25 results on '"Batista LC"'
Search Results
2. ESTABLISHMENT OF A CRISPR/CAS9-BASED GENOME EDITING PLATFORM FOR THE GENERATION OF AN OFF-THE-SHELF ALLOGENEIC CAR-T CELL MODEL
- Author
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Lima, SCG, primary, Brand, H, additional, Batista, LC, additional, Fantacini, DMC, additional, Coelho, MGB, additional, Castro, FA, additional, Covas, DT, additional, and Souza, LEB, additional
- Published
- 2021
- Full Text
- View/download PDF
3. POLARIZAÇÃO DE LINFÓCITOS T AO FENÓTIPO T HELPER/CITOTÓXICO 17 PARA USO EM TERAPIAS CELULARES AVANÇADAS
- Author
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Brand, H, primary, Batista, LC, additional, Fantacini, DMC, additional, Lima, SCG, additional, Covas, DT, additional, and Souza, LEB, additional
- Published
- 2021
- Full Text
- View/download PDF
4. ANTI-CD19 CAR T CELL TRACKING PLATFORM FOR PRECLINICAL AND CLINICAL STUDIES SHOWS GREAT TUMOR BURDEN REDUCTION LINKED TO STEADY PERSISTENCE
- Author
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Batista, LC, primary, Brand, H, additional, Fantacini, DMC, additional, Covas, DT, additional, and Souza, LEB, additional
- Published
- 2021
- Full Text
- View/download PDF
5. DEVELOPMENT OF CRISPR/CAS9-BASED GENOME EDITING PLATFORM FOR THE GENERATION OF ALLOGENEIC CAR-T CELLS
- Author
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Lima, SCG, primary, Brand, H, additional, Batista, LC, additional, Fantacini, DMC, additional, Coelho, MGB, additional, Castro, FA, additional, Covas, DT, additional, and Souza, LEB, additional
- Published
- 2021
- Full Text
- View/download PDF
6. EVALUATION OF ANTI-CD19 CAR-T CELL PERSISTENCE AND EFFICACY USING A DEVELOPED MULTIPARAMETRIC FLOW CYTOMETRY AND QPCR TRACKING PLATFORM FOR PRECLINICAL AND CLINICAL STUDIES
- Author
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Batista, LC, primary, Brand, H, additional, Fantacini, DMC, additional, Covas, DT, additional, and Souza, LEB, additional
- Published
- 2021
- Full Text
- View/download PDF
7. Cornelia de Lange syndrome in diverse populations.
- Author
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Dowsett, L, Porras, AR, Kruszka, P, Davis, B, Hu, T, Honey, E, Badoe, E, Thong, M-K, Leon, E, Girisha, KM, Shukla, A, Nayak, SS, Shotelersuk, V, Megarbane, A, Phadke, S, Sirisena, ND, Dissanayake, VHW, Ferreira, CR, Kisling, MS, Tanpaiboon, P, Uwineza, A, Mutesa, L, Tekendo-Ngongang, C, Wonkam, A, Fieggen, K, Batista, LC, Moretti-Ferreira, D, Stevenson, RE, Prijoles, EJ, Everman, D, Clarkson, K, Worthington, J, Kimonis, V, Hisama, F, Crowe, C, Wong, P, Johnson, K, Clark, RD, Bird, L, Masser-Frye, D, McDonald, M, Willems, P, Roeder, E, Saitta, S, Anyane-Yeoba, K, Demmer, L, Hamajima, N, Stark, Z, Gillies, G, Hudgins, L, Dave, U, Shalev, S, Siu, V, Ades, A, Dubbs, H, Raible, S, Kaur, M, Salzano, E, Jackson, L, Deardorff, M, Kline, A, Summar, M, Muenke, M, Linguraru, MG, Krantz, ID, Dowsett, L, Porras, AR, Kruszka, P, Davis, B, Hu, T, Honey, E, Badoe, E, Thong, M-K, Leon, E, Girisha, KM, Shukla, A, Nayak, SS, Shotelersuk, V, Megarbane, A, Phadke, S, Sirisena, ND, Dissanayake, VHW, Ferreira, CR, Kisling, MS, Tanpaiboon, P, Uwineza, A, Mutesa, L, Tekendo-Ngongang, C, Wonkam, A, Fieggen, K, Batista, LC, Moretti-Ferreira, D, Stevenson, RE, Prijoles, EJ, Everman, D, Clarkson, K, Worthington, J, Kimonis, V, Hisama, F, Crowe, C, Wong, P, Johnson, K, Clark, RD, Bird, L, Masser-Frye, D, McDonald, M, Willems, P, Roeder, E, Saitta, S, Anyane-Yeoba, K, Demmer, L, Hamajima, N, Stark, Z, Gillies, G, Hudgins, L, Dave, U, Shalev, S, Siu, V, Ades, A, Dubbs, H, Raible, S, Kaur, M, Salzano, E, Jackson, L, Deardorff, M, Kline, A, Summar, M, Muenke, M, Linguraru, MG, and Krantz, ID
- Abstract
Cornelia de Lange syndrome (CdLS) is a dominant multisystemic malformation syndrome due to mutations in five genes-NIPBL, SMC1A, HDAC8, SMC3, and RAD21. The characteristic facial dysmorphisms include microcephaly, arched eyebrows, synophrys, short nose with depressed bridge and anteverted nares, long philtrum, thin lips, micrognathia, and hypertrichosis. Most affected individuals have intellectual disability, growth deficiency, and upper limb anomalies. This study looked at individuals from diverse populations with both clinical and molecularly confirmed diagnoses of CdLS by facial analysis technology. Clinical data and images from 246 individuals with CdLS were obtained from 15 countries. This cohort included 49% female patients and ages ranged from infancy to 37 years. Individuals were grouped into ancestry categories of African descent, Asian, Latin American, Middle Eastern, and Caucasian. Across these populations, 14 features showed a statistically significant difference. The most common facial features found in all ancestry groups included synophrys, short nose with anteverted nares, and a long philtrum with thin vermillion of the upper lip. Using facial analysis technology we compared 246 individuals with CdLS to 246 gender/age matched controls and found that sensitivity was equal or greater than 95% for all groups. Specificity was equal or greater than 91%. In conclusion, we present consistent clinical findings from global populations with CdLS while demonstrating how facial analysis technology can be a tool to support accurate diagnoses in the clinical setting. This work, along with prior studies in this arena, will assist in earlier detection, recognition, and treatment of CdLS worldwide.
- Published
- 2019
8. Foreign Body Ingestion in Adults: Clinical Characteristics and Management
- Author
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Diaz-Gomez, M, primary, Martinez-Batista, LC, additional, and Carreiro-Rodriguez, M, additional
- Published
- 2016
- Full Text
- View/download PDF
9. Brazilian growth charts for Williams-Beuren Syndrome at ages 2 to 18 years.
- Author
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Strafacci ASL, Bertapelli F, Kim CA, Rivadeneira MJ, Honjo RS, Domenici Kulikowski L, Ferreira DM, Batista LC, Lopes VLGDS, and Guerra Junior G
- Subjects
- Humans, Male, Adolescent, Female, Child, Preschool, Brazil epidemiology, Child, Retrospective Studies, Longitudinal Studies, Reference Values, Sex Factors, Age Factors, Williams Syndrome diagnosis, Growth Charts, Body Height physiology, Body Mass Index, Body Weight
- Abstract
Objective: To develop growth charts for weight-for-age, height-for-age, and body mass index (BMI)-for-age for both genders aged 2 to 18 years for Brazilian patients with Williams-Beuren Syndrome (WBS)., Methods: This is a multicenter, retrospective, and longitudinal study, data were collected from the medical records of boys and girls with a confirmed diagnosis of WBS in three large university centers in the state of Sao Paulo, Brazil. Growth charts stratified by gender and age in years were developed using LMSchartmaker Pro software. The LMS (Lambda Mu Sigma) method was used to model the charts . The quality of the settings was checked by worm plots., Results: The first Brazilian growth charts for weight-for-age, height-for-age, and BMI-for-age stratified by gender were constructed for WBS patients aged 2 to 18 years., Conclusion: The growth charts developed in this study can help to guide family members and to improve the health care offered by health professionals., Competing Interests: Conflicts of interest The authors declare no conflicts of interest., (Copyright © 2023. Published by Elsevier Editora Ltda.)
- Published
- 2024
- Full Text
- View/download PDF
10. Characteristics of music intervention to reduce anxiety in patients undergoing cardiac catheterization: scoping review.
- Author
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Batista LC, Melo MN, Cruz DALMD, and Gengo E Silva Butcher RC
- Abstract
The characteristics of music interventions for reducing anxiety in patients undergoing cardiac catheterization were mapped. A scoping review was conducted according to the Joanna Briggs Institute methodology. Searches were performed in electronic portals and databases PubMed, CINAHL, PsycINFO, Cochrane, EMBASE, Scopus, LILACS, CAPES Thesis Portal (Brazil), DART-Europe E-theses Portal, Theses Canada Portal, Pro-Quest, and Google Scholar databases, gray literature, with no limitation on the year of publication. Eighteen articles were included in the search. The characteristics of the interventions were heterogeneous and not comprehensively described in the primary studies. The songs were predominantly of a single genre, instrumental, and selected by the interventionist, with a rhythm between 60 and 80 beats per minute. The interventions were delivered in a single session, mostly in the catheterization laboratory, before or during the procedure, by means of digital audio and earphones for over 20 min. The heterogeneity of interventions and incompleteness of information in the studies compromises the advancement of knowledge on the effects of music on health outcomes., Competing Interests: The authors declare no conflict of interest., (© 2022 The Author(s).)
- Published
- 2022
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11. Strategies to Enhance the Therapeutic Efficacy, Applicability, and Safety of Genetically Engineered Immune Cells.
- Author
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de Lima SCG, Fantacini DMC, Batista LC, Silveira RM, Furtado IP, Rossetti R, Brand H, Covas DT, and de Souza LEB
- Subjects
- Cell- and Tissue-Based Therapy, Humans, Immunotherapy, Adoptive, Receptors, Antigen, T-Cell genetics, T-Lymphocytes, Neoplasms therapy, Receptors, Chimeric Antigen
- Abstract
The field of cell therapy is leading a paradigm shift in drug development. The recent convergence of several fields, including immunology, genetics, and synthetic biology, now allows for the introduction of artificial receptors and the design of entire genetic circuitries to finely program the behavior of injected cells. A prime example of these next-generation living drugs comes in the form of T cells expressing chimeric antigen receptors (CARs), which have already demonstrated definitive evidence of therapeutic efficacy against some hematological malignancies. However, several obstacles still restrict the antitumor efficacy of and impair the widespread use of CAR-T cells. Critical challenges include limited persistence and antitumor activity in vivo, antigen escape, scarcity of suitable single markers for targeting, and therapy-related toxicity. Nevertheless, intense research activity in this field has resulted in a plethora of creative solutions to address each of these limitations. In this review, we provide a comprehensive snapshot of the current strategies used to enhance the therapeutic efficacy, applicability, and safety of genetically engineered immune cells to treat cancer.
- Published
- 2021
- Full Text
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12. Rubinstein-Taybi syndrome in diverse populations.
- Author
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Tekendo-Ngongang C, Owosela B, Fleischer N, Addissie YA, Malonga B, Badoe E, Gupta N, Moresco A, Huckstadt V, Ashaat EA, Hussen DF, Luk HM, Lo IFM, Hon-Yin Chung B, Fung JLF, Moretti-Ferreira D, Batista LC, Lotz-Esquivel S, Saborio-Rocafort M, Badilla-Porras R, Penon Portmann M, Jones KL, Abdul-Rahman OA, Uwineza A, Prijoles EJ, Ifeorah IK, Llamos Paneque A, Sirisena ND, Dowsett L, Lee S, Cappuccio G, Kitchin CS, Diaz-Kuan A, Thong MK, Obregon MG, Mutesa L, Dissanayake VHW, El Ruby MO, Brunetti-Pierri N, Ekure EN, Stevenson RE, Muenke M, and Kruszka P
- Subjects
- Adolescent, Adult, Case-Control Studies, Child, Child, Preschool, Cohort Studies, Female, Genetic Association Studies, Humans, Infant, International Agencies, Male, Middle Aged, Prognosis, Rubinstein-Taybi Syndrome genetics, Rubinstein-Taybi Syndrome pathology, Young Adult, E1A-Associated p300 Protein genetics, Ethnicity genetics, Face abnormalities, Genetics, Population, Mutation, Rubinstein-Taybi Syndrome epidemiology
- Abstract
Rubinstein-Taybi syndrome (RSTS) is an autosomal dominant disorder, caused by loss-of-function variants in CREBBP or EP300. Affected individuals present with distinctive craniofacial features, broad thumbs and/or halluces, and intellectual disability. RSTS phenotype has been well characterized in individuals of European descent but not in other populations. In this study, individuals from diverse populations with RSTS were assessed by clinical examination and facial analysis technology. Clinical data of 38 individuals from 14 different countries were analyzed. The median age was 7 years (age range: 7 months to 47 years), and 63% were females. The most common phenotypic features in all population groups included broad thumbs and/or halluces in 97%, convex nasal ridge in 94%, and arched eyebrows in 92%. Face images of 87 individuals with RSTS (age range: 2 months to 47 years) were collected for evaluation using facial analysis technology. We compared images from 82 individuals with RSTS against 82 age- and sex-matched controls and obtained an area under the receiver operating characteristic curve (AUC) of 0.99 (p < .001), demonstrating excellent discrimination efficacy. The discrimination was, however, poor in the African group (AUC: 0.79; p = .145). Individuals with EP300 variants were more effectively discriminated (AUC: 0.95) compared with those with CREBBP variants (AUC: 0.93). This study shows that clinical examination combined with facial analysis technology may enable earlier and improved diagnosis of RSTS in diverse populations., (© 2020 Wiley Periodicals LLC.)
- Published
- 2020
- Full Text
- View/download PDF
13. Cornelia de Lange syndrome in diverse populations.
- Author
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Dowsett L, Porras AR, Kruszka P, Davis B, Hu T, Honey E, Badoe E, Thong MK, Leon E, Girisha KM, Shukla A, Nayak SS, Shotelersuk V, Megarbane A, Phadke S, Sirisena ND, Dissanayake VHW, Ferreira CR, Kisling MS, Tanpaiboon P, Uwineza A, Mutesa L, Tekendo-Ngongang C, Wonkam A, Fieggen K, Batista LC, Moretti-Ferreira D, Stevenson RE, Prijoles EJ, Everman D, Clarkson K, Worthington J, Kimonis V, Hisama F, Crowe C, Wong P, Johnson K, Clark RD, Bird L, Masser-Frye D, McDonald M, Willems P, Roeder E, Saitta S, Anyane-Yeoba K, Demmer L, Hamajima N, Stark Z, Gillies G, Hudgins L, Dave U, Shalev S, Siu V, Ades A, Dubbs H, Raible S, Kaur M, Salzano E, Jackson L, Deardorff M, Kline A, Summar M, Muenke M, Linguraru MG, and Krantz ID
- Subjects
- Abnormalities, Multiple epidemiology, Abnormalities, Multiple physiopathology, Adolescent, Adult, Child, Child, Preschool, Chondroitin Sulfate Proteoglycans genetics, Chromosomal Proteins, Non-Histone genetics, De Lange Syndrome epidemiology, De Lange Syndrome physiopathology, Face physiopathology, Female, Humans, Image Processing, Computer-Assisted, Infant, Infant, Newborn, Intellectual Disability epidemiology, Intellectual Disability physiopathology, Male, Mutation, Phenotype, Racial Groups genetics, Young Adult, Abnormalities, Multiple genetics, Cell Cycle Proteins genetics, De Lange Syndrome genetics, Intellectual Disability genetics
- Abstract
Cornelia de Lange syndrome (CdLS) is a dominant multisystemic malformation syndrome due to mutations in five genes-NIPBL, SMC1A, HDAC8, SMC3, and RAD21. The characteristic facial dysmorphisms include microcephaly, arched eyebrows, synophrys, short nose with depressed bridge and anteverted nares, long philtrum, thin lips, micrognathia, and hypertrichosis. Most affected individuals have intellectual disability, growth deficiency, and upper limb anomalies. This study looked at individuals from diverse populations with both clinical and molecularly confirmed diagnoses of CdLS by facial analysis technology. Clinical data and images from 246 individuals with CdLS were obtained from 15 countries. This cohort included 49% female patients and ages ranged from infancy to 37 years. Individuals were grouped into ancestry categories of African descent, Asian, Latin American, Middle Eastern, and Caucasian. Across these populations, 14 features showed a statistically significant difference. The most common facial features found in all ancestry groups included synophrys, short nose with anteverted nares, and a long philtrum with thin vermillion of the upper lip. Using facial analysis technology we compared 246 individuals with CdLS to 246 gender/age matched controls and found that sensitivity was equal or greater than 95% for all groups. Specificity was equal or greater than 91%. In conclusion, we present consistent clinical findings from global populations with CdLS while demonstrating how facial analysis technology can be a tool to support accurate diagnoses in the clinical setting. This work, along with prior studies in this arena, will assist in earlier detection, recognition, and treatment of CdLS worldwide., (© 2019 Wiley Periodicals, Inc.)
- Published
- 2019
- Full Text
- View/download PDF
14. Profile of thoracic trauma victims submitted to chest drainage.
- Author
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Broska CA Júnior, Botelho AB, Linhares AC, DE-Oliveria MS, Veronese G, Naufel CR Júnior, Batista LC, and Diogo MAK
- Subjects
- Adult, Cross-Sectional Studies, Female, Humans, Male, Retrospective Studies, Drainage, Thoracic Injuries therapy
- Abstract
Objective: to describe and compare the variables involved in trauma victims undergoing thoracic drainage., Methods: we conducted a retrospective, analytical, descriptive, cross-sectional study, with medical records of patients attended at the Trauma Service of the Curitiba Evangelical University Hospital between February 2011 and January 2014., Results: there were 488 patients undergoing chest drainage, 84.7% men and 15.3% women, with an average age of 38.2 years. Attendances usually occurred at night, without predominance between open or closed mechanism, gender or age group. The majority of patients with thoracic trauma requiring drainage were diagnosed by anamnesis and physical examination (41.1%) and drained in the emergency room (80.8%). Most of the patients (66.2%) had another associated lesion, mostly some abdominal viscera. Complications were present in 16.6% (81 patients), most of them due to drainage positioning error (9.2%). The mean hospital stay was 15 days and drainage lasted for an average of 8.1 days, with no statistical difference between open and closed trauma. The clinical outcome was discharge in most cases., Conclusion: the profile of patients with thoracic trauma is that of young men, attended at night, with some other associated lesion. Although diagnosis and treatment were rapid and most often without the need for complex examinations, the time of drainage, hospitalization and complications were higher than in the literature, which can be explained by the drainage being made at the Emergency Room and the presence of associated injuries.
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- 2017
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15. Evaluation of pharmacokinetics and efficacy of ivermectin following oral administration in dogs against experimental infection of Ctenocephalides felis felis and Rhipicephalus sanguineus.
- Author
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Magalhães VS, Cid YP, Ferreira TP, Medeiros DMV, de S O Batista LC, Correia TR, Albert ALM, and Scott FB
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- Administration, Oral, Animals, Antiparasitic Agents administration & dosage, Dog Diseases parasitology, Dogs, Flea Infestations prevention & control, Flea Infestations veterinary, Ivermectin administration & dosage, Ivermectin blood, Male, Tick Infestations prevention & control, Tick Infestations veterinary, Antiparasitic Agents pharmacokinetics, Ctenocephalides drug effects, Dog Diseases prevention & control, Ivermectin pharmacokinetics, Rhipicephalus sanguineus drug effects
- Abstract
With the increasing number of pets in home the human-animal relationship is increasingly close and care about control disease growing. Ivermectin (IVM) is frequently used because its proven safety. IVM is recommended for the treatment of demodectic scabies and prevention of heartworm in dogs, but informally is extremely used to control of Ctenocephalides felis felis and Rhipicephalus sanguineus. The aim of this study is evaluate the use of IVM in dogs, by the oral route at 0.6μg/kg dose, against experimental infection of these parasites using the construction of the plasma concentration curve and efficacy study. A IVM quantification method in canine plasma using HPLC-FL was developed and validated based on RE n°899/03 ANVISA. The samples collected during the efficacy test was analyzed by this validated method and prove C
max of 350ng/mL at 4h (tmax ) and AUC of 8411ng/h/mL. Spite of formulation have shown good absorption, the highest efficiency values found for Rhipcephalus sanguineus and Ctenocephalides felis felis were very low, 35% and 67% respectively, demonstrating this not be the most appropriate treatment for the control of these parasites., (Copyright © 2016 Elsevier B.V. All rights reserved.)- Published
- 2016
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16. In vitro efficacy of essential oils and extracts of Schinus molle L. against Ctenocephalides felis felis.
- Author
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Batista LC, Cid YP, De Almeida AP, Prudêncio ER, Riger CJ, De Souza MA, Coumendouros K, and Chaves DS
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- Animals, Cats, Fruit chemistry, Lethal Dose 50, Oils, Volatile chemistry, Ovum drug effects, Plant Extracts chemistry, Plant Leaves chemistry, Saccharomyces cerevisiae drug effects, Anacardiaceae chemistry, Ctenocephalides drug effects, Insecticides pharmacology, Oils, Volatile pharmacology, Plant Extracts pharmacology
- Abstract
Extracts and essential oils from plants are important natural sources of pesticides. These compounds are considered an alternative to control ectoparasites of veterinary importance. Schinus molle, an endemic species of Brazil, produces a high level of essential oil and several other compounds. The aim of this work was to determinate the chemical composition of extracts and essential oils of S. molle and further to evaluate the activity against eggs and adults of Ctenocephalides felis felis, a predominant flea that infests dogs and cats in Brazil. In an in vitro assay, the non-polar (n-hexane) extract showed 100% efficacy (800 µg cm(-2); LD50 = 524·80 µg cm(-2)) at 24 and 48 h. Its major compound was lupenone (50·25%). Essential oils from fruits and leaves were evaluated, and had 100% efficacy against adult fleas at 800 µg cm(-2) (LD50 = 353·95 µg cm(-2)) and at 50 µg cm(-2) (LD50 = 12·02 µg cm(-2)), respectively. On the other hand, the essential oil from fruits and leaves was not active against flea eggs. This is the first study that reports the insecticidal effects of essential oils and extracts obtained from Schinus molle against Ctenocephalides felis felis.
- Published
- 2016
- Full Text
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17. Tumor de corpo carotídeo (paraganglioma): relato de dois casos submetidos a tratamento cirúrgico.
- Author
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Mesquita N Junior, Silva RS, Ribeiro JHA, Batista LC, Bringhentti EMS, de Souza BBB, and Cabral LCDM
- Abstract
Competing Interests: Conflito de interesse: Os autores declararam não haver conflitos de interesse que precisam ser informados.
- Published
- 2016
- Full Text
- View/download PDF
18. Immediate graft histological assay, post pig's liver transplantation.
- Author
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Brito MV, Araújo MT, Cunha LC, Oliveira AJ, Rodrigues JB, and Brito NB
- Subjects
- Animals, Biopsy, Fatty Liver, Female, Models, Animal, Necrosis, Portal System pathology, Postoperative Complications, Reproducibility of Results, Swine, Time Factors, Liver pathology, Liver Transplantation methods
- Abstract
Purpose: To describe the vascular and tissue histopathological changes in seven sequential experimental liver transplantations in pigs., Methods: Fourteen female pigs, Sus domesticus species, with body mass between 5 and 8 kg were utilized. After the end of all anastomoses of the graft implantation in the receptor, the animal was monitored for 30 minutes, and at its end one of the biopsies was collected for histological analysis. The histological criteria utilized were: lytic hepatocyte necrosis, density of septal and portal inflammatory infiltrated, sinusoidal congestion and hemorrhage. The analysis was performed separately for the portal region in zone 1, 2 and 3., Results: Among the structural changes undergone by the graft, those with greater frequency and intensity were vascular congestion and steatosis, which stood out in transplantations 5, 6 and 7., Conclusions: The technique demonstrated vascular alterations represented by vasocongestion, edema and minimum inflammatory reaction. In relation to the parenchyma, was observed macrovacuolar pan-acinar steatosis, focal lytic and occasional hemorrhages, beyond the accumulation of hemosiderin in Kuppfer's cells.
- Published
- 2013
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19. Tension band wire fixation for valgus osteotomies of the proximal femur: a biomechanical study of three configurations of fixation.
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Volpon JB, Batista LC, Shimano MM, and Moro CA
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- Animals, Compressive Strength, Elasticity, Equipment Design, Female, Femur surgery, Hip Joint physiopathology, Hip Joint surgery, Internal Fixators, Joint Instability physiopathology, Male, Osteotomy adverse effects, Range of Motion, Articular, Stress, Mechanical, Sus scrofa, Torque, Torsion Abnormality surgery, Bone Wires, Femur physiopathology, Joint Instability prevention & control, Osteotomy instrumentation, Osteotomy methods
- Abstract
Background: A variety of techniques can be used to achieve stabilization of femoral valgus osteotomies in children, but what is lacking is a versatile fixation system that associates stability and versatility at different ages and for different degrees of deformity., Methods: Mechanical tests of three configurations used to fix femoral valgus osteotomies, based on the tension band wire principle, were carried out. A 30 degrees wedge valgus osteotomy was performed at the subtrochanteric level in 60 swine femurs and fixed with three different systems. In Group 1, two Kirschner wires (K wire) were introduced from the tip of the greater trochanter to the medial cortex, crossing the osteotomy. A flexible steel wire was anchored to the K wires into holes in the lateral cortex and tightened to form a tension band. The same setup was used in Group 2, but two additional smooth K wires were inserted into the lateral surface of the greater trochanter and driven to the femoral head with the distal extremities bent and tied around the bone shaft. In Group 3, the fixation was similar to that in Group 2, but the ascending K wires were introduced below the osteotomy level, crossing the osteotomy. Mechanical tests in bending-compression and torsion were used to access the stability., Findings: The torsional relative stiffness was 116% greater for Group 3 (0.27 Nm/degree) and no significant difference was found between Group 1 (0.10Nm/degree) and Group 2 (0.12 Nm/degree). The average torque was 103% higher for Group 3 (1.86 Nm). Stiffness in bending-compression was significantly higher in Group 3 (508 x 10(3) N/m) than in Group 1 (211 x 10(3) N/m) and Group 2 (219 x 10(3) N/m)., Interpretation: Fixation as used in Group 3 was significantly more stable, both in torsion and bending-compression tests, than the other two techniques.
- Published
- 2008
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20. The risk is in the air: Intranasal administration of MPTP to rats reproducing clinical features of Parkinson's disease.
- Author
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Prediger RD, Batista LC, Medeiros R, Pandolfo P, Florio JC, and Takahashi RN
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- Administration, Intranasal, Animals, Behavior, Animal drug effects, Blotting, Western methods, Brain drug effects, Brain physiopathology, Disease Models, Animal, Dopamine metabolism, Male, Maze Learning physiology, Motor Activity drug effects, Olfactory Bulb drug effects, Olfactory Bulb physiopathology, Parkinsonian Disorders chemically induced, Rats, Rats, Wistar, Time Factors, Tyrosine 3-Monooxygenase metabolism, 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine administration & dosage, Neurotoxins administration & dosage, Parkinsonian Disorders physiopathology, Risk
- Abstract
Many studies have shown that deficits in olfactory and cognitive functions precede the classical motor symptoms seen in Parkinson's disease (PD) and that olfactory testing may contribute to the early diagnosis of this disorder. Although the primary cause of PD is still unknown, epidemiological studies have revealed that its incidence is increased in consequence of exposure to certain environmental toxins. In the present study, we demonstrated that rats treated with intranasal infusion of the neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) at low concentrations (0.1 mg/nostril) suffered progressive impairments in olfactory, cognitive and motor functions that were assessed with the olfactory discrimination, Morris water maze and open field tests, respectively. Moreover, intranasal administration of MPTP reduced the expression of the enzyme tyrosine hydroxylase in the olfactory bulb and substantia nigra of rats, resulting in a significant reduction of dopamine concentration in the olfactory bulb, prefrontal cortex and striatum, but not in the hippocampus. These results reinforce the notion that the olfactory system represents a particularly sensitive route for the transport of neurotoxins into the central nervous system that may be related to the etiology of PD. In addition, the time course of the olfactory, cognitive and motor impairments verified in rats treated intranasally with MPTP, which appears to be correlated with different stages of the human PD, suggest that the MPTP intranasal model in rats may provide new insights into the underlying mechanisms of PD pathogenesis.
- Published
- 2006
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21. Activation of adenosine A1 receptors reduces anxiety-like behavior during acute ethanol withdrawal (hangover) in mice.
- Author
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Prediger RD, da Silva GE, Batista LC, Bittencourt AL, and Takahashi RN
- Subjects
- Adenosine analogs & derivatives, Adenosine pharmacology, Adenosine A1 Receptor Agonists, Alcoholic Intoxication blood, Analysis of Variance, Animals, Anxiety drug therapy, Central Nervous System Depressants blood, Disease Models, Animal, Dose-Response Relationship, Drug, Drug Interactions, Ethanol blood, Exploratory Behavior drug effects, Male, Maze Learning drug effects, Mice, Time Factors, Alcoholic Intoxication complications, Alcoholic Intoxication etiology, Anxiety etiology, Central Nervous System Depressants toxicity, Ethanol toxicity, Receptor, Adenosine A1 physiology
- Abstract
Elevated signs of anxiety are observed in both humans and rodents during withdrawal from chronic as well as acute ethanol exposure, and it represents an important motivational factor for ethanol relapse. Several reports have suggested the involvement of brain adenosine receptors in different actions produced by ethanol such as motor incoordination and hypnotic effects. In addition, we have recently demonstrated that adenosine A1 receptors modulate the anxiolytic-like effect induced by ethanol in mice. In the present study, we evaluated the potential of adenosine A1 and A2A receptor agonists in reducing the anxiety-like behavior during acute ethanol withdrawal (hangover) in mice. Animals received a single intraperitoneal administration of saline or ethanol (4 g/kg) and were tested in the elevated plus maze after an interval of 0.5-24 h. The results indicated that hangover-induced anxiety was most pronounced between 12 and 18 h after ethanol administration, as indicated by a significant reduction in the exploration of the open arms of the maze. At this time interval, ethanol was completely cleared. The acute administration of 'nonanxiolytic' doses of adenosine and the selective adenosine A1 receptor agonist 2-chloro-N6-cyclopentyladenosine (CCPA), but not the adenosine A2A receptor agonist N6-[2-(3,5-dimethoxyphenyl)-2-(2-methylphenyl)ethyl]adenosine (DPMA), at the onset of peak withdrawal (18 h), reduced this anxiogenic-like response. In addition, the effect of CCPA on the anxiety-like behavior of ethanol hangover was reversed by pretreatment with the selective adenosine A1 receptor antagonist 8-cyclopentyl-1,3-dipropylxanthine (DPCPX). These results reinforce the notion of the involvement of adenosine receptors in the anxiety-like responses and indicate the potential of adenosine A1 receptor agonists to reduce the anxiogenic effects during ethanol withdrawal.
- Published
- 2006
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22. Blockade of adenosine and dopamine receptors inhibits the development of rapid tolerance to ethanol in mice.
- Author
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Batista LC, Prediger RD, Morato GS, and Takahashi RN
- Subjects
- Animals, Brain drug effects, Male, Mice, Drug Tolerance, Ethanol pharmacology, Purinergic P1 Receptor Antagonists, Receptors, Dopamine drug effects
- Abstract
Rationale: Several reports have suggested the involvement of brain adenosine and dopamine receptors in different actions produced by ethanol such as motor incoordination or anxiolytic, hypnotic and reinforcing effects. The co-localization and interaction between adenosine and dopamine receptors in different brain regions has also been well documented. However, few studies have demonstrated the involvement of these mechanisms in the tolerance induced by ethanol., Objectives: The aim of the present study was to evaluate the role of adenosine and dopamine receptors in the development of rapid tolerance to ethanol-induced motor incoordination in mice., Methods: In connection with the rota-rod apparatus, the effects of acute administration of the adenosine receptor antagonists caffeine (non-selective), 8-cyclopentyl-1,3-dipropylxanthine (DPCPX, adenosine A1 receptor antagonist) and 4-(2-[7-amino-2-{2-furyl}{1,2,4}triazolo-{2,3-a}{1,3,5}triazin-5-yl-amino]ethyl)phenol (ZM241385, adenosine A2A receptor antagonist), together with R(+)-7-chloro-8-hydroxy-3-methyl-1-phenyl-2,3,4,5-tetrahydro-1H-3-benzazepine (SCH23390, dopamine D1 receptor antagonist) and sulpiride (dopamine D2 receptor antagonist), alone or in combination with ethanol (2.25 g/kg, i.p.), were studied. Twenty-four hours after, all animals were re-tested on the rota-rod after receiving the same dose of ethanol., Results: The repeated administration of ethanol promoted a significant reduction of motor impairment on day 2 (i.e. rapid tolerance). This effect was blocked by caffeine (3.0-30.0 mg/kg, i.p.), DPCPX (3.0-6.0 mg/kg, i.p.) or SCH23390 (0.01-0.03 mg/kg, s.c.), but not with ZM241385 (0.5-1.0 mg/kg, i.p.) or sulpiride (1.0-3.0 mg/kg, i.p.)., Conclusions: Our results suggest that the rapid tolerance to ethanol-induced motor impairment in mice may be modulated by adenosine A1 receptors and dopamine D1 receptors.
- Published
- 2005
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23. Caffeine reverses age-related deficits in olfactory discrimination and social recognition memory in rats. Involvement of adenosine A1 and A2A receptors.
- Author
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Prediger RD, Batista LC, and Takahashi RN
- Subjects
- Aging drug effects, Animals, Behavior, Animal drug effects, Behavior, Animal physiology, Central Nervous System Stimulants administration & dosage, Discrimination Learning drug effects, Discrimination Learning physiology, Dose-Response Relationship, Drug, Male, Rats, Rats, Wistar, Recognition, Psychology drug effects, Smell drug effects, Social Behavior, Aging physiology, Caffeine administration & dosage, Memory drug effects, Receptor, Adenosine A1 metabolism, Receptors, Adenosine A2 metabolism, Recognition, Psychology physiology, Smell physiology
- Abstract
Caffeine, a non-selective adenosine receptor antagonist, has been suggested as a potential drug to counteract age-related cognitive decline since critical changes in adenosinergic neurotransmission occur with aging. In the present study, olfactory discrimination and short-term social memory of 3, 6, 12 and 18 month-old rats were assessed with the olfactory discrimination and social recognition tasks, respectively. The actions of caffeine (3.0, 10.0 and 30.0 mg/kg, i.p.), the A1 receptor antagonist DPCPX (1.0 and 3.0 mg/kg, i.p.) and the A2A receptor antagonist ZM241385 (0.5 and 1.0 mg/kg, i.p.) in relation to age-related effects on olfactory functions were also studied. The 12 and 18 month-old rats exhibited significantly impaired performance in both models, demonstrating deficits in their odor discrimination and in their ability to recognize a juvenile rat after a short period of time. Acute treatment with caffeine or ZM241385, but not with DPCPX, reversed these age-related olfactory deficits. The present results suggest the participation of adenosine receptors in the control of olfactory functions and confirm the potential of caffeine for the treatment of aged-related cognitive decline.
- Published
- 2005
- Full Text
- View/download PDF
24. Adenosine A1 receptors modulate the anxiolytic-like effect of ethanol in the elevated plus-maze in mice.
- Author
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Prediger RD, Batista LC, and Takahashi RN
- Subjects
- Adenosine pharmacology, Adenosine A1 Receptor Agonists, Adenosine A1 Receptor Antagonists, Adenosine A2 Receptor Antagonists, Analysis of Variance, Animals, Anxiety physiopathology, Anxiety prevention & control, Anxiety psychology, Caffeine pharmacology, Dose-Response Relationship, Drug, Drug Synergism, Male, Mice, Triazines pharmacology, Triazoles pharmacology, Xanthines pharmacology, Adenosine analogs & derivatives, Anti-Anxiety Agents pharmacology, Ethanol pharmacology, Maze Learning drug effects, Receptor, Adenosine A1 physiology
- Abstract
The anxiolytic property of ethanol is generally accepted to be an important motivational factor for its consumption and the development of alcohol dependence. Recent studies suggest that adenosine receptors mediate important actions of ethanol, such as motor incoordination and hypnotic effects. In addition, several lines of evidence support the involvement of adenosine in anxiety. The aim of the present study was to evaluate the role of adenosine receptors in the anxiolytic-like effect of ethanol in mice. The effects of acute administration of the adenosine receptor antagonists caffeine (nonselective), 8-cyclopentyl-1,3-dipropylxanthine (DPCPX, adenosine A1 receptor antagonist) and 4-(2-[7-amino-2-[2-furyl][1,2,4]triazolo-[2,3-a][1,3,5]triazin-5-yl-amino]ethyl)phenol (ZM241385, adenosine A(2A) receptor antagonist), together with the adenosine A1 receptor agonist 2-chloro-N6-cyclopentyladenosine (CCPA), and their interaction with ethanol in the elevated plus-maze test in mice were studied. The highest doses of caffeine (30.0 mg/kg, i.p.) and DPCPX (6.0 mg/kg, i.p.) produced an anxiogenic-like effect, while CCPA administration (0.25 mg/kg, i.p.) showed an anxiolytic-like activity. The prior administration of "non-anxiogenic" doses of caffeine (10.0 mg/kg, i.p.) and DPCPX (3.0 mg/kg, i.p.), but not ZM241385 (1.0 mg/kg, i.p.), significantly reduced the anxiolytic-like effect of ethanol (1.2 g/kg, i.p.). Moreover, anxiolytic-like response was observed by the co-administration of "non-anxiolytic" doses of CCPA (0.125 mg/kg) and ethanol (0.6 g/kg). These results reinforce the involvement of adenosine in anxiety and suggest that the activation of adenosine A1 receptors, but not adenosine A(2A) receptors, mediate the anxiolytic-like effect induced by ethanol in mice.
- Published
- 2004
- Full Text
- View/download PDF
25. Facilitation of short-term social memory by ethanol in rats is mediated by dopaminergic receptors.
- Author
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Prediger RD, Batista LC, Miyoshi E, and Takahashi RN
- Subjects
- Adrenergic Uptake Inhibitors pharmacology, Analysis of Variance, Animals, Behavior, Animal, Dopamine Antagonists pharmacology, Dose-Response Relationship, Drug, Drug Interactions, Male, Neuropsychological Tests, Rats, Rats, Wistar, Reserpine pharmacology, Time Factors, Central Nervous System Depressants pharmacology, Ethanol pharmacology, Memory, Short-Term drug effects, Receptors, Dopamine physiology, Social Behavior
- Abstract
Ethanol is a drug that has apparently opposite effects on memory processes depending on when it is given relative to the task, as well as the nature of the task under study. Recently, we demonstrated that acute low doses of ethanol (0.5 and 1.0 g/kg, i.p.) improve the short-term social memory in rats in a specific and time-dependent manner, and that this action is, at least in part, related to opioid, but not to muscarinic receptors. In the present study, we evaluated whether this positive effect of ethanol on the short-term memory of rats is related to a reducing impact of interference during the task through two different procedures: the introduction of an unfamiliar juvenile rat or the placing of the adult rat in the open field during the inter-exposure interval. The actions of reserpine (0.4 and 0.8 mg/kg, s.c.), haloperidol (0.05 and 0.2 mg/kg, i.p.), the D2 receptor antagonist sulpiride (20.0 and 50.0 mg/kg, i.p.) and the D1 receptor antagonist SCH 23390 (0.01 and 0.03 mg/kg, s.c.) and their interaction with ethanol (1.0 g/kg, i.p.) in relation to short-term memory were also studied. The administration of ethanol (1.0 g/kg, i.p.), immediately after the end of the first presentation, did not reduce the effect on social memory of the introduction of an unfamiliar juvenile or placing the adult rat in the open field during the inter-exposure interval. The facilitatory effect of ethanol on social memory was inhibited by the pretreatment with reserpine and it was antagonized by the administration of haloperidol or sulpiride, but not by SCH 23390. These results indicate that the facilitation of short-term social memory by ethanol is not related to a reduction in the deleterious impact of interference and that this action of ethanol is mediated, at least in part, by D2 receptors, but not by D1 dopaminergic receptors.
- Published
- 2004
- Full Text
- View/download PDF
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