one additional step forward to the implementation of thermal imaging as an automated routine technique for physiological vineyard assess ment from proximal sensing and unmanned aerial vehicles (UAV) platforms., The research leading to this report was supported by the Spanish project “STRESSIMAGING HPRN-CT-2002-00254” and Chilean projects CONICYT (Nº 79090035) and Programa de Investigación sobre Adaptación de la Agricultura al Cambio Climático - PIEI (Universidad de Talca).
- Published
- 2013
39. Curcumin induces apoptosis in breast cancer cell lines and delays the growth of mammary tumors in neu transgenic mice
- Author
-
Masuelli, L., Monica Benvenuto, Fantini, M., Marzocchella, L., Sacchetti, P., Di Stefano, E., Tresoldi, I., Izzi, V., Bernardini, R., Palumbo, C., Mattei, M., Lista, F., Galvano, F., Modesti, A., and Bei, R.
- Subjects
Curcumin ,Receptor, ErbB-2 ,Cell Survival ,MAP Kinase Signaling System ,Mammary Neoplasms, Animal ,Mice, Transgenic ,Apoptosis ,Breast Neoplasms ,Drug Screening Assays ,Transgenic ,Cell Line ,Mice ,Cell Line, Tumor ,Animals ,Humans ,Phosphorylation ,Inbred BALB C ,erbB-2 ,bcl-2-Associated X Protein ,Cell Proliferation ,Settore MED/04 - Patologia Generale ,Mice, Inbred BALB C ,Tumor ,Animal ,Mammary Neoplasms ,Cell Cycle ,Antitumor ,Fibroblasts ,Rats ,NIH 3T3 Cells ,Female ,Drug Screening Assays, Antitumor ,Poly(ADP-ribose) Polymerases ,Receptor, erbB-2 ,Receptor - Abstract
Breast cancer is a leading cancer in women and despite the benefits of the current therapies a significant number of patients with this tumor is at risk of relapse. Some of the alterations taking place in breast cancer cells are currently exploited by molecularly targeted drugs. Different drugs have been developed which target a single molecule but, given that the tumor originates from the dysregulation of many genes, there is the need to find new drugs that have more than one molecular target. Curcumin [1,7-bis-(4-hydroxy-3-methoxyphenyl)-1,6-heptadiene-3,5-dione] (CUR), a polyphenolic compound found in the spice turmeric, is a pleiotropic molecule able to interact with a variety of molecular targets and has antitumor, anti-inflammatory, antioxidant, immunomodulatory and antimicrobial activities. Here we demonstrate that CUR inhibits the growth of breast cancer cell lines in a dose dependent manner, with IC50 values in the micromolar range, and induces an increase in the percentage of cells in sub-G0 phase, representing the apoptotic cell population. The activation of apoptosis was confirmed by PARP-1 cleavage and by the increased ratio between the pro-apoptotic Bax and the anti-apoptotic Bcl-2 protein. In addition, in CUR-treated cells the activity of ERK1/ERK2 MAP kinases was down-regulated. The cytotoxic effects of CUR were observed in breast cancer cells expressing either high or low levels of ErbB2/neu. The in vivo antitumor activity of CUR was tested in BALB-neuT mice transgenic for the neu oncogene, which develop atypical hyperplasia of the mammary gland at 6 weeks of age and invasive carcinoma at 16 weeks of age. CUR, administered to mice both early and in an advanced stage of mammary carcinogenesis, induced a significant prolongation of tumor-free survival and a reduction of tumor multiplicity. In addition, CUR administration was safe, since no modification of hematological and clinical chemistry parameters could be observed in BALB-neuT and BALB/c mice treated with this compound for several weeks. These findings support further studies on the therapeutic potential of CUR in combination with standard therapies in breast cancer patients.
- Published
- 2013
40. Changes in serum levels of TNF-α, IL-6, OPG, RANKL and their correlation with radiographic and clinical assessment in fragility fractures and high energy fractures
- Author
-
Giganti, M. G., Liuni, F., Celi, M., Gasbarra, E., rossella zenobi, Tresoldi, I., Modesti, A., Bei, R., and Tarantino, U.
- Subjects
Settore MED/04 - Patologia Generale ,inflammatory cytokines ,RUST ,Settore BIO/12 ,fragility fractures ,inflammatory cytokines, fragility fractures, high energy fractures, RUST, LEM ,high energy fractures ,LEM - Published
- 2012
41. Inadequate Emergency Department Analgesia in the Elderly
- Author
-
Gaeta, TJ, Birkhahn, R, Kliot, B, and Bei, R
- Subjects
Emergency medicine -- Research ,Health - Published
- 2001
42. Cryptic epitopes on alpha-fetoprotein induce spontaneous immune responses in hepatocellular carcinoma, liver cirrhosis, and chronic hepatitis patients
- Author
-
BEI R, BUDILLON A, REALE MG, CAPUANO G, POMPONI D, FRATI L, MURARO R., BUDILLON, GABRIELE, Bei, R, Budillon, A, Reale, Mg, Capuano, Gaetano, Pomponi, D, Budillon, G, Frati, L, Muraro, R., Capuano, G, and Budillon, Gabriele
- Subjects
Adult ,Liver Cirrhosis ,Carcinoma, Hepatocellular ,AUTOIMMUNITY ,Blotting, Western ,Liver Neoplasms ,Middle Aged ,Settore MED/04 ,CANCER ,TUMORS ,Precipitin Tests ,ANTIGENS ,Epitopes ,ANTIBODY ,Immunoglobulin M ,Immunoglobulin G ,Humans ,alpha-Fetoproteins ,Aged ,Hepatitis, Chronic - Abstract
To determine alpha-fetoprotein (AFP) immunogenicity in vivo, the presence of antibodies in sera of 60 hepatocellular carcinoma, 15 liver cirrhosis, and 15 chronic hepatitis patients was evaluated by Western blotting and immunoprecipitation analyses using purified human AFP. High titers of anti-AFP immunoglobulins were detected in 14 hepatocellular carcinomas (P = 0.0006), 3 liver cirrhosis (P = 0.0173), and 1 chronic hepatitis patient, but they were not detected in 40 healthy individuals. Therefore, spontaneous immune responses to AFP are significantly associated to liver diseases (P = 0.0015). Patient immunoglobulins recognized proteic linear epitopes that were cryptic in the native protein, as demonstrated by their restricted reactivity with denatured deglycosylated AFP. Thus, in pathological liver conditions, tolerance to this self-molecule is circumvented. The identification of AFP immunogenic epitopes may contribute to defining novel immunotherapeutic strategies targeting this antigen.
- Published
- 1999
43. Effects of mm-waves on human fibroblasts in-vitro
- Author
-
Gallerano, G. P., primary, Doria, A., additional, Giovenale, E., additional, De Amicis, A., additional, De Sanctis, S., additional, Di Cristofaro, S., additional, Franchini, V., additional, Lista, F., additional, Regalbuto, E., additional, Sgura, A., additional, Coluzzi, E., additional, Marinaccio, J., additional, Bei, R., additional, Fantini, M., additional, Benvenuto, M., additional, and Masuelli, L., additional
- Published
- 2015
- Full Text
- View/download PDF
44. Characterization of ribes nigrum and rosmarinus officinalis food supplements
- Author
-
Grosso, A, Marzocchella, L, Aducci, P, Bei, R, and Marra, M
- Subjects
Settore BIO/04 ,food supplement ,plant secondary metabolites - Published
- 2010
45. L'utilizzo topico del sucralfato nel processo di guarigione delle ferite
- Author
-
Bei, R, Masuelli, Laura, Tumino, Giovanni, and Grosso, E.
- Published
- 2010
46. Enzymes activity inhibition by anthocyanins: role on cancer prevention and progression
- Author
-
Bei, R, Masuelli, L, Turriziani, M, LI VOLTI, Giovanni, Malaguarnera, M, and Galvano, Fabio
- Published
- 2009
47. Autoantibodies to extracellular matrix components as outcome of tissue chronic inflammation
- Author
-
Bei, R, Masuelli, Laura, Palumbo, C, and Modesti, A.
- Published
- 2008
48. Intercalated disks remodeling in δ-sarcoglycan deficient hamsters fed with alpha-linolenic acid-enriched diet
- Author
-
Masuelli, L, Trono, P, Marzocchella, L, Mrozek, Ma, Palumbo, C, Minieri, M, Carotenuto, F, Fiaccavento, R, Nardi, A, Galvano, Fabio, DI NARDO, P, Modesti, A, and Bei, R.
- Published
- 2008
49. Performance of the cultivar Merlot under different training systems
- Author
-
DE BEI, R. and Ponchia, Giorgio
- Published
- 2008
50. Effetto della disposizione della chioma sul comportamento vegeto-produttivo dei vitigni Cabernet sauvignon e Merlot (primi risultati)
- Author
-
DE BEI, R., Sivilotti, P, Pascarella, G, Tomasi, D, and Ponchia, Giorgio
- Published
- 2007
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