19 results on '"Bayasula, Bayasula"'
Search Results
2. Tamoxifen Activates Dormant Primordial Follicles in Mouse Ovaries
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Wei, Wei, Komatsu, Kouji, Osuka, Satoko, Murase, Tomohiko, Bayasula, Bayasula, Nakanishi, Natsuki, Nakamura, Tomoko, Goto, Maki, Iwase, Akira, Masubuchi, Satoru, and Kajiyama, Hiroaki
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- 2022
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3. Upregulated Ribosomal Pathway Impairs Follicle Development in a Polycystic Ovary Syndrome Mouse Model: Differential Gene Expression Analysis of Oocytes
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Nakanishi, Natsuki, primary, Osuka, Satoko, additional, Kono, Tomohiro, additional, Kobayashi, Hisato, additional, Ikeda, Shinya, additional, Bayasula, Bayasula, additional, Sonehara, Reina, additional, Murakami, Mayuko, additional, Yoshita, Sayako, additional, Miyake, Natsuki, additional, Muraoka, Ayako, additional, Kasahara, Yukiyo, additional, Murase, Tomohiko, additional, Nakamura, Tomoko, additional, Goto, Maki, additional, Iwase, Akira, additional, and Kajiyama, Hiroaki, additional
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- 2022
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4. Upregulated Ribosomal Pathway Impairs Follicle Development in a Polycystic Ovary Syndrome Mouse Model: Differential Gene Expression Analysis of Oocytes
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Natsuki Nakanishi, Satoko Osuka, Tomohiro Kono, Hisato Kobayashi, Shinya Ikeda, Bayasula Bayasula, Reina Sonehara, Mayuko Murakami, Sayako Yoshita, Natsuki Miyake, Ayako Muraoka, Yukiyo Kasahara, Tomohiko Murase, Tomoko Nakamura, Maki Goto, Akira Iwase, and Hiroaki Kajiyama
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Obstetrics and Gynecology - Abstract
Polycystic ovary syndrome (PCOS), a common endocrine disorder, is associated with impaired oocyte development, leading to infertility. However, the pathogenesis of PCOS has not been completely elucidated. This study aimed to determine the differentially expressed genes (DEGs) and epigenetic changes in the oocytes from a PCOS mouse model to identify the etiological factors. RNA-sequencing analysis revealed that 90 DEGs were upregulated and 27 DEGs were downregulated in mice with PCOS compared with control mice. DNA methylation analysis revealed 30 hypomethylated and 10 hypermethylated regions in the PCOS group. However, the DNA methylation status did not correlate with differential gene expression. The pathway enrichment analysis revealed that five DEGs (Rps21, Rpl36, Rpl36a, Rpl37a, and Rpl22l1) were enriched in ribosome-related pathways in the oocytes of mice with PCOS, and the immunohistochemical analysis revealed significantly upregulated expression levels of Rps21 and Rpl36. These results suggest that differential gene expression in the oocytes of mice in PCOS is related to impaired folliculogenesis. These findings improve our understanding of PCOS pathogenesis.
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- 2022
5. Upregulated Ribosomal Pathway Impairs Follicle Development in a Polycystic Ovary Syndrome Mouse Model: Findings of Differential Gene Expression Analysis of Oocytes
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Natsuki Nakanishi, Satoko Osuka, Tomohiro Kono, Hisato Kobayashi, Shinya Ikeda, Bayasula Bayasula, Tomomi Seki, Atsushi Yabuki, Hideaki Tanaka, Reina Sonehara, Mayuko Murakami, Sayako Yoshita, Natsuki Miyake, Ayako Muraoka, Yukiyo Kasahara, Tomohiko Murase, Tomoko Nakamura, Maki Goto, Akira Iwase, and Hiroaki Kajiyama
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endocrine system diseases - Abstract
Background: Polycystic ovary syndrome (PCOS), a common endocrinal disorder, is associated with impaired oocyte development, which leads to infertility. However, the pathogenesis of PCOS has not been completely elucidated. Limited studies have analyzed the pathological characteristics of oocytes in PCOS. This study aimed to analyze the differentially expressed genes (DEGs) and epigenetic changes in the oocytes of the PCOS mouse model to identify the etiological factors.Methods: C57BL/6J female mice were subcutaneously injected with vehicle or 5α-dihydrotestosterone (250 µg/day) on days 16–18 of pregnancy. Female offspring were used as the control or PCOS group. The oocytes were collected from mice aged 7–9 weeks. The DEGs between the control and PCOS groups were analyzed using RNA sequencing (RNA-Seq). Additionally, the DNA methylation status was analyzed using the post-bisulfite adaptor tagging method. The ovarian tissue sections were stained with hematoxylin and eosin to examine the morphological changes. The proteins, Rps21 and Rpl36, were measured using immunostaining.Results: Compared with the control group, the PCOS group exhibited impaired estrous cycle and polycystic ovary-like morphology. RNA-Seq analysis revealed that 90 DEGs were upregulated and 27 DEGs were downregulated in the PCOS mouse model. DNA methylation analysis revealed 30 hypomethylated and 10 hypermethylated regions in the PCOS group. However, the DNA methylation status was not correlated with differential gene expression. The Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis revealed that five DEGs (Rps21, Rpl36, Rpl36a, Rpl37a, and Rpl22l1) were enriched in ribosome-related pathways. The immunohistochemical analysis revealed that the expression levels of Rps21 and Rpl36 were significantly upregulated in the PCOS mouse model.Conclusions: These results suggest that differential gene expression in the oocytes of the PCOS mouse model is related to impaired folliculogenesis. These findings improved our understanding of the pathogenesis of PCOS.
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- 2021
6. Differential Gene Expression Analysis of Oocytes in a PCOS Mouse Model Revealed the Relation of Ribosomal Pathway
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Nakanishi, Natsuki, primary, Osuka, Satoko, additional, Kono, Tomohiro, additional, Kobayashi, Hisato, additional, Ikeda, Shinya, additional, Bayasula, Bayasula, additional, Seki, Tomomi, additional, Yabuki, Atsushi, additional, Tanaka, Hideaki, additional, Sonehara, Reina, additional, Murakami, Mayuko, additional, Yoshita, Sayako, additional, Miyake, Natsuki, additional, Muraoka, Ayako, additional, Kasahara, Yukiyo, additional, Murase, Tomohiko, additional, Nakamura, Tomoko, additional, Goto, Maki, additional, Iwase, Akira, additional, and Kajiyama, Hiroaki, additional
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- 2022
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7. Upregulated Ribosomal Pathway Impairs Follicle Development in a Polycystic Ovary Syndrome Mouse Model: Findings of Differential Gene Expression Analysis of Oocytes
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Nakanishi, Natsuki, primary, Osuka, Satoko, additional, Kono, Tomohiro, additional, Kobayashi, Hisato, additional, Ikeda, Shinya, additional, Bayasula, Bayasula, additional, Seki, Tomomi, additional, Yabuki, Atsushi, additional, Tanaka, Hideaki, additional, Sonehara, Reina, additional, Murakami, Mayuko, additional, Yoshita, Sayako, additional, Miyake, Natsuki, additional, Muraoka, Ayako, additional, Kasahara, Yukiyo, additional, Murase, Tomohiko, additional, Nakamura, Tomoko, additional, Goto, Maki, additional, Iwase, Akira, additional, and Kajiyama, Hiroaki, additional
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- 2021
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8. Effectiveness of NLRP3 Inhibitor as a Non-Hormonal Treatment for ovarian endometriosis
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Mayuko Murakami, Satoko Osuka, Ayako Muraoka, Shotaro Hayashi, Bayasula Bayasula, Yukiyo Kasahara, Reina Sonehara, Yumi Hariyama, Kanako Shinjo, Hideaki Tanaka, Natsuki Miyake, Sayako Yoshita, Natsuki Nakanishi, Tomoko Nakamura, Maki Goto, and Kajiyama Hiroaki
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Sulfonamides ,Inflammasomes ,Endometriosis ,Obstetrics and Gynecology ,Mice ,Endocrinology ,Reproductive Medicine ,Indenes ,NLR Family, Pyrin Domain-Containing 3 Protein ,Animals ,Humans ,Female ,Furans ,Developmental Biology - Abstract
Background Endometriosis is a complex syndrome characterized by an estrogen-dependent chronic inflammatory process that affects 10% of women of reproductive age. Ovarian endometriosis (OE) is the most common lesion in endometriosis and may cause infertility, in addition to dysmenorrhea. Hormonal treatments, which are the conventional treatment methods for endometriosis, suppress ovulation and hence are not compatible with fertility. The inflammasome is a complex that includes Nod-like receptor (NLR) family proteins, which sense pathogen-associated molecular patterns and homeostasis-altering molecular processes. It has been reported that the nucleotide-binding oligomerization domain, leucine-rich repeat, and pyrin domain-containing (NLRP) 3 inflammasome, which contributes to the activation of interleukin-1 beta (IL-1β), might be related to the progression of endometriosis. Therefore, the aim of the present study was to evaluate non-hormonal therapies for OE, such as inhibitors of the NLRP3 inflammasome. Methods The expression of NLRP3 was measured in the eutopic endometrium (EM) of patients with and without endometriosis and OE samples, as well as stromal cells derived from the endometrium of patients with and without endometriosis and OE samples (endometrial stromal cells with endometriosis [ESCs] and cyst-derived stromal cells [CSCs]). The effects of an NLRP3 inhibitor (MCC950) on ESCs and CSCs survival and IL-1β production were evaluated. We then administered MCC950 to a murine model of OE to evaluate its effects on OE lesions and ovarian function. Results NLRP3 gene and protein expression levels were higher in OE and CSCs than in EM and ESCs, respectively. MCC950 treatment significantly reduced the survival of CSCs, but not that of ESCs. Moreover, MCC950 treatment reduced the co-localization of NLRP3 and IL-1β in CSCs, as well as IL-1β concentrations in CSCs supernatants. In the murine model, MCC950 treatment reduced OE lesion size compared to phosphate-buffered saline treatment (89 ± 15 vs. 49 ± 9.3 mm3 per ovary; P Conclusions These results indicated that NLRP3/IL-1β is involved in the pathogenesis of endometriosis and that NLRP3 inhibitors may be useful for suppressing OE and improving the function of ovaries with endometriosis.
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- 2021
9. Effectiveness of NLRP3 Inhibitor as a Non-Hormonal Treatment for Ovarian Endometriosis
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Murakami, Mayuko, primary, Osuka, Satoko, additional, Muraoka, Ayako, additional, Hayashi, Shotaro, additional, Bayasula, Bayasula, additional, Kasahara, Yukiyo, additional, Sonehara, Reina, additional, Hariyama, Yumi, additional, Shinjo, Kanako, additional, Tanaka, Hideaki, additional, Miyake, Natsuki, additional, Yoshita, Sayako, additional, Nakanishi, Natsuki, additional, Nakamura, Tomoko, additional, Goto, Maki, additional, and Hiroaki, Kajiyama, additional
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- 2021
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10. Effect of the neuropeptide phoenixin and its receptor GPR173 during folliculogenesis
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Natsuki Nakanishi, Tomohiko Murase, Yukiyo Kasahara, Xuan Phuoc Nguyen, Shotaro Hayashi, Ayako Muraoka, Satoko Osuka, Tomoko Nakamura, Maki Goto, Akira Iwase, Bayasula Bayasula, Fumitaka Kikkawa, and Takashi Nagai
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Adult ,endocrine system ,Embryology ,Granulosa cell ,Population ,Biology ,Receptors, G-Protein-Coupled ,Mice ,Young Adult ,Paracrine signalling ,Endocrinology ,Ovarian Follicle ,Follicular phase ,medicine ,Animals ,Humans ,Ovarian follicle ,Receptor ,Autocrine signalling ,education ,Cell Proliferation ,education.field_of_study ,Granulosa Cells ,Neuropeptides ,Gene Expression Regulation, Developmental ,Obstetrics and Gynecology ,Cell Biology ,Cell biology ,medicine.anatomical_structure ,Reproductive Medicine ,Female ,Folliculogenesis - Abstract
Folliculogenesis is a complex process, defined by the growth and development of follicles from the primordial population. Granulosa cells (GCs) play a vital role in every stage of follicular growth through proliferation, acquisition of gonadotropic responsiveness, steroidogenesis and production of autocrine/paracrine factors. A recently discovered hypothalamic neuropeptide phoenixin is involved in the regulation of the reproductive system. Phoenixin acts through its receptor, G protein-coupled receptor 173 (GPR173), to activate the cAMP/PKA pathway leading to the phosphorylation of CREB (pCREB). Here, we demonstrated the expression patterns of phoenixin and GPR173 in human ovary and explored its role in folliculogenesis. Phoenixin and GPR173 were both expressed in the human ovarian follicle, with increased expression in GCs as the follicle grows. Phoenixin treatment at 100 nM for 24 h induced the proliferation of human non-luteinized granulosa cell line, HGrC1 and significantly increased the expression levels of CYP19A1, FSHR, LHR and KITL, but decreased NPPC expression levels. These effects were suppressed by GPR173 siRNA. The expression level of CREB1, pCREB and estradiol (E2) production in the culture medium was significantly enhanced by phoenixin treatment in a concentration-dependent manner. Phoenixin also significantly increased the follicular area in a murine ovarian tissue culture model, leading to an increased number of ovulated oocytes with a higher level of maturation. Taken together, our data demonstrate that phoenixin is an intraovarian factor that promotes follicular growth through its receptor GPR173 by accelerating proliferation of GCs, inducing E2 production and increasing the expression of genes related to follicle development.
- Published
- 2019
11. Usefulness of ultrasensitive anti-Müllerian hormone assay to predict follicle growth in patients with primary ovarian insufficiency
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Bayasula Bayasula, Fumitaka Kikkawa, Akira Iwase, Yukiyo Kasahara, Satoko Osuka, Tomoko Nakamura, Natsuki Nakanishi, Tomohiko Murase, Maki Goto, and Tomomi Kotani
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endocrine system ,medicine.medical_specialty ,endocrine system diseases ,biology ,business.industry ,Primary ovarian insufficiency ,Anti-Müllerian hormone ,Endocrinology ,Internal medicine ,biology.protein ,Medicine ,In patient ,business ,hormones, hormone substitutes, and hormone antagonists ,Follicle growth - Abstract
Background: Patients with primary ovarian insufficiency (POI) present with follicle growth occasionally, however, it is difficult to predict the exact cycles with follicle growth. Serum anti-Müllerian hormone (AMH), a useful marker for ovarian reserve, is produced in early-stage follicles. Therefore, AMH levels should reflect the existence of small follicles which are difficult to detect using ultrasonography and may be useful for predicting follicle growth in patients with POI. Methods: Using an ultrasensitive enzyme-linked immunosorbent assay (ELISA) kit, we found very low serum AMH levels of patients with POI; we further assessed the follicle growth of each patient and their cycles to clarify the usefulness of measuring serum AMH levels for predicting the follicle growth in patients with POI. Results: A total of 91 cycles of 19 patients with POI were analyzed in this study. Five patients presented with positive serum AMH levels during the observational periods and all five experienced follicle growth. Only two of the 14 patients with negative serum AMH levels had follicle growth. Serum AMH and follicle-stimulating hormone (FSH) levels in cycles with follicle growth were significantly higher than in cycles without follicle growth. The median serum AMH level (2.77 pg/mL; 25th, 75th percentiles: 0.0, 9.64) in cycles with follicle growth were lower than the lower limit of detection of conventional AMH ELISA kits. The positive predictive value of positive serum AMH levels for follicle growth was higher than that of FSH (
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- 2020
12. The post-operative decline in serum anti-Müllerian hormone correlates with the bilaterality and severity of endometriosis
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Hirokawa, Wakana, Iwase, Akira, Goto, Maki, Takikawa, Sachiko, Nagatomo, Yoshinari, Nakahara, Tatsuo, Bayasula, Bayasula, Nakamura, Tomoko, Manabe, Shuichi, and Kikkawa, Fumitaka
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- 2011
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13. Very low levels of serum anti-Müllerian hormone as a possible marker for follicle growth in patients with primary ovarian insufficiency under hormone replacement therapy
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Kasahara, Yukiyo, primary, Osuka, Satoko, additional, Bayasula, Bayasula, additional, Nakanishi, Natsuki, additional, Murase, Tomohiko, additional, Nakamura, Tomoko, additional, Goto, Maki, additional, Kotani, Tomomi, additional, Iwase, Akira, additional, and Kikkawa, Fumitaka, additional
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- 2020
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14. Usefulness of ultrasensitive anti-Müllerian hormone assay to predict follicle growth in patients with primary ovarian insufficiency
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Kasahara, Yukiyo, primary, Osuka, Satoko, additional, Bayasula, Bayasula, additional, Nakanishi, Natsuki, additional, Murase, Tomohiko, additional, Nakamura, Tomoko, additional, Goto, Maki, additional, Kotani, Tomomi, additional, Iwase, Akira, additional, and Kikkawa, Fumitaka, additional
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- 2020
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15. Effect of the neuropeptide phoenixin and its receptor GPR173 during folliculogenesis
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Nguyen, Xuan Phuoc, primary, Nakamura, Tomoko, additional, Osuka, Satoko, additional, Bayasula, Bayasula, additional, Nakanishi, Natsuki, additional, Kasahara, Yukiyo, additional, Muraoka, Ayako, additional, Hayashi, Shotaro, additional, Nagai, Takashi, additional, Murase, Tomohiko, additional, Goto, Maki, additional, Iwase, Akira, additional, and Kikkawa, Fumitaka, additional
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- 2019
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16. CYP51A1 Induced by Growth Differentiation Factor 9 and Follicle-Stimulating Hormone in Granulosa Cells Is a Possible Predictor for Unfertilization
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Yoshinari Nagatomo, Sachiko Takikawa, Tomoko Nakamura, Mika Kondo, Akira Iwase, Fumitaka Kikkawa, Tohru Kiyono, Tomomi Kotani, Tatsuo Nakahara, Bayasula Bayasula, and Maki Goto
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Adult ,endocrine system ,medicine.medical_specialty ,Transcription, Genetic ,Granulosa cell ,Growth Differentiation Factor 9 ,Fertilization in Vitro ,Growth differentiation factor-9 ,Biology ,Cell Line ,Sterol 14-Demethylase ,Follicle-stimulating hormone ,Internal medicine ,Follicular phase ,medicine ,Humans ,RNA, Messenger ,Treatment Failure ,Receptor ,Messenger RNA ,Granulosa Cells ,Obstetrics and Gynecology ,Original Articles ,Reverse transcription polymerase chain reaction ,Cholesterol ,Endocrinology ,Cell culture ,Enzyme Induction ,Female ,Follicle Stimulating Hormone ,Biomarkers - Abstract
Growth differentiation factor 9 (GDF9), an oocyte-secreted factor, whose receptors exist in granulosa cells, is involved in follicle progression. Therefore, GDF9 is considered to potentially mediate signals necessary for follicular growth. However, the effect of GDF9 on human granulosa cells is not fully understood. Human immortalized nonluteinized granulosa cell line (HGrC1) which we have previously reported was stimulated with GDF9 and/or follicle-stimulating hormone (FSH). Granulosa cells obtained from in vitro fertilization (IVF) patients were also evaluated with quantitative reverse transcription polymerase chain reaction (RT-PCR). Real-time RT-PCR showed that GDF9 increased messenger RNA (mRNA) levels of enzymes required for cholesterol biosynthesis, such as 3-hydroxy-3-methylglutanyl-CoA synthase 1 (HMGCS1), farnesyl-diphosphate farnesyltransferase 1, squalene epoxidase, lanosterol synthase, and cytochrome P450, family 51, subfamily A, polypeptide 1 (CYP51A1). A greater increase in mRNA levels of HMGCS1 and CYP51A1 was observed by combined treatment with GDF9 and FSH. Clinical samples showed a significant increase in CYP51A1 mRNA in the group of granulosa cells connected with unfertilized oocytes. Our results suggest that GDF9, possibly with FSH, may play significant roles in the regulation of cholesterol biosynthesis and the expression of CYP51A1 which might be a predictor for unfertilization.
- Published
- 2015
17. Session 68: Oocyte Maturation
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Barbro E. Fridén, Linda Wordeman, J. Xu, N. Staubach, Wakana Hirokawa, R. Firestone, Julius Hreinsson, O. Fainaru, K. Gulle, Hugh J. Clarke, Isabelle Demeestere, Sachiko Takikawa, Fumitaka Kikkawa, Peter Sjöblom, Bayasula Bayasula, Hiroshi Kobayashi, Maki Goto, I. Cino, E. Mahrous, M. Sanhaij, E. Gismano, S. El-Khabouri, Denny Sakkas, Maria D. Lalioti, M.M. Rahman, E. Vogt, Tiziana A. L. Brevini, E. Shalom-Paz, Tatsuo Nakahara, M Fridström, Akira Iwase, Georgia Pennarossa, Shuichi Manabe, L. De Santis, Thorsten Seidel, Ursula Eichenlaub-Ritter, W. Klein, Robert F. Casper, A.K.M. Streiff, F. Calzi, Elisa Rabellotti, Yoshinari Nagatomo, and H. Qian
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Andrology ,medicine.anatomical_structure ,Reproductive Medicine ,business.industry ,Rehabilitation ,Obstetrics and Gynecology ,Medicine ,Session (computer science) ,business ,Oocyte - Published
- 2010
18. Effect of the neuropeptide phoenixin and its receptor GPR173 during folliculogenesis.
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Xuan Phuoc Nguyen, Tomoko Nakamura, Satoko Osuka, Bayasula Bayasula, Natsuki Nakanishi, Yukiyo Kasahara, Ayako Muraoka, Shotaro Hayashi, Takashi Nagai, Tomohiko Murase, Maki Goto, Akira Iwase, and Fumitaka Kikkawa
- Subjects
NEUROPEPTIDES ,G protein coupled receptors ,OVARIAN follicle ,GRANULOSA cells ,GENITALIA ,TISSUE culture - Abstract
Folliculogenesis is a complex process, defined by the growth and development of follicles from the primordial population. Granulosa cells (GCs) play a vital role in every stage of follicular growth through proliferation, acquisition of gonadotropic responsiveness, steroidogenesis and production of autocrine/paracrine factors. A recently discovered hypothalamic neuropeptide phoenixin is involved in the regulation of the reproductive system. Phoenixin acts through its receptor, G protein-coupled receptor 173 (GPR173), to activate the cAMP/PKA pathway leading to the phosphorylation of CREB (pCREB). Here, we demonstrated the expression patterns of phoenixin and GPR173 in human ovary and explored its role in folliculogenesis. Phoenixin and GPR173 were both expressed in the human ovarian follicle, with increased expression in GCs as the follicle grows. Phoenixin treatment at 100 nM for 24 h induced the proliferation of human non-luteinized granulosa cell line, HGrC1 and significantly increased the expression levels of CYP19A1, FSHR, LHR and KITL, but decreased NPPC expression levels. These effects were suppressed by GPR173 siRNA. The expression level of CREB1, pCREB and estradiol (E2) production in the culture medium was significantly enhanced by phoenixin treatment in a concentration-dependent manner. Phoenixin also significantly increased the follicular area in a murine ovarian tissue culture model, leading to an increased number of ovulated oocytes with a higher level of maturation. Taken together, our data demonstrate that phoenixin is an intraovarian factor that promotes follicular growth through its receptor GPR173 by accelerating proliferation of GCs, inducing E2 production and increasing the expression of genes related to follicle development. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
19. The post-operative decline in serum anti-Mullerian hormone correlates with the bilaterality and severity of endometriosis
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Wakana Hirokawa, Maki Goto, Bayasula Bayasula, Fumitaka Kikkawa, Yoshinari Nagatomo, Tomoko Nakamura, Sachiko Takikawa, Akira Iwase, Shuichi Manabe, and Tatsuo Nakahara
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Adult ,Anti-Mullerian Hormone ,endocrine system ,medicine.medical_specialty ,endocrine system diseases ,Adolescent ,medicine.medical_treatment ,Endometriosis ,Urology ,Ovary ,Preoperative care ,Cystectomy ,Ovarian Follicle ,medicine ,Humans ,Cyst ,Postoperative Period ,Ovarian follicle ,Ovarian reserve ,Gynecology ,biology ,Rehabilitation ,Obstetrics and Gynecology ,Anti-Müllerian hormone ,Middle Aged ,medicine.disease ,female genital diseases and pregnancy complications ,medicine.anatomical_structure ,Treatment Outcome ,Reproductive Medicine ,biology.protein ,Female ,Laparoscopy - Abstract
BACKGROUND To assess the impact of ovarian cystectomy for endometriomas on the ovarian reserve, we evaluated the pre- and post-operative levels of serum anti-Mullerian hormone (AMH). We also analyzed the correlations between factors related to endometriosis and surgery for endometriomas and the serum AMH levels to investigate which factors affect ovarian reserve. METHODS Thirty-eight patients who were undergoing ovarian cystectomy for unilateral endometrioma (n = 20) and bilateral endometriomas (n = 18) participated. Preoperative and post-operative serum samples were collected and assayed for AMH levels, and changes between the two samples were analyzed in association with parameters of endometriosis and surgery for endometriomas. RESULTS The mean AMH level was 3.9 ng/ml prior to surgery, and was reduced to 2.1 ng/ml at 1 month post-surgery. The rate of decline of the serum AMH level was significantly higher in the bilateral group than the unilateral group (62.8 ± 29.6 versus 24.7 ± 32.5%, P < 0.001). The rate of decline in the serum AMH levels showed a significant correlation to the revised American Society for Reproductive Medicine (rASRM) score (P = 0.003), but not age, cyst diameter, blood loss during the operation or the number of follicles removed in the specimens. CONCLUSIONS Our results suggest that the decrease in ovarian reserve should be taken into account in patients indicated for cystectomy for bilateral endometriomas or unilateral endometrioma with high rASRM scores.
- Published
- 2011
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