Search

Your search keyword '"Bayne, E."' showing total 164 results
Start Over Author "Bayne, E."
164 results on '"Bayne, E."'

5. Visual spectrogram scanning paired with an observation-confirmation occupancy model improves the efficiency and accuracy of bioacoustic anuran data

Author

Cameron, J., Crosby, A., Paszkowski, C., and Bayne, E.

Subjects
Spectrograph -- Usage, Observational studies -- Usage, Animal vocalization -- Research, Frogs -- Behavior, Zoological research -- Methods, Bioacoustics -- Research, Zoology and wildlife conservation
Abstract

Passive acoustic monitoring using autonomous recording units has improved anuran amphibian call survey data collection. A challenge associated with this approach is the time required for audio data processing. Our objective was to develop a more efficient method of processing and analyzing acoustic data through visual spectrogram scanning and the application of an observation-confirmation occupancy model. We compared detection rates between methods of standard recording listening and visually scanning spectrogram images using different spectrogram parameters. Relative to listening, we found that 1 min spectrograms in two 30 s frames yield the best time efficiency-accuracy trade-off. A standard occupancy model applied to visual scanning data underestimated occupancy estimates relative to listening data for three species and overestimated occupancy for one species. The observation-confirmation model used a subset of listening data to improve the estimates of detection probability from visual scanning and therefore reduced bias in occupancy estimates when compared with using visual scanning data alone. Overall, the combination of the visual scanning method and the observation-confirmation model allowed us to maintain the accuracy of occupancy estimates while greatly increasing the efficiency of anuran data processing. These methods are widely applicable and can increase sample size and precision for acoustic monitoring programs using autonomous recording units. Key words: Boreal Chorus Frog, Pseudacris metadata, Wood Frog, Lithobates sylvaUcus=Rana sylvaUca, Canadian Toad, Bufo hemiophrys=Anaxyrus hemiophrys, Western Toad, Anaxyrus boreas, spectrogram, autonomous recording unit, acoustic processing, passive monitoring. La surveillance acoustique passive a l'aide d'enregistreurs autonomes a ameliore la collecte de donnees dans les releves de cris d'amphibiens anoures. Le temps requis pour traiter les donnees acoustiques est un des defis associes a cette approche. Notre objectif consistait a mettre au point une methode efficace de traitement et d'analyse de donnees acoustiques faisant appel au balayage visuel de spectrogrammes et a l'application d'un modele d'occupation reposant sur la confirmation d'observations. Nous avons compare les taux de detection entre des methodes standards d'ecoute d'enregistrements et de balayage visuel d'images de spectrogrammes utilisant differents parametres de spectrogrammes. Par rapport a l'ecoute, nous avons constate que des spectrogrammes d'une minute divises en deux trames de 30 s offrent le meilleur compromis entre l'efficacite temporelle et Inexactitude. Un modele d'occupation standard applique a des donnees de balayage visuel a sous-estime l'occupation par rapport aux donnees d'ecoute pour trois especes et l'a surestimee pour une espece. Un sous-ensemble de donnees d'ecoute a ete utilise dans le modele de confirmation d'observations pour ameliorer les estimations de la probability de detection par balayage visuel, reduisant ainsi le biais des estimations d'occupation comparativement aux estimations reposant sur les seules donnees de balayage visuel. Globalement, la combinaison de la methode de balayage visuel et du modele de confirmation d'observations nous a permis de maintenir l'exactitude des estimations d'occupation tout en rehaussant considerablement l'efficacite du traitement de donnees relatives aux anoures. Ces methodes ont de nombreuses applications et peuvent accroitre la taille des echantillons et la precision des programmes de surveillance acoustiques faisant appel a des enregistreurs de donnees autonomes. [Traduit par la Redaction] Mots-cles : rainette faux-grillon boreale, Pseudacris maculata, grenouille des bois, Lithobates sylvaticus = Rana sylvatica, crapaud du Canada, Bufo hemiophrys = Anaxyrus hemiophrys, crapaud de l'Ouest, Anaxyrus boreas, spectrogramme, enregistreur de donnees autonome, traitement de donnees acoustiques, surveillance passive., Introduction Advances in bioacoustic technology now allow for widespread passive monitoring of vocal and non-vocal taxa, including anuran amphibians, birds, bats, canids, and insects (Sugai et al. 2019; Garland et [...]

Published
2020
Full Text
View/download PDF

10. Effects of natural resource development on the terrestrial biodiversity of Canadian boreal forests

Author

Venier, L.A., Thompson, I.D., Fleming, R., Malcolm, J., Aubin, I., Trofymow, J.A., Langor, D., Sturrock, R., Patry, C., Outerbridge, R.O., Holmes, S.B., Haeussler, S., De Grandpre, L., Chen, H.Y.H., Bayne, E., Arsenault, A., and Brandt, J.P.

Subjects
Biological diversity -- Analysis, Economic development -- Analysis -- Canada, Taigas -- Research -- Environmental aspects, Forest management -- Methods -- Analysis, Environmental issues
Abstract

Much of Canada's terrestrial biodiversity is supported by boreal forests. Natural resource development in boreal forests poses risks to this biodiversity. This paper reviews the scientific literature to assess the effects of natural resource development on terrestrial biodiversity in Canadian boreal forests. We address four questions: (1) To what extent have Canadian boreal forests changed due to natural resource development? (2) How has biodiversity responded to these changes? (3) Will the biodiversity of second-growth forests converge with that of primary boreal forests? (4) Are we losing species from boreal forests? We focus on trees, understory plants, insects, fungi, selected mammals, and songbirds because these groups have been most studied. We review more than 600 studies and found that changes in community composition are prevalent in response to large-scale conversion of forest types, changes in stand structures and age distributions, and altered landscape structure resulting from forest management and habitat loss associated with other developments such as oil and gas, hydroelectric, and mining. The southern boreal forest has been more highly impacted than the north due to more extensive forest management and the cumulative effects of multiple forms of development. There is abundant evidence that most species are not in danger of being extirpated from the boreal forest due to these anthropogenic changes. A few species, including woodland caribou (Rangifer tarandus) and grizzly bear (Ursus arctos), have, however, undergone long-term range contractions. Significant gaps in our ability to assess the effects of natural resource development on biodiversity in the boreal zone are the lack of long-term spatial and population data to monitor the impact of forest changes on ecosystems and species. Key words: terrestrial biodiversity, boreal forest, natural resource development, forest management, forest conversion. Une bonne partie de la biodiversite du Canada se retrouve en foret boreale. Le developpement des ressources naturelles dans des forets boreales presente des risques pour cette biodiversite. Les auteurs presentent une revue de la litterature pour evaluer les effets du developpement des ressources naturelles sur la biodiversite terrestre dans les forets boreales canadiennes. Ils ont souleve quatre questions: (1) jusqu'il quel point les forets boreales canadiennes se sont vues modifiees par le developpement des ressources naturelles ? (2) Comment la biodiversite a-t-elle reagi a ces changements ? (3) Y aura-t-il convergence de la biodiversite des forets de seconde venue avec celle des forets boreales primaires ? (4) Subissons-nous des pertes d'especes en forets boreales ? Les auteurs se sont interesses en particulier aux arbres, aux plantes de sous-bois, aux insectes, aux champignons, a des mammiferes et oiseaux chanteurs selectionnes, car ces groupes ont ete les plus etudies. Ils ont suivi plus de 600 especes et ont constate que les changements de composition prevalent en reaction a des modifications a grande echelle des types forestiers, des changements de la structure, des classes d'age, des modifications aux structures des paysages resultant de l'amenagement forestier, ainsi que des pertes d'habitat associees avec d'autres developpements comme l'huile, le gaz, l'hydroelectricite et les mines. La partie meridionale de la foret boreale a recu plus d'impacts que la partie nordique suite a des amenagements forestiers plus intensifs et aux effets cumulatifs des diverses formes de developpement. Il y a beaucoup de preuves que la plupart des especes ne se retrouvent pas en danger d'extinction en forets boreales sous l'influence de modifications anthropogenes. Quelques especes, incluant le caribou forestier (Rangifer tarandus) et l'ours grizzly (Ursus arctos) ont cependant subi des contractions d'habitat a long terme. On observe des deficiences significatives dans notre capacite a evaluer les effets du developpement des ressources naturelles sur la biodiversite dans la zone boreale, du a l'absence de donnees spatiales et a long terme sur les populations, pour suivre l'impact des modifications forestieres sur les ecosystemes et les especes. [Traduit par la Redaction] Mots-cles: biodiversite terrestre, foret boreale, developpement des ressources naturelles, amenagement forestier, conversion des forets., 1. Introduction Biodiversity, broadly defined as the variety of life and its processes, including genes, species, communities, and ecosystems and the ecological and evolutionary processes that keep them functioning (Noss [...]

Published
2014
Full Text
View/download PDF

12. The Knowledge Base for Interns' Teaching Decisions. Monograph No. 15.

Author

Saskatchewan Univ., Saskatoon. Coll. of Education., Johnston, Janet M., and Bayne, E. Lynn

Abstract

A study of post degree elementary interns at the University of Saskatchewan (Canada) (N=25) was conducted to determine the main sources of information they employed in decision making related to teaching and relationships in the school. The study focused on: (1) the extent to which interns used the knowledge base gained from education classes in planning decisions for teaching and while teaching; and (2) what other sources of knowledge influenced their decision making. Data were collected by conducting a structured interview with students and doing a similar interview with cooperating teachers as a check on how much knowledge they had given the interns. The interns were asked to rate the education courses taken in terms of usable knowledge they had gained from the classes. Cooperating teachers and interns were questioned on initial perceptions of the internship prior to classroom work actually beginning. These data were collected to determine the correlation between the preconceived notions of internship and reality. Also, each intern was videotaped for two lessons, one in mathematics and one in language arts. The tapes were analyzed for certain teacher behaviors and correlations were determined between teaching scores, perceptions, and the origins of the knowledge base. (Author/LL)

Published
1992

13. Reflection and Collaboration in the Practicum: The Mayfair Project. Monograph No. 14.

Author

Saskatchewan Univ., Saskatoon. Coll. of Education., Barnett, Don C., and Bayne, E. Lynn

Abstract

This paper describes a pilot project in which interns were clustered in an elementary school in Saskatchewan (Canada) to work in collaboration with the teaching staff toward school goals as well as internship goals. The role of the college supervisor was primarily one of supporter, problem-solver, resource provider, and idea stimulator. The working relationship between teacher and intern moved through three phases focusing on: (1) specific feedback based on technical data collection; (2) exploration of ideas and alternative approaches; and (3) interpretation and reflection. Reflective journal writing focusing on descriptive, analytical, and affective levels of reflection was on-going throughout the internship. Feedback indicated a positive perspective on the experience by both interns and teachers. Some adjustments for the three days of inservices were recommended, positive feelings for peer support and peer coaching ranged from very little to substantial, and suggestions for changes in another school undertaking a similar project were provided. The project concluded that a successful practicum is enhanced if the process is perceived within the framework of the total school program and a paradigm of collaboration and experimentation is established at the outset. (Author/LL)

Published
1992

21. The database of the PREDICTS (Projecting Responses of Ecological Diversity In Changing Terrestrial Systems) project

Author

Hudson, L., Newbold, T., Contu, S., Hill, S., Lysenko, I., De Palma, A., Phillips, H., Alhusseini, T., Bedford, F., Bennett, D., Booth, H., Burton, V., Chng, C., Choimes, A., Correia, D., Day, J., Echeverria-Londono, S., Emerson, S., Gao, D., Garon, M., Harrison, M., Ingram, D., Jung, M., Kemp, V., Kirkpatrick, L., Martin, C., Pan, Y., Pask-Hale, G., Pynegar, E., Robinson, A., Sanchez-Ortiz, K., Senior, R., Simmons, B., White, H., Zhang, H., Aben, J., Abrahamczyk, S., Adum, G., Aguilar-Barquero, V., Aizen, M., Albertos, B., Alcala, E., del Mar Alguacil, M., Alignier, A., Ancrenaz, M., Andersen, A., Arbelaez-Cortes, E., Armbrecht, I., Arroyo-Rodriguez, V., Aumann, T., Axmacher, J., Azhar, B., Azpiroz, A., Baeten, L., Bakayoko, A., Baldi, A., Banks, J., Baral, S., Barlow, J., Barratt, B., Barrico, L., Bartolommei, P., Barton, D., Basset, Y., Batary, P., Bates, A., Baur, B., Bayne, E., Beja, P., Benedick, S., Berg, A., Bernard, H., Berry, N., Bhatt, D., Bicknell, J., Bihn, J., Blake, R., Bobo, K., Bocon, R., Boekhout, T., Bohning-Gaese, K., Bonham, K., Borges, P., Borges, S., Boutin, C., Bouyer, J., Bragagnolo, C., Brandt, J., Brearley, F., Brito, I., Bros, V., Brunet, J., Buczkowski, G., Buddle, C., Bugter, R., Buscardo, E., Buse, J., Cabra-Garcia, J., Caceres, N., Cagle, N., Calvino-Cancela, M., Cameron, S., Cancello, E., Caparros, R., Cardoso, P., Carpenter, D., Carrijo, T., Carvalho, A., Cassano, C., Castro, H., Castro-Luna, A., Cerda, R., Cerezo, A., Chapman, K., Chauvat, M., Christensen, M., Clarke, F., Cleary, D., Colombo, G., Connop, S., Craig, M., Cruz-Lopez, L., Cunningham, S., D'Aniello, B., D'Cruze, N., da Silva, P., Dallimer, M., Danquah, E., Darvill, B., Dauber, J., Davis, A., Dawson, J., de Sassi, C., de Thoisy, B., Deheuvels, O., Dejean, A., Devineau, J., Diekoetter, T., Dolia, J., Dominguez, E., Dominguez-Haydar, Y., Dorn, S., Draper, I., Dreber, N., Dumont, B., Dures, S., Dynesius, M., Edenius, L., Eggleton, P., Eigenbrod, F., Elek, Z., Entling, M., Esler, K., De Lima, R., Faruk, A., Farwig, N., Fayle, T., Felicioli, A., Felton, A., Fensham, R., Fernandez, I., Ferreira, C., Ficetola, G., Fiera, C., Filgueiras, B., Firincioglu, H., Flaspohler, D., Floren, A., Fonte, S., Fournier, A., Fowler, R., Franzen, M., Fraser, L., Fredriksson, G., Freire-, G., Frizzo, T., Fukuda, D., Furlani, D., Gaigher, R., Ganzhorn, J., Garcia, K., Garcia-R, J., Garden, J., Garilleti, R., Ge, B., Gendreau-Berthiaume, B., Gerard, P., Gheler-Costa, C., Gilbert, B., Giordani, P., Giordano, S., Golodets, C., Gomes, L., Gould, R., Goulson, D., Gove, Aaron, Granjon, L., Grass, I., Gray, C., Grogan, J., Gu, W., Guardiola, M., Gunawardene, Nihara, Gutierrez, A., Gutierrez-Lamus, D., Haarmeyer, D., Hanley, M., Hanson, T., Hashim, N., Hassan, S., Hatfield, R., Hawes, J., Hayward, M., Hebert, C., Helden, A., Henden, J., Henschel, P., Hernandez, L., Herrera, J., Herrmann, F., Herzog, F., Higuera-Diaz, D., Hilje, B., Hofer, H., Hoffmann, A., Horgan, F., Hornung, E., Horvath, R., Hylander, K., Isaacs-Cubides, P., Ishida, H., Ishitani, M., Jacobs, C., Jaramillo, V., Jauker, B., Jimenez Hernandez, F., Johnson, M., Jolli, V., Jonsell, M., Juliani, S., Jung, T., Kapoor, V., Kappes, H., Kati, V., Katovai, E., Kellner, K., Kessler, M., Kirby, K., Kittle, A., Knight, M., Knop, E., Kohler, F., Koivula, M., Kolb, A., Kone, M., Koroesi, A., Krauss, J., Kumar, A., Kumar, R., Kurz, D., Kutt, A., Lachat, T., Lantschner, V., Lara, F., Lasky, J., Latta, S., Laurance, W., Lavelle, P., Le Feon, V., LeBuhn, G., Legare, J., Lehouck, V., Lencinas, M., Lentini, P., Letcher, S., Li, Q., Litchwark, S., Littlewood, N., Liu, Y., Lo-Man-Hung, N., Lopez-Quintero, C., Louhaichi, M., Lovei, G., Lucas-Borja, M., Luja, V., Luskin, M., MacSwiney G, M., Maeto, K., Magura, T., Mallari, N., Malone, L., Malonza, P., Malumbres-Olarte, J., Mandujano, S., Maren, I., Marin-Spiotta, E., Marsh, C., Marshall, E., Martinez, E., Pastur, G., Mateos, D., Mayfield, M., Mazimpaka, V., McCarthy, J., McCarthy, K., McFrederick, Q., McNamara, S., Medina, N., Medina, R., Mena, J., Mico, E., Mikusinski, G., Milder, J., Miller, J., Miranda-Esquivel, D., Moir, M., Morales, C., Muchane, M., Mudri-Stojnic, S., Munira, A., Muonz-Alonso, A., Munyekenye, B., Naidoo, R., Naithani, A., Nakagawa, M., Nakamura, A., Nakashima, Y., Naoe, S., Nates-Parra, G., Gutierrez, D., Navarro-Iriarte, L., Ndang'ang'a, P., Neuschulz, E., Ngai, J., Nicolas, V., Nilsson, S., Noreika, N., Norfolk, O., Noriega, J., Norton, D., Noeske, N., Nowakowski, A., Numa, C., O'Dea, N., O'Farrell, P., Oduro, W., Oertli, S., Ofori-Boateng, C., Oke, C., Oostra, V., Osgathorpe, L., Eduardo Otavo, S., Page, N., Paritsis, J., Parra-H, A., Parry, L., Pe'er, G., Pearman, P., Pelegrin, N., Pelissier, R., Peres, C., Peri, P., Persson, A., Petanidou, T., Peters, M., Pethiyagoda, R., Phalan, B., Philips, T., Pillsbury, F., Pincheira-Ulbrich, J., Pineda, E., Pino, J., Pizarro-Araya, J., Plumptre, A., Poggio, S., Politi, N., Pons, P., Poveda, K., Power, E., Presley, S., Proenca, V., Quaranta, M., Quintero, C., Rader, R., Ramesh, B., Ramirez-Pinilla, M., Ranganathan, J., Rasmussen, C., Redpath-Downing, N., Reid, J., Reis, Y., Rey Benayas, J., Carlos Rey-Velasco, J., Reynolds, C., Ribeiro, D., Richards, M., Richardson, B., Richardson, M., Macip Rios, R., Robinson, R., Robles, C., Roembke, J., Romero-Duque, L., Ros, M., Rosselli, L., Rossiter, S., Roth, D., Roulston, T., Rousseau, L., Rubio, A., Ruel, J., Sadler, J., Safian, S., Saldana-Vazquez, R., Sam, K., Samnegard, U., Santana, J., Santos, X., Savage, J., Schellhorn, N., Schilthuizen, M., Schmiedel, U., Schmitt, C., Schon, N., Schuepp, C., Schumann, K., Schweiger, O., Scott, D., Scott, K., Sedlock, J., Seefeldt, S., Shahabuddin, G., Shannon, G., Sheil, D., Sheldon, F., Shochat, E., Siebert, S., Silva, F., Simonetti, J., Slade, E., Smith, J., Smith-Pardo, A., Sodhi, N., Somarriba, E., Sosa, R., Soto Quiroga, G., St-Laurent, M., Starzomski, B., Stefanescu, C., Steffan-Dewenter, I., Stouffer, P., Stout, J., Strauch, A., Struebig, M., Su, Z., Suarez-Rubio, M., Sugiura, S., Summerville, K., Sung, Y., Sutrisno, H., Svenning, J., Teder, T., Threlfall, C., Tiitsaar, A., Todd, J., Tonietto, R., Torre, I., Tothmeresz, B., Tscharntke, T., Turner, E., Tylianakis, J., Uehara-Prado, M., Urbina-Cardona, N., Vallan, D., Vanbergen, A., Vasconcelos, H., Vassilev, K., Verboven, H., Verdasca, M., Verdu, J., Vergara, C., Vergara, P., Verhulst, J., Virgilio, M., Van Vu, L., Waite, E., Walker, T., Wang, H., Wang, Y., Watling, J., Weller, B., Wells, K., Westphal, C., Wiafe, E., Williams, C., Willig, M., Woinarski, J., Wolf, J., Wolters, V., Woodcock, B., Wu, J., Wunderle, J., Yamaura, Y., Yoshikura, S., Yu, D., Zaitsev, A., Zeidler, J., Zou, F., Collen, B., Ewers, R., Mace, G., Purves, D., Scharlemann, J., Purvis, A., Hudson, L., Newbold, T., Contu, S., Hill, S., Lysenko, I., De Palma, A., Phillips, H., Alhusseini, T., Bedford, F., Bennett, D., Booth, H., Burton, V., Chng, C., Choimes, A., Correia, D., Day, J., Echeverria-Londono, S., Emerson, S., Gao, D., Garon, M., Harrison, M., Ingram, D., Jung, M., Kemp, V., Kirkpatrick, L., Martin, C., Pan, Y., Pask-Hale, G., Pynegar, E., Robinson, A., Sanchez-Ortiz, K., Senior, R., Simmons, B., White, H., Zhang, H., Aben, J., Abrahamczyk, S., Adum, G., Aguilar-Barquero, V., Aizen, M., Albertos, B., Alcala, E., del Mar Alguacil, M., Alignier, A., Ancrenaz, M., Andersen, A., Arbelaez-Cortes, E., Armbrecht, I., Arroyo-Rodriguez, V., Aumann, T., Axmacher, J., Azhar, B., Azpiroz, A., Baeten, L., Bakayoko, A., Baldi, A., Banks, J., Baral, S., Barlow, J., Barratt, B., Barrico, L., Bartolommei, P., Barton, D., Basset, Y., Batary, P., Bates, A., Baur, B., Bayne, E., Beja, P., Benedick, S., Berg, A., Bernard, H., Berry, N., Bhatt, D., Bicknell, J., Bihn, J., Blake, R., Bobo, K., Bocon, R., Boekhout, T., Bohning-Gaese, K., Bonham, K., Borges, P., Borges, S., Boutin, C., Bouyer, J., Bragagnolo, C., Brandt, J., Brearley, F., Brito, I., Bros, V., Brunet, J., Buczkowski, G., Buddle, C., Bugter, R., Buscardo, E., Buse, J., Cabra-Garcia, J., Caceres, N., Cagle, N., Calvino-Cancela, M., Cameron, S., Cancello, E., Caparros, R., Cardoso, P., Carpenter, D., Carrijo, T., Carvalho, A., Cassano, C., Castro, H., Castro-Luna, A., Cerda, R., Cerezo, A., Chapman, K., Chauvat, M., Christensen, M., Clarke, F., Cleary, D., Colombo, G., Connop, S., Craig, M., Cruz-Lopez, L., Cunningham, S., D'Aniello, B., D'Cruze, N., da Silva, P., Dallimer, M., Danquah, E., Darvill, B., Dauber, J., Davis, A., Dawson, J., de Sassi, C., de Thoisy, B., Deheuvels, O., Dejean, A., Devineau, J., Diekoetter, T., Dolia, J., Dominguez, E., Dominguez-Haydar, Y., Dorn, S., Draper, I., Dreber, N., Dumont, B., Dures, S., Dynesius, M., Edenius, L., Eggleton, P., Eigenbrod, F., Elek, Z., Entling, M., Esler, K., De Lima, R., Faruk, A., Farwig, N., Fayle, T., Felicioli, A., Felton, A., Fensham, R., Fernandez, I., Ferreira, C., Ficetola, G., Fiera, C., Filgueiras, B., Firincioglu, H., Flaspohler, D., Floren, A., Fonte, S., Fournier, A., Fowler, R., Franzen, M., Fraser, L., Fredriksson, G., Freire-, G., Frizzo, T., Fukuda, D., Furlani, D., Gaigher, R., Ganzhorn, J., Garcia, K., Garcia-R, J., Garden, J., Garilleti, R., Ge, B., Gendreau-Berthiaume, B., Gerard, P., Gheler-Costa, C., Gilbert, B., Giordani, P., Giordano, S., Golodets, C., Gomes, L., Gould, R., Goulson, D., Gove, Aaron, Granjon, L., Grass, I., Gray, C., Grogan, J., Gu, W., Guardiola, M., Gunawardene, Nihara, Gutierrez, A., Gutierrez-Lamus, D., Haarmeyer, D., Hanley, M., Hanson, T., Hashim, N., Hassan, S., Hatfield, R., Hawes, J., Hayward, M., Hebert, C., Helden, A., Henden, J., Henschel, P., Hernandez, L., Herrera, J., Herrmann, F., Herzog, F., Higuera-Diaz, D., Hilje, B., Hofer, H., Hoffmann, A., Horgan, F., Hornung, E., Horvath, R., Hylander, K., Isaacs-Cubides, P., Ishida, H., Ishitani, M., Jacobs, C., Jaramillo, V., Jauker, B., Jimenez Hernandez, F., Johnson, M., Jolli, V., Jonsell, M., Juliani, S., Jung, T., Kapoor, V., Kappes, H., Kati, V., Katovai, E., Kellner, K., Kessler, M., Kirby, K., Kittle, A., Knight, M., Knop, E., Kohler, F., Koivula, M., Kolb, A., Kone, M., Koroesi, A., Krauss, J., Kumar, A., Kumar, R., Kurz, D., Kutt, A., Lachat, T., Lantschner, V., Lara, F., Lasky, J., Latta, S., Laurance, W., Lavelle, P., Le Feon, V., LeBuhn, G., Legare, J., Lehouck, V., Lencinas, M., Lentini, P., Letcher, S., Li, Q., Litchwark, S., Littlewood, N., Liu, Y., Lo-Man-Hung, N., Lopez-Quintero, C., Louhaichi, M., Lovei, G., Lucas-Borja, M., Luja, V., Luskin, M., MacSwiney G, M., Maeto, K., Magura, T., Mallari, N., Malone, L., Malonza, P., Malumbres-Olarte, J., Mandujano, S., Maren, I., Marin-Spiotta, E., Marsh, C., Marshall, E., Martinez, E., Pastur, G., Mateos, D., Mayfield, M., Mazimpaka, V., McCarthy, J., McCarthy, K., McFrederick, Q., McNamara, S., Medina, N., Medina, R., Mena, J., Mico, E., Mikusinski, G., Milder, J., Miller, J., Miranda-Esquivel, D., Moir, M., Morales, C., Muchane, M., Mudri-Stojnic, S., Munira, A., Muonz-Alonso, A., Munyekenye, B., Naidoo, R., Naithani, A., Nakagawa, M., Nakamura, A., Nakashima, Y., Naoe, S., Nates-Parra, G., Gutierrez, D., Navarro-Iriarte, L., Ndang'ang'a, P., Neuschulz, E., Ngai, J., Nicolas, V., Nilsson, S., Noreika, N., Norfolk, O., Noriega, J., Norton, D., Noeske, N., Nowakowski, A., Numa, C., O'Dea, N., O'Farrell, P., Oduro, W., Oertli, S., Ofori-Boateng, C., Oke, C., Oostra, V., Osgathorpe, L., Eduardo Otavo, S., Page, N., Paritsis, J., Parra-H, A., Parry, L., Pe'er, G., Pearman, P., Pelegrin, N., Pelissier, R., Peres, C., Peri, P., Persson, A., Petanidou, T., Peters, M., Pethiyagoda, R., Phalan, B., Philips, T., Pillsbury, F., Pincheira-Ulbrich, J., Pineda, E., Pino, J., Pizarro-Araya, J., Plumptre, A., Poggio, S., Politi, N., Pons, P., Poveda, K., Power, E., Presley, S., Proenca, V., Quaranta, M., Quintero, C., Rader, R., Ramesh, B., Ramirez-Pinilla, M., Ranganathan, J., Rasmussen, C., Redpath-Downing, N., Reid, J., Reis, Y., Rey Benayas, J., Carlos Rey-Velasco, J., Reynolds, C., Ribeiro, D., Richards, M., Richardson, B., Richardson, M., Macip Rios, R., Robinson, R., Robles, C., Roembke, J., Romero-Duque, L., Ros, M., Rosselli, L., Rossiter, S., Roth, D., Roulston, T., Rousseau, L., Rubio, A., Ruel, J., Sadler, J., Safian, S., Saldana-Vazquez, R., Sam, K., Samnegard, U., Santana, J., Santos, X., Savage, J., Schellhorn, N., Schilthuizen, M., Schmiedel, U., Schmitt, C., Schon, N., Schuepp, C., Schumann, K., Schweiger, O., Scott, D., Scott, K., Sedlock, J., Seefeldt, S., Shahabuddin, G., Shannon, G., Sheil, D., Sheldon, F., Shochat, E., Siebert, S., Silva, F., Simonetti, J., Slade, E., Smith, J., Smith-Pardo, A., Sodhi, N., Somarriba, E., Sosa, R., Soto Quiroga, G., St-Laurent, M., Starzomski, B., Stefanescu, C., Steffan-Dewenter, I., Stouffer, P., Stout, J., Strauch, A., Struebig, M., Su, Z., Suarez-Rubio, M., Sugiura, S., Summerville, K., Sung, Y., Sutrisno, H., Svenning, J., Teder, T., Threlfall, C., Tiitsaar, A., Todd, J., Tonietto, R., Torre, I., Tothmeresz, B., Tscharntke, T., Turner, E., Tylianakis, J., Uehara-Prado, M., Urbina-Cardona, N., Vallan, D., Vanbergen, A., Vasconcelos, H., Vassilev, K., Verboven, H., Verdasca, M., Verdu, J., Vergara, C., Vergara, P., Verhulst, J., Virgilio, M., Van Vu, L., Waite, E., Walker, T., Wang, H., Wang, Y., Watling, J., Weller, B., Wells, K., Westphal, C., Wiafe, E., Williams, C., Willig, M., Woinarski, J., Wolf, J., Wolters, V., Woodcock, B., Wu, J., Wunderle, J., Yamaura, Y., Yoshikura, S., Yu, D., Zaitsev, A., Zeidler, J., Zou, F., Collen, B., Ewers, R., Mace, G., Purves, D., Scharlemann, J., and Purvis, A.

Abstract

The PREDICTS project—Projecting Responses of Ecological Diversity In Changing Terrestrial Systems (www.predicts.org.uk)—has collated from published studies a large, reasonably representative database of comparable samples of biodiversity from multiple sites that differ in the nature or intensity of human impacts relating to land use. We have used this evidence base to develop global and regional statistical models of how local biodiversity responds to these measures. We describe and make freely available this 2016 release of the database, containing more than 3.2 million records sampled at over 26,000 locations and representing over 47,000 species. We outline how the database can help in answering a range of questions in ecology and conservation biology. To our knowledge, this is the largest and most geographically and taxonomically representative database of spatial comparisons of biodiversity that has been collated to date; it will be useful to researchers and international efforts wishing to model and understand the global status of biodiversity.

Published
2017

22. Configuration Management in Transportation Management Systems: Evaluation of Need

Author

Brian L. Smith and Bayne E. Smith

Subjects
Transportation planning, Configuration management, Operations research, Computer science, Program management, business.industry, Mechanical Engineering, Information technology, computer.software_genre, Advanced Traffic Management System, Engineering management, Systems management, Management system, business, computer, Intelligent transportation system, Civil and Structural Engineering
Abstract

Configuration management, a process developed to control change in complex information technology–based systems, has attracted an increasing amount of attention from the transportation engineering community. As the intelligent transportation systems program accelerates and transportation departments develop and operate increasingly complex transportation management systems, the need for configuration management grows. In this research effort the need for configuration management within transportation management systems is examined by exploring the foundations of configuration management, analyzing the key functions of transportation management, surveying transportation officials, and conducting a case study to examine the configuration management process used by the Georgia Department of Transportation.

Published
2001
Full Text
View/download PDF

25. The PREDICTS database: a global database of how local terrestrial biodiversity responds to human impacts

Author

Hudson, L., Newbold, T., Contu, S., Hill, S., Lysenko, I., De Palma, A., Phillips, H., Senior, R., Bennett, D., Booth, H., Choimes, A., Correia, D., Day, J., Echeverria-Londono, S., Garon, M., Harrison, M., Ingram, D., Jung, M., Kemp, V., Kirkpatrick, L., Martin, C., Pan, Y., White, H., Aben, J., Abrahamczyk, S., Adum, G., Aguilar-Barquero, V., Aizen, M., Ancrenaz, M., Arbelaez-Cortes, E., Armbrecht, I., Azhar, B., Azpiroz, A., Baeten, L., Baldi, A., Banks, J., Barlow, J., Batary, P., Bates, A., Bayne, E., Beja, P., Berg, A., Berry, N., Bicknell, J., Bihn, J., Bohning-Gaese, K., Boekhout, T., Boutin, C., Bouyer, J., Brearley, F., Brito, I., Brunet, J., Buczkowski, G., Buscardo, E., Cabra-Garcia, J., Calvino-Cancela, M., Cameron, S., cancello, E., Carrijo, T., carvalho, A., Castro, H., Castro-Luna, A., Cerda, R., Cerezo, A., Chauvat, M., Clarke, F., Cleary, D., Connop, S., D'Aniello, B., da Silva, P.G., Darvill, B., Dauber, J., Dejean, A., Diekotter, T., Dominguez-Haydar, Y., Dormann, C., Dumont, B., Dures, S., Dynesius, M., Edenius, L., Elek, Z., Entling, M., Farwig, N., Fayle, T., Felicioli, A., Felton, A., Ficetola, G., Filgueiras, B., Fonte, S., Fraser, L., Fukuda, D., Furlani, D., Ganzhorn, J., Garden, J., Gheler-Costa, C., Giordani, P., Giordano, S., Gottschalk, M., Goulson, D., Gove, Aaron, Grogan, J., Hanley, M., Hanson, T., Hashim, N., Hawes, J., Hebert, C., Helden, A., Henden, J., Hernandez, L., Herzog, F., Higuera-Diaz, D., Hilje, B., Horgan, F., Horvath, R., Hylander, K., Issacs-Cubides, P., Ishitani, M., Jacobs, C., Jaramillo, V., Jauker, B., Jonsell, M., Jung, T., Kapoor, V., Kati, V., Katovai, E., Kessler, M., Knop, E., Kolb, A., Korosi, A., Lachat, T., Lantschner, V., Le Feon, V., LeBuhn, G., Legare, J., Letcher, S., Littlewood, N., Lopez--Quintero, C., Louhaichi, M., Lovei, G., Lucas-Borja, M., Luja, V., Maeto, K., Magura, T., Mallari, N., Marin-Spiotta, E., Marshall, E., Martinez, E., Mayfield, M., Mikusinski, G., Milder, J., Miller, J., Morales, C., Muchane, M., Naidoo, R., Nakamura, A., Naoe, S., Nates-Parra, G., Navarrete Gutierrez, D., Neuschulz, E., Noreika, N., Norfolk, O., Noriega, J., Noske, N., O'Dea, N., Oduro, W., Ofori-Boateng, C., Oke, C., Osgathorpe, L., Paritsis, J., Parrah, A., Pelegrin, N., Peres, C., Persson, A., Petanidou, T., Phalan, B., Philips, T.K., Poveda, K., Power, E., Presley, S., Proenca, V., Quaranta, M., Quintero, C., Redpath-Downing, N., Reid, J.L., Reis, Y., Ribeiro, D., Richardson, B., Richardson, M., Robles, C., Rombke, J., Romero-Duque, L.P., Rosselli, L., Rossiter, S., Roulston, T., Rousseau, L., Sadler, J., Safian, S., Saldana-Vazquez, R., Samnegard, U., Schuepp, C., Schweiger, O., Sedlock, J., Shahabuddin, G., Sheil, D., Silva, F., Slade, E., Smith-Pardo, A., Sodhi, N., Somarriba, E., Sosa, R., Stout, J., Struebig, M., Sung, Y., Threlfall, C., Tonietto, R., Tothmeresz, B., Tscharntke, T., Turner, E., Tylianakis, J., Vanbergen, A., Vassilev, K., Verboven, H., Vergara, C., Vergara, P., Verhulst, J., Walker, T., Wang, Y., Watling, J., Wells, K., Williams, C., Willig, M., Woinarski, J., Wolf, J., Woodcock, B., Yu, D., Zaitsev, A., Collen, B., Ewers, R., Mace, G., Purves, D., Scharlemann, J., Purvis, A., Hudson, L., Newbold, T., Contu, S., Hill, S., Lysenko, I., De Palma, A., Phillips, H., Senior, R., Bennett, D., Booth, H., Choimes, A., Correia, D., Day, J., Echeverria-Londono, S., Garon, M., Harrison, M., Ingram, D., Jung, M., Kemp, V., Kirkpatrick, L., Martin, C., Pan, Y., White, H., Aben, J., Abrahamczyk, S., Adum, G., Aguilar-Barquero, V., Aizen, M., Ancrenaz, M., Arbelaez-Cortes, E., Armbrecht, I., Azhar, B., Azpiroz, A., Baeten, L., Baldi, A., Banks, J., Barlow, J., Batary, P., Bates, A., Bayne, E., Beja, P., Berg, A., Berry, N., Bicknell, J., Bihn, J., Bohning-Gaese, K., Boekhout, T., Boutin, C., Bouyer, J., Brearley, F., Brito, I., Brunet, J., Buczkowski, G., Buscardo, E., Cabra-Garcia, J., Calvino-Cancela, M., Cameron, S., cancello, E., Carrijo, T., carvalho, A., Castro, H., Castro-Luna, A., Cerda, R., Cerezo, A., Chauvat, M., Clarke, F., Cleary, D., Connop, S., D'Aniello, B., da Silva, P.G., Darvill, B., Dauber, J., Dejean, A., Diekotter, T., Dominguez-Haydar, Y., Dormann, C., Dumont, B., Dures, S., Dynesius, M., Edenius, L., Elek, Z., Entling, M., Farwig, N., Fayle, T., Felicioli, A., Felton, A., Ficetola, G., Filgueiras, B., Fonte, S., Fraser, L., Fukuda, D., Furlani, D., Ganzhorn, J., Garden, J., Gheler-Costa, C., Giordani, P., Giordano, S., Gottschalk, M., Goulson, D., Gove, Aaron, Grogan, J., Hanley, M., Hanson, T., Hashim, N., Hawes, J., Hebert, C., Helden, A., Henden, J., Hernandez, L., Herzog, F., Higuera-Diaz, D., Hilje, B., Horgan, F., Horvath, R., Hylander, K., Issacs-Cubides, P., Ishitani, M., Jacobs, C., Jaramillo, V., Jauker, B., Jonsell, M., Jung, T., Kapoor, V., Kati, V., Katovai, E., Kessler, M., Knop, E., Kolb, A., Korosi, A., Lachat, T., Lantschner, V., Le Feon, V., LeBuhn, G., Legare, J., Letcher, S., Littlewood, N., Lopez--Quintero, C., Louhaichi, M., Lovei, G., Lucas-Borja, M., Luja, V., Maeto, K., Magura, T., Mallari, N., Marin-Spiotta, E., Marshall, E., Martinez, E., Mayfield, M., Mikusinski, G., Milder, J., Miller, J., Morales, C., Muchane, M., Naidoo, R., Nakamura, A., Naoe, S., Nates-Parra, G., Navarrete Gutierrez, D., Neuschulz, E., Noreika, N., Norfolk, O., Noriega, J., Noske, N., O'Dea, N., Oduro, W., Ofori-Boateng, C., Oke, C., Osgathorpe, L., Paritsis, J., Parrah, A., Pelegrin, N., Peres, C., Persson, A., Petanidou, T., Phalan, B., Philips, T.K., Poveda, K., Power, E., Presley, S., Proenca, V., Quaranta, M., Quintero, C., Redpath-Downing, N., Reid, J.L., Reis, Y., Ribeiro, D., Richardson, B., Richardson, M., Robles, C., Rombke, J., Romero-Duque, L.P., Rosselli, L., Rossiter, S., Roulston, T., Rousseau, L., Sadler, J., Safian, S., Saldana-Vazquez, R., Samnegard, U., Schuepp, C., Schweiger, O., Sedlock, J., Shahabuddin, G., Sheil, D., Silva, F., Slade, E., Smith-Pardo, A., Sodhi, N., Somarriba, E., Sosa, R., Stout, J., Struebig, M., Sung, Y., Threlfall, C., Tonietto, R., Tothmeresz, B., Tscharntke, T., Turner, E., Tylianakis, J., Vanbergen, A., Vassilev, K., Verboven, H., Vergara, C., Vergara, P., Verhulst, J., Walker, T., Wang, Y., Watling, J., Wells, K., Williams, C., Willig, M., Woinarski, J., Wolf, J., Woodcock, B., Yu, D., Zaitsev, A., Collen, B., Ewers, R., Mace, G., Purves, D., Scharlemann, J., and Purvis, A.

Abstract

Biodiversity continues to decline in the face of increasing anthropogenic pressures such as habitat destruction, exploitation, pollution and introduction of alien species. Existing global databases of species’ threat status or population time series are dominated by charismatic species. The collation of datasets with broad taxonomic and biogeographic extents, and that support computation ofa range of biodiversity indicators, is necessary to enable better understanding of historical declines and to project – and avert – future declines. We describe and assess a new database of more than 1.6 million samples from 78 countries representing over 28,000 species, collated from existing spatial comparisons of local-scale biodiversity exposed to different intensities and types of anthropogenic pressures, from terrestrial sites around the world. The database containsmeasurements taken in 208 (of 814) ecoregions, 13 (of 14) biomes, 25 (of 35) biodiversity hotspots and 16 (of 17) megadiverse countries. The database contains more than 1% of the total number of all species described, and more than 1% of the described species within many taxonomic groups – including flowering plants, gymnosperms, birds, mammals, reptiles, amphibians, beetles, lepidopterans and hymenopterans. The dataset, which is still being added to, is therefore already considerably larger and more representative than those used by previous quantitative models of biodiversity trends and responses. The database is being assembled as part of the PREDICTS project (Projecting Responses of Ecological Diversity In Changing Terrestrial Systems – www.predicts.org.uk).

Published
2014

34. Effects of natural resource development on the terrestrial biodiversity of Canadian boreal forests1.

Author

Venier, L.A., Thompson, I.D., Fleming, R., Malcolm, J., Aubin, I., Trofymow, J.A., Langor, D., Sturrock, R., Patry, C., Outerbridge, R.O., Holmes, S.B., Haeussler, S., De Grandpré, L., Chen, H.Y.H., Bayne, E., Arsenault, A., and Brandt, J.P.

Subjects
FOREST management, TAIGAS, PLANT growth, HABITATS, NATURAL resources
Abstract

Copyright of Environmental Reviews is the property of Canadian Science Publishing and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Published
2014
Full Text
View/download PDF

36. Aggrecan degradation in human cartilage. Evidence for both matrix metalloproteinase and aggrecanase activity in normal, osteoarthritic, and rheumatoid joints.

Author

Lark, M W, primary, Bayne, E K, additional, Flanagan, J, additional, Harper, C F, additional, Hoerrner, L A, additional, Hutchinson, N I, additional, Singer, I I, additional, Donatelli, S A, additional, Weidner, J R, additional, Williams, H R, additional, Mumford, R A, additional, and Lohmander, L S, additional

Published
1997
Full Text
View/download PDF

41. Predation on artificial nests in relation to forest type: contrasting the use of quail and plasticine eggs.

Author

Bayne, E. M., Hobson, K. A., and Fargey, P.

Subjects
*BIRDS
Abstract

Previous studies of avian nest predation have focused on how human-induced changes in the landscape influence the frequency of predation.However, natural variation in the abundance of predators due to their choice of habitat can also influence predation rate. To determine if predation on artificial nests was influenced by forest stand type, we placed ground and shrub nests containing quail and plasticine eggsin contiguous coniferous, mixedwood and deciduous stands in the southern boreal mixedwood forest of central Canada. Nest predators were identified using remotely triggered cameras and marks left in plasticine eggs, while the relative abundance of nest predators such as squirrels and corvids were estimated using acoustic-visual surveys. Using the fate of quail eggs to calculate predation rate, we found that predation was significantly higher in coniferous (67%) than in deciduous(17%) or mixedwood (25%) forest, with similar predation on ground (37%) and shrub (29%) nests. Using plasticine eggs to calculate predation rate, nests in coniferous forest still suffered higher rates of predation, although predation rates were 15--20% higher, and ground nests suffered significantly higher rates of predation than shrub nests.Quail eggs seemed to suffer lower rates of predation because small mammals were unable to penetrate the shell, but could leave marks on plasticine eggs. The higher predation rate in coniferous forest was likely caused by higher abundance of red squirrels Tamiasciurus hudsonicus, the presence of fishers Martes pennanti and a simplified understory which may have made it easier for predators to find nests relative to the deciduous and niixedwood forest. Plasticine eggs provide newinsights into nest predation by identifying predation events by smaller predators such as mice that are missed when using quail eggs. [ABSTRACT FROM AUTHOR]

Published
1997
Full Text
View/download PDF

42. Lifestyle-only intervention attenuates the inflammatory state associated with obesity: A randomized controlled study in adolescents

Author

Balagopal, P., George, D., Patton, N., Yarandi, H., Roberts, W.L., Bayne, E., and Gidding, S.

Abstract

ObjectivesThe primary goals were to understand the relationship among the inflammatory factors, C-reactive protein (CRP), interleukin-6 (IL-6), and fibrinogen, and indices of obesity in normoglycemic, insulin-resistant adolescents and to investigate the impact of a lifestyle-only intervention on these nontraditional risk factors for cardiovascular disease (CVD).Study designRandomized controlled lifestyle-only intervention study in adolescents. Of the 21 adolescents studied, 15 obese subjects (body mass index [BMI]=37.6 +/- 3.3 kg/m^2) were randomized to either a lifestyle intervention program or usual care. The lean controls were studied only at baseline. Analysis of variance (ANOVA) for repeated measures was used to study intervention effect and t test, one-way ANOVA, and discriminant function analysis for baseline comparisons.ResultsThe intervention group maintained weight, whereas the control group gained weight (P=.02). A redistribution of body composition and a decrease in insulin resistance were observed. Elevated circulating concentrations of CRP, fibrinogen, and IL-6 were significantly reduced (all P @?.02) in response to intervention, but not in controls.ConclusionsModest lifestyle-only change in previously sedentary obese adolescents redistributes the parameters of body composition in the absence of weight loss and reverses, at least in part, the inflammatory state in association with an improvement of insulin resistance.

Published
2005
Full Text
View/download PDF

46. Extracellular matrix organization in developing muscle: correlation with acetylcholine receptor aggregates.

Author

Bayne, E K, Anderson, M J, and Fambrough, D M

Abstract

Monoclonal antibodies recognizing laminin, heparan sulfate proteoglycan, fibronectin, and two apparently novel connective tissue components have been used to examine the organization of extracellular matrix of skeletal muscle in vivo and in vitro. Four of the five monoclonal antibodies are described for the first time here. Immunocytochemical experiments with frozen-sectioned muscle demonstrated that both the heparan sulfate proteoglycan and laminin exhibited staining patterns identical to that expected for components of the basal lamina. In contrast, the remaining matrix constituents were detected in all regions of muscle connective tissue: the endomysium, perimysium, and epimysium. Embryonic muscle cells developing in culture elaborated an extracellular matrix, each antigen exhibiting a unique distribution. Of particular interest was the organization of extracellular matrix on myotubes: the build-up of matrix components was most apparent in plaques overlying clusters of an integral membrane protein, the acetylcholine receptor (AChR). The heparan sulfate proteoglycan was concentrated at virtually all AChR clusters and showed a remarkable level of congruence with receptor organization; laminin was detected at 70-95% of AChR clusters but often was not completely co-distributed with AChR within the cluster; fibronectin and the two other extracellular matrix antigens occurred at approximately 20, 8, and 2% of the AChR clusters, respectively, and showed little or no congruence with AChR. From observations on the distribution of extracellular matrix components in tissue cultured fibroblasts and myogenic cells, several ideas about the organization of extracellular matrix are suggested. (a) Congruence between AChR clusters and heparan sulfate proteoglycan suggests the existence of some linkage between the two molecules, possibly important for regulation of AChR distribution within the muscle membrane. (b) The qualitatively different patterns of extracellular matrix organization over myotubes and fibroblasts suggest that each of these cell types uses somewhat different means to regulate the assembly of extracellular matrix components within its domain. (c) The limited co-distribution of different components within the extracellular matrix in vitro and the selective immune precipitation of each antigen from conditioned medium suggest that each extracellular matrix component is secreted in a form that is not complexed with other matrix constituents.

Published
1984
Full Text
View/download PDF

47. Multiple forms of (Na+ + K+)-ATPase in the chicken. Selective detection of the major nerve, skeletal muscle, and kidney form by a monoclonal antibody.

Author

Fambrough, D M and Bayne, E K

Abstract

Evidence is presented that monoclonal gamma-immunoglobulin secreted by hybridoma-24 recognizes the sodium and potassium ion-stimulated ATPase of neurons, muscle fibers, and kidney tubule cells in the chicken. The antigen consists of alpha (Mr = 105,000) and beta (Mr = 47,000) subunits. Only the beta subunit bears major oligosaccharide additions (including binding sites for wheat germ agglutinin) to its polypeptide core (Mr = 32,000). The detergent-solubilized antigen sediments as a 7 S complex of alpha beta or alpha beta 2. The antigen has a basolateral distribution in the plasma membrane of renal tubule cells, and in myogenic cell cultures there is up-regulation of the antigen in low potassium medium. Monoclonal antibody-24 specifically recognizes purified gull salt gland (Na+ + K+)-ATPase as well as the major candidate molecule for the (Na+ + K+)-ATPase in enriched preparations from chicken kidney. On cultured myotubes, the number of ouabain-binding sites (4.8 X 10(5)/nucleus) and antigenic sites (4.4 X 10(5)) are approximately equal. However, the antigenic sites on fibroblasts (1.1 X 10(4)) account for only about 4% of the ouabain-binding sites, and there is little or no antibody binding to capillary endothelial cells, Schwann cells, or erythrocytes. Evidence is presented that the antigenic determinant is proteinaceous. It is concluded that at least two antigenically distinct (Na+ + K+)-ATPases exist in the chicken.

Published
1983
Full Text
View/download PDF

48. Immunocytochemical detection of interleukin 1 within stimulated human monocytes.

Author

Bayne, E K, Rupp, E A, Limjuco, G, Chin, J, and Schmidt, J A

Abstract

We have used synthetic peptides coupled to KLH to raise high titer antisera to human IL-1 beta, and in the present report show the usefulness of these sera for immunocytochemical analyses of IL-1 production. Using indirect immunofluorescence, we have been able to specifically identify IL-1 within human monocytes and to monitor its accumulation with time. After indirect immunofluorescent staining of LPS- and PHA-stimulated mononuclear cell cultures, intense cytoplasmic fluorescence was observed in 93% of the monocytes, but not in lymphocytes or platelets present in the same preparation. Unstimulated monocytes did not contain immunocytochemically detectable IL-1. When put into culture, however, some of the otherwise unstimulated monocytes subsequently showed a transient accumulation of intracellular IL-1. Monocytes cultured in the presence of LPS and PHA exhibited detectable fluorescence after 2.5 h, and the fluorescent intensity of these cells continued to increase over the course of 21 h. Fluorescent staining was abolished by preincubation of the sera with relevant but not irrelevant peptide, and while preimmune or anti-KLH serum produced no staining, antisera against either the amino terminus or an internal region of IL-1 beta produced identical staining patterns. Immunoblot analyses of lysates from stimulated monocytes showed that the antisera against IL-1 recognize a single intracellular species with an apparent molecular weight (33 kD) similar to that predicted for IL-1 precursor from the nucleotide sequence of IL-1 cDNA. The ability to specifically identify and immunocytochemically localize IL-1 within producing cells should prove extremely useful for studying the in situ production of IL-1 in immune-based and inflammatory diseases.

Published
1986
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results