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136 results on '"Baumstark, Manfred W."'

18. Analysis of the nitric oxide-cyclic guanosine monophosphate pathway in experimental liver cirrhosis suggests phosphodiesterase-5 as potential target to treat portal hypertension

Author

Schaffner, Denise, primary, Lazaro, Adhara, additional, Deibert, Peter, additional, Hasselblatt, Peter, additional, Stoll, Patrick, additional, Fauth, Lisa, additional, Baumstark, Manfred W, additional, Merfort, Irmgard, additional, Schmitt-Graeff, Annette, additional, and Kreisel, Wolfgang, additional

Published
2018
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25. Variability and reproducibility of carotid structural and functional parameters assessed with transcutaneous ultrasound – Results from the SAPALDIA Cohort Study

Author

Caviezel, Seraina, primary, Dratva, Julia, additional, Schaffner, Emmanuel, additional, Teynor, Alexandra, additional, Baumstark, Manfred W., additional, Schindler, Christian, additional, de Groot, Eric, additional, Burdet, Luc, additional, Rothe, Thomas, additional, Pons, Marco, additional, Gaspoz, Jean-Michel, additional, Rochat, Thierry, additional, Künzli, Nino, additional, Probst-Hensch, Nicole, additional, and Schmidt-Trucksäss, Arno, additional

Published
2013
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26. Crystallization of human low density lipoprotein (LDL), a large lipid-protein complex : collection of X-ray data at very low resolution

Author

Ritter, Stephan, Frey, Ingrid, Berg, Aloys, Keul, Joseph, Baumstark, Manfred W., and Diederichs, Kay

Subjects
ddc:570, Crystal, Data collection, Low density lipoprotein, Atherosclerosis
Abstract

Human LDL subfractions LDL-2 (d=1.031-1.034 g/ml) and LDL-5 (d=1.040-1.044 g/ml) were crystallized in different crystal forms using polyethylene glycol as a precipitant. Both fractions were from one donor. Crystals of LDL-5 were yellow, hexagonal, and showed no dichroism. Of LDL-2 two dichroitic crystal forms were obtained. One had a rod-like shape with deep notches at both ends (form A), the other was a more compact form with plain surfaces (form B). To be able to measure low order reflections down to 300 Å a special experimental setup was developed. One single crystal was used to obtain a complete native data set of LDL-2 (form A) with an overall internal R-factor of 4.5% for reflections from 100 to 28 Å. Data were collected under cryogenic conditions using synchrotron radiation. The space group is most probably C2 with unit cell dimensions of a=183 Å, b=421 Å, c=385 Å, α=γ=90°, β≈90°. Further optimization of the crystallization conditions and the search for heavy metal derivatives are in progress.

Published
1999

27. Frequency of secondary dyslipidemia in obese children

Author

Korsten-Reck,Ulrike, Kromeyer-Hauschild,Katrin, Korsten,Katrin, Baumstark,Manfred W, Dickhuth,Hans-H, Berg,Aloys, Korsten-Reck,Ulrike, Kromeyer-Hauschild,Katrin, Korsten,Katrin, Baumstark,Manfred W, Dickhuth,Hans-H, and Berg,Aloys

Abstract

Ulrike Korsten-Reck1, Katrin Kromeyer-Hauschild2, Katrin Korsten1, Manfred W Baumstark1, Hans-H Dickhuth1, Aloys Berg11Department of Rehabilitative and Preventive Sports Medicine, University Medical Center, University of Freiburg, 79106 Freiburg, Germany; 2Institute of Human Genetics and Anthropology, Friedrich-Schiller-University Jena, 07740 Jena, GermanyObjective: This paper reports the frequency, type, and degree of dyslipidemia in obese children before therapeutic intervention. The relationships between lipid values and weight status, as well as lipid values and physical fitness, of these children were also investigated.Design and methods: The initial examination of the Freiburg Intervention Trial for Obese Children (FITOC) measured the values of triglycerides (TG), total cholesterol (C), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C) in 546 obese children aged 7–12 (body mass index [BMI] > 97th percentile), and compared these values with those of the age- and sex-specific reference group in the Lipid Research Clinics Population Studies Data Book (LRC). Four groups were selected according to the following scheme: A, Normolipidemia; B, Hyper-LDL-cholesterolemia alone; C, Hypo-HDL-C + hypertriglyceridemia; D, Combined hyperlipidemia = Hyper-LDL-C + hypertriglyceridemia. Body mass index, BMI-SDS (corrected BMI), and physical performance in watt/kg body weight were measured.Results: A total of 45.8% of the overweight children showed an abnormal lipid profile. Ten percent of the children had high LDL-C levels (group B), while 15% had increased LDL-C and increased TG (group D) (higher prevalence in boys). In 18.9% we found increased TG, combined with decreased HDL-C values (group C).Conclusion: Obese children are at risk of dyslipoproteinemia and related diseases. Children with the highest BMI-SDS and lowest physical fitness have the lowest HDL-C values and increased TG, indicating a higher risk

Published
2008

29. Crystallization and Preliminary X-Ray Diffraction Data of Two Different Human Low-Density Lipoprotein (LDL) Subfractions

Author

Ritter, Stephan, Frey, Ingrid, Grathwohl, Dominik, Keul, Joseph, Baumstark, Manfred W., and Diederichs, Kay

Subjects
crystallization, ddc:570, lipids (amino acids, peptides, and proteins), human low-density lipoprotein subfractions, X-ray diffraction
Abstract

Human LDL subfractions LDL-2 (d = 1.031 1.034 g/ml) and LDL-5 (d = 1.040 1.044 g/ml) were crystallized in two different crystal forms by using polyethylene glycol as a precipitant. Both fractions were from one donor. Crystals of LDL-5 were yellow, hexagonal, and showed no dichroism. Crystals of LDL-2 were of the same color, had a rodlike shape with notches at both ends, and were highly dichroitic. LDL-2 crystals diffracted to a resolution of 29 Å by using synchrotron radiation. Indexing in P1 resulted in preliminary parameters for the reduced cell of a = 171 Å, b = 438 Å, c = 519 Å, α = 102°, β = 99°, γ = 91. These dimensions are consistent with the size of LDL particles. Using Fourier transform infrared spectroscopy (FTIR) and agarose gel electrophoresis, we could further confirm that the crystals consist of LDL. The FTIR spectrum showed bands characteristic for lipids and protein. Dissolved crystals exhibited a mobility similar to native LDL in agarose gels and could be stained with anti-human apolipoprotein B (apoB).

Published
1997

43. Homozygous Familial Defective Apolipoprotein B-100

Author

Schaefer, Juergen R., primary, Scharnagl, Hubert, additional, Baumstark, Manfred W., additional, Schweer, Horst, additional, Zech, Loren A., additional, Seyberth, Hansjörg, additional, Winkler, Karl, additional, Steinmetz, Armin, additional, and März, Winfried, additional

Published
1997
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49. Low-resolution structure determination of Na+-translocating NADH:ubiquinone oxidoreductase from Vibrio cholerae by ab initio phasing and electron microscopy.

Author

Lunin, Vladimir Y., Lunina, Natalia L., Casutt, Marco S., Knoops, Kèvin, Schaffitzel, Christiane, Steuber, Julia, Fritz, Günter, and Baumstark, Manfred W.

Subjects
MOLECULAR structure, ELECTRON microscopes, OXIDOREDUCTASES, NADH dehydrogenase, VIBRIO cholerae, ANTIBIOTICS, ELECTRON distribution
Abstract

A low-resolution structure of the Na+-translocating NADH:ubiquinone oxidoreductase from the human pathogen Vibrio cholerae was determined by ab initio phasing and independently confirmed by electron microscopy. This multi-subunit membrane-protein complex (molecular weight 210 kDa) generates an Na+ gradient that is essential for substrate uptake, motility, pathogenicity and efflux of antibiotics. The obtained 16 Å resolution electron density-map revealed an asymmetric particle with a central region of low electron density and a putative detergent region, and allowed the identification of the transmembrane regions of the complex. [ABSTRACT FROM AUTHOR]

Published
2012
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50. A soy-based supplement alters energy metabolism but not the exercise-induced stress response.

Author

Berg, Aloys, Schaffner, Denise, Pohlmann, Yolanda, Baumstark, Manfred W., Deibert, Peter, König, Daniel, and Gollhofer, Albert

Subjects
PHYSICAL fitness, SOY proteins, RANDOMIZED controlled trials, PHYSICAL training & conditioning, DIETARY supplements, HUMAN body composition, METABOLISM
Abstract

Objective To determine the changes in endurance capacity as well as in metabolic, hormonal and inflammatory markers induced by endurance training combined with a soy protein based supplement. Design Randomized controlled study consisting of moderate endurance training without (G0) or with (G1) a soy protein based supplement. Subjects Two groups of 15 subjects (10 males and 5 females in each group): healthy sports students aged 23.6 ± 1.9 years. Measurements Body composition (body mass (BM), body density (BD) by air displacement) and physical fitness (determined by treadmill ergometry) were measured at baseline and after 6 weeks of the intervention; changes in circulating metabolic and hormonal parameters (glucose, lactate, urea, uric acid, ammonia, cortisol, insulin, IGF-1), and exercise-induced stress and inflammatory markers (CK, LDH, myoglobin, hs-CRP, IL-6, IL-10, blood cell counts) were determined after the intervention period in a field test (11.5 km running on hilly ground). Results 30 participants completed the 6-week study; 28 students were able to perform the field test. No significant changes in BM and BD were noted after intervention with only slight increases in running performance and maximum aerobic capacity in the total group (2%, p=0.016). Subjects in the G1 group showed significant improvements in running velocity and lower lactate values following the intervention (-12%, p=0,003). In addition, the G1 group showed significantly lower differences in the exercise-induced increase of metabolic parameters (triglycerides, uric acid) and insulin in the post-exercise recovery period. Conclusions Our data suggest that moderate endurance training in combination with a soy-based protein supplement improves aerobic energy supply and metabolic function in healthy sports students, even without changes in body composition and without changes in the exercise-induced stress and inflammatory reaction. [ABSTRACT FROM AUTHOR]

Published
2012

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