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2. Supplementary Data from Glypican-3–Specific CAR T Cells Coexpressing IL15 and IL21 Have Superior Expansion and Antitumor Activity against Hepatocellular Carcinoma

Author

Batra, Sai Arun, primary, Rathi, Purva, primary, Guo, Linjie, primary, Courtney, Amy N., primary, Fleurence, Julien, primary, Balzeau, Julien, primary, Shaik, Rahamthulla S., primary, Nguyen, Thao P., primary, Wu, Meng-Fen, primary, Bulsara, Shaun, primary, Mamonkin, Maksim, primary, Metelitsa, Leonid S., primary, and Heczey, Andras, primary

Published
2023
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3. Data from Glypican-3–Specific CAR T Cells Coexpressing IL15 and IL21 Have Superior Expansion and Antitumor Activity against Hepatocellular Carcinoma

Author

Batra, Sai Arun, primary, Rathi, Purva, primary, Guo, Linjie, primary, Courtney, Amy N., primary, Fleurence, Julien, primary, Balzeau, Julien, primary, Shaik, Rahamthulla S., primary, Nguyen, Thao P., primary, Wu, Meng-Fen, primary, Bulsara, Shaun, primary, Mamonkin, Maksim, primary, Metelitsa, Leonid S., primary, and Heczey, Andras, primary

Published
2023
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4. Glypican-3–Specific CAR T Cells Coexpressing IL15 and IL21 Have Superior Expansion and Antitumor Activity against Hepatocellular Carcinoma

Author

Batra, Sai Arun, primary, Rathi, Purva, additional, Guo, Linjie, additional, Courtney, Amy N., additional, Fleurence, Julien, additional, Balzeau, Julien, additional, Shaik, Rahamthulla S., additional, Nguyen, Thao P., additional, Wu, Meng-Fen, additional, Bulsara, Shaun, additional, Mamonkin, Maksim, additional, Metelitsa, Leonid S., additional, and Heczey, Andras, additional

Published
2020
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12. PCR ASSAY DETECTSMANNHEIMIA HAEMOLYTICAIN CULTURE-NEGATIVE PNEUMONIC LUNG TISSUES OF BIGHORN SHEEP (OVIS CANADENSIS) FROM OUTBREAKS IN THE WESTERN USA, 2009–2010

Author

Shanthalingam, Sudarvili, primary, Goldy, Andrea, additional, Bavananthasivam, Jegarubee, additional, Subramaniam, Renuka, additional, Batra, Sai Arun, additional, Kugadas, Abirami, additional, Raghavan, Bindu, additional, Dassanayake, Rohana P., additional, Jennings-Gaines, Jessica E., additional, Killion, Halcyon J., additional, Edwards, William H., additional, Ramsey, Jennifer M., additional, Anderson, Neil J., additional, Wolff, Peregrine L., additional, Mansfield, Kristin, additional, Bruning, Darren, additional, and Srikumaran, Subramaniam, additional

Published
2014
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13. Acylation Enhances, but Is Not Required for, the Cytotoxic Activity of Mannheimia haemolyticaLeukotoxin in Bighorn Sheep

Author

Batra, Sai A., Shanthalingam, Sudarvili, Munske, Gerhard R., Raghavan, Bindu, Kugadas, Abirami, Bavanthasivam, Jegarubee, Highlander, Sarah K., and Srikumaran, Subramaniam

Abstract

ABSTRACTMannheimia haemolyticacauses pneumonia in domestic and wild ruminants. Leukotoxin (Lkt) is the most important virulence factor of the bacterium. It is encoded within the four-gene lktCABDoperon: lktA encodes the structural protoxin, and lktCencodes a trans-acylase that adds fatty acid chains to internal lysine residues in the protoxin, which is then secreted from the cell by a type 1 secretion system apparatus encoded by lktBand lktD. It has been reported that LktC-mediated acylation is necessary for the biological effects of the toxin. However, an LktC mutant that we developed previously was only partially attenuated in its virulence for cattle. The objective of this study was to elucidate the role of LktC-mediated acylation in Lkt-induced cytotoxicity. We performed this study in bighorn sheep (Ovis canadensis) (BHS), since they are highly susceptible to M. haemolyticainfection. The LktC mutant caused fatal pneumonia in 40% of inoculated BHS. On necropsy, a large number of necrotic polymorphonuclear leukocytes (PMNs) were observed in the lungs. Lkt from the mutant was cytotoxic to BHS PMNs in an in vitrocytotoxicity assay. Flow cytometric analysis of mutant Lkt-treated PMNs revealed the induction of necrosis. Scanning electron microscopic analysis revealed the presence of pores and blebs on mutant-Lkt-treated PMNs. Mass spectrometric analysis confirmed that the mutant secreted an unacylated Lkt. Taken together, these results suggest that acylation is not necessary for the cytotoxic activity of M. haemolyticaLkt but that it enhances the potency of the toxin.

Published
2015
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14. PCR ASSAY DETECTS MANNHEIMIA HAEMOLYTICA IN CULTURE-NEGATIVE PNEUMONIC LUNG TISSUES OF BIGHORN SHEEP (OVIS CANADENSIS) FROM OUTBREAKS IN THE WESTERN USA, 2009-2010.

Author

Shanthalingam, Sudarvili, Goldy, Andrea, Bavananthasivam, Jegarubee, Subramaniam, Renuka, Batra, Sai Arun, Kugadas, Abirami, Raghavan, Bindu, Dassanayake, Rohana P., Jennings-Gaines, Jessica E., Killion, Halcyon J., Edwards, William H., Ramsey, Jennifer M., Anderson, Neil J., Wolff, Peregrine L., Mansfield, Kristin, Bruning, Darren, and Srikumaran, Subramaniam

Abstract

The article reports on the development a polymerase chain reaction (PCR) assay specific for Mannheimia haemolytica (M.h.) to determine its presence in the pneumonic lungs of bighorn sheep during the 2009-10 outbreaks in three states. M. h. was detected in 77% of archived pneumonic lung tissues that were negative by culture. Collective results show that culture-based methods are unreliable for detecting M. h., and that leukotoxin-positive M.h. was a predominant agent in the outbreaks.

Published
2014
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15. A sensitive & specific multiplex PCR assay for simultaneous detection of Bacillus anthracis, Yersinia pestis, Burkholderia pseudomallei & Brucella species.

Author

Arun Batra, Sai, Krupanidhi, S., and Tuteja, Urmil

Subjects
*POLYMERASE chain reaction, *BACILLUS anthracis, *YERSINIA pestis, *BURKHOLDERIA pseudomallei, *BRUCELLA, *BIOLOGICAL weapons, *PATHOGENIC microorganisms
Abstract

Background & objectives: Bacillus anthracis, Yersinia pestis, Burkholderja pseudoinallei and Brucella species are potential biowarfare agents. Classical bacteriological methods for their identification are cumbersome, time consuming and of potential risk to the handler. Methods: We describe a sensitive and specific multiplex polymerase chain reaction (mPCR) assay involving novel primers sets for the simultaneous detection of B. anthracis, Y pestis, B. pseudomallei and Brucella species. An additional non-competitive internal amplification control (IAC) was also included. Results: The mPCR was found to be specific when tested against closely related organisms. The sensitivity of the assay in spiked blood samples was 50 colony forming units (cfus)/25 μl reaction, for the detection of B. anthracis, Y pestis and Brucella species; and 150 cfus/25 μl reaction, for B. pseudornallei. The assay proved useful in correctly and promptly identifing the clinical isolates of the targeted agents recovered from patients, compared to the gold standard culture methods. Interpretation & conclusion: The assay described in this study showed promise to be useful in application as a routine detection cum diagnostic method for these pathogens. [ABSTRACT FROM AUTHOR]

Published
2013

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