2,938 results on '"Bei, Zhang"'
Search Results
2. Single-cell multiomics reveals simvastatin inhibits pan-cancer epithelial-mesenchymal transition via the MEK/ERK pathway in XBP1+ mast cells
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Sen Lin, Huimin Zhang, Ruiqi Zhao, Zhulin Wu, Weiqing Zhang, Mengjiao Yu, Bei Zhang, Lanyue Ma, Danfei Li, Lisheng Peng, and Weijun Luo
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Pan-cancer ,Distant metastasis ,Mast cells ,HMG-CoA reductase inhibitors ,Multi-omics ,Medicine ,Science - Abstract
Abstract Distant metastasis is the leading cause of cancer-related mortality, and achieving survival benefits through advancements in systemic therapy remains challenging. Mast cells play a dual role in shaping the tumor microenvironment (TME) and influencing distant metastasis, underscoring the significant research value of targeting mast cells for systemic therapy in advanced cancer. We investigated variations in mast cell infiltration levels in primary and metastatic malignancies using immunocyte infiltration analysis. Mast cell subsets were identified from pan-cancer distant metastasis single-cell sequencing data through dimensionality reduction clustering and cell type annotation, combined with cell trajectory and communication network analyses. A prognostic model was established using WGCNA and 12 machine learning algorithms to identify potential mast cell targets. Drug sensitivity and Mendelian randomization analyses were conducted to select potential drugs targeting mast cells, and their effects on epithelial-mesenchymal transition (EMT) were validated through in vitro experiments, including wound healing, transwell, and western blot assays. Results revealed that activated mast cells show increased infiltration in metastatic tumors, correlating with poor survival duration. XBP1+ mast cells were identified as key components of the inhibitory TME, potentially involved in EMT activation. Simvastatin was identified as a potential drug, reversing EMT induced by XBP1+ mast cells in pan-cancer. Aberrant activation of MEK/ERK signaling in XBP1+ mast cells can stimulate cancer cell EMT by modulating degranulation, while Simvastatin can inhibit EMT by suppressing degranulation.
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- 2024
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3. Recurrent BMP4 variants in exon 4 cause non-HFE-associated hemochromatosis via the BMP/SMAD signaling pathway
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Qin Ouyang, Yanmeng Li, Anjian Xu, Ning Zhang, Sisi Chen, Donghu Zhou, Bei Zhang, Xiaojuan Ou, Jidong Jia, Jian Huang, and Wei Zhang
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Hemochromatosis ,BMP4 p.H251Y variant ,BMP4 p.R269Q variant ,Hepcidin ,BMP/SMAD ,Medicine - Abstract
Abstract Background Hereditary hemochromatosis (HH) is an iron overload disorder and can be caused by variants in non-HFE genes in Chinese patients. However, there is still a considerable proportion of patients suffering from unexplained iron overload. In our previous study, we had identified the p.R269Q variant in exon 4 of the Bone morphogenetic protein 4 (BMP4) gene in Chinese patients with unexplained primary iron overload by Whole Exome sequencing, and then the BMP4 p.H251Y variant was identified by Sanger sequencing in a Chinese patient with secondary iron overload. Our study aimed to explore the pathogenicity and underlying mechanism of BMP4 p.H251Y and BMP4 p.R269Q variants in patients with iron overload. Methods Sanger sequencing was conducted to identify the novel variants in the BMP4 gene of patients with unexplained iron overload. MRI and liver biopsy were used to display iron overload in the liver of the patient harboring the BMP4 p.H251Y variant. The BMP4 and hepcidin levels in BMP4 knockdown and BMP4 variant cells were examined by enzyme-linked immunosorbent assay. The effects of BMP4 p.H251Y and BMP4 p.R269Q variants on the hepcidin-regulation pathway were studied. Results One of 54 HH patients (1.85%) harbored the BMP4 p.R269Q variant. One of 148 patients (0.68%) with secondary hemochromatosis harbored the BMP4 p.H251Y variant, and these two variants were not found in 100 Chinese general population. For the patient harboring the BMP4 p.H251Y variant, abdominal MRI and Perl's staining of liver tissue displayed iron overload in the liver. Cells transfected with the BMP4 p.H251Y and p.R269Q variants showed down-regulation of hepcidin level and BMP/SMAD pathway compared with cells transfected with the wild-type BMP4 vector. Conclusion The BMP4 p.H251Y and p.R269Q variants can downregulate hepcidin levels by inhibiting the BMP/SMAD axis, suggesting they may play pathogenic roles in iron overload.
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- 2024
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4. Advances and prospects in tumor infiltrating lymphocyte therapy
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Xu Qiu, Shengjun Li, Tianyu Fan, Yue Zhang, Bin Wang, Bei Zhang, Mingzhe Zhang, and Li Zhang
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Tumor infiltrating lymphocyte (TIL) ,T-cells ,Adoptive cell therapy (ACT) ,Immunotherapy ,Tumor treatment ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Tumor-infiltrating lymphocyte (TIL) therapy in adoptive T-cell therapy (ACT) has already caused durable regression in a variety of cancer types due to T-cell persistence, clinical activity, and duration of objective response and safety. TILs are composed of polyclonal effector T-cells specific to heterogenetic tumor antigens, reasonably providing a promising means for tumor therapy. In addition, their expansion in vitro can release them from the suppressive tumor microenvironment. Even though significant advances have been made in the procedure of TIL therapy, from TIL isolation, modification, expansion, and infusion back to the patient to target the tumor, strategy optimization is always ongoing to overcome drawbacks such as a complex process, options for the lineage differentiation status of TILs, and sufficient trafficking of TILs to the tumor. In this review, we summarize the current advances of TIL therapy, raise problem-based optimization strategies, and provide future perspectives on next-generation TIL therapy as a potential avenue for enhancing cell-based immunotherapy.
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- 2024
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5. Curcumin inhibits oxidative stress and autophagy in C17.2 neural stem cell through ERK1/2 signaling pathways
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Yuting Ruan, Haoyu Luo, Jingyi Tang, Mengyao Ji, Dapeng Yu, Qun Yu, Zhiyu Cao, Yingren Mai, Bei Zhang, Yan Chen, Jun Liu, and Wang Liao
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autophagy ,curcumin ,oxidative stress ,Geriatrics ,RC952-954.6 - Abstract
Abstract Objectives This study investigates curcumin's neuroprotective role and its potential in promoting neurogenesis in progenitor cells within the brain. Notably, curcumin's antioxidant properties have been implicated in Alzheimer's disease treatment. However, the association between curcumin's antioxidative effects and its impact on neural stem cells (NSCs) remains to be elucidated. Methods C17.2 neural stem cells were utilized as a model to simulate oxidative stress, induced by hydrogen peroxide (H2O2). We quantified the levels of superoxide dismutase (SOD), malondialdehyde (MDA), and intracellular reactive oxygen species (ROS), alongside the gene expression of SOD1 and SOD2, to assess intracellular oxidative stress. Additionally, Western blot analysis was conducted to measure the expressions of LC3‐II, Beclin‐1, and phosphorylated ERK (p‐ERK), thereby evaluating autophagy and ERK signaling pathway activation. Results Treatment with curcumin resulted in a reduction of MDA and ROS levels, suggesting a protective effect on NSCs against oxidative damage induced by H2O2. Furthermore, a decrease in the relative expressions of LC3‐II, Beclin‐1, and p‐ERK was observed post‐curcumin treatment. Conclusions The findings suggest that curcumin may confer protection against oxidative stress by attenuating autophagy and deactivating the ERK1/2 signaling pathways, which could contribute to therapeutic strategies for Alzheimer's disease.
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- 2024
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6. Engineering dendritic cell biomimetic membrane as a delivery system for tumor targeted therapy
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Huiyang Liu, Yiming Lu, Jinbao Zong, Bei Zhang, Xiaolu Li, Hongzhao Qi, Tao Yu, and Yu Li
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Immunotherapy ,Dendritic cell biomimetic nanoparticles ,Cancer therapy ,Cell membrane coating ,Hybrid cell membrane ,Biotechnology ,TP248.13-248.65 ,Medical technology ,R855-855.5 - Abstract
Abstract Targeted immunotherapies make substantial strides in clinical cancer care due to their ability to counteract the tumor's capacity to suppress immune responses. Advances in biomimetic technology with minimally immunogenic and highly targeted, are addressing issues of targeted drug delivery and disrupting the tumor's immunosuppressive environment to trigger immune activation. Specifically, the use of dendritic cell (DC) membranes to coat nanoparticles ensures targeted delivery due to DC's unique ability to activate naive T cells, spotlighting their role in immunotherapy aimed at disrupting the tumor microenvironment. The potential of DC's biomimetic membrane to mediate immune activation and target tumors is gaining momentum, enhancing the effectiveness of cancer treatments in conjunction with other immune responses. This review delves into the methodologies behind crafting DC membranes and the fusion of dendritic and tumor cell membranes for encapsulating therapeutic nanoparticles. It explores their applications and recent advancements in combating cancer, offering an all-encompassing perspective on DC biomimetic nanosystems, immunotherapy driven by antigen presentation, and the collaborative efforts of drug delivery in chemotherapy and photodynamic therapies. Current evidence shows promise in augmenting combined therapeutic approaches for cancer treatment and holds translational potential for various cancer treatments in a clinical setting. Graphical Abstract
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- 2024
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7. Quantitative brain T1 maps derived from T1-weighted MRI acquisitions: a proof-of-concept study
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Audrey Lavielle, Noël Pinaud, Bei Zhang, and Yannick Crémillieux
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Brain ,Brain neoplasms ,Contrast media ,Magnetic resonance imaging ,T1 mapping ,Medical physics. Medical radiology. Nuclear medicine ,R895-920 - Abstract
Abstract Background Longitudinal T1 relaxation time is a key imaging biomarker. In addition, T1 values are modulated by the administration of T1 contrast agents used in patients with tumors and metastases. However, in clinical practice, dedicated T1 mapping sequences are often not included in brain MRI protocols. The aim of this study is to address the absence of dedicated T1 mapping sequences in imaging protocol by deriving T1 maps from standard T1-weighted sequences. Methods A phantom, composed of 144 solutions of paramagnetic agents at different concentrations, was imaged with a three-dimensional (3D) T1-weighed turbo spin-echo (TSE) sequence designed for brain imaging. The relationship between the T1 values and the signal intensities was established using this phantom acquisition. T1 mapping derived from 3D T1-weighted TSE acquisitions in four healthy volunteers and one patient with brain metastases were established and compared to reference T1 mapping technique. The concentration of Gd-based contrast agents in brain metastases were assessed from the derived T1 maps. Results Based on the phantom acquisition, the relationship between T1 values and signal intensity (SI) was found equal to T1 = 0.35 × SI− 1.11 (R 2 = 0.97). TSE-derived T1 values measured in white matter and gray matter in healthy volunteers were equal to 0.997 ± 0.096 s and 1.358 ± 0.056 s (mean ± standard deviation), respectively. Mean Gd3+ concentration value in brain metastases was 94.7 ± 30.0 μM. Conclusion The in vivo results support the relevance of the phantom-based approach: brain T1 maps can be derived from T1-weighted acquisitions. Relevance statement High-resolution brain T1 maps can be generated, and contrast agent concentration can be quantified and imaged in brain metastases using routine 3D T1-weighted TSE acquisitions. Key Points Quantitative T1 mapping adds significant value to MRI diagnostics. T1 measurement sequences are rarely included in routine protocols. T1 mapping and concentration of contrast agents can be derived from routine standard scans. The diagnostic value of MRI can be improved without additional scan time. Graphical Abstract
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- 2024
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8. Damage power enhancement of fuel air explosive with typical metal hydrides additions
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Bei-bei Zhang, Yang-fan Cheng, Xiao-wen Ma, and Hong-hao Ma
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Fuel air explosive ,Metal hydrides ,Damage effect ,Shock wave ,Energetic additive ,Engineering (General). Civil engineering (General) ,TA1-2040 - Abstract
To study the damage power enhancement of fuel air explosive (FAE) with metal hydrides, the effects of metal hydrides (TiH2, MgH2, ZrH2) powders on shock wave and thermal damage of pure propylene oxide (PO) were explored using a 20 L spherical explosion test system combined with colorimetric thermometry technology. The experimental results showed that compared with the base metal powders, the explosion overpressures, maximum pressure rise rates and maximum average temperatures of the solid-liquid mixed fuel with the metal hydrides (TiH2, MgH2, ZrH2) powders increased by 11.04 %, 22.61 %, 4.80 % and 26.68 %, 38.18 %, 13.91 % as well as 6.85 %, 8.57 %, 1.34 %, respectively. Furthermore, the effects of metal hydride powders on the cloud explosion fuel were better than those of Al powders, and MgH2 powders had the most significant effects on the damage power enhancement of pure PO. Metal hydride powders as high-energy additives could improve the energy release characteristics of FAE.
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- 2024
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9. Experimental study on mechanical behavior of silty-fine sand reinforced by a new type of permeable polymer material under dry-wet cycles
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Yuke Wang, Liao Zhang, Xinming Qu, Mengcheng Liu, Yanhui Zhong, and Bei Zhang
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Silty-fine sand ,Permeable polymer ,Dry-wet cycles ,Strength characteristics ,Engineering geology. Rock mechanics. Soil mechanics. Underground construction ,TA703-712 - Abstract
Silty-fine sand in the Yellow River floodplain is prone to geological hazards such as collapse, subsidence, gushing water, and sand routing triggered by groundwater seepage. As a new type of grouting material, permeable polymer is effective for solving silty-fine sand geologic hazards. With the rise and fall of the water table, capillary water, and transpiration, the reinforced silty-fine sand will be subjected to dry-wet cycles. Investigating the strength loss mechanism of specimens reinforced with permeable polymer during dry-wet cycles is essential for mitigating silty-fine sand geological hazards. The strength characteristics of permeable polymer grout-reinforced silty-fine sand specimens under dry-wet cycles and its extreme case (long-time immersion) were analyzed from macro and micro perspectives employing unconfined compressive strength (UCS) test and scanning electron microscope (SEM). The functional calculation model between the specimens and the initial grouting conditions under dry-wet cycles was constructed, and the main influencing factors of the UCS loss of the specimens were obtained. After permeable polymer grouting, the porosity of silty-fine sand is reduced effectively and the porous structure is changed. The grouted specimens maintain structural integrity and demonstrate excellent water stability even after dry-wet cycles.
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- 2024
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10. Development of machine learning-based personalized predictive models for risk evaluation of hepatocellular carcinoma in hepatitis B virus-related cirrhosis patients with low levels of serum alpha-fetoprotein
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Yuan Xu, Bei Zhang, Fan Zhou, Ying-ping Yi, Xin-Lei Yang, Xiao Ouyang, and Hui Hu
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Hepatocellular carcinoma ,Alpha-fetoprotein ,Machine learning algorithms ,Prediction model ,Specialties of internal medicine ,RC581-951 - Abstract
Introduction and Objectives: The increasing incidence of hepatocellular carcinoma (HCC) in China is an urgent issue, necessitating early diagnosis and treatment. This study aimed to develop personalized predictive models by combining machine learning (ML) technology with a demographic, medical history, and noninvasive biomarker data. These models can enhance the decision-making capabilities of physicians for HCC in hepatitis B virus (HBV)-related cirrhosis patients with low serum alpha-fetoprotein (AFP) levels. Patients and Methods: A total of 6,980 patients treated between January 2012 and December 2018 were included. Pre-treatment laboratory tests and clinical data were obtained. The significant risk factors for HCC were identified, and the relative risk of each variable affecting its diagnosis was calculated using ML and univariate regression analysis. The data set was then randomly partitioned into validation (20 %) and training sets (80 %) to develop the ML models. Results: Twelve independent risk factors for HCC were identified using Gaussian naïve Bayes, extreme gradient boosting (XGBoost), random forest, and least absolute shrinkage and selection operation regression models. Multivariate analysis revealed that male sex, age >60 years, alkaline phosphate >150 U/L, AFP >25 ng/mL, carcinoembryonic antigen >5 ng/mL, and fibrinogen >4 g/L were the risk factors, whereas hypertension, calcium 6.8 μmol/L, hemoglobin 40 U/L were the protective factors in HCC patients. Based on these factors, a nomogram was constructed, showing an area under the curve (AUC) of 0.746 (sensitivity = 0.710, specificity=0.646), which was significantly higher than AFP AUC of 0.658 (sensitivity = 0.462, specificity=0.766). Compared with several ML algorithms, the XGBoost model had an AUC of 0.832 (sensitivity = 0.745, specificity=0.766) and an independent validation AUC of 0.829 (sensitivity = 0.766, specificity = 0.737), making it the top-performing model in both sets. The external validation results have proven the accuracy of the XGBoost model. Conclusions: The proposed XGBoost demonstrated a promising ability for individualized prediction of HCC in HBV-related cirrhosis patients with low-level AFP.
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- 2024
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11. Photoinduced Single Electron Reduction of the 4‐O‐5 Linkage in Lignin Models for C‐P Coupling Catalyzed by Bifunctional N‐Heterocyclic Carbenes
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Qiang Liu, Ying‐Zheng Ren, Bei‐Bei Zhang, Wen‐Xin Tang, Zhi‐Xiang Wang, Lin He, and Xiang‐Yu Chen
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bifunctional N‐heterocyclic carbenes ,C─O bond activation ,lignin ,one electron reduction ,trivalent phosphines ,Science - Abstract
Abstract Catalytic activation of Caryl‐O bonds is considered as a powerful strategy for the production of aromatics from lignin. However, due to the high reduction potentials of diaryl ether 4‐O‐5 linkage models, their single electron reduction remains a daunting challenge. This study presents the blue light‐induced bifunctional N‐heterocyclic carbene (NHC)‐catalyzed one‐electron reduction of diaryl ether 4‐O‐5 linkage models for the synthesis of trivalent phosphines. The H‐bond between the newly devised bifunctional NHC and diaryl ethers is responsible for the success of the single electron transfer. Furthermore, this approach demonstrates selective one‐electron reduction of unsymmetric diaryl ethers, oligomeric phenylene oxide, and lignin model.
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- 2024
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12. Nasopharyngeal carcinoma-associated inflammatory cytokines: ongoing biomarkers
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Chuwen Liang, Jun Kan, Jingli Wang, Wei Lu, Xiaoyan Mo, and Bei Zhang
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nasopharyngeal carcinoma ,inflammation ,cytokines ,predictive factors ,prognostic factors ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Nasopharyngeal carcinoma (NPC) is a neoplasm related to inflammation; the expression of cytokines, such as CCL3, CCL4, CCL20, IL-1α, IL-1β, IL-6, IL-8, and IL-10, among others, is presumed to be associated with NPC occurrence and development. Therefore, the circulating levels of these cytokines may be potential biomarkers for assessing tumor aggressiveness, exploring cellular interactions, and monitoring tumor therapeutic responses. Numerous scholars have comprehensively explored the putative mechanisms through which these inflammatory factors affect NPC progression and therapeutic responses. Moreover, investigations have focused on elucidating the correlation between the systemic levels of these cytokines and the incidence and prognosis of NPC. This comprehensive review aims to delineate the advancements in research concerning the relationship between inflammatory factors and NPC while considering their prospective roles as novel prognostic and predictive biomarkers in the context of NPC.
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- 2024
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13. Elevational patterns of soil organic carbon and its fractions in tropical seasonal rainforests in karst peak-cluster depression region
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Bei Zhang, Chaohao Xu, Zhonghua Zhang, Cong Hu, Chaofang Zhong, Siyu Chen, and Gang Hu
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karst ,soil organic carbon ,recalcitrant organic carbon ,labile organic carbon fractions ,southwest China ,Plant culture ,SB1-1110 - Abstract
Karst ecosystems, especially tropical karst forests, are crucial to the global carbon cycle. In mountainous and hilly areas, elevation-related changes in environment and vegetation often lead to shifts in the accumulation and decomposition of soil organic carbon (SOC). However, the elevational patterns and influencing variables of SOC and its fractions in tropical karst forest ecosystems remain largely unexplored. Here, we analyzed the elevational patterns of SOC and its fractions in the topsoil and subsoil in the tropical seasonal rainforests within typical peak-cluster depression region of Southwest China. Our results indicated that the SOC content was highest at 400 m asl, which was significantly higher than that at 200 m asl (p < 0.05). Overall, SOC content demonstrated an increasing trend with rising elevation. Additionally, SOC content was significantly higher in the topsoil compared to the subsoil (p < 0.05). The majority of SOC fractions exhibited an increase with elevation but decrease with soil depth. Notably, only water-soluble organic carbon (WSOC) displayed a decrease with elevation. Meanwhile, recalcitrant organic carbon (ROC, 54.27%), particulate organic carbon (POC, 30.19%), and easily oxidizable organic carbon (EOC, 16.95%) were the main SOC fractions. Labile organic carbon (LOC) in the karst forest soil was predominantly composed of EOC and POC. Correlation analysis unveiled significant positive correlations between SOC and certain fractions with elevation, soil total nitrogen, and exchangeable magnesium. Conversely, significant negative correlations were observed with soil bulk density (SBD), soil total phosphorus, and litter phosphorus (Litter P). Redundancy analysis indicated that elevation, SBD, and Litter P were the main environmental variables influencing shifts in SOC and its fractions. Structural equation models showed that SOC was primarily directly impacted by soil properties but indirectly impacted by elevation. ROC was mainly associated with the direct effects of soil properties and litterfall, although elevation exerted a substantial impact through indirect pathways. Moreover, LOC was predominantly influenced by the direct impact of soil properties. Therefore, this study demonstrates that SOC and its fractions are strongly influenced by elevation in karst peak-cluster depression regions and have important implications for forest management and sustainable ecosystem development in these regions.
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- 2024
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14. Instrument Degradation Monitoring with Big Data Analysis of Global Pseudo-Invariant Pixels.
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Junwei Wang, Xiuqing Hu, Ling Wang, Bei Zhang, and Shunxia Miao
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- 2024
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15. Reflecting the Male Gaze: Quantifying Female Objectification in 19th and 20th Century Novels.
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Kexin Luo, Yue Mao, Bei Zhang, and Sophie Hao
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- 2024
16. Identification, Expression Analysis and Cloning of CaSULTR Gene Family in Pepper
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Xiangjie CHENG, Zhiwei GUO, Guangqian REN, Qiang SUN, Hejuan LIU, Junqi ZHEN, Bei ZHANG, Tongtong WANG, Yi LI, and Fusen REN
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pepper ,whole genome analysis ,sulfate transporter ,hormone stress ,expression analysis ,bioinformatics ,Agriculture - Abstract
【Objective】As one of the four major nutrients required by plants, sulfur is a crucial for plant growth and development, playing a pivotal role in enhancing plant resistance against both biotic and abiotic stresses. Sulfate transporters (SULTR) serve as primary regulators in the absorption and transport of sulfate in plants, playing an irreplaceable role in this process. The understanding of the fundamental characteristics and expression patterns of CaSULTR genes could help to provide theoretical support for advancing researches on SULTR in peppers.【Method】Based on the pepper genome, members of the epper CaSULTR gene family were identified at the whole-genome level. A comprehensive analysis of their physicochemical properties, conserved Motifs and gene structures was conducted. By utilizing publicly available transcriptome data and qPCR technology, the expression patterns of CaSULTR genes under various hormone stress conditions were analyzed. Additionally, differentially expressed genes were cloned for further investigation.【Result】A total of 13 CaSULTR genes were identified and designated as CaSULTR1;1~1;3, CaSULTR2;1~2;2, CaSULTR3;1~3;7 and CaSULTR4;1. These genes were distributed on seven chromosomes in pepper, with amino acid lengths ranging from 641 to 947 aa, molecular weights from 69.65 to 104.36 kD, and isoelectric points between 8.42 to 9.62. Characterized by the hydrophobicity, CaSULTR proteins were classified into four subgroups (Ⅰ-Ⅳ), with subgroup Ⅲ containing the highest number of members. Within the same subgroup, members exhibited relatively small differences in physicochemical properties, and Motifs and gene structures were relatively conserved. With the exception of CaSULTR2;1 and CaSULTR2;2, all members possessed Motif 5. Tissue expression analysis indicated that CaSULTR genes may be involved in the development of organs such as leaves, fruits and flowers, with CaSULTR4;1 exhibiting expression in leaves, roots and stems. Expression profile analysis under hormone stress, coupled with qPCR results, revealed significant up-regulation of CaSULTR1;1 and CaSULTR3;4 under hormone induction.【Conclusion】The expression patterns of CaSULTR genes under hormone stress are identified, suggesting that of CaSULTR1;1 and CaSULTR3;4 may be involved in the regulation of sulfur metabolism by exogenous hormones.
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- 2024
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17. Resveratrol activates MAPK/ERK pathway to regulate oestrogen metabolism in type I endometrial cancer
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Qing Wang, Jia-yun Zhou, Li Liu, Ze-yuan Yin, Yan-yu Li, Meng Wang, Jing-bo Zhang, Hui Lu, Xue-yan Zhou, and Bei Zhang
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Resveratrol ,LCMS/MS ,Oestrogen homeostasis ,MAPK pathway ,Network pharmacology ,Endometrial cancer ,Other systems of medicine ,RZ201-999 - Abstract
Abstract Objective Endometrial cancer (EC) is an oestrogen-dependent tumour, the occurrence of which is closely related to an imbalance of oestrogen homeostasis. Our previous studies explored the effects of Resveratrol(Res) on oestrogen metabolism. However, systematic research on the exact mechanism of action of Res is still lacking. Based on network pharmacology, molecular docking and animal experiments, the effects and molecular mechanisms of Res on endometrial cancer were investigated. Methods The target of Res was obtained from the high-throughput experiment and reference-guided database of TCM (HERB) and the Encyclopedia of Traditional Chinese Medicine (ETCM) databases, and the target of endometrial cancer was obtained by using the Genecards database. Venny map was used to obtain the intersection target of Res in the treatment of endometrial cancer, and the protein interaction network of the intersection target was constructed by importing the data into the STRING database. Then, the drug–disease–target interaction network was constructed based on Cytoscape 3.9.1 software. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed for intersection targets using the OmicShare cloud platform. Res and core targets were analysed by molecular docking. EC model mice induced by MNNG were randomly divided into the control group, Res group, MNNG group, MNNG + Res group, and MNNG + Res + MAPK/ERKi group. The protein levels of ERK and p-ERK in the mouse uterus were detected by Western blot. The levels of E1, E2, E3, 16-epiE3, 17-epiE3, 2-MeOE1, 4-MeOE1, 2-MeOE2, 4-MeOE2, 3-MeOE1, 2-OHE1, 4-OHE1, 2-OHE2, 4-OHE2, and 16α-OHE1 in the serum and endometrial tissue of mice were measured by LC‒MS/MS. Results A total of 174 intersection targets of Res anti-endometrial cancer were obtained. The signalling pathways analysed by KEGG enrichment included the AGE-RAGE signalling pathway in diabetic complications, the PI3K-Akt signalling pathway and the MAPK signalling pathway. The top 10 core targets were MAPK3, JUN, TP53, CASP3, TNF, IL1B, AKT1, FOS, VEGFA and INS. Molecular docking showed that in addition to TNF, other targets had good affinity for Res, and the binding activity with MAPK3 was stable. Western blot results showed that Res increased the phosphorylation level of ERK and that MAPK/ERKi decreased ERK activation. In the LC-MS/MS analysis, the levels of 2-MeOE1, 2-MeOE2 and 4-MeOE1 in serum and uterine tissue showed a significantly decreasing trend in the MNNG group, while that of 4-OHE2 was increased (P
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- 2024
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18. Trends in maternal and child health in China and its urban and rural areas from 1991 to 2020: a joinpoint regression model
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Xin‒yue Wang, Bei‒bei Zhang, Yuan‒yi Cao, Qian Xue, Qin Ye, Yuan‒sheng Li, Shu‒yuan Wang, Yuan‒wei Ma, Yan‒que Sun, and Jun‒hui Zhang
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Maternal and child health ,Neonatal mortality rate ,Infant mortality rate ,Under-five mortality rate ,Maternal mortality ratio ,Joinpoint regression model ,Medicine ,Science - Abstract
Abstract The long-term trends in maternal and child health (MCH) in China and the national-level factors that may be associated with these changes have been poorly explored. This study aimed to assess trends in MCH indicators nationally and separately in urban and rural areas and the impact of public policies over a 30‒year period. An ecological study was conducted using data on neonatal mortality rate (NMR), infant mortality rate (IMR), under-five mortality rate (U5MR), and maternal mortality ratio (MMR) nationally and separately in urban and rural areas in China from 1991 to 2020. Joinpoint regression models were used to estimate the annual percentage changes (APC), average annual percentage changes (AAPC) with 95% confidence intervals (CIs), and mortality differences between urban and rural areas. From 1991 to 2020, maternal and child mortalities in China gradually declined (national AAPC [95% CI]: NMRs − 7.7% [− 8.6%, − 6.8%], IMRs − 7.5% [− 8.4%, − 6.6%], U5MRs − 7.5% [− 8.5%, − 6.5%], MMRs − 5.0% [− 5.7%, − 4.4%]). However, the rate of decline nationally in child mortality slowed after 2005, and in maternal mortality after 2013. For all indicators, the decline in mortality was greater in rural areas than in urban areas. The AAPCs in rate differences between rural and urban areas were − 8.5% for NMRs, − 8.6% for IMRs, − 7.7% for U5MRs, and − 9.6% for MMRs. The AAPCs in rate ratios (rural vs. urban) were − 1.2 for NMRs, − 2.1 for IMRs, − 1.7 for U5MRs, and − 1.9 for MMRs. After 2010, urban‒rural disparity in MMR did not diminish and in NMR, IMR, and U5MR, it gradually narrowed but persisted. MCH indicators have declined at the national level as well as separately in urban and rural areas but may have reached a plateau. Urban‒rural disparities in MCH indicators have narrowed but still exist. Regular analyses of temporal trends in MCH are necessary to assess the effectiveness of measures for timely adjustments.
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- 2024
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19. Efficiency of copy number variation sequencing combined with karyotyping in fetuses with congenital heart disease and the following outcomes
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Xuezhen Wang, Jing Sha, Yu Han, Min Pang, Min Liu, Mengna Liu, Bei Zhang, and Jingfang Zhai
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Congenital heart diseases ,Genetic etiology ,Karyotype analysis ,Copy number variation sequencing ,Genetics ,QH426-470 - Abstract
Abstract Background Both copy number variant-sequencing (CNV-seq) and karyotype analysis have been used as powerful tools in the genetic aetiology of fetuses with congenital heart diseases (CHD). However, CNV-seq brings clinicians more confusions to interpret the detection results related to CHD with or without extracardiac abnormalities. Hence, we conducted this study to investigate the clinical value of CNV-seq in fetuses with CHD. Results A total of 167 patients with fetal CHD including 36 single CHD (sCHD), 41 compound CHD (cCHD) and 90 non-isolated CHD (niCHD) were recruited into the study. 28 cases (16.77%, 28/167) were revealed with chromosomal abnormalities at the level of karyotype. The pathogenic detection rate (DR) of CNV-seq (23.17%, 19/82) was higher than that of karyotyping (15.85%, 13/82) in 82 cases by CNV-seq and karyotyping simultaneously. The DR of pathogenic copy number variations (PCNVs) (31.43%) was higher in niCHD subgroup than that in sCHD and cCHD (9.52% and 23.08%). Conotruncal defect (CTD) was one of the most common heart malformations with the highest DR of PCNVs (50%) in 7 categories of CHD. In terms of all the pregnancy outcomes, 67 (40.12%) cases were terminated and 100 (59.88%) cases were live neonates. Only two among 34 cases with a pathogenic genetic result chose to continue the pregnancy. Conclusions CNV-seq combined with karyotyping is a reliable and accurate prenatal technique for identifying pathogenic chromosomal abnormalities associated with fetal CHD with or without extracardiac abnormalities, which can assist clinicians to perform detailed genetic counselling with regard to the etiology and related outcomes of CHD.
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- 2024
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20. Existence of solutions of coupled φ-Hilfer fractional differential systems with integral boundary conditions
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Bei ZHANG, Huanmin SI, Weihua JIANG, Chunjing GUO, and Kun CHEN
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analytic theory ,φ-hilfer fractional order derivative ,coupling system ,the principle of compressed images ,kransnoselskii's fixed point theorem ,existence of solutions ,Technology - Abstract
In order to expand the relevant theory of fractional differential equation systems, a class of coupled φ-Hilfer fractional differential systems with integral boundary conditions was studied. Firstly, the coupled φ-Hilfer fractional differential system with integral boundary conditions was transformed into an integral system. Secondly, the appropriate Banach product space and norm were defined, the appropriate integral operator was constructed, and the existence result of the solution of the coupled φ-Hilfer fractional differential system under the integral boundary condition was given by using the compressed image principle and Kransnoselskii's fixed point theorem, respectively. Finally, examples were given to illustrate the correctness of the conclusions obtained. The results show that the solutions of the coupled φ-Hilfer fractional differential system under the integral boundary condition exist. The existence of solutions of coupled φ-Hilfer fractional differential systems is studied for the first time by using the compressed image principle and Kransnoselskii's fixed point theorem, respectively, and some innovative new results are obtained. In addition, the research conclusion enriches the relevant theories of the theoretical solvability of coupled fractional differential systems, and provides certain theoretical reference value for the further study of fractional order differential equations.
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- 2024
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21. Risk factors investigation for different outcomes between unilateral and bilateral chronic rhinosinusitis with nasal polyps patients
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Jianwei Wang, Yu Zhang, Ying Chen, Xinjun Xu, Yujuan Yang, Jiali Yin, Jing Guo, Pengyi Yu, Zhen Liu, Huifang Liu, Ting Zuo, Hongfei Zhao, Yan Hao, Bei Zhang, and Xicheng Song
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bilateral ,chronic rhinosinusitis with nasal polyps ,disease control ,recurrence ,unilateral ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Abstract Background Studies involving chronic rhinosinusitis with nasal polyps (CRSwNP) have mostly focused on bilateral cases, making unilateral CRSwNP inadequately recognized. This study examined the differences in clinical characteristics, outcomes, and risk factors for poor outcomes between unilateral and bilateral CRSwNP to facilitate a better assessment in the two groups. Methods Demographic information, tissue and blood cells, endoscopic scores, Lund‐Mackay scores, recurrence rates, and disease control conditions were compared between 310 unilateral and 596 bilateral CRSwNP patients. Furthermore, the stepwise regression multivariate Cox proportional hazard models were performed to generate risk factors for poor outcomes in the two groups. Results Bilateral cases exhibited higher rates of smoking, AR, and asthma comorbidities, along with higher numbers of tissue eosinophils and blood inflammatory cells when compared to unilateral patients. Endoscopic nasal polyp score, total computed tomography (CT) score (with scores for each sinus cavity), and adjusted CT scores were significantly higher in the bilateral group, except for a markedly higher adjusted maxillary score in the unilateral group. Furthermore, significantly higher proportions of bilateral patients experienced nasal polyp recurrence, uncontrolled status, and most disease control‐related symptoms at follow‐up. The primary risk factors for poor outcomes were asthma, tissue eosinophils, and total CT score in the bilateral group and blood basophils in the unilateral group. Conclusions Bilateral CRSwNP patients experience worse disease severity and outcomes than their unilateral counterparts. Primarily, asthma, tissue eosinophils, and total CT score were risk factors for poor outcomes in bilateral CRSwNP patients, with blood basophils in unilateral cases.
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- 2024
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22. Integrative metagenomic and lipidomic analyses reveal alterations in children with obesity and after lifestyle intervention
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Chunyan Yin, Lujie Liu, Dong Xu, Meng Li, Min Li, Yujie Qin, Bei Zhang, Yongfa Sun, Yuesheng Liu, and Yanfeng Xiao
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childhood ,obesity ,metagenomics ,lipidomic ,weight loss ,Nutrition. Foods and food supply ,TX341-641 - Abstract
BackgroundDespite emerging evidence linking alterations in gut microbiota to childhood obesity, the metabolic mechanisms linking gut microbiota to the lipid profile during childhood obesity and weight loss remain poorly understood.MethodologyIn this study, children with obesity were treated with lifestyle weight loss therapy. Metagenomics association studies and serum untargeted lipidomics analyses were performed in children with obesity and healthy controls before and after weight loss.Main findingsWe identified alterations in gut microbiota associated with childhood obesity, as well as variations in circulating metabolite concentrations. Children with obesity showed significant decreases in the levels of s-Rothia_kristinae and s-Enterobacter_roggenkampii, alongsige elevated levels of s-Clostridiales_bacterium_Marseille-P5551. Following weight loss, the levels of s-Streptococcus_infantarius and s-Leuconostoc_citreum increased by factors of 3.354 and 1.505, respectively, in comparison to their pre-weight loss levels. Correlation analyses indicated a significant positive relationship between ChE(2:0) levels and both with s-Lachnospiraceae_bacterium_TF09-5 and fasting glucose levels. CoQ8 levels were significantly negatively correlated with s-Rothia_kristinae and HOMA-IR.ConclusionWe linked altered gut microbiota and serum lipid levels in children with obesity to clinical indicators, indicating a potential impact on glucose metabolism via lipids. This study contributes to understanding the mechanistic relationship between altered gut microbiota and childhood obesity and weight loss, suggesting gut microbiome as a promising target for intervention.Clinical trial registrationhttps://www.chictr.org.cn/showproj.html?proj=178971, ChiCTR2300072179.
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- 2024
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23. Spatiotemporal alterations in the brain oscillations of Arctic explorers
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Yong-Bo Hu, Jing Lu, Hong-Xia Li, Craig S. Anderson, Zhong-Min Liu, Bei Zhang, and Jun-Jie Hao
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Arctic explorers ,Electroencephalography ,Brain oscillations ,Theta oscillations ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
Background: The limited understanding of the physiology and psychology of polar expedition explorers has prompted concern over the potential cognitive impairments caused by exposure to extreme environmental conditions. Prior research has demonstrated that such stressors can negatively impact cognitive function, sleep quality, and behavioral outcomes. Nevertheless, the impact of the polar environment on neuronal activity remains largely unknown. Methods: In this study, we aimed to investigate spatiotemporal alterations in brain oscillations of 13 individuals (age range: 22–48 years) who participated in an Arctic expedition. We utilized electroencephalography (EEG) to record cortical activity before and during the Arctic journey, and employed standardized low resolution brain electromagnetic tomography to localize changes in alpha, beta, theta, and gamma activity. Results: Our results reveal a significant increase in the power of theta oscillations in specific regions of the Arctic, which differed significantly from pre-expedition measurements. Furthermore, microstate analysis demonstrated a significant reduction in the duration of microstates (MS) D and alterations in the local synchrony of the frontoparietal network. Conclusion: Overall, these findings provide novel insights into the neural mechanisms underlying adaptation to extreme environments. These findings have implications for understanding the cognitive consequences of polar exploration and may inform strategies to mitigate potential neurological risks associated with such endeavors. Further research is warranted to elucidate the long-term effects of Arctic exposure on brain function.
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- 2024
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24. Frailty and its Associated Factors Among Rural Community-Dwelling Older Adults: A Cross-Sectional Study
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Shaobo Guo MSN(c), RN, Hongxia Liu PhD, RN, Bei Zhang MD, Xiangru Li MSN(c), RN, and Keke Lin PhD, RN
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Public aspects of medicine ,RA1-1270 - Abstract
To investigate the status quo and influencing factors of overall frailty and its 3 domains among rural community-dwelling older adults. This is a cross-sectional study. A convenience sample of 195 older adults from 6 villages in Bashang Area of Zhangjiakou City, Hebei Province, China, were recruited from August to September, 2022. The demographic characteristics, the Chinese version of Tilburg Frailty Indicator, Charlson Comorbidity Scale and Hospital Anxiety and Depression Scale were used to investigate frailty and its influencing factors. Univariate analysis and multiple linear regression analysis were employed. The prevalence of overall frailty among the older adults in Bashang Area was 85.13%. Multiple linear regression analysis showed that age, gender, marital status, regular exercise, comorbidity, and anxiety were the influencing factors of overall frailty. While anxiety was the only shared influencing factor for physical frailty, psychological frailty, and social frailty, age, gender, marital status, financial burden, the comorbidity, and regular exercise were factors which influenced 1 or 2 domains of frailty. The prevalence of overall frailty among the older adults in rural areas, Zhangjiakou City is high. It is influenced by many factors. Medical staff and policy makers should work hand in hand to improve frailty among rural community-dwelling older adults in China.
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- 2024
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25. Effects of melatonin on inhibiting quality deterioration of postharvest water bamboo shoots
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Chunlu Qian, Yan Sun, Bei Zhang, Yuyang Shao, Jun Liu, Juan Kan, Man Zhang, Lixia Xiao, Changhai Jin, and Xiaohua Qi
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Water bamboo shoots ,Cold storage ,Melatonin ,Chlorophyll ,Lignin ,Nutrition. Foods and food supply ,TX341-641 - Abstract
Water bamboo shoots (Zizania latifolia) is prone to quality deterioration during cold storage after harvest, which causes the decline of commodity value. Chlorophyll synthesis and lignin deposition are the major reasons for quality degradation. This paper studied the influence of exogenous melatonin (MT) on the cold storage quality of water bamboo shoots. MT treatment could delay the increase in skin browning, hardness and weight loss rate, inhibit chlorophyll synthesis and color change of water bamboo shoots, while maintain the content of total phenols and flavonoids, and inhibit lignin deposition by inhibiting the activity and gene expression of phenylpropanoid metabolism related enzymes as PAL, C4H, 4CL, CAD, and POD. The results indicate that exogenous MT treatment can effectively inhibit the quality degradation of cold stored water bamboo shoots.
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- 2024
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26. Adhesion between EVs and tumor cells facilitated EV-encapsulated doxorubicin delivery via ICAM1
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Shibo Wang, Chenxiao Qiao, Xianghui Kong, Jie Yang, Fei Guo, Jiming Chen, Wenhui Wang, Bei Zhang, Huiqing Xiu, Yuzhou He, Jianli Wang, Huajun Feng, and Zhijian Cai
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Extracellular vesicles ,Doxorubicin ,ICAM1 ,IFN-γ ,TNF-α ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Doxorubicin (Dox) is an anti-tumor drug with a broad spectrum, whereas the cardiotoxicity limits its further application. In clinical settings, liposome delivery vehicles are used to reduce Dox cardiotoxicity. Here, we substitute extracellular vesicles (EVs) for liposomes and deeply investigate the mechanism for EV-encapsulated Dox delivery. The results demonstrate that EVs dramatically increase import efficiency and anti-tumor effects of Dox in vitro and in vivo, and the efficiency increase benefits from its unique entry pattern. Dox-loading EVs repeat a “kiss-and-run” motion before EVs internalization. Once EVs touch the cell membrane, Dox disassociates from EVs and directly enters the cytoplasm, leading to higher and faster Dox import than single Dox. This unique entry pattern makes the adhesion between EVs and cell membrane rather than the total amount of EV internalization the key factor for regulating the Dox import. Furthermore, we recognize ICAM1 as the molecule mediating the adhesion between EVs and cell membranes. Interestingly, EV-encapsulated Dox can induce ICAM1 expression by irritating IFN-γ and TNF-α secretion in TME, thereby increasing tumor targeting of Dox-loading EVs. Altogether, EVs and EV-encapsulated Dox synergize via ICAM1, which collectively enhances the curative effects for tumor treatment.
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- 2024
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27. Quantitative Pixel-Level Segmentation and 3D Reconstruction of Concealed Cracks in Asphalt Pavements.
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Haoyuan Cheng, Bei Zhang, Yanhui Zhong, and Shengjie Xu
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- 2024
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28. Vortex Retarder Allows Broadband Polarization Direction Indicator.
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Bei Zhang, Xinyun Zhu, and Yiwei Dong
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- 2024
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29. Sustainable Scientific and Technological Talents Recommendation Method Based on Recommendation Algorithm.
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Bei Zhang
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- 2024
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30. The Hermite-type virtual element method for second order problem.
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Jikun Zhao, Fengchen Zhou, Bei Zhang, and Xiaojing Dong
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- 2024
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31. Real-Time Detection of Voids in Asphalt Pavement Based on Swin-Transformer-Improved YOLOv5.
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Bei Zhang, Haoyuan Cheng, Yanhui Zhong, Jing Chi, Guoyin Shen, Zhaoxu Yang, Xiaolong Li, and Shengjie Xu
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- 2024
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32. Robust Vascular Segmentation for Raw Complex Images of Laser Speckle Contrast Based on Weakly Supervised Learning.
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Suzhong Fu, Jing Xu, Shilong Chang, Luyao Yang, Shuting Ling, Jinghan Cai, Jiayin Chen, Jiacheng Yuan, Ying Cai, Bei Zhang, Zicheng Huang, Kun Yang, Wenhai Sui, Linyan Xue, and Qingliang Zhao
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- 2024
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33. A Novel Dual-LC Resonant DC Circuit Breaker Employing Soft-Switching Technique for Enhanced Performance.
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Bei Zhang, Xiangyu Zhang 0004, Sihang Wu, Liangtao Zhan, and Lei Qi 0005
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- 2024
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34. A Novel Low-Cost DC Chopper for Offshore Wind VSC-HVDC System by Using Thyristors Instead of Full-Control Devices.
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Sihang Wu, Lei Qi 0005, Xiangyu Zhang 0004, Bei Zhang, Zhiguo He, and Meichen Jin
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- 2024
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35. Research on improved small scale face detection algorithm based on Yolov5.
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Bei Zhang, Yuan Cao, Changqing Wang, and Meng Wang
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- 2024
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36. Determination of ammonia nitrogen by surface-enhanced Raman spectroscopy and DFT studies
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Xiaofeng, Zhang, Yibin, Liu, Yilong, Zhong, and Bei, Zhang
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- 2024
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37. First-line camrelizumab (a PD-1 inhibitor) plus apatinib (an VEGFR-2 inhibitor) and chemotherapy for advanced gastric cancer (SPACE): a phase 1 study
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Xiaofeng Chen, Hao Xu, Xiaobing Chen, Tongpeng Xu, Yitong Tian, Deqiang Wang, Fen Guo, Kangxin Wang, Guangfu Jin, Xiao Li, Rong Wang, Fengyuan Li, Yongbin Ding, Jie Tang, Yueyu Fang, Jing Zhao, Liang Liu, Ling Ma, Lijuan Meng, Zhiguo Hou, Rongrong Zheng, Yang Liu, Ni Guan, Bei Zhang, Shuang Tong, Shiqing Chen, Xing Li, and Yongqian Shu
- Subjects
Medicine ,Biology (General) ,QH301-705.5 - Abstract
Abstract Patients with advanced gastric cancer typically face a grim prognosis. This phase 1a (dose escalation) and phase 1b (dose expansion) study investigated safety and efficacy of first-line camrelizumab plus apatinib and chemotherapy for advanced gastric or gastroesophageal junction adenocarcinoma. The primary endpoints included maximum tolerated dose (MTD) in phase 1a and objective response rate (ORR) across phase 1a and 1b. Phase 1a tested three dose regimens of camrelizumab, apatinib, oxaliplatin, and S-1. Dose regimen 1: camrelizumab 200 mg on day 1, apatinib 250 mg every other day, oxaliplatin 100 mg/m² on day 1, and S-1 40 mg twice a day on days 1–14. Dose regimen 2: same as dose regimen 1, but oxaliplatin 130 mg/m². Dose regimen 3: same as dose regimen 2, but apatinib 250 mg daily. Thirty-four patients were included (9 in phase 1a, 25 in phase 1b). No dose-limiting toxicities occurred so no MTD was identified. Dose 3 was set for the recommended phase 2 doses and administered in phase 1b. The confirmed ORR was 76.5% (95% CI 58.8–89.3). The median progression-free survival was 8.4 months (95% CI 5.9-not evaluable [NE]), and the median overall survival (OS) was not mature (11.6-NE). Ten patients underwent surgery after treatment and the multidisciplinary team evaluation. Among 24 patients without surgery, the median OS was 19.6 months (7.8-NE). Eighteen patients (52.9%) developed grade ≥ 3 treatment-emergent adverse events. Camrelizumab plus apatinib and chemotherapy showed favorable clinical outcomes and manageable safety for untreated advanced gastric cancer (ChiCTR2000034109).
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- 2024
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38. Association of tet methylcytosine dioxygenase 2 and 5-hydroxymethylcytosine in endometrioid adenocarcinoma and its clinical significance
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Lei Kuang, Jingbo Zhang, Yanyu Li, Qing Wang, Jianwei Liu, and Bei Zhang
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Endometrial cancer ,Tet methylcytosine dioxygenase 2 ,5-hydroxymethylcytosine ,Association ,Prognosis ,Gynecology and obstetrics ,RG1-991 ,Public aspects of medicine ,RA1-1270 - Abstract
Abstract Background Aberrant DNA methylation is a vital molecular alteration commonly detected in type I endometrial cancers (EC), and tet methylcytosine dioxygenase 2 (TET2) and 5-hydroxymethylcytosine (5hmC) play significant roles in DNA demethylation. However, little is known about the function and correlation of TET2 and 5hmC co-expressed in EC. This study intended to investigate the clinical significance of TET2 and 5hmC in EC. Methods The levels of TET2 and 5hmC were detected in 326 endometrial tissues by immumohistochemistry, and the correlation of their level was detected by Pearson analysis. The association between the levels of TET2 and 5hmC and clinicopathologic characteristics was analyzed. Prognostic value of TET2 and 5hmC was explored by Kaplan–Meier analysis. The Cox proportional hazard regression model was used for univariate and multivariate analyses. Results Based on the analysis results, TET2 protein level was positively correlated with 5hmC level in EC tissues (r = 0.801, P
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- 2024
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39. A MRI radiomics-based model for prediction of pelvic lymph node metastasis in cervical cancer
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Tao Wang, Yan-Yu Li, Nan-Nan Ma, Pei-An Wang, and Bei Zhang
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MRI ,Radiomics ,Lymph node ,Cervical cancer ,Surgery ,RD1-811 ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Background Cervical cancer (CC) is a common malignancy of the female reproductive tract, and preoperative prediction of lymph node metastasis (LNM) is essential. This study aims to design and validate a magnetic resonance imaging (MRI) radiomics-based predictive model capable of detecting LNM in patients diagnosed with CC. Methods This retrospective analysis incorporated 86 and 38 CC patients into the training and testing groups, respectively. Radiomics features were extracted from MRI T2WI, T2WI-SPAIR, and axial apparent diffusion coefficient (ADC) sequences. Selected features identified in the training group were then used to construct a radiomics scoring model, with relevant LNM-related risk factors having been identified through univariate and multivariate logistic regression analyses. The resultant predictive model was then validated in the testing cohort. Results In total, 16 features were selected for the construction of a radiomics scoring model. LNM-related risk factors included worse differentiation (P < 0.001), more advanced International Federation of Gynecology and Obstetrics (FIGO) stages (P = 0.03), and a higher radiomics score from the combined MRI sequences (P = 0.01). The equation for the predictive model was as follows: −0.0493–2.1410 × differentiation level + 7.7203 × radiomics score of combined sequences + 1.6752 × FIGO stage. The respective area under the curve (AUC) values for the T2WI radiomics score, T2WI-SPAIR radiomics score, ADC radiomics score, combined sequence radiomics score, and predictive model were 0.656, 0.664, 0.658, 0.835, and 0.923 in the training cohort, while these corresponding AUC values were 0.643, 0.525, 0.513, 0.826, and 0.82 in the testing cohort. Conclusions This MRI radiomics-based model exhibited favorable accuracy when used to predict LNM in patients with CC. Relative to the use of any individual MRI sequence-based radiomics score, this predictive model yielded superior diagnostic accuracy.
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- 2024
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40. Gut microbiota contributes to bisphenol A-induced maternal intestinal and placental apoptosis, oxidative stress, and fetal growth restriction in pregnant ewe model by regulating gut-placental axis
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Hao Zhang, Xia Zha, Bei Zhang, Yi Zheng, Mabrouk Elsabagh, Hongrong Wang, and Mengzhi Wang
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Bisphenol A ,Fetal growth restriction ,Gut microbiota ,Gut-placental axis ,Pregnant ewe ,Microbial ecology ,QR100-130 - Abstract
Abstract Background Bisphenol A (BPA) is an environmental contaminant with endocrine-disrupting properties that induce fetal growth restriction (FGR). Previous studies on pregnant ewes revealed that BPA exposure causes placental apoptosis and oxidative stress (OS) and decreases placental efficiency, consequently leading to FGR. Nonetheless, the response of gut microbiota to BPA exposure and its role in aggravating BPA-mediated apoptosis, autophagy, mitochondrial dysfunction, endoplasmic reticulum stress (ERS), and OS of the maternal placenta and intestine are unclear in an ovine model of gestation. Results Two pregnant ewe groups (n = 8/group) were given either a subcutaneous (sc) injection of corn oil (CON group) or BPA (5 mg/kg/day) dissolved in corn oil (BPA group) once daily, from day 40 to day 110 of gestation. The maternal colonic digesta and the ileum and placental tissue samples were collected to measure the biomarkers of autophagy, apoptosis, mitochondrial dysfunction, ERS, and OS. To investigate the link between gut microbiota and the BPA-induced FGR in pregnant ewes, gut microbiota transplantation (GMT) was conducted in two pregnant mice groups (n = 10/group) from day 0 to day 18 of gestation after removing their intestinal microbiota by antibiotics. The results indicated that BPA aggravates apoptosis, ERS and autophagy, mitochondrial function injury of the placenta and ileum, and gut microbiota dysbiosis in pregnant ewes. GMT indicated that BPA-induced ERS, autophagy, and apoptosis in the ileum and placenta are attributed to gut microbiota dysbiosis resulting from BPA exposure. Conclusions Our findings indicate the underlying role of gut microbiota dysbiosis and gut-placental axis behind the BPA-mediated maternal intestinal and placental apoptosis, OS, and FGR. The findings further provide novel insights into modulating the balance of gut microbiota through medication or probiotics, functioning via the gut-placental axis, to alleviate gut-derived placental impairment or FGR. Video Abstract
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- 2024
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41. Genome-wide CRISPR screen identifies ESPL1 limits the response of gastric cancer cells to apatinib
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Bei Zhang, Yan Chen, Xinqi Chen, Zhiyao Ren, Hong Xiang, Lipeng Mao, and Guodong Zhu
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Apatinib resistance ,ESPL1 ,CRISPR screening ,MDM2 ,Gastric cancer ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 ,Cytology ,QH573-671 - Abstract
Abstract Apatinib was the first anti-angiogenic agent approved for treatment of metastatic gastric cancer (GC). However, the emergence of resistance was inevitable. Thus investigating new and valuable off-target effect of apatinib directly against cancer cells is of great significance. Here, we identified extra spindle pole bodies-like 1 (ESPL1) was responsible for apatinib resistance in GC cells through CRISPR genome-wide gain-of-function screening. Loss of function studies further showed that ESPL1 inhibition suppressed cell proliferation, migration and promoted apoptosis in vitro, and accordingly ESPL1 knockdown sensitized GC cells to apatinib. In addition, we found ESPL1 interacted with mouse double minute 2 (MDM2), a E3 ubiquitin protein ligase, and the combination of MDM2 siRNA with apatinib synergistically ameliorated the resistance induced by ESPL1 overexpression. In summary, our study indicated that ESPL1 played a critical role in apatinib resistance in GC cells. Inhibition of MDM2 could rescue the sensitivity of GC cells to apatinib and reverse ESPL1-mediated resistance.
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- 2024
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42. Comparative Evaluation of Structural Characteristics of Starch from 10 Varieties of Lotus Root
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Fei Wang, Minghui Xu, Yaying Jiang, Bei Zhang, Yuyang Shao, Jun Liu, Juan Kan, Man Zhang, Lixia Xiao, Xiaohua Qi, Liangjun Li, Shuping Zhao, and Chunlu Qian
- Subjects
lotus root ,starch ,physicochemical property ,structural characteristics ,Plant culture ,SB1-1110 - Abstract
Starch extracted from ten lotus root varieties (Yeyong, MRHZ, Zhonghua, Suiningbaiou, Zaohua, Meirenhong, E9:11, Huchengyeou, Peixianyeou, Xin No. 5) from Jiangsu Province, China, were studied. The structural characteristics of lotus root starch were analyzed based on its physical and chemical properties, particle structure, crystal structure, layered structure, thermal properties and gel properties. The lotus root starches were generally milky white, with a starch content ranging from 81.72 to 99.54%, amylose content ranging from 15.44 to 23.54%, light transmittance between 15.30 and 26.90%, and swelling power and solubility at 22.15–29.72% and 2.00–6.00%, respectively. Among the varieties, “Suiningbaiou” had the whitest starch, while “Meirenhong” and “Suiningbaiou” had higher starch contents. “Huchengyeou” had the lowest amylose content, and “Zhonghua” showed the highest light transmittance. “Yeyong” and “Zaohua” had higher solubility, whereas “Zhonghua” had the highest swelling power. An optical, polarizing and scanning electron microscope revealed that most of the lotus starch was oval. However, starch from “Zhonghua”, “E9:11”, “Peixianyeou” and “Zaohua” was longer, whereas starch from “Meirenhong”, “MRHZ”, “Huchengyeou”, “Yeyong”, “Suiningbaiou” and “Xin No.5” was shorter. The X-ray powder diffraction patterns and 13C NMR spectroscopy indicated that “Zaohua” starch exhibited type A crystallization, while starch from the other varieties displayed type CA crystallization, with a relative crystallinity between 18.27 and 22.10%; “Huchengyeou” and “Yeyong” had higher crystallinity. Infrared spectroscopy and small-angle X diffraction analysis showed similar results for short-range order and lamella spacing. The thermal and gel properties of starch from the ten lotus root varieties also varied. The enthalpy change values of “Xin No.5” and “Yeyong” were higher, and starch from “Yeyong” and “Huchengyeou” showed a greater gel strength. Finally, factor score analysis ranked the starch quality of the top four lotus root varieties in Jiangsu as “Suiningbaiou”, “Yeyong”, “Huchengyeou”, and “Zaohua”.
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- 2024
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43. Association between machine learning-assisted heavy metal exposures and diabetic kidney disease: a cross-sectional survey and Mendelian randomization analysis
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Ruiqi Zhao, Sen Lin, Mengyao Han, Zhimei Lin, Mengjiao Yu, Bei Zhang, Lanyue Ma, Danfei Li, and Lisheng Peng
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environmental epidemiology ,heavy metal exposures ,diabetic kidney disease ,NHANES ,machine learning ,Mendelian randomization ,Public aspects of medicine ,RA1-1270 - Abstract
Background and objectiveHeavy metals, ubiquitous in the environment, pose a global public health concern. The correlation between these and diabetic kidney disease (DKD) remains unclear. Our objective was to explore the correlation between heavy metal exposures and the incidence of DKD.MethodsWe analyzed data from the NHANES (2005–2020), using machine learning, and cross-sectional survey. Our study also involved a bidirectional two-sample Mendelian randomization (MR) analysis.ResultsMachine learning reveals correlation coefficients of −0.5059 and − 0.6510 for urinary Ba and urinary Tl with DKD, respectively. Multifactorial logistic regression implicates urinary Ba, urinary Pb, blood Cd, and blood Pb as potential associates of DKD. When adjusted for all covariates, the odds ratios and 95% confidence intervals are 0.87 (0.78, 0.98) (p = 0.023), 0.70 (0.53, 0.92) (p = 0.012), 0.53 (0.34, 0.82) (p = 0.005), and 0.76 (0.64, 0.90) (p = 0.002) in order. Furthermore, multiplicative interactions between urinary Ba and urinary Sb, urinary Cd and urinary Co, urinary Cd and urinary Pb, and blood Cd and blood Hg might be present. Among the diabetic population, the OR of urinary Tl with DKD is a mere 0.10, with a 95%CI of (0.01, 0.74), urinary Co 0.73 (0.54, 0.98) in Model 3, and urinary Pb 0.72 (0.55, 0.95) in Model 2. Restricted Cubic Splines (RCS) indicate a linear linkage between blood Cd in the general population and urinary Co, urinary Pb, and urinary Tl with DKD among diabetics. An observable trend effect is present between urinary Pb and urinary Tl with DKD. MR analysis reveals odds ratios and 95% confidence intervals of 1.16 (1.03, 1.32) (p = 0.018) and 1.17 (1.00, 1.36) (p = 0.044) for blood Cd and blood Mn, respectively.ConclusionIn the general population, urinary Ba demonstrates a nonlinear inverse association with DKD, whereas in the diabetic population, urinary Tl displays a linear inverse relationship with DKD.
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- 2024
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44. Changes in physio-biochemical metabolism, phenolics and antioxidant capacity during germination of different wheat varieties
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Wenxin Li, Xiaoyong Liu, Yan Ma, Xianqing Huang, Dan Hai, Yongxia Cheng, Ge Bai, Yinping Wang, Bei Zhang, Mingwu Qiao, Lianjun Song, and Ning Li
- Subjects
Germination ,Wheat sprouts ,Physio-biochemical metabolism ,Phenolic acids ,Flavonoids ,Antioxidant capacity ,Nutrition. Foods and food supply ,TX341-641 ,Food processing and manufacture ,TP368-456 - Abstract
Changes in physio-biochemical metabolism, phenolics and antioxidant capacity during germination were studied in eight different wheat varieties. Results showed that germination enhanced sprout growth, and caused oxidative damage, but enhanced phenolics accumulation. Ferulic acid and p-coumaric acid were the main phenolic acids in wheat sprouts, and dihydroquercetin, quercetin and vitexin were the main flavonoids. The phenolic acid content of Jimai 44 was the highest on the 2th and 4th day of germination, and that of Bainong 307 was the highest on the 6th day. The flavonoid content of Hei jingang was the highest during whole germination. The enzymes activities of phenylalanine ammonia lyase (PAL), cinnamic acid 4-hydroxylase (C4H) and 4-coumarate coenzyme A ligase (4CL) were up-regulated. The activities of catalase, polyphenol oxidase and peroxidase were also activated. Antioxidant capacity of wheat sprouts was enhanced. The results provided new ideas for the production of naturally sourced phenolic rich foods.
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- 2024
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45. In Situ Formation of Fibronectin‐Enriched Protein Corona on Epigenetic Nanocarrier for Enhanced Synthetic Lethal Therapy
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Zhangyi Luo, Zhuoya Wan, Pengfei Ren, Bei Zhang, Yixian Huang, Raymond E. West III, Haozhe Huang, Yuang Chen, Thomas D. Nolin, Wen Xie, Junmei Wang, Song Li, and Jingjing Sun
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immunotherapy ,nanocarrier ,protein corona ,synthetic lethal therapy ,ultra‐Small ,Science - Abstract
Abstract PARP inhibitors (PARPi)‐based synthetic lethal therapy demonstrates limited efficacy for most cancer types that are homologous recombination (HR) proficient. To potentiate the PARPi application, a nanocarrier based on 5‐azacytidine (AZA)‐conjugated polymer (PAZA) for the codelivery of AZA and a PARP inhibitor, BMN673 (BMN) is developed. AZA conjugation significantly decreased the nanoparticle (NP) size and increased BMN loading. Molecular dynamics simulation and experimental validations shed mechanistic insights into the self‐assembly of effective NPs. The small PAZA NPs demonstrated higher efficiency of tumor targeting and penetration than larger NPs, which is mediated by a new mechanism of active targeting that involves the recruitment of fibronectin from serum proteins following systemic administration of PAZA NPs. Furthermore, it is found that PAZA carrier sensitize the HR‐proficient nonsmall cell lung cancer (NSCLC) to BMN, a combination therapy that is more effective at a lower AZA/BMN dosage. To investigate the underlying mechanism, the tumor immune microenvironment and various gene expressions by RNAseq are explored. Moreover, the BMN/PAZA combination increased the immunogenicity and synergized with PD‐1 antibody in improving the overall therapeutic effect in an orthotopic model of lung cancer (LLC).
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- 2024
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46. Exploring opportunities to strengthen rural tuberculosis health service delivery: a qualitative study with health workers in Tibet autonomous region, China
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Xiaolin Wei, Bei Zhang, Jun Hu, Victoria Haldane, Zhitong Zhang, Katie N Dainty, Elizabeth Rea, Pande Pasang, Tingting Yin, Yinlong Li, and Qiuyu Pan
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Medicine - Abstract
Objectives This qualitative study aimed to explore opportunities to strengthen tuberculosis (TB) health service delivery from the perspectives of health workers providing TB care in Shigatse prefecture of Tibet Autonomous Region, China.Design Qualitative research, semi-structured in-depth interviews.Setting The TB care ecosystem in Shigatse, including primary and community care.Participants Participants: 37 semi-structured interviews were conducted with village doctors (14), township doctors and nurses (14), county hospital doctors (7) and Shigatse Centre for Disease Control staff (2).Results The three main themes reported include (1) the importance of training primary and community health workers to identify people with symptoms of TB, ensure TB is diagnosed and link people with TB to further care; (2) the need to engage community health workers to ensure retention in care and adherence to TB medications; and (3) the opportunity for innovative technologies to support coordinated care, retention in care and adherence to medication in Shigatse.Conclusions The quality of TB care could be improved across the care cascade in Tibet and other high-burden, remote settings by strengthening primary care through ongoing training, greater support and inclusion of community health workers and by leveraging technology to create a circle of care. Future formative and implementation research should include the perspectives of health workers at all levels to improve care organisation and delivery.
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- 2024
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47. Inhibition of inosine metabolism of the gut microbiota decreases testosterone secretion in the testis
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Lei Tang, Xizhong Yang, Mengting Zhou, Lingxin Feng, Cuijie Ji, Jie Liang, Bei Zhang, Ruowu Shen, and Luoyang Wang
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antibiotic ,gut microbiota ,testosterone ,colistin ,inosine ,Akkermansia ,Microbiology ,QR1-502 - Abstract
ABSTRACTGrowing evidence indicates that gut microbiota is involved in the regulation of the host’s sex hormone levels, such as through interfering with the sex hormone metabolism in the intestine. However, if gut microbiota or its metabolites directly influence the sex hormone biosynthesis in the gonad remains largely unknown. Our previous study showed that colistin, as a narrow-spectrum antibiotic, can significantly downregulate the serum testosterone levels and thus enhance the antitumor efficiency of anti-PD-L1 in male mice; however, the underlying mechanism for the regulation of the host’s testosterone levels remains uninvestigated. In the present study, we analyzed the impact of colistin on the immune microenvironment of the testis as well as the composition and metabolism of gut microbiota in male mice. Our results showed that colistin has an impact on the immune microenvironment of the testis and can downregulate serum testosterone levels in male mice through inhibition of Akkermansia, leading to destroyed inosine metabolism. Supplement with inosine can restore testosterone secretion probably by prompting the recovery of the intestinal mucus barrier and the serum lipopolysaccharides levels. All these findings reveal a new pathway for the regulation of the host’s sex hormone levels by gut microbiota.IMPORTANCEThis study demonstrates that exposure to even narrow-spectrum antibiotics may affect the host’s testosterone levels by altering the gut microbiota and its metabolites. Our findings provide evidence that some specific gut bacteria have an impact on the sex hormone biosynthesis in the testis.
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- 2024
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48. Design and synthesis of chitosan/calcium lignosulfonate/Au NPs: Its performance for reduction of nitro compounds and in the treatment of cancer
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Ruyi Xie, Jianming Zhou, Ting Wang, Yuan Xu, Bei Zhang, Sally Negm, and Attalla F. Elkott
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Chitosan ,Lignosulfonate ,Gold nanoparticles ,Cancer ,MTT assay ,Chemistry ,QD1-999 - Abstract
This work introduces the preparation of a novel type of crosslinked polymers support based on calcium lignosulfonate-chitosan (CLS-CS) hydrogel with capping/reducing ability to encapsulated gold nanoparticles (CLS-CS/Au NPs). The morphology, structure and physicochemical properties of the prepared nanoparticles were characterized by different analytical techniques such as FT-IR, FE-SEM, TEM, EDX-elemental mapping study. The obtained results shown that CLS-CS/Au NPs prepared as spherical particles with sizes of 20–30 nm. The CLS-CS/Au NPs was applied as well for reduction of nitroarenes. The reduction of nitroarenes was obtained with good to high yields within short times. The related nanocatalyst was recovered for eight runs without significant loss of its catalytic performance. The MTT examination was conducted to check the anti-pancreatic cancer, anti-gastric cancer, anti-colorectal carcinoma, and cytotoxicity efficacies of the treated cells with CLS-CS/Au NPs over a 48-hour period on normal (HUVEC) and cancer cells. The IC50 values of the CLS-CS/Au NPs were found to be 169 µg/mL for HT-29 (colorectal carcinoma cell), 196 µg/mL for MKN45 (Gastric cancer cell), and 112 µg/mL for PANC-1 (Pancreatic cancer cell). The growth of malignant colorectal, gastric and pancreatic cells was found to decrease in a dose-dependent manner when exposed to CLS-CS/Au NPs. Following clinical research, CLS-CS/Au NPs shows promise as an effective treatment for colorectal, gastric and pancreatic cancers.
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- 2024
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49. Dietary rumen-protected L-arginine or N-carbamylglutamate enhances placental amino acid transport and suppresses angiogenesis and steroid anabolism in underfed pregnant ewes
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Hao Zhang, Xia Zha, Bei Zhang, Yi Zheng, Xiaoyun Liu, Mabrouk Elsabagh, Yi Ma, Hongrong Wang, Guihua Shu, and Mengzhi Wang
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Angiogenesis factor ,L-arginine ,N-carbamylglutamate ,Placental amino acid transport ,Pregnant ewes ,Steroid anabolism ,Animal culture ,SF1-1100 - Abstract
This study aimed to investigate the effects of dietary supplementation of underfed Hu ewes from d 35 to 110 of gestation with either rumen-protected L-arginine (RP-Arg) or N-carbamylglutamate (NCG) on placental amino acid (AA) transport, angiogenic gene expression, and steroid anabolism. On d 35 of gestation, 32 Hu ewes carrying twin fetuses were randomly divided into four treatment groups, each consisting of eight ewes, and were fed the following diets: A diet providing 100% of NRC's nutrient requirements for pregnant ewes (CON); A diet providing 50% of NRC's nutrient requirements for pregnant ewes (RES); RES diet plus 5 g/d NCG (RES + NCG); or RES diet plus 20 g/d RP-Arg (RES + ARG). On the d 110 of pregnancy, blood samples were taken from the mother, and samples were collected from type A cotyledons (COT; the fetal portions of the placenta). The levels of 17β-estradiol and progesterone in the maternal serum and both the capillary area density (CAD) and capillary surface density (CSD) in type A COT were decreased in response to Arg or NCG supplementation when compared to the RES group. The concentrations of arginine, leucine, putrescine and spermidine in type A COT were higher (P
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- 2023
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50. Influence Mechanism of Cathode Curvature Radius on Corona Discharge at Microscale
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Jingyuan Zhang, Bei Zhang, Yong Yang, Zhenzu Liu, and Hongguang Pan
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micro-corona devices ,corona discharge ,cathode curvature radius ,ionization process ,Technology - Abstract
Micro-corona devices could be extensively utilized in gas sensing, switchgear, biomedicine, and other fields. As the influence mechanism of the cathode curvature radius on micro-corona discharge dynamical processes is very important for performance optimization and the promotion of these devices, a micro-scale corona discharge gas model in a mixture of N2-O2 is proposed based on the fluid–chemical mixing method, which describes the dynamic process of the discharge at atmosphere and normal temperatures. To reveal the influence mechanism of the nanowire curvature radius on the micro-corona discharge, the effect of the cathode nanowire radius on the discharge current, electric field, ionization reaction rate, and charged particle characteristics at different gaps and voltages were determined. The findings indicate that the effect of curvature radius on discharge intensity varies under different gap and voltage conditions. Further analysis indicates that an increase in curvature radius reduces the electric field near the tip while increasing the ionization area and secondary emission area as well as the number of positive ions in the space, consequently affecting the coupling process between the collision ionization and the secondary emission. Especially under the conditions of either small gap or low voltage, a suitable increase in the curvature radius could promote the coupling process and then increase the discharge current.
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- 2024
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