42 results on '"Bayascas JR"'
Search Results
2. Capturing conformational transitions of full-length PDK1 that dictate kinase substrate selectivity.
- Author
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Martínez-Arenas L and Bayascas JR
- Subjects
- Protein Serine-Threonine Kinases genetics, Signal Transduction
- Abstract
PDK1 is a constitutively active master kinase that can phosphorylate and activate as many as 24 enzymes, all belonging to the AGC family of serine-threonine protein kinases. In this issue of Science Signaling , Sacerdoti et al . uncover how allosteric communication between different functional domains directs the selectivity of PDK1 toward particular subsets of substrates.
- Published
- 2023
- Full Text
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3. The ERK5/NF-κB signaling pathway targets endometrial cancer proliferation and survival.
- Author
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Diéguez-Martínez N, Espinosa-Gil S, Yoldi G, Megías-Roda E, Bolinaga-Ayala I, Viñas-Casas M, Gorgisen G, Domingo-Ortí I, Pérez-Montoyo H, Bayascas JR, Colas E, Dolcet X, and Lizcano JM
- Subjects
- Animals, Carboplatin, Cell Proliferation, Cytokines metabolism, Epidermal Growth Factor metabolism, Female, Humans, MAP Kinase Kinase 5 genetics, MAP Kinase Kinase 5 metabolism, MAP Kinase Signaling System, Mice, Mice, Nude, Paclitaxel pharmacology, Paclitaxel therapeutic use, Endometrial Neoplasms genetics, NF-kappa B genetics, NF-kappa B metabolism
- Abstract
Endometrial cancer (EC) is the most common type of gynecologic cancer in women of developed countries. Despite surgery combined with chemo-/radiotherapy regimens, overall survival of patients with high-risk EC tumors is poor, indicating a need for novel therapies. The MEK5-ERK5 pathway is activated in response to growth factors and to different stressors, including oxidative stress and cytokines. Previous evidence supports a role for the MEK5-ERK5 pathway in the pathology of several cancers. We investigated the role of ERK5 in EC. In silico analysis of the PanCancer Atlas dataset showed alterations in components of the MEK5-ERK5 pathway in 48% of EC patients. Here, we show that ERK5 inhibition or silencing decreased EGF-induced EC cell proliferation, and that genetic deletion of MEK5 resulted in EC impaired proliferation and reduced tumor growth capacity in nude mice. Pharmacologic inhibition or ERK5 silencing impaired NF-kB pathway in EC cells and xenografts. Furthermore, we found a positive correlation between ERK5 and p65/RELA protein levels in human EC tumor samples. Mechanistically, genetic or pharmacologic impairment of ERK5 resulted in downregulation of NEMO/IKKγ expression, leading to impaired p65/RELA activity and to apoptosis in EC cells and xenografts, which was rescued by NEMO/IKKγ overexpression. Notably, ERK5 inhibition, MEK5 deletion or NF-kB inhibition sensitized EC cells to standard EC chemotherapy (paclitaxel/carboplatin) toxicity, whereas ERK5 inhibition synergized with paclitaxel to reduce tumor xenograft growth in mice. Together, our results suggest that the ERK5-NEMO-NF-κB pathway mediates EC cell proliferation and survival. We propose the ERK5/NF-κB axis as new target for EC treatment., (© 2022. The Author(s).)
- Published
- 2022
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4. Molecular Insights into the Regulation of 3-Phosphoinositide-Dependent Protein Kinase 1: Modeling the Interaction between the Kinase and the Pleckstrin Homology Domains.
- Author
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Garcia-Viloca M, Bayascas JR, Lluch JM, and González-Lafont À
- Abstract
The 3-phosphoinositide-dependent protein kinase 1 (PDK1) K465E mutant kinase can still activate protein kinase B (PKB) at the membrane in a phosphatidylinositol-3,4,5-trisphosphate (PIP
3 , PtdIns(3,4,5)P3 ) independent manner. To understand this new PDK1 regulatory mechanism, docking and molecular dynamics calculations were performed for the first time to simulate the wild-type kinase domain-pleckstrin homology (PH) domain complex with PH-in and PH-out conformations. These simulations were then compared to the PH-in model of the KD-PH(mutant K465E) PDK1 complex. Additionally, three KD-PH complexes were simulated, including a substrate analogue bound to a hydrophobic pocket (denominated the PIF-pocket) substrate-docking site. We find that only the PH-out conformation, with the PH domain well-oriented to interact with the cellular membrane, is active for wild-type PDK1. In contrast, the active conformation of the PDK1 K465E mutant is PH-in, being ATP-stable at the active site while the PIF-pocket is more accessible to the peptide substrate. We corroborate that both the docking-site binding and the catalytic activity are in fact enhanced in knock-in mouse samples expressing the PDK1 K465E protein, enabling the phosphorylation of PKB in the absence of PIP3 binding., Competing Interests: The authors declare no competing financial interest., (© 2022 The Authors. Published by American Chemical Society.)- Published
- 2022
- Full Text
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5. Acox2 is a regulator of lysine crotonylation that mediates hepatic metabolic homeostasis in mice.
- Author
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Zhang Y, Chen Y, Zhang Z, Tao X, Xu S, Zhang X, Zurashvili T, Lu Z, Bayascas JR, Jin L, Zhao J, and Zhou X
- Subjects
- Animals, Homeostasis, Mice, Lysine metabolism
- Abstract
Acyl-CoA oxidase 2 (Acox2) is an enzyme involved in peroxisomal bile acid synthesis and branched-chain fatty acid degradation. Acox2 knockout (-/-) mice spontaneously developed liver cancer with marked lymphocytic infiltrate. Tandem-affinity purification coupled with mass spectrometry analysis revealed that Acox2 interacted with methylcrotonoyl-CoA carboxylase followed by co-immunoprecipitation confirmation. Here we reported that non-histone lysine crotonylation (Kcr) levels were downregulated in Acox2
-/- mice livers. Interestingly, Kcr signals were concentrated in the nucleus of tumor cells but mostly located in the cytoplasm of adjacent normal liver cells of Acox2-/- mice. Quantitative analysis of the global crotonylome further revealed that 54% (27/50) of downregulated non-histone Kcr sites were located in mitochondrial (11/50) and peroxisomal (17/50) enzymes including Ehhadh, Scp2, Hsd17b4, Crot, Etfa, Cpt1a, Eci1/2, Hadha, Etfdh, and Idh2. Subsequent site-directed mutagenesis and transcriptome analysis revealed that Ehhadh K572 cr might have site-specific regulatory roles by downregulating TOP3B expression that lead to increased DNA damage in vitro. Our findings suggested Acox2 is a regulator of Kcr that might play critical role on hepatic metabolic homeostasis., (© 2022. The Author(s).)- Published
- 2022
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6. Gossypol Treatment Restores Insufficient Apoptotic Function of DFF40/CAD in Human Glioblastoma Cells.
- Author
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Martínez-Escardó L, Alemany M, Sánchez-Osuna M, Sánchez-Chardi A, Roig-Martínez M, Suárez-García S, Ruiz-Molina D, Vidal N, Plans G, Majós C, Ribas J, Baltrons MA, Bayascas JR, Barcia C, Bruna J, and Yuste VJ
- Abstract
Glioblastoma (GBM) is a highly aggressive brain tumor and almost all patients die because of relapses. GBM-derived cells undergo cell death without nuclear fragmentation upon treatment with different apoptotic agents. Nuclear dismantling determines the point-of-no-return in the apoptotic process. DFF40/CAD is the main endonuclease implicated in apoptotic nuclear disassembly. To be properly activated, DFF40/CAD should reside in the cytosol. However, the endonuclease is poorly expressed in the cytosol and remains cumulated in the nucleus of GBM cells. Here, by employing commercial and non-commercial patient-derived GBM cells, we demonstrate that the natural terpenoid aldehyde gossypol prompts DFF40/CAD-dependent nuclear fragmentation. A comparative analysis between gossypol- and staurosporine-treated cells evidenced that levels of neither caspase activation nor DNA damage were correlated with the ability of each compound to induce nuclear fragmentation. Deconvoluted confocal images revealed that DFF40/CAD was almost completely excluded from the nucleus early after the staurosporine challenge. However, gossypol-treated cells maintained DFF40/CAD in the nucleus for longer times, shaping a ribbon-like structure piercing the nuclear fragments and building a network of bridged masses of compacted chromatin. Therefore, GBM cells can fragment their nuclei if treated with the adequate insult, making the cell death process irreversible.
- Published
- 2021
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7. Sex-Dependent Signatures, Time Frames and Longitudinal Fine-Tuning of the Marble Burying Test in Normal and AD-Pathological Aging Mice.
- Author
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Santana-Santana M, Bayascas JR, and Giménez-Llort L
- Abstract
The marble burying (MB) test, a classical test based on the natural tendency of rodents to dig in diverse substrates and to bury small objects, is sensitive to some intrinsic and extrinsic factors. Here, under emerging neuroethological quantitative and qualitative analysis, the MB performance of 12-month-old male and female 3xTg-AD mice for Alzheimer's disease and age-matched counterparts of gold-standard C57BL6 strain with normal aging unveiled sex-dependent signatures. In addition, three temporal analyses, through the (1) time course of the performance, and (2) a repeated test schedule, identified the optimal time frames and schedules to detect sex- and genotype-dependent differences. Besides, a (3) longitudinal design from 12 to 16 months of age monitored the changes in the performance with aging, worsening in AD-mice, and modulation through the repeated test. In summary, the present results allow us to conclude that (1) the marble burying test is responsive to genotype, sex, aging, and its interactions; (2) the male sex was more sensitive to showing the AD-phenotype; (3) longitudinal assessment shows a reduction in females with AD pathology; (4) burying remains stable in repeated testing; (5) the time-course of marbles burying is useful; and (6) burying behavior most likely represents perseverative and/or stereotyped-like behavior rather than anxiety-like behavior in 3xTg-AD mice.
- Published
- 2021
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8. Fine-Tuning the PI3K/Akt Signaling Pathway Intensity by Sex and Genotype-Load: Sex-Dependent Homozygotic Threshold for Somatic Growth but Feminization of Anxious Phenotype in Middle-Aged PDK1 K465E Knock-In and Heterozygous Mice.
- Author
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Santana-Santana M, Bayascas JR, and Giménez-Llort L
- Abstract
According to the Research Domain Criteria (RDoC), phenotypic differences among disorders may be explained by variations in the nature and degree of neural circuitry disruptions and/or dysfunctions modulated by several biological and environmental factors. We recently demonstrated the in vivo behavioral translation of tweaking the PI3K/Akt signaling, an essential pathway for regulating cellular processes and physiology, and its modulation through aging. Here we describe, for the first time, the in vivo behavioral impact of the sex and genetic-load tweaking this pathway. The anxiety-like phenotypes of 61 mature (11-14-month-old) male and female PDK1 K465E knock-in, heterozygous, and WT mice were studied. Forced (open-field) anxiogenic environmental conditions were sensitive to detect sex and genetic-load differences at middle age. Despite similar neophobia and horizontal activity among the six groups, females exhibited faster ethograms than males, with increased thigmotaxis, increased wall and bizarre rearing. Genotype-load unveiled increased anxiety in males, resembling female performances. The performance of mutants in naturalistic conditions (marble test) was normal. Homozygotic-load was needed for reduced somatic growth only in males. Factor interactions indicated the complex interplay in the elicitation of different negative valence system's items and the fine-tuning of PI3K/Akt signaling pathway intensity by genotype-load and sex.
- Published
- 2021
- Full Text
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9. The Impact of the PI3K/Akt Signaling Pathway in Anxiety and Working Memory in Young and Middle-Aged PDK1 K465E Knock-In Mice.
- Author
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Giménez-Llort L, Santana-Santana M, and Bayascas JR
- Abstract
Dysfunction and dysregulation at the genetic, neural, and behavioral levels point at the fine-tuning of broadly spread networks as critical for a wide array of behaviors and mental processes through the life span. This brain-based evidence, from basic to behavioral neuroscience levels, is leading to a new conceptualization of mental health and disease. Thus, the Research Domain Criteria considers phenotypic differences observed among disorders as explained by variations in the nature and degree of neural circuitry disruptions, under the modulation of several developmental, compensatory, environmental, and epigenetic factors. In this context, we aimed to describe for the first time the in vivo behavioral impact of tweaking the PI3K/Akt signaling pathway known to play an essential role in the regulation of cellular processes, leading to diverse physiological responses. We explored the effects in young (YA, 3-4 months of age) and mature (MA, 11-14 months of age) male and female PDK1 K465E knock-in mice in a battery of tests under different anxiogenic conditions. The results evidenced that the double mutation of the PDK1 pleckstrin homology (PH) domain resulted in an enhancement of the negative valence system shown as an increase of responses of fear- and anxiety-like behaviors in anxiogenic situations. Interestingly, this seemed to be specific of YA and found regulated at middle age. In contrast, cognitive deficits, as measured in a spatial working memory task, were found in both YA and MA mutants and independently of the level of their anxious-like profiles. These distinct age- and function-dependent impacts would be in agreement with the distinct cortical and limbic deficits in the Akt signaling in the brain we have recently described in these same animals. The elicitation of age- and neuronal-dependent specific patterns suggests that fine-tuning the intensity of the PKB/Akt signal that enables diverse physiological response has also its in vivo translation into the negative valence system and age is a key regulatory factor., (Copyright © 2020 Giménez-Llort, Santana-Santana and Bayascas.)
- Published
- 2020
- Full Text
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10. Effects of CK2β subunit down-regulation on Akt signalling in HK-2 renal cells.
- Author
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Alcaraz E, Vilardell J, Borgo C, Sarró E, Plana M, Marin O, Pinna LA, Bayascas JR, Meseguer A, Salvi M, Itarte E, and Ruzzene M
- Subjects
- CRISPR-Cas Systems genetics, Cell Line, Chromones pharmacology, Epithelial-Mesenchymal Transition genetics, Gene Expression Regulation, Enzymologic drug effects, Gene Knockout Techniques, Humans, Kidney drug effects, Kidney metabolism, MAP Kinase Signaling System drug effects, Morpholines pharmacology, Naphthyridines pharmacology, Phenazines, Phosphatidylinositol 3-Kinases genetics, Phosphorylation drug effects, Protein Isoforms, Ribosomal Protein S6 Kinases, 90-kDa genetics, Signal Transduction drug effects, Casein Kinase II genetics, Glycogen Synthase Kinase 3 beta genetics, Oncogene Protein v-akt genetics, Snail Family Transcription Factors genetics
- Abstract
The PI3K/Akt pathway is interconnected to protein kinase CK2, which directly phosphorylates Akt1 at S129. We have previously found that, in HK-2 renal cells, downregulation of the CK2 regulatory subunit β (shCK2β cells) reduces S129 Akt phosphorylation. Here, we investigated in more details how the different CK2 isoforms impact on Akt and other signaling pathways. We found that all CK2 isoforms phosphorylate S129 in vitro, independently of CK2β. However, in HK-2 cells the dependence on CK2β was confirmed by rescue experiments (CK2β re-expression in shCK2β HK-2 cells), suggesting the presence of additional components that drive Akt recognition by CK2 in cells. We also found that CK2β downregulation altered the phosphorylation ratio between the two canonical Akt activation sites (pT308 strongly reduced, pS473 slightly increased) in HK-2 cells. Similar results were found in other cell lines where CK2β was stably knocked out by CRISPR-Cas9 technology. The phosphorylation of rpS6 S235/S236, a downstream effector of Akt, was strongly reduced in shCK2β HK-2 cells, while the phosphorylation of two Akt direct targets, PRAS40 T246 and GSK3β S9, was increased. Differently to what observed in response to CK2β down-regulation, the chemical inhibition of CK2 activity by cell treatment with the specific inhibitor CX-4945 reduced both the Akt canonical sites, pT308 and pS473. In CX-4945-treated cells, the changes in rpS6 pS235/S236 and GSK3β pS9 mirrored those induced by CK2β knock-down (reduction and slight increase, respectively); on the contrary, the effect on PRAS40 pT246 phosphorylation was sharply different, being strongly reduced by CK2 inhibition; this suggests that this Akt target might be dependent on Akt pS473 status in HK-2 cells. Since PI3K/Akt and ERK1/2/p90rsk pathways are known to be interconnected and both modulated by CK2, with GSK3β pS9 representing a convergent point, we investigated if ERK1/2/p90rsk signaling was affected by CK2β knock-down and CX-4945 treatment in HK-2 cells. We found that p90rsk was insensitive to any kind of CK2 targeting; therefore, the observation that, similarly, GSK3β pS9 was not reduced by CK2 blockade suggests that GSK3β phosphorylation is mainly under the control of p90rsk in these cells. However, we found that the PI3K inhibitor LY294002 reduced GSK3β pS9, and concomitantly decreased Snail1 levels (a GSK3β target and Epithelial-to-Mesenchymal transition marker). The effects of LY294002 were observed also in CK2β-downregulated cells, suggesting that reducing GSK3β pS9 could be a strategy to control Snail1 levels in any situation where CK2β is defective, as possibly occurring in cancer cells., Competing Interests: The authors have declared that no competing interests exist.
- Published
- 2020
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11. Reducing the Levels of Akt Activation by PDK1 Knock-in Mutation Protects Neuronal Cultures against Synthetic Amyloid-Beta Peptides.
- Author
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Yang S, Pascual-Guiral S, Ponce R, Giménez-Llort L, Baltrons MA, Arancio O, Palacio JR, Clos VM, Yuste VJ, and Bayascas JR
- Abstract
The Akt kinase has been widely assumed for years as a key downstream effector of the PI3K signaling pathway in promoting neuronal survival. This notion was however challenged by the finding that neuronal survival responses were still preserved in mice with reduced Akt activity. Moreover, here we show that the Akt signaling is elevated in the aged brain of two different mice models of Alzheimer Disease. We manipulate the rate of Akt stimulation by employing knock-in mice expressing a mutant form of PDK1 (phosphoinositide-dependent protein kinase 1) with reduced, but not abolished, ability to activate Akt. We found increased membrane localization and activity of the TACE/ADAM17 α-secretase in the brain of the PDK1 mutant mice with concomitant TNFR1 processing, which provided neurons with resistance against TNFα-induced neurotoxicity. Opposite to the Alzheimer Disease transgenic mice, the PDK1 knock-in mice exhibited an age-dependent attenuation of the unfolding protein response, which protected the mutant neurons against endoplasmic reticulum stressors. Moreover, these two mechanisms cooperatively provide the mutant neurons with resistance against amyloid-beta oligomers, and might singularly also contribute to protect these mice against amyloid-beta pathology.
- Published
- 2018
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12. Mutation of the 3-Phosphoinositide-Dependent Protein Kinase 1 (PDK1) Substrate-Docking Site in the Developing Brain Causes Microcephaly with Abnormal Brain Morphogenesis Independently of Akt, Leading to Impaired Cognition and Disruptive Behaviors.
- Author
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Cordón-Barris L, Pascual-Guiral S, Yang S, Giménez-Llort L, Lope-Piedrafita S, Niemeyer C, Claro E, Lizcano JM, and Bayascas JR
- Abstract
The phosphoinositide (PI) 3-kinase/Akt signaling pathway plays essential roles during neuronal development. 3-Phosphoinositide-dependent protein kinase 1 (PDK1) coordinates the PI 3-kinase signals by activating 23 kinases of the AGC family, including Akt. Phosphorylation of a conserved docking site in the substrate is a requisite for PDK1 to recognize, phosphorylate, and activate most of these kinases, with the exception of Akt. We exploited this differential mechanism of regulation by generating neuron-specific conditional knock-in mice expressing a mutant form of PDK1, L155E, in which the substrate-docking site binding motif, termed the PIF pocket, was disrupted. As a consequence, activation of all the PDK1 substrates tested except Akt was abolished. The mice exhibited microcephaly, altered cortical layering, and reduced circuitry, leading to cognitive deficits and exacerbated disruptive behavior combined with diminished motivation. The abnormal patterning of the adult brain arises from the reduced ability of the embryonic neurons to polarize and extend their axons, highlighting the essential roles that the PDK1 signaling beyond Akt plays in mediating the neuronal responses that regulate brain development., (Copyright © 2016, American Society for Microbiology. All Rights Reserved.)
- Published
- 2016
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13. Correction for Zurashvili et al., Interaction of PDK1 with Phosphoinositides Is Essential for Neuronal Differentiation but Dispensable for Neuronal Survival.
- Author
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Zurashvili T, Cordón-Barris L, Ruiz-Babot G, Zhou X, Lizcano JM, Gómez N, Giménez-Llort L, and Bayascas JR
- Published
- 2016
- Full Text
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14. The New Antitumor Drug ABTL0812 Inhibits the Akt/mTORC1 Axis by Upregulating Tribbles-3 Pseudokinase.
- Author
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Erazo T, Lorente M, López-Plana A, Muñoz-Guardiola P, Fernández-Nogueira P, García-Martínez JA, Bragado P, Fuster G, Salazar M, Espadaler J, Hernández-Losa J, Bayascas JR, Cortal M, Vidal L, Gascón P, Gómez-Ferreria M, Alfón J, Velasco G, Domènech C, and Lizcano JM
- Subjects
- Animals, Cell Death drug effects, Cell Line, Cell Line, Tumor, Cell Proliferation drug effects, Female, Humans, Mice, Mice, Nude, Rats, Signal Transduction drug effects, Antineoplastic Agents pharmacology, Cell Cycle Proteins metabolism, Mechanistic Target of Rapamycin Complex 1 metabolism, Proto-Oncogene Proteins c-akt metabolism, Up-Regulation drug effects
- Abstract
Purpose: ABTL0812 is a novel first-in-class, small molecule which showed antiproliferative effect on tumor cells in phenotypic assays. Here we describe the mechanism of action of this antitumor drug, which is currently in clinical development., Experimental Design: We investigated the effect of ABTL0812 on cancer cell death, proliferation, and modulation of intracellular signaling pathways, using human lung (A549) and pancreatic (MiaPaCa-2) cancer cells and tumor xenografts. To identify cellular targets, we performed in silico high-throughput screening comparing ABTL0812 chemical structure against ChEMBL15 database., Results: ABTL0812 inhibited Akt/mTORC1 axis, resulting in impaired cancer cell proliferation and autophagy-mediated cell death. In silico screening led us to identify PPARs, PPARα and PPARγ as the cellular targets of ABTL0812. We showed that ABTL0812 activates both PPAR receptors, resulting in upregulation of Tribbles-3 pseudokinase (TRIB3) gene expression. Upregulated TRIB3 binds cellular Akt, preventing its activation by upstream kinases, resulting in Akt inhibition and suppression of the Akt/mTORC1 axis. Pharmacologic inhibition of PPARα/γ or TRIB3 silencing prevented ABTL0812-induced cell death. ABTL0812 treatment induced Akt inhibition in cancer cells, tumor xenografts, and peripheral blood mononuclear cells from patients enrolled in phase I/Ib first-in-human clinical trial., Conclusions: ABTL0812 has a unique and novel mechanism of action, that defines a new and drugable cellular route that links PPARs to Akt/mTORC1 axis, where TRIB3 pseudokinase plays a central role. Activation of this route (PPARα/γ-TRIB3-Akt-mTORC1) leads to autophagy-mediated cancer cell death. Given the low toxicity and high tolerability of ABTL0812, our results support further development of ABTL0812 as a promising anticancer therapy. Clin Cancer Res; 22(10); 2508-19. ©2015 AACR., (©2015 American Association for Cancer Research.)
- Published
- 2016
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15. MicroRNA-497 impairs the growth of chemoresistant neuroblastoma cells by targeting cell cycle, survival and vascular permeability genes.
- Author
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Soriano A, París-Coderch L, Jubierre L, Martínez A, Zhou X, Piskareva O, Bray I, Vidal I, Almazán-Moga A, Molist C, Roma J, Bayascas JR, Casanovas O, Stallings RL, Sánchez de Toledo J, Gallego S, and Segura MF
- Subjects
- Animals, Apoptosis genetics, Cell Line, Tumor, Cell Proliferation genetics, Doxycycline therapeutic use, Drug Resistance, Neoplasm, Female, Gene Expression Regulation, Neoplastic genetics, Humans, Mice, Mice, Nude, MicroRNAs biosynthesis, Neuroblastoma drug therapy, Neuroblastoma mortality, Treatment Outcome, Xenograft Model Antitumor Assays, Capillary Permeability genetics, Cell Cycle genetics, Cell Survival genetics, MicroRNAs genetics, Neovascularization, Pathologic genetics, Neuroblastoma genetics, Neuroblastoma pathology
- Abstract
Despite multimodal therapies, a high percentage of high-risk neuroblastoma (NB) become refractory to current treatments, most of which interfere with cell cycle and DNA synthesis or function, activating the DNA damage response (DDR). In cancer, this process is frequently altered by deregulated expression or function of several genes which contribute to multidrug resistance (MDR). MicroRNAs are outstanding candidates for therapy since a single microRNA can modulate the expression of multiple genes of the same or different pathways, thus hindering the development of resistance mechanisms by the tumor. We found several genes implicated in the MDR to be overexpressed in high-risk NB which could be targeted by microRNAs simultaneously. Our functional screening identified several of those microRNAs that reduced proliferation of chemoresistant NB cell lines, the best of which was miR-497. Low expression of miR-497 correlated with poor patient outcome. The overexpression of miR-497 reduced the proliferation of multiple chemoresistant NB cell lines and induced apoptosis in MYCN-amplified cell lines. Moreover, the conditional expression of miR-497 in NB xenografts reduced tumor growth and inhibited vascular permeabilization. MiR-497 targets multiple genes related to the DDR, cell cycle, survival and angiogenesis, which renders this molecule a promising candidate for NB therapy.
- Published
- 2016
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16. Fine-tuning the intensity of the PKB/Akt signal enables diverse physiological responses.
- Author
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Zhou X, Cordon-Barris L, Zurashvili T, and Bayascas JR
- Subjects
- Animals, Mice, Mice, Mutant Strains, Neurons metabolism, Phosphatidylinositols metabolism, Phosphatidylinositol 3-Kinases metabolism, Proto-Oncogene Proteins c-akt metabolism
- Abstract
The PI3K/PDK1/PKB signaling pathway plays essential roles in regulating neuronal survival, differentiation and plasticity in response to neurotrophic factors, neurotransmitters and ion channels. Both PDK1 and PKB can interact at the plasma membrane with a phosphoinositide synthesized by PI3K, the second messenger PtdIns(3,4,5)P3, enabling PDK1 to phosphorylate and activate PKB. In the PDK1 K465E knock-in mice expressing a mutant form of PDK1 incapable of phosphoinositide binding, activation of PKB was markedly affected, but not totally abolished. It has been recently proposed that in the absence of PtdIns(3,4,5)P3 binding, PDK1 can still moderately activate PKB due to a docking site-mediated interaction of these 2 kinases. A recent report has uncovered that in the PDK1 K465E mice neurons, a PKB signal threshold was sufficient to support neuronal survival responses, whereas neuritogenesis, neuronal polarization and axon outgrowth were severely impaired. We propose here that the low-efficiency mechanism of PKB activation observed in the PDK1 K465E mice might represent the ancestral mechanism responsible for the essential functions of this pathway, while the phosphoinositide-dependent activation should be considered an evolutionary innovation that enabled the acquisition of novel functions.
- Published
- 2014
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17. Canonical and kinase activity-independent mechanisms for extracellular signal-regulated kinase 5 (ERK5) nuclear translocation require dissociation of Hsp90 from the ERK5-Cdc37 complex.
- Author
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Erazo T, Moreno A, Ruiz-Babot G, Rodríguez-Asiain A, Morrice NA, Espadamala J, Bayascas JR, Gómez N, and Lizcano JM
- Subjects
- Animals, Cell Cycle Proteins biosynthesis, Cell Cycle Proteins genetics, Cell Line, Cell Proliferation, Chaperonins biosynthesis, Chaperonins genetics, HEK293 Cells, HeLa Cells, Humans, Mice, Mitogen-Activated Protein Kinase 7 genetics, Phosphorylation, RNA Interference, RNA, Small Interfering, Signal Transduction, Transcription, Genetic, Transcriptional Activation, Ubiquitination, Active Transport, Cell Nucleus, Cell Cycle Proteins metabolism, Cell Nucleus metabolism, Chaperonins metabolism, HSP90 Heat-Shock Proteins metabolism, Mitogen-Activated Protein Kinase 7 metabolism
- Abstract
The mitogen-activated protein (MAP) kinase extracellular signal-regulated kinase 5 (ERK5) plays a crucial role in cell proliferation, regulating gene transcription. ERK5 has a unique C-terminal tail which contains a transcriptional activation domain, and activates transcription by phosphorylating transcription factors and acting itself as a transcriptional coactivator. However, the molecular mechanisms that regulate its nucleocytoplasmatic traffic are unknown. We have used tandem affinity purification to identify proteins that interact with ERK5. We show that ERK5 interacts with the Hsp90-Cdc37 chaperone in resting cells, and that inhibition of Hsp90 or Cdc37 results in ERK5 ubiquitylation and proteasomal degradation. Interestingly, activation of cellular ERK5 induces Hsp90 dissociation from the ERK5-Cdc37 complex, leading to ERK5 nuclear translocation and activation of transcription, by a mechanism which requires the autophosphorylation at its C-terminal tail. Consequently, active ERK5 is no longer sensitive to Hsp90 or Cdc37 inhibitors. Cdc37 overexpression also induces Hsp90 dissociation and the nuclear translocation of a kinase-inactive form of ERK5 which retains transcriptional activity. This is the first example showing that ERK5 transcriptional activity does not require kinase activity. Since Cdc37 cooperates with ERK5 to promote cell proliferation, Cdc37 overexpression (as happens in some cancers) might represent a new, noncanonical mechanism by which ERK5 regulates tumor proliferation.
- Published
- 2013
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18. Interaction of PDK1 with phosphoinositides is essential for neuronal differentiation but dispensable for neuronal survival.
- Author
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Zurashvili T, Cordón-Barris L, Ruiz-Babot G, Zhou X, Lizcano JM, Gómez N, Giménez-Llort L, and Bayascas JR
- Subjects
- 3-Phosphoinositide-Dependent Protein Kinases, Animals, Brain anatomy & histology, Brain cytology, Brain-Derived Neurotrophic Factor metabolism, Cell Survival, Cells, Cultured, Enzyme Activation, Gene Knock-In Techniques, Humans, Mice, Mutation, Neurons metabolism, Organ Size, Protein Binding, Protein Serine-Threonine Kinases chemistry, Protein Serine-Threonine Kinases genetics, Protein Structure, Tertiary, Proto-Oncogene Proteins c-akt metabolism, Ribosomal Protein S6 Kinases metabolism, Neurogenesis, Neurons cytology, Phosphatidylinositol Phosphates metabolism, Protein Serine-Threonine Kinases metabolism
- Abstract
3-Phosphoinositide-dependent protein kinase 1 (PDK1) operates in cells in response to phosphoinositide 3-kinase activation and phosphatidylinositol-3,4,5-trisphosphate [PtdIns(3,4,5)P(3)] production by activating a number of AGC kinases, including protein kinase B (PKB)/Akt. Both PDK1 and PKB contain pleckstrin homology (PH) domains that interact with the PtdIns(3,4,5)P(3) second messenger. Disrupting the interaction of the PDK1 PH domain with phosphoinositides by expressing the PDK1 K465E knock-in mutation resulted in mice with reduced PKB activation. We explored the physiological consequences of this biochemical lesion in the central nervous system. The PDK1 knock-in mice displayed a reduced brain size due to a reduction in neuronal cell size rather than cell number. Reduced BDNF-induced phosphorylation of PKB at Thr308, the PDK1 site, was observed in the mutant neurons, which was not rate limiting for the phosphorylation of those PKB substrates governing neuronal survival and apoptosis, such as FOXO1 or glycogen synthase kinase 3 (GSK3). Accordingly, the integrity of the PDK1 PH domain was not essential to support the survival of different embryonic neuronal populations analyzed. In contrast, PKB-mediated phosphorylation of PRAS40 and TSC2, allowing optimal mTORC1 activation and brain-specific kinase (BRSK) protein synthesis, was markedly reduced in the mutant mice, leading to impaired neuronal growth and differentiation.
- Published
- 2013
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19. Brain specific kinase-1 BRSK1/SAD-B associates with lipid rafts: modulation of kinase activity by lipid environment.
- Author
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Rodríguez-Asiain A, Ruiz-Babot G, Romero W, Cubí R, Erazo T, Biondi RM, Bayascas JR, Aguilera J, Gómez N, Gil C, Claro E, and Lizcano JM
- Subjects
- AMP-Activated Protein Kinase Kinases, Animals, Baculoviridae, Escherichia coli, Fetus, HEK293 Cells, Humans, Lipoylation, Membranes, Artificial, Mice, Phosphorylation, Protein Structure, Secondary, Rats, Rats, Sprague-Dawley, Recombinant Proteins genetics, Threonine metabolism, Brain physiology, Intracellular Signaling Peptides and Proteins metabolism, Membrane Microdomains metabolism, Protein Serine-Threonine Kinases metabolism, Recombinant Proteins metabolism, Signal Transduction physiology, Synaptic Transmission physiology, Synaptosomes metabolism
- Abstract
Brain specific kinases 1 and 2 (BRSK1/2, also named SAD kinases) are serine-threonine kinases specifically expressed in the brain, and activated by LKB1-mediated phosphorylation of a threonine residue at their T-loop (Thr189/174 in human BRSK1/2). BRSKs are crucial for establishing neuronal polarity, and BRSK1 has also been shown to regulate neurotransmitter release presynaptically. How BRSK1 exerts this latter function is unknown, since its substrates at the synaptic terminal and the mechanisms modulating its activity remain to be described. Key regulators of neurotransmitter release, such as SNARE complex proteins, are located at membrane rafts. Therefore we initially undertook this work to check whether BRSK1 also locates at these membrane microdomains. Here we show that brain BRSK1, but not BRSK2, is palmitoylated, and provide biochemical and pharmacological evidences demonstrating that a pool of BRSK1, but not BRSK2 or LKB1, localizes at membrane lipid rafts. We also show that raft-associated BRSK1 has higher activity than BRSK1 from non-raft environment, based on a higher T-loop phosphorylation at Thr-189. Further, recombinant BRSK1 activity increased 3-fold when assayed with small multilamellar vesicles (SMV) generated with lipids extracted from synaptosomal raft fractions. A similar BRSK1-activating effect was obtained with synthetic SMV made with phosphatidylcholine, cholesterol and sphingomyelin, mixed in the same molar ratio at which these three major lipids are present in rafts. Importantly, SMV also enhanced the activity of a constitutively active BRSK1 (T189E), underpinning that interaction with lipid rafts represents a new mechanism of BRSK1 activity modulation, additional to T-loop phosphorylation., (Copyright © 2011 Elsevier B.V. All rights reserved.)
- Published
- 2011
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20. Activation of the cardiac mTOR/p70(S6K) pathway by leucine requires PDK1 and correlates with PRAS40 phosphorylation.
- Author
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Sanchez Canedo C, Demeulder B, Ginion A, Bayascas JR, Balligand JL, Alessi DR, Vanoverschelde JL, Beauloye C, Hue L, and Bertrand L
- Subjects
- 3-Phosphoinositide-Dependent Protein Kinases, Animals, Blotting, Western, Enzyme Activation physiology, Glutamine physiology, Heart physiology, Hypoglycemic Agents pharmacology, In Vitro Techniques, Insulin pharmacology, Mice, Mice, Inbred C57BL, Mice, Knockout, Myocardium metabolism, Phenylalanine metabolism, Phosphatidylinositol 3-Kinases physiology, Phosphorylation, Protein Serine-Threonine Kinases genetics, Protein Serine-Threonine Kinases metabolism, Rats, Ribosomal Protein S6 Kinases, 70-kDa genetics, Signal Transduction drug effects, TOR Serine-Threonine Kinases, Threonine physiology, Heart drug effects, Intracellular Signaling Peptides and Proteins physiology, Leucine pharmacology, Phosphoproteins metabolism, Protein Serine-Threonine Kinases physiology, Ribosomal Protein S6 Kinases, 70-kDa physiology
- Abstract
Like insulin, leucine stimulates the mammalian target of rapamycin (mTOR)/p70 ribosomal S6 kinase (p70(S6K)) axis in various organs. Insulin proceeds via the canonical association of phosphatidylinositol 3-kinase (PI3K), phosphoinositide-dependent protein kinase-1 (PDK1), and protein kinase B (PKB/Akt). The signaling involved in leucine effect, although known to implicate a PI3K mechanism independent of PKB/Akt, is more poorly understood. In this study, we investigated whether PDK1 could also participate in the events leading to mTOR/p70(S6K) activation in response to leucine in the heart. In wild-type hearts, both leucine and insulin increased p70(S6K) activity whereas, in contrast to insulin, leucine was unable to activate PKB/Akt. The changes in p70(S6K) activity induced by insulin and leucine correlated with changes in phosphorylation of Thr(389), the mTOR phosphorylation site on p70(S6K), and of Ser(2448) on mTOR, both related to mTOR activity. Leucine also triggered phosphorylation of the proline-rich Akt/PKB substrate of 40 kDa (PRAS40), a new pivotal mTOR regulator. In PDK1 knockout hearts, leucine, similarly to insulin, failed to induce the phosphorylation of mTOR and p70(S6K), leading to the absence of p70(S6K) activation. The loss of leucine effect in absence of PDK1 correlated with the lack of PRAS40 phosphorylation. Moreover, the introduction in PDK1 of the L155E mutation, which is known to preserve the insulin-induced and PKB/Akt-dependent phosphorylation of mTOR/p70(S6K), suppressed all leucine effects, including phosphorylation of mTOR, PRAS40, and p70(S6K). We conclude that the leucine-induced stimulation of the cardiac PRAS40/mTOR/p70(S6K) pathway requires PDK1 in a way that differs from that of insulin.
- Published
- 2010
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21. PDK1: the major transducer of PI 3-kinase actions.
- Author
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Bayascas JR
- Subjects
- 3-Phosphoinositide-Dependent Protein Kinases, Animals, Humans, Protein Serine-Threonine Kinases antagonists & inhibitors, Protein Serine-Threonine Kinases chemistry, Transcription Factors physiology, Phosphatidylinositol 3-Kinases physiology, Protein Serine-Threonine Kinases physiology
- Abstract
Most of the cellular responses to phosphatidylinositol 3-kinase activation and phosphatidylinositol 3,4,5-trisphosphate production are mediated by the activation of a group of AGC kinases comprising PKB, S6K, RSK, SGK and PKC isoforms, which play essential roles in regulating physiological processes related to cell growth, proliferation, survival and metabolism. All these growth-factor-stimulated AGC kinases possess a common upstream activator, namely PDK1, a master kinase, which, being constitutively active, is still able to phosphorylate and activate its AGC substrates in response to rises in the levels of the PtdIns(3,4,5)P(3) second messenger. In this chapter, the biochemical, structural and genetic data on the mechanism of action and physiological roles of PDK1 are reviewed, and its potential as a pharmaceutical target for the design of drugs therapeutically beneficial to treat human disease such us diabetes and cancer is discussed.
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- 2010
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22. Phosphoinositide (3,4,5)-triphosphate binding to phosphoinositide-dependent kinase 1 regulates a protein kinase B/Akt signaling threshold that dictates T-cell migration, not proliferation.
- Author
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Waugh C, Sinclair L, Finlay D, Bayascas JR, and Cantrell D
- Subjects
- 3-Phosphoinositide-Dependent Protein Kinases, Animals, Cell Differentiation, Cell Proliferation, Cell Survival, Cytotoxicity, Immunologic, Enzyme Activation, Forkhead Transcription Factors metabolism, Lymphocyte Activation, Mice, Models, Biological, Phosphorylation, Receptors, Lymphocyte Homing metabolism, Cell Movement, Phosphatidylinositols metabolism, Protein Serine-Threonine Kinases metabolism, Proto-Oncogene Proteins c-akt metabolism, Signal Transduction, T-Lymphocytes cytology, T-Lymphocytes enzymology
- Abstract
The present study explored the consequences of phosphoinositide (3,4,5)-triphosphate [PI(3,4,5)P(3)] binding to the pleckstrin homology (PH) domain of the serine/threonine kinase 3-phosphoinositide-dependent kinase 1 (PDK1). The salient finding is that PDK1 directly transduces the PI(3,4,5)P(3) signaling that determines T-cell trafficking programs but not T-cell growth and proliferation. The integrity of the PDK1 PH domain thus is not required for PDK1 catalytic activity or to support cell survival and the proliferation of thymic and peripheral T cells. However, a PDK1 mutant that cannot bind PI(3,4,5)P(3) cannot trigger the signals that terminate the expression of the transcription factor KLF2 in activated T cells and cannot switch the chemokine and adhesion receptor profile of naive T cells to the profile of effector T cells. The PDK1 PH domain also is required for the maximal activation of Akt/protein kinase B (PKB) and for the maximal phosphorylation and inactivation of Foxo family transcription factors in T cells. PI(3,4,5)P(3) binding to PDK1 and the strength of PKB activity thus can dictate the nature of the T-cell response. Low levels of PKB activity can be sufficient for T-cell proliferation but insufficient to initiate the migratory program of effector T cells.
- Published
- 2009
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23. Dissecting the role of the 3-phosphoinositide-dependent protein kinase-1 (PDK1) signalling pathways.
- Author
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Bayascas JR
- Subjects
- 3-Phosphoinositide-Dependent Protein Kinases, Animals, Binding Sites, Insulin Resistance, Mice, Phenotype, Phosphatidylinositol Phosphates metabolism, Protein Isoforms genetics, Protein Isoforms metabolism, Protein Serine-Threonine Kinases genetics, Protein Structure, Tertiary, Protein Serine-Threonine Kinases metabolism, Signal Transduction
- Abstract
The 3-phosphoinositide-dependent protein kinase-1 (PDK1) mediates the cellular effect of insulin and growth factors by activating a group of kinases including PKB/Akt, S6K, RSK, SGK and PKC isoforms. PDK1 possesses two regulatory domains namely a Pleckstrin Homology (PH) domain that binds to the phosphatidylinositol 3,4,5-trisphosphate [PtdIns(3,4,5)P(3)] second messenger, and a substrate binding site termed the PIF-pocket. Employing a combination of biochemical, structural and mouse knock-in approaches we have been able to define the roles that the regulatory domains on PDK1 play. We have established that binding of PDK1 to PtdIns(3,4,5)P(3) is essential for efficient activation of PKB isoforms as well as for maintaining normal cell size and insulin sensitivity. In contrast, the PIF-substrate binding pocket of PDK1 is not required for PKB activation, but is necessary for PDK1 to activate all of its other substrates.
- Published
- 2008
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24. Mutation of the PDK1 PH domain inhibits protein kinase B/Akt, leading to small size and insulin resistance.
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Bayascas JR, Wullschleger S, Sakamoto K, García-Martínez JM, Clacher C, Komander D, van Aalten DM, Boini KM, Lang F, Lipina C, Logie L, Sutherland C, Chudek JA, van Diepen JA, Voshol PJ, Lucocq JM, and Alessi DR
- Subjects
- Amino Acid Substitution, Animals, Body Size physiology, Female, Insulin Resistance physiology, Male, Mice, Mice, Inbred C57BL, Mice, Mutant Strains, Models, Molecular, Mutagenesis, Site-Directed, Phenotype, Prediabetic State genetics, Prediabetic State metabolism, Protein Serine-Threonine Kinases chemistry, Protein Structure, Tertiary, Pyruvate Dehydrogenase Acetyl-Transferring Kinase, Body Size genetics, Insulin Resistance genetics, Mutation, Protein Serine-Threonine Kinases genetics, Protein Serine-Threonine Kinases metabolism, Proto-Oncogene Proteins c-akt antagonists & inhibitors
- Abstract
PDK1 activates a group of kinases, including protein kinase B (PKB)/Akt, p70 ribosomal S6 kinase (S6K), and serum and glucocorticoid-induced protein kinase (SGK), that mediate many of the effects of insulin as well as other agonists. PDK1 interacts with phosphoinositides through a pleckstrin homology (PH) domain. To study the role of this interaction, we generated knock-in mice expressing a mutant of PDK1 incapable of binding phosphoinositides. The knock-in mice are significantly small, insulin resistant, and hyperinsulinemic. Activation of PKB is markedly reduced in knock-in mice as a result of lower phosphorylation of PKB at Thr308, the residue phosphorylated by PDK1. This results in the inhibition of the downstream mTOR complex 1 and S6K1 signaling pathways. In contrast, activation of SGK1 or p90 ribosomal S6 kinase or stimulation of S6K1 induced by feeding is unaffected by the PDK1 PH domain mutation. These observations establish the importance of the PDK1-phosphoinositide interaction in enabling PKB to be efficiently activated with an animal model. Our findings reveal how reduced activation of PKB isoforms impinges on downstream signaling pathways, causing diminution of size as well as insulin resistance.
- Published
- 2008
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25. The long form of Fas apoptotic inhibitory molecule is expressed specifically in neurons and protects them against death receptor-triggered apoptosis.
- Author
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Segura MF, Sole C, Pascual M, Moubarak RS, Perez-Garcia MJ, Gozzelino R, Iglesias V, Badiola N, Bayascas JR, Llecha N, Rodriguez-Alvarez J, Soriano E, Yuste VJ, and Comella JX
- Subjects
- Animals, Apoptosis genetics, Apoptosis Regulatory Proteins physiology, Cells, Cultured, Gene Expression Regulation physiology, Genetic Variation physiology, Humans, Mice, Neurons pathology, PC12 Cells, Protein Isoforms biosynthesis, Protein Isoforms genetics, Protein Isoforms physiology, Rats, Receptors, Death Domain genetics, Apoptosis physiology, Apoptosis Regulatory Proteins biosynthesis, Apoptosis Regulatory Proteins genetics, Inhibitor of Apoptosis Proteins physiology, Neurons metabolism, Receptors, Death Domain antagonists & inhibitors, Receptors, Death Domain physiology
- Abstract
Death receptors (DRs) and their ligands are expressed in developing nervous system. However, neurons are generally resistant to death induction through DRs and rather their activation promotes neuronal outgrowth and branching. These results suppose the existence of DRs antagonists expressed in the nervous system. Fas apoptosis inhibitory molecule (FAIM(S)) was first identified as a Fas antagonist in B-cells. Soon after, a longer alternative spliced isoform with unknown function was identified and named FAIM(L). FAIM(S) is widely expressed, including the nervous system, and we have shown previously that it promotes neuronal differentiation but it is not an anti-apoptotic molecule in this system. Here, we demonstrate that FAIM(L) is expressed specifically in neurons, and its expression is regulated during the development. Expression could be induced by NGF through the extracellular regulated kinase pathway in PC12 (pheochromocytoma cell line) cells. Contrary to FAIM(S), FAIM(L) does not increase the neurite outgrowth induced by neurotrophins and does not interfere with nuclear factor kappaB pathway activation as FAIM(S) does. Cells overexpressing FAIM(L) are resistant to apoptotic cell death induced by DRs such as Fas or tumor necrosis factor R1. Reduction of endogenous expression by small interfering RNA shows that endogenous FAIM(L) protects primary neurons from DR-induced cell death. The detailed analysis of this antagonism shows that FAIM(L) can bind to Fas receptor and prevent the activation of the initiator caspase-8 induced by Fas. In conclusion, our results indicate that FAIM(L) could be responsible for maintaining initiator caspases inactive after receptor engagement protecting neurons from the cytotoxic action of death ligands.
- Published
- 2007
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26. Evaluation of approaches to generation of tissue-specific knock-in mice.
- Author
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Bayascas JR, Sakamoto K, Armit L, Arthur JS, and Alessi DR
- Subjects
- 3-Phosphoinositide-Dependent Protein Kinases, Animals, Biological Transport, Genetic Vectors, Glucose metabolism, Heterozygote, Insulin metabolism, Mice, Mice, Transgenic, Phenotype, Protein Binding, Protein Serine-Threonine Kinases metabolism, Proto-Oncogene Proteins c-akt metabolism, Ribosomal Protein S6 Kinases, 70-kDa metabolism, Genetic Techniques
- Abstract
We explored three approaches to create tissue-specific knock-in mice by generating knock-in mice in which a substrate-docking site of the PDK1 protein kinase was ablated in Cre-expressing tissues in a way that prevented activation of one of its substrates, p70 ribosomal S6 kinase (S6K), but not another (protein kinase B (PKB)). Employing two of the approaches, termed the "heterozygous" and "minigene" methods, we generated mice in which Cre-expressing skeletal and cardiac muscle produced the mutant rather than wild type PDK1. Consistent with this, injection of these mice with insulin only induced activation of PKB but not S6K in muscle tissues. We have also demonstrated that insulin-stimulated glucose uptake proceeds normally in knock-in mice, consistent with the notion that PKB mediates this process. In contrast to conditional knock-out of PDK1 in muscle, the knock-in mice did not develop dilated cardiomyopathy, suggesting that PKB plays a key role in protecting mice from heart failure. The third knock-in strategy that was evaluated, termed the "inversion" method, did not proceed with high efficiency. We discuss the merits and disadvantages of each of the conditional knock-in approaches, along with the applications for which they may be most suited, and suggest how they could be further refined.
- Published
- 2006
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27. LKB1-dependent signaling pathways.
- Author
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Alessi DR, Sakamoto K, and Bayascas JR
- Subjects
- AMP-Activated Protein Kinase Kinases, AMP-Activated Protein Kinases, Adaptor Proteins, Vesicular Transport genetics, Adaptor Proteins, Vesicular Transport metabolism, Amino Acid Sequence, Animals, Cell Polarity, Enzyme Activation, Humans, Isoenzymes classification, Isoenzymes genetics, Isoenzymes metabolism, Molecular Sequence Data, Multienzyme Complexes classification, Multienzyme Complexes genetics, Multienzyme Complexes metabolism, Mutation, Phylogeny, Protein Processing, Post-Translational, Protein Serine-Threonine Kinases classification, Protein Serine-Threonine Kinases genetics, Sequence Alignment, Genes, Tumor Suppressor, Peutz-Jeghers Syndrome genetics, Peutz-Jeghers Syndrome metabolism, Peutz-Jeghers Syndrome therapy, Protein Serine-Threonine Kinases metabolism, Signal Transduction physiology
- Abstract
This review focuses on remarkable recent findings concerning the mechanism by which the LKB1 protein kinase that is mutated in Peutz-Jeghers cancer syndrome operates as a tumor suppressor. We discuss evidence that the cellular localization and activity of LKB1 is controlled through its interaction with a catalytically inactive protein resembling a protein kinase, termed STRAD, and an armadillo repeat-containing protein, named mouse protein 25 (MO25). The data suggest that LKB1 functions as a tumor suppressor by not only inhibiting proliferation, but also by exerting profound effects on cell polarity and, most unexpectedly, on the ability of a cell to detect and respond to low cellular energy levels. Genetic and biochemical findings indicate that LKB1 exerts its effects by phosphorylating and activating 14 protein kinases, all related to the AMP-activated protein kinase. The work described in this review shows how a study of an obscure cancer syndrome can uncover new and important regulatory pathways, relevant to the understanding of multiple human diseases.
- Published
- 2006
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28. Hypomorphic mutation of PDK1 suppresses tumorigenesis in PTEN(+/-) mice.
- Author
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Bayascas JR, Leslie NR, Parsons R, Fleming S, and Alessi DR
- Subjects
- 3-Phosphoinositide-Dependent Protein Kinases, Age Factors, Animals, Immunohistochemistry, Mice, Mice, Mutant Strains, Mutation genetics, Neoplasms enzymology, Neoplasms pathology, Ribosomal Protein S6 Kinases metabolism, Survival Analysis, Neoplasms genetics, PTEN Phosphohydrolase genetics, Protein Serine-Threonine Kinases genetics, Protein Serine-Threonine Kinases metabolism, Signal Transduction genetics
- Abstract
Many cancers possess elevated levels of PtdIns(3,4,5)P(3), the second messenger that induces activation of the protein kinases PKB/Akt and S6K and thereby stimulates cell proliferation, growth, and survival. The importance of this pathway in tumorigenesis has been highlighted by the finding that PTEN, the lipid phosphatase that breaks down PtdIns(3,4,5)P(3) to PtdIns(4,5)P(2), is frequently mutated in human cancer. Cells lacking PTEN possess elevated levels of PtdIns(3,4,5)P(3), PKB, and S6K activity and heterozygous PTEN(+/-) mice develop a variety of tumors. Knockout of PKBalpha in PTEN-deficient cells reduces aggressive growth and promotes apoptosis, whereas treatment of PTEN(+/-) mice with rapamycin, an inhibitor of the activation of S6K, reduces neoplasia. We explored the importance of PDK1, the protein kinase that activates PKB and S6K, in mediating tumorigenesis caused by the deletion of PTEN. We demonstrate that reducing the expression of PDK1 in PTEN(+/-) mice, markedly protects these animals from developing a wide range of tumors. Our findings provide genetic evidence that PDK1 is a key effector in mediating neoplasia resulting from loss of PTEN and also validate PDK1 as a promising anticancer target for the prevention of tumors that possess elevated PKB and S6K activity.
- Published
- 2005
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29. The contribution of apoptosis-inducing factor, caspase-activated DNase, and inhibitor of caspase-activated DNase to the nuclear phenotype and DNA degradation during apoptosis.
- Author
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Yuste VJ, Sánchez-López I, Solé C, Moubarak RS, Bayascas JR, Dolcet X, Encinas M, Susin SA, and Comella JX
- Subjects
- Apoptosis, Blotting, Western, Cell Line, Tumor, DNA Fragmentation, Deoxyribonucleases chemistry, Dose-Response Relationship, Drug, Enzyme Inhibitors pharmacology, Humans, In Situ Nick-End Labeling, Microscopy, Electron, Microscopy, Fluorescence, Molecular Weight, Mutagenesis, Phenotype, Plasmids metabolism, Staurosporine pharmacology, Transfection, Apoptosis Inducing Factor physiology, Cell Nucleus metabolism, DNA chemistry, Deoxyribonucleases antagonists & inhibitors, Deoxyribonucleases physiology
- Abstract
We have assessed the contribution of apoptosis-inducing factor (AIF) and inhibitor of caspase-activated DNase (ICAD) to the nuclear morphology and DNA degradation pattern in staurosporine-induced apoptosis. Expression of D117E ICAD, a mutant that is resistant to caspase cleavage at residue 117, prevented low molecular weight (LMW) DNA fragmentation, stage II nuclear morphology, and detection of terminal deoxynucleotidyl transferase staining. However, high molecular weight (HMW) DNA fragmentation and stage I nuclear morphology remained unaffected. On the other hand, expression of either D224E or wild type ICAD had no effect on DNA fragmentation or nuclear morphology. In addition, both HMW and LMW DNA degradation required functional executor caspases. Interestingly, silencing of endogenous AIF abolished type I nuclear morphology without any effect on HMW or LMW DNA fragmentation. Together, these results demonstrate that AIF is responsible for stage I nuclear morphology and suggest that HMW DNA degradation is a caspase-activated DNase and AIF-independent process.
- Published
- 2005
- Full Text
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30. Regulation of Akt/PKB Ser473 phosphorylation.
- Author
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Bayascas JR and Alessi DR
- Subjects
- Acid Phosphatase chemistry, Acid Phosphatase genetics, Enzyme Activation, Models, Biological, Phosphorylation, Protein Serine-Threonine Kinases genetics, Protein Structure, Tertiary, Proto-Oncogene Proteins genetics, Proto-Oncogene Proteins c-akt, Acid Phosphatase metabolism, Gene Expression Regulation, Enzymologic, Protein Serine-Threonine Kinases metabolism, Proto-Oncogene Proteins metabolism, Serine metabolism
- Abstract
For years the Akt/PKB research field has been in turmoil, trying to understand how the activating phosphorylation of Akt/PKB at Ser473 is regulated. In the past month, papers in a recent issue of Molecular Cell (Gao et al., 2005) and in Science (Sarbassov et al., 2005) may have identified the phosphatase and kinase acting on this residue.
- Published
- 2005
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31. The death receptor antagonist FAIM promotes neurite outgrowth by a mechanism that depends on ERK and NF-kapp B signaling.
- Author
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Sole C, Dolcet X, Segura MF, Gutierrez H, Diaz-Meco MT, Gozzelino R, Sanchis D, Bayascas JR, Gallego C, Moscat J, Davies AM, and Comella JX
- Subjects
- Animals, Apoptosis Regulatory Proteins, Humans, Mice, NF-kappa B metabolism, Neurons cytology, Oncogene Proteins metabolism, PC12 Cells, Proteins genetics, Proteins metabolism, RNA, Small Interfering pharmacology, Rats, Receptor, Nerve Growth Factor, Receptors, Nerve Growth Factor metabolism, Signal Transduction, Transfection, MAP Kinase Signaling System, NF-kappa B physiology, Neurites metabolism, Proteins physiology
- Abstract
Fas apoptosis inhibitory molecule (FAIM) is a protein identified as an antagonist of Fas-induced cell death. We show that FAIM overexpression fails to rescue neurons from trophic factor deprivation, but exerts a marked neurite growth-promoting action in different neuronal systems. Whereas FAIM overexpression greatly enhanced neurite outgrowth from PC12 cells and sympathetic neurons grown with nerve growth factor (NGF), reduction of endogenous FAIM levels by RNAi decreased neurite outgrowth in these cells. FAIM overexpression promoted NF-kappa B activation, and blocking this activation by using a super-repressor I kappa B alpha or by carrying out experiments using cortical neurons from mice that lack the p65 NF-kappa B subunit prevented FAIM-induced neurite outgrowth. The effect of FAIM on neurite outgrowth was also blocked by inhibition of the Ras-ERK pathway. Finally, we show that FAIM interacts with both Trk and p75 neurotrophin receptor NGF receptors in a ligand-dependent manner. These results reveal a new function of FAIM in promoting neurite outgrowth by a mechanism involving activation of the Ras-ERK pathway and NF-kappa B.
- Published
- 2004
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32. Characterization of splice variants of human caspase-activated DNase with CIDE-N structure and function.
- Author
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Bayascas JR, Yuste VJ, Solé C, Sánchez-López I, Segura MF, Perera R, and Comella JX
- Subjects
- Alternative Splicing, Amino Acid Sequence, Animals, Apoptosis, Apoptosis Regulatory Proteins, Base Sequence, Caspases metabolism, Cell Line, DNA Fragmentation, Deoxyribonucleases chemistry, Deoxyribonucleases genetics, Humans, Mice, Molecular Sequence Data, Neuroblastoma metabolism, Peptide Fragments chemistry, Peptide Fragments genetics, Peptide Fragments metabolism, Protein Isoforms, Protein Structure, Tertiary, Proteins chemistry, Proteins genetics, Recombinant Proteins chemistry, Recombinant Proteins genetics, Recombinant Proteins metabolism, Tissue Distribution, Transcription, Genetic, Deoxyribonucleases metabolism, Proteins metabolism
- Abstract
Internucleosomal DNA fragmentation is an apoptotic event that depends on the activity of different nucleases. Among them, the DNA fragmentation factor B, better known as caspase-activated DNase (CAD), is mainly responsible for this DNA fragmentation in dying cells. CAD is an endonuclease that is chaperoned and inhibited by inhibitor of CAD (ICAD). Activation of CAD needs the cleavage of ICAD by activated caspase-3. During the characterization of the staurosporine-induced apoptotic process in human neuroblastoma cell lines, we have found three novel splice variants of CAD. In all three messengers, the open reading frame is truncated after the second exon of the CAD gene. This truncated open reading frame codifies the CAD protein amino terminal part corresponding to the cell death-inducing DFF45-like effector-N (CIDE-N) domain. We have detected these splicing variants in human tissues and in peripheral white blood cells from 10 unrelated individuals, and their products have been showed to be expressed in certain mouse tissues. We demonstrate that these truncated forms of CAD are soluble proteins that interact with ICAD. We also provided evidences that these CIDE-N forms of CAD promote apoptosis in a caspase-dependent manner.
- Published
- 2004
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33. Isolation of AmphiCASP-3/7, an ancestral caspase from amphioxus (Branchiostoma floridae). Evolutionary considerations for vertebrate caspases.
- Author
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Bayascas JR, Yuste VJ, Benito E, Garcia-Fernàndez J, and Comella JX
- Subjects
- Animals, Caspase 3, Caspase 7, Caspases deficiency, Caspases genetics, Chordata, Nonvertebrate embryology, Chordata, Nonvertebrate growth & development, DNA, Complementary analysis, DNA, Complementary genetics, Embryo, Mammalian cytology, Embryo, Mammalian embryology, Embryo, Mammalian enzymology, Embryo, Nonmammalian, Evolution, Molecular, Female, Gene Expression Regulation, Enzymologic physiology, Humans, Larva cytology, Larva enzymology, Larva growth & development, Male, Molecular Sequence Data, Phenotype, Phylogeny, Sequence Homology, Amino Acid, Sequence Homology, Nucleic Acid, Tumor Cells, Cultured, Apoptosis genetics, Caspases isolation & purification, Chordata, Nonvertebrate enzymology
- Abstract
Caspases are a large family of cysteine proteases that play an essential role as effectors of apoptosis in metazoans. Thirteen different caspases have been identified in vertebrates so far, and their function in apoptotic or inflammatory responses is well documented. We have taken advantage of the broadly accepted condition of amphioxus (Cephalochordata, Branchiostoma floridae) as the closest living relative to vertebrates to study the molecular evolution of caspases. Here we report for the first time the pattern of programmed cell death during development of cephalochordates. We also describe the isolation and functional characterisation of the first caspase related gene in amphioxus, which we named AmphiCASP-3/7. The amphioxus caspase is expressed throughout development, from the gastrula to larva stage. AmphiCASP-3/7 induced cell death when ectopically expressed in human HEK 293T cells, and the recombinant protein was inhibited by DEVD peptides. AmphiCASP-3/7 reflects the primitive condition of the executor vertebrates caspases -3 and -7, prior to vertebrate specific duplication. Interestingly, AmphiCASP-3/7 is functionally closer to vertebrate caspase-7, as shown by substrate specificity both in vitro and in MCF7 cells. Our phylogenetic and functional data help in drawing the evolutionary history of caspases, and illustrates an example of acquisition in vertebrates of novel functional properties after gene duplication.
- Published
- 2002
- Full Text
- View/download PDF
34. The prevention of the staurosporine-induced apoptosis by Bcl-X(L), but not by Bcl-2 or caspase inhibitors, allows the extensive differentiation of human neuroblastoma cells.
- Author
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Yuste VJ, Sánchez-López I, Solé C, Encinas M, Bayascas JR, Boix J, and Comella JX
- Subjects
- Amino Acid Chloromethyl Ketones pharmacology, Caspase Inhibitors, Cell Differentiation drug effects, Humans, Neurites drug effects, Neurites pathology, Neurites physiology, Neuroblastoma physiopathology, Tumor Cells, Cultured, bcl-X Protein, Apoptosis drug effects, Enzyme Inhibitors pharmacology, Neuroblastoma pathology, Proto-Oncogene Proteins c-bcl-2 pharmacology, Staurosporine pharmacology
- Abstract
Staurosporine is one of the best apoptotic inducers in different cell types including neuroblastomas. In this study we have compared the efficiency and final outcome of three different anti-apoptotic strategies in staurosporine-treated SH-SY5Y human neuroblastoma cells. At staurosporine concentrations up to 500 nm, z-VAD.fmk a broad-spectrum, noncompetitive inhibitor of caspases, reduced apoptosis in SH-SY5Y cells. At higher concentrations, z-VAD.fmk continued to inhibit caspases and the apoptotic phenotype but not cell death which seems to result from oxidative damage. Stable over-expression of Bcl-2 in SH-SY5Y protected cells from death at doses of staurosporine up to 1 microm. At higher doses, cytochrome c release from mitochondria occurred, caspases were activated and cells died by apoptosis. Therefore, we conclude that Bcl-2 increased the threshold for apoptotic cell death commitment. Over-expression of Bcl-X(L) was far more effective than Bcl-2. Bcl-X(L) transfected cells showed a remarkable resistance staurosporine-induced cytochrome c release and associated apoptotic changes and survived for up to 15 days in 1 microm staurosporine. In these conditions, SH-SY5Y displayed a remarkable phenotype of neuronal differentiation as assessed by neurite outgrowth and expression of neurofilament, Tau and MAP-2 neuronal specific proteins.
- Published
- 2002
- Full Text
- View/download PDF
35. The absence of oligonucleosomal DNA fragmentation during apoptosis of IMR-5 neuroblastoma cells: disappearance of the caspase-activated DNase.
- Author
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Yuste VJ, Bayascas JR, Llecha N, Sánchez-López I, Boix J, and Comella JX
- Subjects
- Base Sequence, Chromatin metabolism, DNA Primers, DNA, Neoplasm metabolism, Humans, Hydrolysis, Tumor Cells, Cultured, Apoptosis, Deoxyribonucleases metabolism, Neuroblastoma pathology, Nucleosomes metabolism
- Abstract
Caspase-activated DNase is responsible for the oligonucleosomal DNA degradation during apoptosis. DNA degradation is thought to be important for multicellular organisms to prevent oncogenic transformation or as a mechanism of viral defense. It has been reported that certain cells, including some neuroblastoma cell lines such as IMR-5, enter apoptosis without digesting DNA in such a way. We have analyzed the causes for the absence of DNA laddering in staurosporine-treated IMR-5 cells, and we have found that most of the molecular mechanisms controlling apoptosis are well preserved in this cell line. These include degradation of substrates for caspases, blockade of cell death by antiapoptotic genes such as Bcl-2 or Bcl-X(L), or normal levels and adequate activation of caspase-3. Moreover, these cells display normal levels of caspase-activated DNase and its inhibitory protein, inhibitor of caspase-activated DNase, and their cDNA sequences are identical to those reported previously. Nevertheless, IMR-5 cells lose caspase-activated DNase during apoptosis and recover their ability to degrade DNA when human recombinant caspase-activated DNase is overexpressed. Our results lead to the conclusion that caspase-activated DNase is processed during apoptosis of IMR-5 cells, making these cells a good model to study the relevance of this endonuclease in physiological or pathological conditions.
- Published
- 2001
- Full Text
- View/download PDF
36. Receptors of the glial cell line-derived neurotrophic factor family of neurotrophic factors signal cell survival through the phosphatidylinositol 3-kinase pathway in spinal cord motoneurons.
- Author
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Soler RM, Dolcet X, Encinas M, Egea J, Bayascas JR, and Comella JX
- Subjects
- Animals, Cell Survival drug effects, Chick Embryo, Chromones pharmacology, Culture Media, Conditioned, Enzyme Inhibitors pharmacology, Flavonoids pharmacology, Glial Cell Line-Derived Neurotrophic Factor, Glial Cell Line-Derived Neurotrophic Factor Receptors, In Situ Hybridization, Mitogen-Activated Protein Kinases metabolism, Morpholines pharmacology, Motor Neurons cytology, Motor Neurons physiology, Muscles physiology, Phosphorylation, Proto-Oncogene Proteins drug effects, Proto-Oncogene Proteins genetics, Proto-Oncogene Proteins c-ret, Receptor Protein-Tyrosine Kinases drug effects, Receptor Protein-Tyrosine Kinases genetics, Reverse Transcriptase Polymerase Chain Reaction, Signal Transduction drug effects, Drosophila Proteins, Motor Neurons drug effects, Nerve Growth Factors pharmacology, Nerve Tissue Proteins pharmacology, Phosphatidylinositol 3-Kinases metabolism, Proto-Oncogene Proteins physiology, Receptor Protein-Tyrosine Kinases physiology, Signal Transduction physiology, Spinal Cord physiology
- Abstract
The members of the glial cell line-derived neurotrophic factor (GDNF) family of neurotrophic factors (GDNF, neurturin, persephin, and artemin) are able to promote in vivo and in vitro survival of different neuronal populations, including spinal cord motoneurons. These factors signal via multicomponent receptors that consist of the Ret receptor tyrosine kinase plus a member of the GDNF family receptor alpha (GRFalpha) family of glycosylphosphatidylinositol-linked coreceptors. Activation of the receptor induces Ret phosphorylation that leads the survival-promoting effects. Ret phosphorylation causes the activation of several intracellular pathways, but the biological effects caused by the activation of each of these pathways are still unknown. In the present work, we describe the ability of the GDNF family members to promote chicken motoneuron survival in culture. We show the presence of Ret and GFRalpha-1, GFRalpha-2, and GFRalpha-4 in chicken motoneurons using in situ hybridization and reverse transcription-PCR techniques. By Western blot analysis and kinase assays, we demonstrate the ability of these factors to induce the phosphatidylinositol 3 kinase (PI 3-kinase) and the extracellular regulated kinase (ERK)-mitogen-activated protein (MAP) kinase pathways activation. To characterize the involvement of these pathways in the survival effect, we used the PI 3-kinase inhibitor LY 294002 and the MAP kinase and ERK kinase (MEK) inhibitor PD 98059. We demonstrate that LY 294002, but not PD 98059, prevents GDNF-, neurturin-, and persephin-induced motoneuron survival, suggesting that PI 3-kinase intracellular pathway is responsible in mediating the neurotrophic effect.
- Published
- 1999
37. A homeobox gene of the orthodenticle family is involved in antero-posterior patterning of regenerating planarians.
- Author
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Stornaiuolo A, Bayascas JR, Salò E, and Boncinelli E
- Subjects
- Amino Acid Sequence, Animals, Drosophila Proteins, Head, Homeodomain Proteins chemistry, Molecular Sequence Data, Planarians physiology, Tail, Cell Differentiation genetics, Gene Expression, Genes, Homeobox, Homeodomain Proteins genetics, Planarians genetics, Regeneration
- Abstract
We studied the expression of DtOtx, a homeobox gene of the freshwater planarian Dugesia tigrina closely related to the Drosophila orthodenticle (otd) and vertebrate Otx genes, which are known to control head development in both fruit flies and vertebrates. DtOtx was not significantly expressed in adult planarians but it was activated within one hour in regenerating tissues with a clearly asymmetric pattern. Animals sectioned transversally, either between the head and the pharynx, or caudal to the pharynx, give rise to a head-containing fragment regenerating a tail region and to a tail-containing fragment regenerating a head region. DtOtx was found to be activated in both regeneration blastemas but its transcripts were much more abundant in the head-regenerating tissues than in the tail-regenerating tissues. The same asymmetric distribution of DtOtx transcripts was observed in central portions of the body regenerating both head and tail structures and in animals laterally regenerating after a longitudinal cut. These data suggest a role of this gene in patterning the body axis of these primitive bilateria, at least during regeneration.
- Published
- 1998
38. Platyhelminthes have a hox code differentially activated during regeneration, with genes closely related to those of spiralian protostomes.
- Author
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Bayascas JR, Castillo E, and Saló E
- Subjects
- Amino Acid Sequence, Animals, Base Sequence, DNA Primers, Gene Expression Regulation, Developmental, Phylogeny, Platyhelminths genetics, Reverse Transcriptase Polymerase Chain Reaction, Sequence Homology, Amino Acid, Genes, Homeobox, Platyhelminths physiology, Regeneration genetics
- Abstract
In recent years the characterization of Hox genes in different phyla has led to the suggestion of a universal role for these genes in animal axis determination. Some phyla, such as Platyhelminthes, have not yielded easily to such analysis, although a range of Hox genes have been shown to be present. In this report we present data concerning the relatively large number of Hox genes with a close similarity to representatives of annelids, supporting a phylogenetic clustering of Platyhelminthes within the spiralian protostomes. We also point out the permanent presence of Hox transcripts in adult planarians, with two classes distinguishable by their different patterns of axial expression: some are expressed uniformly, whilst a second group shows a nested expression with graded anterior expression boundaries. During posterior regeneration the nested Hox genes increase differentially depending on the level of sectioning, and then turn on gradually to recover the differential axial pattern of intact adults. These molecular results and others at the cellular level support the previous hypothesis that Platyhelminthes may have become simplified by progenesis.
- Published
- 1998
- Full Text
- View/download PDF
39. Characterization of platyhelminth POU domain genes: ubiquitous and specific anterior nerve cell expression of different epitopes of GtPOU-1.
- Author
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Muñoz-Mármol AM, Casali A, Miralles A, Bueno D, Bayascas JR, Romero R, and Saló E
- Subjects
- Amino Acid Sequence, Animals, Antibodies, Monoclonal, Base Sequence, Helminth Proteins immunology, Homeodomain Proteins immunology, Homeodomain Proteins metabolism, Molecular Sequence Data, Organ Specificity, Phylogeny, Planarians physiology, Protein Processing, Post-Translational, Recombinant Fusion Proteins genetics, Recombinant Fusion Proteins immunology, Recombinant Fusion Proteins metabolism, Regeneration, Reverse Transcriptase Polymerase Chain Reaction, Sequence Analysis, Sequence Homology, Amino Acid, Transcription Factors immunology, Transcription Factors metabolism, Transcription, Genetic, Helminth Proteins genetics, Helminth Proteins metabolism, Homeodomain Proteins genetics, Planarians genetics, Transcription Factors genetics
- Abstract
POU domain proteins are a large family of transcription factors that have been identified in a variety of metazoans, from freshwater sponges, planarians and nematodes to arthropods, echinoderms and vertebrates. Many of these proteins are implicated in the development and establishment of the nervous system. In this paper we describe the identification of the planarian genes GtPOU-1, GtPOU-3 and GtPOU-4, which belong to the subclasses III and IV of POU-domain genes. Their similarity with other members of the POU family is restricted to the POU and homeo domains, plus some peptide sequences scattered in the linker and flanking regions. As with other subclass III POU genes, GtPOU-1 is devoid of introns. Axial transcript distribution by RT-PCR and immunohistochemical assays, performed with a polyclonal antibody raised against the GtPOU-1 fusion protein, indicate that both the GtPOU-1 transcript and protein are continuously expressed along the antero-posterior axis. A monoclonal antibody raised against the same fusion protein indicates that a GtPOU-1-specific epitope, probably obtained by post-translational modification, is present in neural cells from both the central and peripheral nerve systems of the adult planarian's anterior third. Moreover, the GtPOU-1-specific epitope shows a dynamic expression pattern during regeneration, always marking the most anterior region of the planarian nervous system. Both the rapid and general GtPOU-1-specific epitope modification, during posterior regeneration, indicate that regeneration is a global process involving all planarian regions, including those that are far from the wound, by a combination of morphallactic and epimorphic mechanisms., (Copyright 1998 Elsevier Science Ireland Ltd. All Rights Reserved.)
- Published
- 1998
- Full Text
- View/download PDF
40. Planarian homeobox gene Dtprd-1 is expressed in specific gland cells and belongs to a new family within the paired-like class.
- Author
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Muñoz-Mármol AM, Bayascas JR, Castillo E, Casali A, and Saló E
- Abstract
The genome of the planarian (Platyhelminthes; Turbellaria; Tricladida) Dugesia (Girardia) tigrina includes a paired-like type of homeobox gene. The Dtprd-1 gene encodes a protein of 382 amino acids. The open reading frame of Dtprd-1 is interrupted by two short introns of 65 and 56 bp, and one long intron of 4.8 kb. The intron positions are not located in the homeobox and are not shared with any other known paired-like gene. The Dtprd-1 homeodomain conserves most of the residues characteristic of the paired-like class. Similarity with other members of this class is low, except with the rat, mouse and Caenorhabditis elegans proteins PHD1, Uncx-4.1 and unc-4, with 86-83% of similarity in the homeodomain, plus several peptides in the flanking regions. Such proteins share specific residues in their homeodomains that can be used to define a new family in the paired-like class of genes. The spatial distribution of gene transcript and product in adult tissues, as revealed by RT-PCR, northern blots and polyclonal antibody, demonstrates that Dtprd-1 is highly expressed in cyanophilic gland cells located in the ventral parenchyma close to the nervous system. No expression is observed during the early stages of regeneration (0-3 days). This suggests a possible role for this homeobox gene within these secretory gland cells, but not in the pattern formation mechanisms known to occur at the early stages of regeneration.
- Published
- 1997
- Full Text
- View/download PDF
41. Planarian Hox genes: novel patterns of expression during regeneration.
- Author
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Bayascas JR, Castillo E, Muñoz-Mármol AM, and Saló E
- Subjects
- Amino Acid Sequence, Animals, Base Sequence, DNA Primers, Drosophila, Genomic Library, Homeodomain Proteins genetics, In Situ Hybridization, Leeches, Molecular Sequence Data, Multigene Family, Planarians genetics, Planarians growth & development, Polymerase Chain Reaction, Regeneration, Sequence Homology, Amino Acid, Gene Expression Regulation, Developmental, Genes, Helminth, Genes, Homeobox, Homeodomain Proteins biosynthesis, Homeodomain Proteins chemistry, Planarians physiology
- Abstract
Platyhelminthes are widely considered to be the sister group of coelomates (Philippe, H., Chenuil, A. and Adoutte, A. (1994)Development 1994 Supplement, 15-24) and the first organisms to show bilateral symmetry and cephalization. Within this phylum, the freshwater planarians (Turbellaria, Tricladida) have been used as model systems for studying bidirectional regeneration (Slack, J. M. W. (1980) J. Theor. Biol 82, 105-140). We have been attempting to identify potential pattern-control genes involved in the regeneration of planarian heads and tails after amputation. Since Hox cluster genes determine positional identity along the anteroposterior axis in a wide range of animals (Slack, J. M. W., Holland, P. W. H. and Graham, C. F. (1993) Nature 361,490-492), we performed an extensive search for Hox-related genes in the planarian Dugesia(G)tigrina. Sequence analyses of seven planarian Dthox genes (Dthox-A to Dthox-G) reveal high similarities with the homeodomain region of the Hox cluster genes, allowing us to assign planarian Dthox genes to anterior and medial Hox cluster paralogous groups. Whole-mount in situ hybridization studies in regenerating adults showed very early, synchronous and colocalized activation of Dthox-D, Dthox-A, Dthox-C, Dthox-E, Dthox-G and Dthox-F. After one hour of regeneration a clear expression was observed in all Dthox genes studied. In addition, all seemed to be expressed in the same regenerative tissue, although in the last stages of regeneration (9 to 15 days) a differential timing of deactivation was observed. The same Dthox genes were also expressed synchronously and were colocalized during intercalary regeneration, although their expression was delayed. Terminal regeneration showed identical Dthox gene expression in anterior and posterior blastemas, which may prevent these genes from directing the distinction between head and tail. Finally, continuous expression along the whole lateral blastema in sagittal regenerates reflected a ubiquitous Dthox response in all types of regeneration that was not related specifically with the anteroposterior polarity.
- Published
- 1997
- Full Text
- View/download PDF
42. Hox genes disobey colinearity and do not distinguish head from tail during planarian regeneration.
- Author
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Bayascas JR, Castillo E, Muñoz-Màrmol A, and Saló E
- Subjects
- Animals, Caenorhabditis elegans genetics, Drosophila genetics, Genes, Helminth, Genes, Insect, In Situ Hybridization, Multigene Family, Vertebrates genetics, Genes, Homeobox, Planarians genetics, Planarians physiology, Regeneration genetics
- Published
- 1996
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