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Chromosomal translocations in wheat derived from alien species are a valuable source of genetic diversity that have provided increases in resistance to various diseases and improved tolerance to abiotic stresses in wheat. These alien genomic segments can also affect multiple traits, with a concomitant ability to alter yield potential in either a positive or negative fashion. The aim of this work was to characterize the effects on yield of two types of translocations, namely T4-derived translocations from Thinopyrum ponticum, carrying the leaf rust resistance gene Lr19, and the TC14 translocation from Th. intermedium, carrying the barley yellow dwarf virus resistance gene Bdv2, in Australian adapted genetic backgrounds and under Australian conditions. A large range of germplasm was developed by crossing donor sources of the translocations into 24 Australian adapted varieties producing 340 genotypes. Yield trials were conducted in 14 environments to identify effects on yield and yield components. The T4 translocations had a positive effect on yield in one high yielding environment, but negatively affected yield in low-yielding environments. The TC14 translocation was generally benign, however, it was associated with a negative impact on yield and reduced height in two genetic backgrounds. The translocation was also associated with a delayed maturity in several backgrounds. The T4 translocations results were consistent with previously published data, whilst this is the first time that such an investigation has been undertaken on the TC14 translocation. Our data suggests a limited role for each of these translocations in Australia. The T4 translocations may be useful in high yielding environments, such as under irrigation in NSW and in the more productive high rainfall regions of south-eastern Australia. Traits associated with the TC14 translocation, such as BYDV resistance and delayed maturity, would make this translocation useful in BYDV-prone areas that experience a less pronounced terminal drought (e.g., south-eastern Australia).

The reaction of winter and spring wheat to infection with barley yellow dwarf virus (BYDV-PAV) was evaluated in three-year small-plot field trials on 71 wheat varieties registered in the Czech Republic and at two locations for two years on 63 selected potential sources of resistance. Disease symptoms (VSS) were visually recorded using a 0-9 scale and the percent reduction of grain weight per spike (GWS-R) was measured on twenty plants per plot. The evaluation showed that among the registered varieties of winter and spring wheat no variety had a high resistance to BYDV (with VSS lower than 3.5). GWS-R ranged between 24% and 60%. Higher variability in VSS was detected for the registered varieties of spring wheat compared to winter wheat. Among the registered varieties of winter wheat, Saskia, Rialto, Meritto, Rexia, and Svitava, as well as the spring wheat Leguan, received the best long-term evaluations. The highest level of resistance was detected for the PSR 3628 line (a hybrid of wheat and couch-grass), but in connection with a low agronomic value. The WKL91-138 line of spring wheat and some varieties (lines) with the detected moderate level of resistance, in particular, could offer good prospects for use in breeding. The presence of the Bdv2 gene was expressed only in the reduction of virus content on the 11th day after inoculation. Nevertheless, genotypes carrying this gene were evaluated in field trials as susceptible or very susceptible to infection with the Czech PAV isolate. Similarly, the presence of the Bdv1 gene detected with the help of WMS130 marker was no assurance of an increased level of resistance to BYDV. Hybridological analyses of crosses with the WKL91-138 line showed a polygenic nature of inheritance. Thus, the marker-assisted selection does not obviously promise success without a focus on detecting a larger number of QTLs.

Barley yellow dwarf virus (BYDV) caused one of the most serious virus diseases of wheat ( Triticum aestivum) worldwide. The wheat- Thinopyrum intermedium translocation line YW642 carries BYDV resistance gene Bdv2. To explore resistant wheat resistome in response to BYDV infection, we used Affymetrix GeneChip® Wheat Genome Arrays to analyze transcriptomes of YW642 and its susceptible parent Zhong8601 at 12 and 72 h postinoculation with BYDV compared to mock-inoculated controls. Relative to mock-inoculated control, 335 defense-related transcripts were upregulated in BYDV-inoculated YW642, among which 70 were upregulated only in BYDV-inoculated YW642 but not in BYDV-inoculated Zhong8601 through clustering analysis. Additional analysis using BYDV-inoculated Zhong8601 as reference revealed that 59 defense-related transcripts were upregulated in BYDV-inoculated YW642. Comparison of these upregulated defense transcripts obtained via the two analysis ways indicated that 19 overlapped defense-related transcripts were highly expressed in BYDV-inoculated YW642 relative to BYDV-inoculated Zhong8601 and mock-inoculated YW642, which likely are significant factors in Bdv2-mediated defense response to BYDV. High expression of some resistance homologous genes, pathogen-associated molecular pattern-triggered immunity-related genes, ABC transporter genes, pathogenesis-related protein genes, and genes in reactive oxygen species, phospholipid signaling, and jasmonic acid-signaling may contribute to Bdv2-mediated defense response to BYDV. [ABSTRACT FROM AUTHOR]