1. Characterization of systemic allergenicity of tropomyosin from shrimp ( <scp> Macrobrachium nipponense </scp> ) and anaphylactic reactions in digestive tract
- Author
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Hongbing Chen, Xuanyi Meng, Xin Hu, Xiwei Hua, Huming Shao, and Yanhai Xie
- Subjects
Basophil cell ,030309 nutrition & dietetics ,Ileum ,Shellfish Hypersensitivity ,Tropomyosin ,Pharmacology ,Basophil ,medicine.disease_cause ,digestive system ,Jejunum ,Mice ,03 medical and health sciences ,chemistry.chemical_compound ,Th2 Cells ,0404 agricultural biotechnology ,Immune system ,medicine ,Animals ,Humans ,Mice, Inbred BALB C ,0303 health sciences ,Nutrition and Dietetics ,digestive, oral, and skin physiology ,04 agricultural and veterinary sciences ,Allergens ,Immunoglobulin E ,040401 food science ,Gastrointestinal Tract ,medicine.anatomical_structure ,chemistry ,Immunoglobulin G ,Allergic response ,Duodenum ,Cytokines ,Female ,Palaemonidae ,Agronomy and Crop Science ,Histamine ,Food Science ,Biotechnology - Abstract
Tropomyosin (TM) is the major allergen of crustaceans. The allergenicity of TM from Macrobrachium nipponense (MnTM) and the anaphylactic reaction in the digestive tract are still unclear. The aim of this study was to evaluate the allergenicity of MnTM and the anaphylactic reaction in the digestive tract.Serum IgE and IgG1 binding ability in the TM group were significantly higher than those in the PBS and CT groups (P 0.01) and CP group (P 0.05), while serum IgG and IgG2a binding ability showed no obvious difference between the four groups (P 0.05). The levels of cytokines IL-4, IL-5 and IL-13 in TM and CP groups were significantly higher than those in PBS and CT groups. Histamine and β-hexosaminidase in the TM and CP groups from basophil cell models were significantly higher than those in the PBS group. The highest mRNA expression of IL-4 and IL-13 was in the jejunum from TM-sensitized mice. Histopathological analysis showed that more immune cells infiltrated into the jejunum than the duodenum and ileum from the TM-sensitized mice.MnTM could promote an allergic response in mice and lead to degranulation in basophil cells. The jejunum was more easily affected by MnTM than duodenum and ileum, and the jejunum may be the major site of allergic response in the digestive tract. © 2020 Society of Chemical Industry.
- Published
- 2020
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